Extracts from ginger plants, compositions thereof, and their use in pest control.

Abstract

This invention provides the use of ginger plant extracts, insecticidal compositions, and methods of use that have excellent anti-insect activity against pests such as mites and thrips. [Solution] The present invention provides the use of ginger family plant extracts in the killing, control, extermination, prevention or reduction of pest oviposition, and prevention or reduction of pest egg hatching, wherein the ginger family plant is one or more selected from ginger, turmeric, galangal, hepatica, and ginger lily, the extract can be obtained by extracting from one ginger family plant or from two or more ginger family plants, and the pest is selected from thrips, spider mites, lepidae, gall mites, dust mites, lice, green mites, predatory mites, scabiidae, dust mites, and dust mites.

Description

Technical Field

[0001] The present invention mainly relates to the use in pest control of Zingiberaceae plants and the insecticidal composition of extracts of Zingiberaceae plants, and includes the fields of natural plant chemistry and pest control.

Background Art

[0002] Pest control is a global problem. At present, hundreds of chemically developed repellents, growth regulators, and insecticides such as pyrethroids, DEET and other aromatic amides, and organophosphates have emerged and are widely used. The effectiveness of these products is often limited by factors such as toxicity to the human body or environment, insect drug resistance (especially resistance to pyrethroids, see Romero et al.), limited dry residue activity, repellency, and physical factors (odor, coloring) not suitable for indoor use. However, in recent years, the demand for effective pest control products of natural origin has been very high.

[0003] Mites are significant pests in agricultural production, damaging cotton, grains, fruit trees, forest trees, and ornamental plants. The striped spider mite is one of the top 10 major pests in China. The two-spotted spider mite can penetrate 18-22 plant cells per minute. Apple spider mites and hawthorn spider mites are significant pests of fruit trees in northern China, and severe damage can reduce fruit yield by 1 / 3 to 2 / 3. The sweet potato mite is present in wheat-producing areas throughout the country, and in severe cases, wheat grains may not be harvested at all. Many types of spider mites infest cotton fields, reducing yields by more than 30% and sometimes completely burning the cotton plants. Species of the fern subfamily attack grasses and shrubs. The alfalfa mite is a species distributed worldwide and often damages wheat crops simultaneously with the sweet potato mite in northern China. The false clover spider mite is distributed in America, Europe, Asia, South Africa, and Oceania and is a major pest of fruit trees. In northern China, 3 to 5 generations occur per year, while in the south, 8 to 10 generations occur. The subfamily Mitininae infests all higher plants. Commonly found genera include *Tetranychus*, *Pannonius*, *Eotetranicus*, *Oligonychus*, and *Tetranychus*. Among these, the citrus spider mite is widely distributed in citrus-producing regions worldwide and can damage both citrus seedlings and mature trees, causing grayish-white spots on the leaves. The juniper spider mite damages cypress trees and is distributed in China and Japan. Cypress trees suffer severe damage in drought years, with yellowing of the canopy and loss of needles. Schizotetranicus yoshimekii is distributed in southwestern China and Thailand, and causes serious damage to rice plants. It sucks the leaves, causing the entire leaf to lose its green color and appear grayish-green or grayish-white. Affected rice plants have shorter ears and smaller grains, generally resulting in a yield reduction of about 10%, and in severe cases, it can reduce yield by more than 30%.

[0004] Thrips are insects belonging to the order Thysanoptera. They are very small and typically damage flowers and leaves. Due to their high reproductive rate, multiple generations, and high incidence, thrips are gradually spreading and causing damage due to global trade and climate warming, making them one of the most important agricultural pests in the world. Thrips damage a variety of plants in agriculture, forestry, and horticulture, but the damage is most severe in tropical and subtropical regions. The rapid spread of thrips is further exacerbated by the transportation of agricultural products from various regions. The citrus thrips and the western flower thrips are severely endemic worldwide. Globally or regionally important thrips pests include the onion thrips (Thrips tabaci Lindeman), the flower thrips (Thrips hawaiiensis (Morgan)), the flat-headed flower thrips (Frankliniella intonsa (Trybom)), the western flower thrips (Frankliniella bispinosa (Morgan)), and the tea thrips (Scirtothrips dorsalis Hood).

[0005] Ginger is a perennial plant known for its distinctive spiciness and wide range of medicinal properties. Native to Southeast Asia, ginger is now widely cultivated in tropical and subtropical regions worldwide and is one of the important economic crops. There are many varieties of ginger, including the common Zingiber officinale, as well as turmeric (Curcuma longa), Kaempferia galanga, Alpinia galanga, Hedychium coronarium, and Alpinia zerumbet.

[0006] The uses of ginger in agriculture are not limited to the cultivation of seasonings and medicinal ingredients; its extracts show potential for various applications in modern agriculture. Extracts from ginger plants are rich in active ingredients such as gingerol, zingiberone, gingerol, pinene, camphene, α-terpineol, and linalool. Extracts from ginger plants possess various biological activities, including antibacterial, antifungal, antiviral, and insecticidal properties. The insecticidal effect is mainly concentrated on parasites, coleoptera such as drugstore beetles, hemipterans such as whiteflies, lepidoptera such as beet armyworms, diptera such as fruit flies, and hymenoptera such as alfalfa leafcutter wasps, but no effect against pests such as mites and thrips has been reported. [Overview of the project]

[0007] In order to provide natural insecticides that have good control effects against mites, thrips, and other pests, the present invention intends to conduct research on ginger plants with the expectation of discovering new opportunities for controlling mites, thrips, and other pests.

[0008] This invention is the first to demonstrate that extracts from ginger plants possess excellent anti-insect activity against pests such as mites and thrips. Furthermore, the synergistic relationship between extracts from different species of ginger plants expands the range of applications for ginger plant extracts and provides a new option for controlling mites and thrips in agriculture.

[0009] Furthermore, in several embodiments, the present invention has shown that ginger plant extracts can significantly control targets such as false spider mites, citrus red mites, two-spotted spider mites, and thrips, and exhibit contact toxicity and culling toxicity indicators against nymphs, adults, and eggs.

[0010] Specifically, according to the present invention, the use of ginger plant extracts in the killing and / or control of pests, and / or extermination of pests, and / or prevention or reduction of pest oviposition, and / or prevention or reduction of hatching of pest eggs, wherein the ginger plant is one or more selected from ginger, turmeric, galangal, hepatica, and ginger lily, and the extract is one or more selected from volatile oil, water extract, ethanol extract, ethyl acetate extract, and methanol extract, and The exudate can be obtained by extracting from one species of Zingiberaceae plant or from two or more species of Zingiberaceae plants, and the pests provided are selected from insects of the family Thripsidae, and / or spider mites, and / or gall mites, and / or dust mites, and / or lice mites, and / or green mites, and / or predatory mites, and / or scabiidae, and / or dust mites.

[0011] During extraction, various parts of the ginger family plant can be used, including, but not limited to, the stems and above-ground parts.

[0012] Furthermore, the ginger plant extract is selected from ginger rhizome extract and turmeric rhizome extract, or from a mixture of ginger rhizome extract and turmeric rhizome extract in a ratio of 1-9:9-1. For example, the composition may have a blending ratio of ginger rhizome extract to turmeric rhizome extract of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 2:1, 2:3, 2:5, 2:7, 2:9, 3:1, 3:2, 3:4, 3:5, 3:7, 3:8, 4:1, 4:3, 4:5, 4:7, 4:9, 5:1, 5:2, 5:3, 5:4, 5:6, 5:7, 5:8, 5:9, 6:1, 6:5, 6:7, 7:1, 7:2, 7:3, 7:4, 7:5, 7:6, 7:8, 7:9, 8:1, 8:3, 8:5, 8:7, 8:9, 9:1, 9:2, 9:4, 9:5, 9:7, 9:8, etc.

[0013] Furthermore, the mixing ratio of ginger rhizome extract to turmeric rhizome extract is 7-9:3-1, for example, 7:3, 7:2, 7:1, 8:3, 8:2, 8:1, 9:3, 9:2, 9:1, etc.

[0014] Furthermore, the ginger rhizome extract is extracted from ginger rhizomes using ethanol:ethyl acetate in a ratio of 1 to 4:1, and the turmeric rhizome extract is volatile oil from turmeric rhizomes. Research has shown that when the extract is selected from the above, the insect pest control effect is good. Moreover, in this invention, when the mixing ratio of ginger rhizome extract to turmeric rhizome extract is 7:3, a synergistic effect can be achieved, and the insect pest control effect is significantly improved.

[0015] In the present invention, the extraction method for water extracts, ethanol extracts, ethyl acetate extracts, etc., can be any conventional plant extraction method. For example, heat extraction, diafiltration extraction, ultrasonic extraction, microwave extraction, immersion extraction, supercritical fluid extraction, etc., can be used, but are not limited to these.

[0016] The "ethanol" of this invention is selected from ethanol with a concentration of 95% or higher, and includes, but is not limited to, 95% ethanol, anhydrous ethanol, etc.

[0017] In some embodiments of the present invention, extraction is preferably performed using a mixed solvent of ethanol and ethyl acetate. Studies have shown that the pest control effect of mixed solvent extracts is superior to that of single solvents. The mixed solvent may be ethanol:ethyl acetate = 1 to 4:1.

[0018] In embodiments of the present invention, extraction is performed using ethanol:ethyl acetate in a ratio of 4:1. The pest control effect of this ginger extract is significantly superior to that of ethanol extract or ethyl acetate extract used alone.

[0019] In this invention, the statement that "extracts can be obtained by extracting from two or more ginger plants" refers to extracting two or more ginger plants simultaneously in the same container and solvent. For example, ginger and turmeric can be placed in the same container and extracted at the same time.

[0020] The extraction process further includes other standard steps such as filtration, concentration, centrifugation, drying, and evaporation. For convenience of storage, transport, and use, solvent extracts other than volatile oils may be dried after extraction by removing the solvent. Methods for solvent removal include, but are not limited to, atmospheric evaporation, reduced-pressure evaporation, thin-film evaporation, and spontaneous evaporation.

[0021] To extract as many biologically active components as possible from ginger plants, the raw materials from ginger plants can be pulverized.

[0022] In some embodiments of the present invention, the ratio of the solvent added during extraction to the ginger plant raw material ("liquid ratio") is generally selected from 4 to 60:1 (mL / g, L / Kg, etc.), for example, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1. , 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1, 37:1, 38:1, 39:1, 40:1, 42:1, 43:1, 44:1, 45:1, 46:1, 47:1, 48:1, 49:1, 50:1, 55:1, 60:1, etc. are also acceptable.

[0023] The "liquid-to-raw ratio" is the ratio of the ginger plant raw material to the solvent, and it is the reciprocal of the "liquid-to-raw ratio."

[0024] In some embodiments of the present invention, during heat extraction, it can be extracted by heat leaching or heat reflux, and the temperature during extraction can be, but is not limited to, 20°C to 95°C. For example, it can be 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C, 95°C, etc.

[0025] The number of extractions can be selected as needed, and generally it is 1 to 4 times, and the number of extractions is more often 2 or 3 times.

[0026] In addition, the extraction conditions of the normal extraction methods such as ultrasonic extraction, microwave extraction, and supercritical extraction of the present invention can be appropriately adjusted by those skilled in the art.

[0027] In some embodiments of the present invention, in the extraction process of Zingiber rhizome, the material-liquid ratio is 1:20 to 60, the extraction time is 1 to 4 h, and the number of extractions is 1 to 3 times.

[0028] In the present invention, for volatile oils, ordinary extraction methods are used, mainly including steam distillation method, leaching method, adsorption method, etc. ("Process Chemistry of Plant Natural Products Extraction", Chemical Industry Press, July 2022, pages 100 - 110).

[0029] In some embodiments of the present invention, the volatile oil of Curcuma zedoaria rhizome is prepared by the steam distillation method. The material-liquid ratio is 1:15 to 25, the distillation time is 5 h or more, and the sodium chloride concentration in water is 2 to 4%. The concentration of sodium chloride is generally the mass-volume percentage. For example, 2 to 4 g of sodium chloride is contained in 100 mL of water.

[0030] The Zingiberaceae plant extract may be the only active ingredient, or it may be used in combination with other insecticides, acaricides, and fungicides, including but not limited to natural plant extracts, synthetic substances, etc.

[0031] Based on the synergistic effect of the insecticidal activity of the combination of ginger and turmeric, the present invention further provides an insecticidal composition. It includes a mixture of ginger rhizome extract and turmeric rhizome extract in a mixing ratio of 1 to 9:9 to 1. Here, the ginger rhizome extract is extracted from ginger rhizome with ethanol:ethyl acetate = 1 to 4:1, and the turmeric rhizome extract is volatile oil of turmeric rhizome.

[0032] The ratio of ethanol to ethyl acetate may be 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, etc.

[0033] Furthermore, the ratio of ginger rhizome extract to turmeric rhizome extract is 7-9:3:-1.

[0034] Preferably, the mixture consists of ginger rhizome extract and turmeric rhizome extract in a ratio of 7:3. Studies have shown that this ratio significantly enhances the pest control effects of both ginger and turmeric extracts, and that they exhibit a synergistic effect at this specific ratio.

[0035] In this invention, the mixing ratio between extracts is generally expressed as a mass ratio.

[0036] In the present invention, solvent extracts other than volatile oils may be dried products after the solvent has been removed, or they may be products containing a specific amount of solvent.

[0037] When mixing ginger rhizome extract and turmeric rhizome extract in proportion, the amounts of other extracts besides volatile oil are calculated based on the dried product. However, this does not mean that all solvent extracts in this invention are only dried products from which the solvent has been removed. In this invention, extracts containing solvents can also be used in the mixing process, and the proportions can be calculated by determining the amount of dried product in the solvent-containing extract based on its concentration. For example, in a 4:1 ethanol:ethyl acetate extract of ginger rhizome without solvent removal, if it has not been completely concentrated and dried, the solvent content is 80%. If you want to mix ginger rhizome extract (dried product after solvent removal) and turmeric rhizome volatile oil in a 7:3 ratio, you need to mix the ginger rhizome extract (without solvent removal) and turmeric rhizome volatile oil in a 35:3 ratio.

[0038] In the extraction process of ginger rhizome, the liquid-to-liquid ratio is 1:20 to 60, the extraction time is 1 to 4 hours, and the number of extractions is 1 to 3. Turmeric rhizome volatile oil is prepared by steam distillation, with a liquid-to-liquid ratio of 1:15 to 25, a distillation time of 5 hours or more, and a sodium chloride concentration in water of 2 to 4%.

[0039] In some embodiments of the present invention, the total amount of ginger rhizome extract and turmeric rhizome extract in the composition is 0.1 to 100%, including any content in between, for example, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 5%, 7.5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, or 99%.

[0040] In addition to the mixture, the composition may further contain one or more of α-curcumene, 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone, and 1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl)sulfite. Studies have shown that combining these compounds with the mixture can also improve the pest control effect and produce a synergistic effect.

[0041] For example, a mixture of α-curcumene = 1:2~8; a mixture of 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone = 1:2~8; and a mixture of 1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl)sulfite = 1:2~8.

[0042] In the present invention, the ginger plant Zingiber officinale Rosc. is derived from the ginger plant Kaempferia galanga L.

[0043] The extraction method of the present invention can be referenced from the following prior art. (1) Zhang Dongmei, Yuan Ting. Study on the process of extracting volatile oil from ginger by steam distillation [J]. Agricultural Technology and Equipment, 2023, (02):27-28+32. (2) Liu Jiangwei; Ye Fei. A study on the process of extracting ginger essential oil by steam distillation [J]. Modern Agricultural Research, 2018(04) (3) Sun Jiayi. Research progress on the extraction and development and utilization of ginger essential oil [J]. Shandong Chemical, 2019(24) (4) Yang Shiwei. Study on the extraction of ginger oil and microcapsule embedding process using supercritical CO2 [D]. Tianjin University of Science and Technology, 2015. (5) Chen Qi. A study on the extraction of turmeric volatile oil by microwave and steam distillation. [J]. Chemical Engineering Management, 2015, (12):2-3. (6) Liu Xuemei, Liang Jianqin, Lu Bin, et al. Chemical composition study of volatile oils of fresh and dried turmeric extracted by supercritical carbon dioxide [J]. Shizhen Guoyi Guoyue, 2008, (08):2007-2009. (7) Ling Yu-Zhao. A study on the extraction of characteristic components and oil from turmeric by steam distillation. [J]. Chinese Seasonings, 2005, (08):28-31. (8) Chen Qingsheng, He Jingyu, Meng Xiao, et al. A study on the hair loss prevention and dandruff prevention effects of three types of ginger plant active extracts [J]. Guangdong Chemical, 2021, 48(22):70-72. (9) Wu Cunbing, Shao Bojin, Wu Junyan, et al. Optimization and stability of the ginger flavonoid ethanol leaching process [J]. Southern Agricultural Research Journal, 2020, 51(11):2798-2807. (10) Rao Jia-rui, Zhang Xin, Yu Yong-xiu. Extraction of natural products of ginger and indoor toxicity strength against tea leaf blight [J]. Agricultural Technology Service, 2021, 38(08):31-34. (11) Zhou Yeyan. Research on microwave extraction processes and applied technologies for spices [D]. Guangzhou University, 2011. (12) Wu Xiaofei. Study on the inhibitory effect of turmeric extract on dominant spoilage bacteria in poultry products [D]. Qingdao University of Science and Technology, 2020. (13) Hirayuki Rei. Extraction and analysis of active ingredients in Yamana and their application in composite membranes [D]. Chongqing University of Technology and Commerce, 2022.

[0044] In the use described in the present invention, the ginger plant extract can be used as is as a single agent.

[0045] The insecticidal composition described in the present invention can also be used as a single agent.

[0046] The term "single agent" as used in this invention refers to a product in which only a ginger plant extract is used as the sole ingredient, without the addition of any other auxiliary agents.

[0047] For product stability and preservation during transport, ginger plant extracts can be prepared in the corresponding dosage form along with additives. The additives may be common additives in the art, such as surfactants and solvents.

[0048] A suitable surfactant can be selected by those skilled in the art as required by the actual circumstances. Examples of surfactants usable in embodiments of the present invention include, but are not limited to, ethoxylated castor oil, sodium lauryl sulfate, saponins, ethoxylated alcohols, ethoxylated fatty acid esters, alkoxylated glycols, ethoxylated fatty acids, carboxylated alcohols, carboxylic acids, fatty acids, ethoxylated alkylphenols, fatty acid esters, sodium lauryl sulfide, other fatty acid-based surfactants, other natural or synthetic surfactants, and combinations thereof. In some embodiments, the surfactant is a nonionic surfactant. In some embodiments, the surfactant is an ionic surfactant. The selection of a suitable surfactant depends on the relevant application and conditions of use, and suitable surfactants are known to those skilled in the art.

[0049] In the present invention, the dosage form includes, but is not limited to, emulsions, soluble powders, soluble granules, solutions, dispersible liquids, aqueous emulsions, microemulsions, microcapsule suspensions, seed treatment liquids, aerosols, and the like.

[0050] Emulsions are a type of pesticide formulation, made by dissolving a relatively high concentration of active ingredients in a solvent and adding an emulsifier. Generally, they are diluted with a large amount of water to form a stable emulsion, which is then sprayed using a sprayer. Low-volume or very low-volume spraying is also possible. They can be used as is or diluted with water before spraying.

[0051] Wettable powders are very fine drying agents obtained by mixing and grinding active pharmaceutical ingredients, excipients, surfactants, and other additives.

[0052] A suspension is a formulation in which a solid active pharmaceutical ingredient is uniformly dispersed in water as particles smaller than 4 μm. Its international code is SC, and it typically has a particle size of 0.1-3 μm and a high suspension efficiency. Suspensions are divided into two types: aqueous suspensions and oily suspensions. Aqueous suspensions use water as the suspension medium, while oily suspensions use oil as the suspension medium and do not contain water. Commonly used oils include vegetable oils such as corn oil and rapeseed oil. Suspensions completely eliminate the need for organic solvents and are a suitable dosage form for processing solid raw materials. A suspension is a mixture of a solid powder and a liquid suspended in water, and it needs to be shaken and diluted with water before use for spraying. Suspensions are easy to carry and dilute, can be sprayed uniformly, and have excellent adhesion and durability.

[0053] Powdered formulations refer to the powdered form of the active ingredient, or a powder prepared by adding a specific diluent. They can be applied directly with a simple duster, resulting in high work efficiency, low adhesion and residue to crops, and minimal phytotoxicity.

[0054] Granules, or granular formulations, are solid forms produced by mixing an active pharmaceutical ingredient with additives such as carriers, adhesives, dispersants, wetting agents, and stabilizers. Their performance requirements primarily include fineness, uniformity, storage stability, hardness, and disintegration properties. Granules have the largest particle size among solid forms, ranging from 300 to 1700 μm, making them easy to use, with minimal outward diffusion and long-lasting effects.

[0055] Aqueous formulations are solutions of the active pharmaceutical ingredient (API). The drug is uniformly dispersed in water in an ionic or molecular state. The concentration of the drug depends on the water solubility of the API, and is generally the maximum solubility; water is added to dilute it before use.

[0056] An odor masking agent may be added during use. In some embodiments, the odor masking agent is vanilla extract, wintergreen oil, spearmint oil, clove oil, lemongrass oil, and / or a combination thereof.

[0057] An odor neutralizer may be added during use. In some embodiments, the odor neutralizer may be an odor-absorbing material, such as zeolite and / or other natural or synthetic odor-absorbing material.

[0058] The mother liquor or mother powder is a semi-finished product processed from the active pharmaceutical ingredient and requires further processing to become the final product.

[0059] In this invention, a liquid dosage form is prepared using an extract from a plant of the Zingiberaceae family. When controlling mites or thrips, the concentration of the Zingiberaceae plant extract in the liquid dosage form can be selected according to the actual requirements.

[0060] In some embodiments of the present invention, the composition further comprises a solvent. The total concentration of the ginger rhizome extract and the turmeric rhizome extract is 0.1 mg / mL or more. The total concentration refers to the concentration in the composition of the mixture of ginger rhizome extract and turmeric rhizome extract.

[0061] Furthermore, the total concentration of ginger rhizome extract and turmeric rhizome extract may be 0.1 to 1000 mg / mL, and may also be 0.1 to 500 mg / mL, 0.1 to 200 mg / mL, 0.1 to 100 mg / mL, 0.1 to 50 mg / mL, 1 to 500 mg / mL, 1 to 200 mg / mL, 1 to 100 mg / mL, 1 to 50 mg / mL, 1 to 10 mg / mL, 1 to 5 mg / mL, 2 to 200 mg / mL, 2 to 100 mg / mL, 2 to 50 mg / mL, 2 to 10 mg / mL, 3 to 200 mg / mL, 3 to 100 mg / mL, 3 to 50 mg / mL, 3 to 10 mg / mL, 4 to 200 mg / mL, 4 to 100 mg / mL, 4 to 50 mg / mL, or 4 to 10 mg / mL. Specifically, 0.1mg / mL, 0.2mg / mL, 0.3mg / mL, 0.4mg / mL, 0.5mg / mL, 0.6mg / mL, 0.7mg / mL, 0.8mg / mL, 0.9mg / mL, 1mg / mL, 1.1mg / mL, 1.2mg / m L, 1.3mg / mL, 1.4mg / mL, 1.5mg / mL, 1.6mg / mL, 1.7mg / mL, 1.8mg / mL, 1.9mg / mL, 2.0mg / mL, 2.5mg / mL, 3mg / mL, 3.5mg / mL, 4mg / mL, 4.5mg You may choose from 1 / mL, 5mg / mL, 5.5mg / mL, 6mg / mL, 6.5mg / mL, 7mg / mL, 7.5mg / mL, 8mg / mL, 8.5mg / mL, 9mg / mL, 9.5mg / mL, 10mg / mL, 11mg / mL, 12mg / mL, 13mg / mL, 14mg / mL, 15mg / mL, 20mg / mL, 25mg / mL, 30mg / mL, 35mg / mL, 40mg / mL, 45mg / mL, 50mg / mL, 100mg / mL, etc., but these are not available.

[0062] In some embodiments, the solvent may be water, ketones, alcohols, aldehydes, ethers, esters, carboxylic acids, and may include non-aryl ketones, non-aryl alcohols, non-aryl aldehydes, non-aryl esters, non-aryl carboxylic acids, aryl alcohols, aryl alkyl alcohols, aryl aldehydes, aryl alkyl ketones, arylaryl ketones, aryl carboxylic acids, aryl alkyl esters, aryl aryl esters, aryl alkyl ethers, aryl aryl ethers, and / or combinations thereof.

[0063] In some embodiments, the solvent includes ethanol, isopropyl alcohol, benzyl alcohol, acetone, acetophenone, water, citric acid, lactic acid, glycerin, castor oil, benzoic acid, carbonic acid, ethoxylated alcohol, ethoxylated amide, glycerides, butanol, 1-propanol, hexanol, other alcohols, dimethyl ether, polyethylene glycol, and the like.

[0064] The present invention further provides a method for killing and / or controlling pests, and / or eradicating pests, and / or preventing or reducing pest oviposition, and / or preventing or reducing the hatching of pest eggs, the method comprising exposing pests and / or pest eggs to the above-mentioned insecticidal composition.

[0065] In the above method, the concentration of the insecticidal composition added may be 0.1 to 1000 mg / mL of a mixture (dried product) of ginger rhizome extract and turmeric rhizome extract, or it may be 0.1 to 500 mg / mL, 0.1 to 200 mg / mL, 0.1 to 100 mg / mL, 0.1 to 50 mg / mL, 1 to 500 mg / mL, 1 to 200 mg / mL, 1 to 100 mg / mL, 1 to 50 mg / mL, 1 to 10 mg / mL, 1 to 5 mg / mL, 2 to 200 mg / mL, 2 to 100 mg / mL, 2 to 50 mg / mL, 2 to 10 mg / mL, 3 to 200 mg / mL, 3 to 100 mg / mL, 3 to 50 mg / mL, 3 to 10 mg / mL, 4 to 200 mg / mL, 4 to 100 mg / mL, 4 to 50 mg / mL, or 4 to 10 mg / mL. Specifically, 0.1mg / mL, 0.2mg / mL, 0.3mg / mL, 0.4mg / mL, 0.5mg / mL, 0.6mg / mL, 0.7mg / mL, 0.8mg / mL, 0.9mg / mL, 1mg / mL, 1.1mg / mL, 1.2mg / m L, 1.3mg / mL, 1.4mg / mL, 1.5mg / mL, 1.6mg / mL, 1.7mg / mL, 1.8mg / mL, 1.9mg / mL, 2.0mg / mL, 2.5mg / mL, 3mg / mL, 3.5mg / mL, 4mg / mL, 4.5mg You may choose from 1 / mL, 5mg / mL, 5.5mg / mL, 6mg / mL, 6.5mg / mL, 7mg / mL, 7.5mg / mL, 8mg / mL, 8.5mg / mL, 9mg / mL, 9.5mg / mL, 10mg / mL, 11mg / mL, 12mg / mL, 13mg / mL, 14mg / mL, 15mg / mL, 20mg / mL, 25mg / mL, 30mg / mL, 35mg / mL, 40mg / mL, 45mg / mL, 50mg / mL, 100mg / mL, etc., but these are not available.

[0066] The insecticidal composition may include a mixture of ginger rhizome extract and turmeric rhizome extract, as well as other products. These may include, but are not limited to, one or more other insecticides, acaricides, fungicides, herbicides, plant growth regulators, fertilizers, and compounds that perform equivalent functions but have not yet been commercialized. This may result in further advantages and effects. For example, other insecticides may include fluopifranone, deltamethrin, ethinyl, tetrazopyramide, imidacloprid, spirotetramato, spirodiclofen, diprocyclap, fenfenthoril, cis-cypermethrin, bromifenamide, etofenyl, beta-cyhalothrin, pymetrozine, thiamethoxam, lufenuron, abamectin, chlorantraniliprole, bifenthrin, cyantraniliprole, fenflufenthrin, spinosad, triflufenacil, sulfoxaflor, pirimiphosmethyl, indoxacarb, dinotefuran, dinotefuran, dimethonate, flufenhydrazone, permethrin, flufurfenuron, and flufendifen.

[0067] The concentrations of other products can be adjusted as needed. For example, if it is necessary to add α-curcumene, 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone, or 1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl) sulfite, the amounts of the other products can be calculated using a mixture of ginger rhizome extract and turmeric rhizome extract, with the following ratios: mixture:α-curcumene = 1:2~8; mixture:5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone = 1:2~8; mixture:1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl) sulfite = 1:2~8.

[0068] As used in this invention, "contains" or "includes" shall be interpreted in their open sense. That is, the presence of any particular feature, element, step, or component mentioned shall not preclude the presence or addition of any further features, elements, steps, or components.

[0069] As used in this invention, the term "pest" refers to an organism that adversely affects a host, such as a plant or an animal like a mammal, by parasitizing, damaging, attacking, competing for nutrients, or infecting the host.

[0070] Pests include, but are not limited to, arthropods (including insects and spiders), blood-sucking pests, and biting pests (bed bugs, mites, ticks, ants, lice, cockroaches, thrips, etc.).

[0071] In some embodiments of the present invention, the pest is one or more species selected from the following: Thyrmeptoptera, Hemiptera, Lepidoptera, Spider mites, Lesser spider mites, Gall mites, Dust mites, Lice mites, Claw mites, Cheyletiidae, Sarcoptes scabiei, Dust mites, and Dust mites.

[0072] The aforementioned "Thymbryata" (thrips) belongs to the class Insecta, and insects of this order are called "thrips." Thrips are divided into the suborders Blastora and Tuberculata. The suborder Blastora includes the superfamilies Aeolothripoidea (Aeolothripidae, Orothripidae, Melanthripidae, Dactuliothripidae, Franklinothripidae), Merothripoidea (Aeolothripoidea), and Thripoidea (Heterothripidae, Hemithripidae, Ceratothripidae, Panchaetothripidae, and Thripidae). Here, the family Thripidae is the most important family in this order. It is known that this family contains 33 genera and approximately 200 species. For example, species such as the flat-headed thrips, onion thrips, bean thrips, rice thrips, flower thrips, southern yellow thrips, orange yellow thrips, loquat thrips, sugarcane thrips, cajonka thrips, tea yellow thrips, croton thrips, tea yellow thrips, and Scorotripsy sexmaculatus are commonly found in China. The suborder Tubula includes the superfamilies Phlaeothripoidea (Pypothripidae, Ecacanthothripidae, Eupatithripidae, Phlaeothripidae, Chirothripoididae, Hystricothripidae, Idolothripidae, Megathripidae) and Urothripoidea (Urothripidae). The families listed in parentheses after the superfamilies are all subfamilies within those superfamilies, and this method will be used in the following explanation.

[0073] The aforementioned "Hemiptera" belongs to the class Insecta. Hemiptera is divided into the suborders Dorcirrhira and Stomachrophira. Dorcirrhira includes the superfamily Cicadidae (Cicadas, Treehoppers, Macearotidae, Spittlebugs, and Auchenorrhynchae) and the superfamily Eurybrachydidae (Planthopods, Planthoppers, Dracaena, Eurybrachydidae, Scutellaria, Scutellaria, Scutellaria, Lacecapsidae, Scutellaria, Scutellaria, Scutellaria, and Dracaena). The suborder Stomachromorpha includes the superfamilies Psyllidae (Psyllidae), Dipteroideae (Dipteroideae), Aphidoidea (Phylloxera, Phylloxera, Aphididae), and Coccoidea (Coccidae, Kerridae, Coccidae, Coccidae, Coccidae, Coccidae, Coccidae).

[0074] The aforementioned "Lepidoptera" belongs to the class Insecta, is widely distributed, with the largest number of species found in tropical regions, and the larvae of most species cause damage to various cultivated plants. Larger species feed on dried leaf fragments or branches. Lepidoptera includes the suborders Encarpalidae (Encarpalidae), Monophyla (Eriocraniidea, Heliocranioidea, Stigmelloidea, and Bombyxidae), and Diphyla (Tineoidea, Psychoidea, Psychoidea, Castnioidea, Tortricidae, Pyralidae, Bombyx, Anchoridae, Geometridae, Sphingidae, Noctuoidae, Hesperioidae, and Papilionidae).

[0075] The term "mites" as used in this invention mainly includes agricultural pest mites, and most belong to the families Tetranychidae, Tenuipalpidae, Eriophyidae, Tarsonemidae, Pyemotidae, Pentaleidae, and Cheytidae within the class Acachnida.

[0076] The family Miridae includes genera such as Oligonyx (e.g., Oligonyx baipisongis, Larch spider mite, Camellia spider mite, etc.), Eotetranyx (e.g., Eotetranyx albus, Eotetranyx bailae, Camellia spider mite, etc.), Spider mite (e.g., Southern spider mite, Sagami spider mite, Two-spotted spider mite, False two-spotted spider mite), Formosanus (e.g., Ryujin spider mite, Bamboo spider mite, Schizotetranyx elongatus, etc.), and Mixonychus (e.g., Mixonychus (Bakerina) aesteva, Mixonychus ganduis, Mixonychus (Bakerina)) (e.g., murrayae), Panonychus (e.g., citrus spider mite, Panonychus cagleyi, apple spider mite), Allonychus (e.g., Allonychus bambusae, Allonychus winicus), Stigmaeopsis (e.g., bamboo spider mite, Stigmaeopsis nanjingensis), Mononychellus (e.g., Mononychellus georgicus), Acanthonychus (e.g., Acanthonychus jampengensis), Amphitetranychus (e.g., cherry spider mite), Sonotetranychus (e.g., Sonotetranychus neosalix), Xinella (e.g., Xinella huangshanensis), Yunoychus (e.g., Yunoychus daliensis), Neotetranychus (e.g., Neotetranychus lek), Eurytetranychus (e.g., Eurytetranychus glycyrrhizae, Eurytetranychus wuyishanensis), Aponychus genus (e.g., Aponychus aequilibris, Aponychus corpuzae), Eutetranychus genus (e.g., Eutetranychus orientalis, Eutetranychus xianensis), Stylophoronychus genus (e.g., StylophoronychusThey are divided into genera such as baghensis, Eurytetranychoides (e.g., *Eurytetranychoides*), Tenuipalpoides (e.g., *Tenuipalpoides hastata*, *Tenuipalpoides zizyphus*), Bryobia (e.g., *Bryobia borealis*, *Bryobia exserta*), Sinobryobia (e.g., *Sinobryobia chinensis*), Petrobia (e.g., *Petrobia (Petrobia) xinjiangensis*, *Petrobia (Tetranychina) zachvatkini*, etc.), *Tetranycopsis hystriciformis*, *Tetranycopsis spiraeae*, etc.), Apronobia (e.g., *Apronobia alkalisalinae*), Mesobriobia (e.g., *Mesobriobia terpogossiani*), and Doliconobia (Doliconobia altaiensis).

[0077] In some embodiments of the present invention, the pests are the flat-headed thrips, onion thrips, bean thrips, rice thrips, flower thrips, southern yellow thrips, citrus yellow thrips, loquat thrips, sugarcane thrips, cajonka thrips, tea yellow thrips, and croton thistle. Horse, tea yellow thrips, Scorotryps sexmaculatus, leaf borer, white-spotted armyworm, beet armyworm, peach fruit moth, tobacco budworm, diamondback moth, cotton-covered black leaf borer, bean borer, tobacco whitefly, greenhouse whitefly, citrus whitefly, citrus whitefly, bayberry whitefly, A The selection is made from Leurosibotus indicus, pyraling whitefly, Oligonyx baipisongis, larch spider mite, sedge spider mite, Eotetranicus albus, Eotetranicus bailae, camellia spider mite, nansei spider mite, sagami spider mite, two-spotted spider mite, false two-spotted spider mite, Ryujin spider mite, bamboo spider mite, Schizotetranicus elongatas, Mixonyx (Bacherina) aesteva, Mixonyx ganjuis, citrus spider mite, Pannonius cagley, Arronix bambusae, Arronix winicus, bamboo burrowing spider mite, Mononicerus georgicus, Acanthonius jampengensis, and cherry spider mite.

[0078] In some embodiments of the present invention, the pests may be selected from pear psyllids, leafhoppers, lily-tailed beetles, lacewings, fire ants, termites, codling moths, mosquitoes, flies, cockroaches, fleas, and the like.

[0079] In this invention, "insecticide" refers to the killing and / or control of pests.

[0080] The term "control" as used in this invention refers to, but is not limited to, arbitrary killing, growth regulation, and pest control (inhibition or interference with the normal life cycle of pests) activities against pests (including, for example, contraceptive activities that prevent the production of eggs or sperm, kill sperm or eggs, or cause significant damage to genetic material). It is intended that other activities within the scope of the term "control" are also included. These include preventing larvae from developing into mature offspring, regulating the emergence of pests from eggs (including prevention of hatching), breaking down egg material, suffocating, reducing intestinal motility, inhibiting chitin formation, interfering with mating or sexual communication, and preventing feeding (antifeeding) activity.

[0081] In the present invention, "exterminator" or "extermination" means preventing pests from falling or climbing onto a surface to which a ginger plant extract or insecticide composition has been applied or introduced, and / or promoting the removal of pests from a surface to which a ginger plant extract or insecticide composition has been applied or introduced.

[0082] The “surface” or “target surface” described in the present invention includes surfaces to which an insecticide is applied or is planned to be applied. Such surfaces may include, for example, surfaces to which pests may come into contact with or be exposed to the applied insecticide and lay eggs, and / or surfaces that are infested with or suspected to be infested with pests.

[0083] In this invention, "prevention of egg-laying" refers to preventing pests from laying eggs and reducing the number of eggs that pests normally lay.

[0084] In this invention, "prevention of hatching" refers to preventing or delaying the hatching of larvae from eggs.

[0085] The term "insecticide" as used in this invention refers to a reagent capable of controlling and / or killing pests.

[0086] In some embodiments, the methods of using the above-mentioned optional compositions in the present invention include direct application, dilution with a suitable vehicle and application as a spray, or application as a diluted concentrate.

[0087] In the present invention, in order to obtain the desired effect, in some embodiments, the dose of the extract or the composition of the extract (insecticide composition) is varied depending on various factors such as the crop to be protected, the type of pest, the degree of infection, the method of pesticide application, the environment in which the pesticide is applied, and the dosage form.

[0088] In the present invention, the effective amount is not particularly limited. For example, when used in a field, an extract or extract composition (insecticide composition) at a dose of 10 g to 1 kg per hectare can be sprayed to ensure sufficient control.

[0089] In the present invention, the killing and / or control of pests, the extermination of pests and / or pests, and / or the prevention or reduction of pest oviposition and / or the prevention or reduction of pest eggs are not limited to use in the agricultural field, and should be included in the scope of protection of the present invention whenever the killing and / or control of pests, the extermination of pests and / or pests, and / or the prevention or reduction of pest oviposition and / or the prevention or reduction of pest eggs are required.

[0090] The beneficial effects of the present invention are, but are not limited to, those listed below. (1) We were the first to discover that extracts from ginger plants (ginger and turmeric) have excellent anti-insect activity against agricultural pests such as mites and thrips. (2) Optimize the extraction process to improve the insecticidal, ovicidal, and pesticide activity of ginger plant extracts. (3) We discovered a synergistic effect of extracts from different Zingiberaceae plants. Specifically, a mixed solvent extract of ginger rhizome in ethanol and ethyl acetate showed a significantly improved pest control effect compared to a single solvent extract. Furthermore, a mixture of ethanol and ethyl acetate extracts of ginger rhizome with turmeric volatile oil significantly improved pest control effects and exhibited a synergistic effect. [Modes for carrying out the invention]

[0091] The following describes the technical solutions of this application clearly and completely, however, the examples described are, of course, only some, of the examples of this application, not all of them. Based on the examples in this application, all other examples that can be obtained without creative work by a person skilled in the art are all within the scope of protection of this application. Unless otherwise specified, the following can be realized or understood by a person skilled in the art by reference to the prior art. The reagents or equipment used are not specified by the manufacturer and can be considered to be ordinary products obtained by commercial purchase.

[0092] When a numerical range is provided, it is understood that each intermediate value between the upper and lower limits of that range (one-tenth to the unit of the lower limit, unless the context otherwise explicitly indicates), and other predetermined or intermediate values ​​within that given range are included in embodiments of the Application. The upper and lower limits of these smaller ranges can independently define smaller numerical ranges, and these smaller ranges are intended to cover limits that are arbitrarily and explicitly excluded within the given range in embodiments of the Application.

[0093] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Any methods and materials similar or analogous to those described herein may be used in the practical or experimental applications of the embodiments of this application, but preferred methods and materials are described to avoid unnecessarily complicating the disclosure.

[0094] In a specific embodiment of the present invention, the ginger used is obtained from thinning in a field, and the turmeric used is purchased as a commercially available product.

[0095] The ginger extract, ginger volatile oil, turmeric extract, and turmeric volatile oil of the present invention may be obtained by purchasing commercially available products or prepared by conventional extraction methods. The extraction methods in the following examples of this application merely refer to common sense to prepare the raw materials used in the experiment and should not be understood as limiting the method of obtaining the raw materials.

[0096] In specific embodiments of the present invention, the raw materials for preparation methods not otherwise specified may be obtained by purchasing commercially available products or may have already been prepared by method.

[0097] Example 1 (Method for preparing experimental extracts) Appropriate amounts of coarse powder from the whole plant and rhizome of ginger were taken, and each was ultrasonically extracted for 1 hour using one of the following solvents: water, ethanol (anhydrous ethanol), ethyl acetate, or methanol, in a solvent-to-liquid ratio of 1:40. The extraction was repeated twice, the two extracts were combined, and the mixture was concentrated under reduced pressure until dry to obtain ginger whole plant extract (water extract, ethanol extract, ethyl acetate extract, methanol extract) and ginger rhizome extract (water extract, ethanol extract, ethyl acetate extract, methanol extract), respectively.

[0098] Appropriate amounts of coarse powder from the whole plant and rhizome of turmeric were taken, and each was ultrasonically extracted for 1 hour in a solvent of water, ethanol (aqueous ethanol), ethyl acetate, or methanol at a solvent ratio of 1:40. The extraction was repeated twice, the two extracts were combined, and the mixture was concentrated under reduced pressure until dry to obtain whole plant extract of turmeric (water extract, ethanol extract, ethyl acetate extract, methanol) and rhizome extract of turmeric (water extract, ethanol extract, ethyl acetate extract, methanol), respectively.

[0099] Appropriate amounts of coarse powder from the whole plant, roots, and tubers of ginger were taken and placed in a steam distillation apparatus, with a liquid-to-water ratio of 1:10. A heating device was started, and after the volatile oil stopped flowing out, the heating was stopped and the mixture was allowed to cool naturally to room temperature. The volatile oil was collected from a volatile oil extraction device to obtain volatile oil from the whole plant of ginger and volatile oil from the rhizome of ginger, respectively.

[0100] Appropriate amounts of coarse powder from the whole turmeric plant, roots, and tubers were taken and placed in a steam distillation apparatus. The liquid-to-water ratio was set to 1:10, and the heating device was started. After the volatile oil stopped flowing out, the heating was stopped and the mixture was allowed to cool naturally to room temperature. The volatile oil was collected from the volatile oil extraction apparatus to obtain volatile oil from the whole turmeric plant and volatile oil from the turmeric rhizome, respectively.

[0101] The term "whole plant" refers to the above-ground parts of a plant.

[0102] (Indoor activity measurement of adult mites (using *Tetranychus malaria* as an example)) A 4cm piece of double-sided tape was attached to one end of a microscope slide. Using a small brush, a false spider mite was gently lifted, and the tape was lightly applied to its back, ensuring that it did not adhere to the mite's legs, antennae, or mouthparts. At least 30 mites were attached to each slide, arranged in a line, and placed in a clean culture dish. Filter paper was placed in the dish to maintain moisture in the filter paper, and the lid was closed. After 2 hours, each mite was observed under a stereomicroscope. If death was found, the dead bodies were removed, and if the number fell below 30, replenishment was necessary. The test agent (when using the agent of the present invention, it is prepared with an appropriate amount (e.g., 10%) of anhydrous ethanol. It is supplemented with a surfactant (e.g., 5% castor oil polyoxyethylene ether) and sterile water. For the solvent control, the components of the test agent, such as the solvent and surfactant, were not changed, and the test sample was changed to the same weight of sterile water only) was uniformly dispersed in water. After preparing the solution at the test design concentration (Table 1), one end of a glass slide to which healthy false spider mites were attached was immersed in the prepared solution, the slide was gently shaken, and after 5 seconds it was removed. Excess solution was absorbed with absorbent paper, and then the slide was placed in the culture dish described above. After the solution was dried, the lid was closed and the mixture was cultured at 25°C (experimental errors can be reduced by leaving the insects attached for a while before examining them under a microscope. For example, some insects may die spontaneously and non-pharmacologically after being attached). After 72 hours, the number of dead false spider mites was recorded using a stereomicroscope. False spider mites were considered dead if they did not react when lightly touched with a small brush. The control effect was calculated using the following formula.

[0103] Population density reduction rate (%) = (Population density before treatment - Number of worms after treatment) / Population density before treatment × 100 Control effectiveness (%) = (Percentage reduction in population density of the treated group - Percentage reduction in population density of the control group) / (100 - Percentage reduction in population density of the control group) × 100

[0104] (Indoor activity measurement of mite eggs (using *Tetranychus spp.* as an example)) Twenty adult female spider mites were transferred to a 2.0 cm diameter broad bean leaf (with a wet filter paper at the bottom), covered with a lidded culture dish, and then cultured in a humid environment. Adult mites were removed within 36 hours, and the number of eggs on the leaves was counted under a microscope. The number of eggs on each leaf was examined and recorded under a microscope before immersion in the pesticide solution. Leaves containing mite eggs were immersed in clean water for 10 seconds, removed, and then cultured in a humid environment. Each treatment was repeated at least three times. The mite eggs and leaves after treatment were cultured under normal conditions. Five days after application, the hatching status of the spider mite eggs was examined. The control effect was calculated using the following formula.

[0105] Egg hatching rate (%) = Number of hatched eggs / Total number of processed eggs * 100 Control effectiveness (%) = (Egg hatching rate in control group - Egg hatching rate in treated group) / Egg hatching rate in control group) * 100

[0106] (Eradication experiment (using thrips as an example)) When conducting behavioral tests on adult thrips using a "Y" type olfactory meter, a bottle containing the test substance's odor source was connected to one side arm of the "Y" type olfactory meter, and bottles containing liquid paraffin's odor source were connected to the other two sides as controls. Air was passed through for 5 minutes to fill the tubing with the odor. The airflow rate of the 4-arm olfactory meter was controlled to 200 mL / min, the room temperature was controlled to (25 ± 1) °C, and the relative humidity was controlled to 60% to 80%. The base arm end of the glass tube of the "Y" type olfactory meter was introduced to adult thrips that had been starved for 5 hours, and a single 10-minute test was conducted. If an adult thrips crawled beyond 1 / 3 of the side arm connected to the odor source bottle and remained there for more than 30 seconds, it was recorded that the adult had selected this arm, a positive response occurred, and it was recorded as induction. On the other hand, if the adult crawled to a different side arm, a negative response occurred, and it was recorded as extermination. If no selection was made within 5 minutes, it was recorded as no reaction. Five test thrips were measured for each treatment, and the process was repeated three times. For the control, liquid paraffin was used as the odor source. Each thrips was tested only once. The eradication rate was calculated using the following formula.

[0107] Eradication rate (%) = Number of thrips eradicated / Total number of thrips * 100

[0108] (Thrips egg test) Ten thrips were transferred to a 2.0 cm diameter kidney bean leaf (with a wet filter paper at the bottom), covered with a lidded culture dish, and then cultured in a humid environment. Adults were removed 24 hours after egg-laying. Leaves containing thrips eggs were immersed in clean water for 30 seconds, removed, and then cultured in a humid environment. Each treatment was repeated at least three times. Treated leaves were cultured under normal conditions. Seven days after application, the hatching status of thrips larvae was investigated. The control effect was calculated using the following formula.

[0109] Control effectiveness (%) = ((Number of larvae in control group - Number of larvae in treated group) / Number of larvae in control group) * 100

[0110] (Thrips adult test) Hydroponically grown green bean seedlings with only one true leaf were left for 24 hours next to plants infested with thrips, waiting for adult thrips to crawl onto the leaves, or they were transferred to green bean seedlings where healthy thrips had been treated with the tip of a wet calligraphy brush. There were more than 50 thrips in each plant.

[0111] Before application, the base number of populations on each treated plant was investigated, and relatively small and dead insects were removed to ensure that the size and number of insects on each treated plant were basically the same and at least 50. The test solution was sprayed onto the corresponding test plant using a watering can, ensuring uniform application to the front and back of the leaves (the amount applied was such that the solution did not drip onto the leaves). After application, the test seedlings were cut at a point 10 cm from the leaf end of the stem, and moist hydroponic cotton (a layer of blow-molded paper was attached to the top of the hydroponic cotton to prevent insects from falling onto the cotton and affecting the test results) was fixed in a 25 mL plastic cup. Water was added to the cup, and finally, the treated test seedlings were placed in a circular plastic box, lined with 120 mesh gauze, sealed with a perforated lid, and cultured at 25°C.

[0112] 48 hours after application, the insect was lightly touched with a small brush; if there was no reaction, it was considered dead. The control effect was calculated using the following formula.

[0113] Population density reduction rate (%) = Number of dead insects after treatment / Total population density after treatment × 100 Control effectiveness (%) = (Percentage reduction in population density of treated group - Percentage reduction in population density of blank group) / (100 - Percentage reduction in population density of blank group) × 100 Note 1) The total population density after treatment refers to the population density base number (sum of live and dead insects) after treatment, and not the population density base number recorded before treatment (because some thrips are lost when they fly away during the treatment process, the population density base number before treatment becomes larger than the actual treated population density base number).

[0114] JPEG2026093990000001.jpg151170

[0115] JPEG2026093990000002.jpg145170

[0116] JPEG2026093990000003.jpg104170JPEG2026093990000004.jpg45170

[0117] As can be seen from the results in Tables 2 and 3, ginger plant extracts are more effective overall against adult false spider mites compared to adult thrips. Ethanol extract from ginger rhizome showed the best effect against false spider mites and adult thrips, and ethanol extract from turmeric rhizome also showed a similarly significant insect-suppressing effect. This phenomenon was also observed for false spider mite eggs and thrips eggs, with the effects of these two extracts being the most pronounced. This indicates that the main substance that suppresses insects and their eggs is in the ethanol extract. In terms of repellent activity, the activity of volatile oils was significantly higher than that of the extracts. This is because volatile oils contain many volatile substances, and the repellent activity of the rhizome volatile oils of these two ginger plants may be superior to that of the whole plant volatile oil. Furthermore, turmeric rhizome volatile oil showed a significant repellent effect against adult thrips, and ginger rhizome ethanol extract showed a significant repellent effect against thrips.

[0118] Example 2 The rhizomes of ginger were sliced, dried in a 60°C oven for 24 hours, and then crushed and sieved through a 40-mesh sieve to obtain a coarse powder. 100g of the coarse ginger powder was taken and placed in a reflux apparatus. Ethanol, ethyl acetate, or an ethanol-ethyl acetate mixed solvent (in ratios of 1:1, 2:1, and 4:1) was added, and the reflux apparatus was started. Reflux extraction was performed until the solvent was gone, followed by filtration and concentration. The solvent-to-liquid ratio was 1:40, the extraction time was 2 hours, and the extraction was performed twice. The above extracts were diluted to lower concentrations according to the bioassay method in Example 1, and their control activity against adult false spider mites was observed.

[0119] JPEG2026093990000005.jpg59170

[0120] As can be seen from the results in Table 4, the control effect against pests decreases after reducing the concentration of the extract. However, at the same concentration, the extract extracted with a mixed solvent of ethanol and ethyl acetate has a higher pest control effect than the extract extracted with a single solvent. Furthermore, when the mixing ratio of ethanol to ethyl acetate is 4:1, an 80% control effect is obtained at a concentration of 1 mg / mL, making it a preferred solvent.

[0121] Example 3 (Extraction method using ginger solvent) The rhizomes of ginger were sliced, dried in a 60°C oven for 24 hours, crushed, and then sieved through a 40-mesh sieve to obtain a coarse powder. 100g of the coarse ginger powder was taken and placed in a reflux apparatus. A mixture of two solvents, ethyl acetate and ethanol, was added, and the reflux apparatus was started. The mixture was refluxed until the solvent was gone, then filtered and concentrated to obtain a ginger extract.

[0122] The extraction conditions were: liquid-to-liquid ratio of 1:40, extraction time of 2 hours, solvent ratio (ethyl acetate:ethanol = 1:4), and number of extractions of 3.

[0123] (Extraction method using turmeric solvent) The rhizomes of the turmeric plant were sliced, dried in a 60°C oven for 24 hours, crushed, and then sieved through a 40-mesh sieve to obtain a coarse powder. 100g of the coarse turmeric powder was taken and placed in a reflux apparatus. A mixture of two solvents, ethyl acetate and ethanol, was added, and the reflux apparatus was started. The extraction was performed under reflux until the solvent was gone, followed by filtration and concentration to obtain a turmeric extract.

[0124] The extraction conditions were: liquid-to-acid ratio of 1:20, extraction time of 4 hours, solvent ratio (ethyl acetate:ethanol = 1:4), and number of extractions of 2.

[0125] (Method for extracting ginger oil) The rhizomes of ginger were sliced, dried in a 60°C oven for 24 hours, crushed, and then sieved through a 40-mesh sieve to obtain a coarse powder. 500g of the coarse ginger powder was placed in a gasoline apparatus, a specific amount of sodium chloride-containing aqueous solution was added, the heating apparatus was started, and distillation was carried out for 3-10 hours to obtain ginger gasoline.

[0126] The extraction conditions were a liquid-to-liquid ratio of 1:20, a distillation time of 5 hours, and a sodium chloride dose of 2%.

[0127] (Method for extracting turmeric volatile oil) The rhizomes of the turmeric plant were sliced, dried in a 60°C oven for 24 hours, crushed, and then sieved through a 40-mesh sieve to obtain a coarse powder. 500g of the coarse turmeric powder was placed in a gasoline apparatus, a specific amount of sodium chloride-containing aqueous solution was added, the heating apparatus was started, and distillation was carried out for 3-10 hours to obtain turmeric gasoline.

[0128] The extraction conditions were a liquid-to-liquid ratio of 1:20, a distillation time of 5 hours, and a sodium chloride dose of 2%.

[0129] Example 4 According to the optimal extraction conditions for ginger extract, ginger oil, turmeric extract, and turmeric oil prepared in Example 3, the corresponding extracts were prepared. A blending ratio selection test of adult false spider mites and eggs was performed using the bioassay method of Example 1. JPEG2026093990000006.jpg172170

[0130] As can be seen from Table 5, the insecticidal activity is highest when the ratio of ginger extract to turmeric volatile oil is 7:3.

[0131] JPEG2026093990000007.jpg180170

[0132] As can be seen from Table 6, the ovicidal activity exceeded 80% in both the case where the ginger extract and turmeric oil ratio was 7:3 and the case where the ginger extract and turmeric oil ratio was 3:7. However, the acaricidal activity in the case where the ginger extract and turmeric oil ratio was 3:7 was not particularly remarkable. Overall, the overall activity was highest when the ginger extract and turmeric oil ratio was 7:3.

[0133] Example 5 A ginger and turmeric mixture was prepared using the extraction conditions of Example 3, with a mixing ratio of ginger extract to turmeric volatile oil of 7:3, and verified using the test method of Example 1. The results are shown in the table below.

[0134] JPEG2026093990000008.jpg31170

[0135] Example 6 Using the extraction conditions of Example 3, a mixture of ginger extract and turmeric oil was prepared with a mixing ratio of 7:3, and the activity of beet armyworm, tobacco whitefly adults and eggs was measured according to the test method of Example 1.

[0136] (Spodoptera litura egg test) The egg masses were finely chopped, with approximately 60 eggs per mass. They were immersed for 10 minutes in a test solution prepared together with ovipositing paper. After removal, excess solution adhering to the ovipositing paper and egg masses was absorbed and removed using absorbent paper. Each egg mass was placed in a glass test tube (5.0 cm high, 2.5 cm in diameter, the same applies below), sealed with plastic paper punctured with an insect pin, and cultured in an artificial climate box at (24±1)℃, relative humidity (80±10)%, and light-dark cycle L:D=12:12. When the eggs had developed to the point of almost hatching, castor leaves approximately 3 cm in diameter were added and fed to the hatched larvae. The number of hatched and unhatched eggs in each egg mass was inspected and recorded, and the egg mortality rate and calibration mortality rate were calculated using a formula. Each treatment was repeated three times. The test materials in each treatment group were periodically moistened, and the hatching status of the eggs was observed. On the fourth day after administration, the number of hatched eggs for each treatment was recorded, and the results were recorded in a logbook. The investigation time may be shortened or extended depending on the test requirements and the characteristics of the drug.

[0137] Egg hatching rate (%) = Number of hatched eggs / Total number of processed eggs * 100 Control effectiveness (%) = (Egg hatching rate in control group - Egg hatching rate in treated group) / Egg hatching rate in control group) * 100

[0138] (Spodoptera litura larvae test) Fresh, clean tobacco leaves were cut, and small, round leaves with a diameter of 2.5 cm were punched out using a round punch. Twelve leaves were selected as a group, spread out, wrapped in gauze, or held directly with tweezers, and immersed in the test solution. After immersion in the prepared solution for 10 seconds, the leaves were removed and excess solution on the gauze was absorbed and removed with filter paper. First, a blank control was processed, and then the above procedure was repeated in an order from low to high according to the test dose, with the gauze and absorbent paper replaced between each treatment, and tools such as tweezers being cleaned. The leaves after immersion in the solution were placed on filter paper, spread out at 25±1℃, and allowed to air dry. Fifteen third-instar beet armyworm larvae were selected, wrapped in gauze, and each was immersed in the prepared solution for 10 seconds. After removal, excess solution on the gauze was removed with filter paper. First, a blank control was treated, and then the above procedure was repeated in order from low to high according to the test dose, with each treatment repeated at least three times. Between treatments, the gauze and absorbent paper were replaced, and tools such as tweezers were cleaned. If there were no obvious water droplets or dampness on the leaf surface, four chemically treated leaves and 15 chemically treated Spodoptera litura larvae were placed in a culture dish. The culture dish was reared and observed under normal conditions of 25±1℃ and 60-80% humidity. Depending on the test requirements and the characteristics of the chemical, the response of the test insects was observed at regular intervals, and clean leaves or feed were replenished. 72 hours after application, a regular check was performed, and the number of dead Spodoptera litura larvae for each treatment was recorded. The criterion for determining insect death was to lightly touch the insect with tweezers; if there was no reaction, it was considered dead. The control effect was calculated using the following formula.

[0139] Population density reduction rate (%) = (Base population density of the treated group - Number of worms after treatment in the treated group) / Total population density after treatment in the treated group × 100 Control effectiveness (%) = (Percentage reduction in population density of treated group - Percentage reduction in population density of blank group) / (100 - Percentage reduction in population density of blank group) × 100

[0140] (Tobacco whitefly egg test) Cucumber leaves were collected, and a 2cm diameter circular hole was punched out in the central vein of each leaf using a hole punch. The leaves were then immersed in the test solution for 10 seconds, removed and dried, and then placed in finger-shaped tubes containing 12g / L agar solution (with the front of the leaf facing downwards). 30 adult insects were placed in these tubes and allowed to lay eggs for 24 hours. After the adults were removed, the leaves containing the eggs were transferred to culture dishes containing 12g / L agar solution (with the front of the leaf facing downwards), and the number of eggs was statistically counted under a microscope. Subsequently, the cultures were observed in an illuminated incubator [temperature (27±1)℃, humidity (70±5)%, light-dark cycle 16h:8h]. After 5 days, the number of unhatched eggs was counted under a microscope, and the egg hatching inhibition rate was calculated. Each treatment was repeated three times.

[0141] Egg hatching rate (%) = Number of hatched eggs / Total number of processed eggs * 100 Control effectiveness (%) = (Egg hatching rate in control group - Egg hatching rate in treated group) / Egg hatching rate in control group) * 100

[0142] (Tobacco whitefly adult test) A double-pass glass tube (3 cm in diameter, 6 cm in length) was taken, wrapped with one layer of opaque black cellophane, and covered with one layer of Parafilm (the film area was uniformly stretched to 4-5 times its original size) at one end. 200 μL of the test drug solution was applied to the wall of the double-pass glass tube, and the solution was uniformly distributed to the tube wall by rotating the double-pass glass tube once. After the solution evaporated, 200 μL of 30% sucrose water was added to the Parafilm on the wall of the drug-containing container, and another layer of Parafilm was placed on top to prevent evaporation of the substrate. Thirty 2-5 day instar adult tobacco whiteflies were placed in the other end of the double-pass glass tube, and the end was sealed with absorbent paper. This was repeated three times for each group, and the tubes were placed in an artificial climate chamber (25 ± 3°C, 60%-70% humidity, light-dark ratio = 14h:10h). After 48 hours, the number of viable insects was examined and recorded under a stereomicroscope.

[0143] Population density reduction rate (%) = (Base population density of the treated group - Number of worms after treatment in the treated group) / Total population density after treatment in the treated group × 100 Control effectiveness (%) = (Percentage reduction in population density of treated group - Percentage reduction in population density of blank group) / (100 - Percentage reduction in population density of blank group) × 100

[0144] JPEG2026093990000009.jpg43170

[0145] As can be seen from the data in Examples 5 and 6, even when using the same insecticide, different control effects are observed against different pests. By using the ginger and turmeric mixture of the present invention, an extremely high control effect against agricultural mites can be achieved at a low concentration (1 mg / mL). However, for beet armyworms, tobacco whiteflies, etc., the control effect is relatively low even at high concentrations of 2 and 4 mg / mL. Therefore, the composition of the present invention is particularly applicable to the control of agricultural mites.

[0146] Example 7 A mixture of ginger extract and turmeric oil was prepared using the extraction conditions of Example 3, with a blending ratio of 7:3. A 10% soluble liquid formulation of the ginger-turmeric mixture (by mass) was prepared according to the following formulation, and a field test for citrus red mites was conducted. Mixture 10%, ethanol 20%, calcium dodecylbenzenesulfonate 2%, castor oil ethoxy compound 2%, tristyrylphenol polyoxyethylene ether 1%, ethylene glycol 5%, the remainder being water.

[0147] (Field trial of citrus red mite) The experiment was conducted on September 30, 2024, in Dacao Village, Shou'an County, Sichuan Province. The experiment consisted of four treatments, each using two citrus trees. After preparing the pesticide according to the test design, it was sprayed evenly onto the leaves and both sides of the fruit of the citrus trees in each area until small droplets formed. It was also necessary to ensure that the main trunk and other growing parts of the fruit trees were in contact with the pesticide, and no other insecticides or fungicides were used during the test period. Before application, six markers were placed in each area, and the population density cardinality at each marker was statistically determined. Each marker contained at least 15 adult mites and at least 30 nymphs. After application, the number of mites remaining on the leaves during each survey was statistically determined. The population density cardinality was surveyed before application, and the number of remaining mites was surveyed on days 1, 7, and 10 after application, for a total of three surveys.

[0148] Population density reduction rate (%) = [(Population density before treatment - Population density after treatment) / Population density before treatment] * 100 Control effectiveness (%) = (Percentage reduction in population density in the treated area - Percentage reduction in population density in the control area) / (1 - Percentage reduction in population density in the control area) * 100

[0149] JPEG2026093990000010.jpg122170JPEG2026093990000011.jpg29170

[0150] Example 8 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:40, an extraction time of 2 hours, a solvent ratio (ethyl acetate:ethanol = 1:1), and two extractions.

[0151] Example 9 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:40, an extraction time of 4 hours, a solvent ratio (ethyl acetate:ethanol = 1:1), and two extractions.

[0152] Example 10 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:40, an extraction time of 2 hours, a solvent ratio (ethyl acetate:ethanol = 1:1), and three extractions.

[0153] Example 11 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:40, an extraction time of 2 hours, a solvent ratio (ethyl acetate:ethanol = 1:1), and three extractions.

[0154] Example 12 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:20, an extraction time of 2 hours, a solvent ratio (ethyl acetate:ethanol = 1:1), and three extractions.

[0155] Example 13 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:60, an extraction time of 2 hours, a solvent ratio (ethyl acetate:ethanol = 1:1), and three extractions.

[0156] Example 14 (Extraction method for ginger and turmeric) Referring to Example 2, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-water ratio of 1:40, an extraction time of 2 hours, a solvent ratio (ethyl acetate:ethanol = 1:2), and three extractions.

[0157] Example 15 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:40, a distillation time of 5 hours, and a sodium chloride dose of 2%.

[0158] Example 16 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:60, a distillation time of 5 hours, and a sodium chloride dose of 2%.

[0159] Example 17 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:20, a distillation time of 3 hours, and a sodium chloride dose of 2%.

[0160] Example 18 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:20, a distillation time of 7 hours, and a sodium chloride dose of 2%.

[0161] Example 19 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:20, a distillation time of 5 hours, and a sodium chloride dose of 4%.

[0162] Example 20 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:20, a distillation time of 5 hours, and a sodium chloride dose of 6%.

[0163] Example 21 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:40, a distillation time of 3 hours, and a sodium chloride dose of 2%.

[0164] Example 22 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:40, a distillation time of 7 hours, and a sodium chloride dose of 2%.

[0165] Example 23 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-liquid ratio of 1:40, a distillation time of 7 hours, and a sodium chloride dose of 4%.

[0166] Example 24 (Method for extracting ginger and turmeric volatile oils) Referring to Example 3, ginger rhizomes and / or turmeric rhizomes were collected. The extraction conditions were a liquid-to-powder ratio of 1:40, a distillation time of 7 hours, and a sodium chloride dose of 6%.

[0167] Example 25 (Component analysis of the extract) The optimal extract combination for Example 4 (ginger extract: turmeric volatile oil = 7:3) was fully scanned by gas-phase tandem mass spectrometry and compared with existing databases. The specific conditions were: HP-5MS column, capillary column (30m × 250μm × 0.25μm); column temperature 60°C, programmed heating: 60°C, hold for 1 min, 40~120°C, hold for 0 min, 5~310°C, hold for 0 min, operation for 40.5 min, inlet temperature 280°C, auxiliary heater 280°C; no flow splitting, ion source temperature 280°C, scanning type MS2 scanning.

[0168] JPEG2026093990000012.jpg221170 Note: The compounds in the table above are compared and analyzed using the Agilent NIST MS Search 2.3 search program.

[0169] Example 26 A mixture of ginger extract and turmeric oil was prepared by mixing them in a 7:3 ratio under the extraction conditions of Example 3. This mixture was then combined with α-zingiberene, zingerone, α-curcumene, 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone, and 1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl) sulfite (all with a purity exceeding 85%), which were analyzed and identified in the above examples. The control effect against adult false spider mites was investigated using the test method of Example 1.

[0170] Based on the proposed concept of independent combined action, Bliss believes that the theoretical mortality rate P when insecticides and acaricides are mixed can be calculated using the following formula. P = Pm + Pn(1 - Pm) Pm represents the target mortality rate (%) when the concentration of the first active ingredient is m, and Pn represents the target mortality rate (%) when the concentration of the second active ingredient is n.

[0171] If, after mixing two active ingredients at specific concentrations, the actual mortality rate of the target is higher than the theoretical mortality rate P, then the mixed use of the two active ingredients at predetermined concentrations has a synergistic effect; conversely, if the opposite is true, then it has an antagonistic effect.

[0172] JPEG2026093990000013.jpg176170

[0173] As can be seen from the table above, when α-curcumene, 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone, and 1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl) sulfite are combined with a mixture of ginger and turmeric, they have a synergistic effect in controlling adult false spider mites. However, when α-zingiberene and gingerone are combined with a mixture of ginger and turmeric, there is no synergistic effect.

Claims

1. The use of ginger plant extracts in the killing and / or control of pests, and / or extermination of pests, and / or prevention or reduction of pest oviposition, and / or prevention or reduction of pest egg hatching, The ginger plant is one or more selected from ginger, turmeric, galangal, hepatica, and ginger lily; the extract is one or more selected from volatile oil, water extract, ethanol extract, ethyl acetate extract, and methanol extract; the extract can be obtained by extracting from one ginger plant or from two or more ginger plants; and the pest is selected from thrips, and / or spider mites, and / or gall mites, and / or dust mites, and / or louse mites, and / or green mites, and / or predatory mites, and / or scabiidae, and / or dust mites, and / or dust mite mites.

2. The aforementioned pests include the flat-headed thrips (Frankliniella intonsa), the onion thrips (Thrips tabaci Lindeman), the bean thrips (Taeniothrips distalis Karny), the rice thrips (Stenchaetothrips biformis), the flower thrips (Thrips hawaiiensis Morgan), the southern yellow thrips (Thrips palmi Karny), the citrus yellow thrips (Frankliniella occidentalis), and the loquat thrips (Thrips japonicus). Bagnall), sugarcane thrips (Thrips serratus Kobus), cajonka nose thrips (Frankliniella tenuicornis Uzel), tea yellow thrips (Scirtothrips dorsalis Hood), croton thrips (Heliothrips haemorrhoidalis Bouche), tea yellow thrips (Scirtothrips dorsalis Hood), Scolothrips sexmaculatus Pergandé, Oligonychus baipisongis, Oligonychus karamatus, Oligonychus rubicundus, Eotetranychus albus, Eotetranychus bailae, Eotetranychus camelliae, Tetranychus neocaledonicus, Tetranychus phaselus, Tetranychus (Tetranychus cinnabarinus), Schizotetranychus baltazarae, Schizotetranychus bambasae, Schizotetranychus elongatas)elongatus), Mixonychus (Bakerina) aestiva, Mixonychus ganjuis, Panonychus citri, Panonychus caglei, Allonychus bambasae, Allonychus wuyinicus, Stigmaeopsis celerius, Mononychellus georgicus, Acanthonychus jampengensis The use according to claim 1, characterized in that it is one or more species selected from (jiangfengensis), cherry spider mite (Amphitetranychus viennenensis), scabies mite, flour mite, and dust mite.

3. The use according to claim 1, characterized in that the ginger plant extract is one selected from ginger rhizome extract and turmeric rhizome extract, or a mixture of ginger rhizome extract and turmeric rhizome extract in a ratio of 1 to 9:9 to 1.

4. An insecticide composition comprising a mixture of ginger rhizome extract and turmeric rhizome extract, wherein the ratio of ginger rhizome extract to turmeric rhizome extract is 1 to 9:9 to 1, the ginger rhizome extract is extracted from ginger rhizome with ethanol:ethyl acetate in a ratio of 1 to 4:1, and the turmeric rhizome extract is volatile oil of turmeric rhizome.

5. The insecticidal composition according to claim 4, characterized in that the mixing ratio of ginger rhizome extract and turmeric rhizome extract is 7 to 9:3 to 1.

6. The insecticidal composition according to claim 4, characterized in that the ratio of ginger rhizome extract to turmeric rhizome extract is 7:

3.

7. The insecticidal composition according to claim 4, characterized in that the mixture of ginger rhizome extract and turmeric rhizome extract is 0.1 to 100% in the composition.

8. The insecticidal composition according to claim 4, further comprising a solvent, wherein the total concentration of the mixture of ginger rhizome extract and turmeric rhizome extract is 0.1 mg / mL or more.

9. The insecticidal composition according to claim 4, further comprising a solvent, wherein the total concentration of the mixture of ginger rhizome extract and turmeric rhizome extract is 0.1 to 1000 mg / mL.

10. The insecticidal composition according to claim 4, characterized in that, in addition to the mixture, it further comprises one or more of α-curcumene, 5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone, and 1-(2,4-dichlorophenoxy)propan-2-yl(2-fluoroethyl)sulfite.

11. The insecticidal composition according to claim 4, characterized in that the dosage form of the composition includes a soluble powder, a soluble granule, a soluble liquid, a dispersible liquid, an aqueous emulsion, a microemulsion, a microcapsule suspension, a seed treatment liquid, and an aerosol.

12. A method for killing and / or controlling pests, and / or eradicating pests, and / or preventing or reducing pest oviposition, and / or preventing or reducing the hatching of pest eggs, A method characterized by exposing pests and / or pest eggs to an insecticidal composition according to any one of claims 4 to 11.

13. The method according to claim 12, characterized in that the pest is one or more species selected from the following: Thyropterans, Hemiptera, Lepidoptera, Spider mites, Lesser spider mites, Gall mites, Dust mites, Lice mites, Claw mites, Cheyletiidae, Sarcoptes scabiei, Dust mites, and Dust mites.

14. The aforementioned pests include: flat-headed thrips, onion thrips, bean thrips, rice thrips, flower thrips, southern yellow thrips, citrus yellow thrips, loquat thrips, sugarcane thrips, cajonka thrips, tea yellow thrips, croton thrips, tea yellow thrips, Scorotripsy sexgumaculatus, rice leaf borer (Cnaphalocrocis medinalis), white-spotted armyworm (Spodoptera exigua), beet armyworm (Spodoptera litura), and peach fruit borer (Carposina). sasakii, Helicoverpa armigera, Plutella xylostella, Diaphania indica, Maruca testularis Geyer, Bemisia tabaci Gennadius, Trialeurodes vaporariorum, Aleurocanthus spiniferus, Dialeurodes citri Ashm, Bemisia myricae Kuwana), Aleurocybotus indicus, Pyraling whitefly (Aleurodicus dispersus), Oligonyx baipisongis, Larch spider mite, Japanese spider mite, Eotetranicus albus, Eotetranicus bailaye, Camellia spider mite, Southern spider mite, Sagami spider mite, Two-spotted spider mite, False two-spotted spider mite, Ryujin spider mite, Bamboo spider mite, Schizotetranyx elongatas, Mixonyx (Bacherina) aesteva, Mixonyx ganjuis, Citrus spider mite The method according to claim 12, characterized in that the insects are selected from Pannonius cagley, Arronius bambusae, Arronius winicus, bamboo dwarf mite, Mononicerus georgicus, Acanthonius jampengensis, cherry mites, pear psyllids, leafhoppers (Cicadellidae), Liliomiza, lacewings, fire ants, termites, codling moths, mosquitoes, flies, cockroaches, and fleas.

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