Method for producing a composition containing phytic acid from rice bran

Abstract

The present application relates to a method for producing a composition containing phytic acid from rice bran. According to the present application, a degreasing step of rice bran is not required, and a composition containing phytic acid can be efficiently produced from rice bran without using strong acids such as hydrochloric acid or sulfuric acid. The composition containing phytic acid produced according to the present application has a low concentration of inorganic ions, high antioxidant activity, and antibacterial activity against fermentation microorganisms, and can be used to control fermentation microorganisms.

Description

[Technical Field]

[0001] This application relates to a method for producing a composition containing phytic acid from rice bran. [Background technology]

[0002] Rice bran is the fine bran separated from rice plants after the husks have been removed, during the process of polishing brown rice into white rice. Rice bran may contain the pericarp, seed coat, aleurone layer, and part of the embryo, and is in the form of a pulverized material.

[0003] Conventional techniques for separating phytic acid from rice bran involve treating the rice bran with an acid such as hydrochloric acid or sulfuric acid to obtain an extract, and then neutralizing the extract by adding sodium hydroxide to obtain a precipitate containing phytic acid (Korean Registered Patent No. 10-0471558).

[0004] Thus, in the method of extracting phytic acid from rice bran using acid, a degreasing process is necessary because the raw material, rice bran, contains a large amount of oil. If phytic acid is produced without going through the degreasing process, it acts as an impurity in the refining process, making it difficult to purify to a high purity.

[0005] Furthermore, the precipitate containing phytic acid obtained by neutralizing rice bran after acid extraction begins to contain a large amount of protein. This is because, during the neutralization process, a large amount of protein interferes with the phytic acid precipitate, and the oil present in the rice bran also reacts with the protein and precipitates. When the phytic acid precipitate contains proteins that are difficult to separate, it becomes difficult to remove the oil and protein in the ion exchange process, which is a purification step. The remaining proteins and oils thus degrade the quality of the final phytic acid and worsen its shelf life.

[0006] Due to the drawbacks of such prior art, it is necessary to develop a new method for efficiently separating phytic acid from rice bran using enzymes without using hydrochloric acid or sulfuric acid.

Prior Art Documents

Patent Documents

[0007]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0008] The present inventor has made research efforts to develop a method for producing a composition containing phytic acid from rice bran without a degreasing process of rice bran and a strong acid treatment process such as hydrochloric acid or sulfuric acid. As a result, after treating rice bran with an enzyme having polysaccharide-degrading activity, proteolytic activity, or lipolytic activity and then performing a filtration, neutralization, and ion exchange resin treatment process on the enzyme-treated product, it was experimentally confirmed that a phytic acid-containing composition having a low content of inorganic ions and high antioxidant and antibacterial activities can be produced without using a process of treating with an acid such as hydrochloric acid or sulfuric acid, and thus the present invention was completed.

[0009] Therefore, an object of the present application is to provide a method for producing a composition containing phytic acid from rice bran without a degreasing process and an acid treatment process.

[0010] Another object of the present application is to provide a phytic acid-containing composition having a low concentration of inorganic ions and high antioxidant activity.

[0011] Still another object of the present application is to provide a phytic acid-containing composition having antibacterial activity against fermenting microorganisms.

Means for Solving the Problems

[0012] In order to achieve the above object, One aspect of the present application provides a method for producing a phytic acid-containing composition from rice bran, comprising the following steps: (a) Treating rice bran with an enzyme to produce an enzyme extract of rice bran; (b) Neutralizing the rice bran enzyme extract to produce a precipitate; (c) Redissolving the precipitate to produce a redissolved product; and (d) Contacting the redissolved product with an ion exchange resin.

[0013] Another aspect of the present application provides a phytic acid-containing composition having a phytic acid content of 35% by weight or more based on the total composition, and the total concentration of ions of Na + , NH 4+ , K + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- is more than 0 ppm and 400 ppm or less.

[0014] Still another aspect of the present application is an antibacterial composition containing a phytic acid-containing composition as an active ingredient, (i) The phytic acid-containing composition has a phytic content of 35% by weight or more based on the phytic acid-containing composition, and the total concentration of ions of Na + , NH 4+ , K + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- is more than 0 ppm and 400 ppm or less, and (ii) It has antibacterial activity against Bacillus subtilis.

[0015] The present application will be described in detail below.

[0016] In one aspect of the present application, a method for producing a phytic acid-containing composition from rice bran, comprising the following steps (a) to (d), is provided.

[0017] Stage (a): A stage in which rice bran is treated with enzymes to produce an enzyme extract from rice bran.

[0018] In this application, an enzyme extract of rice bran is obtained by treating rice bran with an enzyme. In this application, the term "enzyme extract of rice bran" means an enzyme-treated product of rice bran obtained by treating rice bran with an enzyme, and in this specification, "enzyme extract of rice bran" is used with the same meaning as "enzyme-treated product of rice bran".

[0019] In this application, the enzyme used to treat rice bran may be an enzyme having one or more activities of polysaccharide degradation activity, protein degradation activity, and lipolysis activity.

[0020] Specifically, the enzyme may be an enzyme having polysaccharide-degrading activity, and the polysaccharide-degrading activity may be xylan-degrading activity. More specifically, the enzyme may have polysaccharide-degrading activity, protein-degrading activity, and / or lipolytic activity, and the enzyme may be a multi-enzyme complex having two or more of the above activities.

[0021] In one embodiment, the enzyme used to treat the rice bran in step (a) may be one or more, more specifically two or more, or three or more, enzymes selected from the group consisting of beta-glucanase, endo-1,3(4)-beta-glucanase 9 (beta-glucanase(endo-1,3(4)-), cellulase, hemicellulase, xylanase, beta-xylanase, endo-1-4-beta-xylanase, alpha-amylase, alpha-amylase, pullulanase, lipase, and protease activity.

[0022] Specifically, the enzyme of this application may have xylan-degrading activity, more specifically xylanase, beta-xylanase, or endo-1-4-beta-xylanase activity, and may further have one or more activities selected from the group consisting of beta-glucanase, endo-1,3(4)-beta-glucanase, cellulase, hemicellulase, alpha-amylase, alpha-amylase, pullulanase, lipase, and protease activity.

[0023] In one embodiment, the enzyme used in this application may be a commercially available enzyme, and in non-restrictive examples, one or more enzymes selected from the group consisting of the following enzymes may be used: pentopan 500BG, Celluclast 1.5L, Ultraflo Max, Cellic® CTec2, Shearzyme plus, Viscoflow MG, Viscoferm, Ondea Pro, Ceremix 6X MG, ​​and Viscozyme L.

[0024] In a more specific example, the enzyme used in this application may be a commercially available enzyme, and may be one or more enzymes selected from the group consisting of the following enzymes as non-limiting examples: pentopan 500BG, Ceremix 6X MG, ​​Ultraflo Max, Ondea Pro, Shearzyme plus, and Viscozyme L.

[0025] In one embodiment, the enzymatic treatment of rice bran may be carried out under conditions in which the enzyme is brought into contact with the rice bran and reacts with the rice bran, for example, by preparing an enzymatic treatment reaction solution containing rice bran and the enzyme.

[0026] The rice bran content in the enzyme-treated reaction solution may be, for example, 1-30% by weight, 1-28% by weight, 1-27% by weight, 1-26% by weight, 2-28% by weight, 2-27% by weight, 2-26% by weight, 3-28% by weight, 3-27% by weight, 3-26% by weight, 5-25% by weight, 6-25% by weight, 7-25% by weight, 8-22% by weight, 8-20% by weight, 8-18% by weight, 8-16% by weight, 8-14% by weight, or 8-12% by weight, but is not limited thereto.

[0027] The enzyme volume in the enzyme-treated reaction solution may be 0.1-1% by weight. Specifically, the enzyme volume may be 0.1-0.9% by weight, 0.1-0.8% by weight, 0.1-0.7% by weight, 0.1-0.6% by weight, 0.2-0.9% by weight, 0.3-0.9% by weight, 0.4-0.9% by weight, 0.2-0.8% by weight, 0.2-0.7% by weight, 0.2-0.6% by weight, 0.3-0.8% by weight, 0.3-0.7% by weight, 0.3-0.6% by weight, 0.4-0.8% by weight, 0.4-0.7% by weight, or 0.4-0.6% by weight.

[0028] The pH of the enzyme-treated reaction solution may be set considering the optimal pH of the enzyme used, for example, 4.3-5.5, 4.3-5.4, 4.3-5.3, 4.4-5.5, 4.4-5.4, 4.4-5.3, 4.5-5.5, 4.5-5.4, 4.5-5.3, 4.6-5.5, 4.6-5.4, 4.6-5.3, 4.7-5.5, 4.7-5.4, 4.7-5.3, 4.7-5.2, 4.7-5.1, or 4.7-5.0.

[0029] The temperature during the enzyme treatment reaction may be set considering the optimal reaction temperature of the enzyme used, for example, 35-55°C, 36-54°C, 37-53°C, 38-52°C, or 39-52°C.

[0030] The reaction time during the enzyme treatment reaction may be 0.1-16 hours, 0.5-16 hours, 1-16 hours, 1-15 hours, 1-14 hours, 1-13 hours, 1-12 hours, 1-11 hours, 1-10 hours, 1-9 hours, 1-8 hours, 1-7 hours, 1-6 hours, 1-5 hours, 0.1-4 hours, 0.5-4 hours, 1-4 hours, 1.2-3.8 hours, 1.4-3.6 hours, 1.6-3.4 hours, 1.8-3 hours, 1.8-2.8 hours, 1.8-2.6 hours, or 1.8-2.4 hours.

[0031] In one embodiment, the present application may further include a step of filtering the rice bran enzyme extract after step (a) and before step (b).

[0032] The filtration may be carried out by passing the enzyme extract through a sieve having an appropriate mesh range. The mesh range may be 10-500 mesh, 10-400 mesh, 10-300 mesh, 10-200 mesh, 10-100 mesh, 20-500 mesh, 50-400 mesh, 70-300 mesh, 80-200 mesh, 90-150 mesh, 90-120 mesh, or 100 mesh.

[0033] In other embodiments, a filtration aid may be used during filtration. As the filtration aid, diatomaceous earth or acid clay may be used, and diatomaceous earth is preferred.

[0034] In other embodiments, the filtration may be carried out using filter paper, and may further include a filtration process using, for example, Whatman filter paper.

[0035] Stage (b): Stage in which the rice bran enzyme extract is neutralized to produce a precipitate.

[0036] In this application, the rice bran enzyme extract or filtered rice bran enzyme extract is neutralized to obtain a precipitate.

[0037] In one example, neutralization of the rice bran enzyme extract may be performed by adjusting the pH of the rice bran enzyme extract to a range of 7 to 7.5.

[0038] In other embodiments, the neutralization of the rice bran enzyme extract by pH adjustment may be carried out by adding an acidity adjusting substance to the rice bran enzyme extract, and the acidity adjusting substance may be, for example, sodium bicarbonate (NaHCO3), monosodium phosphate (NaH2PO4), or disodium phosphate (Na2HPO4).

[0039] In other embodiments, the resulting precipitate may be dried before use in the next redissolution step.

[0040] Stage (c): A stage in which the precipitate is redissolved to produce a redissolved product.

[0041] The precipitate obtained in step (b) above is redissolved.

[0042] In one embodiment, the redissolution of the precipitate may be carried out by adding an acidic substance, which may be, but is not limited to, one or more selected from the group consisting of, for example, citric acid, lactic acid, malic acid, and tartaric acid.

[0043] In one embodiment, the acidic solution containing the dissolved acidic substance may be added to the precipitate to redissolve it. The concentration of the acidic substance in the acidic solution is not particularly limited and may be used at a concentration in the range of 0.5-3%, for example.

[0044] In one specific embodiment, the redissolved product may be a liquid solution, and the redissolved liquid solution may be further treated with activated carbon to remove pigments or foreign matter.

[0045] Step (d): Step of bringing the redissolved material into contact with the ion exchange resin.

[0046] The liquid redissolved product obtained in step (c) above is brought into contact with an ion exchange resin to remove ions from the redissolved product solution.

[0047] In one embodiment, the ion exchange resin may be a cation exchange resin, an anion exchange resin, or a combination of all of these.

[0048] In one embodiment, the cation exchange resin of this application may be a sulfonic acid type strongly acidic cation exchange resin or a weakly acidic cation exchange resin with a carboxylic acid group as the exchange group.

[0049] In one embodiment, the anion exchange resin of this application may be a strongly basic anion exchange resin having a quaternary ammonium group as a functional group, or a weakly basic anion exchange resin having a primary-tertiary ammonium group as a functional group.

[0050] In one embodiment, the composition produced by the method for producing a composition containing phytic acid from rice bran including the above steps (a) to (d) contains phytic acid at a rate of 35% by weight or more of the total composition, and Na + NH 4+ , K + Ca 2+ Mg 2+ Cl - , PO4 3- , and SO4 2- The composition may contain phytic acid, wherein the total concentration of the ions is greater than 0 ppm and less than or equal to 400 ppm.

[0051] Specifically, in the composition containing phytic acid, the phytic acid content may be 35-60% by weight of the total composition, more specifically, 35-58% by weight, 35-57% by weight, 35-56% by weight, 35-55% by weight, 35-54% by weight, 35-53% by weight, 35-52% by weight, 38-58% by weight, 38-57% by weight, 38-56% by weight, 38-55% by weight, 38-54% by weight, 38-53% by weight, and 38-52% by weight of the total composition. It may be %, 41-58% by weight, 41-57% by weight, 41-56% by weight, 41-55% by weight, 41-54% by weight, 41-53% by weight, 41-52% by weight, 44-58% by weight, 44-57% by weight, 44-56% by weight, 44-55% by weight, 44-54% by weight, 44-53% by weight, 44-52% by weight, 47-58% by weight, 47-57% by weight, 47-56% by weight, 47-55% by weight, 47-54% by weight, 47-53% by weight, or 47-52% by weight.

[0052] In the composition containing the phytic acid, Na + NH 4+ , K + Ca 2+ Mg 2+ Cl - , PO4 3- , and SO4 2- The total concentration of the ions may be greater than 0 ppm and less than or equal to 400 ppm, specifically 10-400 ppm, 10-390 ppm, 10-380 ppm, 10-370 ppm, 10-360 ppm, 10-350 ppm, 10-345 ppm, 10-344 ppm, 30-400 ppm, 30-390 ppm, 30-380 ppm, 30-370 ppm, 30-360 ppm, 30-350 ppm, 30-345 ppm, 30-344 ppm, 50-400 ppm, 50-390 ppm, 50-380 ppm, 50-370 ppm, 50-360 ppm, 50-350 ppm, 50-345 ppm, or 50-344 ppm.

[0053] In other aspects of this application, the phytic acid content is 35% by weight or more of the total composition, and Na + NH 4+ , K + Ca2+ Mg 2+ Cl - , PO4 3- , and SO4 2- The present invention provides a composition containing phytic acid in which the total concentration of ions is greater than 0 ppm and less than or equal to 400 ppm.

[0054] In one embodiment, the composition containing phytic acid may be produced from an enzyme-treated product of rice bran.

[0055] In one embodiment, in the phytic acid-containing composition of this application, the phytic acid content may be 35-60% by weight of the total composition, more specifically, 35-58% by weight, 35-57% by weight, 35-56% by weight, 35-55% by weight, 35-54% by weight, 35-53% by weight, 35-52% by weight, 38-58% by weight, 38-57% by weight, 38-56% by weight, 38-55% by weight, 38-54% by weight, 38-53% by weight, and 38-52% by weight of the total composition. It may be 41-58% by weight, 41-57% by weight, 41-56% by weight, 41-55% by weight, 41-54% by weight, 41-53% by weight, 41-52% by weight, 44-58% by weight, 44-57% by weight, 44-56% by weight, 44-55% by weight, 44-54% by weight, 44-53% by weight, 44-52% by weight, 47-58% by weight, 47-57% by weight, 47-56% by weight, 47-55% by weight, 47-54% by weight, 47-53% by weight, or 47-52% by weight.

[0056] In one example, in the composition containing phytic acid, Na + NH 4+ , K + Ca 2+ Mg 2+ Cl - , PO4 3- , and SO4 2-The total concentration of the ions may be greater than 0 ppm and less than or equal to 400 ppm, specifically 10-400 ppm, 10-390 ppm, 10-380 ppm, 10-370 ppm, 10-360 ppm, 10-350 ppm, 10-345 ppm, 10-344 ppm, 30-400 ppm, 30-390 ppm, 30-380 ppm, 30-370 ppm, 30-360 ppm, 30-350 ppm, 30-345 ppm, 30-344 ppm, 50-400 ppm, 50-390 ppm, 50-380 ppm, 50-370 ppm, 50-360 ppm, 50-350 ppm, 50-345 ppm, or 50-344 ppm.

[0057] The phytic acid-containing composition of this application may be used as an antioxidant composition.

[0058] The phytic acid-containing composition of this application has the characteristic of having a low degree of browning.

[0059] The aforementioned low degree of browning means that the phytic acid-containing composition has excellent properties for maintaining transparency during storage, and when such a composition is used as a food additive, it can minimize undesirable color changes.

[0060] In one example, the degree of browning can be evaluated by measuring the absorbance of the composition containing phytic acid.

[0061] In one embodiment, the degree of browning of the composition containing phytic acid may be expressed as the absorbance of the composition measured at 420 nm using a spectrophotometer.

[0062] In one specific example, the composition containing phytic acid may have an absorbance value of 0.035-0.15 measured at 420 nm using a spectrophotometer, more specifically, 0.035-0.15, 0.037-0.15, 0.04-0.15, 0.04-0.14, 0.04-0.13, 0.04-0.12, 0.04-0.11, 0.045-0.15, 0.045-0.14, 0.045-0.13, 0.045-0.12, or 0.045-0.11. In this case, the absorbance measurement value may be the value measured immediately after the composition containing phytic acid is manufactured.

[0063] In other specific examples, the composition containing phytic acid may have an absorbance value of 0.09-0.19 measured at 420 nm using a spectrophotometer on day 0 of the storage period, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated so that its soluble solids content is 40-41 Brix.

[0064] In other specific examples, the composition containing phytic acid may have an absorbance value of 0.09-0.19 measured at 420 nm using a spectrophotometer after being stored at 10°C for 15 days, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated so that its soluble solids content is 40-41 Brix.

[0065] In other specific examples, the composition containing phytic acid may have an absorbance value of 0.09-0.19 measured at 420 nm using a spectrophotometer after being stored at 10°C for 30 days, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated so that its soluble solids content is 40-41 Brix.

[0066] In other specific examples, the composition containing phytic acid may have an absorbance value of 0.09-0.19 measured at 420 nm using a spectrophotometer after being stored at 10°C for 45 days, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated so that its soluble solids content is 40-41 Brix.

[0067] In other specific examples, the composition containing phytic acid may have an absorbance value of 0.09-0.19 measured at 420 nm using a spectrophotometer after being stored at 10°C for 60 days, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.14. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated so that its soluble solids content is 40-41 Brix.

[0068] In other specific examples, the composition containing phytic acid may have an absorbance value of 0.09-0.19 measured at 420 nm using a spectrophotometer after being stored at 10°C for 75 days, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, 0.11-0.137, or 0.12-0.137. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated so that its soluble solids content is 40-41 Brix.

[0069] In other specific examples, the composition containing phytic acid may be stored at a temperature of 10°C for 0 to 75 days, and the change in absorbance measured at 420 nm on day 0 and day 75 using a spectrophotometer may be 0.015-0.008, 0.014-0.008, 0.013-0.008, 0.012-0.008, 0.011-0.008, 0.01-0.008, 0.0095-0.008, or 0.0095-0.0085. In this case, the change in absorbance is the value obtained by subtracting the absorbance measured on day 0 from the absorbance measured on day 75, and the phytic acid-containing composition whose absorbance is measured may be concentrated so that the soluble solids content is 40-41 Brix.

[0070] In a more specific example, the absorbance may be a value measured using a spectrophotometer U-2900 (HITACHI, Co., Japan).

[0071] In other embodiments, the composition containing phytic acid may be a composition produced by a method for producing a composition containing phytic acid from rice bran, including steps (a) to (d) described above.

[0072] In the phytic acid-containing composition of this application, the description of the method for producing the phytic acid-containing composition from rice bran will be based on the content of one aspect of this application already described, and will not be repeated.

[0073] In other aspects of this application, as an antimicrobial composition containing a composition containing phytic acid as an active ingredient, (i) the composition containing phytic acid has a phytic acid content of 35% by weight or more relative to the phytic acid-containing composition, and Na + NH 4+ , K + Ca 2+ Mg 2+ Cl - , PO4 3- , and SO4 2- The present invention provides an antimicrobial composition having (ii) antimicrobial activity against Bacillus subtilis, wherein the total concentration of the ions is greater than 0 ppm and less than or equal to 400 ppm.

[0074] In one embodiment, the antimicrobial composition containing phytic acid may be manufactured from an enzyme-treated rice bran product.

[0075] In one embodiment, the antibacterial composition containing phytic acid may be a composition produced by a method for producing a composition containing phytic acid from rice bran, including steps (a) to (d) described above.

[0076] In one embodiment, the composition containing phytic acid, which is the active ingredient of the antibacterial composition, is the same as the composition containing phytic acid described in the other aspect of this application above. Therefore, the details concerning this composition will be explained by referring to the description of the composition above and will not be repeated.

[0077] In this application, the method for producing a composition containing phytic acid from rice bran in the antibacterial composition described herein will be based on the content of one aspect of this application already described, and will not be described redundantly.

[0078] The phytic acid-containing composition of this application has antioxidant activity, antibacterial activity against fermenting microorganisms, and particularly antibacterial activity against Bacillus subtilis, and may therefore be used as a food additive in a variety of foods where such activity is required.

[0079] The phytic acid-containing compositions of this application may be applied without limitation to foods, feeds, household goods, industrial products, etc. Specific examples of foods or feeds include, but are not limited to, processed grain products, vegetables, fruits, dried or cut vegetable products, fruit juices, vegetable juices, mixed vegetable and fruit juices, chips, noodles, processed livestock products, processed seafood products, processed dairy products, fermented dairy products, microbially fermented foods, confectionery and bread products, seasonings, processed fish / meat products, acidic beverages, processed foods, convenient foods, licorice products, herbs, insect feeds, livestock feeds, pet feeds, etc.

[0080] The aforementioned processed fish / meat products refer to ham, sausages, bacon, dried meat products, seasoned meat products, packaged meat, ground meat products, kalbi products, meat extract products, edible beef fat, edible pork fat, chunks of fish meat, etc., which are processed using meat or fish as raw materials.

[0081] The aforementioned "meat" may include, but is not limited to, meats commonly used in dietary customs such as beef, pork, sheep, goats, rabbits, chickens, turkeys, ducks, pheasants, and quail, as well as edible organs and by-products. The form of processed meat products includes, but is not limited to, sterilized meat products, hams, pressed hams, mixed pressed hams, sausages, mixed sausages, dried sausages (dried mixed sausages), semi-dried sausages (semi-dried mixed sausages), heated and frozen sausages, bacons, dried preserved meats, seasoned meats, ground meat products, kalbi products, packaged meats, and other processed meat products.

[0082] When the phytic acid-containing composition of this application is used in a manner suitable for the intended purpose, it may be formulated in various forms such as liquid, solid, or powder in a convenient and suitable manner to suit the intended purpose.

[0083] The composition of this application may be incorporated together with the raw materials during the manufacturing process, and can be applied evenly to the food by immersing it in the composition of this application, stirring it after immersion, spraying the composition, or directly mixing it. [Effects of the Invention]

[0084] According to this application, a phytic acid-containing composition can be efficiently produced from rice bran without the need for a degreasing process and without using strong acids such as hydrochloric acid or sulfuric acid.

[0085] The phytic acid-containing composition produced by this application has a low concentration of inorganic ions, high antioxidant activity, and antibacterial activity against fermenting microorganisms, and can therefore be used to control fermenting microorganisms.

[0086] However, the effects of this application are not limited to those mentioned above, and any other effects not mentioned can be clearly understood by those skilled in the art from the following description. [Brief explanation of the drawing]

[0087] [Figure 1] This is a comparative photograph of the phytic acid-containing solution of this application in manufacturing example 2, and competitor products 1 (sulfuric acid treatment method) and 2 (hydrochloric acid treatment method). [Figure 2] This shows the results of measuring the antibacterial effect of the rice bran extract of this application against fermenting microorganisms using the disk diffusion method. [Modes for carrying out the invention]

[0088] The present application will be described in detail below with reference to examples. However, the following examples are for illustrative purposes only and do not limit the content of the present application. [Examples]

[0089] [Manufacturing Example 1] Production of Rice Bran Enzyme Extract

[0090] 1. Enzyme treatment of rice bran The rice bran used for the experiment was purchased after being refrigerated following the milling process, and then frozen to maintain its quality before being used as experimental material.

[0091] Enzyme-treated rice bran extracts were prepared by treating rice bran with enzymes possessing beta-glucanase, cellulase, xylanase, beta-xylanase, amylase, alpha-amylase, pullulanase, and protease activity (Novozyme A / S). The enzymes used in the experiment are listed in Table 1 below.

[0092] [Table 1]

[0093] To treat rice bran with enzymes, an enzyme treatment reaction solution for rice bran was prepared containing enzymes, rice bran, and purified water. The pH range and temperature of the enzyme were set considering the enzyme activity pH range and activity temperature provided by the manufacturer (Novozyme A / S). The pH of the enzyme treatment reaction solution was adjusted by adding 0.3% citric acid, and based on the total enzyme treatment reaction solution, 10% by weight of rice bran and 0.5% by weight of enzyme were used, with a fixed treatment time of 13-14 hours. The reaction conditions for each enzyme treatment reaction used are described in Table 2 below.

[0094] [Table 2]

[0095] 2. Solid content measurement The soluble solids content of rice bran enzyme-treated extract (hereinafter also referred to as "rice bran enzyme extract" or "rice bran extract"), obtained by treating rice bran with enzymes, was measured using an ATAGO refractometer RX-5000α. The solids content of the rice bran extracts obtained using the enzymes used is shown in Table 3 below.

[0096] [Table 3]

[0097] Among the characteristics of the enzymes, those treated with xylanases that decompose xylan and exhibit high enzyme titers showed higher solid content. The solid content was even higher in the group treated with enzymes possessing combined activity compared to those treated with single-activity enzymes.

[0098] 3. Measurement of total polyphenol content and DPPH radical scavenging activity The total polyphenol content was measured using the Folin-Ciocalteu method. 0.1 ml of a sample diluted to a specific concentration was mixed with 0.05 ml of 50% Folin-Ciocalteu reagent in a microtube and reacted at room temperature for 3 minutes. After the reaction, 0.15 ml of 2% Na2CO3 solution was added and the mixture was reacted at room temperature for 30 minutes. The content was then measured at 700 nm absorbance using a microplate reader (M2, Molecular Device, Canada). Standard calibration curves were created using gallic acid at concentrations of 50, 60, 70, 80, and 100 mg / L as the standard stock.

[0099] The DPPH radical scavenging activity was measured using the Blois method. A 4 mM DPPH (DPPH(1,1-diphenyl-2-picrylhydrazyl, Sigma Chemical Co.)) ethanol solution was prepared and its absorbance was adjusted to 1.000 ± 0.1 before use. Specifically, 0.2 mL of each sample was added to a test tube, mixed with 2.8 mL of DPPH solution, reacted for 10 minutes, and then measured at 517 nm using a Microplate reader (M2, Molecular Device, Canada). The DPPH radical scavenging activity was calculated from the following formula. DPPH radical scavenging activity(%)=[1-(A / B)]X100 (A: Absorbance of the group with added sample, B: Absorbance of the group without added sample)

[0100] The results of the measurement of the total polyphenol content and DPPH radical scavenging activity of the rice bran extract are shown in Table 4 below.

[0101] [Table 4]

[0102] It appears that the enzymes broke down cellulose, extracting many functional components present within the cell wall. Polyphenols were also extracted among these components, and the high total polyphenol content was confirmed. The enzyme with the highest polyphenol content was ceremix 6x MG, which also showed a high Ultraflo Max. Furthermore, the DPPH radical scavenging activity, which indicates antioxidant effects, was high at 94.14-90.46%, indicating it was not proportional to the total polyphenol content.

[0103] 4. Analysis of phenolic compounds To identify the polyphenol components in the rice bran enzyme extract, phenolic compounds were analyzed. The phenolic compound analysis was performed using HPLC (Agilent Technologies, 1260 infinity II, USA). 20 μL of rice bran extract was used as the sample. The column was an HPLC column (Eclipse XDB-C18, 4.6 x 250 mm, 5 μm.), and the detector was a DAD (Agilent Technologies, 1260 infinity II USA) at a wavelength of 280 nm. The mobile phase consisted of Methanol (A) and 50 mM sodium hydrogen phosphate (Na2HPO4 / pH2.5, Phosphoric acid) (B) in a 30:70 ratio. The mobile phase flow rate was set to 1.0 ml / min, and the column temperature to 40°C for 30 minutes. Standard substances were prepared at concentrations of 1, 3, 5, 6, 8, and 10 mg / L to create standard calibration curves. The results of the phenolic compound analysis are shown in Table 5 below.

[0104] [Table 5]

[0105] 5. Measurement of phytic acid content The phytic acid content in rice bran extract was measured. The indicator used for phytic acid content measurement was prepared by mixing 300 mg sulfosalicylic acid and 30 mg ferric chloride hexahydrate (wade reagent) in 100 mL of distilled water and storing it at 2-4°C. Specifically, the sample was diluted, and 3 mL of the diluent and 1 mL of the wade reagent were mixed in a 15 mL conical tube. The mixture was then centrifuged at 3000 rpm, 10°C, and 10 minutes, and the absorbance was measured at 500 nm. For the standard substance, phytic acid sodium salt hydrate obtained from rice was used to prepare a 100 mg / L standard stock, and then standard calibration curves were created at 0, 20, 40, 60, 80, and 100 mg / L. The results of the phytic acid content measurement of the rice bran extract using the treated enzyme are shown in Table 6 below.

[0106] [Table 6]

[0107] Rice bran contains phytic acid and magnesium 2+ , K + Ca 2+ It is known to exist in the form of salt mixtures bound to ions such as . Enzymatically treated rice bran extract showed that phytic acid was present in the form of crude phytic salts at a content of 0.24-0.97% by weight. This value corresponds to a phytic acid content of 2.14-8.76% by weight when converted to 100g of enzyme-treated raw rice bran. [Examples]

[0108] [Manufacturing Example 2] Production of phytic acid-containing solution

[0109] A solution containing phytic acid was prepared using the enzyme extracted with a high phytic acid content in the aforementioned Production Example 1, and the enzyme confirmed to have a high antioxidant effect in its extract. After treating rice bran with enzymes, a phytic acid-containing solution was prepared through filtration, neutralization, precipitation, redissolution, purification, and concentration processes.

[0110] 1. Enzyme treatment The enzymatic treatment was carried out under the conditions shown in Table 7 below. After treating with enzymes for 1, 2, and 4 hours, the samples were inactivated at 90°C for 10 minutes.

[0111] [Table 7]

[0112] 2. Measurement of phytic acid content by enzyme treatment time The phytic acid content was measured in enzyme extracts obtained by enzymatic treatment of rice bran, depending on the enzyme treatment time (extraction time). The phytic acid content was measured using the same method as described in Production Example 1, and the measurement results for phytic acid content are shown in Table 8 below.

[0113] [Table 8]

[0114] The results of examining the change in phytic acid content with respect to extraction time showed that the content was low in the initial stages of extraction and increased as the extraction time increased. Most enzymes extracted phytic acid at a high level of 0.618-0.726% after 2 hours of extraction, but Ceremix 6X MG extracted it at a low level of 0.397%, showing a decreasing trend. At 4 hours of extraction, a decrease in phytic acid content was observed in all enzyme-treated groups. Therefore, it was confirmed that when extracting rice bran extract using enzymes, the extraction efficiency actually decreases with longer extraction times.

[0115] 3. Separation of crude phytate solids and removal of metal ions Rice bran enzyme extract, obtained by enzymatic treatment of rice bran, was subjected to primary filtration using a 100-mesh standard. The filtered extract was subjected to secondary filtration using a Buchner funnel lined with Whatman No. 2 filter paper, with 5% by weight of diatomaceous earth as a filtration aid relative to the total weight of the extract. The secondary filtered extract was neutralized to a pH in the range of 7.00-7.50 using NaHCO3. The neutralized extract was allowed to stand for 12 hours to induce precipitation, and the precipitated extract was filtered again using a Buchner funnel lined with Whatman No. 2 filter paper to separate only the precipitate. The separated precipitate was completely dried in a dry oven at 60°C to separate the crude phytate solids. Crude phytate is a form of phytic acid salt, and is in the form of calcium, magnesium, zinc, or sodium salts, with the main components being the calcium and magnesium salts of phytic acid.

[0116] The solid material obtained by drying, in the form of crude phytate, was redissolved in a 1% citric acid solution to produce a liquid, which was then treated with activated carbon to remove pigments and impurities. Subsequently, metal ions were removed using a cation exchange resin, and then the solution was concentrated via a vacuum concentrator to a phytic acid-containing solution based on the soluble solids content to 40 Brix. The cation exchange resin used for removing metal ions was TRILITE MC-08 (Samyang, Co., Korea), and the specifications of the ion exchange resin are as shown in Table 9 below.

[0117] [Table 9]

[0118] 4. Analysis of inorganic ion content The inorganic content remaining in a phytic acid-containing solution purified using an ion exchange resin was confirmed by inorganic ion analysis.

[0119] Inorganic ions were analyzed using an ion chromatography system, the Metrohm MagIc Net system, and detected using a Conductivity Detector. For anion analysis, a Metrosep A supp 5 column (Metrohm, 150x4mm) was used, with a mobile phase consisting of a solution containing 3.2 mM sodium carbonate and 1.0 mM sodium bicarbonate, flowed at a rate of 0.7 mL / min. For cation analysis, a Metrosep C4 column (Metrohm, 150x4mm) was used, with a mobile phase consisting of a mixture of 0.7 mM dipicolinic acid and 1.7 mM nitric acid, flowed at a rate of 0.9 mL / min. The sample pretreatment method involved adding 9.9 mL of distilled water to 0.1 mL of the sample solution and mixing thoroughly. This was followed by centrifugation (10,000 rpm, 10 min, 4°C), after which the supernatant was filtered through a 0.25 μm syringe filter. 10 μL of this supernatant was then injected and analyzed.

[0120] The results of the analysis of inorganic ions before purification with ion exchange resin are shown in Table 10 below, and the results of the analysis of inorganic ions after purification with ion exchange resin are shown in Table 11 below.

[0121] [Table 10]

[0122] [Table 11]

[0123] In general, metal ions are known to be used as catalysts in enzymatic activity or to enhance enzyme activity by structurally benefiting the enzyme's function. The majority of cations are Na. + , K + Ca 2+ Mg 2+It is known that various factors are involved, and in the case of rice bran extract extracted using enzymes, it is thought that metal ions were partially or completely utilized depending on the type of enzyme and its mechanism of action, which is also seen as beneficial in terms of improving the efficiency of purification. Relatively speaking, the concentration of metal ions decreased after purification with ion exchange resin, and generally, extraction methods using acid showed a higher residual metal ion component compared to extraction methods using enzymes. Furthermore, solutions extracted using acid as the main extraction solvent showed a high retention rate of metal ions. [Examples]

[0124] [Experimental Example 1] Measurement of antioxidant capacity - Measurement of DPPH radical scavenging activity and degree of browning

[0125] The DPPH radical scavenging activity and degree of browning were measured for the phytic acid-containing solution prepared in Production Example 2.

[0126] The DPPH radical scavenging activity was measured using the Blois method. A 4 mM DPPH (DPPH(1,1-diphenyl-2-picrylhydrazyl, Sigma Chemical Co.)) ethanol solution was prepared and its absorbance was adjusted to 1.000 ± 0.1 before use. Specifically, 0.2 mL of each sample was added to a test tube, mixed with 2.8 mL of DPPH solution, reacted for 10 minutes, and then measured at 517 nm using a Microplate reader (M2, Molecular Device, Canada). The DPPH radical scavenging activity was calculated from the following formula. DPPH radical scavenging activity(%)=[1-(A / B)]X100 (A: Absorbance of the group with added sample, B: Absorbance of the group without added sample)

[0127] To measure the degree of browning in the phytic acid-containing solution, the absorbance was measured at 420 nm using a spectrophotometer (U-2900, HITACHI, Co., Japan) without concentrating the solution immediately after preparation. The measurement results for DPPH radical scavenging activity and degree of browning are shown in Table 12 below.

[0128] [Table 12]

[0129] As shown in Table 12, the DPPH scavenging activity, which indicates antioxidant activity, was high for all enzymes used in the extraction. Of the enzymes used, Ultraflo Max showed the highest activity at 80.57%, while Shearzyme plus showed the lowest activity. The degree of browning was at a similar level regardless of the type of enzyme used. In addition, to investigate the change in the degree of browning of phytic acid-containing solutions over storage period, an Ultraflo Max-treated solution with a high phytic acid content was obtained from a phytic acid-containing solution obtained by removing metal ions. This solution was concentrated to a concentration of 40-41 Brix, and the absorbance (Abs) was measured at 15-day intervals while storing it at 10°C to check the change in the color of the solution. At this time, phytic acid-containing solutions obtained using hydrochloric acid extraction and sulfuric acid extraction were compared under the same conditions as control groups. The absorbance measurement results for each solution are shown in Table 13 below.

[0130] [Table 13]

[0131] The degree of browning of phytic acid-containing solutions obtained by enzymatic treatment was examined, and it was shown that there was no significant change due to the storage period. Specifically, in the case of the phytic acid-containing solution obtained by enzymatic extraction, the absorbance measured on day 0 was 0.123, and the absorbance measured on day 75 was 0.132, an increase of 0.009. In contrast, the absorbance of the phytic acid-containing solution obtained by hydrochloric acid extraction was 0.195 on day 0, and the absorbance measured on day 75 was 0.228, an increase of 0.033. The absorbance of the phytic acid-containing solution obtained by sulfuric acid extraction was 0.285 on day 0, and the absorbance measured on day 75 was 0.305, an increase of 0.02. It was confirmed that the change in the degree of browning (negative hue change) of the phytic acid-containing solution obtained by enzymatic extraction was at the lowest level. [Examples]

[0132] [Experimental Example 2] Measurement of phytic acid content, antioxidant capacity, and transparency

[0133] The phytic acid content was measured in the phytic acid-containing solution produced in Production Example 2, and the phytic acid content was also measured for Competitor Product 1 (sulfuric acid treatment method) and Competitor Product 2 (hydrochloric acid treatment method), which were produced using sulfuric acid or hydrochloric acid, and the results are shown in Table 14 below.

[0134] Furthermore, the antioxidant capacity of the phytic acid-containing solution and other companies' products manufactured using the acid produced in Manufacturing Example 2 was measured via DPPH radical scavenging ability and ABTS analysis. DPPH radical scavenging ability was measured using the same method as described in Experimental Example 1.

[0135] The ABTS analysis method was modified from the method of Re et al. (ReR., N.Pellegrini, A.Proteggente, A.Pannala, M.Yang and C.Rice-Evans 1999, Free Radic Biol Med. 26:1231-1237). The ABTS solution was prepared by mixing the sample with an ABTS solution containing 7.4 mM ABTS and 2.45 mM K2S2O8, reacting it at room temperature for 6 minutes, and then measuring the absorbance at 734 nm.

[0136] The measurement results of the antioxidant capacity of the phytic acid-containing solution and the competitor's product are shown in Table 14 below. As shown in the measurement results, it can be confirmed that the antioxidant capacity of the phytic acid-containing solution of Production Example 2 of this application is superior to that of the competitor's product.

[0137] [Table 14]

[0138] Furthermore, Figure 1 shows comparative photographs of the phytic acid-containing solution from Production Example 2 with competitor products 1 (sulfuric acid treatment method) and 2 (hydrochloric acid treatment method). From Figure 1, it can be seen that the phytic acid-containing solution from Production Example 2 of this application has a lower degree of browning and superior transparency compared to competitor products 1 and 2, which were produced by acid treatment.

[0139] [Experimental Example 3] Measurement of antibacterial activity

[0140] To confirm whether the phytic acid-containing solution of Production Example 2 has regulatory activity against fermenting microorganisms, its antibacterial effect against fermenting microorganisms was measured. Among the rice bran extracts extracted using enzymes, Ultraflo Max, which had the highest phytic acid content and high antioxidant efficacy, was used to produce a phytic acid-containing solution. The antibacterial effect against fermenting microorganisms was then investigated. Rice bran extract produced by acid treatment was used as the control group.

[0141] 1. Strain culture The bacterial strains used in the experiment were Lactobacillus sakei KCTC3598 and Leuconostoc mesenteroides KCTC3505, which are kimchi-derived lactic acid bacteria, and were subcultured every 24 hours at 35°C using MRS Broth as the culture medium. Bacillus subtilis KCTC1021, a microorganism derived from traditional soy sauces, was subcultured every 24 hours every 24 hours at 35°C under anaerobic conditions using TSB as the culture medium.

[0142] 2. Paper disc diffusion method To measure the antimicrobial activity of the samples, paper disc diffusion was performed to identify the growth inhibition rings for each bacterial strain. For Lactobacillus sakei (KCTC3598) and Leuconostoc mesenteroides (KCTC3505), the turbidity was adjusted with 0.5 McFarland standard, and MRS broth medium was added and poured onto MRS agar medium. Once the medium had solidified, an 8 mm diameter paper disc containing the sample solution was placed on top, and after incubation at 35°C for 24 hours, the size of the growth inhibition rings formed was measured. For Bacillus subtilis (KCTC1021), the turbidity was adjusted with 0.5 McFarland standard, and TS broth medium was added and poured onto TS agar medium. Once the culture medium had hardened, an 8mm diameter paper disc containing the sample solution was placed on top, and after anaerobic incubation at 37°C for 48 hours, the size of the growth inhibition ring that formed was measured.

[0143] 3.Minimum inhibitory concentration The minimum inhibitory concentration (MIC) refers to the minimum concentration of an antibiotic that inhibits the growth of a microorganism. Therefore, a lower MIC value for a microorganism indicates that the sample material is more susceptible to that microorganism. MIC measurement was performed using a modified liquid medium dilution method. 100 μL of medium, diluted to a two-fold concentration by two times each, was placed in a 96-well plate using the two-fold dilution method, along with 1.0 x 10⁴ oz. 6 100 μL of bacteria adjusted to CFU / mL was added and incubated at 35°C for 24 hours to determine the minimum concentration at which bacterial growth was not observed.

[0144] 4. Minimum bacterial concentration Since the MIC (Micromicrobial Limit) is the minimum inhibitory concentration for bacteria and does not necessarily mean that all bacteria have been killed, to confirm the minimum bacterial mortality concentration (MBC), sample cultures with an MIC value or higher were streaked onto solid medium and incubated. The MBC value was then defined as the concentration at which no bacteria grew at all, after which the formation of colonies was checked. The minimum bacterial mortality concentration was confirmed by ripping the sample culture from a 96-well plate (where the MIC had progressed) using a loop and incubating it at 35°C for 24 hours.

[0145] 5. Measurement results of antibacterial effect - disk diffusion method To confirm the antibacterial effect on fermenting microorganisms, the clear zone (mm), which is the growth inhibition ring for bacteria, was measured. The results are shown in Table 15 and Figure 2 below.

[0146] [Table 15]

[0147] Lactobacillus sakei showed growth inhibition rings of 1.05 mm–1.31 mm, with no significant difference between extraction methods. Leuconostoc mesenteroides showed growth inhibition rings of 0.48 mm–0.62 mm, with the sulfuric acid (H2SO4) extraction method exhibiting the greatest antibacterial effect. Bacillus subtilis showed the best growth inhibition ring and highest antibacterial effect with the enzyme extraction method.

[0148] 6. Measurement results of antibacterial effect - minimum inhibitory concentration and minimum inactivating concentration The results of confirming the minimum inhibitory and minimum inactivating concentrations of rice bran extract are shown in Table 16 below.

[0149] [Table 16]

[0150] The concentration of rice bran extract that exhibited antibacterial effects showed similar trends across all extraction methods, as demonstrated by the disk diffusion method. For Lactobacillus sakei, the minimum inhibitory concentration of the extract was 625 ul / ml for all extraction methods, while the minimum inactivating concentration increased to 1250 ul / ml except for the hydrochloric acid (HCl) extraction method. For Leuconostoc mesenteroides, the minimum inhibitory concentration was 312 ul / ml for all extraction methods except the sulfuric acid (H2SO4) extraction method, but the minimum inactivating concentration increased to a higher 1250 ul / ml. For Bacillus subtilis, both the minimum inhibitory and inactivating concentrations were 625 ul / ml, indicating that it exhibited antibacterial effects at the lowest concentration among fermenting microorganisms.

[0151] Based on the antimicrobial experimental results described above, it was confirmed that the phytic acid-containing rice bran extract obtained by the enzyme treatment method exhibited equivalent or superior antimicrobial activity against fermentation microorganisms compared to the rice bran extract obtained by the acid treatment method. The phytic acid-containing rice bran extract obtained by the enzyme treatment method of this application can be used as a regulatory substance to control the degree of fermentation by fermentation microorganisms by regulating the growth and death of fermentation microorganisms.

[0152] While the above describes representative embodiments of this application, the scope of this application is not limited to the specific embodiments described above, and any person with ordinary skill in the art may modify the claims of this application as appropriate.

Claims

1. A method for producing a phytic acid-containing composition from rice bran, including the following steps: (a) A step of producing a rice bran enzyme extract by treating rice bran with an enzyme without treating it with hydrochloric acid or sulfuric acid, wherein the enzyme is an enzyme having one or more activities selected from the group consisting of pullulanase, lipase, and protease activity; (b) A step in which the rice bran enzyme extract is neutralized to produce a precipitate; (c) The step of redissolving the precipitate to produce a redissolved product; and (d) The step of bringing the redissolved material into contact with the ion exchange resin.

2. A method for producing a composition containing phytic acid from rice bran according to claim 1, wherein the enzyme in step (a) is an enzyme further having one or more activities selected from the group consisting of beta-glucanase, endo-1,3(4)-beta-glucanase [beta-glucanase(endo-1,3(4)-)], cellulase, hemicellulase, xylanase, beta-xylanase, endo-1-4-beta-xylanase, alpha-amylase, and alpha-amylase activity.

3. A method for producing a composition containing phytic acid from rice bran according to claim 1, wherein the time for processing the enzyme in step (a) is 0.1 to 16 hours.

4. A method for producing a composition containing phytic acid from rice bran according to claim 1, wherein the enzymatic treatment in step (a) is carried out under conditions of a pH of 4.3-5.

5.

5. A method for producing a composition containing phytic acid from rice bran according to claim 1, wherein the enzymatic treatment in step (a) is carried out at a temperature of 35-55°C.

6. A method for producing a composition containing phytic acid from rice bran according to claim 1, further comprising the step of filtering the rice bran enzyme extract after step (a) and before step (b).

7. The method for producing a composition containing phytic acid from rice bran according to claim 1, wherein the neutralization of the rice bran enzyme extract in step (b) adjusts the pH of the rice bran enzyme extract to a range of 7-7.

5.

8. The Na of the redissolved product in step (c) ＋ , NH4 ＋ , K ＋ , Ca ２＋ , Mg ２＋ , Cl － , PO ４ ３－ , and SO ４ ２－ A method for producing a composition containing phytic acid according to claim 1, wherein the total concentration of the ions of is 1900 ppm or less.

9. A method for producing a composition containing phytic acid from rice bran according to claim 1, wherein the redissolution of the precipitate in step (c) is carried out by adding one or more selected from the group consisting of citric acid, lactic acid, malic acid, and tartaric acid to the precipitate.

10. A method for producing a composition containing phytic acid from rice bran according to claim 1, wherein in step (d), the ion exchange resin is a cation exchange resin, an anion exchange resin, or a combination thereof.

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