Plant activator

Abstract

To provide a plant activator with excellent workability and reduced transport cost.SOLUTION: A solid plant activator contains at least one compound selected from oxo fatty acid or a derivative thereof or a salt thereof and hydroxylated fatty acid or a derivative thereof or a salt thereof, and a carbohydrate.SELECTED DRAWING: None

Description

[Technical Field]

[0001] This invention relates to a plant activator. [Background technology]

[0002] Technologies for regulating plant growth have been developed with the aim of improving the supply efficiency of grain plants and horticultural plants. In addition to measures such as optimizing temperature and sunlight conditions and fertilization, methods have been reported for revitalizing plants using plant stimulants that have plant growth regulating effects such as growth promotion, dormancy suppression, and stress reduction.

[0003] Patent Document 1 reports a plant activator characterized by containing oxo fatty acids or their salts or esters as active ingredients. The plant activator described in Patent Document 1 has excellent resistance-inducing and growth-promoting effects on plants. [Prior art documents] [Patent Documents]

[0004] [Patent Document 1] International Publication No. 2018 / 168860 [Overview of the project] [Problems that the invention aims to solve]

[0005] However, the plant activator described in Patent Document 1 has the problem that the active ingredient is dispersed and dissolved in water, and there are transportation costs involved in preparing the plant activator and transporting it to farmland.

[0006] The present invention aims to provide a plant activator that can reduce transportation costs and exhibit a high plant activating effect. [Means for solving the problem]

[0007] As a result of intensive research, the inventors of the present invention have come up with the idea that by removing water, which is a solvent that significantly contributes to transportation costs, and solidifying the fatty acid, which is the active ingredient as a plant activator dispersed and dissolved in water, by retaining it with a retaining agent, the transportation costs can be reduced.

[0008] Also, simply removing water as a solvent only causes new problems. Since the fatty acid, which is the active ingredient as a plant activator, has a lipophilic group, the solids aggregate with each other, or when the solids are packed in bags for storage and transportation, the solid fatty acid adheres to the inner wall of the bag, or even when trying to spray the solid fatty acid using a powder sprayer, it clogs the nozzles and hoses. However, the inventors of the present invention have found that by using a carbohydrate as a retaining agent, aggregation of the solids with each other can be prevented, and adhesion of the solids to the inner wall of the bag for storage and transportation can also be prevented, thus completing the present invention.

[0009] The present invention relates to a solid plant activator containing at least one compound selected from oxo fatty acids or their derivatives or their salts and hydroxy fatty acids or their derivatives or their salts, and a carbohydrate.

[0010] It is desirable that the solid plant activator is in powder or granular form.

[0011] The oxo fatty acid or its derivative or its salt is the following formula (I): HOOC-(R 1 )-CH=CH-C(=O)-R 2 (I) (In the formula, R 1 : is a linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 1 1 2 carbon atoms, R 2 : is an alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and / or double bonds) A plant activator which is an oxo fatty acid or its derivative or its salt having the structural formula is preferred.

[0012] The oxo fatty acid, where the R of the oxo fatty acid 1 has a hydrocarbon group with 8 to 10 carbon atoms, and the activator for plants which is an oxo fatty acid where the alkyl group of R 2 has 4 to 6 carbon atoms is preferred.

[0013] The oxo fatty acid, where the R of the oxo fatty acid 1 forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I), and the activator for plants which is an oxo fatty acid containing such a double bond is preferred.

[0014] The oxo fatty acid, where the R of the oxo fatty acid 1 is a linear or branched hydrocarbon group with 9 carbon atoms, and the activator for plants which is an oxo fatty acid where R 2 is an alkyl group with 5 carbon atoms is preferred.

[0015] The activator for plants where the oxo fatty acid is keto octadecadienoic acid is preferred.

[0016] The activator for plants where the oxo fatty acid is 13 - oxo - 9,11 - octadecadienoic acid is preferred.

[0017] The hydroxy fatty acid or its derivative or its salt is the following formula (II) and / or (III): HOOC-(R 3 )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R 4 (II) HOOC-(R 3 )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R 4 (III) (In the formula, R 3 is a linear or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and / or OH groups, and when containing a double bond, the position of the double bond is not limited, R 4(This refers to a linear or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and / or OH groups, and if it contains double bonds, the position of the double bonds is not limited.) A plant activator that is a hydroxylated fatty acid or a derivative thereof having the structural formula is preferred.

[0018] The hydroxylated fatty acid, 3 The hydrocarbon group has 6 to 8 carbon atoms, R 4 A plant activator having a hydrocarbon group with 4 to 6 carbon atoms is preferred.

[0019] The hydroxylated fatty acid, 3 However, -(CH2) n -(n is an integer between 4 and 12) is the structure, R 4 However, C n H 2n+1 A plant activator with the structure -(where n is an integer between 2 and 8) is preferred.

[0020] The hydroxylated fatty acid, 3 However, it is a straight-chain saturated hydrocarbon group (-(CH2)7-) with 7 carbon atoms, and R 4 However, a plant activator that is an alkyl group with 5 carbon atoms (CH3CH2CH2CH2CH2-) is preferred.

[0021] A plant activator in which the hydroxylated fatty acid is hydroxyoctadecenoic acid is preferred.

[0022] A plant activator is preferred in which the hydroxylated fatty acid is 9,10,13-trihydroxy-11-octadecenoic acid (structural formula (1) below).

[0023] [ka]

[0024] A plant activator is preferred in which the hydroxylated fatty acid is 9,12,13-trihydroxy-10-octadecenoic acid (structural formula (2) below).

[0025] [ka]

[0026] Furthermore, the derivatives of the oxo fatty acid and hydroxylated fatty acid are preferably esters of the oxo fatty acid and hydroxylated fatty acid, respectively. Also, as the salts of the oxo fatty acid and hydroxylated fatty acid, as will be described later, salts such as sodium salts, potassium salts, and ammonium salts can be used.

[0027] A plant activator is preferred in which the carbohydrate is at least one selected from polysaccharides, sugar alcohols, and dietary fiber.

[0028] Preferably, the carbohydrate is a polysaccharide, and the polysaccharide is at least one selected from pullulan and dextrin.

[0029] Preferably, the carbohydrate is a sugar alcohol, and the sugar alcohol is at least one selected from xylitol, erythritol, and maltitol.

[0030] The carbohydrate is preferably dietary fiber, and the dietary fiber is preferably at least one selected from cellulose and polydextrose.

[0031] The aforementioned plant activator is preferably a plant activator used for plants selected from the Brassicaceae, Poaceae, Fabaceae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae families.

[0032] The carbohydrates used in this invention are preferably aggregates of primary particles or fibers. [Effects of the Invention]

[0033] The plant activator of the present invention exhibits high disease resistance induction and growth promotion effects, is easy to work with, and can reduce transportation and storage costs. [Modes for carrying out the invention]

[0034] Plant activator The plant activator of the present invention is characterized by comprising at least one compound selected from "oxo fatty acids or their derivatives or salts" and "hydroxylated fatty acids or their derivatives or salts" and a carbohydrate, and being in solid form.

[0035] In this invention, "plant activation" means adjusting the growth activity of plants in some way to activate or maintain it, and is a concept that includes growth promotion (a concept that encompasses the expansion of stems and leaves, the promotion of tuber and root growth, etc.), dormancy suppression, induction and conferral of resistance to plant stress (e.g., diseases), and plant growth regulatory effects such as anti-aging.

[0036] The plant activator of the present invention contains at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts as an active ingredient for plant activating effects. At least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts can impart a growth-promoting effect to plants when applied to the stems, leaves, or roots of a plant. In plants inoculated with the plant activator of the present invention, compared to untreated plants, an increase in leaf length and leaf weight (indicators of plant growth), and promotion of tuber or root growth are observed, suggesting that the plant activator of the present invention imparts a growth-promoting effect to plants. By using the plant activator of the present invention, plant growth can be promoted, leading to increased yields of vegetables, grains, fruits, and other plants. The plant growth-promoting effect of the plant activator of the present invention is very high, resulting in excellent yield increases and improved harvesting efficiency for commercial crops. Furthermore, by applying the plant activator of the present invention to the stems, leaves, or roots of a plant, it can activate the salicylic acid pathway, which is involved in resistance induction in the plant, and as a result, it can induce resistance to diseases and other pests in the plant.

[0037] In the plant activator of the present invention, at least one compound selected from the above-mentioned oxo fatty acid or its derivative or salt and hydroxylated fatty acid or its derivative or salt is an active ingredient that exhibits plant activating activity. The present invention is characterized by containing this active ingredient that exhibits plant activating activity and carbohydrates, and being in solid form. Because the active ingredient that exhibits plant activating activity is a fatty acid and has lipophilic groups, even if the water present as a solvent in the plant activator is dried and removed to form solid components (powder, granules), adhesion between lipophilic groups occurs, resulting in poor fluidity, aggregation of solid components, and adhesion of solid components to the inner walls of storage and transport bags and containers, making them difficult to remove, or adhesion to nozzles and hoses when sprayed with a powder sprayer. In the present invention, it is presumed that the active ingredient that exhibits plant activating activity is retained in the voids within the primary particles and fiber aggregates that constitute the carbohydrates, so the solid components do not adhere to each other and aggregate, making them easy to handle. Furthermore, the solid components do not adhere to the inner walls of storage and transport bags or containers, making them easy to remove and improving work efficiency. In addition, when spraying with a powder sprayer, they do not adhere to nozzles or hoses, thus reducing the labor required for spraying the solid components.

[0038] The carbohydrates used in this invention are preferably at least one selected from polysaccharides, sugar alcohols, and dietary fiber.

[0039] Examples of polysaccharides include starch (amylose, amylopectin), glycogen, dextrin, cyclodextrin, gellan gum, carrageenan, pullulan, curdlan, xanthan gum, tamarind seed gum, chitosan, and titanium gum. The polysaccharides may consist of one or more types. In the present invention, it is desirable that the polysaccharide used is at least one selected from pullulan and dextrin.

[0040] Examples of sugar alcohols include sorbitol, erythritol, xylitol, mannitol, galactitol, maltitol, isomaltitol, lactitol, maltotriitol, isomalttriitol, panitol, and maltotetraitol. One or more sugar alcohols may be included. Preferably, the sugar alcohol used in the present invention is at least one selected from xylitol, erythritol, and maltitol.

[0041] Examples of dietary fiber include isomaltodextrin, hemicellulose, pectin, polydextrose, alginic acid and its salts, laminarin, guar gum, guar gum hydrolysate, glucomannan, inulin, fucoidan, cellulose, lignin, protopectin, cellulose derivatives in which some or all of the hydroxyl groups, such as carboxymethylcellulose, hydroxypropylcellulose, and cellulose acetate, are substituted with substituents such as methoxy or acetoxy groups, gum arabic, and locust bean gum. The dietary fiber may consist of one or more types. Preferably, the dietary fiber used in this invention is at least one selected from cellulose and polydextrose.

[0042] These carbohydrates, although composed of primary particles and fibers aggregated to form larger particles or powders, are thought to retain fatty acids in the gaps between primary particles and fibers. It is estimated that the surface of the solid component of the present invention, obtained by drying a plant activator containing carbohydrates, is substantially covered with carbohydrates. As a result, the solid component is given good fluidity, preventing aggregation of solid components and adhesion to the walls of bags or containers. Furthermore, even when the powder of the solid component is sprayed using a powder sprayer, nozzles and hoses do not become clogged, thus enabling labor savings while maintaining the plant activating effect.

[0043] The carbohydrate content in the plant activator of the present invention is preferably 10 to 5000 times the weight ratio of the content of oxo fatty acids or their derivatives or salts and at least one compound selected from hydroxylated fatty acids or their derivatives or salts. This is because it prevents aggregation of solid components and adhesion of solid components to bags and containers without impairing the function of the plant activator.

[0044] The solid component of the plant activator of the present invention is preferably in powder or granular form, as this improves handling and workability. If it is in powder form, the average particle size is preferably 0.1 to 500 μm. If it is in granular form, the average particle size is preferably greater than 0.5 mm and 30 mm or less.

[0045] In this invention, the average particle size of the solid component is determined by measuring the particle size distribution using a laser diffraction / scattering particle size distribution analyzer in a dry measurement manner, and the average particle size is defined as the particle size at the point when the volume has accumulated to 50%.

[0046] The powder or granular solid component having the above-mentioned average particle diameter or average particle shape may be composed of secondary particles (granular particles) formed by the aggregation of primary particles. The formation of moderately aggregated secondary particles makes it more difficult for fatty acid molecules to be exposed on the surface of the particles, which can improve the fluidity of the solid component.

[0047] The solid component of the plant activator of the present invention may further contain a solid component consisting of a pH buffer. Examples of pH buffers that can be used include salts and organic acids such as tripotassium phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, potassium carbonate, potassium bicarbonate, sodium carbonate, sodium bicarbonate, sodium citrate, trisodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium tripolyphosphate, and ammonium dihydrogen phosphate.

[0048] As described above, the plant activator of the present invention comprises at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts. The oxo fatty acid or its derivative or salt of the present invention is the following formula (I): HOOC-(R 1 )-CH=CH-C(=O)-R 2 (I) (In the formula, R 1 : A linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms. R 2 (An alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and / or double bonds.) Oxo fatty acids having the structural formula shown, or their derivatives or salts thereof, can be suitably used.

[0049] In one embodiment of the present invention, R in the oxo fatty acid 1 The hydrocarbon group has 8 to 10 carbon atoms, R 2 The alkyl group has 4 to 6 carbon atoms. In another embodiment, the R in the above oxo fatty acid 1 The double bond in formula (I) contains a double bond that forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group. Furthermore, in another embodiment, the R in the oxo fatty acid is 1 R is a linear or branched hydrocarbon group having 9 carbon atoms. 2 It is preferable that it is an alkyl group having 5 carbon atoms.

[0050] Specifically, examples of oxo fatty acids in the present invention include ketooctadecadienoic acid. For example, while not limited to these, examples of ketooctadecadienoic acid include 9-oxo-10,12-octadecadienoic acid (9-oxoODA), 13-oxo-9,11-octadecadienoic acid (13-oxoODA), 5-oxo-6,8-octadecadienoic acid, 6-oxo-9,12-octadecadienoic acid, 8-oxo-9,12-octadecadienoic acid, 10-oxo-8,12-octadecadienoic acid, 11-oxo-9,12-octadecadienoic acid, 12-oxo-9,13-octadecadienoic acid, and 14-oxo-9,12-octadecadienoic acid, as well as their isomers.

[0051] Furthermore, esters are preferred as derivatives of oxo fatty acids. The esters of oxo fatty acids of the present invention are not limited to these, but examples include methyl esters, ethyl esters, propyl esters, butyl esters, pentyl esters, isopentyl esters, octyl esters, etc. As for salts of oxo fatty acids, examples include ammonium salts such as alkylammonium salts such as ammonium salts and tetramethylammonium salts, alkaline earth metal salts such as calcium salts and magnesium salts, alkali metal salts such as sodium salts, lithium salts and potassium salts, and metal salts such as cobalt salts and manganese salts, but are not particularly limited as long as they are one or more agriculturally acceptable salts, such as salts contained in fertilizers.

[0052] The hydroxylated fatty acid or its derivative or salt thereof according to the present invention is the following formula (II) and / or (III) HOOC-(R 3 )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R 4 (II) HOOC-(R 3 )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R 4 (III) (In the formula, R 3This refers to a linear or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and / or OH groups, and if it contains double bonds, the position of the double bonds is not limited. R 4 (This refers to a linear or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and / or OH groups, and if it contains double bonds, the position of the double bonds is not limited.) Hydroxylated fatty acids having the structural formula shown, or their derivatives or salts thereof, can be suitably used.

[0053] In one embodiment of the present invention, R in the above hydroxylated fatty acid 3 The hydrocarbon group has 6 to 8 carbon atoms, R 4 The hydrocarbon group has 4 to 6 carbon atoms. In another embodiment, the R in the above hydroxylated fatty acid 3 is, -(CH2) n -(n is an integer between 4 and 12) is the structure, R 4 C n H 2n+1 -(n is an integer between 2 and 8) structure. Furthermore, in another embodiment, R in the above hydroxylated fatty acid 3 R is a straight-chain saturated hydrocarbon group (-(CH2)7-) with 7 carbon atoms, 4 It is preferable that the element is a C5 alkyl group (CH3CH2CH2CH2CH2-).

[0054] Specifically, examples of hydroxylated fatty acids in the present invention include hydroxyoctadecenoic acid. For example, examples of hydroxyoctadecenoic acid include, but are not limited to, 9,10,13-trihydroxy-11-octadecenoic acid and / or 9,12,13-trihydroxy-10-octadecenoic acid and their isomers.

[0055] Furthermore, esters are preferred as derivatives of hydroxylated fatty acids. The esters of hydroxylated fatty acids of the present invention are not limited to these, but examples include methyl esters, ethyl esters, propyl esters, butyl esters, pentyl esters, isopentyl esters, octyl esters, etc. As for salts of hydroxylated fatty acids, examples include ammonium salts such as alkylammonium salts such as ammonium salts and tetramethylammonium salts, alkaline earth metal salts such as calcium salts and magnesium salts, alkali metal salts such as sodium salts, lithium salts and potassium salts, and metal salts such as cobalt salts and manganese salts, but are not particularly limited as long as they are one or more agriculturally acceptable salts, such as salts contained in fertilizers.

[0056] In addition, if isomers exist for the compounds exemplified herein, all possible isomers can be used in the present invention unless otherwise specified.

[0057] The plant activator of the present invention may contain at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts, as described above. That is, for example, it may contain two or more oxo fatty acids or their derivatives or salts or hydroxylated fatty acids or their derivatives or salts, or it may contain at least one oxo fatty acid or its derivative or salt and at least one hydroxylated fatty acid or its derivative or salt.

[0058] The method for retaining at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts in a carbohydrate according to the present invention is not particularly limited. For example, a solid plant activator containing at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts in a carbohydrate can be obtained by dissolving or dispersing a carbohydrate in a solution in which at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts is dissolved or dispersed in a suitable solvent, and then removing the solvent. The solvent used is not particularly limited.

[0059] Methods for removing the solvent include spray drying, freeze-drying, hot air drying, and vacuum drying. Therefore, the plant activator of the present invention may be a dried solid (dried powder) containing oxo fatty acids or their derivatives or salts, and at least one compound selected from hydroxylated fatty acids or their derivatives or salts, and carbohydrates. The solid content of the dried powder is 80% or more, preferably 90% or more. Transportation costs can be significantly reduced.

[0060] The oxo fatty acid or its derivative or salt thereof and at least one compound selected from the hydroxylated fatty acid or its derivative or salt thereof may be used individually or in combination.

[0061] In the plant activator of the present invention, the solid plant activator comprising an oxo fatty acid or its derivative or salt thereof, at least one compound selected from a hydroxylated fatty acid or its derivative or salt thereof, and a carbohydrate may be in tablet form or coated with a coating layer. This is because it can improve water resistance, durability, and shelf life.

[0062] The coating layer may be formed using a permeable substance such as polyamide resin or cellulose nitrate, and for example, natural polymers such as polyvinyl alcohol or gelatin can be used.

[0063] The plant activator of the present invention may optionally contain other components other than at least one compound selected from oxo fatty acids or their derivatives or salts and hydroxylated fatty acids or their derivatives or salts. Such other components include, but are not limited to, agriculturally acceptable additives such as fatty acids, surfactants, binders, solvents, absorbents, degradation inhibitors, inorganic salts, and excipients.

[0064] The ease of handling of the plant activator of the present invention is evaluated by its angle of repose. The angle of repose is the angle between the slope of the pile of powder that forms when 10 g of powder is freely dropped from a funnel of a certain height and remains stable without spontaneously collapsing, and the horizontal plane. In the case of powders with high cohesiveness, the pile becomes higher, and consequently the angle of repose increases. Therefore, in the present invention, the smaller the angle of repose, the lower the cohesiveness and the less likely it is to adhere to the inner wall surface of bags or containers. The angle of repose of the solid plant activator of the present invention is, for example, about 55° or less, preferably about 50° or less, and generally 25° or more. When the angle of repose is below the above upper limit, the powder has good fluidity. As an example of measuring the angle of repose, it can be performed in accordance with JIS R 9301-2-2 using a powder property evaluation device (for example, an ABD powder property measuring instrument manufactured by Tsutsui Rikagakukikai Co., Ltd.), and can be measured by the method described in the examples.

[0065] The plant activator of the present invention can be applied to plants by any method. For example, it can be used by uniformly spreading it directly on a field or by spraying it directly into the water of a paddy field. It can also be used as a planting hole treatment agent, furrow spraying agent, base-of-plant spraying agent, or box treatment agent. Furthermore, the form (dosage form) of the plant activator of the present invention is not particularly limited, and can be, for example, a powder (general powder, DL powder, flow dust, etc.), granules, or powder-granules (fine granules, fine granules F, etc.), and may also be in the form of granules, sheets, blocks, and films, which can be manufactured according to known manufacturing methods. On plants to which it has been applied, the plant activator of the present invention is easy to handle and easy to spray by machine, thus enabling labor savings.

[0066] The plants to which the plant activator of the present invention can be applied are not particularly limited, and it can be used effectively on plants in general. For example, it can be suitably applied to plants of the Brassicaceae, Poaceae, Fabaceae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae families. Furthermore, the plants to which it can be applied are not limited to wild-type plants, but may also be mutants or transgenic plants, for example. In addition, the varieties of each plant are not particularly limited.

[0067] Furthermore, we have found that the plant activator of the present invention can be used as a plant activator that exhibits a strong resistance-inducing effect, and has been shown to have growth-promoting effects, fruit yield-increasing effects, and disease-suppressing effects on various plants. For example, specific examples of its effectiveness in suppressing diseases include gray mold, fusarium wilt, vine blight, and downy mildew on the leaves of cucurbitaceous plants such as cucumbers, watermelons, melons, and pumpkins; bacterial wilt, wilt, verticillium wilt, damping-off, and brown root rot on solanaceous plants such as tomatoes, eggplants, and potatoes; powdery mildew, black spot, gray mold, and anthracnose on roses and strawberries; downy mildew on Amaranthaceous plants such as spinach; black rot, soft rot, bacterial leaf spot, and rhizoctonia on brassicas such as Chinese cabbage, cabbage, and komatsuna; white mold on carrots and other Apiaceae plants; and rice blast on grasses. [Examples]

[0068] The present invention will be described based on examples, but the present invention is not limited to these examples.

[0069] Example 1 (1) 100 mL of 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid (200 mg / L ethanol solution, manufactured by La Rhodan Fine Chemicals) and 100 mL of 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (200 mg / L ethanol solution, manufactured by La Rhodan Fine Chemicals) were dissolved in water to make 1 L, and a 0.004 wt% solids solution was prepared. (2) 25 g of pullulan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to the solution prepared in (1) and stirred until dissolved. The weight ratio of pullulan to hydroxylated fatty acids, which are plant activator components, was 625 times. (3) The solution prepared in (2) was placed in a crystallization dish in a 1 L container, frozen in a deep freezer at -80°C for 16 hours, then covered with a Kimtowel and secured with a rubber band, and freeze-dried for 48 hours in a freeze-dryer (FDU-1200, manufactured by Tokyo Rikakikai Co., Ltd.). The freeze-dryer was then stopped, and the mixture was powdered to obtain approximately 25 g of plant activator.

[0070] Example 2 (1) 100 mL of 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid (200 mg / L ethanol solution, manufactured by La Rhodan Fine Chemicals) and 100 mL of 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (200 mg / L ethanol solution, manufactured by La Rhodan Fine Chemicals) were dissolved in water to make 1 L, and a 0.004 wt% solids solution was prepared. (2) 25 g of pullulan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to the solution prepared in (1) and stirred until dissolved. The weight ratio of pullulan to hydroxylated fatty acids, which are plant activator components, was 625 times. (3) The solution prepared in (2) was heated with hot air at a temperature of 145°C and an airflow of 0.7 m³. 3Using a spray drying apparatus (SD-1000, manufactured by Yamato Scientific Co., Ltd.) with a flow rate of 4.5 g / min, the plant activator powder of the present invention was spray-dried to obtain approximately 20 g.

[0071] Example 3 (1) 120 mL of 13-oxo-9,11-octadecadienoic acid (13-oxoODA) ((9Z,11E)-13-oxo-9,11-octadecadienoic acid, manufactured by Cayman Chemical, 100 μg / 100 μL ethanol solution) was dissolved in water to make 1 L, and a 0.012 wt% 13-oxoODA emulsion solution was prepared. (2) 25 g of pullulan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added as a carbohydrate to the solution prepared in (1), and stirred to dissolve. The weight ratio of pullulan to oxo fatty acids was 208 times. (3) The solution prepared in (2) was placed in a crystallization dish in a 1 L container, frozen in a deep freezer at -80°C for 16 hours, then covered with a Kimtowel and secured with a rubber band, and freeze-dried for 48 hours in a freeze-dryer (FDU-1200, manufactured by Tokyo Rikakikai Co., Ltd.). The freeze-dryer was then stopped, and the mixture was powdered to obtain approximately 25 g of plant activator.

[0072] Example 4 (1) 120 mL of 13-oxo-9,11-octadecadienoic acid (13-oxoODA) ((9Z,11E)-13-oxo-9,11-octadecadienoic acid, manufactured by Cayman Chemical, 100 μg / 100 μL ethanol solution) was dissolved in water to make 1 L, and a 0.012 wt% 13-oxoODA emulsion solution was prepared. (2) 25 g of pullulan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added as a carbohydrate to the solution prepared in (1), and stirred to dissolve. The weight ratio of pullulan to oxo fatty acids was 208 times. (3) The solution prepared in (2) was heated with hot air at a temperature of 145°C and an airflow of 0.7 m³. 3 Using a spray drying apparatus (SD-1000, manufactured by Yamato Scientific Co., Ltd.) with a flow rate of 4.5 g / min, the plant activator powder of the present invention was spray-dried to obtain approximately 20 g.

[0073] Example 5 The procedure is basically the same as in Example 1, but instead of pullulan as a carbohydrate, 25g of sodium alginate (Kimika Algin BL-2, manufactured by Kimika Co., Ltd.), a dietary fiber, was dispersed and used.

[0074] Example 6 The procedure was basically the same as in Example 1, but instead of pullulan, 25g of erythritol (manufactured by Mitsubishi Chemical Foods Corporation), a sugar alcohol, was dissolved and used as the carbohydrate.

[0075] Example 7 The procedure was basically the same as in Example 1, but instead of pullulan, 25g of dextrin (manufactured by San-ei Sugar Refining Co., Ltd.) was dissolved and used as the carbohydrate.

[0076] Comparative Example 1 (1) 100 mL of 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid (200 mg / L ethanol solution, manufactured by La Rhodan Fine Chemicals) and 100 mL of 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (200 mg / L ethanol solution, manufactured by La Rhodan Fine Chemicals) were dissolved in water to make 1 L, and a 0.004 wt% solids solution was prepared. (2) 25 g of dipotassium hydrogen phosphate was added to the solution prepared in (1) as a pH buffer and stirred until dissolved. (3) The solution prepared in (2) was placed in a crystallization dish in a 1 L container, frozen in a deep freezer at -80°C for 16 hours, then covered with a Kimtowel and secured with a rubber band, and freeze-dried for 48 hours in a freeze-dryer (FDU-1200, manufactured by Tokyo Rikakikai Co., Ltd.). The freeze-dryer was then stopped, and the mixture was powdered to obtain approximately 25 g of plant activator.

[0077] Comparative Example 2 (1) 120 mL of 13-oxo-9,11-octadecadienoic acid (13-oxoODA) ((9Z,11E)-13-oxo-9,11-octadecadienoic acid, manufactured by Cayman Chemical, 100 μg / 100 μL ethanol solution) was dissolved in water to make 1 L, and a 0.012 wt% 13-oxoODA emulsion solution was prepared. (2) Add 25 g of dipotassium hydrogen phosphate as a pH buffer to the solution prepared in (1), and stir until dissolved. (3) The solution prepared in (2) was placed in a crystallization dish in a 1 L container, frozen in a deep freezer at -80°C for 16 hours, then covered with a Kimtowel and secured with a rubber band, and freeze-dried for 48 hours in a freeze-dryer (FDU-1200, manufactured by Tokyo Rikakikai Co., Ltd.). The freeze-dryer was then stopped, and the mixture was powdered to obtain approximately 25 g of plant activator.

[0078] (Angle of repose measurement) The angle of repose was measured for 10 g each of the plant activator powders obtained in Examples 1-7 and Comparative Examples 1 and 2. The measurement was performed using an ABD powder property analyzer manufactured by Tsutsui Chemical Instruments Co., Ltd., by dropping each plant activator powder onto a horizontal substrate from a funnel of a certain height and measuring the angle of the resulting deposit. (Electron microscope observation) Electron microscope observation of the powders obtained in Examples 1-7 and Comparative Examples 1 and 2 suggested that they were aggregates of primary particles.

[0079] [Table 1]

[0080] As can be seen from the results in Table 1, the angles of repose in Examples 1-7, which had carbohydrates added, were smaller compared to Comparative Examples 1 and 2.

[0081] From the above results, it can be seen that the solid plant activator, which consists of an oxo fatty acid or its derivative or salt and at least one compound selected from hydroxylated fatty acids or their derivatives or salts, and a carbohydrate, has good fluidity. Therefore, the solid plant activator of the present invention is less prone to aggregation and does not adhere to storage and transport bags or containers, making it easy to handle. Furthermore, it does not clog nozzles or hoses when sprayed using a powder sprayer, thus enabling labor savings.

Claims

1. comprising 13-oxo-9,11-octadecadienoic acid or a derivative thereof or a salt thereof and pullulan, The pullulan content is 208 to 5000 times the weight ratio of the 13-oxo-9,11-octadecadienoic acid or its derivative or salt. A solid plant activator.

2. The plant activator according to claim 1, which is used for plants selected from the Brassicaceae, Poaceae, Fabaceae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae families.

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