Topical skin preparations

Abstract

To provide a novel active ingredient that is blended in an external preparation for skin, such as cosmetics, and has an effect of enhancing ceramide synthase activity.SOLUTION: A ceramide synthase activity enhancer contains one or more plant-derived components selected from extract of Callicarpa japonica, extract of Tabebuia impetiginosa, fermented product of Hemerocallis fulva var. kwanso or Hemerocallis fulva var. fulva, fermented product of Coix lachryma-jobi var. ma-yuen and extract of Luffa cylindrica.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to an active ingredient of an external preparation for skin having an effect of promoting ceramide synthase activity.

Background Art

[0002] Conventionally, due to external factors such as aging, active oxygen induced by sunlight (ultraviolet rays), chemical substances such as air pollutants and environmental hormones, allergic substances such as pollen, and environmental stress, the stratum corneum cell lipids decrease, and the amount of water evaporation from the skin increases, resulting in problems such as dryness, rough skin, and invasion of allergens. In order to solve this problem, although cosmetics containing ceramide, free fatty acid or cholesterol that constitute stratum corneum cell lipids have been proposed, there are problems in terms of effectiveness, usability and stability.

Disclosure of the Invention

Problems to be Solved by the Invention

[0003] In view of the above conventional problems, the present inventors have found a component derived from plants (plants belonging to the genus Perilla of the family Lamiaceae, plants belonging to the genus Tabebuia of the family Bignoniaceae, plants belonging to the genus Myosotis of the family Boraginaceae, plants belonging to the genus Benincasa of the family Cucurbitaceae, plants belonging to the genus Zizania of the family Poaceae) that enhances the enzyme activity for synthesizing ceramide that constitutes stratum corneum cell lipids. Conventionally, although the above plant-derived components are blended in external preparations for skin as disclosed in, for example, Patent Documents 1 to 11, it has not been known that these plant-derived components enhance the enzyme activity for synthesizing ceramide.

[0004]

Patent Document 1

[0005] The present invention is a ceramide synthase activity enhancer containing one or more plant-derived components from among the following: extract of Callicarpa japonica, extract of Tabebuia impetiginosa, fermented product of Hemerocallis fulva or Hemerocallis middendorffii, fermented product of Coix lacryma-jobi, and extract of Luffa gourd. [Effects of the Invention]

[0006] According to the present invention, it is possible to provide topical skin preparations such as cosmetics that promote the synthesis of ceramides, which constitute intercellular lipids of the stratum corneum, by enhancing the ceramide synthase activity of plant-derived components that have excellent biosafety. [Modes for carrying out the invention]

[0007] The present invention is a ceramide synthesis promoter containing one or more plant-derived components from among the following: extract of Callicarpa japonica, extract of Tabebuia impetiginosa, fermented product of Hemerocallis middendorffii or Hemerocallis fulva, extract of Luffa gourd, and fermented product of Coix lacryma-jobi. The plant materials used in the present invention are preferably the following, but the present invention is not limited thereto.

[0008] First, "Murasakishikibu" refers to the plant Callicarpa japonica, a member of the genus Callicarpa in the Lamiaceae family. The entire plant, or the flowers, fruits, stems, roots, or leaves, can be used, but the fruit is preferred. In addition, other plants of the same genus, such as Callicarpa japonica var. luxurians, Callicarpa dichotoma, Callicarpa formosana, and Callicarpa mollis, can also be used.

[0009] Next, Tabebuia impetiginosa is a plant belonging to the genus Tabebuia in the family Bignoniaceae, and it is preferable to use the whole plant or the bark (inner bark). In addition, other plants of the same genus such as Tabebuia rosea, Tabebuia caraicia, Tabebuia chrysantha, and Tabebuia chrysotricha can also be used.

[0010] Next, Honkanzou and Yabukanzou refer to plants of the genus Hemerocallis in the family Liliaceae, specifically "Honkanzou (Hemerocallis fulva var. fulva) and Yabukanzou (Hemerocallis fulva var. kwanso)". The whole plant, leaves, flowers, stamens, pistils, stems, rhizomes, and seeds (fruits) can be used, but the use of the flower part, including petals and buds, is preferred. Also, related species of Hemerocallis include Hemerocallis fulva var. littorea, Hemerocallis fulva var. longituba, Hemerocallis fulva var. sempervirens, Hemerocallis citrina Baroni, Hemerocallis dumortieri var. dumortieri, Hemerocallis lilioasphodelus L., Hemerocallis mirror, Hemerocallis lilioasphodelus var. yezoensis, Hemerocallis dumortieri var. esculenta, and Hemerocallis citrina var. Other options include using Hemerocallis plicata or Hemerocallis vespertina.

[0011] Next, loofah refers to the loofah plant, a member of the Cucurbitaceae family, genus Luffa. The entire plant, or its flowers, fruits, stems, roots, or leaves, can be used, but the fruit is preferred.

[0012] Next, Job's tears refers to Coix lacryma-jobi, a member of the grass genus Coix, and the use of Job's tears seeds is preferred. In the present invention, when using Job's tears seeds, both hulled and hulled seeds can be used, and furthermore, they can be used as whole grains, as powder obtained by crushing or pulverizing, or as high-temperature and high-pressure processed products of Job's tears seeds or powder. In any case, a fermented product with equivalent and stronger skin physiological activity than the original Job's tears seeds can be obtained. However, from the viewpoint of storage stability as a raw material and extraction and fermentation efficiency, it is preferable to use powder obtained by crushing or pulverizing, or a high-temperature and high-pressure processed product thereof, in both the case of hulled and hulled seeds.

[0013] Extracts from the above plants can be prepared, for example, as follows: First, the extraction part of each plant is washed with water if necessary to remove foreign matter, then either left as is or dried, and if necessary, finely chopped or pulverized, and then brought into contact with the extraction solvent for extraction. Extraction can be carried out by contacting the extraction solvent according to conventional methods such as immersion, but supercritical fluid extraction or steam distillation can also be used.

[0014] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; polyhydric alcohols such as ethylene glycol, 1,3-propanediol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, methyl propionate, and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether, isopropyl ether, and other etheric hydrocarbon solvents such as n-hexane, toluene, and chloroform. These can be used individually or in combination of two or more.

[0015] When using a mixed solvent, the mixing ratio, for example, in the case of a mixed solvent of water and ethyl alcohol, is preferably in the range of 1:1 to 25:1 by volume ratio (the same shall apply hereinafter), in the case of a mixed solvent of water and glycerin, it is 1:1 to 15:1, and in the case of a mixed solvent of water and 1,3 - propanediol or 1,3 - butylene glycol, it is preferably in the range of 1:1 to 15:1.

[0016] When preparing the extract, there is no particular limitation on its pH, but generally it is preferably in the range of 3 to 9. In this sense, if necessary, an alkaline regulator such as sodium hydroxide, sodium carbonate, potassium hydroxide, etc., or an acidic regulator such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, etc. may be added to the extraction solvent to adjust it to the desired pH.

[0017] The extraction conditions such as the extraction temperature and extraction time vary depending on the type of solvent and pH used. For example, when water or 1,3 - butylene glycol, or a mixture of water and 1,3 - butylene glycol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 90°C, and the extraction time is preferably 0.5 hours to 7 days.

[0018] Here, prior to or in parallel with the extraction treatment of the present invention, a hydrolysis treatment may be applied to the extraction site as necessary. This may improve the skin irritation, effectiveness, storage stability, etc. of the extract and enable more effective use of the extract.

[0019] Also, in order to enhance the storage stability, etc., the extract prepared as described above may be refrigerated for a certain period of time and then the supernatant may be used.

[0020] Furthermore, in this invention, the above-mentioned daylilies, licorice, and adlay are fermented by the following procedure. First, the daylilies, licorice, and adlay themselves may be used as the source of fermentation, or extracts obtained by extracting them with an appropriate medium may be used. When using extracts, it is also possible to carry out fermentation with the plant material still included, without removing the plant material by solid-liquid separation. The plants may be fresh, or they may be pre-dried or semi-dried. In terms of form, the collected plants may be used as they are.

[0021] In the present invention, examples of microorganisms used for fermentation include lactic acid bacteria, yeast, koji mold, or Bacillus subtilis. Examples of lactic acid bacteria include Lactobacillus species such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbrueckii; Carnobacterium species such as Carnobacterium divergens and Carnobacterium piscicola; and Leuconostoc mesenteroides, Leuconostoc lactis, and Leuconostoc citreum. Lactic acid bacteria of the genus Leuconostoc, such as *Leuconostoc citreum*; lactic acid bacteria of the genus Streptococcus, such as *Streptococcus faecalis* and *Streptococcus pyogenes*; lactic acid bacteria of the genus Enterococcus, such as *Enterococcus caseliflavus* and *Enterococcus sulfreus*; lactic acid bacteria of the genus Lactococcus, such as *Lactococcus plantarum* and *Lactococcus rafinolactis*; lactic acid bacteria of the genus Weissella, such as *Weissella confusa* and *Weissella kandleri*; Atopovium Lactobacillus species of the genus Atopobium, such as Atopobium minutum and Atopobium parvulus;Examples include lactic acid bacteria of the genus Vagococcus, such as Vagococcus fluvialis and Vagococcus salmoninarum; and lactic acid bacteria of the genus Pediococcus, such as Pediococcus damnosus and Pediococcus pentosaceus.

[0022] In addition, as yeasts, for example, yeasts of the genus Saccharomyces such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus; yeasts of the genus Galactomyces; yeasts of the genus Torulaspora such as Torulaspora delbruekii, Torulaspora fermentati, and Torulaspora rosei; and Zygosaccharomyces rouxii. Yeasts of the genus Zygosaccharomyces, such as Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces lactis; yeasts of the genus Candida, such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scottii; Aureobasidium pullulans, Aureobasidium mansonii, and Aureobasidium Examples include yeasts of the genus Aureobasideium, such as Aureobasideium microstictum. Furthermore, the yeast according to the present invention may be any of the following: sake yeast, wine yeast, beer yeast, yeast derived from plant flowers (roses, lilies, cherry blossoms, etc.), or yeast derived from the sea.

[0023] Examples of koji mold include yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, and Aspergillus sojae; black koji molds such as Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger; and red koji molds such as Monascus anka and Monascus pilosus.

[0024] Furthermore, in the present invention, examples of Bacillus subtilis include Bacillus natto, Bacillus subtilis, and Bacillus circulans.

[0025] In the present invention, hydrolysis treatment using an enzyme may be performed simultaneously with fermentation, or before or after fermentation. As the enzyme, at least one enzyme selected from proteolytic enzymes, starch-degrading enzymes, pectin-degrading enzymes, fibrinolytic enzymes, and lipid-degrading enzymes can be used.

[0026] Examples of proteolytic enzymes that can be used include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase, and aminopeptidase, bromelain, and microbial complex proteolytic enzymes (e.g., Neurase [manufactured by Amano Enzyme Co., Ltd.]).

[0027] Examples of starch-degrading enzymes that can be used include α-amylase, β-amylase, glucoamylase, and β-galactosidase.

[0028] Examples of pectin-degrading enzymes that can be used include pectinase, pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, and polygalacturonase.

[0029] Examples of cellulases that can be used include cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenanase, arginase, fucoidanase, inulase, xylanase, and ligninase.

[0030] Examples of lipid-degrading enzymes that can be used include lipase and phospholipase.

[0031] When fermenting the above-mentioned suspension or extract with microorganisms, it is necessary to sterilize the suspension before the fermentation process to remove any unwanted bacteria that could hinder fermentation. Methods for removing these unwanted bacteria include washing the fermentation material with sterilizing ethanol beforehand and then suspending it in a sterile solvent such as sterile water, or suspending the fermentation material in a solvent and then sterilizing the suspension by heat sterilization. Commonly used heat sterilization methods include autoclave sterilization, where the suspension is heated to 120-130°C for 10-20 minutes, and intermittent sterilization, where the suspension is kept at 80-90°C for 60-120 minutes, once a day for 2-3 days.

[0032] A sterilized suspension is placed in a fermentation tank, and microorganisms are inoculated into it to initiate fermentation. The amount of microorganisms inoculated is 10 7 ~10 8 The appropriate amount is cells / mL. If the inoculation amount exceeds the above range, the fermentation time remains largely unchanged. Conversely, if it falls below the above range, it takes a long time for fermentation to complete, which is undesirable.

[0033] The fermentation temperature is generally in the range of 5 to 50°C, preferably in the range of 20 to 40°C, which is the optimal growth temperature for each microorganism (for example, 30 to 40°C for lactic acid bacteria and 25 to 30°C for yeast). The fermentation period is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimal temperature. If the fermentation period is shorter than the above general range, fermentation will not occur sufficiently and the effectiveness of the fermented product tends to decrease. On the other hand, if it is extended beyond 10 days, not only will no further increase in effectiveness not be observed, but discoloration and an increase in fermentation odor will occur, both of which are undesirable.

[0034] The extract or fermented product prepared as described above may be used as is after adjusting the pH to 3-9, or it may be used at the desired concentration by vacuum concentration or other methods. It may also be dried by conventional methods such as spray drying.

[0035] Furthermore, the extract or fermented product prepared as described above may be refrigerated for a certain period of time to improve storage stability, etc., before using the supernatant.

[0036] The extract or fermented product according to the present invention can be incorporated into topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals). Examples of topical skin preparations include, but are not limited to, emulsions, creams, lotions, essences, packs, masks, lipsticks, foundations, liquid foundations, makeup pressed powders, blushes, face powders, facial cleansers, body shampoos, shampoos for the scalp and hair, hair conditioners, shampoos or tonics for hair growth and nourishment, cleansing cosmetics such as soaps, and bath additives.

[0037] The amount of extract or fermented product according to the present invention can be appropriately adjusted depending on the formulation in which it is formulated. For example, in the case of skincare cosmetics, the amount is in the range of 0.002 to 1.0% by weight (solids by weight, the same applies hereinafter), in the case of makeup cosmetics, the amount is in the range of 0.002 to 1.0% by weight, and in the case of cleansing cosmetics, the amount is in the range of 0.002 to 10.0% by weight. In the case of hair cosmetics, the amount is in the range of 0.0001 to 5.0% by weight as the solid content of the composition.

[0038] When incorporating the extract or fermented product according to the present invention into a topical skin preparation, in addition to the essential components of the above composition, components commonly used in topical skin preparations, such as oily components, surfactants (synthetic and natural), moisturizers, anti-inflammatory agents, thickeners, preservatives / bactericides, anti-acne agents, cell activators, powder components, UV absorbers, antioxidants, whitening agents, pigments, fragrances, etc., can be appropriately added as needed.

[0039] Here, oily components include, for example, plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, chamomile oil, etc.; vitamin A oil; animal-derived oils and fats such as mink oil, turtle oil, etc.; waxes such as beeswax, carnauba wax, rice wax, lanolin, etc.; liquid paraffin, petrolatum, paraffin wax. Examples include hydrocarbons such as custard and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, and higher fatty acid octyldodecyl (octyldodecyl stearate, etc.).

[0040] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, and polyoxyethylene Anionic surfactants such as lenalkylphenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammonium betaine, N,N,N-trialkyl-N-alkyleneammonium carboxybetaine, and N-acylamidopropyl-N′,N′-dimethyl-N′-β-hydroxypropylammonium sulfobetaine can be used.

[0041] As emulsifiers and / or emulsifying aids, stevia derivatives such as enzyme-treated stevia, saponins or their derivatives, casein or its salts (sodium, etc.), sugar-protein complexes, sucrose or its esters, lactose, water-soluble polysaccharides derived from soybeans, complexes of soybean-derived proteins and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (such as enzyme-treated stevia), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their derivatives, lecithin and its derivatives (such as hydrogenated lecithin), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, grains, etc.), etc. may also be included.

[0042] Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid ferment filtrate, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extracts, estradiol, various amino acids and their derivatives.

[0043] Examples of thickening agents include components derived from brown, green, or red algae such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin, pullulan, and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, and hydroxypropylcellulose; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, polyacrylic acid, etc.

[0044] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or its derivatives.

[0045] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and aluminum bromide. These include chloroisoquinolinium, resorcinol, jamal (imidazodinylurea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, tree bark distillates, radish ferment filtrate, plant-derived ethanol such as sugarcane and corn, or 1,3-butylene glycol.

[0046] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0047] Examples of anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer 201, and pyridoxine dicaprylate.

[0048] Examples of powder components include sericite, titanium dioxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powders, grain powders (rice, wheat, corn, millet, etc.), and legume powders (soybeans, adzuki beans, etc.).

[0049] Examples of UV absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0050] Examples of antioxidants include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, and astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), and vitamin A or its derivatives (e.g., retinol palmitate).

[0051] Furthermore, examples of whitening agents include one or more selected from kojic acid or its derivatives, ascorbic acid or its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, resorcinol derivatives, potassium 4-methoxysalicylate, vitamin E or its derivatives, nicotinic acid or its derivatives, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), placental extract (placenta extract), and linoleic acid.

[0052] Examples of kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, as well as kojic acid ethers and kojic acid sugar derivatives such as kojic acid glucoside. Examples of ascorbic acid derivatives include L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid-2- Ascorbic acid ester salts such as magnesium sulfate, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid) and L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid), 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups include hexanoyl, octanoyl, and decanoyl groups), L-ascorbic acid tetraisopalmitate, L- Examples of hydroquinone derivatives include L-ascorbic acid tetra fatty acid esters such as ascorbic acid tetralaurate, 3-O-ethyl ascorbic acid, and L-ascorbic acid-2-phosphate-6-O-palmitate sodium. Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate; examples of vitamin E derivatives include vitamin E nicotinate and vitamin E linoleate; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0053] Furthermore, the following seaweed, plant, microorganism, or other biologically derived ingredients can also be used in combination. For example, collagen or its hydrolysates, yeast extract or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, camellias, roses, peony, citrus plants, amaranthaceae plants, eelgrasses, legumes, daisies, legumes, mallows, gentians, mints, lotus, Araliaceae, nightshades, bignoniaceae, Actinidiaceae, mulberries, irises, bellflowers, olives, Actinidiaceae, mulberries, rhamnoides, orchids, sumac, and Garcinia. Examples include extracts or hydrolyzed or fermented products thereof from one or more plants selected from the Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Actinidiaceae, Convolvulaceae, and Asparagaceae families; extracts or hydrolyzed or fermented products thereof from one or more seaweeds selected from the Laminariaceae, Mylinaceae, and Ulvaceae families; jellyfish (autolyzed products of moon jellyfish, Echizen jellyfish, etc.); hydrolyzed or fermented products of hyaluronic acid; and extracts or hydrolyzed or fermented products thereof of royal jelly.

[0054] As plant-derived components from the grass family, particularly preferred are hydrolyzed rice extract, hydrolyzed rice bran extract, hydrolyzed germinated brown rice, fermented rice liquid, sake lees extract derived from sake, and bamboo shoot peel extract of Madake or Moso bamboo. As plant-derived components from the Brassicaceae family, particularly preferred are extracts of the seeds of Hakugai, Ougai, or Kokugai, or their hydrolysates or ferments. As plant-derived components from the Camellia family, particularly preferred are green tea and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey Black Tea, etc.). As plant-derived components from the Rosaceae family, preferred are extracts of peach blossoms, leaves, or immature fruit, apricot fruit or seed extract, strawberry flower extract, Damask rose or wild rose flower extract, and cherry blossom or leaf extract. As plant-derived components from the Paeoniaceae family, preferred are extracts of peony root or flower, and peony flower or root. As plant-derived components from the Amaranthaceae family, particularly preferred is Salicornia europaea extract. Furthermore, as a component derived from Zosteraceae plants, extracts of Zostera marina or Zostera japonica are particularly preferred. As a component derived from Fabaceae plants, extracts of white soybeans or black soybeans or their hydrolysates or fermented soy milk liquid, adzuki bean extract, red clover extract, and kudzu root extract are particularly preferred. Furthermore, as a component derived from Fabaceae plants, rooibos extract is preferred. Furthermore, as a component derived from Asteraceae plants, burdock root extract, starflower extract, sunflower extract, arnica extract, or chamomile flower extract are particularly preferred. As a component derived from Malvaceae plants, extracts or fermented products of hibiscus, rose of Sharon, or hibiscus are preferred. As a component derived from Gentianaceae plants, gentian extract is preferred. Furthermore, as a component derived from Lamiaceae plants, green perilla extract is preferred. As a component derived from Nelumbonaceae plants, extracts of lotus flowers or lotus seeds or fermented lotus seeds are particularly preferred. As a component derived from Araliaceae plants, extracts or fermented products of ginseng are preferred. Examples of ingredients derived from Solanaceae plants include extracts of eggplant (long eggplant, water eggplant, American eggplant, Kamo eggplant, etc.). Pau d'arco bark extract is preferred as an ingredient derived from Bignoniaceae plants. Moraceae plant extracts include mulberry bark extract, mulberry fruit extract, and fig fruit or bark extract. Ziziphus jujuba fruit extract is preferred as an ingredient derived from Rhamnaceae plants. Saffron is preferred as an ingredient derived from Iridaceae plants.As a component derived from plants of the Campanulaceae family, extract or hydrolysate of the root of Codonopsis lanceolata is preferred. As a component derived from plants of the Anacardiaceae family, mango fruit extract is particularly preferred. As a component derived from plants of the Garciniaaceae family, mangosteen fruit extract is particularly preferred. Also, as a component derived from plants of the Valenciaceae family, cherimoya fruit extract is preferred. As a component derived from plants of the Rutaceae family, extracts of Satsuma mandarin, bergamot fruit, grapefruit or pomelo fruit (including immature fruit), extracts containing flavonoids and their glycosides found in plants such as grapefruit or hassaku, or sansho seed extract are preferred. As a component derived from plants of the Liliaceae family, extracts of Casablanca lily, Madonna lily, or Sasayuri lily are preferred. As a component derived from plants of the Crassulaceae family, extract or fermented product of Rhodiola rosea is particularly preferred. As a component derived from plants of the Oleaceae family, jasmine flower extract is particularly preferred. As for plants of the Cupressaceae family, juniper fruit extract is particularly preferred. As for components derived from plants of the Myrtaceae family, guava leaf extract is particularly preferred. As for plants of the Orchidaceae family, extract of the root (white) of Bletilla striata is particularly preferred. As for components derived from plants of the Convolvulaceae family, sweet potato extract or its fermented product, or sweet potato shochu lees extract or its fermented product is preferred. As for plants of the Asparagaceae family, asparagus (green asparagus and white asparagus) is preferred. As for components derived from plants of the Actinidiaceae family, kiwi extract is preferred. As for components derived from seaweed of the Laminariaceae family, kelp extract is particularly preferred, as for seaweed of the Mylinaceae family, sorghum extract is preferred, and as for seaweed of the Ulvaceae family, Ulva extract is particularly preferred. As for components derived from seaweed of the Seaweedaceae family, Seaweed extract is particularly preferred.

[0055] The following describes examples of plant-derived components that are the active ingredients of the present invention, but the present invention is not limited thereto. Preparation Example 1: Preparation of Callicarpa japonica extract 20 g of the fruit of Callicarpa japonica was added to 200 g of a mixture of purified water and 1,3-butylene glycol (1:1 ratio), and extracted at 40°C for 2 hours. The resulting solution was filtered to obtain 158 g of a brown, transparent solution (solid content concentration 1.50%).

[0056] Preparation Example 2: Preparation of Tabebuia Extract 100 g of finely chopped inner bark of Tabebuia impetiginosa was mixed with 1000 g of purified water, extracted at 4°C for 24 hours, and then filtered to obtain 650 g of a pale brown, transparent extract solution (solid content concentration 1.48%).

[0057] Production Example 3. Preparation of fermented licorice product An extract suspension was prepared by adding 1500g of finely chopped Hemerocallis fulva flowers to 1500g of purified water and then heat-sterilizing it. Yeast (Saccharomyces cerevisiae) was added to this extract suspension for 10 minutes. 8 The culture was inoculated at a dose of 1 / mL and incubated under a nitrogen stream at 30°C for 3 days. After incubation, the culture solution was heat-sterilized, filtered, deodorized, and decolorized to obtain 1105 g of a brown, transparent yeast fermentation solution (solid content 3.90%).

[0058] Production Example 4. Preparation of Fermented Hemerocallis fulva Product Except for using Hemerocallis middendorffii instead of Hemerocallis fulva in Production Example 3, the procedure was the same as in Production Example 1 to obtain 1160 g of lactic acid bacteria fermented product solution (solid content concentration 4.0%).

[0059] Manufacturing Example 5. Preparation of Fermented Job's Tears Product 50g of hulled Job's tears seeds were crushed, and 950g of purified water was added to prepare a suspension, which was then heat-sterilized. Yeast (Saccharomyces cerevisiae) was added to this liquid for 10 minutes. 7 The sample was inoculated at 1 / mL and incubated at 30°C for 3 days. After incubation, it was heat-sterilized, cooled to room temperature, and filtered to obtain 525 g of adlay seed ferment solution (solid content concentration 1.42%).

[0060] Manufacturing Example 6. Loofah Extract 200g of fresh loofah fruit was cut and juiced, and the resulting solution was heated at 40°C for 1 hour. After heating, it was filtered to obtain 120g (solids concentration 4.50%). This was diluted 2-fold with purified water to obtain loofah extract solution.

[0061] Test Example 1. Evaluation test of the effect of enhancing ceramide synthase activity. Human epidermal cells (NHEK) were placed in 4 × 10⁶ wells of a 96-well microplate containing HuMedia KG2 medium (Kurabo Industries Ltd.). 3Seeds were seeded in individual holes and pre-cultured for 1 day under conditions of 37°C and 5.0% CO2. Then, HuMedia KG2 medium containing an extract or ferment from one of the production examples 1-6 as a sample solution was added, and the cells were cultured for a further 2 days under the same conditions. Here, the concentrations of each sample solution were adjusted so that the final solution concentrations relative to the volume of medium were 1.0% and 2.0% for the extracts from production examples 1 and 2, and 2.0% and 2.0% for the fermentations from production examples 3-5, respectively. For the extract from production example 6, the final solution concentrations relative to the volume of medium were adjusted to 2.5% and 5.0%. Next, the medium was removed, and 20 μL of PBS(-) solution containing 1 mM Phenylmethyl sulfonyl fluoride (PMSF) and 1% Triton-X was added. The cells were then allowed to stand at room temperature for 5 minutes to disrupt them and obtain a crude enzyme solution. 1 mM 4-methylumbelliferyl-β-Glucopyranoside, 10 mM sodium taurocholate, 0.1% Triton-Xin, and 20 μL of 0.1 M citrate phosphate buffer (pH 5.6) were added and the mixture was reacted at 37°C for 1 hour. After the reaction was complete, 200 μL of 0.2 M carbonate bicarbonate buffer (pH 10.5) was added to stop the reaction. Subsequently, the fluorescence intensity of the reaction solution in Ex355 / Em460 was measured and used as the β-glucocerebrosidase activity value. In addition, another microplate cultured under the exact same conditions as above was washed once with PBS(-) after the culture was complete, and 100 μL of hoechst33342 reagent diluted 100-fold with PBS(-) was added per well. The mixture was incubated at 37°C for 1 hour and the DNA was fluorescently stained. Subsequently, the fluorescence intensity [excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, Thermo Fisher Scientific)] was measured to determine the amount of DNA. By dividing the obtained β-glucocerebrosidase activity value by the amount of DNA, the β-glucocerebrosidase activity value per unit of DNA in epidermal cells was calculated.Furthermore, the same procedure was performed for the control group (no sample added), where PBS(-) was added instead of the sample solution. The relative value of the β-glucocerebrosidase activity per DNA in each sample-added group was calculated relative to the β-glucocerebrosidase activity per DNA (100) obtained in this case, and this value was defined as the β-glucocerebrosidase activity rate per DNA (%). In addition, to confirm that the test system was functioning correctly, the same test was performed when 0.005% galactocerebroside (GalCer) was added as a positive control instead of the sample solution.

[0062] The results of Test Example 1 are shown in Table 1. [Table 1] JPEG0007881150000001.jpg103134

[0063] As shown in Table 1, the extracts or fermented products of Production Examples 1 to 6 according to the present invention were confirmed to have a significant effect in enhancing the activity of ceramide synthase (β-glucocerebrosidase). This promotes the synthesis of ceramide in the skin, thereby improving the skin's barrier function and preventing skin problems caused by dryness and external irritants.

[0064] Prescription example 1. Lotion [Ingredients] Part Extract from Production Example 1: 1.0 Squalane 0.2 Polyoxyethylene (5.5) cetyl alcohol 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Isopropylmethylphenol 0.1 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Purified water, in an amount that makes the total volume 100 parts

[0065] Prescription example 2. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 1.0 part of the extract from Production Example 2 was used instead of the extract from Production Example 1 contained in Formulation Example 1.

[0066] Prescription example 3. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 1.0 part of the fermented product from Production Example 3 was used instead of the extract from Production Example 1 contained in Formulation Example 1.

[0067] Prescription example 4. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 1.0 part of the fermented product from Production Example 4 was used instead of the extract from Production Example 1 contained in Formulation Example 1.

[0068] Prescription example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 1.0 part of the fermented product from Production Example 5 was used instead of the extract from Production Example 1 contained in Formulation Example 1.

[0069] Prescription example 6. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 1.0 part of the extract from Production Example 6 was used instead of the extract from Production Example 1 contained in Formulation Example 1.

[0070] Prescription example 7. Lotion [Ingredients] Part Extract from Production Example 1 2.0 Glyceryl caprylate 3.0 Polyglyceryl-10 laurate 3.0 Cetanol 2.0 Behenyl alcohol 2.0 Methylparaben 0.1 Ascorbic acid 3.0 Glycyrrhizic acid 0.5 β-Glycyrrhetinic acid 0.05 Tocopherol nicotinate 0.1 Resorcinol 0.1 Zinc oxide 2.0 dl-camphor 0.5 Glycerin 2.0 1,3-Butylene glycol 5.0 Potassium hydroxide 0.5 Purified water, in an amount that makes the total volume 100 parts

[0071] Prescription example 8. Lotion A lotion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of the extract from Production Example 2 were used instead of the extract from Production Example 1 included in Formulation Example 7.

[0072] Prescription example 9. Lotion A lotion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of the fermented product from Production Example 3 were used instead of the extract from Production Example 1 contained in Formulation Example 7.

[0073] Prescription example 10. Lotion A lotion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of the fermented product from Production Example 4 were used instead of the extract from Production Example 1 contained in Formulation Example 7.

[0074] Prescription Example 11: Lotion A lotion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of the fermented product from Production Example 5 were used instead of the extract from Production Example 1 contained in Formulation Example 7.

[0075] Prescription example 12. Lotion A lotion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of the extract from Production Example 6 were used instead of the extract from Production Example 1 included in Formulation Example 7.

[0076] Prescription example 13. Lotion [Ingredients] Part Extract from manufacturing example 1: 0.5 Jojoba oil 1.0 Polyoxyethylene (5.5) cetyl alcohol 5.0 Methylparaben 0.1 Ascorbic acid glucoside 2.0 Tranexamic acid 1.0 Niacinamide 5.0 ε-aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Pyridoxine hydrochloride 0.5 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-camphor 0.1 Purified water, in an amount that makes the total volume 100 parts

[0077] Prescription example 14. Emulsion [Ingredients] Part Extract from Production Example 2 1.0 Squalane 5.0 Cyclopentasiloxane 1.0 Hexaran 3.0 Hexyldecyl isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 laurate 5.0 Polyglyceryl-10 isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Magnesium ascorbic acid phosphate salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed collagen 1.0 Acetylated hyaluronic acid 0.1 Purified water, in an amount that makes the total volume 100 parts

[0078] Prescription example 15. Emulsion An emulsion was obtained in the same manner as in Formula Example 14, except that 2.0 parts of L-ascorbic acid-2-glucoside were used instead of 2.0 parts of magnesium ascorbic acid phosphate in the ingredients of Formula Example 14.

[0079] Prescription example 16. Emulsion An emulsion was obtained in the same manner as in Formula Example 14, except that 1.0 part of tranexamic acid was used instead of 2.0 parts of magnesium ascorbic acid phosphate and 0.5 parts of potassium hydroxide.

[0080] Prescription example 17. Emulsion An emulsion was obtained in the same manner as in Formula Example 14, except that 3.0 parts of 3-O-ethyl ascorbic acid were used instead of 2.0 parts of magnesium ascorbic acid phosphate in the ingredients of Formula Example 14.

[0081] Prescription example 18. Emulsion An emulsion was obtained in the same manner as in Formula Example 14, except that 5.0 parts of niacinamide were used instead of 2.0 parts of magnesium ascorbic acid phosphate in Formula Example 14.

[0082] Prescription example 19. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba wax 1.0 Behenyl alcohol 1.0 Stearyl alcohol 1.0 Candelilla wax 1.0 Lactic acid fermented rice 2.0 Soy-derived hydrogenated lecithin 0.5 Extract from Production Example 1: 1.0 Carboxyvinyl polymer 1.0 Sodium alginate 1.0 Glycerin 4.0 pH adjuster (appropriate amount) Preservative 5.2 Purified water, in an amount that makes the total volume 100 parts

[0083] Prescription example 20. Cream The cream was obtained in the same manner as in Formulation Example 19, except that 1.0 part of the extract from Manufacturing Example 2 was used instead of the extract from Manufacturing Example 1 included in Formulation Example 19.

[0084] Prescription example 21. Cream [Ingredients] Part Extract from Production Example 2 1.0 Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl isostearate 5.0 Lauroyl glutamate di(octyldodecyl / phytosteryl) (Behenir) 5.0 Glyceryl caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-oryzanol 0.1 Behenyl alcohol 2.0 Palmitic acid 2.5 D-Panthenyl alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Niacinamide 5.0 Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Hydrolyzed collagen 1.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-camphor 0.3 l-menthol 0.5 Purified water, in an amount that makes the total volume 100 parts

[0085] Prescription example 22. Cream The cream was obtained in the same manner as in Formula Example 21, except that 1.0 part of the fermented product from Production Example 3 was used instead of the extract from Production Example 2 included in Formula Example 21.

[0086] Prescription example 23. Cream The cream was obtained in the same manner as in Formula Example 21, except that 1.0 part of the fermented product from Production Example 4 was used instead of the extract from Production Example 1 contained in Formula Example 21.

[0087] Prescription example 24. Cream The cream was obtained in the same manner as in Formulation Example 21, except that 1.0 part of the extract from Production Example 6 was used instead of the extract from Production Example 1 contained in Formulation Example 21.

[0088] Example 26. Pack [Ingredients] Part Extract from Production Example 1 2.0 Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Water-soluble collagen 1.0 Xanthan gum 2.0 Polyglyceryl-6 myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated lecithin 3.0 Hydroxylated lecithin 3.0 Purified water, in an amount that makes the total volume 100 parts

[0089] Prescription Example 27: Hair Shampoo [Ingredients] Part Extract from Production Example 2 2.0 Sodium laureth sulfate 10.0 Glyceryl monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl alcohol 2.0 Behenyl alcohol 2.0 Dimethicone 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene glycol 2.0 Purified water, in an amount that makes the total volume 100 parts

[0090] Example 28. Hair Conditioner [Ingredients] Part Extract from manufacturing example 6 2.0 Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water, in an amount that makes the total volume 100 parts

[0091] Prescription Example 29. Cleansing Cosmetics [Ingredients] Part Fermented product from manufacturing example 3: 2.0 Potassium cocoyl glycine 5.0 Glycerin 10.0 Glyceryl caprylate 1.0 Sodium lauroyl aspartate 10.0 Water-soluble collagen 5.0 Cetanol 3.0 Myristyl alcohol 3.0 Isopropylmethyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated benzalkonium chloride solution 50 0.2 Benzalkonium chloride 0.1 Purified water, in an amount that makes the total volume 100 parts

[0092] Prescription example 30: Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part Fermented product from manufacturing example 4: 2.0 Glycerin 3.0 1,3-Butylene glycol 2.0 L-ascorbic acid 2-glucoside 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Potassium hydroxide (appropriate amount) Purified water, in an amount that makes the total volume 100 parts

[0093] Prescription Example 31: Serum [Ingredients] Part Fermented product from manufacturing example 5: 2.0 Sodium hyaluronate 1.0 Water-soluble collagen 1.0 Tranexamic acid 0.1 Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Citric acid 0.3 Sodium citrate 0.6 Katamenkirinsai extract 5.0 Purified water, in an amount that makes the total volume 100 parts

Claims

[Claim 1] A β-glucocerebrosidase activity enhancer containing an extract of the fruit of Callicarpa japonica as an active ingredient.

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