Cosmetic method and cosmetic device using ultrasonic wave

Abstract

To provide a novel cosmetic method and a cosmetic device for use for the cosmetic method.SOLUTION: A cosmetic method comprises a step for applying an ultrasonic wave to a skin so as to promote production of collagen, hyaluronic acid and / or elastin being skin cells. There is also provided an ultrasonic wave cosmetic device used for the cosmetic method. The ultrasonic wave cosmetic device comprises a transducer which radiates an ultrasonic wave configured to apply an ultrasonic wave to a skin by contacting the skin, where the frequency of the radiated ultrasonic wave can be adjusted to 0.1 to 10 MHz, and output can be adjusted to a range of 0.5 to 2 W.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a beauty method using ultrasonic waves, more specifically, a beauty method including applying ultrasonic waves to the skin to promote the production of collagen, hyaluronic acid and / or elastin in skin cells, a beauty device for use in such a beauty method, and an operating method thereof.

Background Art

[0002] Fibroblasts present in the dermis of the skin produce components such as collagen, elastin, and hyaluronic acid. Collagen is an essential component for maintaining the elasticity of the skin by forming a network structure. However, due to aging, ultraviolet rays, stress, etc., when the production amount of collagen decreases, it causes a decrease in skin elasticity, wrinkles, and sagging.

[0003] Many methods have been proposed to promote the production of such components for the purpose of beauty improvement. Typical examples include drug administration of production promoters of these components, etc. In addition, there has been proposed a beauty treatment device that promotes the regeneration of collagen in the dermis by applying a high-frequency (RF) current to the skin of the face, hands, feet, etc. to cause thermal damage to the collagen in the dermis (for example, Patent Document 1).

[0004] There has also been proposed a high-intensity focused ultrasound therapy (HIFU) for beauty medicine that applies ultrasonic waves to the skin instead of RF current and uses the thermal effect of ultrasonic waves to improve skin sagging and wrinkles (Non-Patent Document 1). HIFU is a technique that concentrates and irradiates ultrasonic waves at a single point and acts only on the targeted part without damaging the skin surface and surrounding tissues. Originally, it uses strong thermal energy to destroy cancer cells and is used for the treatment of prostate cancer, but it is also used for beauty purposes by reducing the output. The principle is that the vibration energy of ultrasonic waves is absorbed by components such as collagen in the living body and converted into thermal energy to exert a thermal effect. The absorption rate of ultrasonic energy is proportional to the content of components such as collagen in the tissue, and it is said that the thermal effect is high in tissues with a large amount.

[0005] There is no knowledge about how the use of ultrasonic irradiation for cosmetic purposes affects the content and production of skin components.

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

Non-Patent Documents

[0007]

Non-Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0008] An object of the present invention is to provide a new cosmetic method and a cosmetic device for use in such a cosmetic method.

Means for Solving the Problems

[0009] The present inventor examined how the use of ultrasonic irradiation for cosmetic purposes affects the content and production of skin components, and surprisingly found that the expression and production of components such as collagen, hyaluronic acid, and elastin, which are essential for the skin to maintain elasticity, are promoted by fibroblasts.

[0010] Therefore, the present application includes the following inventions. (1) A cosmetic method comprising applying ultrasonic waves to the skin to promote the production of collagen, hyaluronic acid, and / or elastin in skin cells. (2) The cosmetic method according to (1), wherein the frequency of the ultrasonic waves is 0.1 to 10 MHz and the output is 0.5 to 2 W. (3) The cosmetic method according to (2), wherein the frequency of the ultrasonic waves is 1 to 5 MHz and the output is 0.7 to 1.5 W. (4) The beauty method according to (1), wherein the frequency of the ultrasonic wave is 4 to 6 MHz, the output is 1.5 to 2 W, and the promotion of the production of collagen, hyaluronic acid and / or elastin is maintained for at least 3 days. (5) The beauty method according to (1), wherein the frequency of the ultrasonic wave is 2 to 4 MHz, the output is 0.7 to 1.5 W, and the promotion of the production of hyaluronic acid and / or elastin is maintained for at least 3 days. (6) An operating method of an ultrasonic beauty device including a transducer that emits ultrasonic waves, which is operated to apply ultrasonic waves to the skin with the transducer in contact with the skin surface, thereby promoting the production of collagen, hyaluronic acid and / or elastin in skin cells. An operating method of an ultrasonic beauty device. (7) The operating method of the ultrasonic beauty device according to (6), wherein the frequency of the ultrasonic wave is 0.1 to 10 MHz and the output is 0.5 to 2 W. (8) The operating method of the ultrasonic beauty device according to (7), wherein the frequency of the ultrasonic wave is 1 to 5 MHz and the output is 0.7 to 1.5 W. (9) The operating method of the ultrasonic beauty device according to (6), wherein the frequency of the ultrasonic wave is 4 to 6 MHz, the output is 1.5 to 2 W, and the promotion of the production of collagen, hyaluronic acid and / or elastin is maintained for at least 3 days. (10) The operating method of the ultrasonic beauty device according to (6), wherein the frequency of the ultrasonic wave is 2 to 4 MHz, the output is 0.7 to 1.5 W, and the promotion of the production of hyaluronic acid and / or elastin is maintained for at least 3 days. (11) An ultrasonic beauty device that promotes the production of collagen, hyaluronic acid and / or elastin in skin cells by applying ultrasonic waves to the skin, comprising a transducer that emits ultrasonic waves and is configured to apply ultrasonic waves to the skin by contacting the skin, characterized in that the frequency of the emitted ultrasonic wave is adjustable within the range of 0.1 to 10 MHz and the output is 0.5 to 2 W. (12) The ultrasonic beauty device according to (11), wherein the frequency of the ultrasonic wave is adjustable to 1 to 5 MHz and the output is 0.7 to 1.5 W. (13) An ultrasonic beauty device according to (11), further comprising a timer for setting the application time to the skin to be 1 to 10 minutes. (14) An ultrasonic beauty device according to (11), wherein the frequency of the ultrasonic wave is adjustable to 4 to 6 MHz and the output is adjustable to 1.5 to 2 W, and the promotion of the production of collagen, hyaluronic acid and / or elastin is maintained for at least 3 days. (15) An ultrasonic beauty device according to (11), wherein the frequency of the ultrasonic wave is adjustable to 2 to 4 MHz and the output is adjustable to 0.7 to 1.5 W, and the promotion of the production of hyaluronic acid and / or elastin is maintained for at least 3 days. [Effects of the Invention]

[0011] By the beauty method and the ultrasonic beauty device of the present invention, the production of collagen, hyaluronic acid and / or elastin in skin cells is promoted, and as a result, prevention and improvement of skin and muscle wrinkles and sagging are achieved. [Brief Description of the Drawings]

[0012]

Figure 1

Figure 2

Figure 3

Figure 4

[0013] The present invention is based on the new findings of the present inventors that the application of ultrasonic waves to the skin can promote the expression and production of collagen, hyaluronic acid and elastin by fibroblasts.

[0014] One of the factors of wrinkles and sagging is due to the thinning of the skin tissue with aging. In aged skin, the reduction of collagen fibers, which are the main matrix components of the dermis, is remarkable, and this is the main cause of the reduction in the thickness of the skin. Therefore, it is considered effective to promote the production of collagen and maintain the amount of collagen for the prevention and improvement of wrinkles and sagging.

[0015] Hyaluronic acid has many functions, such as retaining moisture in the intercellular space, retaining cells based on forming a jelly-like matrix in the tissue, maintaining the lubricity and flexibility of the tissue, resisting external forces such as mechanical damage, and preventing bacterial infection. For example, it is said that the amount of hyaluronic acid in the skin decreases with aging, and accordingly, skin aging such as fine wrinkles and roughness appears. Therefore, many cosmetics containing hyaluronic acid have been proposed as improving agents for such aged skin.

[0016] Elastin is the most important component of elastic fibers. Elastic fibers play an important role in the elasticity of the skin, and the reduction and degeneration of elastic fibers are considered to be one of the causes of sagging and wrinkles associated with aging. Elastic fibers are formed by tropoelastin, which is a molecule of elastin, aggregating and arranging on microfibrils formed by polymerization of a number of elastic fiber components with fibrillin-1 as the main component, and further cross-linking between molecules by lysyl oxidase to form insoluble fibers, giving the skin firmness and elasticity. It has been proposed to increase elastin expression at a normal level and promote the reconstruction of elastic fibers for the improvement of sagging and wrinkles.

[0017] As described above, collagen, hyaluronic acid, and elastin are essential components for maintaining the elasticity of the skin. The inventor has found that the application of ultrasonic waves to the skin promotes the expression and production of collagen, hyaluronic acid, and / or elastin by fibroblasts, thereby enhancing the elasticity of the skin and ultimately improving wrinkles and sagging, leading to the completion of the present invention.

[0018] The ultrasonic beauty device used in the beauty method of the present invention includes a main body that generates ultrasonic waves and a transducer that emits ultrasonic waves of a predetermined frequency at a predetermined output to the skin surface to be beautified. This ultrasonic beauty device can be configured to oscillate ultrasonic waves with a variable frequency that continuously or intermittently changes at predetermined intervals in a predetermined frequency band against the skin surface to be treated. Alternatively, this ultrasonic wave generating device can be configured to oscillate ultrasonic waves with a variable frequency that continuously or intermittently changes at predetermined intervals in a predetermined frequency band against the skin surface to be treated.

[0019] As the ultrasonic beauty device of the present invention, one that can generate ultrasonic waves in the frequency range of 0.1 to 10 MHz, preferably 1 to 5 MHz, and whose output can be adjusted between 0.5 and 2 W, preferably 0.7 and 1.5 W, is used. This is because it is preferable to appropriately adjust the frequency and output within the above ranges for the purpose of promoting the production of collagen, hyaluronic acid, and / or elastin and maintaining the promotion of their production.

[0020] In another aspect, as the ultrasonic beauty device of the invention, one that can generate ultrasonic waves in the frequency range of 4 to 6 MHz and whose output can be adjusted between 1.5 and 2 W is used. This is because by adjusting the frequency and output within the above range, the promotion of the production of collagen, hyaluronic acid, and / or elastin can be maintained for at least three days.

[0021] In yet another aspect, as the ultrasonic beauty device of the invention, one that can generate ultrasonic waves with a frequency between 2 and 4 MHz and whose output can be adjusted between 0.7 and 1.5 W at that time is used. By adjusting the frequency and output within the above ranges, it is possible to maintain the promotion of the production of hyaluronic acid and / or elastin for at least 3 days.

[0022] The beauty ultrasonic device used in the present invention is designed such that ultrasonic waves are propagated in the skin from the skin surface to a maximum depth of 1 to 10 mm, preferably 0.2 to 1.8 mm, thereby stimulating skin cells and enabling the promotion of the production of collagen, hyaluronic acid and / or elastin and the maintenance of such promotion of production.

[0023] The time for radiating ultrasonic waves onto the skin surface is not particularly limited, but it may be 10 seconds to 30 minutes, preferably 30 seconds to 15 minutes, more preferably 1 minute to 10 minutes for the promotion of the production of collagen, hyaluronic acid and / or elastin and / or the maintenance thereof. The ultrasonic beauty device of the present invention may be provided with a timer for setting the radiation time within the above ranges.

[0024] "Promotion of production" as referred to in the present invention means that, for example, compared with a subject to which ultrasonic waves having the frequency and frequencies defined in the present invention are not applied, the production of each of collagen, hyaluronic acid and elastin increases by, for example, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, 100% or more, 200% or more, 300% or more, 400% or more, or 500% or more at the protein level or the gene level.

[0025] The application of ultrasonic waves to the skin may be combined with the application of drugs, various extracts, etc. to the skin. For example, applicable drugs and various extracts include inositol, retinol derivatives, xylitol, hyaluronic acid, glycerin, yeast extract, sakurareef extract, cinnamon extract, pine extract, Bulgarian rose water, okra extract, ibuki jakou extract, waremokou extract, dipotassium glycyrrhizinate, ginkgo leaf extract, tencha extract, yokukinin extract, licorice extract, carrot extract, clara extract, star fruit leaf extract, carrot extract, dioscorea extract, neem leaf extract, saffron extract, cinchona extract, comfrey extract, thyme extract, winter strawberry, wild rocket, mube, Okinawa strawberry, Indian wild rocket, matebashii, gold extract, rosemary extract, apricot kernel, gentiana extract, mint powder, taisou extract, hop extract, kiwi extract, Roman chamomile extract, apple extract, Japanese quince extract, turmeric extract, yam, peach, oak, etc. These can be used alone or in combinations of multiple.

[0026] All documents mentioned in this specification are hereby incorporated by reference in their entirety.

[0027] The embodiments of the present invention described below are for illustrative purposes only and do not limit the technical scope of the present invention. The technical scope of the present invention is limited only by the description in the claims. Changes to the present invention, for example, addition, deletion, and substitution of the constituent elements of the present invention, can be made on the condition that the gist of the present invention is not deviated from.

Examples

[0028] The present invention will be described in more detail below based on examples, but these do not limit the present invention in any way.

[0029] Ultrasonic irradiation method For ultrasonic irradiation of cells, a device that generates ultrasonic waves of 1 to 5 MHz was used. The duty ratio of the output was set to 100%, and the ultrasonic power applied was 0.9 W (LOW) or 1.8 W (HIGH).

[0030] For ultrasonic irradiation of humans, a beauty device (KRD-1007N, Beautiful Angel Co., Ltd.) that generates ultrasonic waves of 3 MHz was used. The duty ratio of the output was set to 100%, and the maximum ultrasonic power applied was 0.74 W.

[0031] Gene expression analysis of COL1A2, ELN, and HAS2 in fibroblasts The purchased normal human dermal fibroblasts (cat. no. KF-4009, lot no. 02017, KURABO) were seeded in a 100 mm culture dish (cat. no. 353003, CORNING), pre-cultured in DMEM medium (cat. no. 11885084, Invitrogen) containing 10% FBS, and then the fibroblasts suspension prepared by detaching the cells according to the conventional method was stored in a polyester film. The medium used was also DMEM medium containing 10% FBS. An appropriate amount of degassed water was added to a container with a sound-absorbing material at the bottom, and ultrasonic irradiation was performed so that the polyester film storing the fibroblasts suspension was at a certain distance from the ultrasonic vibrator. After ultrasonic irradiation, the cell suspension was recovered from the polyester film and seeded into plastic petri dishes (cat. no. 3506, CORNING) prepared for the non-ultrasonic irradiation group and the 2-minute ultrasonic irradiation group. The non-ultrasonic irradiation group was used as a control group that was exposed to degassed water after being stored in the polyester film. The cells were cultured in a CO2 incubator for 2 days and 3 days, and then total RNA was recovered using the RNeasy Mini Kit (cat. no. 74106, Qiagen). The RNA concentration was measured with NanoDrop, and cDNA was synthesized from 1 μg of RNA using the SuperScript VILO cDNA Synthesis Kit (cat. no. 11754, Invitrogen). Quantitative analysis of the gene expression levels of COL1A2 (type I collagen), ELN (elastin), and HAS2 (hyaluronan synthase) was performed using SYBR Green (cat. no. 11733046, Invitrogen) with StepOne (Applied Bioscience). The primers used in the reaction are shown in Tables 1 - 3. GAPDH was used as the housekeeping gene.

[0032]

Table 1

[0033]

Table 2

[0034]

Table 3

[0035] Figure 1 shows the test results when fibroblasts were irradiated with ultrasonic waves in the frequency range of 1 - 5 MHz and output range of 0.9 - 1.8 W for 2 minutes. It can be seen that within the test range of ultrasonic wave frequency from 1 - 5 MHz and output from 0.9 - 1.8 W, the production capacity of collagen, elastin, and hyaluronic acid by fibroblasts increases significantly.

[0036] Therefore, it was found that by irradiating fibroblasts with ultrasonic waves of a predetermined frequency and output, the production of collagen, elastin, and hyaluronic acid can be enhanced.

[0037] Figure 2 shows the results of examining whether the enhanced expression of each gene is maintained over several days when fibroblasts were irradiated with ultrasonic waves with the frequency fixed at 5 MHz and the output set at 0.9 W (Low: low output) and 1.8 W (High: high output). At low output, the enhanced expression of all three genes was maintained until the second day, but on the third day, the enhanced expression of all three genes was not observed. On the other hand, in the case of high output, the enhanced expression of all three genes was maintained on both the second day and the third day.

[0038] Figure 3 shows the results of examining whether the enhanced expression of each gene is maintained over several days when fibroblasts were irradiated with ultrasonic waves with the frequency fixed at 3 MHz and the output set at 0.9 W (low output) and 1.8 W (high output). In the case of a frequency of 3 MHz, for both low output and high output, the enhanced expression of elastin and hyaluronic acid was maintained on both the second day and the third day.

[0039] Examination of the effect of ultrasonic waves on sagging improvement in humans Based on the Helsinki Declaration, the selection of volunteers followed the medical and ethical standards of human examinations, and informed consent was obtained before the examinations. All procedures involving humans were approved by the Institutional Review Board of the Shiseido Global Innovation Center. Twelve Asians were registered as volunteers. The serum used simultaneously with the beauty device was utilized only for the purpose of improving the usability of the ultrasonic beauty device and did not contain specific compounds. An ultrasonic beauty device with a frequency of 3 MHz and a maximum output of 0.74 W was used. In the morning, the serum was evenly applied to the face, and in the evening, the ultrasonic beauty device was used for 3 minutes on one side and a total of 6 minutes for the entire face. It was used continuously for 4 weeks, and visual evaluation of the jaw line and sagging at the corners of the mouth and subjective evaluation by the subjects' questionnaires were conducted before continuous use and at 1, 2, and 4 weeks of continuous use. The visual evaluation was scored according to the following criteria: 0: No sagging is observed 1: Slight sagging is observed 2: Sagging is observed 3: Obvious sagging is observed 4: Very obvious sagging is observed The subjective evaluation by the subjects' questionnaires was scored according to the following criteria: -4: Very bad -3: Bad -2: Slightly bad -1: Rather bad 0: Normal 1: Rather good 2: Slightly good 3: Good 4: Very good The results are shown in Figure 4. In both the jaw line and the corners of the mouth, an improvement effect on sagging (tightening) by ultrasound was recognized in the visual evaluation and the subjects' questionnaires.

Claims

1. A beauty method comprising applying ultrasonic waves to the skin to promote the production of collagen, hyaluronic acid and / or elastin in skin cells.

2. The beauty method according to claim 1, wherein the frequency of the ultrasonic waves is 0.1 to 10 MHz and the output is 0.5 to 2 W.

3. The beauty method according to claim 2, wherein the frequency of the ultrasonic waves is 1 to 5 MHz and the output is 0.7 to 1.5 W.

4. The beauty method according to claim 1, wherein the frequency of the ultrasonic waves is 4 to 6 MHz, the output is 1.5 to 2 W, and the promotion of the production of collagen, hyaluronic acid and / or elastin is maintained for at least 3 days.

5. The beauty method according to claim 1, wherein the frequency of the ultrasonic waves is 2 to 4 MHz, the output is 0.7 to 1.5 W, and the promotion of the production of hyaluronic acid and / or elastin is maintained for at least 3 days.

6. An operating method of an ultrasonic beauty device equipped with a transducer that emits ultrasonic waves, the method being configured to operate so as to apply ultrasonic waves to the skin with the transducer in contact with the skin surface, thereby promoting the production of collagen, hyaluronic acid and / or elastin in skin cells. Operating method of an ultrasonic beauty device.

7. The operating method of the ultrasonic beauty device according to claim 6, wherein the frequency of the ultrasonic waves is 0.1 to 10 MHz and the output is 0.5 to 2 W.

8. The operating method of the ultrasonic beauty device according to claim 7, wherein the frequency of the ultrasonic waves is 1 to 5 MHz and the output is 0.7 to 1.5 W.

9. The operating method of the ultrasonic beauty device according to claim 6, wherein the frequency of the ultrasonic waves is 4 to 6 MHz, the output is 1.5 to 2 W, and the promotion of the production of collagen, hyaluronic acid and / or elastin is maintained for at least 3 days.

10. The operating method of the ultrasonic beauty device according to claim 6, wherein the frequency of the ultrasonic waves is 2 to 4 MHz, the output is 0.7 to 1.5 W, and the promotion of the production of hyaluronic acid and / or elastin is maintained for at least 3 days.

11. An ultrasonic beauty device that promotes the production of collagen, hyaluronic acid and / or elastin in skin cells by applying ultrasonic waves to the skin, comprising a transducer that emits ultrasonic waves and is configured to apply ultrasonic waves to the skin by contacting the skin. An ultrasonic beauty device, characterized in that the frequency of the radiated ultrasonic wave can be adjusted within the range of 0.1 to 10 MHz and the output can be adjusted within the range of 0.5 to 2 W.

12. The ultrasonic beauty device according to claim 11, wherein the frequency of the ultrasonic wave can be adjusted to 1 to 5 MHz and the output can be adjusted to 0.7 to 1.5 W.

13. The ultrasonic beauty device according to claim 11, further comprising a timer for setting the application time to the skin to 1 to 10 minutes.

14. The ultrasonic beauty device according to claim 11, wherein the frequency of the ultrasonic wave can be adjusted to 4 to 6 MHz and the output can be adjusted to 1.5 to 2 W, and the promotion of the production of collagen, hyaluronic acid and / or elastin is maintained for at least 3 days.

15. The ultrasonic beauty device according to claim 11, wherein the frequency of the ultrasonic wave can be adjusted to 2 to 4 MHz and the output can be adjusted to 0.7 to 1.5 W, and the promotion of the production of hyaluronic acid and / or elastin is maintained for at least 3 days.

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