Method for purifying fusion proteins containing an IgG Fc domain
The method of multimodal and cation exchange chromatography addresses inefficiencies in purifying fusion proteins with an IgG Fc domain, achieving low endotoxin levels and high purity through a streamlined process.
JP2026094217APending Publication Date: 2026-06-09ALTEOGEN INC
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- ALTEOGEN INC
- Filing Date
- 2026-02-20
- Publication Date
- 2026-06-09
AI Technical Summary
Technical Problem
Existing methods for purifying fusion proteins with an IgG Fc domain are inefficient in removing endotoxins and achieving high purity, particularly in large-scale production.
Method used
A method involving multimodal chromatography and cation exchange chromatography without anion exchange, including steps of culturing, primary chromatography, and subsequent purification using multimodal and cation exchange chromatography, to achieve endotoxin levels below 0.05 EU/mg and size exclusion HPLC purity of 98% or higher.
Benefits of technology
Enables efficient and large-scale production of high-purity fusion proteins with an IgG Fc domain, effectively removing impurities and maintaining protein integrity.
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Abstract
This invention provides a method for effectively purifying fusion proteins containing an IgG Fc domain. [Solution] A method for purifying a fusion protein having an IgG Fc domain, which yields a fusion protein having an IgG Fc domain with endotoxin <0.05 EU / mg or a size exclusion HPLC purity of 98% or higher. The method comprises the steps of: a) culturing cells that produce a fusion protein having an IgG Fc domain; b) recovering the protein from the cells cultured in a); c) purifying the protein recovered in b) by primary chromatography; and d) purifying the protein purified in c) by primary chromatography by multimodal chromatography and cation exchange chromatography, without using anion exchange chromatography.
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