Polypeptides for inducing tolerance to factor VIII

Abstract

The present invention relates to a polypeptide comprising (i) a VWF portion and (ii) an erythrocyte-binding portion, wherein the polypeptide is capable of binding to blood coagulation factor VIII (FVIII). The erythrocyte-binding portion may be selected from the group consisting of a peptide ligand, an antibody, an antibody fragment, and a single-chain antigen-binding domain (scFv). The polypeptide may be provided as a heterodimer. The polypeptide of the present invention is useful for inducing tolerance to FVIII.

Description

[Technical field]

[0001] The present invention relates to polypeptides useful for reducing the formation of antibodies to Factor VIII. [Background technology]

[0002] Hemophilia A is an inherited bleeding disorder characterized by plasma deficiency of coagulation factor VIII (FVIII). Patients with hemophilia A are currently treated with FVIII replacement therapy. The major complication in 30% of patients is the development of alloantibodies (inhibitors) that can inactivate FVIII activity and render replacement therapy ineffective. Patients with detectable inhibitors are treated with FVIII bypass therapy (FIIa complex concentrate, rFVIIa, emicizumab) and repeated administration of FVIII (ITI) and / or immunosuppressants. Such treatments are costly, require repeated transfusions, and have a success rate of only 60%.

[0003] Immune tolerance is established during the neonatal period and is maintained during adulthood by various physiological mechanisms to prevent immune responses against self-tissues. In hemophilic boys, tolerance to FVIII is not well established because FVIII is not expressed or is misexpressed due to various mutations in the f8 locus, and therefore is not recognized as a self-protein. In adulthood, immune tolerance is induced and maintained by elimination or suppression of antigen-specific B and / or T cells. The main mechanisms involved in this process include antigen presentation by cells specialized in the induction of immune tolerance (macrophages in the liver or spleen), expansion of endogenous regulatory T cells (Tregs), or induction of antigen-specific Tregs.

[0004] The present inventors aimed to formulate and administer FVIII in a way that would actively induce tolerance to FVIII in patients with inhibitors. Non-Patent Document 1 demonstrated that antigens targeted to apoptotic erythrocytes were cleared in the spleen and liver and induced antigen-specific tolerogenic responses in CD4 and CD8 T cells. Glycophorin A is highly expressed on the surface of red blood cells (RBCs) and can be used to target antigens (Ag) to the RBC surface. However, it was found that fusion proteins containing FVIII and an erythrocyte targeting moiety had only limited efficacy in reducing inhibitor formation.

[0005] There is a continuing need in the treatment of hemophilia A to reduce inhibitor formation. [Prior art documents] [Non-patent literature]

[0006] [Non-Patent Document 1] Kontos S. et al., 2012;PNAS;110(1):E60-E68 Summary of the Invention

[0007] The present inventors have surprisingly found that a fusion protein comprising the D'D3 domain of von Willebrand factor (VWF) and an erythrocyte targeting moiety reduces inhibitor formation.

[0008] Accordingly, the present invention relates to the subject matter defined in the following items [1] to

[60] . [1] A polypeptide comprising (i) a VWF portion and (ii) an erythrocyte-binding portion, said polypeptide being capable of binding to blood coagulation factor VIII (FVIII). [2] The polypeptide according to item [1], wherein the VWF portion is capable of binding to FVIII. [3] The polypeptide according to item [1], wherein the VWF portion is VWF or a fragment thereof, preferably human VWF or a fragment thereof. [4] The polypeptide according to any one of the preceding items, wherein the VWF portion comprises the D'D3 domain of VWF. [5] The polypeptide according to any one of the preceding items, wherein the VWF portion comprises or consists essentially of a fragment of VWF. [6] The polypeptide according to any one of the preceding items, wherein the VWF portion consists essentially of a truncated VWF. [7] The polypeptide of any one of the preceding items, wherein the VWF portion comprises an amino acid sequence having at least 90% sequence identity to amino acids 776 to 805 of SEQ ID NO: 18; or an amino acid sequence having at least 90% sequence identity to amino acids 764 to 1242 of SEQ ID NO: 18. [8] The polypeptide of any one of the preceding items, wherein the VWF portion lacks amino acids 1243 to 2813 of SEQ ID NO:18. [9] The polypeptide of any one of the preceding items, wherein the VWF portion consists of (a) amino acids 764 to 1242 of SEQ ID NO: 18, (b) an amino acid sequence having at least 90% sequence identity to amino acids 764 to 1242 of SEQ ID NO: 18, or (c) a fragment of (a) or (b).

[10] The polypeptide according to any one of the preceding items, wherein the VWF portion comprises at least one amino acid substitution compared to the amino acid sequence of wild-type VWF as set forth in SEQ ID NO:18.

[11] The polypeptide according to item

[10] , wherein the at least one amino acid substitution increases the binding affinity of the polypeptide to FVIII compared to a control polypeptide having the same sequence except for the at least one amino acid substitution.

[12] The polypeptide according to item

[10] or

[11] , wherein the at least one amino acid substitution is selected from the group of combinations consisting of S764G / S766Y, S764P / S766I, S764P / S766M, S764V / S766Y, S764E / S766Y, S764Y / S766Y, S764L / S766Y, S764P / S766W, S766W / S806A, S766Y / P769K, S766Y / P769N, S766Y / P769R, S764P / S766L, and S764E / S766Y / V1083A, with reference to the sequence of SEQ ID NO: 18, in terms of amino acid numbering.

[13] The polypeptide according to any one of items

[10] to

[12] , wherein the at least one amino acid substitution is either the combination S764E / S766Y or S764E / S766Y / V1083A.

[14] The polypeptide according to any one of the preceding items, wherein the polypeptide binds to the FVIII with a dissociation constant KD of 1 μM or less.

[15] The polypeptide according to any one of the preceding items, wherein the polypeptide binds to the FVIII with a dissociation constant KD of 1 nM or less.

[16] The polypeptide according to any one of the preceding items, wherein the polypeptide binds to the FVIII with a dissociation constant KD of 0.1 nM or less.

[17] The polypeptide of any one of the preceding items, wherein the polypeptide comprises a half-life extending moiety (HLEM).

[18] The polypeptide according to item

[17] , wherein the HLEM is a heterologous amino acid sequence fused to the VWF portion.

[19] The polypeptide according to item

[18] , wherein the heterologous amino acid sequence comprises or consists of a protein or peptide selected from the group consisting of transferrin and fragments thereof, the C-terminal peptide of human chorionic gonadotropin, an XTEN sequence, homozygous amino acid repeats (HAP), proline-alanine-serine repeats (PAS), albumin, afamin alpha-fetoprotein, vitamin D binding protein, a polypeptide capable of binding to albumin or an immunoglobulin constant region under physiological conditions, neonatal Fc receptor (FcRn), in particular a polypeptide capable of binding to an immunoglobulin constant region and parts thereof, preferably the Fc part of an immunoglobulin, and combinations thereof.

[20] The polypeptide according to item

[17] , wherein the HLEM is conjugated to a polypeptide comprising a VWF portion.

[21] The polypeptide according to item

[20] , wherein the HLEM is conjugated to the C-terminus of the polypeptide comprising the VWF portion.

[22] The polypeptide according to item

[20] or

[21] , wherein the HLEM is selected from the group consisting of hydroxyethyl starch (HES), polyethylene glycol (PEG), polysialic acid (PSA), elastin-like polypeptides, heparosan polymers, hyaluronic acid and non-proteinaceous albumin binding ligands, such as fatty acid chains, and combinations thereof.

[23] The polypeptide according to item

[17] , wherein the HLEM is non-covalently bound to the polypeptide comprising the VWF portion.

[24] The polypeptide according to any one of items [1] to

[20] , wherein the polypeptide does not contain any HLEM conjugated to the polypeptide.

[25] The polypeptide according to any one of the preceding items, wherein the polypeptide is a glycoprotein comprising N-glycans, and preferably at least 75%, preferably at least 85%, of the N-glycans contain, on average, at least one sialic acid moiety.

[26] The polypeptide of any one of the preceding items, wherein the polypeptide exists as a dimer or has at least a high proportion of dimers.

[27] The polypeptide according to item

[26] , wherein at least 50%, or at least 70%, or at least 80%, or at least 90%, or at least 95% of the polypeptide is present as a dimer.

[28] The polypeptide according to item

[26] or

[27] , wherein the two monomers forming the dimer are covalently bound to each other via at least one or more disulfide bridges formed by cysteine ​​residues in the VWF portion.

[29] The polypeptide according to item

[28] , wherein the cysteine ​​residues forming one or more disulfide bridges are selected from the group consisting of Cys-1099, Cys-1142, Cys-1222, Cys-1225, Cys-1227 and combinations thereof, preferably Cys-1099 and Cys-1142, and the amino acid numbering refers to SEQ ID NO: 18.

[30] The polypeptide according to any one of items

[26] to

[29] , wherein the affinity of the dimer for FVIII is greater than the affinity of the monomeric polypeptide for FVIII, and the monomeric polypeptide has the same amino acid sequence as a monomeric subunit of the dimeric polypeptide.

[31] The polypeptide according to any one of items

[26] to

[30] , wherein the dimer:monomer ratio of the polypeptide is at least 1.5, preferably at least 2, more preferably at least 2.5, or at least 3, or at least 4, or at least 5, or at least 10, or at least 20; or the polypeptide does not contain monomeric and / or multimeric forms of the polypeptide; or the polypeptide is essentially free of monomeric and / or multimeric forms of the polypeptide.

[32] The dimeric polypeptide has a dissociation constant K of less than 1 μM, preferably less than 1 nM, more preferably less than 500 pM, less than 200 pM, less than 100 pM, less than 90 pM, or less than 80 pM. DThe polypeptide according to any one of items

[26] to

[31] , having FVIII binding affinity characterized by:

[33] K D is in the range of 0.1 pM to 500 pM, 0.5 pM to 200 pM, 0.75 pM to 100 pM, or most preferably 1 pM to 80 pM.

[34] The polypeptide according to any one of items

[26] to

[33] , wherein the polypeptide is a heterodimer.

[35] The polypeptide according to item

[34] , wherein the heterodimer comprises a first subunit and a second subunit, the first subunit comprising a first VWF moiety and an erythrocyte-binding moiety as defined in any one of the preceding items, and the second subunit comprising a second VWF moiety as defined in any one of the preceding items.

[36] The polypeptide according to item

[35] , wherein the first VWF portion and the second VWF portion are identical.

[37] The polypeptide according to item

[35] or

[36] , wherein the second subunit does not contain an erythrocyte-binding portion.

[38] The polypeptide according to any one of items

[35] to

[37] , wherein the second subunit consists essentially of a second VWF portion.

[39] The polypeptide of any one of the preceding items, wherein the erythrocyte-binding portion is capable of binding to human erythrocytes.

[40] The polypeptide of any one of the preceding items, wherein the erythrocyte-binding moiety is capable of binding to a membrane protein on an erythrocyte, preferably a human membrane protein on a human erythrocyte.

[41] The polypeptide of any one of the preceding items, wherein the erythrocyte-binding moiety is selected from the group consisting of a peptide ligand, an antibody, an antibody fragment, and a single-chain antigen-binding domain (scFv).

[42] The erythrocyte-binding moieties are band 3 (CD233), aquaporin-1, Glut-1, Kidd antigen, RhAg / Rh50 (CD241), Rh (CD240), Rh30CE (CD240CE), Rh30D (CD240D), Kx, glycophorin A (CD235a), glycophorin B (CD235b), glycophorin C (CD235c), glycophorin D (CD235d), Keil (CD238), Duffy / DARCi (CD234), CR1 (CD35), DAF (CD55), globoside, CD44, and ICAM. 11. The polypeptide of any one of the preceding claims, which is capable of specifically binding to a biological molecule selected from the group consisting of .gamma.-4 (CD242), Lu / B-CAM (CD239), XG1 / XG2 (CD99), EMMPRIN / Neurothelin (CD147), JMH, glucosyltransferase, Cartwright, Dombrock, C4A / CAB, Scimma, MER2, stomatin, BA-I (CD24), GPIV (CD36), CD108, CD139 and H antigen (CD173).

[43] A pharmaceutical composition comprising a polypeptide according to any one of the preceding items, and optionally a pharma- ceutically acceptable carrier, diluent or excipient.

[44] The pharmaceutical composition of item

[43] , wherein the dimer:monomer ratio of the polypeptide in the composition is at least 1.5, preferably at least 2, more preferably at least 2.5 or at least 3, or at least 4, or at least 5, or at least 10, or at least 20; or the composition does not contain monomeric and / or multimeric forms of the polypeptide; or the composition is essentially free of monomeric and / or multimeric forms of the polypeptide.

[45] A polypeptide according to any one of items [1] to

[42] or a pharmaceutical composition according to item

[43] or

[44] for use in treatment.

[46] A polypeptide according to any one of items [1] to

[42] or a pharmaceutical composition according to item

[43] or

[44] for use as a pharmaceutical.

[47] The polypeptide according to any one of items [1] to

[42] or the pharmaceutical composition according to item

[43] or

[44] for use in treating a blood coagulation disorder.

[48] ​​The polypeptide for use according to item

[47] , or the pharmaceutical composition for use according to item

[47] , wherein the blood coagulation disorder is hemophilia A.

[49] A polypeptide for use according to item

[47] or

[48] , or a pharmaceutical composition for use according to item

[47] or

[48] , wherein the treatment comprises administering FVIII to the subject.

[50] A polypeptide for use according to item

[49] , or a pharmaceutical composition for use according to item

[49] , wherein the polypeptide and FVIII are co-administered.

[51] A polypeptide for use according to item

[50] or a pharmaceutical composition for use according to item

[50] , wherein the co-administration is achieved (i) by administration together in a single composition comprising the polypeptide and FVIII, or (ii) by administration of the polypeptide and FVIII provided in separate compositions, whereby the polypeptide is administered before, after or simultaneously with FVIII.

[52] A polypeptide for use according to any one of items

[49] to

[51] , or a pharmaceutical composition for use according to any one of items

[49] to

[51] , wherein the ratio of the polypeptide to FVIII is at least 1, or at least 2, or at least 4, or at least 10, or at least 20, or at least 50, or at least 100.

[53] The polypeptide according to any one of items [1] to

[42] , or the pharmaceutical composition according to item

[43] or

[44] , for use in preventing or reducing inhibitor formation.

[54] The polypeptide for use according to item

[53] , or the pharmaceutical composition for use according to item

[53] , wherein the prevention or reduction of inhibitor formation comprises administering (i) the polypeptide or the pharmaceutical composition and (ii) FVIII to a subject.

[55] A nucleic acid encoding the polypeptide according to any one of items [1] to

[42] .

[56] A plasmid or vector comprising the nucleic acid according to item

[55] .

[57] A host cell containing the plasmid or vector described in item

[56] .

[58] A method for producing a polypeptide comprising a VWF and an erythrocyte-binding portion, comprising: (i) culturing a host cell of item

[57] under conditions such that the polypeptide comprising a VWF and an erythrocyte-binding portion is expressed; and (ii) optionally recovering the polypeptide comprising a VWF and an erythrocyte-binding portion from the host cell or the culture medium.

[59] A method for inducing tolerance to FVIII, comprising administering to a subject in need thereof an effective amount of a polypeptide according to any one of items [1] to

[42] .

[60] The polypeptide according to any one of items [1] to

[42] , or the pharmaceutical composition according to item

[43] or

[44] , for use in inducing tolerance to FVIII. [Brief description of the drawings]

[0009] [Figure 1] Scheme of D'D3-TER119scFv dimer. The VWF-D'D3 FVIII binding domain is fused to human albumin (HSA) and TER119scFv at the C-terminus. D'D3 is expressed alongside D1D2, the VWF propeptide (not shown). D1D2 is cleaved by co-expression of PACE / furin in the same expressing cell line. The dimer is formed via two interchain disulfides at C1099 and C1142 (illustrated by dotted lines). [Diagram 2]Formation of dimers and monomers during expression. (F) Mixture of D'D3-FP dimers and monomers derived and purified from CSL626, (T) D'D3-TER119 homodimers in CHO culture supernatant, (M) Western blot of markers. Tris Glycin 8-16%. Detection: anti-human serum albumin antibody (AP-labeled). [Diagram 3] Binding of D'D3-TER119scFv to mouse RBCs in vitro. Flow cytometry. Mouse RBCs (1:100 whole blood) were stained with anti-TER119-PE MAb and gated for single cell analysis (SSC / FSC). Mouse RBCs were then incubated with (A) PBS+1% BSA, (B) D'D3-TER119 dimer, 50 μg / ml, (C) D'D3-TER119 monomer, 50 μg / ml, (D) D'D3-TER119 monomer, 50 μg / ml, and anti-human albumin MAb, 20 μg / ml, (E) PBS+1% BSA, and (F) anti-ter119-PE mAb, 0.2 μg / ml. [Figure 4] Scheme of bispecific D'D3-TER119 heterodimer. The first VWF-D'D3 FVIII binding domain is fused to human albumin (HSA) and TER119scFv at the C-terminus. The second D'D3 is tagged at the C-terminus. D'D3 is expressed alongside D1D2, the VWF propeptide (not shown). D1D2 is cleaved by co-expression of PACE / furin in the same expressing cell line. The dimer is formed by two interchain disulfide bonds of C1099 and C1142 (illustrated by dotted lines). [Diagram 5]Expression of D'D3-TER119scFv heterodimer. Heterodimer was generated by (A) co-expression of D'D3-TER119scFv and D'D3 in a stably transfected cell line (SEQ ID NO: 3 and SEQ ID NO: 5). (B) The same expression strategy was applied with the high affinity variant of D'D3, D'D3(EYA), for both subunits (SEQ ID NO: 7 and SEQ ID NO: 9). SDS-PAGE of proteins with CaptureSelectTM Human Albumin, Ni Sepharose, Superdex-200 steps (Commassie, Tris Glycine). M: SeeBlue marker, TH: D'D3-TER119 heterodimer. F: control D'D3-FP, monomer and dimer. [Figure 6] rVIII-SingleChain binding to mouse RBCs via D'D3-TER119 heterodimers in vitro. (A) D'D3-TER119 constructs were titrated and incubated with 12.5 IU / ml human rVIII-SingleChain. Human VWF (12.5 IU / ml) was added at the indicated locations. Washed mouse RBCs (1:100 whole blood) were added to the protein mixture at 37°C. Flow cytometry. RBCs gated by SSC / FSC and TER119-PE MAb. Detection: polyclonal mouse anti-human FVIII-FITC, 20 μg / ml. D'D3WT-TER119: n=1; D'D3EYA-TER119: n=3 (independent experiments, 2 batches). (B) Exemplary fit of Michaelis-Menten kinetics to calculate the KM of D'D3EYA-TER119±VWF using batch #2. [Figure 7] Scheme of in vivo study design. Prophylactic treatment of FVIII ko mice. FVIII KO mice (n=10), 5 weekly intravenous injections. Treatment groups: rVIII-SingleChain (2000IU / kg; 160μg / kg) co-administered with D'D3EYA-TER119 heterodimer (840μg / kg, batch#1 or batch#2). [Figure 8]Prophylactic treatment of FVIII ko mice. Results of anti-FVIII-antibody production and its inhibitory effect in FVIII ko mice after administration of rVIII-SingleChain with or without D'D3EYA-TER119. Control FVIII ko mice were treated with rVIII-SingleChain alone. Test groups were treated with rVIII-SingleChain co-administered with D'D3EYA-TER119. (A) Anti-FVIII-antibody production (sum of ELISA OD extinction) and (B) its inhibitory effect (Bethesda units) in FVIII ko mice after administration of rVIII-SingleChain with or without D'D3EYA-TER119 (batch 1 or batch 2). (C) Comparison of anti-FVIII-antibody production and its inhibitory effect in FVIII ko mice after administration of rVIII-SingleChain with or without D'D3EYA-TER119. [Figure 9] Schematic representation of the therapeutic strategy to investigate long-term tolerance in FVIII ko mice. Treatment: (a) 1700 IU / kg rVIII-SingleChain and (b) co-administration with D'D3EYA-TER119 heterodimer, 672 μg / kg. Four injections, iv weekly. Interim bleed on day 28. Re-challenge with 120 IU / kg on days 49 and 56. Terminal bleed and study termination planned on day 63. [Figure 10]Graphical representation showing the effect of D'D3EYA-TER119 treatment in combination with rFVIII. Control (rVIII-SingleChain alone) and treatment group (rVIII-SingleChain+D'D3-TER119): 1700 IU / kg rVIII-SingleChain alone and co-administered with D'D3EYA-TER119 heterodimer, 672 μg / kg, at mid-day (day 28) and terminal (day 63) bleeds. Anti-FVIII antibodies were measured by (A) FVIII ADA ELISA. Interpretation was based on the predicted ratio of OD200 (sample) to OD200 (standard) corresponding to the OD at 1 / 200 dilution adapted for each titration. Statistical analysis used Kruskal-Wallis test and Dunn's multiple comparison test; *p<0.05, ***p=0.0005, ns=not significant. [Figure 11-1] FVIII levels in plasma (A) and pellet (B) in FVIII ko mice. FVIII chromogenic activity measured in pellets centrifuged and resuspended from plasma or whole blood. Three groups of mice were administered 200 IU / kg of rVIII-SingleChain alone or co-administered with D'D3EYA-FP (100 μg / kg, CSL629) or D'D3EYA-TER119 heterodimer (84 μg / kg) in equal molar ratios of FVIII to its binding partner D'D3, 1-4, and samples were taken at 5 min, 3, 8, 16, 24, 48, 72 and 96 h after administration. N=3. Data are given as mean values ​​and modeled curves. [Figure 11-2] Continued from Figure 11-1.

[0010] Detailed Description The present invention relates to a polypeptide comprising (i) a VWF portion and (ii) an erythrocyte-binding portion, said polypeptide being capable of binding to blood coagulation factor VIII (FVIII).

[0011] VWF part The term "von Willebrand factor" (VWF) as used herein includes not only naturally occurring (native) VWF but also variants thereof, such as sequence variants in which one or more residues have been inserted, deleted or substituted.

[0012] In one embodiment, the VWF is human VWF represented by the amino acid sequence set forth in SEQ ID NO: 18. The cDNA encoding SEQ ID NO: 18 is set forth in SEQ ID NO:17.

[0013] The gene encoding human native VWF is transcribed into a 9 kb mRNA, which is translated into a 2813 amino acid pre-pro polypeptide with a predicted molecular weight of 310,000 Da. The pre-pro polypeptide contains an N-terminal 22 amino acid signal peptide followed by a 741 amino acid pro-polypeptide (amino acids 23-763 of SEQ ID NO: 18) and a mature subunit (amino acids 764-2813 of SEQ ID NO: 18). Cleavage of the 741 amino acid pro-polypeptide from the N-terminus gives the mature VWF of 2050 amino acids. The amino acid sequence of the human native VWF pre-pro polypeptide is shown in SEQ ID NO: 18. Unless otherwise indicated, the amino acid numbering of VWF residues in this application refers to SEQ ID NO: 18, even if the VWF molecule, particularly the truncated VWF, does not contain all the residues of SEQ ID NO: 18.

[0014] The propolypeptide of native VWF comprises several domains. Different domain annotations can be found in the literature (e.g. Zhou et al. (2012) Blood 120(2):449-458). In the present application, the following domains of the VWF native pre-propolypeptide are defined: D1-D2-D'-D3-A1-A2-A3-D4-C1-C2-C3-C4-C5-C6-CK Apply domain annotations.

[0015] With reference to SEQ ID NO:18, the D' domain consists of amino acids 764-865; and the D3 domain consists of amino acids 866-1242.

[0016] The term "VWF portion" as used herein refers to a peptide or polypeptide having amino acid sequence similarity to human VWF as set forth in SEQ ID NO: 18, or a fragment thereof. Preferably, the sequence similarity is such that the sequence identity is at least 50%, or at least 60%, or at least 70%, or at least 80%, or at least 90%, or at least 95%, or at least 99%.

[0017] In one embodiment, the VWF portion comprises or essentially consists of a truncated VWF. The feature "truncated" in the present terminology means that the polypeptide does not contain the entire amino acid sequence of mature VWF (e.g. amino acids 764 to 2813 of SEQ ID NO: 18). According to this embodiment, the VWF portion does not contain the entire amino acids 764 to 2813 of SEQ ID NO: 18, but typically only a fragment thereof. A truncated VWF is also called a VWF fragment, or in the plural form VWF fragments.

[0018] Typically, the VWF portion can bind to factor VIII. Preferably, the VWF portion can bind to the mature form of human native factor VIII. In another embodiment, the VWF portion can bind to recombinant FVIII, such as B-domain deleted or single chain FVIII. The binding of the VWF portion to FVIII can be determined by a binding assay as described in Example 2 of WO2010 / 087271A1.

[0019] The polypeptide of the present invention can bind to FVIII.Preferably, the polypeptide of the present invention can bind to the mature form of human native factor VIII.In another embodiment, the polypeptide of the present invention can bind to recombinant FVIII, such as B-domain deleted or single chain FVIII.The binding of the polypeptide of the present invention to FVIII can be determined by binding assay as described in Example 2 of WO2010 / 087271A1.

[0020] The VWF portion of the present invention preferably comprises or consists of an amino acid sequence having at least 90% sequence identity to amino acids 776-805 of SEQ ID NO: 18 and is capable of binding to FVIII. In a preferred embodiment, the VWF portion comprises or consists of an amino acid sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to amino acids 776-805 of SEQ ID NO: 18 and is capable of binding to FVIII. In one embodiment, the VWF portion comprises or consists of amino acids 776-805 of SEQ ID NO: 18. Unless otherwise specified herein, sequence identity is determined over the entire length of the reference sequence (e.g., amino acids 776-805 of SEQ ID NO: 18).

[0021] The VWF portion of the present invention preferably comprises or consists of an amino acid sequence having at least 90% sequence identity to amino acids 766-864 of SEQ ID NO: 18 and is capable of binding to FVIII. In a preferred embodiment, the VWF portion comprises or consists of an amino acid sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to amino acids 766-864 of SEQ ID NO: 18 and is capable of binding to FVIII. In one embodiment, the VWF portion comprises or consists of amino acids 766-864 of SEQ ID NO: 18.

[0022] In another preferred embodiment, the VWF portion consists of (a) an amino acid sequence having at least 90% sequence identity to amino acids 764-1242 of SEQ ID NO: 18, or (b) a fragment thereof, provided that the VWF portion is still capable of binding to FVIII. More preferably, the VWF portion consists of (a) an amino acid sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to amino acids 764-1242 of SEQ ID NO: 18, or (b) a fragment thereof, provided that the VWF portion is still capable of binding to FVIII. In one embodiment, the VWF portion consists of (a) amino acids 764-1242 of SEQ ID NO: 18, or (b) a fragment thereof, provided that the VWF portion is still capable of binding to FVIII.

[0023] As explained in more detail below, the polypeptides of the invention can be prepared by a method using a cell containing a nucleic acid encoding a polypeptide comprising a VWF portion. The nucleic acid is introduced into a suitable host cell by techniques known per se.

[0024] In a preferred embodiment, the nucleic acid in the host cell encodes (a) an amino acid sequence having at least 90% sequence identity to amino acids 1 to 1242 of SEQ ID NO: 18, or (b) a fragment thereof, provided that the VWF portion is still capable of binding to FVIII. More preferably, the nucleic acid encodes (a) an amino acid sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to amino acids 1 to 1242 of SEQ ID NO: 18, or (b) a fragment thereof, provided that the VWF portion is still capable of binding to FVIII. In one embodiment, the nucleic acid encodes (a) amino acids 1 to 1242 of SEQ ID NO: 18, or (b) a fragment thereof, provided that the VWF portion is still capable of binding to FVIII. In particular, when the polypeptide according to the invention is a dimer, the nucleic acid will also comprise a sequence encoding amino acids 1 to 763 of VWF, even if the VWF portion in the polypeptide does not comprise amino acids 1 to 763 of VWF (e.g. SEQ ID NO: 18).

[0025] The VWF portion of the polypeptide of the invention according to a preferred embodiment may not include the amino acid sequence 1 to 763 of VWF of SEQ ID NO:18.

[0026] According to further preferred embodiments, the VWF portion comprises or consists of one of the following amino acid sequences, respectively with reference to SEQ ID NO: 18: 776-805;766-805;764-805;776-810;766-810;764-810;776-815;766-815;764-815; 776-820;766-820;764-820;776-825;766-825;764-825;776-830;766-830;764-830; 776-835;766-835;764-835;776-840;766-840;764-840;776-845;766-845;764-845; 776-850;766-850;764-850;776-855;766-855;764-855;776-860;766-860;764-860; 776-864;766-864;764-864;776-865;766-865;764-865;776-870;766-870;764-870; 776-875;766-875;764-875;776-880;766-880;764-880;776-885;766-885;764-885; 776-890;766-890;764-890;776-895;766-895;764-895;776-900;766-900;764-900; 776-905;766-905;764-905;776-910;766-910;764-910;776-915;766-915;764-915; 776-920;766-920;764-920;776-925;766-925;764-925;776-930;766-930;764-930; 776-935;766-935;764-935;776-940;766-940;764-940;776-945;766-945;764-945; 776-950;766-950;764-950;776-955;766-955;764-955;776-960;766-960;764-960; 776-965;766-965;764-965;776-970;766-970;764-970;776-975;766-975;764-975; 776-980;766-980;764-980;776-985;766-985;764-985;776-990;766-990;764-990; 776-995;766-995;764-995;776-1000;766-1000;764-1000;776-1005;766-1005;764-1005; 776-1010;766-1010;764-1010;776-1015;766-1015;764-1015;776-1020;766-1020;764-1020; 776-1025;766-1025;764-1025;776-1030;766-1030;764-1030;776-1035;766-1035;764-1035; 776-1040;766-1040;764-1040;776-1045;766-1045;764-1045;776-1050;766-1050;764-1050; 776-1055;766-1055;764-1055;776-1060;766-1060;764-1060;776-1065;766-1065;764-1065; 776-1070;766-1070;764-1070;776-1075;766-1075;764-1075;776-1080;766-1080;764-1080; 776-1085;766-1085;764-1085;776-1090;766-1090;764-1090;776-1095;766-1095;764-1095; 776-1100;766-1100;764-1100;776-1105;766-1105;764-1105;776-1110;766-1110;764-1110; 776-1115;766-1115;764-1115;776-1120;766-1120;764-1120;776-1125;766-1125;764-1125; 776-1130;766-1130;764-1130;776-1135;766-1135;764-1135;776-1140;766-1140;764-1140; 776-1145;766-1145;764-1145;776-1150;766-1150;764-1150;776-1155;766-1155;764-1155; 776-1160;766-1160;764-1160;776-1165;766-1165;764-1165;776-1170;766-1170;764-1170; 776-1175;766-1175;764-1175;776-1180;766-1180;764-1180;776-1185;766-1185;764-1185; 776-1190;766-1190;764-1190;776-1195;766-1195;764-1195;776-1200;766-1200;764-1200; 776-1205;766-1205;764-1205;776-1210;766-1210;764-1210;776-1215;766-1215;764-1215; 776-1220;766-1220;764-1220;776-1225;766-1225;764-1225;776-1230;766-1230;764-1230; 776-1235;766-1235;764-1235;776-1240;766-1240;764-1240;776-1242;766-1242;764-1242; 764-1464;764-1250;764-1041;764-828;764-865;764-1045;764-1035;764-1128;764-1198; 764-1268;764-1261;764-1264;764-1459;764-1463;764-1464;764-1683;764-1873;764-1482; 764-1479; 764-1672; and 764-1874.

[0027] In other embodiments, the VWF portion comprises or consists of an amino acid sequence having at least 90%, or at least 91%, or at least 92%, or at least 93%, or at least 94%, or at least 95%, or at least 96%, or at least 97%, or at least 98%, or at least 99% sequence identity to one of the amino acid sequences described in the previous paragraph, provided that the VWF portion is capable of binding to FVIII.

[0028] In certain embodiments, the VWF portion has an internal deletion compared to mature wild-type VWF. For example, the A1, A2, A3, D4, C1, C2, C3, C4, C5, C6, CK domains or combinations thereof may be deleted, while the D' and / or D3 domains are retained. According to further embodiments, the VWF portion lacks one or more of the domains A1, A2, A3, D4, C1, C2, C3, C4, C5, C6 or CK. According to further embodiments, the VWF portion lacks amino acids 1243 to 2813 of SEQ ID NO: 4, i.e., the domains A1-A2-A3-D4-C1-C2-C3-C4-C5-C6-CK.

[0029] In a further embodiment, the VWF moiety or polypeptide of the invention does not comprise a binding moiety for platelet glycoprotein Ibα (GPIbα), collagen and / or integrin αIIbβIII (RGDS sequence in the C1 domain). In another embodiment, the VWF moiety or polypeptide of the invention does not comprise the cleavage site of ADAMTS13 (Tyr1605-Met1606) located in the central A2 domain of VWF. In yet another embodiment, the VWF moiety or polypeptide of the invention does not comprise a binding moiety for GPIbα and / or does not comprise a binding site for collagen and / or does not comprise a binding moiety for integrin αIIbβIII and / or does not comprise the cleavage site of ADAMTS13 (Tyr1605-Met1606) located in the central A2 domain of VWF. In a preferred embodiment, the VWF moiety or polypeptide of the invention does not comprise amino acids 1691 to 1905 of SEQ ID NO: 18. In another preferred embodiment, the VWF portion or the polypeptide of the invention does not comprise amino acids 1691 to 1905 of the amino acid sequence deposited as UniProtKB-P04275. In another preferred embodiment, the VWF portion or the polypeptide of the invention does not comprise amino acids 1691 to 1905 of human VWF.

[0030] In another embodiment, the polypeptide does not comprise VWF domains A1 and A3 or a portion thereof and has low or essentially no affinity for collagen types I and III, said low or essentially no affinity being less than or equal to a dissociation constant K for binding of the polypeptide to collagen types I and III. D Characterized by >10μM.

[0031] A polypeptide of the present invention is referred to as a "dimer" in the present invention when two monomers of the polypeptide of the present invention are covalently linked. Preferably, the covalent link is located in the VWF portion of the polypeptide of the present invention. Preferably, the two monomer subunits are covalently linked via at least one disulfide bridge, for example by 1, 2, 3 or 4 disulfide bridges. The cysteine ​​residues forming at least one disulfide bridge are preferably located in the VWF portion of the polypeptide of the present invention. In one embodiment, these cysteine ​​residues are Cys-1099, Cys-1142, Cys-1222, Cys-1225 or Cys-1227, or a combination thereof. Preferably, the dimeric polypeptide of the present invention does not contain any further covalent bonds linking the monomers in addition to the above-mentioned covalent bonds located in the VWF portion of the polypeptide, in particular does not contain any further covalent bonds located in the HLEM or HLEP portion of the polypeptide. However, according to alternative embodiments, the dimeric polypeptides of the invention may comprise a covalent bond located within the HLEM or HLEP portion of the polypeptide that links the monomers.

[0032] The dimer is preferably a heterodimer. When the polypeptide of the present invention is a dimer, each monomer preferably independently comprises an amino acid sequence having at least 90% sequence identity to amino acids 764-1099, 764-1142, 764-1222, 764-1225, 764-1227 or 764-1242 of SEQ ID NO: 18, and is capable of binding to FVIII. In a preferred embodiment, the VWF portion in each subunit independently comprises or essentially consists of an amino acid sequence having at least 95%, at least 96%, at least 97%, at least 98% or at least 99% sequence identity to amino acids 764-1099, 764-1142, 764-1222, 764-1225, 764-1227 or 764-1242 of SEQ ID NO: 18, and is capable of binding to FVIII. In one embodiment, the VWF portion of each monomer comprises or consists essentially of amino acids 764 to 1099, amino acids 764 to 1142, amino acids 764 to 1222, amino acids 764 to 1225, amino acids 764 to 1227 or amino acids 764 to 1242 of SEQ ID NO:18.

[0033] The VWF portion may be or may consist essentially of any one of the VWF fragments disclosed in WO2013 / 106787A1, WO2014 / 198699A2, WO2011 / 060242A2 or WO2013 / 093760A2, the disclosures of which are incorporated herein by reference.

[0034] According to a further preferred embodiment, the VWF portion as disclosed above may comprise at least one of the amino acid substitutions as disclosed in WO2016 / 000039A1 or WO2017 / 117631A1. These modified versions of the VWF portion comprise at least one amino acid substitution in its D' domain compared to the amino acid sequence of the D' domain of wild type VWF according to SEQ ID NO: 18. The amino acid sequence of the modified version of the VWF portion can have one or more amino acid substitutions compared to the respective wild type sequence. The amino acid sequence of the D' domain of the modified VWF portion preferably has one or two or three amino acid substitutions with respect to the D' domain of SEQ ID NO: 18. It is preferred that the S at position 764 of SEQ ID NO: 18, which corresponds to position 1 of SEQ ID NO: 20, is replaced with an amino acid selected from the group consisting of G, P, V, E, Y, A and L. It is also preferred that the S at position 766 of SEQ ID NO: 18, which corresponds to position 3 of SEQ ID NO: 20, is replaced with an amino acid selected from the group consisting of Y, I, M, V, F, H, R and W. It is further preferred that the V at position 1083 of SEQ ID NO: 18 is substituted with the amino acid alanine (A). Preferred substitution combinations include, with reference to the sequence of SEQ ID NO:18, S764G / S766Y, S764P / S766I, S764P / S766M, S764V / S766Y, S764E / S766Y, S764Y / S766Y, S764L / S766Y, S764P / S766W, S766W / 806A, S766Y / P769K, S766Y / P769N, S766Y / P769R, S764P / S766L, S764G / S766Y / V1083A(GYA), S764E / S766Y / V1083A(EYA) and S766Y / V1083A(YA). Most preferred is the combination of substitutions S764E / S766Y / V1083A (EYA), with reference to sequence SEQ ID NO:18.

[0035] The binding affinity of the polypeptide of the present invention to FVIII can be further increased by the introduction of the above substitutions compared to the binding affinity of a reference polypeptide having the same amino acid sequence except for the above modification. The above substitutions in the VWF moiety can contribute to increasing the half-life of co-administered FVIII or the stability of co-administered FVIII.

[0036] Red blood cell binding part The terms "erythrocyte," "red blood cell," and "RBC" have the same meaning and are used interchangeably herein.

[0037] The erythrocyte binding moiety is preferably a peptide or polypeptide capable of binding to a molecule exposed on the surface of an erythrocyte, preferably a human erythrocyte. Preferably, the molecule exposed on the surface of an erythrocyte is a membrane protein on the surface of an erythrocyte, preferably a human erythrocyte. More preferably, the molecule exposed on the surface of an erythrocyte, preferably a human erythrocyte, is selected from the group consisting of Band3 (CD233), aquaporin-1, Glut-1, Kidd antigen, RhAg / Rh50 (CD241), Rh (CD240), Rh30CE (CD240CE), Rh30D (CD240D), Kx, glycophorin A (CD235a), glycophorin B (CD235b), glycophorin C (CD235c), glycophorin D (CD235d), Keil (CD238), Duffy / DARCi (CD234), CR1 (CD35). , DAF (CD55), globoside, CD44, ICAM-4 (CD242), Lu / B-CAM (CD239), XG1 / XG2 (CD99), EMMPRIN / Neurothelin (CD147), JMH, glycosyltransferase, Cartwright, Dombrock, C4A / CAB, Scimma, MER2, stomatin, BA-I (CD24), GPIV (CD36), CD108, CD139 and H antigen (CD173).

[0038] Preferably, the erythrocyte-binding moiety is a peptide or polypeptide capable of specifically binding to a molecule selected from the group consisting of glycophorin A (CD235a), glycophorin B (CD235b), glycophorin C (CD235c), and glycophorin D (CD235d). Most preferably, the erythrocyte-binding moiety is capable of specifically binding to glycophorin A (CD235a).

[0039] Suitable erythrocyte-binding moieties are described in WO2019 / 075523A1, WO2014 / 135528A1, WO2018 / 093766A1, WO2013 / 121296A1 and WO2012 / 021512A2, the disclosures of which are incorporated herein in their entirety. Examples of erythrocyte-binding moieties that bind to human erythrocytes include polypeptides comprising or consisting essentially of SEQ ID NO:21 or SEQ ID NO:23. SEQ ID NO:21 shows an exemplary amino acid sequence of a VHH nanobody designated IH4 as disclosed in WO2014 / 135528A1. SEQ ID NO:23 shows the amino acid sequence of the hybridoma G26.4.1C3 / 86 [RAT 1C3 / 86] (ATCC 612246). (R) HB-9893 TM ) scFv 1C3 obtained from

[0040] Suitable erythrocyte-binding moieties can be provided by phage display (e.g., as described in WO2018 / 093766A1) and hybridoma techniques.

[0041] Preferably, the polypeptide of the invention is a fusion protein in which the VWF portion and the erythrocyte-binding portion are fused, optionally via a linker sequence.

[0042] Half-life extension moiety (HLEM) In addition to the VWF portion and the erythrocyte binding portion, the polypeptide of the present invention may further comprise a half-life extending portion in a preferred embodiment. The half-life extending portion may be a heterologous amino acid sequence fused to the VWF portion. Alternatively, the half-life extending portion may be chemically conjugated to the polypeptide comprising the VWF portion by a covalent bond other than a peptide bond. Preferably, the half-life extending portion does not induce dimerization or multimerization. Preferably, the half-life extending portion cannot form dimers or multimers.

[0043] In certain embodiments of the present invention, the half-life of the polypeptide of the present invention is extended by chemical modification, for example, by adding a half-life extending moiety such as polyethylene glycol (PEGylation), glycosylated PEG, hydroxyethyl starch (HES), polysialic acid, elastin-like polypeptide, heparosan polymer or hyaluronic acid.In another embodiment, the polypeptide of the present invention is conjugated to HLEM such as albumin via a chemical linker.The principle of this conjugation technology is exemplarily described by Conjuchem LLC (see, for example, U.S. Patent No. 7,256,253).

[0044] In another embodiment, the half-life extending moiety is a half-life enhancing polypeptide (HLEP). Preferably, the HLEP is albumin or a fragment thereof. The N-terminus of the albumin may be fused to the C-terminus of the VWF moiety. Alternatively, the C-terminus of the albumin may be fused to the N-terminus of the VWF moiety. One or more HLEPs may be fused to the N-terminus or C-terminus of the VWF moiety, provided that they do not interfere with or abolish the binding ability of the VWF moiety to FVIII.

[0045] The recombinant polypeptide preferably further comprises a covalent bond located between the VWF moiety and the HLEM or a linker sequence located between the VWF moiety and the HLEP.

[0046] The linker sequence may be a peptide linker consisting of one or more amino acids, in particular 1-50, 1-30, 1-20, 1-15, 1-10, 1-5 or 1-3 (e.g. 1, 2 or 3) amino acids, which may be identical or different from each other. Preferably, the linker sequence is not present at the corresponding position in wild-type VWF. Preferred amino acids present in the linker sequence include Gly and Ser. The linker sequence should be non-immunogenic. A preferred linker may be composed of alternating glycine and serine residues. Suitable linkers are described, for example, in WO2007 / 090584A1.

[0047] In another embodiment of the present invention, the peptide linker between the VWF portion and the HLEP is composed of a peptide sequence that functions as a natural interdomain linker or sequence in human proteins.Preferably, such a peptide sequence is located close to the protein surface and accessible to the immune system in its natural environment, so that it can carry out self-tolerance to this sequence.Examples are given in WO2007 / 090584A1.Cleaving linker sequences are described, for example, in WO2013 / 120939A1.

[0048] In a preferred embodiment of the recombinant polypeptide, the linker between the VWF portion and the HLEP is a glycine / serine peptide linker having or consisting of amino acid sequence 480-510 of SEQ ID NO:2.

[0049] In one embodiment, the polypeptide has the structure: VWFM-L1-H-L2-EBM, [Equation 1] wherein VWFM is the VWF moiety, L1 is a chemical bond or a linker sequence, H is a HLEM, in particular a HLEP, L2 is a chemical bond or a linker sequence, and EBM is the erythrocyte-binding moiety.

[0050] L1 and L2 may independently be a chemical bond or a linker sequence consisting of one or more amino acids, for example 1-50, 1-30, 1-20, 1-15, 1-10, 1-5 or 1-3 (e.g. 1, 2 or 3) amino acids, which may be the same or different from each other. Typically, the linker sequence is not present at the corresponding position in wild-type VWF. Examples of suitable amino acids present in L1 and / or L2 include Gly and Ser. The linker must be non-immunogenic and may be a non-cleavable or cleavable linker. A non-cleavable linker may be composed of alternating glycine and serine residues, as exemplified in WO2007 / 090584A1. In another embodiment of the invention, the peptide linker between the VWF moiety and the albumin moiety consists of a peptide sequence that functions as a natural interdomain linker or sequence in human proteins. Preferably, such peptide sequences are located close to the protein surface and accessible to the immune system in its natural environment, so that self-tolerance to this sequence can be mediated. Examples are given in WO2007 / 090584A1. Cleavable linker sequences are described, for example, in WO2013 / 120939A1.

[0051] Preferred HLEP sequences are described below. Also encompassed by the present invention are fusions to the exact "N-terminal amino acid" or the exact "C-terminal amino acid" of the respective HLEP, or to the "N-terminal part" or "C-terminal part" of the respective HLEP, including N-terminal deletion of one or more amino acids of the HLEP. A polypeptide may contain more than one HLEP sequence, for example two or three HLEP sequences. These multiple HLEP sequences may be fused in tandem, for example as consecutive repeats, to the C-terminal part of VWF.

[0052] Half-life enhancing polypeptide (HLEP) Preferably, the half-life extending moiety is a half-life enhancing polypeptide (HLEP). More preferably, the HLEP is albumin, a member of the albumin-family or a fragment thereof, a solvated random chain with a large hydrodynamic volume (e.g., XTEN (Schellenberger et al., 2009; Nature 103:131-135, 2011) or a fragment thereof. Biotechnol. 27:1186-1190), homoamino acid repeats (HAP) or proline-alanine-serine repeats (PAS), afamin, alpha-fetoprotein, vitamin D binding protein, transferrin or a variant or fragment thereof, the carboxyl terminal peptide (CTP) of the human chorionic gonadotropin-β subunit, a polypeptide capable of binding to the neonatal Fc receptor (FcRn), in particular an immunoglobulin constant region and a part thereof, such as an Fc fragment, a polypeptide or lipid capable of binding to albumin, to a member of the albumin family or a fragment thereof, or to an immunoglobulin constant region or a part thereof under physiological conditions. The immunoglobulin constant region or a part thereof is preferably an Fc fragment of immunoglobulin G1, an Fc fragment of immunoglobulin G2 or an Fc fragment of immunoglobulin A.

[0053] Preferably, the HLEP does not induce dimerization or multimerization. Preferably, the HLEP is incapable of forming dimers or multimers.

[0054] As used herein, a half-life enhancing polypeptide may be a full-length half-life enhancing protein as described herein, or one or more fragments thereof that are capable of stabilizing or extending the therapeutic or biological activity of the coagulation factor, in particular increasing the in vivo half-life of the polypeptide of the invention. Such fragments may be 10 or more amino acids in length, or may comprise at least about 15, at least about 20, at least about 25, at least about 30, at least about 50, at least about 100, or more contiguous amino acids from the HLEP sequence, or may comprise some or all of a particular domain of the respective HLEP, so long as the HLEP fragment provides at least a 25% functional half-life extension compared to the respective polypeptide without the HLEP.

[0055] The HLEP portion of the polypeptide of the invention may be a variant of the wild-type HLEP. The term "variant" includes insertions, deletions and substitutions, either conservative or non-conservative, which changes do not substantially alter the FVIII binding activity of the VWF portion.

[0056] In particular, the proposed VWF moiety-HLEP fusion construct of the present invention may include naturally occurring polymorphic variants of HLEP and fragments of HLEP. The HLEP may be derived from any vertebrate, particularly any mammal, such as human, monkey, cow, sheep, or pig. Non-mammalian HLEP includes, but is not limited to, hen and salmon.

[0057] According to certain embodiments of the present disclosure, the HLEM, and in particular the HLEP, portion of the recombinant polypeptide of the present invention may be identified by the alternative term "FP." Preferably, the term "FP" stands for human albumin.

[0058] According to a particular preferred embodiment, the recombinant polypeptide is a fusion protein. A fusion protein in the context of the present invention is a protein created by in-frame ligation of at least two DNA sequences encoding a VWF moiety and an HLEP. Those skilled in the art will understand that translation of a fusion protein DNA sequence results in a single protein sequence. As a result of in-frame insertion of a DNA sequence encoding a peptide linker according to a further preferred embodiment, a fusion protein comprising a VWF moiety, a suitable linker and an HLEP can be obtained.

[0059] According to some embodiments, the co-formulated FVIII does not include any of the HLEM or HLEP structures described herein. According to certain other embodiments, the co-formulated FVIII may include at least one of the HLEM or HLEP structures described herein.

[0060] Albumin as a HLEP The terms "human serum albumin" (HSA) and "human albumin" (HA) are used interchangeably in this application. The terms "albumin" and "serum albumin" are broader and encompass human serum albumin (and fragments and variants thereof), and albumins (and fragments and variants thereof) from other species.

[0061] As used herein, "albumin" refers collectively to an albumin polypeptide or amino acid sequence, or an albumin fragment or variant having one or more functional properties (e.g., biological functions) of albumin. In particular, "albumin" refers to human albumin or a fragment thereof, particularly the mature form of human albumin as set forth herein in SEQ ID NO: 19, or albumin from other vertebrates or a fragment thereof, or an analog or variant of these molecules or fragments thereof.

[0062] According to certain embodiments of the present disclosure, the alternative term "FP" is used to identify HLEPs, and in particular to define albumin as an HLEP.

[0063] In particular, the proposed polypeptides of the present invention may include naturally occurring polymorphic variants of human albumin and fragments of human albumin. Generally, albumin fragments or variants will be at least 10, preferably at least 40, and most preferably 70 or more amino acids in length.

[0064] A preferred embodiment of the present invention comprises albumin variants used as HLEPs of the polypeptides of the present invention that have enhanced binding to the FcRn receptor, which may induce a longer plasma half-life of the VWF partial albumin variant fusion protein compared to the fusion of the VWF portion with wild-type albumin.

[0065] The albumin portion of a polypeptide of the invention may comprise at least one subdomain or domain of HA, or a conservative modification thereof.

[0066] Immunoglobulins as HLEPs The immunoglobulin G (IgG) constant region (Fc) is known in the art to increase the half-life of therapeutic proteins (Dumont JA et al., 2006. BioDrugs 20:151-160). The IgG constant region of the heavy chain consists of three domains (CH1-CH3) and a hinge region. The immunoglobulin sequence may be from any mammal or subclass IgG1, IgG2, IgG3 or IgG4, respectively. IgG and IgG fragments without antigen binding domains may also be used as HLEPs. The therapeutic polypeptide moiety is preferably linked to the IgG or IgG fragment via the hinge region of the antibody or a peptide linker, which may be cleavable. Several patents and patent applications describe fusing therapeutic proteins to immunoglobulin constant regions to enhance the in vivo half-life of the therapeutic protein. US2004 / 0087778 and WO2005 / 001025A2 describe fusion proteins of at least a portion of an Fc domain or immunoglobulin constant region with a biologically active peptide that increases the half-life of the peptide, but is otherwise rapidly cleared in the body. Fc-IFN-β fusion proteins are described that achieve enhanced biological activity, extended circulating half-life, and increased solubility (WO2006 / 000448A2). Fc-EPO proteins with extended serum half-lives and increased potency in vivo (WO 2005 / 063808 A1) as well as Fc fusions with G-CSF (WO 2003 / 076567 A2), glucagon-like peptide-1 (WO 2005 / 000892 A2), clotting factors (WO 2004 / 101740 A2) and interleukin 10 (U.S. Patent No. 6,403,077) have all been disclosed to have enhanced half-lives.

[0067] Preferably, the immunoglobulin or Fc portion used as the HLEP does not induce dimerization or multimerization. Preferably, the immunoglobulin or Fc portion used as the HLEP is incapable of forming dimers or multimers.

[0068] Various HLEPs that can be used in accordance with the present invention are described in detail in WO2013 / 120939A1.

[0069] dimer The polypeptide of the present invention may have a high ratio of dimers.Therefore, the polypeptide of the present invention is preferably present as a dimer.In one embodiment, at least 50%, or at least 60%, or at least 70%, or at least 80%, or at least 90%, or at least 95%, or at least 98% of the polypeptide is present as a dimer.Most preferably, essentially all the polypeptide of the present invention is present as a dimer.The dimer is preferably a heterodimer.

[0070] It is further preferred that the polypeptide of the present invention does not contain multimers. The use of dimers is preferred since dimers have improved affinity for factor VIII compared to monomers. The dimer content and the ratio of dimer to monomer of the polypeptide of the present invention can be determined by size exclusion chromatography or HPLC, for example as described in WO2010 / 087271A1, page 56, lines 6-10. Alternatively, the dimer content and the ratio of dimer to monomer can be determined by SDS-PAGE and Western blot, see the examples of this application.

[0071] Unless otherwise specified, all molar concentrations of a polypeptide of the invention described herein refer to the molar concentration of a dimer of the polypeptide of the invention, regardless of whether it actually exists as a homodimer or heterodimer.

[0072] In one embodiment, the affinity of the polypeptide of the invention for factor VIII is greater than that of human native VWF for the same factor VIII molecule. The factor VIII affinity of the polypeptide may refer to either human native, plasma-derived or recombinant factor VIII, particularly recombinant factor VIII molecules with truncated or deleted B domains.

[0073] It has been found that preparations of the polypeptides of the invention with a high dimer ratio have an increased affinity for factor VIII.Also, instead of or in combination with an increased dimer ratio, the polypeptides according to the invention with mutations in the factor VIII binding domain are also preferred embodiments of the invention that increase the affinity for factor VIII.Suitable mutations are disclosed, for example, in WO2013 / 120939A1.

[0074] Preferably, the polypeptide of the present invention is a heterodimer. In one embodiment, the heterodimer comprises a first subunit and a second subunit, the first subunit comprising a first VWF moiety and an erythrocyte binding moiety as defined herein, the second subunit comprising a second VWF moiety as defined herein, and the second subunit not comprising an erythrocyte binding moiety. The VWF moieties in the first and second subunits (monomers) are preferably identical.

[0075] In one embodiment, the second subunit consists essentially of a second VWF portion.

[0076] In another embodiment, the two monomers forming the dimer are covalently linked to each other via at least one disulfide bridge formed by cysteine ​​residues in the VWF moiety. The cysteine ​​residues forming one or more disulfide bridges may be selected from the group consisting of Cys-1099, Cys-1142, Cys-1225, Cys-1227 and combinations thereof, preferably Cys-1099 and Cys-1142, with amino acid numbering see SEQ ID NO: 18.

[0077] Preparation of Polypeptides Nucleic acids encoding the polypeptides of the present invention can be prepared according to methods known in the art. Based on the cDNA sequence of the pre-pro form of human native VWF (SEQ ID NO: 17), recombinant DNA encoding the above VWF partial constructs or the polypeptides of the present invention can be designed and produced.

[0078] Even if the polypeptide secreted from a host cell does not contain amino acids 1 to 763 of the pre-pro form of human VWF, it is preferred that the nucleic acid (e.g., DNA) encoding the intracellular precursor of the polypeptide comprises a nucleic acid sequence encoding amino acids 23 to 763 of SEQ ID NO: 18, or preferably an amino acid sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to amino acids 1 to 763 of SEQ ID NO: 18. Most preferably, the nucleic acid (e.g., DNA) encoding the intracellular precursor of the polypeptide comprises a nucleotide sequence encoding amino acids 23 to 763 of SEQ ID NO: 18, or amino acids 1 to 763 of SEQ ID NO: 18.

[0079] Constructs containing the entire open reading frame, with the DNA inserted in the correct orientation into an expression plasmid, can be used to express proteins. A typical expression vector contains a promoter that directs the synthesis of large amounts of mRNA corresponding to the inserted nucleic acid in cells harboring the plasmid. It may also contain an origin of replication sequence that allows its autonomous replication in the host organism, and sequences that increase the efficiency with which the synthesized mRNA is translated. Stable long-term vectors can be maintained as freely replicating entities, for example, by using viral control elements (e.g., the OriP sequence of the Epstein Barr Virus genome). Cell lines may also be produced that incorporate the vector into their genomic DNA, in which case the gene product is produced continuously.

[0080] Typically, the provided cells are obtained by introducing a nucleic acid encoding a polypeptide of the invention into a mammalian host cell.

[0081] Any host cell susceptible to cell culture and expression of glycoproteins can be utilized in accordance with the present invention, hi certain embodiments, the host cell is mammalian. Non-limiting examples of mammalian cells that may be used in accordance with the present invention include BALB / c mouse myeloma line (NSO / 1, ECACC number: 85110503); human retinoblastoma cells (PER.C6 (CruCell, Leiden, The Netherlands)); monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, Graham et al., J. Gen Virol., 36:59, 1977); baby hamster kidney cells (BHK, ATCC CCL10); Chinese hamster ovary cells + / - DHFR (CHO, Urlaub and Chasin, Proc. Natl. Acad. Sci. USA, 77:4216, 1980); mouse Sertoli cells (TM4, Mather, Biol. Reprod, 23:243 251, 1980); monkey kidney cells (CV1 ATCC CCL 70); African green monkey kidney cells (VERO-76, ATCC CRL-1 587); human cervical carcinoma cells (HeLa, ATCC CCL 2); canine kidney cells (MDCK, ATCC CCL 34); buffalo rat hepatocytes (BRL 3A, ATCC CRL 1442); human lung cells (W138, ATCC CCL 75); human hepatocytes (HepG2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI cells (Mather et al., Annals NY. Acad. Sci., 383:44-68, 1982); MRC5 cells; PS4 cells; human amniotic cells (CAP); and human hepatocellular carcinoma cell line (Hep G2). Preferably, the cell line is a rodent cell line, particularly a hamster cell line such as CHO or BHK, or a human cell line.

[0082] Suitable methods for introducing sufficient nucleic acid into mammalian host cells to achieve expression of a glycoprotein of interest are known in the art. See, for example, Getting et al., Nature, 293:620-625, 1981; Mantei et al., Nature, 281:40-46, 1979; Levinson et al., EP0117060; and EP0117058. For mammalian cells, common methods for introducing genetic material into mammalian cells include the calcium phosphate precipitation method of Graham and van der Erb (Virology, 52:456-457, 1978) or the lipofectamine method of Hawley-Nelson. TM (Gibco BRL) Method (Focus 15:73, 1993). General aspects of mammalian cell host system transformation are described by Axel in U.S. Patent No. 4,399,216. For various techniques for introducing genetic material into mammalian cells, see Keown et al., Methods in Enzymology, 185:527-537, 1990, and Mansour et al., Nature, 336:348-352, 1988.

[0083] The cells are cultured under conditions that allow expression of the polypeptide, which can be recovered and purified using methods known to those of skill in the art.

[0084] therapeutic use The polypeptides of the present invention are useful for the treatment of clotting disorders, including hemophilia A. The term "hemophilia A" refers to a deficiency in functional clotting FVIII, which is usually inherited.

[0085] A further aspect of the present invention is a method for treating a blood clotting disorder, comprising administering to a patient in need thereof an effective amount of a polypeptide as defined herein above.

[0086] Treatment of a disease includes treatment of a patient already diagnosed with any form of the disease at any clinical stage or symptom; delaying the onset or progression or exacerbation or worsening of symptoms or signs of the disease; and preventing and / or reducing the severity of the disease.

[0087] A "subject" or "patient" to whom the polypeptides of the invention are administered is preferably a human. In certain embodiments, the human is a pediatric patient. In other embodiments, the human is an adult patient.

[0088] Compositions comprising the polypeptides of the present invention are described herein.The compositions are typically provided as part of a sterile pharmaceutical composition that includes a pharma- ceutically acceptable carrier.The compositions can take any suitable form (depending on the desired method of administering it to a patient).

[0089] The polypeptide of the present invention can be administered to a patient by various extravascular routes, such as subcutaneous, intradermal or intramuscular. The most appropriate administration route in any given case will depend on the particular polypeptide, the subject, and the nature and severity of the disease and physical condition of the subject. Preferably, the polypeptide of the present invention will be administered subcutaneously.

[0090] In one embodiment, the treatment comprises administering the polypeptide of the present invention as the only active ingredient. In another embodiment, the treatment comprises administering the polypeptide of the present invention in combination with at least one additional active ingredient. The polypeptide of the present invention and at least one additional active ingredient can be administered simultaneously, separately or sequentially.

[0091] Preferably, the further active ingredient is FVIII. Thus, the method of the present invention preferably comprises administering to a patient an effective amount of FVIII. The polypeptide of the present invention and FVIII are preferably co-administered subcutaneously.

[0092] The determination of the total number of administrations and the length of treatment with the polypeptide of the present invention and FVIII is well within the capabilities of a person skilled in the art. The dosage of the polypeptide of the present invention and the administered FVIII depend on the concentration of the administered FVIII. The degree of severity of the blood coagulation disorder may also be considered to determine the appropriate dosage of the polypeptide of the present invention and the administered FVIII. A typical dosage of FVIII may range from about 20 IU / kg body weight to about 1000 IU / kg body weight, preferably from about 20 IU / kg body weight to about 500 IU / kg body weight, more preferably from about 20 IU / kg body weight to about 400 IU / kg body weight, and more preferably from about 20 IU / kg body weight to about 300 IU / kg body weight.

[0093] According to the present invention, the patient treated with the polypeptide of the present invention is also treated with blood coagulation factor VIII. The polypeptide of the present invention and factor VIII can be administered preferably simultaneously, i.e. together, but administration in a sequential manner can also be carried out in principle, both modes of administration being encompassed by the terms "combination therapy" and "co-administration". The polypeptide of the present invention and factor VIII can be administered as a mixture, i.e. in the same composition, or separately, i.e. as separate compositions. Co-administration of the recombinant polypeptide and the FVIII protein is preferably achieved by administering them together in a single composition comprising the recombinant polypeptide and the FVIII protein. According to a further preferred embodiment, co-administration of the recombinant polypeptide and the FVIII protein is achieved by providing a combined product comprising the recombinant polypeptide and FVIII formulated in a single composition, or by providing a set or kit of at least two separate products prepared to be mixed before administration, whereby the first product comprises the recombinant polypeptide and the second product comprises FVIII. In particular, when recombinant polypeptide and FVIII protein are provided in separate compositions or products that are mixed before co-administration, the mixture can be treated before administration in such a way that at least a proportion of the recombinant polypeptide can bind to the FVIII before administration.For example, the mixture can be incubated for a certain period of time.Such incubation can be carried out at either ambient temperature or at an elevated temperature if necessary, but preferably at a temperature of less than 40°C, for less than 1 minute, or less than 5 minutes.Such a rapid incubation step can also be suitable during reconstitution for a combined product that contains recombinant polypeptide and FVIII combined in a single composition.

[0094] The concentration of factor VIII in the composition used is typically in the range of 10 to 10,000 IU / mL. In different embodiments, the concentration of FVIII in the composition of the invention is 10 to 8,000 IU / mL, or 10 to 5,000 IU / mL, or 20 to 3,000 IU / mL, or 50 to 1,500 IU / mL, or 3,000 IU / mL, or 2,500 IU / mL, or 2,000 IU / mL, or 1,500 IU / mL, or 1,200 IU / mL, or 1,000 IU / mL, or 800 IU / mL, or is in the range of 750 IU / mL, or 600 IU / mL, or 500 IU / mL, or 400 IU / mL, or 300 IU / mL, or 250 IU / mL, or 200 IU / mL, or 150 IU / mL, or 125 IU / mL, or 100 IU / mL, or 62.5 IU / mL, or 50 IU / mL, provided that the ratio requirements for the VWF polypeptide of the present invention defined herein are met.

[0095] "International Unit" or "IU" is a unit of measurement of blood clotting activity (potency) of FVIII measured by FVIII activity assays such as one-stage clotting assays or chromogenic substrate FVIII activity assays using standards calibrated in "IU" against international reference specimens. One-stage clotting assays are known to those skilled in the art, for example as described in N Lee, Martin L et al., An Effect of Predilution on Potency Assays of FVIII Concentrates, Thrombosis Research (Pergamon Press Ltd.) 30, 511 519 (1983). Principle of the one-stage assay: The test is performed as a modified version of the activated partial thromboplastin time (aPTT) assay: activation of factors of the intrinsic clotting system is induced by incubating plasma with phospholipids and a surface activator. The addition of calcium ions initiates the clotting cascade. The time until a measurable fibrin clot is formed is measured. The assay is performed in the presence of factor VIII-deficient plasma. The clotting ability of factor VIII-deficient plasma is restored by factor VIII present in the sample being tested. The shortening of the clotting time is proportional to the amount of factor VIII present in the sample. Factor VIII activity is quantified by direct comparison with a standard preparation of known factor VIII activity in international units. Another standard assay is the chromogenic substrate assay. Chromogenic assays are known as Coamatic (R) It may be purchased commercially, such as the FVIII test kit (Chromogenix-Instrumentation Laboratory SpA V.le Monza 338-20128 Milan, Italy). Chromogenic assay principle: In the presence of calcium and phospholipids, factor X is activated from factor IXa to factor Xa. This reaction is stimulated by factor VIIIa as a cofactor. FVIIIa is generated from FVIII in the sample to be measured by a small amount of thrombin in the reaction mixture.

[0096] Optimal concentration of Ca2 +When using phospholipids and factor IXa, as well as an excess of factor X, activation of factor X is proportional to the potency of factor VIII. Activated factor X releases the chromophore pNA from the chromogenic substrate S-2765. The amount of pNA released, measured at 405 nm, is therefore proportional to the amount of FXa formed and therefore to the factor VIII activity of the sample.

[0097] When the polypeptide of the present invention and FVIII are used in combination therapy, the ratio of the polypeptide to FVIII administered can be any ratio as defined in the "ratio" section below.In another aspect, the present invention relates to the polypeptide of the present invention for use in increasing the half-life of FVIII in vivo.Yet another aspect of the present invention is a method for increasing the half-life of FVIII in a subject, comprising administering to the subject an effective amount of the polypeptide of the present invention or the pharmaceutical composition of the present invention.The polypeptide of the present invention and FVIII can be co-administered to the subject simultaneously, separately or sequentially.

[0098] In yet another aspect, the present invention relates to a polypeptide of the invention for use in preventing and / or reducing inhibitor formation in vivo. Yet another aspect of the present invention is a method for reducing and / or preventing inhibitor formation in a subject treated with FVIII, said method comprising administering to the subject an effective amount of a polypeptide of the invention.

[0099] Factor VIII As used herein, the term "factor VIII" or "FVIII" refers to a molecule that has at least a portion of the clotting activity of human native factor VIII. Human FVIII consists of 2351 amino acids (including the signal peptide) and 2332 amino acids (excluding the signal peptide). "Human native FVIII" is a human plasma-derived FVIII molecule having the full-length sequence (amino acids 1-2332) as shown in SEQ ID NO:20. The detailed domain structure, A1-a1-A2-a2-B-a3-A3-C1-C2, has the corresponding amino acid residues (see SEQ ID NO:20): A1(1-336), a1(337-372), A2(373-710), a2(711-740), B(741-1648), a3(1649-1689), A3(1690-2020), C1(2021-2173) and C2(2174-2332). The FVIII referred to herein may be plasma-derived FVIII (pdFVIII) or recombinantly produced FVIII (recombinant FVIII).

[0100] The coagulation activity of the FVIII molecule can be determined using a one-stage coagulation assay (e.g., as described by Lee et al., Thrombosis Research 30, 511 519 (1983)) or a chromogenic substrate assay (e.g., the coamatic FVIII test kit, Chromogenix-Instrumentation Laboratory SpA V.le Monza 338-20128 Milan, Italy). Further details of these activity assays are described below.

[0101] Preferably, the FVIII molecule used according to the invention has at least 10% of the specific molar activity of human native FVIII. The term "specific molar activity" refers to the clotting activity per mole of FVIII, e.g. expressed in "IU / mole" FVIII or - more conveniently - "IU / picomole" FVIII.

[0102] In a preferred embodiment, the FVIII molecule is a non-naturally occurring FVIII molecule. Preferably, the non-naturally occurring FVIII molecule is recombinantly produced. In another embodiment, the FVIII molecule is produced in cell culture. In another preferred embodiment, the non-naturally occurring FVIII molecule has a glycosylation pattern different from that of plasma-derived FVIII. In yet another embodiment, the FVIII molecule is selected from the group consisting of: (i) a B-domain deleted or truncated FVIII molecule, (ii) a single-chain FVIII molecule, (iii) a recombinantly produced two-chain FVIII molecule, (iv) a FVIII molecule with a protecting group or a half-life extension moiety, (v) a fusion protein comprising a FVIII amino acid sequence fused to a heterologous amino acid sequence, and (vi) a combination thereof.

[0103] The terms "Factor VIII" and "FVIII" are used interchangeably herein. A "Factor VIII composition" in the sense of the present invention includes a composition comprising FVIII and FVIIIa. FVIIIa may typically be present in small amounts, e.g., about 1-2% FVIIIa, based on the total amount of FVIII protein in the composition. Proteolytically cleaved FVIII may typically be present in small to medium amounts, e.g., about 1-50%, based on the total amount of FVIII protein in the composition. "FVIII" includes natural allelic variants of FVIII that may exist and occur from one individual to another. FVIII may be plasma-derived or recombinantly produced, using well-known production and purification methods. The extent and location of glycosylation, tyrosine sulfation and other post-translational modifications may vary depending on the host cell selected and its growth conditions.

[0104] The term FVIII includes FVIII analogs. As used herein, the term "FVIII analog" refers to a FVIII molecule (full length or B domain truncated / deleted) in which one or more amino acids have been substituted or deleted compared to SEQ ID NO: 20, or the corresponding portion of SEQ ID NO: 20 for a B domain truncated / deleted FVII molecule. FVIII analogs do not occur in nature, but are obtained by artificial manipulation.

[0105] The factor VIII molecule used according to the invention may also be a B-domain truncated / deleted FVIII molecule, the remaining domains of which correspond to the sequence as set forth in amino acid numbers 1 to 740 and 1649 to 2332 of SEQ ID NO: 20. Other forms of B-domain deleted FVIII molecules further have a partial deletion in the a3 domain, resulting in a single chain FVIII molecule.

[0106] This means that these FVIII molecules are recombinant molecules, preferably produced in transformed host cells of mammalian origin. However, the remaining domains in the B-domain deleted FVIII (i.e., the three A domains, the two C domains, and the a1, a2 and a3 regions) may differ slightly, e.g., by about 1%, 2%, 3%, 4% or 5%, from the respective amino acid sequences shown in SEQ ID NO: 20 (amino acids 1-740 and 1649-2332).

[0107] The FVIII molecule used according to the invention may be a two-chain FVIII molecule or a single-chain FVIII molecule. The FVIII molecule used according to the invention may also be a biologically active fragment of FVIII, i.e., FVIII in which domains other than the B domain have been deleted or truncated, but the deleted / truncated form of the FVIII molecule retains the ability to support the formation of blood clots. FVIII activity can be assessed in vitro using techniques well known in the art. A preferred test for determining FVIII activity according to the invention is a chromogenic substrate assay or a one-stage assay (see below). Amino acid modifications (substitutions, deletions, etc.) may be introduced in the remaining domains, for example, to alter the binding ability of factor VIII to various other components such as von Willebrand factor (vWF), low density lipoprotein receptor-related protein (LPR), various receptors, other coagulation factors, cell surfaces, etc., or to introduce and / or eliminate glycosylation moieties, etc. Other mutations that do not eliminate FVIII activity can also be accommodated in the FVIII molecules / analogs for use in the compositions of the invention.

[0108] FVIII analogs also include FVIII molecules in which one or more amino acid residues of the parent polypeptide have been deleted or replaced with other amino acid residues and / or additional amino acid residues have been added to the parent FVIII polypeptide.

[0109] Furthermore, the Factor VIII molecule / analog may contain other modifications, for example in the truncated B domain and / or in one or more of the other domains of the molecule ("FVIII derivatives"). These other modifications may be in the form of various molecules attached to the Factor VIII molecule, such as polymeric compounds, peptidic compounds, fatty acid-derived compounds, etc.

[0110] The term FVIII includes FVIII molecules having a protecting group or a half-life extending moiety. The term "protecting group" / "half-life extending moiety" as used herein includes -SH, -OH, -COOH, -CONH 2, -NH 2 or one or more N- and / or O-glycan structures, which when conjugated to these proteins / peptides, can increase the in vivo circulatory half-life of many therapeutic proteins / peptides. Examples of protecting groups / half-life extending moieties include: biocompatible fatty acids and their derivatives, hydroxyalkyl starches (HAS), e.g., hydroxyethyl starch (HES), poly(Gly ... x -Ser y ) n (Homo Amino Acid Polymer (HAP)), Hyaluronic Acid (HA), Heparosan Polymer (HEP), Phosphorylcholine Polymer (PC Polymer), Fleximer (R) Polymer (Mersana Therapeutics, MA, USA), dextran, polysialic acid (PSA), polyethylene glycol (PEG), Fc domain, transferrin, albumin, elastin-like peptide, XTEN (R) Polymers (Amunix, CA, USA), albumin-binding peptides, von Willebrand factor fragments (vWF fragments), carboxyl-terminal peptides (CTP peptides, Prolor Biotech, IL) and any combination thereof (e.g., McCormick, CL, AB Lowe, and N. Ayres, Water-Soluble Polymers, in Encyclopedia of Polymer Science and Technology. 2002, John Wiley & Sons, Inc.). The method of derivatization is not critical and will be apparent from the above.

[0111] The term FVIII includes glycoPEGylated FVIII. In this context, the term "glycopegylated FVIII" is intended to refer to a factor VIII molecule (including full-length FVIII and B-domain truncated / deleted FVIII) in which one or more PEG groups are attached to a FVIII polypeptide via the polysaccharide side chains (glycans) of the polypeptide.

[0112] FVIII molecules that can be used according to the invention include fusion proteins that contain a FVIII amino acid sequence fused to a heterologous amino acid sequence, preferably a half-life extending amino acid sequence. Preferred fusion proteins are Fc fusion proteins and albumin fusion proteins. The term "Fc fusion protein" is meant herein to encompass FVIII fused to an Fc domain that can be derived from any antibody isotype. IgG Fc domains will often be preferred due to the relatively long circulating half-life of IgG antibodies. The Fc domain can further be modified to modulate specific effector functions, such as complement fixation and / or binding to specific Fc receptors. Fusion of FVIII to an Fc domain capable of binding to the FcRn receptor will generally result in an extended circulating half-life of the fusion protein compared to the half-life of wt FVIII. This means that FVIII molecules for use in the invention can also be derivatives of FVIII analogs, such as, for example, fusion proteins of FVIII analogs, PEGylated or glycoPEGylated FVIII analogs, or FVIII analogs conjugated to heparosan polymers. The term "albumin fusion protein" is meant herein to include FVIII fused to an albumin amino acid sequence or a fragment or derivative thereof. The heterologous amino acid sequence may be fused to the N-terminus or C-terminus of FVIII or may be inserted internally within the FVIII amino acid sequence. The heterologous amino acid sequence may be any of the "half-life extending polypeptides" described in WO2008 / 077616A1, the disclosure of which is incorporated herein by reference.

[0113] Examples of FVIII molecules that can be used according to the present invention are described, for example, in WO2010 / 045568A1, WO2009 / 062100A1, WO2010 / 014708A2, WO2008 / 082669A2, WO2007 / 126808A1, US2010 / 0173831, US2010 / 0173830, US2010 / 0168391, US2010 / 0113365, US2010 / 0113364, WO2003 / 031464A 2, including the FVIII molecules described in WO2009 / 108806A1, WO2010 / 102886A1, WO2010 / 115866A1, WO2011 / 101242A1, WO2011 / 101284A1, WO2011 / 101277A1, WO2011 / 131510A1, WO2012 / 007324A2, WO2011 / 101267A1, WO2013 / 083858A1 and WO2004 / 067566A1.

[0114] Examples of FVIII molecules that can be used in accordance with the present invention include Advate (R) , Helixate (R) , Kogenate (R) , Xyntha (R) , Adynovate (R) , Kovaltry (R) , Novo8 (R) , Nuwiq (R) , Novoeight (R) , Eloctate (R) as well as the FVIII molecules described in WO2008 / 135501A1, WO2009 / 007451A1 and the construct designated "dBN(64-53)" in WO2004 / 067566A1. This construct has the amino acid sequence shown in SEQ ID NO:5.

[0115] "International Unit" or "IU" is a unit of measurement of blood clotting activity (potency) of FVIII as measured by FVIII activity assays such as one-stage clotting assays or chromogenic substrate FVIII activity assays using standards calibrated against international reference specimens calibrated in "IU". One-stage clotting assays are known to those skilled in the art, for example as described in N Lee, Martin L et al., An Effect of Predilution on Potency Assays of FVIII Concentrates, Thrombosis Research (Pergamon Press Ltd.) 30, 511 519 (1983). Principle of the one-stage assay: The test is performed as a modified version of the activated partial thromboplastin time (aPTT) assay: activation of factors of the intrinsic clotting system is induced by incubating plasma with phospholipids and a surface activator. The addition of calcium ions initiates the clotting cascade. The time until a measurable fibrin clot is formed is measured. The assay is performed in the presence of factor VIII-deficient plasma. The clotting ability of factor VIII-deficient plasma is restored by the coagulation factor VIII contained in the sample to be tested. The shortening of the clotting time is proportional to the amount of factor VIII contained in the sample. The activity of coagulation factor VIII is quantified by direct comparison with a standard preparation with known activity of factor VIII in international units. Another standard assay is the chromogenic substrate assay. Chromogenic assays may be purchased commercially, such as the Coamatic FVIII test kit (Chromogenix-Instrumentation Laboratory SpA V.le Monza 338-20128 Milan, Italy). Principle of the chromogenic assay: In the presence of calcium and phospholipids, factor X is activated from factor IXa to factor Xa. This reaction is stimulated by factor VIIIa as a cofactor. FVIIIa is generated from FVIII in the sample to be measured by a small amount of thrombin in the reaction mixture.

[0116] Optimal concentration of Ca 2+When using phospholipids and factor IXa, as well as an excess of factor X, activation of factor X is proportional to the potency of factor VIII. Activated factor X releases the chromophore pNA from the chromogenic substrate S-2765. The amount of pNA released, measured at 405 nm, is therefore proportional to the amount of FXa formed and therefore to the factor VIII activity of the sample.

[0117] When the polypeptide of the present invention and FVIII are used in combination therapy, the ratio of the polypeptide to FVIII administered can be any ratio as defined in the "ratio" section below.In another aspect, the present invention relates to the polypeptide of the present invention for use in increasing the half-life of FVIII in vivo.Yet another aspect of the present invention is a method for increasing the half-life of FVIII in a subject, comprising administering to the subject an effective amount of the polypeptide of the present invention or the pharmaceutical composition of the present invention.The polypeptide of the present invention and FVIII can be co-administered to the subject simultaneously, separately or sequentially.

[0118] Pharmaceutical Compositions Another aspect of the present invention is a pharmaceutical composition comprising a polypeptide of the present invention and, optionally, a pharma- ceutically acceptable carrier, diluent or excipient.In a first embodiment, the pharmaceutical composition comprises the polypeptide of the present invention as the only active ingredient.In a second embodiment, the pharmaceutical composition comprises the polypeptide of the present invention and at least one further active ingredient.Preferably, the further active ingredient is a FVIII molecule as described above.The ratio of the polypeptide of the present invention to FVIII in the pharmaceutical composition can be any ratio as defined in the "Ratio" section below.

[0119] Therapeutic formulations of the polypeptides of the invention can be prepared for storage as frozen formulations or aqueous solutions by mixing the polypeptides of the invention having the desired purity with, optionally, pharma- ceutically acceptable carriers, excipients, or stabilizers (all of which are referred to herein as "carriers") typically used in the art, i.e., buffers, stabilizers, preservatives, isotonicity agents, non-ionic detergents, antioxidants, and various other additives. See Remington's Pharmaceutical Sciences, 16th Edition (Osol, Ed., 1980). Such additives should be non-toxic to subjects at the dosages and concentrations used.

[0120] Buffering agents help maintain pH in a range close to physiologically acceptable conditions. It can typically be present at a concentration ranging from about 2 mM to about 100 mM. Suitable buffering agents include both organic and inorganic acids and their salts, such as citrate buffers (e.g., monosodium citrate-disodium citrate mixtures, citric acid-trisodium citrate mixtures, citric acid-monosodium citrate mixtures, etc.), succinate buffers (e.g., succinic acid-monosodium succinate mixtures, succinic acid-sodium hydroxide mixtures, succinic acid-disodium succinate mixtures, etc.), tartrate buffers (e.g., tartaric acid-sodium tartrate mixtures, tartaric acid-potassium tartrate mixtures, tartaric acid-sodium hydroxide mixtures, etc.), fumarate buffers (e.g., fumaric acid-monosodium fumarate mixtures, fumaric acid-monosodium fumarate mixtures, fumaric acid-monosodium fumarate mixtures, etc.), and the like. Examples of suitable buffers include: oxalic acid-disodium fumarate mixtures, monosodium fumarate-disodium fumarate mixtures, etc.), gluconic acid buffers (gluconic acid-sodium gluconate mixtures, gluconic acid-sodium hydroxide mixtures, gluconic acid-potassium gluconate mixtures, etc.), oxalic acid buffers (e.g., oxalic acid-sodium oxalate mixtures, oxalic acid-sodium hydroxide mixtures, oxalic acid-potassium oxalate mixtures, etc.), lactate buffers (lactate-sodium lactate mixtures, lactate-sodium hydroxide mixtures, lactate-potassium lactate mixtures, etc.), and acetate buffers (acetic acid-sodium acetate mixtures, acetic acid-sodium hydroxide mixtures, etc.). Additionally, phosphate buffers, histidine buffers, trimethylamine salts such as Tris can be used.

[0121] Preservatives can be added to retard microbial growth, typically in amounts ranging from 0.2% to 1% (w / v). Suitable preservatives include phenol, benzyl alcohol, meta-cresol, methylparaben, propylparaben, octadecyldimethylbenzylammonium chloride, benzalkonium halides (e.g., chloride, bromide, iodide), hexamethonium chloride, and alkylparabens such as methyl or propylparaben, catechol, resorcinol, cyclohexanol, and 3-pentanol.

[0122] Osmolality adjusting agents, often known as "stabilizers," can be added to ensure pharma- ceutically acceptable osmolality, preferably isotonicity, of liquid compositions and include polyhydric sugar alcohols, preferably trihydric or higher sugar alcohols, such as glycerin, erythritol, arabitol, xylitol, sorbitol, and mannitol, as well as inorganic salts such as sodium chloride. Stabilizers refer to a broad category of excipients whose functions can range from bulking agents to additives that solubilize therapeutic agents or help prevent denaturation or adhesion to container walls. Exemplary stabilizers include polyhydric sugar alcohols (listed above); amino acids such as arginine, lysine, glycine, glutamine, asparagine, histidine, alanine, ornithine, L-leucine, 2-phenylalanine, glutamic acid, threonine, and the like; organic sugars or sugar alcohols such as lactose, trehalose, stachyose, mannitol, sorbitol, xylitol, ribitol, myo-inositol, galactitol, glycerol, and the like, including cyclitols such as inositol; polyethylene glycols; amino acid polymers; sulfur-containing reducing agents such as glutamine, glycerol ... Stabilizers can be cation, thioctic acid, sodium thioglycolate, thioglycerol, α-monothioglycerol and sodium thiosulfate; low molecular weight polypeptides (e.g., peptides of 10 residues or less); proteins, such as human serum albumin, bovine serum albumin, gelatin or immunoglobulins; hydrophilic polymers, such as polyvinylpyrrolidone, monosaccharides, such as xylose, mannose, fructose, glucose; disaccharides, such as lactose, maltose, sucrose and trisaccharides, such as raffinose; and polysaccharides, such as dextran. Stabilizers can be present in the range of 0.1 to 10,000 parts by weight per part by weight of the polypeptide of the invention. Non-ionic surfactants or detergents (also known as "wetting agents") can be added to aid in solubilizing the therapeutic agent as well as to protect the therapeutic protein from agitation-induced aggregation, which also allows the formulation to be exposed to shear surface stresses without causing denaturation of the protein.Suitable non-ionic surfactants include polysorbates (20, 80, etc.), poloxamers (184, 188, etc.), pluronic polyols, polyoxyethylene sorbitan monoethers (TWEEN. (R) -20, TWEEN (R) -80, etc. The non-ionic surfactant can be present in a range of about 0.05 mg / ml to about 1.0 mg / ml, or in a range of about 0.05 mg / ml to about 0.2 mg / ml.

[0123] Additional miscellaneous excipients include bulking agents (eg, starch), chelating agents (eg, EDTA), antioxidants (eg, ascorbic acid, methionine, vitamin E), and cosolvents.

[0124] Freeze-drying or lyophilization shall be used to indicate a drying process that converts solutions of substances (i.e., active pharmaceutical ingredients and various formulation additives or "excipients") into solids, unless otherwise indicated by the context in which it appears. A typical freeze-drying process consists of three stages: "freezing", "primary drying" and "secondary drying". The freezing stage converts almost all contained water into ice and solutes into solids (crystalline or amorphous). The primary drying stage removes ice from the product by direct sublimation by maintaining a favorable pressure gradient between the water molecules (ice) and the surrounding atmosphere. The secondary drying stage removes residual moisture from the product by desorption.

[0125] When a concentration (w / v) of a lyophilized composition is given, it refers to the volume immediately prior to lyophilization.

[0126] Unless otherwise stated, percentage terms refer to weight / weight percentages and temperatures are in degrees Celsius.

[0127] ratio As described in more detail below, the polypeptides of the invention may be monomers, dimers, or mixtures thereof, Any molar ratio according to the invention refers to the molar ratio of dimers of the polypeptides of the invention, regardless of whether they actually exist as homodimers or heterodimers.

[0128] Any ratio of the polypeptide of the invention to FVIII in this application refers to the amount of dimer (in moles) contained in the polypeptide of the invention, which is preferably present as a heterodimer, divided by the amount of FVIII (in moles), unless otherwise stated. As a non-limiting example, a co-formulation of 100 μM of a heterodimeric polypeptide of the invention consisting of 200 μM monomeric subunits with 1 μM FVIII means a ratio of 100.

[0129] The molar ratio of the polypeptide of the invention to FVIII may be at least 1, preferably at least 2, more preferably at least 4, or at least 10, or at least 20, or at least 25, or at least 50, or more than 50, or at least 100, or at least 150, or at least 200, or at least 250, or at least 300, or at least 350, or at least 400, or at least 450, or at least 500, or at least 1,000, or at least 1,500, or at least 2,500, or at least 4,000, or up to 5,000. The molar ratio of the polypeptide of the invention to FVIII may according to certain embodiments not exceed a ratio of 5,000, a ratio of 2,500, a ratio of 1,250, or a ratio of 1,000.

[0130] The molar ratio of the polypeptide of the present invention to FVIII may be in the range of about 1 to 5,000, or 2 to 2,500, or 4 to 2,000, or 10 to 1,500, or 25 to 1,000, or 50 to 500. Preferably, the molar ratio of the polypeptide of the present invention to FVIII is in the range of 1 to 1,250, or 2 to 1,000, or 4 to 750, or 10 to 500.

[0131] The above ratios refer to the ratio administered during combined treatment or to the ratio of both active ingredients in a pharmaceutical composition.

[0132] The nucleotide and amino acid sequences shown in the sequence listing are summarized in Table 1.

[0133] [Table 1]

[0134] Certain embodiments of the present invention will now be described with reference to the following examples, which are for illustrative purposes only and are not intended to limit the scope of the concepts described herein.

[0135] Working Example result D'D3-TER119 dimers cross-link murine RBCs in vitro.

[0136] To extend the half-life of FVIII co-administered for the treatment of hemophilia A, we previously generated a recombinant VWF D'D3 albumin fusion protein (rD'D3-FP) (reference paper, A245). We hypothesized that a fusion of D'D3-FP with TER119scFv (TER119-specific single-chain Fv; an antibody fragment with specificity for mouse glycophorin A) could function as a mediator to target FVIII to RBCs in situ due to its dual specificity. rD'D3-FP fused with TER119scFv (D'D3-FP-TER119scFv, SEQ ID NO: 3) was stably expressed along with the VWF propeptide (D1D2), which is essential for efficient dimerization of the D'D3 domain (Figure 1). CHO expressing cell lines secreted monomers and homodimers of D'D3-FP-TER119scFv as expected (Figure 2). Both species were purified separately. FVIII (rVIII-SingleChain, CSL Behring, Marburg, Germany) could indeed be detected on the surface of RBCs spotted with both monomer and homodimer of D'D3-FP-TER119scFv in the presence of 5 μg / ml FVIII. RBCs were efficiently stained by polyclonal anti-FVIII-FITC with the homodimer but not the monomer (data not shown). However, at physiologically relevant concentrations in vitro (>5 μg / ml), it was observed that RBCs formed visible clumps with the homodimer but not with the monomer. Surprisingly, the monomer only cross-linked RBCs in the presence of a detection antibody against human albumin (Figure 3). We considered this finding as a potential safety risk in in vivo applications.

[0137] It has been previously demonstrated that FVIII is efficiently bound by D'D3 dimers, but not by monomeric D'D3. Monomeric D'D3 has a significantly reduced affinity for its natural ligand, FVIII, compared to D'D3 dimers (WO2018 / 087271A1). FVIII-RBC interactions would be further inhibited by endogenously circulating VWF in an in vivo scenario. We have previously demonstrated that dimeric D'D3-FP extends the half-life of FVIII only when administered at a dose that competes with and exceeds the molar concentration of endogenous VWF in plasma. Due to the low affinity of D'D3 monomers for FVIII, they would need to be injected at a significantly higher dose than D'D3 dimers. Thus, overcoming the issue of cross-linked RBCs through the development of monomeric D'D3-FP-TER119scFv was not an option for us. Dimerized D'D3 was preferred as a subunit of the bispecific molecule.

[0138] The D'D3-TER119 heterodimer promotes FVIII binding to mouse RBCs without cross-linking in vitro.

[0139] Surprisingly, D'D3-FP-TER119scFv (SEQ ID NO: 3) and D'D3 (without albumin and TER119scFv fusion partners, SEQ ID NO: 5) co-expressed in a stably transfected cell line were secreted as heterodimers and could be purified using two affinity steps against human albumin and a tag at the c-terminus of the unfused D'D3 (Figures 4 and 5). The same expression strategy was used to engineer the previously published D'D3 known as "D'D3 EYA" or CSL629 (WO2017 / 117630A1, WO2017 / 117631A1; SEQ ID NO: 14). EYA -FP-TER119scFv (SEQ ID NO: 7) and D'D3 EYA(SEQ ID NO: 9). Figure 6 shows that D'D3-FP-TER119scFv promotes human FVIII binding to mouse RBCs in vitro in a concentration-dependent manner, both as wild type (WT) and as a high affinity variant (EYA). Briefly, a constant concentration of rVIII-SingleChain (12.5 IU / mL, approximately 6 nM, CSL Behring Marburg) was added to purified heterodimeric constructs, D'D3 WT -TER119 and D'D3 EYA After incubation with increasing concentrations of D'D3-TER119, washed mouse RBCs from fresh blood diluted 1:100 were added and analyzed. FVIII on the mouse RBC surface was specifically detected by a polyclonal anti-human FVIII FITC-labeled antibody. Importantly, the bispecific but monovalent design of the D'D3-TER119 heterodimer did not show any aggregation or cross-linking of mouse RBCs, either visually or based on SSC / FSC (single cell) analysis. Human plasma-derived VWF (12.5 IU / ml, approximately 250 nM, CSL Behring, Marburg) was added as indicated. Thus, according to the definition of the plasma unit, the molar ratio of FVIII to VWF was 1 to 35, which was assumed to be physiologically relevant. An equal, fixed standard plasma unit concentration of FVIII and VWF (12.5 IU / ml) was chosen while titrating the D'D3-TER119 heterodimer construct to simulate in vitro the competition of both D'D3-TER119 and endogenous VWF for the common ligand, FVIII. WT -TER119 inhibits VWF from binding to mouse RBCs, whereas D'D3 EYA This is because human VWF and D'D3WT-TER119 have the same amino acid sequence of their FVIII-binding subunit, D'D3, and therefore have equal affinity for FVIII (WO2017 / 117630A1, WO2017 / 117631A1). WT -TER119 does not promote FVIII binding to mouse RBCs in the presence of human VWF, D'D3EYA -TER119 is able to target FVIII even at a lower molar number than that of VWF, an observation that can be explained by the 30-fold higher affinity of the D'D3(EYA)-FP(CSL629) variant compared to wild-type D'D3-FP(CSL626) or VWF for FVIII (WO2017 / 117630A1, WO2017 / 117631A1).

[0140] [Table 2]

[0141] The apparent KM of the binding kinetics of FVIII bound to D'D3EYA-TER119 to mouse RBCs was calculated assuming Michaelis-Menten kinetics (Figure 6B). A summary of the KM calculations for two independent in vitro experiments is provided in Table 2. One of the in vitro experiments showed that the presence of VWF significantly increased the binding of D'D3EYA-TER119 to mouse RBCs compared to data without VWF (17.8 nM ± 0.5%). EYA These results suggest that the KM for FVIII binding to RBCs using -TER119 (32.4 nM ± 0.7%) was increased. This observation did not confirm any qualitative trend (Figure 6A). Furthermore, the two batches of D'D3 EYA When the experiment was repeated with -TER119, no significant differences in KM were observed in the absence or presence of VWF (3.7 nM ± 5.7% and 3.6 nM ± 0.2%). EYA The second batch of -TER119 was observed to have an increased KM (9.3 nM ± 8.3% and 9.9 nM ± 2.0%) compared to the first batch of material, both with and without VWF, however the trend of the titration curve (up to 102 nM) was not representative of that observation.

[0142] D'D3 WT -TER119 titration is D'D3 without VWF EYAThe results show an increased KM (25.6 nM ± 4%) compared to the KM of -TER119, but again the trend in Figure 6A does not reflect this observation. EYA -TER119 already shows maximum binding, whereas FVIII binding on RBCs is D'D3 WT -Only weakly detected at high concentrations of TER119 (4-12.5 nM), and D'D3 with VWF WT The KM of D'D3-TER119 could not be calculated. Because these in vitro experiments used a fixed molar excess VWF concentration of approximately 250 nM that competes with the D'D3-TER119 variant for the common binding partner FVIII, our flow cytometry binding studies confirm the binding affinity of the high affinity variant of the bispecific heterodimer, D'D3 EYA The results clearly demonstrated the beneficial effect of targeting FVIII to RBCs with -TER119.

[0143] Reduction of FVIII antibodies in FVIII ko mice treated with D'D3-TER119 heterodimer Weekly administration of rFVIII is known to result in anti-drug antibody (ADA) formation, typically shown to be inhibitory antibodies (measured in Bethesda units) in FVIII ko mice. In this example, we investigated the role of D'D3 in the development of ADA against rFVIII, rVIII-Single Chain. EYA The effect of the -TER119 heterodimer was examined in comparison to a non-erythrocyte binding control, D'D3(EYA)-FP.

[0144] It was reported that coadministration of recombinant FVIII (rVIII-SingleChain, 200IU / kg, approximately 16μg / kg) with 100μg / ml of D'D3(EYA)-FP(CSL629), previously published as CSL629, extended the half-life of infused FVIII in vivo (WO2018 / 087271A1). As one explanation for the mechanism of action, it has been argued that a molar ratio of at least 1:4 between FVIII and its high affinity chaperone, D'D3(EYA)-FP(CSL629), was sufficient to capture the majority of infused FVIII molecules into the circulation. Thus, it was shown that a small fraction of infused FVIII bound to endogenous VWF and not to CSL629, which could still rescue FVIII ko mice in a bleeding model (data not shown). We applied the same molar ratio (1:4) but at approximately 10-fold higher doses in vivo to induce tolerance to FVIII. For the initial dosing regimen, we also considered injecting less than 1 mg / kg of human heterologous protein to avoid anaphylaxis.

[0145] rVIII-SingleChain (2000IU / kg) D'D3 EYA Administration of rVIII-SingleChain with or without D'D3EYA-TER119 heterodimer (840 μg / kg) to n=10 FVIII ko mice / group on days 0, 7, 14, 21 and 28 (see Figure 7) induced the production of anti-FVIII-ADA and inhibitory antibodies neutralizing FVIII activity. Anti-FVIII ADA values ​​are given as sum of attenuations and are shown as individual values ​​and mean ± SD (Figure 8A). Administration of rVIII-SingleChain at a dose of 2000 IU / kg alone to FVIII ko mice induced the production of 6.61 ± 1.14 ADA on day 35. Co-administration of D'D3EYA-TER119 heterodimer at a dose of 840 μg / kg using two different batches significantly reduced the sum of attenuations to 2.88 ± 1.04 (batch 1) and 3.04 ± 0.82 (batch 2). Co-administration of the two D'D3EYA-TER119 heterodimer at a dose of 840 μg / kg significantly reduced the sum of attenuations to 2.88 ± 1.04 (batch 1) and 3.04 ± 0.82 (batch 2). EYA There were no significant differences between the -TER119 heterodimer batches (Figure 8A).

[0146] The ADA generated against FVIII was quantified in Bethesda units and shown to be inhibitory with roughly the same response, as can be seen by plotting for each individual animal and the mean ± SD (Figure 8B). Furthermore, the ADA value (sum of attenuation) for each individual was compared to the inhibitory potential quantified in Bethesda units, and a linear regression was calculated across all values, yielding an r of 0.55. 2 values ​​were obtained (FIG. 8C), supporting a direct correlation between the sum of anti-FVIII ADA attenuation and its inhibitory potential measured as Bethesda units.

[0147] In conclusion, two independent batches of D'D3EYA-TER119 heterodimer reduced the immunogenicity of rVIII-SingleChain in FVIII ko mice.

[0148] D'D3EYA-TER119 induces long-term tolerance to FVIII in FVIII ko mice.

[0149] We were also interested in whether the D'D3EYA-TER119 heterodimer was effective in inducing long-term tolerance to FVIII. (R)D'D3EYA-TER119 (672 μg / kg, approximately 4-fold molar excess compared to FVIII) was injected intravenously (iv) once a week for 4 weeks with or without D3EYA-TER119 (672 μg / kg, approximately 4-fold molar excess compared to FVIII). One week after the fourth injection, an intermediate bleed was performed and plasma was analyzed for anti-FVIII antibodies by ELISA. After a further 4 weeks, mice were rechallenged with FVIII alone (120 IU / kg, iv) for 2 weeks. Seven days after the last injection of FVIII, mice were euthanized for collection of terminal plasma and plasma was analyzed for anti-FVIII antibodies by ELISA. Surprisingly, administration of the D'D3EYA-TER119 heterodimer in combination with FVIII significantly reduced antibody formation against FVIII and did not result in a significant increase in anti-FVIII antibody levels after rechallenge.

[0150] [Table 3]

[0151] [Table 4]

Claims

1. A polypeptide comprising (i) a VWF portion and (ii) an erythrocyte-binding portion, wherein the polypeptide is capable of binding to blood coagulation factor VIII (FVIII), the VWF portion is capable of binding to FVIII, the VWF portion is a truncated VWF comprising the D'D3 domain of VWF, the erythrocyte-binding portion is selected from the group consisting of an antibody, an antibody fragment, and a single-chain antigen-binding domain (scFv), the polypeptide is a heterodimer, the heterodimer comprising a first subunit and a second subunit, the first subunit comprising a first of the VWF portions and the erythrocyte-binding portion, the second subunit comprising a second of the VWF portions, and the second subunit not comprising the erythrocyte-binding portion.

2. The polypeptide of claim 1 , wherein the VWF portion comprises at least one amino acid substitution compared to the amino acid sequence of wild-type VWF as set forth in SEQ ID NO:

18.

3. The polypeptide of claim 2, wherein the at least one amino acid substitution is selected from the group of combinations consisting of S764G / S766Y, S764P / S766I, S764P / S766M, S764V / S766Y, S764E / S766Y, S764Y / S766Y, S764L / S766Y, S764P / S766W, S766W / S806A, S766Y / P769K, S766Y / P769N, S766Y / P769R, S764P / S766L, and S764E / S766Y / V1083A, with reference to the sequence of SEQ ID NO:18 in terms of amino acid number.

4. The polypeptide according to any one of claims 1 to 3, wherein the erythrocyte binding moiety is capable of binding to a membrane protein on an erythrocyte.

5. A pharmaceutical composition comprising a polypeptide according to any one of claims 1 to 4, optionally together with a pharma- ceutically acceptable carrier, diluent or excipient.

6. A pharmaceutical composition according to claim 5 for use in therapy or as a medicament. 。

7. 6. The pharmaceutical composition of claim 5 for use in the treatment of a blood clotting disorder.

8. The pharmaceutical composition for use according to claim 7, wherein said treatment comprises administration of FVIII.

9. The pharmaceutical composition for use according to claim 8 , wherein the polypeptide and FVIII are co-administered.

10. 6. The pharmaceutical composition according to claim 5 for use in increasing the in vivo half-life of FVIII.

11. 6. A pharmaceutical composition according to claim 5 for use in preventing or reducing inhibitor formation during treatment with FVIII.

12. A nucleic acid encoding the polypeptide according to any one of claims 1 to 4.

13. A plasmid or vector comprising the nucleic acid of claim 12.

14. A host cell comprising the plasmid or vector of claim 13.

15. A method for producing a polypeptide comprising VWF and an erythrocyte-binding portion, comprising: (i) culturing a host cell of claim 14 under conditions such that a polypeptide comprising VWF and an erythrocyte-binding portion is expressed; and (ii) optionally recovering the polypeptide comprising VWF and an erythrocyte-binding portion from the host cell or culture medium.

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