Temperature-responsive beads and method for manufacturing the same

Abstract

The objective is to provide temperature-responsive beads with minimal defects on the coated carrier surface. Furthermore, the objective is to provide a method for manufacturing temperature-responsive beads suitable for industrialization. [Solution] The present invention provides temperature-responsive beads comprising a coating agent containing at least a temperature-responsive polymer having a lower critical dissolution temperature (LCST) in the range of 0°C to 50°C, and a support, wherein the beads consist of a group of beads in which 10% or less of the total number of beads have defects.

Claims

1. A temperature-responsive bead comprising a coating agent containing at least a temperature-responsive polymer having a lower critical dissolution temperature (LCST) in the range of 0°C to 50°C, and a support, wherein the bead group consists of beads in which 10% or less of the total number of beads have defects.

2. A temperature-responsive bead comprising a coating agent containing at least a temperature-responsive polymer having a lower critical dissolution temperature (LCST) in the range of 0°C to 50°C, and a support, manufactured using a fluidized bed coating method.

3. The temperature-responsive beads according to claim 1 or 2, wherein the temperature-responsive polymer is a block copolymer comprising the blocks (A), (B), and (C) below; (A) Polymer blocks with HLB values ​​(Griffin method) in the range of 7 to 20 (B) Polymer blocks with HLB values ​​(Griffin method) in the range of 0 or more and less than 7 (C) A temperature-responsive polymer block in which the LCST is in the range of 0°C to 50°C.

4. The temperature-responsive beads according to claim 1 or 2, wherein the temperature-responsive polymer comprises a block polymer of 2-methoxyethyl acrylate, n-butyl acrylate, and N-isopropylacrylamide.

5. The temperature-responsive beads according to claim 1 or 2, wherein the temperature-responsive polymer is a block copolymer comprising the blocks (B) and (C) below; (B) Polymer blocks with HLB values ​​(Griffin method) in the range of 0 or more and less than 7 (C) A temperature-responsive polymer block in which the LCST is in the range of 0°C to 50°C.

6. The temperature-responsive beads according to claim 1 or 2, wherein the temperature-responsive polymer comprises a block polymer of n-butyl methacrylate and N-isopropylacrylamide.

7. The temperature-responsive beads according to claim 1 or 2, further comprising a copolymer polymer of p-carboxystyrene and styrene in the coating agent.

8. The temperature-responsive beads according to claim 1 or 2, wherein the coating thickness is 10 nm to 150 nm.

9. The temperature-responsive beads according to claim 1 or 2, wherein the average particle size of the carrier is 50 μm or more and 1000 μm or less.

10. A method for producing a temperature-responsive polymer, comprising the steps of producing temperature-responsive beads using a fluidized bed coating method, wherein the temperature-responsive beads consist of a group of beads in which 10% or less of the total number of beads are defective, and the coating agent and support contain at least a temperature-responsive polymer having an LCST in the range of 0°C to 50°C, and the beads are composed of 10% or less of the total number of beads.

11. A method for producing temperature-responsive beads, comprising the step of producing temperature-responsive beads, which include a coating agent containing at least a temperature-responsive polymer having an LCST in the range of 0°C to 50°C, and a support, using a fluidized bed coating method.

12. A method for culturing cells using temperature-responsive beads according to claim 1 or 2, or temperature-responsive beads manufactured by the method described in claim 10 or 11.

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