Microbial protein hydrolysates, methods for preparing them, and their uses.
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- AIR PROTEIN INC
- Filing Date
- 2026-03-17
- Publication Date
- 2026-06-23
AI Technical Summary
【0004】 動物細胞培養または乳酸菌(LAB)培養などの細胞培養物の生育に、蛋白質加水分解物が有益な効果を与えることは周知であり、何十年もの間、有用な細胞培養添加剤として利用されてきた。乳酸菌(LAB)は、アミノ酸合成能に関しては限定的であるため、アミノ酸およびペプチドの外来供給源に依存する。したがって、乳酸菌(LAB)培養における増殖にとって、生化学的窒素源(例えば、蛋白質、ペプチドおよびアミノ酸)の提供は必須である。典型的なアミノ酸源は、乳汁およびその他の加水分解蛋白質中のペプチドである。様々な由来の蛋白質加水分解物が、各種の乳酸菌(LAB)株に異なる効果をもたらすことが知られており、菌増殖を支援するものもあれば、あるいは特定種類のアミノ酸代謝を誘導するものもある。乳酸菌(LAB)の発酵において良好に用いられることが報告されている異なる市販の蛋白質加水分解産物としては、N-Zアミン、Hy-Case、Hy-Soy、Edamin、およびN-Z-Caseが挙げられる(Misono, H., Goto, N., & Nagasaki, S. (1985). Purification, crystallization and properties of nadp+-specific glutamate dehydrogenase from lactobacillus fermentum. Agricultural and Biological Chemistry. https://doi.org/10.1080/00021369.1985.10866676; Molskness, T.A., Lee, D.R., Sandine, W.E., & Elliker, P. R. (1973). -D-phosphogalactoside galactohydrolase of lactic streptococci. Applied Microbiology. https://doi.org/10.1128/aem.25.3.373-380.1973; Viniegra-Gonzalez, G., & Gomez, J. (1984). Lactic acid production by pure and mixed bacterial cultures. Bioconversion Systems. Boca Raton, FL; CRC Press. P, 17-39.; Vedamuthu, E.R. (1980). Method for diacetyl flavor and aroma development in creamed cottage cheese. 米国特許第4,191,782号.; Kegel, M.A., & Wallace, D.L. (1989). Use of stabilizing agents in culture media for growing acid producing bacteria. 米国特許第4,806,479号)。
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Abstract
Claims
1. A method for producing a culture medium for culturing microorganisms or cells, wherein the method is: The method involves cultivating a first microorganism in a culture medium containing a porous scaffold, thereby producing biomass. Here, the porous scaffold contains polyhydroxyalkanoic acid (PHA), and the first microorganism contains a chemoautotrophic microorganism. To cultivate a first microorganism and thereby produce biomass; Processing the biomass produced by the first microorganism in order to produce a protein hydrolysate composition containing the biomass and the porous scaffold; and Adding the protein hydrolysate composition to a culture medium for a second culture comprising a second microorganism or cells, Here, the protein hydrolysate composition serves as a nutrient source for the growth of the second microorganism or cell. Adding the protein hydrolysate composition to the culture medium, Methods that include...
2. A method according to claim 1, wherein the treatment comprises treating the biomass with an increase in temperature, an increase in pressure, a protease, or a combination thereof.
3. The method according to claim 1, wherein the processing is: (i) Raise the pH of the suspension containing the biomass; or (ii) Lowering the pH of the suspension containing the biomass, Methods that include...
4. A method according to claim 1, wherein the PHA comprises polyhydroxybutyric acid (PHB).
5. A method according to claim 1, wherein the protein hydrolysate composition contains a vitamin.
6. The method according to claim 4, wherein the vitamin is vitamin B １ Vitamin B ２ , or vitamin B １２ A method that includes one or more of the following types.
7. The method according to claim 1, wherein the second culture comprises animal cells.
8. The method according to claim 6, wherein the animal cells are in a serum-free medium.
9. The method according to claim 6, wherein the animal cells are in a culture medium free of animal components.
10. A method according to claim 1, wherein the protein hydrolysate composition is used as an additive for culturing recombinant animal cells.
11. A method according to claim 1, wherein the protein hydrolysate composition is used as an additive for culturing meat.
12. A method according to claim 1, wherein the chemoautotrophic microorganism is cultured under independent inorganic nutrient conditions.
13. A method according to claim 11, wherein the independent inorganic nutrient conditions include providing a gaseous substrate for growing the chemoautotrophic microorganism.
14. A method of claim 12, wherein the gaseous substrate comprises a carbon source, and the carbon source is CO ２ , CO, or CH ４ A method that includes one or more of the following types.
15. A method of claim 12, wherein the gaseous substrate comprises an electron donor, and the electron donor is H ２ , CO, or CH ４ A method that includes one or more of the following types.
16. A method according to claim 12, wherein the gaseous substrate includes a pyrolysis gas, a generator gas, a synthesis gas, a natural gas, or a biogas.
17. The method according to claim 1, wherein the chemoautotrophic microorganism is a hydrogen-oxidizing microorganism.
18. The method of claim 1, wherein the chemoautotrophic microorganism is: Aquifex species; Cupriavidus species; Corynebacterium species; Gordonia species; Nocardia species; Rhodopseudomonas species; Rhodobacter species; Rhodospirillum species; Rhodococcus species; Rhizobium species; Thiocapsa species; Pseudo Species of *Onas*; species of *Hydrogenomonas*; species of *Hydrogenobacter*; species of *Hydrogenophilus*; species of *Hydrogenovibrio*; species of *Hydrogenothermus*; species of *Helicobacter*; species of *Xanthobacter*; species of *Hydrogenophaga*; species of *Bradyrhizobium*; species of *Ralstonia*; species of *Alcaligenes* ; Amycolata species; Aquaspirillum species; Arthrobacter species; Azospirillum species; Variovorax species; Acidovorax species; Bacillus species; Calderobactenum species; Derxia species; Flavobacterium species; Microcyclous species; Mycobacterium species; Paracoccus species; Pe A method selected from the group consisting of species of rsephonella; species of Renobacter; species of Seliberia; species of Streptomycetes; species of Thermocrinis; species of Wautersia; species of Anabaena; species of Arthrospira; species of Scenedesmus; species of Chlamydomonas; species of Ankistrodesmus; and species of Rhaphidium.
19. A method according to claim 1, wherein the chemoautotrophic microorganism is genetically modified.
20. A method according to claim 1, wherein the porous scaffold induces the differentiation of the first microorganism.