Density gradient formation using sample particles

The system automates the formation of density gradients without centrifugation, reducing separation time and enhancing throughput by directly supplying gradients with controlled density variations, addressing the inefficiencies of traditional methods.

JP2026510215APending Publication Date: 2026-04-02BECKMAN COULTER INC
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Patent Information

Application Number
JP2025546220
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2023-02-15
Filing Date
2024-02-13
Publication Date
2026-04-02

AI Technical Summary

Technical Problem

Existing methods for separating particles such as virus vectors, extracellular vesicles, and nucleic acids using density gradients are time-consuming due to the need for centrifugation to form the gradient and the time required for particles to reach their equilibrium positions.

Method used

A system and method for forming a density gradient without centrifugation by automatically supplying a density gradient into a container with sample particles, using a processing network to pump and mix components, and controlling the density variation within the gradient to reduce centrifugation time.

Benefits of technology

Significantly reduces the time required to form and reach equilibrium in the density gradient, allowing for faster separation of particles with high resolution, especially in large-scale and small-scale workflows, and enables the use of a wider range of density modifiers.

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Abstract

A system for supplying a density gradient into a container is described. The system pumps a volume of sample particles and a density modifier into a mixing chamber that is in fluid communication with the proximal end of the probe. The mixing chamber mixes the sample particles and the density modifier together to form at least a partial density gradient. The system supplies the density gradient into the container through the distal end of the probe. The density gradient has a variation in density between the first and second ends, and at least a portion of the density gradient between the first and second ends includes the supplied volume of sample particles.
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