Pharmaceutical compositions comprising RPL554 in HFA-134a for administration by inhalation

Abstract

To provide a liquid pharmaceutical composition suitable for administration by inhalation.SOLUTION: Provided is a liquid pharmaceutical composition for use in the treatment of the human or animal body, which is suitable for administration by inhalation, comprising: (i)a suspension of particles comprising 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinoline-4-one (RPL554); and (ii) a diluent which is 1,1,1,2-tetrafluoroethane (HFA-134a), the liquid pharmaceutical composition containing less than 0.05 wt.% of a surfactant to the total weight of the composition.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a liquid pharmaceutical composition comprising a respiratory medication, and to a pressurized metered dose inhaler (pMDI) comprising said liquid pharmaceutical composition. [Background technology]

[0002] RPL554 (9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one) is a dual PDE3 / PDE4 inhibitor and is described in WO00 / 58308. As a combined PDE3 / PDE4 inhibitor, RPL554 has both anti-inflammatory and bronchodilatory effects and is useful in the treatment of respiratory diseases such as asthma and chronic obstructive pulmonary disease (COPD). The structure of RPL554 is shown below.

[0003] [ka]

[0004] RPL554 is typically administered by inhalation, given its effectiveness in treating respiratory diseases. Administration of RPL554 by nebulizer is known (WO2016 / 042313). However, it is often desirable to administer respiratory drugs using a pressurized metered dose inhaler (pMDI).

[0005] pMDI formulations comprising salts of RPL554 are contemplated in WO2015 / 173551. pMDI formulations comprising combinations of RPL554 with a second active agent and a surfactant are described in WO2014 / 140648.

[0006] Known pMDI formulation strategies can involve the use of a wide range of different propellants, excipients, co-solvents and surfactants, and may take the form of suspensions, solutions or mixtures thereof. For example, pMDI formulations may include one or more propellants such as alkanes (e.g., propane, n-butane, isobutane, n-pentane, isopentane, neopentane), hydrofluoroalkanes (HFAs, e.g., HFA-227, HFA-134a, and HFA 152a), ethers (e.g., dimethyl ether), and hydrofluoroolefins (HFOs, e.g., HFO-1234ze and HFO-1234yf). Co-solvents such as ethanol and water are typically included in pMDI formulations. Excipients typically included in pMDI formulations include antioxidants, preservatives, humectants, chelating agents, emulsifiers, flavorings, buffers, lubricants, suspending agents, and isotonicity agents.

[0007] Drugs with low solubility in diluents are often formulated as suspensions. In such suspension formulations, an important consideration is the uniform dispersion of drug particles. Accumulation, phase separation, and aggregation can lead to metered dose variations. Therefore, additional excipients such as surfactants and cosolvents are usually used in suspension formulations to improve the suspension properties. An additional consideration for suspension formulations is that of particle size distribution. The particle size distribution of the drug is usually maintained in the respirable range (typically less than 5 μm).

[0008] Each of the components included in a pMDI can have a number of effects on the stability and efficacy of the formulation that depend largely on the identity of the drug being formulated. Such effects cannot be predicted with any certainty in advance. Correct formulation of a drug for use in a pMDI is important to ensure that the formulation can be successfully used in clinical practice. Summary of the Invention

[0009] It is a surprising discovery of the present invention that a liquid pharmaceutical composition comprising particles of RPL554 and HFA-134a (1,1,1,2-tetrafluoroethane) as a diluent / propellant, substantially free of surfactants, is particularly advantageous for the delivery of RPL554 by pressurized metered dose inhalers. Numerous advantages have been found associated with this liquid pharmaceutical composition. It has been found that a particular combination of RPL554 and HFA-134a leads to a suspension pMDI formulation with favorable suspension properties and reduced aggregation. It has also been found that a chemically and physically stable formulation may be achieved without the need for the presence of a surfactant.

[0010] The ability to omit surfactants from the compositions of the present invention is highly advantageous, since RPL554 is likely to be used in patients suffering from conditions such as COPD and asthma, who are at high risk of allergic inflammatory reactions to excipients such as surfactants.Furthermore, in view of the teachings of WO2014 / 140648, which suggest that surfactants are necessary to achieve a suitable pMDI, it is surprising that suitable long-term stability can be achieved without surfactants and without particle accumulation or aggregation of the active ingredient.

[0011] It has also been unexpectedly found that particles of RPL554 are vulnerable to Ostwald ripening (particle growth in suspension) in the presence of co-solvents such as ethanol.It is important to avoid Ostwald ripening, since the increase in particle size can reduce the respirable fraction of particles present in the formulation.The liquid pharmaceutical formulation of the present invention has been found to avoid Ostwald ripening of RPL554 particles.

[0012] The present invention provides a liquid pharmaceutical composition suitable for administration by inhalation comprising: (i) a suspension of particles comprising 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one (RPL554); and (ii) a diluent which is 1,1,1,2-tetrafluoroethane (HFA-134a), wherein the liquid pharmaceutical composition is substantially free of surfactant.

[0013] The present invention further provides a pressurized metered dose inhaler comprising a liquid pharmaceutical composition of the present invention.

[0014] The present invention also provides a liquid pharmaceutical composition of the present invention for use in the treatment of the human or animal body.The liquid pharmaceutical composition of the present invention may be used in the treatment or prevention of a disease or condition selected from asthma, allergic asthma, hay fever, allergic rhinitis, bronchitis, emphysema, bronchiectasis, chronic obstructive pulmonary disease (COPD), adult respiratory distress syndrome (ARDS), steroid-resistant asthma, severe asthma, childhood asthma, cystic fibrosis, lung fibrosis, pulmonary fibrosis, interstitial lung disease, skin disorders, atopic dermatitis, psoriasis, eye inflammation, cerebral ischemia, inflammatory diseases and autoimmune diseases.

[0015] The present invention also provides a method for treating or preventing a disease or condition as defined herein in a subject, comprising administering to said subject an effective amount of a liquid pharmaceutical composition of the present invention. [Brief description of the drawings]

[0016] [Figure 1] Figure 1 shows a schematic of the visual assessment of suspension performance in pressure-rated glass vials. [Diagram 2] FIG. 2 shows the sedimentation scores for each product strength propellant mix. [Diagram 3]FIG. 3 shows a diagram of the test canister 24 hours after placing the dip tube in the liquid phase. [Figure 4] FIG. 4 shows a graph of RPL554 solubility with increasing concentrations of ethanol, with and without oleic acid. [Diagram 5] FIG. 5 shows the mean fine particle dose versus product strength for pMDI formulations. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0017] Detailed Description of the Invention The liquid pharmaceutical composition of the present invention comprises a suspension of particles of 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one (RPL554). For the avoidance of doubt, RPL554 is in the form of the free base. Although the majority of the particles present in a liquid pharmaceutical composition typically remain suspended in the diluent, some or all of the particles in a liquid pharmaceutical composition of the present invention may have settled to the bottom of a container containing the liquid pharmaceutical composition, for example after a period of storage. The particles may be resuspended in any suitable manner, for example by agitation of the liquid pharmaceutical composition (e.g. by shaking a storage container containing the liquid pharmaceutical composition).

[0018] The diluent in the liquid pharmaceutical composition is 1,1,1,2-tetrafluoroethane, known as HFA-134a and having the formula CH2FCF3. The diluent also acts as a propellant. Typically, HFA-134a is the only diluent in the liquid pharmaceutical composition. In another embodiment, the liquid pharmaceutical composition includes a diluent that includes more than 90% by weight HFA-134a, based on the total weight of the diluent in the liquid pharmaceutical composition. The diluent may include more than 95% by weight HFA-134a, more than 98% by weight, or more than 99.5% by weight HFA-134a, based on the total weight of the diluent in the liquid pharmaceutical composition. The diluent may consist essentially of HFA-134a. A composition that consists essentially of a component typically includes only that component and any other components that do not significantly affect the substantial properties of the component of which the composition is substantially composed. Typically, the diluent consists of HFA-134a. The diluent corresponds to the liquid component of the liquid pharmaceutical composition.

[0019] A liquid pharmaceutical composition is substantially free of surfactants. An article is "substantially free" of a particular ingredient if it contains less than 0.5% by weight, preferably less than 0.1% by weight, more preferably less than 0.01% by weight, e.g., less than 0.001% by weight, of the particular ingredient relative to the total weight of the composition. Typically, liquid pharmaceutical compositions do not contain surfactants.

[0020] Examples of surfactants include nonionic surfactants, anionic surfactants, cationic surfactants or zwitterionic surfactants. For the avoidance of doubt, the liquid pharmaceutical composition may therefore be formulated with lecithin, oleic acid, polyvinylpyrrolidone K25, polyvinyl alcohol, oligolactic acid, sodium dioctyl sulfosuccinate, polyoxyethylene glycol alkyl ethers (e.g., PEG 300, PEG 600, PEG 1000, Brij 30, Brij 35, Brij 56, Brij 76 and Brij 97), polypropylene glycols (e.g., PPG 2000), glucoside alkyl ethers, polyoxyethylene glycol octylphenol ethers, polyoxyethylene glycol alkylphenol ethers, glycerol alkyl esters, polyoxyethylene glycol sorbitan alkyl esters (polysorbates, e.g., Polysorbate 20, Polysorbate 60, and Polysorbate 80), sorbitan alkyl esters (e.g., Sorbitan Monolaurate (Span 20), Sorbitan Monooleate (Span 80), and Sorbitan Trioleate (Span 85)), cocamide MEA, cocamide DEA, dodecyl dimethylamine oxide, block copolymers of polyethylene glycol and polypropylene glycol (poloxamers), block copolymers of polyethylene glycol and polypropylene oxide (e.g., Pluronic surfactants), and polyethoxylated tallow amine (POEA).

[0021] In a preferred embodiment, the liquid pharmaceutical composition of the present invention is substantially free of ethanol.

[0022] More preferably, the liquid pharmaceutical composition is substantially free of any additional excipients selected from co-solvents and surfactants. The liquid pharmaceutical composition more typically does not include any co-solvents and does not include any surfactants.

[0023] Examples of co-solvents include ethanol, pentane, water, isopropanol, glycerol and propylene glycol.

[0024] In a preferred embodiment, the liquid pharmaceutical composition is substantially free of any additional excipients, which may be, for example, surfactants or cosolvents as described above, or excipients selected from antioxidants, preservatives, wetting agents, solubilizers, emulsifiers, flavoring agents, chelating agents, humectants, isotonicity agents, pH adjusting agents, dispersing agents and suspension aids.

[0025] The liquid pharmaceutical composition may contain less than 0.2% by weight of said surfactant or said additional excipients relative to the total weight of the composition. For example, the liquid pharmaceutical composition may contain less than 0.1% by weight of said surfactant or said additional excipients relative to the total weight of the composition. For example, the liquid pharmaceutical composition may contain less than 0.05% by weight, less than 0.01% by weight, or less than 0.001% by weight of said surfactant or said additional excipients relative to the total weight of the composition.

[0026] Typically, at least 50% by weight, more preferably at least 90% by weight, more preferably at least 99% by weight, of the active pharmaceutical ingredient (API) in a liquid composition of the invention is RPL554, based on the total weight of all APIs.

[0027] More typically, RPL554 is the only active agent in the liquid pharmaceutical composition. In embodiments, the liquid pharmaceutical compositions of the present invention are substantially free of other active agents (eg, muscarinic receptor antagonists or beta adrenergic receptor agonists).

[0028] As explained above, it has been found that the liquid pharmaceutical composition comprising particles of HFA-134a and RPL554 and substantially free of any additional excipients has many advantages.Thus, typically, the liquid pharmaceutical composition is substantially composed of (i) RPL554 and (ii) 1,1,1,2-tetrafluoroethane.More typically, the liquid pharmaceutical composition comprises at least 99.5% by weight of (i) RPL554 and (ii) 1,1,1,2-tetrafluoroethane based on the total weight of the composition.The liquid pharmaceutical composition may, for example, be composed of (i) RPL554 and (ii) 1,1,1,2-tetrafluoroethane.

[0029] A liquid pharmaceutical composition of the invention may, for example, comprise (i) RPL554 in an amount of 0.005 to 5% by weight, and (ii) 1,1,1,2-tetrafluoroethane (HFA-134a) in an amount of 95 to 99.995% by weight, where the weight percentages are based on the total weight of the liquid pharmaceutical composition. Typically, the total amount of RPL554 and HFA-134a is at least 99% by weight, based on the total weight of the composition.

[0030] Particles of RPL554 may be of any size suitable for use in liquid pharmaceutical compositions suitable for inhalation. Typically, particles of RPL554 are micronized particles. For example, particles of RPL554 may have a Dv50 (median particle size by volume) value of 10 μm or less, or about 0.1 μm to about 8 μm.

[0031] Typically, the particles have a particle size distribution with a Dv50 value of about 0.2 μm to about 5 μm. More typically, the particles of RPL554 have a particle size distribution with a Dv50 value of about 0.7 μm to about 3.0 μm. For example, the particles of RPL554 may have a particle size distribution with a Dv50 value of 0.9 μm to 2.7 μm. Often, the particles of RPL554 have a particle size distribution with a Dv50 value of about 1.1 μm to about 2.1 μm.

[0032] The Dv50 value is the median particle size in a volume distribution, i.e. half of the volume of the particles is contained in particles with a diameter smaller than the Dv50 value and half of the volume of the particles is contained in particles with a diameter larger than the Dv50 value. This is a well-known method for describing particle size distribution.

[0033] The particles typically have a particle size distribution with a Dv10 value of about 0.4 μm to about 1.0 μm. The particles typically have a particle size distribution with a Dv90 value of about 2.0 μm to about 4.0 μm. The Dv10 value reflects the particle size, with 10% of the volume of the sample being comprised of particles with a particle size smaller than the Dv10 value. The Dv90 value reflects the particle size, with 90% of the volume of the sample being comprised of particles with a particle size smaller than the Dv90 value.

[0034] The technique used to measure Dv50 value is typically laser diffraction.For example, RPL554 particles typically have a particle size distribution with Dv50 value of about 0.2 μm to about 5 μm as measured by laser diffraction.Particle size distribution analysis can be carried out by laser diffraction using Malvern Spraytec in combination with wet dispersion cell.Typically, the instrument parameters of Malvern Spraytec are as follows:

[0035] · Particles – standard opaque particles; · Refractive index particles - 1.50; · Refractive index (imaginary) - 0.50; · Particle density - 1.00; · Refractive index of dispersant - 1.33; · Controller unit-1000RPM; Measurement type - timed; Initial sampling time - 30 seconds; · Ambiguity - 20%-30%; Dispersant – 1% Polysorbate 20 in deionized water

[0036] Particles of RPL554 may be produced by any pharma- ceutically acceptable size reduction process or particle size controlled manufacturing process. For example, particles may be produced by spray drying a solution of RPL554, by controlled crystallization, or by size reduction of a solid form of RPL554, for example, by air jet milling, mechanical micronization, or media milling.

[0037] The concentration of particles containing RPL554 in the liquid pharmaceutical composition may vary based on the intended dose of active compound per actuation of the inhaler. For example, the concentration of particles may be such that the dose of RPL554 delivered per actuation of the inhaler containing the liquid pharmaceutical composition is between 5 μg / actuation and 1500 μg / actuation. The concentration of particles may be such that the dose of RPL554 delivered per actuation is between 50 μg / actuation and 1000 μg / actuation.

[0038] The concentration of the particles comprising RPL554 in the liquid pharmaceutical composition may be from about 0.01 mg / mL to about 400 mg / mL. The concentration of the particles comprising RPL554 in the liquid pharmaceutical composition is typically from about 0.1 mg / mL to about 200 mg / mL. More typically, the concentration of the particles of RPL554 in the liquid pharmaceutical composition is from about 0.5 mg / mL to about 20 mg / mL.

[0039] For example, the concentration of RPL554 particles in the liquid pharmaceutical composition may be from about 0.1 mg / mL to about 3.0 mg / mL, or from about 0.5 mg / mL to about 2.0 mg / mL. The concentration of RPL554 particles in the liquid pharmaceutical composition may be from about 5.0 mg / mL to about 20 mg / mL, or from about 6.0 mg / mL to about 10 mg / mL.

[0040] The concentration of the particles comprising RPL554 in the liquid pharmaceutical composition may be from about 0.01% w / w to about 5.0% w / w. The concentration of the particles comprising RPL554 in the liquid pharmaceutical composition is typically from about 0.05% w / w to about 2.0% w / w.

[0041] Liquid pharmaceutical compositions are typically suitable for administration by inhalation. Liquid pharmaceutical compositions are more typically suitable for administration by pressurized metered dose inhalers. Pressurized metered dose inhalers are inhalers that use pressurized liquefied propellants to deliver aerosolized doses of liquid pharmaceutical compositions. Pressurized metered dose inhalers typically include a storage container (or vial) that includes the liquid pharmaceutical composition, a metering valve, an actuator, and a mouthpiece.

[0042] The present invention provides a pressurized metered dose inhaler comprising a liquid pharmaceutical composition as defined herein. pMDIs are well known to those skilled in the art and many such devices are commercially available, typical devices being the AeroBid Inhaler System (Forest Pharmaceuticals), Atrovent Inhalation Aerosol (Boehringer Ingelheim), Flovent® (GlaxoSmithKline), Maxair Inhaler (3M), Proventil® Inhaler (Schering) and Serevent® Inhalation Aerosol (GlaxoSmithKline). line).

[0043] A pressurized metered dose inhaler containing a liquid pharmaceutical composition may be configured, upon actuation, to provide an aerosol of the liquid pharmaceutical composition having a median mass aerodynamic diameter (MMAD) of 1.0 μm to 5.0 μm. The MMAD is determined according to official requirements (European Pharmacopoeia (Ph.Eur.) Method Chapter 2.9.18 and United States Pharmacopoeia <601> The impact force may be measured using a Next Generation Impactor in accordance with Chapter 3.

[0044] The present invention provides a liquid pharmaceutical composition as defined herein for use in the treatment of the human or animal body.Typically, the liquid pharmaceutical composition is administered by inhalation.

[0045] The liquid pharmaceutical composition is typically for use in the treatment or prevention of a disease or condition selected from asthma, allergic asthma, hay fever, allergic rhinitis, bronchitis, emphysema, bronchiectasis, chronic obstructive pulmonary disease (COPD), adult respiratory distress syndrome (ARDS), steroid-resistant asthma, severe asthma, childhood asthma, cystic fibrosis, lung fibrosis, pulmonary fibrosis, interstitial lung disease, skin disorders, atopic dermatitis, psoriasis, eye inflammation, cerebral ischemia, inflammatory disease and autoimmune disease.Preferably, the disease or condition is COPD or asthma.More preferably, the disease or condition is chronic obstructive pulmonary disease (COPD).

[0046] Typically, liquid pharmaceutical compositions are administered by inhalation at a metered nominal dose of 5 μg to 1000 μg per actuation from a pMDI containing the liquid pharmaceutical composition. For example, the metered nominal dose per actuation may be 10 μg to 500 μg. Typically, two actuations of a pMDI may be inhaled sequentially to provide a single dose.

[0047] Typically, the emitted dose from a pMDI containing a liquid pharmaceutical composition is 80% to 95% of the nominal metered dose.

[0048] Typically, liquid pharmaceutical compositions are administered to a patient via actuation of a pMDI two to eight times per day.

[0049] The present invention will now be described in more detail by the following examples. EXAMPLES

[0050] material and method The materials used are as follows: · API: Micronized RPL 554. The properties of Micronized RPL 554 are shown in Table 1 below.

[0051] [Table 1]

[0052] Propellants: HFA-134a and HFA-227ea Co-solvent: Ethyl alcohol / dehydrated alcohol (100% USP-NF ethanol) obtained from Hyman Kimia Surfactant: Oleic acid (Ph.Eur. / BP) obtained from Croda International plc. · Reagents: Analytical grade chemicals were used throughout the studies. Borosilicate glass vials with foil-lined polypropylene closures (VWR, part number 215-3905) Glass vials: 15ml pressure-rated clear glass vials (Neville & Moore, part number 0771C3 / A3) Valves: Bespak and Aptar 63μl valves Can: Presspart 14ml can Actuator: Presspart Actuator

[0053] All samples were prepared in the laboratory using a Pamasol hand crimper and filler. All samples were mixed by immersion in an ultrasonic bath for 30 seconds.

[0054] All analytical methods were fully developed and in vitro testing was performed using DUSA tubes and Next Generation Impaction (NGI).

[0055] The analytical test methods used during the analysis are as follows: Compendial requirements (European Pharmacopoeia (Ph.Eur) - Preparations for Inhalation (0671) and United States Pharmacopoeia <601> Delivered dose testing conducted in accordance with Chapter Compendial requirements (European Pharmacopoeia (Ph.Eur.) Method Chapter 2.9.18 and United States Pharmacopoeia <601> Next Generation Impactor (Next Generation Impactor) Aerodynamic Particle Size Distribution (APSD) testing performed using a 300-mm Impactor

[0056] Example 1 - Evaluation of suitable propellants Experiments were conducted to compare the suitability of two propellants, HFA-134a and HFA-227ea, for use as diluents in pMDI suspension formulations containing RPL 554. Table 2 shows the main physical and chemical properties of the two propellants evaluated.

[0057] [Table 2]

[0058] Samples were created by adding the required amount of RPL554 (see Table 3) to a 15 ml pressure-rated clear glass vial. The propellant, HFA-134a, HFA-227ea, or a combination of the two (see Table 4), was added through the valve and the samples were mixed in an ultrasonic bath for 30 seconds. The same target fill weight of 11.6 g (± 0.5 g) was used for all samples. The appearance of API particles in the propellant was evaluated in terms of both settling rate and aggregation behavior (see Figure 1). The settling rate of the formulation was visually assessed after suspension agitation by recording images of the glass vial using a high-speed camera. The quality of the suspension was determined by assigning a settling score of 1 to 10, with 1 being the fastest settling / cream (i.e., undesirable) and 10 being the slowest settling / cream (i.e., acceptable), based on the amount of active particles that settled or floated in 30 seconds.

[0059] [Table 3]

[0060] [Table 4]

[0061] result The RPL554 formulation in HFA-134a was easily suspended upon shaking, with rapid flocculation and slow settling observed after shaking was stopped. Formulations in HFA-227ea and a 50 / 50 blend of HFA-134a / HFA-227ea were also easily suspended upon shaking. However, creaming was observed after shaking was stopped for both HFA-227ea-containing formulations. The HFA-227ea formulation showed evidence of particle adhesion to the container walls after shaking, with more defined and larger flocs.

[0062] The sedimentation scores using each product strength propellant (pure or blended) are shown graphically in Figure 2, which shows that the best scores (i.e., slowest sedimentation) were observed for formulations with higher HFA-134a content. Sedimentation scores decreased as product strength increased due to increased powder loading. Table 5 shows the individual sedimentation scores.

[0063] [Table 5]

[0064] As can be seen from Table 5, formulations containing HFA-134a as a diluent were unexpectedly found to have consistently improved sedimentation scores compared to formulations containing HFA-227ea as a diluent. Formulations containing a higher percentage of HFA-134a were found to lead to less clumping, less creaming and less adhesion compared to formulations containing a higher percentage of HFA-227ea.

[0065] It has therefore been found that HFA-134a may be advantageously used as the sole diluent for a liquid pharmaceutical composition comprising a suspension of particles of RPL554.

[0066] Example 2 - Evaluation of suitable additional additives In an effort to improve the suspension properties, the inclusion of additional excipients was investigated. In particular, formulations were prepared that further contained a surfactant (oleic acid, Ph.Eur. / BP) and a co-solvent (ethanol, 100% USP-NF). The compositions of the formulations tested and the observed sedimentation scores are shown in Table 6.

[0067] [Table 6]

[0068] It was observed that the suspension scores of formulations containing oleic acid and ethanol were generally comparable to or lower than those of formulations without such excipients. However, in some samples, it was observed that RPL554 appeared to dissolve and recrystallize. To assess the solubility of RPL554 in additional excipients, a solubility analysis was performed.

[0069] Example 3 - Solubility of RPL554 in Ethanol RPL554 was originally deemed to be substantially insoluble in ethanol according to the USP / BP solubility criteria shown in Table 7. However, the observation of dissolution and recrystallization at the lowest product strength (10 μg / actuation) prompted further investigation of the solubility of RPL554 in ethanol in the presence of oleic acid.

[0070] [Table 7]

[0071] Experimental Method Samples were prepared by adding 1.5 mg of RPL554 to a plain 14 mL aluminum storage container with ethanol, oleic acid, and HFA-134a. Excipients were added to the storage container to maintain a 50:1 ethanol / oleic acid ratio (see Table 8). A corresponding storage container containing only RPL554, ethanol, and HFA-134a was also produced. The storage container was crimped with a 63 μL pMDI valve with a shortened dip tube (Figure 3) and filled to 11.6 g with HFA-134a through the valve. The length of the dip tube was sufficient to extend only into the liquid propellant / co-solvent phase (i.e., after the API particles had settled). The sample was placed in an ultrasonic bath for 30 seconds.

[0072] [Table 8]

[0073] All storage vessels were left at ambient temperature for 24 hours to allow settling and establish dissolution equilibrium. To measure the amount of dissolved API, a 10.0 cm cannula was inserted (through a cannula attached to the valve stem) in an upright position from each storage vessel. 3 The RPL554 concentration was quantitatively calculated against a three-point calibration curve.

[0074] result The amount of dissolved RPL554 increased with increasing ethanol concentration. The presence of oleic acid in the formulation did not appear to affect the solubility of RPL554 (Figure 4).

[0075] A previous evaluation of RPL554's solubility in ethanol led to a classification of "practically insoluble" according to USP / BP criteria. The original evaluation was performed using analytical reagent grade ethanol. This time, the solubility was re-evaluated using dehydrated alcohol (100% USP-NF ethanol, i.e., the grade of ethanol typically used in the manufacture of bulk pMDI HFA formulation suspensions), and the solubility of RPL554 in ethanol was found to be approximately 1 part in 2000, which is "very slightly soluble" according to USP / BP criteria. Thus, unexpectedly, RPL554 was found to have some solubility in ethanol.

[0076] For suspension formulations, the solubility of the API in the co-solvent / propellant mixture should be minimal to avoid potential issues with chemical and physical instability. Any benefits associated with the use of oleic acid and ethanol as excipients were found to be largely outweighed by the dissolution of RPL554 and the vulnerability of RPL554 particles to Ostwald ripening. Thus, it was established that the most promising pMDI formulation of RPL554 is a suspension formulation of RPL554 in HFA-134a only.

[0077] Example 4 - Validation of HFA-134a-only formulation Further evaluation of HFA-134a-only formulations confirmed that RPL554 was adequately suspended for delivery via pMDI by preparing samples in pressure-rated glass vials. Three product strengths (50, 150 and 500 μg / actuation) were selected. Visual evaluation of these formulations demonstrated comparable suspension quality and settling rates to the commercially available HFA-134a-only pMDI product.

[0078] Product performance attributes of the RPL554 HFA-134a-only suspension-based formulation were further evaluated by preparing laboratory samples in 14mL plain aluminum storage containers of three strengths (50, 150 and 500μg / actuation) crimped with an Aptar 63μL valve. Samples were mixed in an ultrasonic bath for 30 seconds, then stored and inverted for 4 days before being analyzed for delivered dose uniformity (DDU) and aerodynamic particle size distribution (APSD) by next generation impaction (NGI).

[0079] Delivered Dose Uniformity (DDU) Delivered dose uniformity analysis was performed three times on three samples of each product strength at the beginning, middle and end of the shelf life. The average delivered dose results are shown in Table 9.

[0080] Overall, the average delivered dose was close to the expected target label claim for each product strength.

[0081] Shot Weight Shot weights were measured at the beginning, middle and end of the storage container's life to assess product performance over its life.

[0082] The shot weight of the laboratory prepared samples was found to be consistent across all samples with a mean value of 80.35 ± 0.54 mg (RSD = 0.67%, n = 9). The theoretical shot weight of 63 μL of HFA-134a is 77.2 mg.

[0083] Aerodynamic Particle Size Distribution (APSD) Aerodynamic particle size distribution was measured to determine the fine particle fraction (fine particle fraction, % dose at particle size less than 5 μm) of the tested formulations. The results are shown in Table 9.

[0084] [Table 9]

[0085] The correlation between the determined fine particle dose (FPD) and product strength is shown in Figure 5. Dose proportionality was observed.

[0086] Example 5 - Stability of formulations Stability studies were performed on all RPL554 formulation strengths. Example data for RPL554 HFA-134a pMDI, 500 μg / working product are shown in Tables 10 and 11 for stability studies performed at 25° C. / 60 and 40° C. / 75% RH, respectively. The data demonstrate consistent performance over a 6 month stability period.

[0087] [Table 10]

[0088] [Table 11]

Claims

1. A liquid pharmaceutical composition for use in the treatment or prevention of asthma, said composition being suitable for administration by inhalation, (i) a suspension of particles comprising 9,10-dimethoxy-2-(2,4,6-trimethylphenylimino)-3-(N-carbamoyl-2-aminoethyl)-3,4,6,7-tetrahydro-2H-pyrimido[6,1-a]isoquinolin-4-one (RPL554); and (ii) a diluent which is 1,1,1,2-tetrafluoroethane (HFA-134a) wherein said liquid pharmaceutical composition contains less than 0.05% by weight of a surfactant based on the total weight of the composition.

2. A liquid pharmaceutical composition for use as described in claim 1, wherein the liquid pharmaceutical composition contains less than 0.05 weight % of any additional excipients selected from co-solvents and surfactants relative to the total weight of the composition.

3. A liquid pharmaceutical composition for use as described in claim 1, wherein the liquid pharmaceutical composition contains less than 0.05 weight % of any additional excipients based on the total weight of the composition.

4. A liquid pharmaceutical composition for use as described in claim 1, wherein the liquid pharmaceutical composition contains less than 0.01 weight % of said surfactant or said additional excipient relative to the total weight of the composition.

5. A liquid pharmaceutical composition for use as described in claim 1, wherein the liquid pharmaceutical composition contains less than 0.001% by weight of said surfactant or said additional excipient relative to the total weight of the composition.

6. The liquid pharmaceutical composition for use according to claim 1, wherein the particles comprise at least 50% by weight of RPL554 relative to the total weight of the particles.

7. The liquid pharmaceutical composition for use according to claim 1, wherein the particles contain at least 99% by weight of RPL554 based on the total weight of the particles.

8. The liquid pharmaceutical composition for use according to claim 1, wherein RPL554 is the only active agent.

9. A liquid pharmaceutical composition for use according to claim 1, consisting essentially of (i) RPL554 and (ii) 1,1,1,2-tetrafluoroethane.

10. A liquid pharmaceutical composition for use according to claim 1, comprising at least 99.5% by weight of (i) RPL554 and (ii) 1,1,1,2-tetrafluoroethane based on the total weight of the composition.

11. The liquid pharmaceutical composition for use according to claim 1, wherein the particles comprising RPL554 have a particle size distribution with a Dv50 (median particle size by volume) value of 0.2 μm to 5 μm.

12. The liquid pharmaceutical composition for use according to claim 1, wherein the concentration of the particles comprising RPL554 in the liquid pharmaceutical composition is from 0.1 mg / mL to 200 mg / mL.

13. A liquid pharmaceutical composition for use as described in claim 1, suitable for administration by a pressurized metered dose inhaler.

14. A liquid pharmaceutical composition for use as described in claim 1, wherein the liquid pharmaceutical composition is in a pressurized metered dose inhaler.

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