Organ perfusion device

Abstract

An organ perfusion device (1) includes: a chamber (100) that is open to the atmosphere and houses an organ; an inflow channel (220) that receives a perfusion fluid to be sent to the organ; and an outflow channel (210) that can be connected at one end to an inlet blood vessel of the organ to send the perfusion fluid received by the inflow channel toward the inlet blood vessel. The chamber (100) includes a connecting port (120) that is openable / closable and configured to be connectable to the inlet flow channel (220). In the organ perfusion device (1), an open system circuit is formed by connecting an end (401) that receives the perfusion fluid in the inflow channel (220) to the connecting port (120), and a closed system circuit is formed by closing the connecting port and connecting an end (402) that receives the perfusion fluid in the inflow channel to an outlet blood vessel of the organ.

Description

Technical Field

[0001] The present invention relates to an organ perfusion device.

Background Art

[0002] As a device for removing an organ for transplantation and preserving it while maintaining its function, an organ perfusion device for perfusing a perfusion solution into the organ has been developed.

[0003] Organ perfusion is performed using an open-system circuit in which at least a part of the circuit through which the perfusion solution circulates is open to the atmosphere, or using a closed-system circuit in which no part open to the atmosphere is provided on the circuit through which the perfusion solution circulates.

[0004] Japanese Unexamined Patent Application Publication No. 2020-002062 (Patent Document 1) discloses an organ perfusion device in which one end of an inflow pipe is connected to the renal artery of a kidney and one end of an outflow pipe is connected to the renal vein of the kidney.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0006] Conventionally, organ perfusion devices have been designed specifically for each circuit. Therefore, the circuits available to users of organ perfusion devices were inevitably determined by the type of organ perfusion device at hand.

[0007] However, the inventors have found that there are advantages and disadvantages in the open-system circuit and the closed-system circuit respectively, and there is a problem in that the available circuit is determined by the type of organ perfusion device.

[0008] One objective of this disclosure is to provide an organ perfusion device that allows users to arbitrarily select between an open circuit and a closed circuit. [Means for solving the problem]

[0009] The organ perfusion device of this disclosure is an organ perfusion device for perfusing an organ with a perfusion fluid. The organ perfusion device is open to the atmosphere and includes a chamber for housing an organ, an inflow channel connected to the organ's inlet vessel, and one or more outflow channels fluid-connected to the inflow channel and located on the organ's outlet vessel side. The chamber includes a connecting port configured to be openable and closable and connectable to one or more outflow channels. One or more outflow channels are configured to be selectively connectable to either the connecting port or the outlet vessel. In the organ perfusion device, an open circuit is formed by connecting one or more outflow channels to the connecting port, and a closed circuit is formed by connecting one or more outflow channels to the outlet vessel. [Effects of the Invention]

[0010] According to this disclosure, users of the organ perfusion device can arbitrarily select between an open circuit and a closed circuit. [Brief explanation of the drawing]

[0011] [Figure 1] This is a schematic diagram showing the overall configuration of the organ perfusion system. [Figure 2] This is a schematic diagram showing the overall configuration of an organ perfusion device with an open circuit. [Figure 3] This is a schematic diagram showing the overall configuration of an organ perfusion device in which a closed-system circuit is formed. [Figure 4] This is a side view of the chamber. [Figure 5] This is a diagram showing a part of a closed-loop circuit. [Figure 6] This is a flowchart showing the replenishment method. [Modes for carrying out the invention]

[0012] The embodiments of this disclosure will be described in detail below with reference to the drawings. In the drawings, the same or corresponding parts are denoted by the same reference numerals, and their descriptions will not be repeated.

[0013] [Overall configuration of the organ perfusion system] Figure 1 is a schematic diagram showing the overall configuration of the organ perfusion device 1. The organ perfusion device 1 is a device for flowing perfusion fluid into organs extracted from a donor in order to preserve and / or evaluate the organs extracted from the donor. The "donor" may be a human or a non-human animal. The "organ" may be any organ that has an inlet vessel for blood to flow in and an outlet vessel for blood to flow out, such as the heart, kidney, lung, pancreas, stomach, small intestine, large intestine, testes, ovaries, or eyeball. Note that the organ only needs to have one or more inlet vessels and one or more outlet vessels. In this embodiment, a liver 10 extracted from a human will be used as an example of an organ. The "perfusion fluid" is appropriately selected according to the purpose of perfusion, and may be, for example, physiological saline, organ preservation solution, or blood.

[0014] The organ perfusion device 1 comprises a chamber 100 for receiving the liver 10, an outflow channel 210 located on the outlet blood vessel side of the liver 10, a reservoir 400 for storing perfusion fluid from the outflow channel 210, and an inflow channel 220 connected to the inlet blood vessel of the liver 10. Although the two channels are not directly connected, the reservoir 400 is located between the outflow channel 210 and the inflow channel 220, and the two channels are fluidically connected.

[0015] Chamber 100 is a container for housing the liver 10. Since an opening 102 is provided at the top of chamber 100, the inside of chamber 100 is open to the atmosphere and exposed to the air. Chamber 100 may also be further provided with a lid covering the opening 102. Chamber 100 only needs to be open to the atmosphere of the space in which it is installed. For example, if chamber 100 is installed in a nitrogen gas atmosphere, chamber 100 will be open to the nitrogen gas atmosphere.

[0016] The chamber 100 is configured to be openable and closable and includes a connection port 120 configured to be connectable to the outflow channel 210. The connection port 120 is configured to be able to attach both the cap 120a and the outflow channel 210.

[0017] In addition, a waste liquid channel 240 for sending the perfusion liquid in the chamber 100 to a waste liquid tank (not shown) is connected to the chamber 100. The waste liquid channel 240 includes a waste liquid valve 242 for opening and closing the waste liquid channel 240. By opening the waste liquid valve 242, the perfusion liquid in the chamber 100 can be sent to the waste liquid tank through the waste liquid channel 240. Details of the structure of the chamber 100 will be described later with reference to FIGS. 4 and 5.

[0018] The outflow channel 210 is a channel for receiving the perfusion liquid discharged from the liver 10. One end of the outflow channel 210 is configured to be connectable to the reservoir 400, and the other end is configured to be selectively connectable to either the connection port 120 or the hepatic vein 13.

[0019] In the present embodiment, the outflow channel 210 includes a first connection channel 212 and a second connection channel 214. One end of the first connection channel 212 is configured to be connectable to a connector 401 formed in the reservoir 400, and the other end is configured to be connectable to the connection port 120. One end of the second connection channel 214 is configured to be connectable to a connector 402 formed in the reservoir 400, and the other end is configured to be connectable to the hepatic vein 13.

[0020] The reservoir 400 is a container for storing the perfusion liquid and is a sealed container. The outflow channel 210 and the inflow channel 220 are connected to the reservoir 400. The perfusion liquid sent from the outflow channel 210 is sent to the reservoir 400. The perfusion liquid sent to the reservoir 400 is sent from the reservoir 400 to the liver 10 through the inflow channel 220.

[0021] The reservoir 400 is equipped with a liquid level sensor 410 for measuring the liquid level of the perfusion fluid inside the reservoir 400. A replenishment channel 230 is also connected to the reservoir 400. The replenishment channel 230 receives the perfusion fluid to be replenished in the reservoir 400 and replenishes the reservoir 400 with the perfusion fluid. The replenishment channel 230 is equipped with a replenishment valve 232 for opening and closing the replenishment channel 230.

[0022] In this embodiment, the reservoir 400 includes at least five connectors 401 to 405. Connector 401 is configured to be connectable to the first connection channel 212. Connector 402 is configured to be connectable to the second connection channel 214. Connector 403 is configured to be connectable to the first inflow channel 222 of the inflow channel 220. Connector 404 is configured to be connectable to the second inflow channel 224 of the inflow channel 220. Connector 405 is configured to be connectable to the replenishment channel 230.

[0023] The inflow channel 220 is connected to the inlet blood vessels of the organ, the liver 10, and is a channel that delivers perfusion fluid to the liver 10. One end of the inflow channel 220 is connected to the inlet blood vessels of the organ, and the other end is connected to the reservoir 400. The liver 10 has two inlet blood vessels, the hepatic artery 11 and the portal vein 12. Therefore, in this embodiment, the inflow channel 220 comprises a first inflow channel 222 connected to the hepatic artery 11 and a second inflow channel 224 connected to the portal vein 12. The first inflow channel 222 is a channel that connects the reservoir 400 and the hepatic artery 11. The second inflow channel 224 is a channel that connects the reservoir 400 and the portal vein 12.

[0024] With the first inflow channel 222 and the second inflow channel 224 connected to the liver 10 in this manner, the perfusion fluid in the reservoir 400 flows through the first inflow channel 222 from the hepatic artery 11 to the liver 10, and through the second inflow channel 224 from the portal vein 12 to the liver 10. The bile produced in the liver 10 is sent to the container 252 through the bile channel 250 connected to the bile duct 14.

[0025] Furthermore, the organ perfusion device 1 includes a pump 300, a supply device 600, a flow meter 700, and a pressure gauge 800.

[0026] Pump 300 is a device for sending perfusion fluid from reservoir 400 to the inlet vessel, and in this embodiment, it is located in the inflow channel 220. When pump 300 is driven, the perfusion fluid in reservoir 400 is sent to the inlet vessel through the inflow channel 220. In this embodiment, the organ perfusion device 1 includes a first pump 310 and a second pump 320 as the pump 300. The first pump 310 is a device for sending perfusion fluid from reservoir 400 to the hepatic artery 11, and is located in the first inflow channel 222. The second pump 320 is a device for sending perfusion fluid from reservoir 400 to the portal vein 12, and is located in the second inflow channel 224.

[0027] The supply device 600 supplies oxygen to the perfusion fluid sent to the organs and, in this embodiment, is located in the inflow channel 220. For example, the supply device 600 is an artificial lung that supplies oxygen to the perfusion fluid to increase the oxygen concentration in the perfusion fluid. In this embodiment, the organ perfusion device 1 includes a first supply device 610 and a second supply device 620 as the supply device 600. The first supply device 610 supplies oxygen to the perfusion fluid flowing through the first inflow channel 222 and is located in the first inflow channel 222. The second supply device 620 supplies oxygen to the perfusion fluid flowing through the second inflow channel 224 and is located in the second inflow channel 224.

[0028] The flow meter 700 measures the flow rate of the perfusion fluid flowing through the inflow channel 220. In this embodiment, the organ perfusion device 1 includes a first flow meter 710 that measures the perfusion fluid flowing through the first inflow channel 222 and a second flow meter 720 that measures the perfusion fluid flowing through the second inflow channel 224, as the flow meter 700.

[0029] The pressure gauge 800 measures the pressure of the perfusion fluid flowing through the inflow channel 220. In this embodiment, the organ perfusion device 1 includes a first pressure gauge 810 that measures the pressure of the perfusion fluid flowing through the first inflow channel 222, and a second pressure gauge 820 that measures the pressure of the perfusion fluid flowing through the second inflow channel 224, as the pressure gauge 800.

[0030] The control device 500 includes a CPU (Central Processing Unit) 501 which is an arithmetic unit, RAM (Random Access Memory) 502, and a storage device 503.

[0031] The CPU 501 controls the operation of each part of the organ perfusion device 1 by reading and executing a program stored in the memory device 503. For example, the CPU 501 controls the pump by executing the program. Although Figure 1 illustrates a configuration with a single CPU 501, the organ perfusion device 1 may have a configuration with multiple CPUs.

[0032] The storage device 503 is implemented by a non-volatile storage device such as ROM (Read Only Memory) or a hard disk. The storage device 503 stores programs executed by the CPU 501, or data used by the CPU 501. The programs may be stored on a non-temporary computer-readable medium.

[0033] The control device 500 receives measurement results from the liquid level sensor 410, the first flow meter 710, the second flow meter 720, the first pressure gauge 810, and the second pressure gauge 820. The control device 500 controls the opening and closing of the replenishment valve 232 according to the measurement results of the liquid level sensor 410. The control device 500 controls the first pump 310 according to the measurement results of the first flow meter 710 and the first pressure gauge 810, and controls the second pump 320 according to the measurement results of the second flow meter 720 and the second pressure gauge 820.

[0034] Furthermore, the control device 500 according to this embodiment is connected to an input device 504 for inputting information that can identify whether the circuit assembled in the organ perfusion device 1 is an open system circuit or a closed system circuit. The input device 504 may accept input by mechanical operation such as a switch. In addition, if a display device such as a liquid crystal display is further provided, the input device 504 may be composed of a touch panel, keyboard, mouse, etc.

[0035] [Open-circuit and closed-circuit systems] The organ perfusion device 1 comprises an outflow channel 210 configured to be selectively connectable to either a connecting port 120 or a hepatic vein 13, and a chamber 100 having the connecting port 120. In the organ perfusion device 1, an open circuit is formed by connecting the outflow channel 210 to the connecting port 120, and a closed circuit is formed by connecting the outflow channel 210 to the hepatic vein 13 of the liver 10. The open circuit and the closed circuit will be described below with reference to Figures 2 and 3.

[0036] Figure 2 is a schematic diagram showing the overall configuration of an organ perfusion device with an open circuit. Figure 3 is a schematic diagram showing the overall configuration of an organ perfusion device with a closed circuit. In Figures 2 and 3, some reference numerals have been omitted for simplicity.

[0037] (Open system circuit) Referring to Figure 2, an open-circuit system is formed by connecting the end of the outflow channel 210 that receives the perfusion fluid to the connecting port 120. More specifically, one end of the first connecting channel 212 is connected to the connector 401 formed on the reservoir 400, and the other end of the first connecting channel 212 is connected to the connecting port 120. The second connecting channel 214 is not needed in the open-circuit system and therefore does not need to be connected to the connector 402. A cap 402a is attached to the connector 402.

[0038] The perfusion fluid in chamber 100 is sent to reservoir 400 through the first connecting channel 212 from the connecting port. The perfusion fluid in reservoir 400 is sent to the liver 10 from the hepatic artery 11 or portal vein 12 through the first inflow channel 222 and the second inflow channel 224, respectively, as the first pump 310 and the second pump 320 are driven. The perfusion fluid sent to the liver 10 is discharged into chamber 100 by opening to the atmosphere through the hepatic vein 13. The discharged perfusion fluid is then sent back to the liver 10 through the first connecting channel 212, reservoir 400, first inflow channel 222, and second inflow channel 224. In this way, an open system circuit is formed by connecting the first connecting channel 212 to the connecting port 120.

[0039] (Closed circuit) Referring to Figure 3, a closed system circuit is formed by connecting the end of the outflow channel 210 that receives the perfusion fluid to the hepatic vein 13 and closing the connecting port 120. More specifically, one end of the second connecting channel 214 is connected to the connector 402 formed on the reservoir 400, and the other end of the second connecting channel 214 is connected to the hepatic vein 13. The first connecting channel 212 is not needed in the closed system circuit and therefore does not need to be connected to the connector 401. A cap 401a is attached to the connector 401.

[0040] Furthermore, an infusion bag 234 is connected to the replenishment channel 230, and the replenishment valve 232 is opened and closed according to the measurement results of the liquid level sensor 410, and perfusion fluid is replenished from the infusion bag 234. For a detailed explanation of the replenishment method, please refer to Figure 6 and see the following description.

[0041] The perfusion fluid sent to the liver is discharged into the reservoir 400 through the second connecting channel 214 without being released to the atmosphere from the hepatic vein 13. Because the reservoir 400 is a sealed container, the perfusion fluid is discharged into the reservoir 400 without being released to the atmosphere. The perfusion fluid in the reservoir 400 is sent to the liver 10 in conjunction with the operation of the first pump 310 and the second pump 320, similar to an open circuit. In this way, a closed circuit is formed by closing the connecting port 120 and connecting the second connecting channel 214 to the hepatic vein 13.

[0042] (Advantages and disadvantages of open-circuit and closed-circuit systems) In an open circuit, the hepatic vein 13 is open to the atmosphere, thus reducing the risk of excessive pressure inside the liver 10. Furthermore, reagent administration and perfusion fluid replenishment can be performed directly from the open-air chamber 100, making these operations easy.

[0043] However, in an open circuit, the perfusion fluid returned to the liver 10 is exposed to the atmosphere within the chamber 100. Therefore, there is a risk of contamination. In addition, since the perfusion fluid is discharged into the chamber 100 from the hepatic vein 13 and then returned to the liver 10, it is necessary to fill not only the flow path but also the chamber 100 with perfusion fluid, and thus an open circuit requires a large amount of perfusion fluid.

[0044] On the other hand, in a closed circuit, the risk of contamination is low because the perfusion fluid is not exposed to the atmosphere. Also, since it is not necessary to fill the chamber 100 with the perfusion fluid used for perfusion, the total amount of perfusion fluid used can be reduced, making it easier to analyze metabolites from the liver 10 in a closed circuit.

[0045] However, in a closed circuit, since it is not open to the atmosphere, there is a risk of excessive pressure being applied inside the liver 10, and it is necessary to construct a control system to prevent excessive pressure from being applied. Also, because the perfusion fluid flows through a closed circuit, it is time-consuming to administer reagents to the perfusion fluid or to replenish the perfusion fluid.

[0046] As described above, open and closed circuits each have their own advantages and disadvantages. The organ perfusion device 1 according to this embodiment, as shown in Figures 2 and 3, can be configured as either an open or closed circuit by providing an openable / closable connecting port 120 in the chamber 100 that can be connected to an inflow channel 220. Therefore, by using the organ perfusion device 1 according to this embodiment, users such as physicians can consider the advantages and disadvantages of each circuit and appropriately select the circuit according to the condition of the organ and the purpose of perfusion.

[0047] [Chamber structure] Referring to Figure 4, the detailed structure of the chamber 100 will be described. Figure 4 is a side view of the chamber. The chamber 100 has a protrusion 140 formed on at least a portion of its bottom surface, projecting outward from the side opposite to the side that houses the liver 10.

[0048] The protrusion 140 has a connecting port 120 and a waste liquid port 122. Both the connecting port 120 and the waste liquid port 122 are provided facing outwards from the chamber 100 and have a cylindrical shape.

[0049] A cylindrical connector 130 is provided in the chamber 100 so as to penetrate its side. The connector 130 provided on the side of the chamber will be described with reference to Figure 5. Figure 5 is a diagram showing a part of the closed system circuit. Note that in Figure 5, some reference numerals and drawings have been omitted for the sake of simplicity.

[0050] Referring to Figure 5, the connector 130 includes a first connecting portion 132 provided inward from the wall of the chamber 100 and a second connecting portion 134 provided outward from the wall of the chamber 100.

[0051] The second connecting channel 214 includes two channels 214a and 214b connected by the connector 130. Channel 214a is a cannula configured so that one end can be attached to the hepatic vein 13. The other end of channel 214a can be connected to the first connection part 132 of the connector 130. Channel 214b can be connected to the second connection part 134 of the connector 130 and to the connector 402 of the reservoir 400.

[0052] By connecting the two channels 214a and 214b via the connector 130 in this manner, a second connecting channel 214 is formed, and the second connecting channel 214 can be attached to the hepatic vein 13.

[0053] In this embodiment, the connecting port 120 is formed on the bottom surface of the chamber 100. In an open circuit, the perfusion fluid is discharged directly into the chamber 100 from the hepatic vein 13. In this case, if the hepatic vein 13 is above the level of the perfusion fluid, the problem of foaming of the perfusion fluid occurs. In this embodiment, by forming the connecting port 120 on the bottom surface of the chamber 100, it is possible to prevent air bubbles from entering the flow path from the connecting port 120, and as a result, it is possible to prevent air bubbles from entering the liver 10.

[0054] Furthermore, in this embodiment, the connecting port 120 is formed on a protrusion 140 provided at the bottom of the chamber 100. This prevents the liver 10 from coming into contact with the connecting port 120, and as a result, it prevents the liver 10 from being damaged by irregularities on the connecting port 120, or from being subjected to suction pressure from the first connecting channel 212 if the connecting port 120 is blocked by the liver 10.

[0055] Furthermore, in this embodiment, the cross-sectional area A1 of the first connecting channel 212 for the open circuit (see Figure 2) is larger than the cross-sectional area A2 of the second connecting channel 214 for the closed circuit (see Figure 3). Therefore, the outer diameter a1 of the connecting port 120 is larger than the outer diameter a2 of the connector 130. For example, the outer diameter a1 of the connecting port 120 is 9 mm to 18 mm, and the outer diameter a2 of the connector 130 is 6 mm to 15 mm.

[0056] By increasing the cross-sectional area of ​​the first connecting channel 212, the pressure loss in the first connecting channel 212 can be reduced, and the atmospheric pressure acting on the perfusion fluid in the chamber 100 can efficiently send the perfusion fluid in the chamber 100 to the reservoir 400.

[0057] [Control when using a closed-loop circuit] If liver 10 is in poor condition, it may accumulate perfusion fluid. In such cases, the amount of perfusion fluid sent to liver 10 and the amount of perfusion fluid coming out of liver 10 are not balanced, causing the amount of perfusion fluid in reservoir 400 to decrease, and in the worst case, it may become empty. If pump 300 is operated when there is no perfusion fluid in reservoir 400, air will enter liver 10.

[0058] In an open-system circuit, the perfusion fluid can be easily replenished from the top of the chamber 100, but in a closed-system circuit, the perfusion fluid cannot be replenished from the chamber 100. Furthermore, since the reservoir 400 is also sealed, it is not easy to replenish the perfusion fluid. In addition, in a closed-system circuit, the total amount of perfusion fluid flowing through the circuit is less than in an open-system circuit, so the risk of the reservoir 400 running out is higher than in an open-system circuit.

[0059] In this embodiment, the control device 500, when a closed-loop circuit is assembled, monitors the liquid level of the reservoir 400 with a liquid level sensor 410, and replenishes the reservoir 400 with perfusion fluid when the liquid level of the reservoir 400 falls below a predetermined threshold.

[0060] The replenishment method will be explained with reference to Figure 6. Figure 6 is a flowchart showing the replenishment method. Each process (step) shown in Figure 6 is executed by the control device 500, and is realized by the CPU 501 executing various programs stored in the storage device 503.

[0061] The control device 500 determines whether the assembled circuit is a closed system circuit based on the information input from the input device 504. If the control device 500 determines that the assembled circuit is not a closed system circuit, in other words, if it determines that it is an open system circuit, it does not execute the process shown in Figure 6. If the control device 500 determines that the assembled circuit is a closed system circuit, it starts the process shown in Figure 6. The control device 500 may also automatically detect the assembled circuit. For example, a detector capable of detecting whether a flow path is connected to each of the connectors 401 and 402 may be provided, and the control device 500 may detect the assembled circuit based on the detection results of the detector. The control device 500 may also start the process shown in Figure 6 even if an open system circuit is assembled.

[0062] In step S1, the control device 500 determines, based on the measurement results of the liquid level sensor 410, whether the liquid level of the reservoir 400 is lower than a predetermined first threshold H1.

[0063] If the control device 500 determines that the liquid level in reservoir 400 is not less than the first threshold H1 (NO in step S1), in other words, if it determines that the liquid level in reservoir 400 is equal to or greater than the first threshold H1, the control device 500 proceeds to step S3. If the control device 500 determines that the liquid level in reservoir 400 is lower than the first threshold H1 (YES in step S1), the control device 500 proceeds to step S2.

[0064] In step S2, the control device 500 opens the replenishment valve 232. This allows perfusion fluid to be replenished from the infusion bag 234 containing the perfusion fluid to the reservoir 400 through the replenishment channel 230. The infusion bag 234 is positioned, for example, vertically above the reservoir 400. This allows gravity to replenish the perfusion fluid. IV bag The current flows from 234 towards reservoir 400.

[0065] In step S3, the control device 500 determines, based on the measurement results from the liquid level sensor 410, whether the liquid level in the reservoir 400 is equal to or greater than a predetermined second threshold H2. The second threshold H2 is set to a value greater than the first threshold H1.

[0066] If the control device 500 determines that the liquid level in reservoir 400 is not equal to or greater than the second threshold H2 (NO in step S3), in other words, if it determines that the liquid level in reservoir 400 is less than the second threshold H2, the control device 500 terminates the process. If the control device 500 determines that the liquid level in reservoir 400 is equal to or greater than the second threshold H2 (YES in step S3), the control device 500 proceeds to step S4.

[0067] In step S4, the control device 500 closes the replenishment valve 232 and terminates the process. IV bagThe supply of perfusion fluid from 234 to reservoir 400 will stop.

[0068] The control device 500 performs the process shown in Figure 6 at predetermined intervals. This maintains the fluid level of the perfusion fluid in the reservoir 400 at or above the first threshold H1. As a result, it is possible to prevent the reservoir 400 from becoming empty and air from being supplied to the liver 10. Furthermore, the fluid level of the perfusion fluid in the reservoir 400 is maintained below the second threshold H2. As a result, the total amount of perfusion fluid used in the closed circuit can be kept within a predetermined range.

[0069] [Differentiation] In the above embodiment, the organ was assumed to be the liver 10. Since the liver 10 has two inlet blood vessels, the organ perfusion device 1 is provided with a first inlet channel 222 and a second inlet channel 224 as the inlet channel 220. The organ perfusion device 1 is provided with one inlet channel when targeting an organ with only one inlet blood vessel, and with three inlet channels when targeting an organ with three inlet blood vessels. In other words, the number of inlet channels should be provided according to the number of inlet blood vessels of the target organ.

[0070] Furthermore, when targeting organs with two outlet vessels, the organ perfusion device 1 only needs to be equipped with two second connecting channels 214 for closed-system circuits. In other words, the number of outflow channels (connecting channels) for closed-system circuits should be provided according to the number of outlet vessels of the target organ.

[0071] In the above embodiment, the organ perfusion device 1 includes a first pump 310 for flowing perfusion fluid through a first inflow channel 222 and a second pump 320 for flowing perfusion fluid through a second inflow channel 224. However, the organ perfusion device 1 may also achieve the functions of both the first pump 310 and the second pump 320 with a single pump.

[0072] In the above embodiment, the organ perfusion device 1 is equipped with a reservoir 400. However, the organ perfusion device 1 does not need to be equipped with a reservoir 400. In this case, the outflow channel 210 and the inflow channel 220 can be connected via a single channel. Any device or the like may be provided between the outflow channel 210 and the inflow channel 220, as long as the outflow channel 210 and the inflow channel 220 are fluidly connected.

[0073] In the above embodiment, the organ perfusion device 1 has connectors 401 and 402 formed on the reservoir 400 in order to increase the cross-sectional area of ​​the flow path connected to the connection port 120, and uses the first connecting flow path 212 and the second connecting flow path 214 depending on the circuit. However, if the cross-sectional area of ​​the flow path connected to the connection port 120 and the flow path connected to the hepatic vein 13 is the same, it is not necessary to use different flow paths depending on the circuit, and only one connector on the outflow flow path 210 side formed on the reservoir 400 is needed. In other words, in the above embodiment, the outflow flow path 210 only needs to be configured to be selectively connected to either the connection port 120 or the outlet vessel, and may consist of a single connecting flow path.

[0074] In the above embodiment, the reservoir 400 is provided with at least five connectors. However, the reservoir 400 only needs to have connectors for connecting the outflow channel 210 and the inflow channel 220, and may have two or more connectors. The number of connectors provided by the reservoir 400 should be set according to the number of channels that may be connected to the reservoir 400, and is not limited to five.

[0075] [Pattern] Those skilled in the art will understand that the embodiments described above are specific examples of the following embodiments.

[0076] (Section 1) An organ perfusion device according to one embodiment is an organ perfusion device that perfuses an organ with a perfusion fluid. The organ perfusion device is open to the atmosphere and includes a chamber for housing an organ, an inflow channel connected to the organ's inlet vessel, and one or more outflow channels fluid-connected to the inflow channel and located on the organ's outlet vessel side. The chamber includes a connecting port that is configured to be openable and closable and connectable to one or more outflow channels. One or more outflow channels are configured to be selectively connectable to either the connecting port or the outlet vessel. In the organ perfusion device, an open system circuit is formed by connecting one or more outflow channels to the connecting port, and a closed system circuit is formed by connecting one or more outflow channels to the outlet vessel.

[0077] According to the organ perfusion device described in paragraph 1, the user of the organ perfusion device can arbitrarily select between an open system circuit and a closed system circuit.

[0078] (Section 2) In the organ perfusion apparatus described in Section 1, the connecting port is formed at the bottom of the chamber.

[0079] According to the organ perfusion device described in paragraph 2, when an open circuit is formed, even if the perfusion fluid discharged from the outlet blood vessel is directly discharged into the chamber, it is possible to prevent the bubbles from entering the flow path through the connection port, and as a result, it is possible to prevent bubbles from entering the organ.

[0080] (Section 3) In the organ perfusion apparatus described in Section 2, the chamber includes a projection formed on its bottom surface and projecting outward from the side of the chamber that receives the organ. The connecting port is formed on the projection.

[0081] The organ perfusion device described in paragraph 3 prevents damage to organs due to irregularities in the connecting port, and prevents suction pressure from the inflow channel from being applied to organs if the connecting port is blocked by an organ.

[0082] (Section 4) The organ perfusion device described in any one of Sections 1 to 3 further includes a reservoir for storing perfusion fluid. One or more outflow channels include a first channel connected to the reservoir and selectively connected to a connecting port to form an open circuit, and a second channel connected to the reservoir and selectively connected to an outlet vessel to form a closed circuit. The cross-sectional area of ​​the first connecting channel is greater than the cross-sectional area of ​​the second connecting channel.

[0083] According to the organ perfusion apparatus described in Section 4, the pressure loss can be reduced by increasing the cross-sectional area of ​​the first connecting channel, and when an open circuit is formed, the atmospheric pressure acting on the perfusion fluid in the chamber can efficiently send the perfusion fluid in the chamber to the reservoir.

[0084] (Section 5) The organ perfusion apparatus described in Section 4 further includes a replenishment channel connected to a reservoir for receiving the perfusion fluid to be replenished in the reservoir, a valve for opening and closing the replenishment channel, a liquid level sensor for measuring the liquid level of the perfusion fluid in the reservoir, and a control device. The control device performs replenishment control by opening the valve and replenishing the perfusion fluid in the reservoir when the liquid level is below a first threshold.

[0085] The organ perfusion device described in paragraph 5 prevents the reservoir from becoming empty and air from being supplied to the organs.

[0086] (Section 6) In the organ perfusion device described in Section 5, the control device performs supplementation control when a closed system circuit is formed.

[0087] According to the organ perfusion device described in Section 6, the total amount of perfusion fluid flowing through the circuit is less than in an open circuit, and replenishment control is performed in a closed circuit where there is a high risk of the reservoir running out, thus reducing the risk of the reservoir running out.

[0088] The embodiments disclosed herein should be considered in all respects to be illustrative and not restrictive. The scope of the present invention is indicated by the claims rather than by the description of the embodiments above, and all modifications within the meaning and scope of the claims are intended to be included. [Explanation of Symbols]

[0089] 1 Organ perfusion device, 10 Liver, 11 Hepatic artery, 12 Portal vein, 13 Hepatic vein, 14 Bile duct, 100 Chamber, 102 Opening, 120 Connecting port, 120a, 401a, 402a Cap, 122 Drain port, 130, 401~405 Connector, 132 First connection, 134 Second connection, 140 Protrusion, 210 Outflow channel, 212 First connection channel, 214 Second connection channel, 220 Inflow channel, 222 First inflow channel, 224 Second inflow channel, 230 Refill channel, 232 Refill valve, 234 Infusion bag, 240 Drain channel, 242 Drain valve, 250 Bile channel, 252 Container, 300 Pump, 310 First pump, 320 Second pump, 400 Reservoir, 410 Liquid level sensor, 500 control device, 503 memory device, 504 input device, 600 supply device, 610 first supply device, 620 second supply device, 700 flow meter, 710 first flow meter, 720 second flow meter, 800 pressure gauge, 810 first pressure gauge, 820 second pressure gauge.

Claims

1. An organ perfusion device that perfuses organs with perfusion fluid, It is open to the atmosphere and includes a chamber for housing the aforementioned organs, An inflow channel connected to the inlet blood vessel of the aforementioned organ, The inflow channel is fluid-connected to one or more outflow channels located on the exit blood vessel side of the organ, The chamber is configured to be openable and closable and includes a connecting port configured to be connected to one or more outflow channels, The one or more outflow channels are configured to be selectively connectable to either the connecting port or the outlet blood vessel. An open circuit is formed by connecting one or more of the aforementioned outflow channels to the connecting port. An organ perfusion device in which a closed system circuit is formed by connecting one or more outflow channels to the outlet blood vessels.

2. The organ perfusion device according to claim 1, wherein the connecting port is formed at the bottom of the chamber.

3. The chamber includes a protrusion formed on its bottom surface and projecting outward from the side of the chamber that receives the organ, The organ perfusion device according to claim 2, wherein the connecting port is formed in the protrusion.

4. The system further includes a reservoir for storing the perfusion fluid, The one or more outflow channels are A first channel connected to the reservoir and selectively connected to the connecting port to form the open circuit, It includes a second channel connected to the reservoir and selectively connected to the outlet blood vessel to form the closed system circuit, The organ perfusion device according to claim 1, wherein the cross-sectional area of ​​the first channel is larger than the cross-sectional area of ​​the second channel.

5. A replenishment channel connected to the reservoir and receiving the perfusion fluid to be replenished in the reservoir, A valve for opening and closing the aforementioned replenishment channel, A liquid level sensor for measuring the liquid level height of the perfusion fluid in the reservoir, Further equipped with a control device, The organ perfusion apparatus according to claim 4, wherein the control device performs replenishment control by opening the valve and replenishing the perfusion fluid in the reservoir when the liquid level is below a first threshold.

6. The organ perfusion device according to claim 5, wherein the control device performs the replenishment control when the closed system circuit is formed.

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