Peptides having hair loss prevention or hair growth promoting activity and their uses

Abstract

The present invention relates to a peptide having a novel sequence that has the activity of promoting hair growth and preventing hair loss. Since the peptide has the activity of promoting or inducing the proliferation or activity of cells involved in hair formation, a composition containing the peptide can be usefully used for preventing hair loss or promoting hair growth.

Description

[Technical Field]

[0001] The present invention relates to a novel peptide having an activity capable of improving hair loss or promoting hair growth, and uses thereof. [Background technology]

[0002] The average human body has approximately 100,000 to 150,000 hairs. As cell division and activity in hair follicles cease during the process of repeated growth, regression, and rest, existing hairs are pushed out by newly growing hairs, causing them to naturally fall out. This natural hair loss phenomenon occurs when an average of approximately 100 hairs fall out per day. If more hair falls out than the natural hair loss described above, or if hair does not exist or does not grow in areas where hair normally should be, it becomes a type of hair loss disorder. Hair loss can occur as a natural part of the aging process, but it can also be caused by external environmental factors such as hormonal changes, stress, air pollution, and processed foods.

[0003] As hair loss progresses, the hair papilla at the hair root shrinks, causing the thickness of the hair growing from the hair papilla to decrease. The hair cycle (growth, regression, and resting) also shortens, resulting in thinner newly growing hairs. As this phenomenon repeats, the gradually thinner hair turns into fluff, and the hair cycle gradually shortens, causing hair to stop growing and fall out, resulting in a decrease in the overall number of hairs. While hair loss was previously thought of as a phenomenon or disease primarily affecting older men, statistics show that nearly half of hair loss patients are young people in their 20s and 30s, and the number of female patients is gradually increasing. Because hair loss induces significant changes in the external appearance, the cost of hair loss treatment, such as undergoing transplant surgery or taking medication, is gradually increasing among young people who are concerned about beauty and appearance.

[0004] Despite this, research and development of effective treatments for hair loss remains insufficient. Korean Patent Publication No. 2020-0058932 discloses a composition for preventing and improving hair loss containing platycodon extract, and Korean Patent Publication No. 2017-0046528 discloses a complex composition for improving and preventing hair loss containing various herbal extracts. However, the use of a wide variety of natural products containing diverse ingredients can lead to unpredictable side effects, and hormone-regulating treatments in particular have the disadvantage of causing side effects related to sexual function or being inapplicable to women. Meanwhile, if a peptide effective in treating hair loss is developed and used as a hair loss treatment among peptides formed by linking amino acids, it has the advantage of being highly biocompatible and potentially mimicking the active sites of other proteins in the human body and exhibiting similar activity. Furthermore, the use of a single peptide has a clearer mechanism of action than extracts containing various ingredients, and is easily degraded and removed by biodegradable proteins in the body after locally inhibiting hair loss, thereby reducing the risk of side effects compared to existing treatments. Therefore, there is an urgent need to develop peptide preparations that can effectively prevent hair loss and promote hair growth while minimizing side effects. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Korean Patent Publication No. 2020-0058932 [Patent Document 2] Korean Patent Publication No. 2017-0046528 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a peptide that has the activity of inducing an increase in the proliferation or activity of cells involved in hair formation and is capable of promoting hair growth.

[0007] Another object of the present invention is to provide a composition for effectively preventing, treating, or improving hair loss or promoting hair growth using the above peptides. [Means for solving the problem]

[0008] To achieve the above object, one aspect of the present invention provides a peptide having an activity of preventing hair loss or promoting hair growth, which comprises the amino acid sequence of SEQ ID NO: 1.

[0009] Another aspect of the present invention provides a composition for preventing hair loss or promoting hair growth, comprising the peptide.

[0010] Another aspect of the present invention provides a pharmaceutical composition for preventing or treating hair loss, comprising the peptide.

[0011] Another aspect of the present invention provides a cosmetic composition for preventing or improving hair loss, comprising the peptide.

[0012] The present invention will be described in detail below.

[0013] 1. Peptides with hair loss prevention or hair growth promotion activity and their uses One aspect of the present invention provides novel peptides that have the activity of preventing hair loss or promoting hair growth.

[0014] In the present invention, the term "peptide" means a polymer of two or more amino acids linked by peptide bonds.

[0015] In the present invention, the term "hair loss" refers to the absence of hair in areas where hair normally exists, and includes a phenomenon in which the number of hairs is reduced compared to normal. The term "hair loss" encompasses both non-cicatricial and cicatricial alopecia depending on whether or not hair follicles are destroyed, and includes, for example, hereditary androgenetic alopecia, alopecia areata, tinea capitis due to fungal infection, telogen effluvium, trichotillomania, hair loss due to hair production disorders or lupus, folliculitis barbae, lichen planus pilaris, and hair loss due to burns and / or trauma.

[0016] The peptide comprises the amino acid sequence of SEQ ID NO: 1. The peptide may include a variant peptide having a different sequence due to deletion, insertion, substitution, or a combination thereof of amino acid residues, provided that the activity of the peptide is not affected, or may be in the form of a protein fragment having the same function. Amino acid modifications at the protein and peptide level that do not overall alter the activity of the amino acid sequence of SEQ ID NO: 1 are known in the art, and may include modifications such as phosphorylation, sulfation, acrylation, glycosylation, methylation, farnesylation, etc., in some cases. Therefore, the peptide includes not only the amino acid sequence of SEQ ID NO: 1, but also peptides having substantially the same amino acid sequence or variants thereof. The peptide having substantially the same amino acid sequence may be a peptide containing an amino acid sequence having 90% or more, 91% or more, 92% or more, 93% or more, 94% or more, 95% or more, 96% or more, 97% or more, 98% or more, 99% or more, or 99.5% or more homology to the amino acid sequence of SEQ ID NO: 1, but is not limited thereto. Any peptide having an amino acid sequence having 90% or more homology to the amino acid sequence of SEQ ID NO: 1 and having the same activity is included within the scope of the present invention.

[0017] The peptide of the present invention may be composed of 20 or less amino acids that exhibit anti-hair loss or hair growth promoting activity while containing the amino acid sequence of SEQ ID NO: 1. Specifically, the peptide may be composed of 20 or less, 18 or less, 15 or less, or 12 amino acids.

[0018] The peptides of the present invention can be obtained by various methods widely known in the art, including, but not limited to, in vitro synthesis using polynucleotide recombination and a protein expression system, chemical synthesis such as peptide synthesis, and cell-free protein synthesis.

[0019] In addition, a protecting group may be attached to the N- or C-terminus of the peptide to achieve better chemical stability, enhanced pharmacological properties (e.g., half-life, water absorption, potency, efficacy), altered specificity (e.g., a broader spectrum of biological activity), or reduced antigenicity. For example, the protecting group may be an acetyl group, a fluorenylmethoxycarbonyl group, a formyl group, a palmitoyl group, a myristyl group, a stearyl group, or a polyethylene glycol (PEG). However, any component that can enhance peptide modification, particularly peptide stability, may be included without limitation. The term "stability" refers not only to in vivo stability, which protects the peptide of the present invention from attack by in vivo protease enzymes, but also to storage stability (e.g., room temperature storage stability).

[0020] The peptide of the present invention may promote the proliferation or activity of cells involved in hair formation. Specifically, the peptide may have the activity of promoting the proliferation of hair formation-related cells, such as dermal papilla cells, outer root sheath cells, and hair matrix cells, thereby increasing their number, or may have the activity of inducing hair formation by promoting the activity of these cells.

[0021] Furthermore, the peptide of the present invention may suppress the expression of proteins involved in the occurrence of hair loss in cells involved in hair formation, specifically, may suppress the expression of DKK-1 protein (Dickkopf-related protein 1) in hair papilla cells, and more specifically, may suppress the expression of DKK-1 protein in hair papilla cells in which the expression of DKK-1 protein has been induced by a hormone such as DHT (dihydrotestosterone).

[0022] The peptide of the present invention has the above-mentioned activity and can therefore be useful for preventing hair loss or promoting hair growth.

[0023] Accordingly, another aspect of the present invention provides a composition for preventing hair loss or promoting hair growth, comprising the peptide.

[0024] The peptide may be contained in the composition at a concentration of 0.01 μM to 100 μM. Specifically, the peptide may be contained at a concentration of 0.01 μM to 100 μM, 0.05 μM to 80 μM, 0.1 μM to 60 μM, or 0.5 μM to 50 μM, but is not limited thereto.

[0025] When the concentration of the peptide contained in the composition of the present invention is within the above range, the activity of the peptide is sufficient to prevent hair loss and / or promote hair growth, while having the advantage of not being toxic to living organisms or their cells.

[0026] The composition may induce hair formation by promoting the activity of one or more cells selected from the group consisting of dermal papilla cells, outer root sheath cells, and hair matrix cells.

[0027] The composition may induce phosphorylation of one or more selected from the group consisting of AKT and ERK in dermal papilla cells. Specifically, the composition can increase the amount of p-AKT and / or p-ERK in dermal papilla cells, thereby activating a signal transduction pathway related to dermal papilla cell proliferation.

[0028] The composition may induce activation of β-catenin in dermal papilla cells. Specifically, the composition can activate β-catenin in dermal papilla cells and induce its translocation to the nucleus, thereby promoting or inducing cell proliferation and / or differentiation of dermal papilla cells.

[0029] The composition may promote the expression of one or more selected from the group consisting of LEF-1, c-Myc, and cyclin D1 in dermal papilla cells. Specifically, the composition can promote the expression of LEF-1, c-Myc, and / or cyclin D1 in dermal papilla cells by promoting or inducing cell proliferation and / or differentiation.

[0030] In a specific embodiment of the present invention, compositions containing the peptides of the present invention were applied to human hair follicle dermal papilla cells (HHFDPCs) at various concentrations, and the absorbance was measured. The absorbance was higher than that of the control group, confirming the effect of promoting and inducing HHFDPC proliferation. Furthermore, in the HHFDPCs, p-AKT and p-ERK, factors related to proliferation and activation, increased, and β-catenin was activated and its translocation to the nucleus increased, resulting in increased expression of LEF-1, c-Myc, and cyclin D1. Therefore, it was confirmed that the peptides of the present invention and compositions containing them have excellent effects of promoting hair growth or preventing hair loss by promoting and inducing the proliferation and activity of dermal papilla cells, the cells that make up hair follicles.

[0031] The composition may suppress the expression of DKK-1 protein in dermal papilla cells, specifically in dermal papilla cells in which the expression of DKK-1 protein has been induced by a hormone such as DHT (dihydrotestosterone).

[0032] In a specific embodiment of the present invention, compositions containing the peptides of the present invention were applied at various concentrations to HHFDPC dermal papilla cells in which DKK-1 protein expression had been induced by treatment with DHT, and the DKK-1 protein was examined by Western blotting. As a result, it was confirmed that the amount of DKK-1 protein was reduced compared to the negative control group, demonstrating that the peptides of the present invention and compositions containing the same have the effect of suppressing the expression of hair loss-related proteins in dermal papilla cells and can be useful for preventing hair loss.

[0033] The composition may promote the expression of one or more selected from the group consisting of Ha3-II, Keratin 14, and Keratin 19 in outer root sheath cells. Specifically, the composition may promote the expression of Ha3-II, Keratin 14, and / or Keratin 19 by increasing the cellular activity of outer root sheath cells.

[0034] In a specific embodiment of the present invention, compositions containing the peptide of the present invention were treated at different concentrations with outer root sheath cells (HHORSCs), and the expression of Ha3-II, Keratin 14, and Keratin 19 was confirmed by RT-PCR. As a result, it was confirmed that the expression levels were increased compared to the control group. This confirmed that the peptide of the present invention and compositions containing the same have the effect of increasing the activity of outer root sheath cells, a type of cell that makes up hair follicles, and therefore have the effect of preventing hair loss or promoting hair growth.

[0035] The composition may promote the expression of MSX2 in hair matrix cells. Specifically, the composition may promote the expression of MSX2, a transcription factor involved in the cellular activity of hair matrix cells, to induce the expression of genes associated with hair matrix cell activity.

[0036] In a specific embodiment of the present invention, compositions containing the peptide of the present invention were treated at different concentrations with hair matrix cells (HHGMC) and the expression of MSX2 was confirmed via RT-PCR. As a result, it was confirmed that the expression level increased compared to the control group. This confirmed that the peptide of the present invention and compositions containing it have the effect of increasing the activity of hair matrix cells, a type of cell that forms hair, and thus have the effect of preventing hair loss or promoting hair growth.

[0037] 2. Pharmaceutical composition for preventing or treating hair loss Yet another aspect of the present invention provides a pharmaceutical composition for preventing or treating hair loss, comprising the peptide.

[0038] The peptide comprises the amino acid sequence of SEQ ID NO: 1, and the description thereof is the same as that of the peptide described in the section "Peptides having hair loss prevention or hair growth promotion activity and their uses," so the detailed description is incorporated herein by reference. Hereinafter, only the specific components of the pharmaceutical composition will be described. In the present invention, the term "prevention" means reducing the risk of contracting a disease or disorder, and refers to any action that suppresses or delays the onset of a disease by preventing the progression of one or more clinical symptoms of the disease in a subject who is exposed to or susceptible to contracting a disease but has not yet contracted the disease or shown symptoms of the disease.

[0039] In the present invention, the term "treatment" means alleviating a disease or disorder and includes any action that prevents or reduces the progression of a disease or one or more of its clinical symptoms, thereby improving or otherwise altering the symptoms of the disease.

[0040] In the pharmaceutical composition of the present invention, the peptide may be contained in a therapeutically effective amount, and may further contain a pharmaceutically acceptable carrier. The term "therapeutically effective amount" means an amount sufficient to achieve the purpose of the composition, which is to prevent or treat hair loss.

[0041] In the present invention, hair loss refers to the absence of hair in areas where hair normally should be present, and includes a phenomenon in which the number of hairs is reduced compared to normal. The concept of hair loss encompasses both non-cicatricial and cicatricial alopecia depending on whether hair follicles are destroyed, and includes, for example, hereditary androgenetic alopecia, alopecia areata, tinea capitis due to fungal infection, telogen effluvium, trichotillomania, hair loss due to hair production disorders or lupus, folliculitis barbae, lichen planus pilaris, and hair loss due to burns and / or trauma.

[0042] The prevention or treatment of hair loss may be to remove the cause of the hair loss or to inhibit the progression of hair loss, or may be to inhibit hair loss or to promote hair formation to promote hair growth.

[0043] Meanwhile, the pharmaceutical composition of the present invention may be prepared in a unit dose form or in a multi-capacity container by formulating it with pharmaceutically acceptable carriers and / or excipients by a method easily performed by a person skilled in the art to which the present invention pertains. In this case, the dosage form may be a solution, suspension, or emulsion in an oil or aqueous medium, or an extract, powder, granules, tablets, capsules, or gel (e.g., hydrogel), and may further contain a dispersant or stabilizer.

[0044] The peptides contained in the pharmaceutical compositions may be delivered in pharmaceutically acceptable carriers such as colloidal suspensions, powders, saline solutions, lipids, liposomes, microspheres, or nanospheres, which may be complexed or associated with delivery vehicles and delivered in vivo using delivery systems known in the art, such as lipids, liposomes, microparticles, gold, nanoparticles, polymers, condensation reagents, polysaccharides, polyamino acids, dendrimers, saponins, adsorption enhancers, or fatty acids.

[0045] In addition, pharmaceutically acceptable carriers may include, but are not limited to, commonly used ingredients in pharmaceutical formulations, such as lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition to the above ingredients, the formulation may further include lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives, etc.

[0046] The pharmaceutical composition of the present invention can be administered orally or parenterally during clinical administration and may be used in the form of a common pharmaceutical formulation. That is, the pharmaceutical composition of the present invention can be administered in various oral and parenteral dosage forms during clinical administration. When formulated, it is formulated using commonly used diluents or excipients, such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like. These solid formulations are formulated by mixing herbal extracts or fermented herbal products with at least one or more excipients, such as starch, calcium carbonate, sucrose or lactose, and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral administration include suspensions, oral solutions, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients, such as wetting agents, sweeteners, flavoring agents, and preservatives, may be included. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. Suppository bases may include witepsol, macrogol, Tween 61, cocoa butter, laurin butter, glycerol, gelatin, and the like.

[0047] The dosage of the peptide may vary depending on factors such as formulation method, administration method, age, weight, sex, pathological condition, diet, administration time, administration route, excretion rate, and reaction sensitivity of the patient. Generally, the dosage is 1 to 20 mg / kg / day, preferably 5 to 10 mg / kg / day, and may be administered several times a day at regular intervals, preferably 2 to 3 times a day, according to the judgment of a doctor or pharmacist.

[0048] The pharmaceutical composition of the present invention may be a topical skin preparation. The topical skin preparation is a formulation that can be applied externally to the skin. When the pharmaceutical composition of the present invention is used as a topical skin preparation, it may be applied to the scalp, specifically, to the scalp at a site where hair loss has occurred or the scalp at a site where hair growth is desired to be promoted. The topical skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The topical skin preparation may be appropriately blended with ingredients commonly used in topical skin preparations such as cosmetics and pharmaceuticals, for example, aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, UV absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or combinations thereof, as needed. The topical skin preparations may also be appropriately formulated with sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, verapamil, licorice extract, glabridin, hot water extract of Chinese quince, various herbal medicines, drugs such as tocopherol acetate, glycyrrhizinic acid, tranexamic acid and derivatives thereof or salts thereof, vitamin C, magnesium ascorbyl phosphate, ascorbyl glucoside, arbutin, kojic acid, and sugars such as glucose, fructose, and trehalose.

[0049] 3. Cosmetic composition for preventing or improving hair loss Yet another aspect of the present invention provides a cosmetic composition for preventing or improving hair loss, comprising the peptide.

[0050] The peptide contains the amino acid sequence of SEQ ID NO: 1, and the description thereof is the same as that of the peptide described in the section "Peptides having anti-hair loss or hair growth promoting activity and their uses," so the detailed description is incorporated herein by reference. Hereinafter, only the components specific to the cosmetic composition will be described.

[0051] In the present invention, the term "amelioration" refers to any action that makes the symptoms of a disease or disorder better or less severe.

[0052] The prevention or amelioration of hair loss may be achieved by removing the cause of hair loss or inhibiting the progression of hair loss, or by inhibiting hair loss or promoting hair formation to promote hair growth.

[0053] The cosmetic composition may contain a cosmetically effective amount of the peptide and a cosmetically acceptable carrier, and the cosmetically effective amount means an amount sufficient to achieve the aforementioned efficacy of preventing hair loss or promoting hair growth.

[0054] The cosmetic composition of the present invention may further contain other ingredients that can enhance the activity of the peptide, for example, within the range that does not affect the hair loss prevention or hair growth promotion activity of the peptide. For example, it may contain adjuvants commonly used in the cosmetic or dermatological fields, such as fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, moisturizing agents, essential oils, dyes, pigments, fragrances, hydrophilic or lipophilic active agents, lipid vesicles, or any other ingredients commonly used in cosmetics, and the ingredients may be contained in amounts commonly used in the cosmetic or dermatological fields.

[0055] The cosmetic composition of the present invention may be prepared in any formulation commonly used in the art, for example, a solution, suspension, emulsion, gel, lotion, essence, cream, powder, soap, shampoo, conditioner, sheet mask, surfactant-containing cleanser, cleansing foam, cleansing water, oil, liquid foundation, cream foundation, or spray.

[0056] When the dosage form is a solution or emulsion, the carrier component may be a solvent, solubilizer, or emulsifier, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, or sorbitan fatty acid esters. When the dosage form is a suspension, the carrier component may be a liquid diluent such as water, ethanol, or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters, and polyoxyethylene sorbitan esters, aluminum metahydroxide, microcrystalline cellulose, bentonite, agar, or tragacanth. When the dosage form is a cream or gel, the carrier component may be wax, paraffin, tragacanth, animal oil, starch, cellulose derivatives, silicone, bentonite, polyethylene glycol, silica, zinc oxide, or talc. When the formulation is a powder or spray, the carrier component may include a propellant such as silica, talc, aluminum hydroxyl group, lactose, calcium silicate, chlorofluorohydrocarbon, propane / butane, or dimethyl ether.When the formulation is a surfactant-containing cleanser, the carrier component may include fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, isethionate, methyl taurate, sarcosinate, fatty acid amide ether sulfate, fatty alcohol, alkylamidobetaine, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

[0057] Specifically, the cosmetic composition of the present invention may be a topical skin preparation. The topical skin preparation is a formulation that can be applied externally to the skin. When the cosmetic composition of the present invention is used as a topical skin preparation, it may be applied to the scalp, specifically, to the scalp at a site where hair loss has occurred or a site where hair growth is desired to be promoted. The topical skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The topical skin preparation may be appropriately blended, as needed, with ingredients typically used in topical skin preparations such as cosmetics and pharmaceuticals, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, UV absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or combinations thereof. The topical skin preparation may also be appropriately formulated with sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, verapamil, licorice extract, glabridin, hot water extract of Chinese quince, various herbal medicines, drugs such as tocopherol acetate, glycyrrhizinic acid, tranexamic acid and derivatives thereof or salts thereof, vitamin C, magnesium ascorbyl phosphate, ascorbyl glucoside, arbutin, kojic acid, and sugars such as glucose, fructose, and trehalose.

[0058] In another aspect of the present invention, there is provided a method for treating hair loss, comprising administering a therapeutically effective amount of the peptide to a subject in need of treatment for hair loss.

[0059] In another aspect of the present invention, there is provided a method for improving hair loss, comprising administering a cosmetically effective amount of the peptide to a subject in need of improvement of hair loss.

[0060] In another aspect of the present invention, there is provided a use of the peptide for treating alopecia.

[0061] In another aspect of the present invention, there is provided a use of the peptide in the manufacture of a medicament for treating or preventing alopecia. [Effects of the Invention]

[0062] The peptides provided by the present invention have the activity of promoting the proliferation of cells that form hair follicles or are involved in hair formation, such as dermal papilla cells, outer root sheath cells, and hair matrix cells, or promoting the activity of these cells.

[0063] Specifically, the peptide of the present invention can increase the expression level of genes or proteins related to the proliferation, differentiation, and activation of the cells, and has the activity of suppressing the expression of hair loss-related proteins induced by hormones such as DHT. Therefore, the peptide and a composition containing the same can be useful for preventing, treating, or ameliorating hair loss or promoting hair growth.

[0064] In addition, since the peptide of the present invention and compositions containing the same use a single peptide as an active ingredient, compared to hair loss improving agents using natural products that contain various ingredients and may produce unpredictable effects, the peptide and compositions containing the same have the advantage of locally inhibiting hair loss and then being easily degraded and removed by protease in the body, and have a clearer mechanism of action, which reduces the possibility of side effects.Furthermore, the composition of the present invention using a peptide as an active ingredient has the advantage of having higher biocompatibility than existing therapeutic agents because it uses a substance consisting of amino acid bonds as an active ingredient, and the peptide has the advantage of potentially showing similar activity by mimicking the active site of proteins in the human body, and is also effective in reducing problems such as decreased sexual function due to hormone regulation.

[0065] However, the effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the following description. [Brief explanation of the drawings]

[0066] [Figure 1]1 is a graph showing the results of treating HHFDPC with a peptide containing the amino acid sequence of SEQ ID NO: 1 of the present invention at various concentrations, examining whether or not cells proliferated, and comparing the results with those of a control group (Con) that received no treatment and a positive control group (MNX) that received minoxidil treatment. [Figure 2] The results show that phosphorylated AKT and ERK (p-AKT and p-ERK, respectively) were confirmed by Western blotting in HHFDPCs treated with various concentrations of a peptide containing the amino acid sequence of sequence number 1 of the present invention, a control group (Con) with no treatment, and a positive control group (MNX) treated with minoxidil. [Figure 3] This shows the results of Western blotting to confirm that β-catenin had translocated to the nucleus in HHFDPC treated with a peptide containing the amino acid sequence of sequence number 1 of the present invention at various concentrations, or in a control group (Con) that received no treatment. [Figure 4] The expression of LEF-1, c-Myc, and cyclin D1 was confirmed via RT-PCR in HHFDPCs treated with various concentrations of a peptide containing the amino acid sequence of sequence number 1 of the present invention, or in a control group (Con) that received no treatment. [Figure 5] The results show that DKK-1 protein was confirmed by Western blotting in DHT-treated HHFDPCs treated with various concentrations of a peptide containing the amino acid sequence of SEQ ID NO: 1 of the present invention, a control group (Con) with no treatment, and a positive control group (Finasteride) treated with finasteride. [Figure 6] The expression of Ha3-II, Keratin 14, and Keratin 19 was confirmed via RT-PCR in HHORSCs treated with various concentrations of a peptide containing the amino acid sequence of SEQ ID NO: 1 of the present invention, a control group (Con) with no treatment, and a positive control group treated with EGF. [Figure 7] The results show that MSX2 expression was confirmed via RT-PCR in HHGMC treated with various concentrations of a peptide containing the amino acid sequence of sequence number 1 of the present invention, a control group (Con) with no treatment, and a positive control group treated with EGF. DETAILED DESCRIPTION OF THE INVENTION

[0067] The present invention will be described in more detail below with reference to examples.

[0068] However, the following examples are provided to specifically illustrate the present invention, and the content of the present invention is not limited to the following examples.

[0069] Example [Production example] Peptide production Peptides having the amino acid sequence of SEQ ID NO: 1 listed in Table 1 below were synthesized using an automated peptide synthesizer (Milligen 9050, Millipore, USA), and the synthesized peptides were purified and separated using C18 reverse-phase high-performance liquid chromatography (HPLC) (Waters Associates, USA). The column used was an ACQUITY UPLC BEH300 C18 (2.1 mm x 100 mm, 1.7 μm, Waters Co., USA).

[0070] [Table 1] [Experimental Example 1] Confirmation of the effect of the peptide of the present invention on promoting proliferation and activity of dermal papilla cells Using the peptide of the present invention consisting of the amino acid sequence of SEQ ID NO: 1 produced by the above production example, the effects of promoting the proliferation and activity of dermal papilla cells, which are one of the cells that make up hair follicles, were confirmed, as was the inhibitory effect on hair loss-related proteins.

[0071] [1-1] Confirmation of the effect of promoting proliferation of dermal papilla cells HHFDPC (human hair follicle dermal papilla cells) were cultured and treated with the peptide of the present invention, and the absorbance was then measured to confirm whether or not the peptide has an effect of promoting the proliferation of the HHFDPC dermal papilla cells.

[0072] Specifically, 4x10 HHFDPC (Sciencell, USA) 3 Cells / well were seeded into a 96-well culture plate and cultured in complete MSC medium (mesenchymal stem cell complete media, Sciencell, USA) for 24 hours. The medium was then replaced with serum-free complete MSC medium and cultured for another 24 hours. The HHFDPCs were then treated with the peptide of SEQ ID NO: 1 prepared in the Preparation Example at concentrations of 500 nM, 5 μM, and 50 μM and cultured at 37°C for 72 hours. A positive control group was treated with minoxidil at a concentration of 1 μM, and untreated HHFDPCs served as a negative control group. Cells were then stained by adding 10 μl of 5 mg / ml MTT solution and culturing them in a CO2 incubator at 37°C for 4 hours. After removing the medium, the plates were treated with 100 μl of DMSO and shaken for 10 minutes. The number of HHFDPC cells was determined by measuring the absorbance at 540 nm using a spectrophotometer.

[0073] As a result, as can be seen in Figure 1, the absorbance was measured to be higher in the group treated with the peptide of the present invention compared to the control group, demonstrating that the proliferation of HHFDPCs was promoted in a concentration-dependent manner depending on the treated peptide, resulting in a greater increase in cell number. Based on these results, it was confirmed that the peptide of the present invention has the effect of increasing the number of dermal papilla cells, and because dermal papilla cells are cells that form hair follicles that produce hair, it was confirmed that the peptide of the present invention has the effect of promoting hair growth and improving hair loss.

[0074] [1-2] Confirmation of the effect of increasing factors related to the proliferation of dermal papilla cells In HHFDPCs treated with the peptides of the present invention, changes in related factors that affect cell proliferation or differentiation were confirmed, and it was confirmed whether the peptides of the present invention can increase these factors and promote the proliferation and differentiation of HHFDPCs.

[0075] First, the phosphorylated forms of AKT and ERK, which act as signaling molecules for the proliferation of HHFDPCs, were confirmed by Western blotting. Specifically, HHFDPCs as described in [Experimental Example 1-1] were cultured at 4×10 5 Cells / well were seeded into 6-well culture plates and cultured in complete MSC medium for 24 hours. The medium was then replaced with serum-free complete MSC medium and cultured for another 24 hours. The HHFDPCs were then treated with the peptide of SEQ ID NO: 1 prepared in the Preparation Example at concentrations of 500 nM, 5 μM, and 50 μM and cultured at 37°C for 24 hours. A positive control group was treated with minoxidil at a concentration of 1 μM, and untreated HHFDPCs served as a negative control group. Western blotting was then performed to detect phosphorylated AKT and phosphorylated ERK. After washing with PBS, the cells were treated with 100 μL of lysis buffer to obtain cell lysates. The lysates were electrophoresed on a 10% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% skim milk for 30 minutes at room temperature. To detect phosphorylated AKT (p-AKT) and phosphorylated ERK (p-ERK), anti-p-AKT and anti-p-ERK antibodies (Cell Signaling, USA), which specifically bind to p-AKT and p-ERK, respectively, were diluted 1:1000 in 3% BSA and incubated with the PVDF membrane for 16 hours at 4°C. After washing three times with 0.1% PBS-T (0.1% Tween-20 in PBS) for 15 minutes each, secondary antibodies specific for the p-AKT and p-ERK antibodies were diluted 1:2000 in 5% skim milk and incubated at room temperature for 1 hour. After washing three times with 0.1% PBS-T (0.1% Tween-20 in PBS) for 15 minutes each, p-AKT and p-ERK were detected by treatment with ECL solution (GE Healthcare, USA).

[0076] As a result, as can be seen in Figure 2, in HHFDPCs treated with the peptide of the present invention, p-AKT and p-ERK, which are signaling molecules related to proliferation, were detected, and it was shown that the phosphorylation of these molecules was increased compared to the control group that was not treated with the peptide. From these results, it was confirmed that the peptide of the present invention has the effect of activating proliferation-related signals in dermal papilla cells that make up hair follicles, promoting the proliferation of dermal papilla cells and promoting hair growth.

[0077] In addition to p-AKT and p-ERK, to confirm whether β-catenin, which is known to affect cell proliferation and differentiation, was activated, Western blotting was used to confirm whether β-catenin was translocated into the nucleus. Specifically, 4×10 HHFDPCs as described in [Experimental Example 1-1] were cultured. 5Cells / well were seeded into 6-well culture plates and cultured in complete mesenchymal stem cell media for 24 hours. The medium was then replaced with serum-free complete MSC media and cultured for another 24 hours. The HHFDPCs were then treated with the peptide of SEQ ID NO: 1 prepared in the Preparation Example at concentrations of 500 nM, 5 μM, and 50 μM and cultured at 37°C for 24 hours. A positive control group was treated with minoxidil at a concentration of 1 μM, and untreated HHFDPCs served as a negative control. After washing with PBS, nuclear proteins were isolated using a nuclear protein extraction kit (Thermo Scientific, USA). The gel was electrophoresed on a 10% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% skim milk for 30 minutes at room temperature. To detect β-catenin, anti-β-catenin (Cell Signaling, USA), which specifically binds to β-catenin, was diluted 1:1000 in 3% BSA and incubated with the PVDF membrane for 16 hours at 4°C. After three 15-minute washes with 0.1% PBS-T (0.1% Tween-20 in PBS), a secondary antibody specific for the β-catenin antibody was diluted 1:2000 in 5% skim milk and incubated for 1 hour at room temperature. After three 15-minute washes with 0.1% PBS-T (0.1% Tween-20 in PBS), β-catenin was detected by treatment with ECL solution (GE Healthcare, USA).

[0078] As a result, as can be seen in Figure 3, it was shown that β-catenin migrated and was present within the nuclei of HHFDPCs treated with the peptide of the present invention, and the migration due to β-catenin activation was increased compared to the control group not treated with the peptide, confirming that β-catenin activation was further promoted depending on the concentration of the peptide of the present invention.

[0079] Furthermore, to confirm whether β-catenin translocates to the nucleus and is activated, affecting proliferation and differentiation, we examined whether the expression of LEF-1, c-Myc, and cyclin D1, which act as subordinate factors of β-catenin activation, increased. Specifically, HHFDPC cells were cultured in the same manner as in the Western blotting experiment and treated with the peptides of the present invention at concentrations of 500 nM, 5 μM, and 50 μM, respectively. After incubation at 37°C for 24 hours, the cells were washed with PBS and treated with 300 μl of Easy Blue (Intron, Korea) to isolate RNA. The cells were then quantified using a nanodrop™ system. cDNA was synthesized using a cDNA synthesis kit (Enzynomics, Korea). PCR was performed using primers for LEF-1, c-Myc, and cyclin D1 (primer sequences are listed in Table 2 below) and PCR premix (Enzynomics, Korea). After electrophoresis on a 1.5% agarose gel, mRNAs of LEF-1, c-Myc, and cyclin D1 were detected using a band analysis system (Bio-Rad gel image system).

[0080] [Table 2] As a result, as can be seen in Figure 4, mRNA for LEF-1, c-Myc, and cyclin D1 was detected in HHFDPCs treated with the peptide of the present invention, and the amount of mRNA was increased compared to the control group without any treatment. Therefore, it was confirmed that in dermal papilla cells treated with the peptide of the present invention, the expression of LEF-1, c-Myc, and cyclin D1, which are expressed in response to the activation of β-catenin, is promoted, thereby promoting the proliferation and differentiation of dermal papilla cells and promoting hair formation.

[0081] [Experimental Example 2] Confirmation of the inhibitory effect of the peptide of the present invention on the expression of hair loss-related proteins in dermal papilla cells Dickkopf-related protein 1 (DKK-1) is a hair loss-related protein known to be highly expressed in individuals with hair loss, particularly male pattern baldness, and its expression is known to be promoted by hormones such as dihydrotestosterone (DHT). Therefore, HHFDPC cells were treated with DHT together with a peptide according to a preparation example of the present invention to induce DKK-1 protein expression, and the level of DKK-1 expression was then measured by Western blotting to confirm whether there was an inhibitory effect on DKK-1 protein expression.

[0082] Specifically, the HHFDPC as in [Experimental Example 1-1] was mixed with 4×10 5Cells / well were seeded into 6-well culture plates and cultured in complete MSC medium for 24 hours. The medium was then replaced with serum-free complete MSC medium and cultured for another 24 hours. The HHFDPCs were then treated with the peptide of SEQ ID NO: 1 prepared in the above Preparation Example at concentrations of 500 nM, 5 μM, and 50 μM, respectively, along with DHT, an inducer of DKK-1 protein expression, at a concentration of 100 nM, and cultured at 37°C for 24 hours. As a positive control, cells were treated with finasteride at a concentration of 5 μM. As a negative control, cells were treated with DHT alone to induce DKK-1 expression but without any peptide. Western blotting was then performed to detect DKK-1 protein, a protein associated with hair loss. After washing with PBS, the cells were treated with a lysis buffer to obtain cell lysates, which were then electrophoresed on a 10% SDS-PAGE gel and transferred to a PVDF membrane. The lysates were then blocked with 5% skim milk for 30 minutes at room temperature. To detect the DKK-1 protein, an anti-DKK-1 antibody (Cell Signaling, USA) that specifically binds to DKK-1 was diluted 1:1000 in 3% BSA and incubated with the PVDF membrane for 16 hours at 4°C. After washing three times with 0.1% PBS-T (0.1% Tween-20 in PBS) for 15 minutes each, a secondary antibody specific to the anti-DKK-1 antibody was diluted 1:2000 in 5% skim milk and incubated at room temperature for 1 hour. After washing three times for 15 minutes with 0.1% PBS-T (0.1% Tween-20 in PBS), the cells were treated with ECL solution (GE Healthcare, USA) to detect DKK-1 protein.

[0083] As a result, as can be seen in Figure 5, the amount of DKK-1 protein was reduced in HHFDPCs treated with the peptide of the present invention compared to the negative control group. That is, treatment of dermal papilla cells with DHT increased the expression of hair loss-related proteins (negative control group), but when treated with the peptide of the present invention, the amount of DKK-1 protein that had increased with DHT treatment in HHFDPCs decreased again, and the increased DKK-1 expression decreased again in a concentration-dependent manner depending on the peptide of the present invention, confirming that the peptide of the present invention has the effect of suppressing hair loss.

[0084] [Experimental Example 3] Confirmation of the effect of the peptide of the present invention on promoting outer root sheath cell activity Using the peptide of the present invention consisting of the amino acid sequence of SEQ ID NO: 1 produced in the above Production Example, it was confirmed whether or not it promotes the activity of outer root sheath cells, which are a type of cells that make up hair follicles.

[0085] Specifically, 4 x 10 HHORSC (human hair outer root sheath cell, Sciencell, USA) 5Cells / well were seeded into 6-well culture plates and cultured in complete mesenchymal stem cell media for 24 hours. The medium was then replaced with serum-free complete MSC media and cultured for another 24 hours. The HHORSCs were then treated with the peptide of SEQ ID NO: 1 prepared in the above Preparation Example at concentrations of 500 nM, 5 μM, and 50 μM, respectively, and cultured at 37°C for 72 hours. HHORSCs treated with EGF at a concentration of 50 nM served as a positive control, while untreated HHORSCs served as a negative control. After washing with PBS, RNA was isolated by treatment with 300 μl of Easy Blue (Intron, Korea). The RNA was then quantified using a nanodrop™ system and cDNA was synthesized using a cDNA synthesis kit (Enzynomics, Korea). PCR was performed using primers for Ha3-II, Keratin 14, and Keratin 19 (primer sequences are listed in Table 3) and PCR premix (Enzynomics, Korea). After electrophoresis on a 1.5% agarose gel, mRNAs of Ha3-II, Keratin 14, and Keratin 19 were detected using a band analysis system (Bio-Rad gel image system).

[0086] [Table 3] As a result, as can be seen in Figure 6, mRNA for Ha3-II, Keratin 14, and Keratin 19 was detected in HHORSCs treated with the peptide of the present invention, and the amount of mRNA was increased compared to the control group without any treatment. Therefore, it was confirmed that in outer root sheath cells treated with the peptide of the present invention, the expression of the above cytokines increased as the cell activity increased, and it was confirmed that the peptide of the present invention has the effect of increasing the activity of outer root sheath cells, which form hair follicles and help form hair.

[0087] [Experimental Example 4] Confirmation of the effect of the peptide of the present invention on promoting hair matrix cell activity Using the peptide of the present invention consisting of the amino acid sequence of SEQ ID NO: 1 prepared in the above Preparation Example, it was confirmed whether or not it promotes the expression of a transcription factor associated with the activity of hair matrix cells, which are a type of cells that form hair.

[0088] Specifically, 4 x 10 HHGMC (human hair germinal matrix cells, Sciencell, USA) 5 Cells / well were seeded into 6-well culture plates and cultured in complete MSC medium (mesenchymal stem cell complete media) for 24 hours. The medium was then replaced with serum-free complete MSC medium and cultured for another 24 hours. The HHGMCs were then treated with the peptide of SEQ ID NO: 1 prepared in the Preparation Example at concentrations of 500 nM, 5 μM, and 50 μM and cultured at 37°C for 72 hours. A positive control group was treated with EGF at a concentration of 50 nM, and untreated HHGMCs were used as a negative control group. After washing with PBS, RNA was isolated by treatment with 300 μl of Easy Blue (Intron, Korea). The RNA was then quantified using a nanodrop™ system and cDNA was synthesized using a cDNA synthesis kit (Enzynomics, Korea). PCR was performed using primers for MSX2 (primer sequences are listed in Table 4 below) and PCR premix (Enzynomics, Korea). After electrophoresis on a 1.5% agarose gel, MSX2 mRNA was detected using a band analysis system (Bio-Rad gel image system).

[0089] [Table 4] As a result, as can be seen in Figure 7, MSX2 mRNA was detected in HHGMC treated with the peptide of the present invention, and the amount of mRNA was increased compared to the control group without any treatment. Therefore, in hair matrix cells treated with the peptide of the present invention, the expression of the transcription factor MSX2 increased, and the transcription and resulting activity of genes related to cell activity increased, confirming that the peptide of the present invention has the effect of increasing the activity of hair matrix cells, promoting hair formation, and preventing and improving hair loss.

[0090] Although the present invention has been described in detail above only with reference to the embodiments described, it will be apparent to those skilled in the art that various modifications and variations are possible within the scope of the technical concept of the present invention, and it is natural that such modifications and variations fall within the scope of the appended claims.

Claims

1. A peptide consisting of the amino acid sequence of SEQ ID NO: 1, which has hair loss prevention or hair growth promotion activity.

2. The peptide according to claim 1, wherein the peptide promotes proliferation or activity of cells involved in hair formation.

3. A composition for preventing hair loss or promoting hair growth, comprising the peptide of claim 1.

4. 4. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the peptide is contained in the composition at a concentration of 0.01 μM to 100 μM.

5. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition promotes the activity of one or more cells selected from the group consisting of hair papilla cells, outer root sheath cells, and hair matrix cells, thereby inducing hair formation.

6. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition induces phosphorylation of one or more selected from the group consisting of AKT and ERK in hair papilla cells.

7. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition induces activation of β-catenin in hair papilla cells.

8. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition promotes the expression of one or more selected from the group consisting of LEF-1, c-Myc, and cyclin D1 in hair papilla cells.

9. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition suppresses the expression of DKK-1 protein in hair papilla cells.

10. 4. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition promotes the expression of one or more selected from the group consisting of Ha3-II, Keratin 14, and Keratin 19 in outer root sheath cells.

11. The composition for preventing hair loss or promoting hair growth according to claim 3, wherein the composition promotes the expression of MSX2 in hair matrix cells.

12. A pharmaceutical composition for preventing or treating hair loss, comprising the peptide of claim 1.

13. The pharmaceutical composition for preventing or treating hair loss according to claim 12, wherein the pharmaceutical composition is an external preparation for skin.

14. A cosmetic composition for preventing or improving hair loss, comprising the peptide according to claim 1.

15. The cosmetic composition for preventing or ameliorating hair loss according to claim 14, wherein the cosmetic composition is a topical agent for skin.

16. 15. The cosmetic composition for preventing or ameliorating hair loss according to claim 14, wherein the cosmetic composition has any one dosage form selected from the group consisting of a solution, a suspension, an emulsion, a gel, a lotion, an essence, a cream, a powder, a soap, a shampoo, a conditioner, a sheet mask, a surfactant-containing cleanser, a cleansing foam, a cleansing water, an oil, a liquid foundation, a cream foundation, and a spray.

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