Esophageal cancer detection kit or device and detection method

Abstract

To provide a kit or a device for the detection of esophageal cancer and a method for detecting esophageal cancer.SOLUTION: The present invention provides a kit or a device for the detection of esophageal cancer, comprising nucleic acid(s) capable of specifically binding to miRNA(s) in a sample of a subject, and a method for detecting esophageal cancer, comprising measuring the miRNA in vitro.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a kit or device for detecting esophageal cancer, which contains a nucleic acid that can specifically bind to a specific miRNA and is used for examining the presence or absence of esophageal cancer in a subject, and a method for detecting esophageal cancer, which includes measuring the expression level of the miRNA using the nucleic acid.

Background Art

[0002] The esophagus is a tubular organ that transports food from the mouth to the stomach and is located between the trachea and the spine. The wall of the esophagus is divided into four layers from the inside to the outside: the mucosa, submucosa, muscularis propria, and adventitia, each of which is responsible for the function of transporting food from the mouth to the stomach (Non-Patent Document 1). According to the statistics of cancers by site in Japan in 2012 disclosed by the Cancer Countermeasure Information Center of the National Cancer Center, National Institute of Biomedical Innovation, the number of deaths due to esophageal cancer was 11,592, ranking 10th among cancer death causes by site. Also, the risk of death from esophageal cancer in Japan is 5.6 times higher in men than in women, and smoking and alcohol intake are regarded as risk factors for esophageal cancer (Non-Patent Document 1). In the United States, it is said that esophageal cancer develops in 1 out of 125 men and 1 out of 435 women, and the estimated number of esophageal cancer patients in 2014 is as high as 18,170, and about 15,450 of them are said to die (Non-Patent Document 1 ). The progression of esophageal cancer is defined in Non-Patent Document 2, and is staged by the size of the tumor (Tis, T1-3 and T4a-b), lymph node metastasis (N1-3), distant metastasis (M0-1), etc.

[0003] ​​​​​​Stage 0 (Tis / N0 / M0), Stage IA (T1 / N0 / M0), Stage IB (T Stage 2 / N0 / M0), Stage IIA (T3 / N0 / M0), Stage IIB (T1~2 / Stage IIIA (T4a / N0 / M0, T3 / N1 / M0, and T1 ~2 / N2 / M0), Stage IIIB (T3 / N2 / M0), Stage IIIC (T4 a / N1~2 / M0, T4b / M0, and N3 / M0), and Stage IV (M1) It is classified as follows:

[0004] The 5-year relative survival rate for esophageal cancer is highly dependent on the stage of cancer progression, and is limited to cancers confined to esophageal tissue. In contrast, the figures are 39% for cancers confined to adjacent tissues, 21% for cancers that have metastasized to distant tissues, and 4% for cancers that have metastasized to distant tissues. This has been reported (Non-Patent Document 1). Therefore, early detection of esophageal cancer is important for survival rates. Providing means to enable early detection is strongly desired, as it will lead to a significant improvement in this area.

[0005] Treatment for esophageal cancer is determined by the stage of the cancer and the patient's overall health, and mainly involves endoscopic treatment, surgery, and There are radiation therapy and chemotherapy. For cancers that have progressed to a certain extent, these treatments are combined. Multidisciplinary treatment is performed to create synergistic effects while making the most of the characteristics of each component (Non-patent Literature) 1). In addition, for early-stage esophageal cancer such as stage 0 or 1, endoscopic treatment is less burdensome for the patient. In some cases, photodynamic therapy may be applicable (Non-Patent Document 1).

[0006] According to Non-Patent Document 1, common examinations for esophageal cancer include X-ray esophagography and endoscopy. This is an examination. In addition, CT scans, MRI scans, and endoscopic ultrasound scans are performed to check the extent of the cancer's spread. Tests such as ultrasound are performed. If these primary tests reveal findings suggestive of esophageal cancer, As a secondary examination, a pathological examination is performed in which cells or tissues are collected by inserting a needle into the lesion and examined under a microscope. In addition, blood tumor markers for detecting esophageal cancer include, for example, CEA and SCC. This is known (Non-Patent Document 3).

[0007] Although still in the research stage, as shown in Patent Document 1, biological samples including blood The expression level of microRNAs (miRNAs) within the protein, or the relationship between the expression level of miRNAs and other proteins. There are reports that esophageal cancer can be detected by combining it with the expression levels of markers. .

[0008] Patent Document 1 describes miR-663a, miR-92a-3p, and miR-575 in serum. A method for detecting esophageal cancer by measuring miRNAs such as these has been described. [Prior art documents] [Patent Documents]

[0009] [Patent Document 1] U.S. Patent Application Publication No. 2014 / 031246 [Non-patent literature]

[0010] [Non-Patent Document 1] American Cancer Society, "Esophagus Cancer," 2014 (supervised), pp. 2-8, 19-20, 29-41. [Non-Patent Document 2] Sobin, L. et al., “TNM Classification of Malignant Tumors 7th Edition Japanese Version” 2009, p.63-68 [Non-Patent Document 3] Terada, T. et al., 2013, International Journal of Clinical and Experimental Medicine, Vol. 6(3), pp. 219-26

Summary of the Invention

Problems to be Solved by the Invention

[0011] The problem of the present invention is to find a novel esophageal cancer tumor marker and provide a method capable of effectively detecting esophageal cancer using a nucleic acid that can specifically bind to the marker. As described in Non-Patent Document 1, general examinations for esophageal cancer are barium swallow

[0012] examination by X-ray and endoscopic examination. However, in ordinary medical check-ups, emphasis is placed on the examination of gastric cancer, and the esophagus is often not sufficiently observed. Also, even today when these examinations have become widespread, the number of deaths due to esophageal cancer in this country is on the rise, and it cannot be said that image diagnosis has necessarily suppressed esophageal cancer. In addition, although CT examination and MRI examination can detect esophageal cancer with high performance, special equipment is required and the examination cost is high, so it is not suitable for wide spread as a primary examination.

[0013] Blood tumor markers for the detection of esophageal cancer, such as CEA and SCC, are known (Non-Patent Document 3), but these markers have problems in terms of accuracy, such as increasing in cancers other than esophageal cancer, so their usefulness has not been established. If these markers are used and another cancer is misdiagnosed as esophageal cancer, the opportunity for appropriate treatment may be missed, or incorrect medical treatment may be applied, imposing unnecessary economic and physical burdens on the patient. Therefore

[0014]

[0015] The American Cancer Society's guidelines for esophageal cancer also do not mention these markers (non-patent document). 1).

[0014] Furthermore, although still in the research stage, microRNAs (miR) in biological samples, including blood, are being investigated. There are reports, as described below, that use the expression level of NA to identify esophageal cancer, but none of them It has not yet been put into practical use.

[0015] Patent Document 1 describes miR-663a, miR-92a-3p, and miR-575 in serum. A method for detecting esophageal cancer by measuring miRNAs such as those described above has been presented. Specifically, in healthy individuals... A list of miRNAs that fluctuate in the serum of 16 esophageal cancer patients is shown, compared to 12 individuals. The expression level of that miRNA is measured to determine whether or not it is esophageal cancer. However, this test The method describes the specific accuracy, sensitivity, and specificity of detection performance for identifying esophageal cancer. Furthermore, there is little information available, and its industrial practicality is limited. The only verified hsa-miR- There is a note that 345 has an AUC of 0.814, making it difficult to distinguish esophageal cancer from it alone.

[0016] Thus, in detecting esophageal cancer, existing tumor markers have low performance, or Since the performance and detection methods of markers in the research stage have not been specifically described, these Using this method could lead to unnecessary additional tests due to the misidentification of healthy individuals as esophageal cancer patients. Furthermore, there is a possibility of missing treatment opportunities due to overlooking esophageal cancer patients. Measuring miRNAs, which consist of tens to hundreds of molecules, increases the cost of testing, therefore, health checkups It is difficult to use for large-scale screenings such as diagnostic tests. Furthermore, it is not suitable for measuring tumor markers. Since taking esophageal tissue is highly invasive and undesirable for patients, a less invasive method is preferred. It is possible to detect it from blood samples, and to identify esophageal cancer patients as esophageal cancer patients, There is a need for highly accurate esophageal cancer markers that can correctly distinguish healthy individuals from healthy ones. In particular, early detection and treatment of esophageal cancer can significantly improve survival rates. In terms of treatment options, less burdensome endoscopic treatments and photodynamic therapies have become available. Therefore, there is a strong need for a highly sensitive esophageal cancer marker that can detect even early-stage esophageal cancer. It is being done. [Means for solving the problem]

[0017] In order to solve the above problems, the inventors have diligently studied and found a detection marker for esophageal cancer from blood. By identifying several genes that can be used and using nucleic acids that can specifically bind to them, We discovered that esophageal cancer can be detected significantly, and thus completed the present invention. <Summary of the Invention> In other words, the present invention has the following features. (1) Esophageal cancer markers, miR-204-3p, miR-1247-3p, mi R-6875-5p, miR-6857-5p, miR-6726-5p, miR-31 88, miR-8069, miR-4257, miR-1343-3p, miR-710 8-5p, miR-6825-5p, miR-7641, miR-3185, miR-4 746-3p, miR-6791-5p, miR-6893-5p, miR-4433b -3p, miR-3135b, miR-6781-5p, miR-1908-5p, mi R-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150 -3p, miR-4433-3p, miR-6125, miR-4513, miR-67 87-5p, miR-6784-5p, miR-615-5p, miR-6765-3p miR-5572 miR-6842-5p miR-8063 miR-6780b -5p、miR-187-5p、miR-128-1-5p、miR-6729-5p、 miR-6741-5p, miR-6757-5p, miR-7110-5p, miR- 7975, miR-1233-5p, miR-6845-5p, miR-3937, mi R-4467, miR-7109-5p, miR-6088, miR-6782-5p miR-5195-3p, miR-4454, miR-6724-5p, miR-807 2, miR-4516, miR-6756-5p, miR-4665-3p, miR-6 826-5p, miR-6820-5p, miR-6887-5p, miR-3679- 5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p miR-939-5p, miR-602, miR-7977, miR-6749-5p miR-1914-3p, miR-4651, miR-4695-5p, miR-684 8-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p miR-3620-5p miR-3131 miR-6732-5p miR-71 13-3p, miR-23a-3p, miR-3154, miR-4723-5p, mi R-3663-3p, miR-4734, miR-6816-5p, miR-4442 miR-4476, miR-423-5p, miR-1249, miR-6515-3p miR-887-3p miR-4741 miR-6766-3p miR-467 3, miR-6779-5p, miR-4706, miR-1268b, miR-463 2-5p, miR-3197, miR-6798-5p, miR-711, miR-68 40-3p, miR-6763-5p, miR-6727-5p, miR-371a-5 p, miR-6824-5p, miR-4648, miR-1227-5p, miR-5 64、miR-3679-3p、miR-2861、miR-6737-5p、miR- 4725-3p, miR-6716-5p, miR-4675, miR-1915-3p miR-671-5p miR-3656 miR-6722-3p miR-470 7-5p, miR-4449, miR-1202, miR-4649-5p, miR-7 44-5p, miR-642a-3p, miR-451a, miR-6870-5p, m iR-4443, miR-6808-5p, miR-4728-5p, miR-937- 5p, miR-135a-3p, miR-663b, miR-1343-5p, miR- 6822-5p, miR-6803-5p, miR-6805-3p, miR-128- 2-5p, miR-4640-5p, miR-1469, miR-92a-2-5p, m iR-3940-5p, miR-4281, miR-1260b, miR-4758-5 p, miR-1915-5p, miR-5001-5p, miR-4286, miR-6 126, miR-6789-5p, miR-4459, miR-1268a, miR-6 752-5p, miR-6131, miR-6800-5p, miR-4532, miR -6872-3p, miR-718, miR-6769a-5p, miR-4707-3 p, miR-6765-5p, miR-4739, miR-4525, miR-4270 , miR-4534, miR-6785-5p, miR-6850-5p, miR-46 97-5p, miR-1260a, miR-4486, miR-6880-5p, miR -6802-5p, miR-6861-5p, miR-92b-5p, miR-1238 -5p, miR-6851-5p, miR-7704, miR-149-3p, miR- 4689, miR-4688, miR-125a-3p, miR-23b-3p, miR -614, miR-1913, miR-16-5p, miR-6717-5p, miR- 3648, miR-3162-5p, miR-1909-3p, miR-8073, mi R-6769b-5p, miR-6836-3p, miR-4484, miR-6819 At least one polynucleus selected from the group consisting of -5p and miR-6794-5p A kit for detecting esophageal cancer, containing nucleic acids that can specifically bind to rheotide. (2) miR-204-3p is hsa-miR-204-3p, and miR-1247 -3p is hsa-miR-1247-3p, and miR-6875-5p is hsa-m iR-6875-5p and miR-6857-5p are hsa-miR-6857-5 p is, miR-6726-5p is hsa-miR-6726-5p, and miR- 3188 is hsa-miR-3188, and miR-8069 is hsa-miR-80 69, miR-4257 is hsa-miR-4257, miR-1343- 3p is hsa-miR-1343-3p, and miR-7108-5p is hsa-mi R-7108-5p is the same as miR-6825-5p, and hsa-miR-6825-5p is the same as miR-6825-5p. Therefore, miR-7641 is hsa-miR-7641, and miR-3185 is hs a-miR-3185 is and miR-4746-3p is hsa-miR-4746-3 p is, miR-6791-5p is hsa-miR-6791-5p, and miR- 6893-5p is hsa-miR-6893-5p, and miR-4433b-3p is hsa-miR-4433b-3p, and miR-3135b is hsa-miR-31 35b, and miR-6781-5p is hsa-miR-6781-5p, mi R-1908-5p is hsa-miR-1908-5p, and miR-4792 is hs a-miR-4792 and miR-7845-5p is hsa-miR-7845-5 p is, miR-4417 is hsa-miR-4417, and miR-3184-5 p is hsa-miR-3184-5p, and miR-1225-5p is hsa-miR -1225-5p, and miR-1231 is hsa-miR-1231, miR -1225-3p is hsa-miR-1225-3p, and miR-150-3p is h sa-miR-150-3p and miR-4433-3p is hsa-miR-443 3-3p, miR-6125 is hsa-miR-6125, miR-451 3 is hsa-miR-4513, and miR-6787-5p is hsa-miR-67 87-5p, and miR-6784-5p is hsa-miR-6784-5p, miR-615-5p is hsa-miR-615-5p, and miR-6765-3p hsa-miR-6765-3p and miR-5572 is hsa-miR-557 2, and miR-6842-5p is hsa-miR-6842-5p, and miR- 8063 is hsa-miR-8063, and miR-6780b-5p is hsa-mi It is R-6780b-5p, and miR-187-5p is hsa-miR-187-5p. Yes, miR-128-1-5p is hsa-miR-128-1-5p, and miR- 6729-5p is hsa-miR-6729-5p, and miR-6741-5p is h sa-miR-6741-5p is one such example, and miR-6757-5p is the other such example. 57-5p, and miR-7110-5p is hsa-miR-7110-5p, miR-7975 is hsa-miR-7975, and miR-1233-5p is hsa -miR-1233-5p and miR-6845-5p is hsa-miR-6845 -5p, miR-3937 is hsa-miR-3937, and miR-4467 This is hsa-miR-4467, and miR-7109-5p is hsa-miR-710 9-5p, miR-6088 is hsa-miR-6088, and miR-678 2-5p is hsa-miR-6782-5p, and miR-5195-3p is hsa- It is miR-5195-3p, and miR-4454 is hsa-miR-4454. miR-6724-5p is hsa-miR-6724-5p, and miR-8072 is hsa-miR-8072 and miR-4516 is hsa-miR-4516. miR-6756-5p is hsa-miR-6756-5p, and miR-4665 -3p is hsa-miR-4665-3p, and miR-6826-5p is hsa-m iR-6826-5p is the same as miR-6820-5p, and hsa-miR-6820-5 p is, miR-6887-5p is hsa-miR-6887-5p, and miR- 3679-5p is hsa-miR-3679-5p, and miR-7847-3p is h sa-miR-7847-3p is one such example, and miR-6721-5p is the other, hsa-miR-67 21-5p, and miR-3622a-5p is hsa-miR-3622a-5p. Therefore, miR-939-5p is hsa-miR-939-5p, and miR-602 is h sa-miR-602 is hsa-miR-7977, m iR-6749-5p is hsa-miR-6749-5p, and miR-1914-3 p is hsa-miR-1914-3p, and miR-4651 is hsa-miR-46 51, and miR-4695-5p is hsa-miR-4695-5p, miR -6848-5p is hsa-miR-6848-5p, and miR-1228-3p is hsa-miR-1228-3p is hsa-miR-6 42b-3p, and miR-6746-5p is hsa-miR-6746-5p. miR-3620-5p is hsa-miR-3620-5p, and miR-3131 hsa-miR-3131 and miR-6732-5p are hsa-miR-673 2-5p, and miR-7113-3p is hsa-miR-7113-3p, m iR-23a-3p is hsa-miR-23a-3p, and miR-3154 is hsa -miR-3154, and miR-4723-5p is hsa-miR-4723-5p Therefore, miR-3663-3p is hsa-miR-3663-3p, and miR-4 734 is hsa-miR-4734, and miR-6816-5p is hsa-miR- 6816-5p, and miR-4442 is hsa-miR-4442, and miR- 4476 is hsa-miR-4476, and miR-423-5p is hsa-miR- 423-5p, miR-1249 is hsa-miR-1249, miR-6 515-3p is hsa-miR-6515-3p, and miR-887-3p is hsa -miR-887-3p, and miR-4741 is hsa-miR-4741, miR-6766-3p is hsa-miR-6766-3p, and miR-4673 is hsa-miR-4673 is hsa-miR-6779-5p is hsa-miR-6779 -5p, miR-4706 is hsa-miR-4706, miR-1268 b is hsa-miR-1268b, and miR-4632-5p is hsa-miR-4 632-5p, miR-3197 is hsa-miR-3197, miR-6 798-5p is hsa-miR-6798-5p, and miR-711 is hsa-mi It is R-711, and miR-6840-3p is hsa-miR-6840-3p, miR-6763-5p is hsa-miR-6763-5p, and miR-6727- 5p is hsa-miR-6727-5p, and miR-371a-5p is hsa-mi R-371a-5p and miR-6824-5p are hsa-miR-6824-5p Therefore, miR-4648 is hsa-miR-4648, and miR-1227-5p This is hsa-miR-1227-5p, and miR-564 is hsa-miR-564. Yes, miR-3679-3p is hsa-miR-3679-3p, and miR-28 61 is hsa-miR-2861, and miR-6737-5p is hsa-miR-6 737-5p, and miR-4725-3p is hsa-miR-4725-3p. miR-6716-5p is hsa-miR-6716-5p, and miR-4675 hsa-miR-4675 and miR-1915-3p is hsa-miR-191 5-3p, and miR-671-5p is hsa-miR-671-5p, and miR -3656 is hsa-miR-3656, and miR-6722-3p is hsa-mi R-6722-3p and miR-4707-5p are hsa-miR-4707-5p Therefore, miR-4449 is hsa-miR-4449, and miR-1202 is hs a-miR-1202 is and miR-4649-5p is hsa-miR-4649-5 p is, miR-744-5p is hsa-miR-744-5p, and miR-64 2a-3p is hsa-miR-642a-3p, and miR-451a is hsa-mi It is R-451a, and miR-6870-5p is hsa-miR-6870-5p. miR-4443 is hsa-miR-4443, and miR-6808-5p is hs a-miR-6808-5p is the same as miR-4728-5p, and hsa-miR-472 8-5p, and miR-937-5p is hsa-miR-937-5p, and miR -135a-3p is hsa-miR-135a-3p, and miR-663b is hsa -miR-663b and miR-1343-5p are hsa-miR-1343-5p Therefore, miR-6822-5p is hsa-miR-6822-5p, and miR-6 803-5p is hsa-miR-6803-5p, and miR-6805-3p is hs a-miR-6805-3p and miR-128-2-5p is hsa-miR-12 It is 8-2-5p, and miR-4640-5p is hsa-miR-4640-5p. miR-1469 is hsa-miR-1469, and miR-92a-2-5p is h sa-miR-92a-2-5p is hsa-miR-3 940-5p, miR-4281 is hsa-miR-4281, miR-1 260b is hsa-miR-1260b, and miR-4758-5p is hsa-mi R-4758-5p is the same as miR-1915-5p, and hsa-miR-1915-5p is the same as miR-1915-5p. Therefore, miR-5001-5p is hsa-miR-5001-5p, and miR-4 286 is hsa-miR-4286, and miR-6126 is hsa-miR-612 6, and miR-6789-5p is hsa-miR-6789-5p, and miR- 4459 is hsa-miR-4459, and miR-1268a is hsa-miR-1 268a, and miR-6752-5p is hsa-miR-6752-5p, m iR-6131 is hsa-miR-6131, and miR-6800-5p is hsa- It is miR-6800-5p, and miR-4532 is hsa-miR-4532. miR-6872-3p is hsa-miR-6872-3p, and miR-718 is h sa-miR-718 is the same as miR-6769a-5p, and hsa-miR-6769a -5p, and miR-4707-3p is hsa-miR-4707-3p, mi R-6765-5p is hsa-miR-6765-5p, and miR-4739 is hs a-miR-4739, miR-4525 is hsa-miR-4525, m iR-4270 is hsa-miR-4270, and miR-4534 is hsa-miR It is -4534, and miR-6785-5p is hsa-miR-6785-5p, miR-6850-5p is hsa-miR-6850-5p, and miR-4697- 5p is hsa-miR-4697-5p, and miR-1260a is hsa-miR- 1260a, miR-4486 is hsa-miR-4486, miR-68 80-5p is hsa-miR-6880-5p, and miR-6802-5p is hsa -miR-6802-5p, and miR-6861-5p is hsa-miR-6861 -5p, and miR-92b-5p is hsa-miR-92b-5p, and miR- 1238-5p is hsa-miR-1238-5p, and miR-6851-5p is h sa-miR-6851-5p and miR-7704 is hsa-miR-7704 Yes, miR-149-3p is hsa-miR-149-3p, and miR-4689 is hsa-miR-4689, and miR-4688 is hsa-miR-4688. Therefore, miR-125a-3p is hsa-miR-125a-3p, and miR-23b -3p is hsa-miR-23b-3p, and miR-614 is hsa-miR-61 4, miR-1913 is hsa-miR-1913, and miR-16-5p is hsa-miR-16-5p and miR-6717-5p is hsa-miR-671 7-5p, miR-3648 is hsa-miR-3648, miR-316 2-5p is hsa-miR-3162-5p, and miR-1909-3p is hsa- miR-1909-3p, and miR-8073 is hsa-miR-8073. miR-6769b-5p is hsa-miR-6769b-5p, and miR-683 6-3p is hsa-miR-6836-3p, and miR-4484 is hsa-miR It is -4484, and miR-6819-5p is hsa-miR-6819-5p, Furthermore, miR-6794-5p is hsa-miR-6794-5p, as described in (1). The kit. (3) The nucleic acid is a polynucleotide as shown in (a) to (e) below: (a) Represented by one of the sequence numbers 1-115, 117-189, and 666-675 Polynucleotides consisting of a base sequence or a base sequence in which u is t D, its variants, its derivatives, or fragments thereof containing 15 or more consecutive bases, (b) Represented by any of the sequence numbers 1-115, 117-189, and 666-675 Polynucleotides containing a base sequence (c) Represented by any of the sequence numbers 1-115, 117-189, and 666-675 From a base sequence or a base sequence complementary to the base sequence in which u is t Polynucleotides, their variants, their derivatives, or those containing 15 or more consecutive bases Fragments of (d) Represented by one of the sequence numbers 1-115, 117-189, and 666-675 A base sequence that includes a base sequence that is complementary to a base sequence in which u is t. polynucleotides, and (e) With any of the polynucleotides from (a) to (d) above under stringent conditions A hybridized polynucleotide, The kit according to (1) or (2), which is a polynucleotide selected from the group consisting of the following. (4) The kit contains other esophageal cancer markers, miR-575 and miR-24-3 (1) to (3) further include a polynucleotide consisting of p and nucleic acids that can specifically bind to it. The kit is one of the options listed. (5) miR-575 is hsa-miR-575, and miR-24-3p is hsa- The kit described in (4) is the miR-24-3p. (6) The nucleic acid is a polynucleotide as shown in (f) to (j) below: (f) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide consisting of a base sequence t, its variants, its derivatives, or 15 or more The fragment containing consecutive bases, (g) Polynucleotides containing the base sequences represented by SEQ ID NOs. 116 and 676, (h) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide consisting of a base sequence complementary to a base sequence t, its mutant, and its induction. A body, or a fragment thereof containing 15 or more consecutive bases, (i) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide containing a base sequence complementary to the base sequence t, and (j) With any of the polynucleotides in (f) to (i) above under stringent conditions A hybridized polynucleotide, The kit according to (4) or (5), which is a polynucleotide selected from the group consisting of the following. (7) The kit contains other esophageal cancer markers, miR-675-5p, miR-4 86-3p, miR-6777-5p, miR-4497, miR-296-3p, mi R-6738-5p, miR-4731-5p, miR-6889-5p, miR-67 86-5p, miR-92a-3p, miR-4294, miR-4763-3p, mi R-6076, miR-663a, miR-760, miR-4667-5p, miR- 6090, miR-4730, miR-7106-5p, miR-3196, miR-5 698, miR-6087, miR-4665-5p, miR-8059 and miR-6 It specifically binds to at least one polynucleotide selected from the group consisting of 879-5p. A kit according to any one of (1) to (6), further comprising combinatable nucleic acids. (8) miR-675-5p is hsa-miR-675-5p, and miR-486- 3p is hsa-miR-486-3p, and miR-6777-5p is hsa-miR -6777-5p, and miR-4497 is hsa-miR-4497, miR -296-3p is hsa-miR-296-3p, and miR-6738-5p is hs a-miR-6738-5p is the same as miR-4731-5p, and hsa-miR-473 1-5p, and miR-6889-5p is hsa-miR-6889-5p, m iR-6786-5p is hsa-miR-6786-5p, and miR-92a-3p hsa-miR-92a-3p and miR-4294 are hsa-miR-4294 Therefore, miR-4763-3p is hsa-miR-4763-3p, and miR-6 076 is hsa-miR-6076, and miR-663a is hsa-miR-663 a is, miR-760 is hsa-miR-760, and miR-4667-5p is hsa-miR-4667-5p and miR-6090 is hsa-miR-6090 Therefore, miR-4730 is hsa-miR-4730, and miR-7106-5p This is hsa-miR-7106-5p, and miR-3196 is hsa-miR-319 6, miR-5698 is hsa-miR-5698, and miR-6087 is h sa-miR-6087, and miR-4665-5p is hsa-miR-4665- 5p, miR-8059 is hsa-miR-8059, and miR-68 The kit described in (7) is 79-5p, which is hsa-miR-6879-5p. (9) The nucleic acid is a polynucleotide as shown in (k) to (o) below: (k) A base sequence represented by any of sequence numbers 190 to 214 or the said base sequence A polynucleotide consisting of a base sequence in which u is t, its variants, its derivatives, or 1 The fragment containing 5 or more consecutive bases, (l) Polynucleotides containing a base sequence represented by any of sequence numbers 190-214, (m) A base sequence represented by any of sequence numbers 190-214 or the said base sequence A polynucleotide consisting of a base sequence complementary to the base sequence where u is t, and its variants. Its derivative, or a fragment thereof containing 15 or more consecutive bases, (n) A base sequence represented by any of sequence numbers 190 to 214 or the said base sequence A polynucleotide containing a base sequence complementary to the base sequence in which u is t, and (o) With any of the polynucleotides (k) to (n) above under stringent conditions A hybridized polynucleotide, The kit according to (7) or (8), which is a polynucleotide selected from the group consisting of the following. (10) The kit is selected from all esophageal cancer markers described in (1) or (2) At least two polynucleotides that can specifically bind to each of the at least two polynucleotides A kit containing nucleic acids as described in any of (1) to (9). (11) Esophageal cancer markers, miR-204-3p, miR-1247-3p, m iR-6875-5p, miR-6857-5p, miR-6726-5p, miR-3 188, miR-8069, miR-4257, miR-1343-3p, miR-71 08-5p, miR-6825-5p, miR-7641, miR-3185, miR- 4746-3p, miR-6791-5p, miR-6893-5p, miR-4433 b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, m iR-4792, miR-7845-5p, miR-4417, miR-3184-5p , miR-1225-5p, miR-1231, miR-1225-3p, miR-15 0-3p, miR-4433-3p, miR-6125, miR-4513, miR-6 787-5p, miR-6784-5p, miR-615-5p, miR-6765-3 p, miR-5572, miR-6842-5p, miR-8063, miR-6780 b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p , miR-6741-5p, miR-6757-5p, miR-7110-5p, miR -7975, miR-1233-5p, miR-6845-5p, miR-3937, m iR-4467, miR-7109-5p, miR-6088, miR-6782-5p , miR-5195-3p, miR-4454, miR-6724-5p, miR-80 72, miR-4516, miR-6756-5p, miR-4665-3p, miR- 6826-5p, miR-6820-5p, miR-6887-5p, miR-3679 -5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p miR-939-5p miR-602 miR-7977 miR-6749-5p miR-1914-3p miR-4651 miR-4695-5p miR-68 48-5p, miR-1228-3p, miR-642b-3p, miR-6746-5 p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7 113-3p, miR-23a-3p, miR-3154, miR-4723-5p, m iR-3663-3p, miR-4734, miR-6816-5p, miR-4442 miR-4476 miR-423-5p miR-1249 miR-6515-3 p, miR-887-3p, miR-4741, miR-6766-3p, miR-46 73, miR-6779-5p, miR-4706, miR-1268b, miR-46 32-5p, miR-3197, miR-6798-5p, miR-711, miR-6 840-3p, miR-6763-5p, miR-6727-5p, miR-371a- 5p、miR-6824-5p、miR-4648、miR-1227-5p、miR- 564, miR-3679-3p, miR-2861, miR-6737-5p, miR -4725-3p, miR-6716-5p, miR-4675, miR-1915-3 p, miR-671-5p, miR-3656, miR-6722-3p, miR-47 07-5p、miR-4449、miR-1202、miR-4649-5p、miR- 744-5p, miR-642a-3p, miR-451a, miR-6870-5p miR-4443, miR-6808-5p, miR-4728-5p, miR-937 -5p, miR-135a-3p, miR-663b, miR-1343-5p, miR -6822-5p, miR-6803-5p, miR-6805-3p, miR-128 -2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、 miR-3940-5p, miR-4281, miR-1260b, miR-4758- 5p、miR-1915-5p、miR-5001-5p、miR-4286、miR- 6126、miR-6789-5p、miR-4459、miR-1268a、miR- 6752-5p, miR-6131, miR-6800-5p, miR-4532, mi R-6872-3p, miR-718, miR-6769a-5p, miR-4707- 3p, miR-6765-5p, miR-4739, miR-4525, miR-427 0, miR-4534, miR-6785-5p, miR-6850-5p, miR-4 697-5p, miR-1260a, miR-4486, miR-6880-5p, mi R-6802-5p, miR-6861-5p, miR-92b-5p, miR-123 8-5p, miR-6851-5p, miR-7704, miR-149-3p, miR -4689, miR-4688, miR-125a-3p, miR-23b-3p, mi R-614, miR-1913, miR-16-5p, miR-6717-5p, miR -3648, miR-3162-5p, miR-1909-3p, miR-8073, m iR-6769b-5p, miR-6836-3p, miR-4484, miR-681 At least one polyne selected from the group consisting of 9-5p and miR-6794-5p A device for detecting esophageal cancer, containing nucleic acids that can specifically bind to creotides. (12) miR-204-3p is hsa-miR-204-3p, and miR-124 7-3p is hsa-miR-1247-3p, and miR-6875-5p is hsa- miR-6875-5p is miR-6857-5p is hsa-miR-6857- It is 5p, and miR-6726-5p is hsa-miR-6726-5p, and miR -3188 is hsa-miR-3188, and miR-8069 is hsa-miR-8 069, miR-4257 is hsa-miR-4257, and miR-1343 -3p is hsa-miR-1343-3p, and miR-7108-5p is hsa-m iR-7108-5p and miR-6825-5p are hsa-miR-6825-5 p is, miR-7641 is hsa-miR-7641, and miR-3185 is h sa-miR-3185, and miR-4746-3p is hsa-miR-4746- 3p, and miR-6791-5p is hsa-miR-6791-5p, miR -6893-5p is hsa-miR-6893-5p, and miR-4433b-3p hsa-miR-4433b-3p and miR-3135b is hsa-miR-3 135b, and miR-6781-5p is hsa-miR-6781-5p, m iR-1908-5p is hsa-miR-1908-5p, and miR-4792 is h sa-miR-4792, and miR-7845-5p is hsa-miR-7845- 5p, miR-4417 is hsa-miR-4417, miR-3184- 5p is hsa-miR-3184-5p, and miR-1225-5p is hsa-mi It is R-1225-5p, and miR-1231 is hsa-miR-1231, mi R-1225-3p is hsa-miR-1225-3p, and miR-150-3p is hsa-miR-150-3p and miR-4433-3p is hsa-miR-44 33-3p, miR-6125 is hsa-miR-6125, miR-45 13 is hsa-miR-4513, and miR-6787-5p is hsa-miR-6 787-5p, and miR-6784-5p is hsa-miR-6784-5p. miR-615-5p is hsa-miR-615-5p, and miR-6765-3 p is hsa-miR-6765-3p, and miR-5572 is hsa-miR-55 72, and miR-6842-5p is hsa-miR-6842-5p, and miR -8063 is hsa-miR-8063, and miR-6780b-5p is hsa-m iR-6780b-5p and miR-187-5p are hsa-miR-187-5p Therefore, miR-128-1-5p is hsa-miR-128-1-5p, and miR -6729-5p is hsa-miR-6729-5p, and miR-6741-5p is hsa-miR-6741-5p is hsa-miR-6757-5p is hsa-miR-6 757-5p, and miR-7110-5p is hsa-miR-7110-5p. miR-7975 is hsa-miR-7975, and miR-1233-5p is hs a-miR-1233-5p is and miR-6845-5p is hsa-miR-684 5-5p, miR-3937 is hsa-miR-3937, and miR-446 7 is hsa-miR-4467, and miR-7109-5p is hsa-miR-71 09-5p, miR-6088 is hsa-miR-6088, miR-67 82-5p is hsa-miR-6782-5p, and miR-5195-3p is hsa -miR-5195-3p, and miR-4454 is hsa-miR-4454. miR-6724-5p is hsa-miR-6724-5p, and miR-8072 This is hsa-miR-8072, and miR-4516 is hsa-miR-4516. Therefore, miR-6756-5p is hsa-miR-6756-5p, and miR-466 5-3p is hsa-miR-4665-3p, and miR-6826-5p is hsa- miR-6826-5p is the same as miR-6820-5p is the same as hsa-miR-6820- It is 5p, and miR-6887-5p is hsa-miR-6887-5p, and miR -3679-5p is hsa-miR-3679-5p, and miR-7847-3p is hsa-miR-7847-3p is hsa-miR-6 721-5p, and miR-3622a-5p is hsa-miR-3622a-5p. Yes, miR-939-5p is hsa-miR-939-5p, and miR-602 is hsa-miR-602 and miR-7977 are hsa-miR-7977. miR-6749-5p is hsa-miR-6749-5p, and miR-1914- 3p is hsa-miR-1914-3p, and miR-4651 is hsa-miR-4 651, and miR-4695-5p is hsa-miR-4695-5p, mi R-6848-5p is hsa-miR-6848-5p, and miR-1228-3p hsa-miR-1228-3p and miR-642b-3p is hsa-miR- 642b-3p, and miR-6746-5p is hsa-miR-6746-5p. Therefore, miR-3620-5p is hsa-miR-3620-5p, and miR-313 1 is hsa-miR-3131, and miR-6732-5p is hsa-miR-67 32-5p, and miR-7113-3p is hsa-miR-7113-3p, miR-23a-3p is hsa-miR-23a-3p, and miR-3154 is hs a-miR-3154 is and miR-4723-5p is hsa-miR-4723-5 p is, miR-3663-3p is hsa-miR-3663-3p, and miR- 4734 is hsa-miR-4734, and miR-6816-5p is hsa-miR -6816-5p, and miR-4442 is hsa-miR-4442, miR -4476 is hsa-miR-4476, and miR-423-5p is hsa-miR -423-5p, and miR-1249 is hsa-miR-1249, and miR- 6515-3p is hsa-miR-6515-3p, and miR-887-3p is hs a-miR-887-3p and miR-4741 is hsa-miR-4741. miR-6766-3p is hsa-miR-6766-3p, and miR-4673 hsa-miR-4673 and miR-6779-5p is hsa-miR-677 9-5p, miR-4706 is hsa-miR-4706, miR-126 8b is hsa-miR-1268b, and miR-4632-5p is hsa-miR- 4632-5p, and miR-3197 is hsa-miR-3197, and miR- 6798-5p is hsa-miR-6798-5p, and miR-711 is hsa-m iR-711 and miR-6840-3p are hsa-miR-6840-3p miR-6763-5p is hsa-miR-6763-5p, and miR-6727 -5p is hsa-miR-6727-5p, and miR-371a-5p is hsa-m iR-371a-5p and miR-6824-5p are hsa-miR-6824-5 p is, miR-4648 is hsa-miR-4648, and miR-1227-5 p is hsa-miR-1227-5p, and miR-564 is hsa-miR-564. Therefore, miR-3679-3p is hsa-miR-3679-3p, and miR-2 861 is hsa-miR-2861, and miR-6737-5p is hsa-miR- 6737-5p, and miR-4725-3p is hsa-miR-4725-3p. Therefore, miR-6716-5p is hsa-miR-6716-5p, and miR-467 5 is hsa-miR-4675, and miR-1915-3p is hsa-miR-19 15-3p, and miR-671-5p is hsa-miR-671-5p, mi R-3656 is hsa-miR-3656, and miR-6722-3p is hsa-m iR-6722-3p and miR-4707-5p are hsa-miR-4707-5 p is, miR-4449 is hsa-miR-4449, and miR-1202 is h sa-miR-1202, and miR-4649-5p is hsa-miR-4649- It is 5p, and miR-744-5p is hsa-miR-744-5p, and miR-6 42a-3p is hsa-miR-642a-3p, and miR-451a is hsa-m iR-451a and miR-6870-5p are hsa-miR-6870-5p Therefore, miR-4443 is hsa-miR-4443, and miR-6808-5p is h sa-miR-6808-5p is the same as miR-4728-5p, and hsa-miR-47 28-5p is, and miR-937-5p is hsa-miR-937-5p, mi R-135a-3p is hsa-miR-135a-3p, and miR-663b is hs a-miR-663b and miR-1343-5p is hsa-miR-1343-5 p is, miR-6822-5p is hsa-miR-6822-5p, and miR- 6803-5p is hsa-miR-6803-5p, and miR-6805-3p is h sa-miR-6805-3p is hsa-miR-1 28-2-5p, and miR-4640-5p is hsa-miR-4640-5p. Therefore, miR-1469 is hsa-miR-1469, and miR-92a-2-5p is hsa-miR-92a-2-5p and miR-3940-5p is hsa-miR- 3940-5p, miR-4281 is hsa-miR-4281, miR- 1260b is hsa-miR-1260b, and miR-4758-5p is hsa-m iR-4758-5p and miR-1915-5p are hsa-miR-1915-5 p is, miR-5001-5p is hsa-miR-5001-5p, and miR- 4286 is hsa-miR-4286, and miR-6126 is hsa-miR-61 26, and miR-6789-5p is hsa-miR-6789-5p, miR -4459 is hsa-miR-4459, and miR-1268a is hsa-miR- It is 1268a, and miR-6752-5p is hsa-miR-6752-5p, miR-6131 is hsa-miR-6131, and miR-6800-5p is hsa -miR-6800-5p, and miR-4532 is hsa-miR-4532. miR-6872-3p is hsa-miR-6872-3p, and miR-718 is hsa-miR-718 is hsa-miR-6769a-5p is hsa-miR-6769 a-5p, miR-4707-3p is hsa-miR-4707-3p, m iR-6765-5p is hsa-miR-6765-5p, and miR-4739 is h sa-miR-4739 is hsa-miR-4525, and miR-4525 is hsa-miR-4525. miR-4270 is hsa-miR-4270, and miR-4534 is hsa-mi It is R-4534, and miR-6785-5p is hsa-miR-6785-5p. miR-6850-5p is hsa-miR-6850-5p, and miR-4697 -5p is hsa-miR-4697-5p, and miR-1260a is hsa-miR -1260a, miR-4486 is hsa-miR-4486, miR-6 880-5p is hsa-miR-6880-5p, and miR-6802-5p is hs a-miR-6802-5p is the same as miR-6861-5p, and hsa-miR-686 1-5p, and miR-92b-5p is hsa-miR-92b-5p, and miR -1238-5p is hsa-miR-1238-5p, and miR-6851-5p is hsa-miR-6851-5p and miR-7704 are hsa-miR-7704 Therefore, miR-149-3p is hsa-miR-149-3p, and miR-468 9 is hsa-miR-4689, and miR-4688 is hsa-miR-4688. Yes, miR-125a-3p is hsa-miR-125a-3p, and miR-23 b-3p is hsa-miR-23b-3p, and miR-614 is hsa-miR-6 It is 14, and miR-1913 is hsa-miR-1913, and miR-16-5p hsa-miR-16-5p and miR-6717-5p are hsa-miR-67 17-5p, miR-3648 is hsa-miR-3648, miR-31 62-5p is hsa-miR-3162-5p, and miR-1909-3p is hsa -miR-1909-3p, and miR-8073 is hsa-miR-8073. miR-6769b-5p is hsa-miR-6769b-5p, and miR-68 36-3p is hsa-miR-6836-3p, and miR-4484 is hsa-mi It is R-4484, and miR-6819-5p is hsa-miR-6819-5p. , and miR-6794-5p is hsa-miR-6794-5p, (11) The device described. (13) The nucleic acid is a polynucleotide as shown in (a) to (e) below: (a) Represented by one of the sequence numbers 1-115, 117-189, and 666-675 Polynucleotides consisting of a base sequence or a base sequence in which u is t D, its variants, its derivatives, or fragments thereof containing 15 or more consecutive bases, (b) Represented by any of the sequence numbers 1-115, 117-189, and 666-675 Polynucleotides containing a base sequence (c) Represented by any of the sequence numbers 1-115, 117-189, and 666-675 From a base sequence or a base sequence complementary to the base sequence in which u is t Polynucleotides, their variants, their derivatives, or those containing 15 or more consecutive bases Fragments of (d) Represented by one of the sequence numbers 1-115, 117-189, and 666-675 A base sequence that includes a base sequence that is complementary to a base sequence in which u is t. polynucleotides, and (e) With any of the polynucleotides from (a) to (d) above under stringent conditions A hybridized polynucleotide, A polynucleotide selected from the group consisting of (11) or (12), the deba A chair. (14) The device is a different esophageal cancer marker, miR-575 and miR-2 (11)~ Further comprising a 4-3p polynucleotide and nucleic acids that can specifically bind to it. (13) Any of the devices described above. (15) miR-575 is hsa-miR-575, and miR-24-3p is hs The device described in (14), which is a-miR-24-3p. (16) The nucleic acid is a polynucleotide as shown in (f) to (j) below: (f) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide consisting of a base sequence t, its variants, its derivatives, or 15 or more The fragment containing consecutive bases, (g) Polynucleotides containing the base sequences represented by SEQ ID NOs. 116 and 676, (h) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide consisting of a base sequence complementary to a base sequence t, its mutant, and its induction. A body, or a fragment thereof containing 15 or more consecutive bases, (i) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide containing a base sequence complementary to the base sequence t, and (j) With any of the polynucleotides in (f) to (i) above under stringent conditions A hybridized polynucleotide, A polynucleotide selected from the group consisting of (14) or (15), the deba A chair. (17) The device is a different esophageal cancer marker, miR-675-5p, miR -486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, miR-6889-5p, miR- 6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, mi R-6090, miR-4730, miR-7106-5p, miR-3196, miR -5698, miR-6087, miR-4665-5p, miR-8059 and miR At least one polynucleotide selected from the group consisting of -6879-5p and specific The device according to any one of (11) to (16), further comprising nucleic acids that can bind to the device. (18) miR-675-5p is hsa-miR-675-5p, and miR-486 -3p is hsa-miR-486-3p, and miR-6777-5p is hsa-mi It is R-6777-5p, and miR-4497 is hsa-miR-4497, mi R-296-3p is hsa-miR-296-3p, and miR-6738-5p is h sa-miR-6738-5p is the same as miR-4731-5p, and hsa-miR-47 31-5p is, and miR-6889-5p is hsa-miR-6889-5p, miR-6786-5p is hsa-miR-6786-5p, and miR-92a-3 p is hsa-miR-92a-3p, and miR-4294 is hsa-miR-429 4, and miR-4763-3p is hsa-miR-4763-3p, and miR- 6076 is hsa-miR-6076, and miR-663a is hsa-miR-66 It is 3a, miR-760 is hsa-miR-760, and miR-4667-5p The one is hsa-miR-4667-5p, and the one with the miR-6090 is hsa-miR-609 0, miR-4730 is hsa-miR-4730, and miR-7106-5 p is hsa-miR-7106-5p, and miR-3196 is hsa-miR-31 96, miR-5698 is hsa-miR-5698, and miR-6087 is hsa-miR-6087 is hsa-miR-4665-5p is hsa-miR-4665 -5p, miR-8059 is hsa-miR-8059, and miR-6 The device described in (17), where 879-5p is hsa-miR-6879-5p. (19) The nucleic acid is a polynucleotide as shown in (k) to (o) below: (k) A base sequence represented by any of sequence numbers 190 to 214 or the said base sequence A polynucleotide consisting of a base sequence in which u is t, its variants, its derivatives, or 1 The fragment containing 5 or more consecutive bases, (l) Polynucleotides containing a base sequence represented by any of sequence numbers 190-214, (m) A base sequence represented by any of sequence numbers 190-214 or the said base sequence A polynucleotide consisting of a base sequence complementary to the base sequence where u is t, and its variants. Its derivative, or a fragment thereof containing 15 or more consecutive bases, (n) A base sequence represented by any of sequence numbers 190 to 214 or the said base sequence A polynucleotide containing a base sequence complementary to the base sequence in which u is t, and (o) With any of the polynucleotides (k) to (n) above under stringent conditions A hybridized polynucleotide, A polynucleotide selected from the group consisting of (17) or (18), the deba A chair. (20) The device is a device for measurement by hybridization technology. A device as described in any of (11) to (19). (21) The hybridization technology is nucleic acid array technology as described in (20) device. (22) The device is selected from all esophageal cancer markers described in (11) or (12) At least two polynucleotides selected and each of them can be specifically bound to at least A device according to any of (11) to (21), which also contains two nucleic acids. (23)A kit as described in any of (1) to (10) or any of (11) to (22) Using the device described above, the expression level of the target nucleic acid in the subject's sample is measured, and the measured The expression levels of the subjects, along with the control expression levels of healthy individuals, were used to determine which subjects had esophageal cancer. To evaluate in vitro whether or not one has esophageal cancer. Includes methods for detecting esophageal cancer. (24) The method according to (23), wherein the subject is a human. (25) The method according to (23) or (24), wherein the specimen is blood, serum, or plasma. <Definition of Terms> The terms used herein have the following definitions:

[0018] Abbreviations such as nucleotide, polynucleotide, DNA, and RNA are used to represent "base pairings." Guidelines for the Preparation of Specifications Including Columns or Amino Acid Sequences (edited by the Japan Patent Office) and The practice shall follow the conventions of the relevant technical field.

[0019] In this specification, "polynucleotide" means RNA, DNA, and RNA / DNA ( It is used for nucleic acids that include all of the following (chimeric): This includes both genomic DNA and synthetic DNA. Furthermore, the above RNA includes tota l RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA This includes both non-coding RNA and synthetic RNA. In this specification, "synthetic DNA" and "synthetic RNA" refer to a predetermined base sequence (natural type sequence). Either a non-natural sequence or a human-derived sequence may be used.) This refers to artificially produced DNA and RNA. In this specification, "non-natural sequence" is used in a broad sense. It is intended to be used in the sense of a sequence that differs from the natural type, for example, one or more nucleotides. A sequence containing substitutions, deletions, insertions and / or additions (i.e., a variant sequence), or one or more modifications. This includes sequences containing nucleotides (i.e., modified sequences), etc. Furthermore, in this specification... Polynucleotides are used interchangeably with nucleic acids.

[0020] In this specification, "fragment" refers to a part of a polynucleotide having a continuous base sequence. It is a polynucleotide with 15 or more bases, preferably 17 or more bases, more preferably 1 It is desirable that the string has a length of 9 bases or more.

[0021] In this specification, "gene" means not only RNA and double-stranded DNA, but also the constituent elements thereof. Each single strand of DNA, such as the positive strand (or sense strand) or the complementary strand (or antisense strand), is enclosed within it. It is used with the intention of including, and is not particularly limited by its length. Therefore, in this specification, "gene" refers to human genomic DNA unless otherwise specified. Contains double-stranded DNA, single-stranded DNA (positive strand), and single-stranded DNA having a sequence complementary to the positive strand. A (complementary strand, including cDNA), microRNA (miRNA), and fragments thereof, and any of their transcripts. Furthermore, the “gene” is a specific base sequence (or sequence). Not only the "genes" represented by numbers, but also the RNA and biology encoded by them. RNAs with equivalent functional properties, such as homologs or orthologues, It includes "nucleic acids" that encode variants such as genetic polymorphisms and derivatives. Nucleic acids that encode variants or derivatives include, specifically, the strings described later. Under normal conditions, the base sequence represented by any of sequence numbers 1 to 700, or the salt thereof A base sequence in which u is t, and a base sequence that hybridizes with the complementary sequence of that base sequence. One could list "nucleic acids." Note that "genes" do not refer to specific functional regions. For example, it may include expression regulatory regions, coding regions, exons, or introns. Furthermore, "genes" may be contained within cells, or they may be released outside the cell and exist independently. Often, they may also be contained within vesicles called exosomes.

[0022] In this specification, "exosome" refers to a vesicle enclosed in a lipid bilayer secreted by a cell. Exosomes originate from multivesicular endosomes and release RNA into the extracellular environment. They may contain "genes" such as DNA, or biomolecules such as proteins. Blood is known to be found in bodily fluids such as blood, serum, plasma, and lymph. Sosomes are sometimes commonly referred to as "exosomes."

[0023] In this specification, "transcript" refers to an RN synthesized using a gene's DNA sequence as a template. This refers to A. RNA polymerase is located in a region called the promoter, which is upstream of a gene. It binds to the DNA and attaches ribonucleotides to its 3' end so that it is complementary to the DNA base sequence. RNA is synthesized in this manner. This RNA contains not only the genes themselves, but also genes that regulate gene expression. From the transcription start site, including regions, coding regions, exons, or introns, the polyA sequence It includes the entire sequence, right down to the end.

[0024] Furthermore, in this specification, unless otherwise specified, "microRNA (miRNA)" means It is transcribed as a hairpin-like RNA precursor and possesses RNase III cleavage activity. It is cleaved by an sRNA-cleaving enzyme and incorporated into a protein complex called RISC, m It is intended to be used for non-coding RNAs of 15-25 nucleotides that are involved in the repression of RNA translation. ru.

[0025] Furthermore, the term "miRNA" as used herein is represented by a specific base sequence (or sequence number). Not only "miRNA," but also its precursor (pre-miRNA, pr i-miRNAs), and miRNAs that have equivalent biological function, such as congeners (i.e., This also includes variants such as homologs or orthologs, genetic polymorphisms, and derivatives. Such precursors, homologs, mutants, or derivatives include, specifically, miRBase r Identified using elease 20 (http: / / www.mirbase.org / ) It is possible to do so under the stringent conditions described later, any of sequence numbers 1 to 700 It has a base sequence that hybridizes with the complementary sequence of any of the specific base sequences represented by ka. "miRNA" can be cited. Furthermore, the "miRNA" used in this specification This may be the gene product of the miR gene, and such a gene product is a mature miRN. A (For example, 15-25 bases or 19-2 bases involved in the translational repression of mRNA as described above) 5-base non-coding RNA) or miRNA precursor (e.g., pre as described above) (Includes -miRNA or pri-miRNA)

[0026] In this specification, "probe" means RNA produced by gene expression or related thereto Polynucleotides and / or Polynucleotides used to specifically detect the resulting polynucleotides It includes complementary polynucleotides.

[0027] In this specification, "primer" means RNA or similar material produced by gene expression. Polynucleotides that specifically recognize and amplify the derived polynucleotides and / or It includes complementary polynucleotides. Here, complementary polynucleotides (complementary chain, reverse) A chain is a base sequence defined by any of sequence numbers 1 to 700, or the salt thereof. The full-length sequence of a polynucleotide consisting of a base sequence in which u is t, or the For a subarray (which we will call the positive chain for convenience), we have A:T(U) and G:C. Based on the base pairing relationship, it refers to polynucleotides that are basically complementary. However, such a complementary strand is only valid if it forms a sequence that is perfectly complementary to the base sequence of the target positive strand. Rather, a complementary relationship to the extent that it can hybridize with the target positive chain under stringent conditions. It may also have the following characteristics.

[0028] In this specification, “stringent conditions” means that the nucleic acid probe is in relation to other sequences. A greater degree than (for example, the average of background measurements + the standard of background measurements) This refers to the condition under which hybridization occurs with the target sequence using a measurement value of 2 times or more (approximate error). The triggering conditions are sequence-dependent and depend on the environment in which hybridization takes place. That's different. Control the stringency of hybridization and / or washing conditions. By doing so, target sequences that are 100% complementary to nucleic acid probes can be identified. Specific examples of "stringent conditions" will be discussed later.

[0029] In this specification, "Tm value" refers to the state in which the double-stranded portion of a polynucleotide is denatured into a single-stranded portion. This refers to the temperature at which double-stranded and single-stranded fibers exist in a 1:1 ratio.

[0030] In this specification, "mutant" refers to a naturally occurring variant of nucleic acid resulting from polymorphism, mutation, etc. A variant of, or any of the base sequences of SEQ ID NOs: 1-700, or a base sequence containing In a base sequence where u is t, or in a subsequence thereof, the deletion of one or more bases, Mutants including substitution, addition, or insertion, or each or a portion thereof of the said base sequence and about 90 Variations showing identity of % or more, approximately 95% or more, approximately 97% or more, approximately 98% or more, and approximately 99% or more. A body, or a polynucleotide or oligonucleotide containing the said base sequence or a partial sequence thereof. This refers to nucleic acids that hybridize with cytoplasm under the stringent conditions defined above.

[0031] In this specification, “multiple” means approximately 10, 9, 8, 7, 6, 5, 4, 3, or 2 units It means number.

[0032] In this specification, mutants are defined as mutants obtained using site-directed mutagenesis and PCR. It can be manufactured using well-known techniques such as the heterogeneous introduction method.

[0033] In this specification, "identity" refers to the protein or Using a gene search system, with or without introducing gaps, It can be determined (Zheng Zhang et al., 2000, J.Comput.Bi) ol., Vol. 7, pp. 203-214; Altschul, SF et al., 1990, Jou Journal of Molecular Biology, Vol. 215, pp. 403-410 Pearson, WR et al., 1988, Proc. Natl. Acad. Sci. USA, Vol. 85, pp. 2444-2448).

[0034] In this specification, "derivative" means modified nucleic acids, and not limited to, for example, fluorophores. Bellated derivatives, modified nucleotides (e.g., halogens, alkyls such as methyl, methoxy) Reconstitution of nucleotides and bases containing alkoxy, thio, carboxymethyl, and other groups. nucleotides that have undergone processes such as double bond saturation, deamination, and substitution of oxygen molecules with sulfur molecules. Derivatives including (etc.), PNA (peptide adhesive acid; Niels en, PE et al., 1991, Science, Vol. 254, pp. 1497-500), L NA (locked nucleic acid; Obika, S. et al., 1998, T This includes etrahedron Lett., Vol. 39, pp. 5401-5404, etc. It means.

[0035] In this specification, polynucleotides selected from the above-mentioned esophageal cancer markers, miRNAs "Nucleic acids" that can specifically bind to ocide are synthesized or prepared nucleic acids, specifically " A nucleic acid probe or primer is included to detect the presence or absence of esophageal cancer in the subject. For that purpose, or whether or not there is esophageal cancer, the extent of the disease, whether or not there is improvement in the esophageal cancer and the extent of improvement, To diagnose the sensitivity to treatment for esophageal cancer, or for the prevention, improvement, or treatment of esophageal cancer. These are used directly or indirectly to screen for candidate substances useful in treatment. In relation to the development of esophageal cancer, the sequence number is found in the body, particularly in specimens of bodily fluids such as blood and urine. It specifically recognizes and synthesizes a transcript represented by one of the numbers 1 to 700 or its cDNA synthetic nucleic acid. Includes nucleotides, oligonucleotides, and polynucleotides that can be combined. These nucleotides, oligonucleotides, and polynucleotides are based on the above properties. As a probe for detecting the above genes expressed in living organisms, tissues, or cells, It can be effectively used as a primer for amplifying the gene expressed in vivo. It is possible.

[0036] As used herein, the term "detection" can be replaced with the terms inspection, measurement, detection, or determination support. Also, in this specification, the term "evaluation" is used in the sense of including supporting diagnosis or evaluation based on inspection results or measurement results.

[0037] As used herein, the "subject" means mammals such as humans, primates including chimpanzees, pet animals such as dogs and cats, livestock animals such as cows, horses, sheep, and goats, and rodents such as mice and rats. Also, a "healthy subject" also means such a mammal that does not suffer from the cancer to be detected, that is, esophageal cancer.

[0038] As used herein, "P" or "P-value" indicates the probability that a statistic more extreme than the statistic actually calculated from the data is observed under the null hypothesis in a statistical test. Thus, the smaller the "P" or "P-value", the more a significant difference can be considered to exist between the comparison targets.

[0039] In this specification, "sensitivity" means the value of (number of true positives) / (number of true positives + number of false negatives). If the sensitivity is high, it becomes possible to detect esophageal cancer at an early stage, leading to the resection of the entire cancerous part and a reduction in the recurrence rate.

[0040] In this specification, "specificity" means (number of true negatives) / (number of true negatives + number of false positives). If the specificity is high, it prevents the unnecessary additional examinations due to misclassifying healthy subjects as esophageal cancer patients, leading to a reduction in the burden on patients and a reduction in medical costs.

[0041] In this specification, "accuracy" means the value of (number of true positives + number of true negatives) / (total number of cases). The accuracy indicates the percentage of all samples for which the classification result was correct, and the detection performance is evaluated. This will be the primary indicator.

[0042] In this specification, the term "specimen" that is subject to determination, detection, or diagnosis refers to the development of esophageal cancer, food As esophageal cancer progresses and the therapeutic effect against esophageal cancer is exerted, the expression of the gene of the present invention changes. This refers to tissues and biomaterials that undergo transformation. Specifically, this includes esophageal tissue and its surrounding blood vessels, lymph nodes, and Organs, organs suspected of metastasis, skin, and bodily fluids such as blood, urine, saliva, sweat, and tissue exudate. This refers to serum, plasma, and other substances prepared from blood, such as feces and hair. Furthermore, extraction from these substances... This refers to biological samples, specifically genes such as RNA and miRNA.

[0043] The term "hsa-miR-204-3p gene" or "hsa-miR" as used herein refers to the "hsa-miR" gene used in this specification. The term "-204-3p" refers to the hsa-miR sequence represented by Sequence ID No. 1. -204-3p gene (miRBase Accession No. MIMAT002) This includes 2693) and other species homologs or orthologs. hsa-miR -204-3p gene, Lim LP et al., 2003, Science, Vol. 299, p It can be obtained by the method described in 1540. Also, "hsa-miR-204 -3p is a precursor of "hsa-mir-204" (mi), which has a hairpin-like structure. RBase Accession No. MI0000284 (Sequence ID 215) is known. It is being done.

[0044] The term "hsa-miR-1247-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-1247-3p" refers to the hsa-m nucleotide sequence represented by Sequence ID No. 2. iR-1247-3p gene (miRBase Accession No. MIMAT) This includes 0022721) and other species homologs or orthologs. The miR-1247-3p gene was identified by Morin RD et al. in 2008 in Genome R. It can be obtained by the method described in es, Vol. 18, pp. 610-621. "hsa-miR-1247-3p" is a precursor of "hsa" which has a hairpin-like structure. -mir-1247” (miRBase Accession No. MI000638 2. Sequence ID 216 is known.

[0045] The term "hsa-miR-6875-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6875-5p" refers to the hsa-m nucleotide sequence represented by Sequence ID No. 3. iR-6875-5p gene (miRBase Accession No. MIMAT) This includes 0027650) and other species homologs or orthologs. The miR-6875-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6875-5p" has a hairpin-like structure as its precursor. hsa-mir-6875” (miRBase Accession No. MI002 (2722, Sequence ID 217) is known.

[0046] The term "hsa-miR-6857-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6857-5p" refers to hsa-m consisting of the nucleotide sequence represented by SEQ ID NO: 4 iR-6857-5p gene (miRBase Accession No. MIMAT 0027614) and other homologs or orthologs of other species, etc. are included. hsa- The miR-6857-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6857-5p" has a hairpin-like structure as its precursor, " hsa-mir-6857" (miRBase Accession No. MI002 2703, SEQ ID NO: 218) is known.

[0047] As used herein, the term "hsa-miR-6726-5p gene" or "hsa-mi R-6726-5p" refers to hsa-m consisting of the nucleotide sequence represented by SEQ ID NO: 5 iR-6726-5p gene (miRBase Accession No. MIMAT 0027353) and other homologs or orthologs of other species, etc. are included. hsa- The miR-‑6726-5p gene can be obtained by the method described in Ladewig E et al., 2012, Genome Res, 22, p1634-1645. Also, "hsa-miR-6726-5p" has a hairpin-like structure as its precursor, " hsa-mir-6726" (miRBase Accession No. MI002 2571, SEQ ID NO: 219) is known.

[0048] As used herein, the term "hsa-miR-3188 gene" or "hsa-miR-3 The term "188" refers to the nucleotide sequence hsa-miR-318, represented by Sequence ID No. 6. 8 genes (miRBase Accession No. MIMAT0015070) and This includes homologs or orthologs of other biological species. hsa-miR-3188 The gene is described in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. It can be obtained by the method described. Also, "hsa-miR-3188" is the previous The "hsa-mir-3188" (miRBase Ac) has a hairpin-like structure as its chassis. Cession No. MI0014232 (Sequence No. 220) is known.

[0049] The term "hsa-miR-8069 gene" or "hsa-miR-8" as used herein refers to the "hsa-miR-8" gene used in this specification. The term "069" refers to the nucleotide sequence hsa-miR-806, represented by Sequence ID No. 7. 9 genes (miRBase Accession No. MIMAT0030996) and This includes homologs or orthologs of other biological species. hsa-miR-8069 The gene is described in Wang HJ et al., 2013, Shock, Vol. 39, pp. 480-487. It can be obtained by the method described. Also, "hsa-miR-8069" is the previous The "hsa-mir-8069" (miRBase Ac) has a hairpin-like structure as its chassis. Cession No. MI0025905 (Sequence No. 221) is known.

[0050] The term "hsa-miR-4257 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4257 gene" used herein. The term "257" refers to the nucleotide sequence hsa-miR-425, represented by Sequence ID No. 8. 7 genes (miRBase Accession No. MIMAT0016878) and This includes homologs or orthologs of other biological species. hsa-miR-4257 The gene is described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. It can be obtained by the method described above. Also, "hsa-miR-4257" is its precursor. The "hsa-mir-4257" (miRBase Acc) has a hairpin-like structure as its body. The known sequence number is MI0015856 (Sequence No. 222).

[0051] The term "hsa-miR-1343-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-1343-3p" refers to the hsa-m nucleotide sequence represented by Sequence ID No. 9. iR-1343-3p gene (miRBase Accession No. MIMAT) This includes 0019776) and other species homologs or orthologs. The miR-1343-3p gene was identified by Persson H et al. in 2011 as a cancer cell. It can be obtained by the method described in Res, Vol. 71, pp. 78-86. Also, "h "sa-miR-1343-3p" has a hairpin-like structure as its precursor, "hsa- mir-1343” (miRBase Accession No. MI0017320 (Sequence ID 223) is known.

[0052] The term "hsa-miR-7108-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-7108-5p" refers to the nucleotide sequence represented by Sequence ID No. 10, which is hsa- miR-7108-5p gene (miRBase Accession No. MIMA) This includes T0028113) and other species homologs or orthologs. The -miR-7108-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-7108-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-7108" (miRBase Accession No. MI00 (22959, Sequence ID 224) is known.

[0053] The term "hsa-miR-6825-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6825-5p" refers to the nucleotide sequence represented by Sequence ID No. 11, which is hsa- miR-6825-5p gene (miRBase Accession No. MIMA) This includes T0027550) and other species homologs or orthologs. The -miR-6825-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6825-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6825" (miRBase Accession No. MI00 (22670, Sequence ID 225) is known.

[0054] The term "hsa-miR-7641 gene" or "hsa-miR-7" as used herein refers to the "hsa-miR-7641 gene" used in this specification. The term "641" refers to the nucleotide sequence hsa-miR-76 represented by Sequence ID No. 12. 41 genes (miRBase Accession No. MIMAT0029782) This includes homologs or orthologs of other biological species. hsa-miR-7641 The gene was identified by Yoo JK et al., 2013, Arch Pharm Res, Vol. 36, p. 3. It can be obtained by the method described in 53-358. Also, "hsa-miR-7 "641" has a hairpin-like structure as its precursor, "hsa-mir-7641-1, hsa-mir-7641-2” (miRBase Accession No.MI0 The following are known: 024975, MI0024976, and sequence numbers 226 and 227.

[0055] The term "hsa-miR-3185 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3185 gene" or "hsa-miR-3 The term "185" refers to the nucleotide sequence hsa-miR-31 represented by Sequence ID No. 13. 85 genes (miRBase Accession No. MIMAT0015065) This includes homologs or orthologs of other biological species. hsa-miR-3185 The gene was described in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. It can be obtained by the method described. Also, "hsa-miR-3185" is "hsa-mir-3185" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0014227 (Sequence No. 228) is known.

[0056] The "hsa-miR-4746-3p gene" or "hsa-mi" used in this specification The term "R-4746-3p" refers to the hsa- sequence represented by Sequence ID No. 14. miR-4746-3p gene (miRBase Accession No. MIMA) This includes T0019881) and other species homologs or orthologs. -miR-4746-3p gene, Persson H et al., 2011, Cancer It can be obtained by the method described in Res, Vol. 71, pp. 78-86. "hsa-miR-4746-3p" is a precursor of "hsa" which has a hairpin-like structure. -mir-4746” (miRBase Accession No. MI001738 5. Sequence ID 229 is known.

[0057] The term "hsa-miR-6791-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6791-5p" refers to the nucleotide sequence represented by Sequence ID No. 15, which is hsa- miR-6791-5p gene (miRBase Accession No. MIMA) This includes T0027482) and other species homologs or orthologs. The -miR-6791-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6791-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6791" (miRBase Accession No. MI00 Number 22636 (sequence number 230) is known.

[0058] The term "hsa-miR-6893-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6893-5p" refers to the nucleotide sequence represented by Sequence ID No. 16, which is hsa- miR-6893-5p gene (miRBase Accession No. MIMA) This includes T0027686 and other homologs or orthologs of biological species. The -miR-6893-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6893-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6893" (miRBase Accession No. MI00 (22740, Sequence ID 231) is known.

[0059] The terms "hsa-miR-4433b-3p gene" or "hsa-m" used in this specification refer to the "hsa-miR-4433b-3p gene" or "hsa-m" gene. The term "iR-4433b-3p" refers to the nucleotide sequence represented by Sequence ID No. 17. a-miR-4433b-3p gene (miRBase Accession No. M This includes IMAT0030414) and other species homologs or orthologs. The hsa-miR-4433b-3p gene was identified by Ple H et al., 2012, PLoS O It can be obtained by the method described in ne, volume 7, e50746. Also, "hsa -miR-4433b-3p has a hairpin-like structure as its precursor, hsa-m ir-4433b” (miRBase Accession No. MI0025511 (Sequence ID 232) is known.

[0060] The term "hsa-miR-3135b gene" or "hsa-miR-" used herein refers to the "hsa-miR-3135b gene" used herein. The term "3135b" refers to the hsa-miR- nucleotide sequence represented by Sequence ID No. 18. 3135b gene (miRBase Accession No. MIMAT00189) 85) and other species homologs or orthologs are included. hsa-miR-3 The 135b gene was identified by Jima DD et al., 2010, Blood, Vol. 116, e118- It can be obtained by the method described in e127. Also, "hsa-miR-313 "5b" is a precursor of "hsa-mir-3135b" (m iRBase Accession No. MI0016809 (Sequence ID 233) is known It is being done.

[0061] The term "hsa-miR-6781-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6781-5p" refers to the nucleotide sequence represented by Sequence ID No. 19, which is hsa- miR-6781-5p gene (miRBase Accession No. MIMA) This includes T0027462) and other species homologs or orthologs. The -miR-6781-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6781-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6781" (miRBase Accession No. MI00 Sequence ID 22626 (Sequence ID 234) is known.

[0062] The term "hsa-miR-1908-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-1908-5p" refers to the nucleotide sequence represented by Sequence ID No. 20, which is hsa- miR-1908-5p gene (miRBase Accession No. MIMA) This includes T0007881) and other species homologs or orthologs, etc. The -miR-1908-5p gene was identified by Bar M et al., 2008, in Stem Cells. It can be obtained by the method described in Volume 26, pp. 2496-2505. "hsa-miR-1908-5p" is a precursor that takes on a hairpin-like structure. -mir-1908” (miRBase Accession No. MI000832 9. Sequence ID 235 is known.

[0063] The terms "hsa-miR-4792 gene" or "hsa-miR-4" used herein refer to the "hsa-miR-4" gene. The term "792" refers to the nucleotide sequence hsa-miR-47 represented by Sequence ID No. 21. 92 genes (miRBase Accession No. MIMAT0019964) This includes homologs or orthologs of other biological species. hsa-miR-4792 Genes are described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 78. It can be obtained by the method described in -86. Also, "hsa-miR-4792 " is a precursor that takes the hairpin-like structure of "hsa-mir-4792" (miRB The known sequence number (Accession No. MI0017439, Sequence ID 236) is Yes, they are.

[0064] The term "hsa-miR-7845-5p gene" or "hsa-mi" as used herein refers to the same gene used in this specification. The term "R-7845-5p" refers to the nucleotide sequence represented by Sequence ID No. 22, which is hsa- miR-7845-5p gene (miRBase Accession No. MIMA) This includes T0030420) and other species homologs or orthologs. The -miR-7845-5p gene was identified by Ple H et al., 2012, PLoS One, 7 It can be obtained by the method described in volume e50746. Also, "hsa-miR "-7845-5p" has a hairpin-like structure as its precursor, "hsa-mir-78 45" (miRBase Accession No. MI0025515, Sequence ID 2) 37) is known.

[0065] The term "hsa-miR-4417 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "417" refers to the nucleotide sequence hsa-miR-44, represented by Sequence ID No. 23. 17 genes (miRBase Accession No. MIMAT0018929) This includes homologs or orthologs of other biological species. hsa-miR-4417 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4417" is Its precursor is "hsa-mir-4417" (miRBase), which has a hairpin-like structure. Accession No. MI0016753 (Sequence ID 238) is known.

[0066] The term "hsa-miR-3184-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-3184-5p" refers to the nucleotide sequence represented by Sequence ID No. 24, which is hsa- miR-3184-5p gene (miRBase Accession No. MIMA) This includes T0015064) and other species homologs or orthologs. The -miR-3184-5p gene was identified by Stark MS et al., 2010, PLoS On It can be obtained by the method described in e, Volume 5, e9685. Also, "hsa-m "iR-3184-5p" has a hairpin-like structure as its precursor, "hsa-mir- 3184 (miRBase Accession No. MI0014226, Sequence Number) Number 239 is known.

[0067] The term "hsa-miR-1225-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-1225-5p" refers to the nucleotide sequence represented by Sequence ID No. 25, which is hsa- miR-1225-5p gene (miRBase Accession No. MIMA) This includes T0005572) and other species homologs or orthologs. The -miR-1225-5p gene was identified by Berezikov E et al., 2007, Mol It can also be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1225-5p" has a hairpin-like structure as its precursor "h sa-mir-1225” (miRBase Accession No. MI0006 311 (Sequence ID 240) is known.

[0068] The term "hsa-miR-1231 gene" or "hsa-miR-1" as used herein refers to the "hsa-miR-1231 gene" used herein. The term "231" refers to the nucleotide sequence hsa-miR-12 represented by sequence number 26. 31 genes (miRBase Accession No. MIMAT0005586) This includes homologs or orthologs of other biological species. hsa-miR-1231 The gene is from Berezikov E et al., 2007, Mol Cell, Vol. 28, p. 32. It can be obtained by the method described in 8-336. Also, "hsa-miR-12 "31" is a precursor that takes the hairpin-like structure of "hsa-mir-1231" (mi RBase Accession No. MI0006321 (Sequence ID 241) is known. It is being done.

[0069] The term "hsa-miR-1225-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-1225-3p" refers to the nucleotide sequence represented by Sequence ID No. 27, which is hsa- miR-1225-3p gene (miRBase Accession No. MIMA) This includes T0005573) and other species homologs or orthologs, etc. The -miR-1225-3p gene was identified by Berezikov E et al., 2007, Mol It can also be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1225-3p" has a hairpin-like structure as its precursor. sa-mir-1225” (miRBase Accession No. MI0006 311 (Sequence ID 240) is known.

[0070] The term "hsa-miR-150-3p gene" or "hsa-miR" used in this specification refers to the "hsa-miR" gene. The term "-150-3p" refers to the nucleotide sequence represented by Sequence ID No. 28, which is hsa-mi R-150-3p gene (miRBase Accession No. MIMAT00) This includes 04610) and other species homologs or orthologs. The R-150-3p gene was identified by Lagos-Quintana M et al. in 2002, Cur It can be obtained by the method described in Biol, Vol. 12, pp. 735-739. Furthermore, "hsa-miR-150-3p" has a hairpin-like structure as its precursor. hsa-mir-150” (miRBase Accession No. MI0000 479 (Sequence ID 242) is known.

[0071] The term "hsa-miR-4433-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-4433-3p" refers to the nucleotide sequence represented by Sequence ID No. 29, which is hsa- miR-4433-3p gene (miRBase Accession No. MIMA) This includes T0018949 and other homologs or orthologs of biological species. -miR-4433-3p gene, Jima DD et al., 2010, Blood, 11 It can be obtained by the method described in Volume 6, e118-e127. Also, "hsa -miR-4433-3p is a precursor of hsa-mi, which has a hairpin-like structure. r-4433” (miRBase Accession No. MI0016773, Column number 243) is known.

[0072] The term "hsa-miR-6125 gene" or "hsa-miR-6" as used herein refers to the "hsa-miR-6" gene used in this specification. The term "125" refers to the nucleotide sequence hsa-miR-61 represented by sequence number 30. 25 genes (miRBase Accession No. MIMAT0024598) This includes homologs or orthologs of other biological species. hsa-miR-6125 The gene was identified by Smith JL et al., 2012, J Virol, Vol. 86, pp. 5278-5. It can be obtained by the method described in 287. Also, "hsa-miR-6125 " is a precursor that takes the hairpin-like structure of "hsa-mir-6125" (miRB The known sequence number (Accession No. MI0021259, Sequence ID 244) is Yes, they are.

[0073] The term "hsa-miR-4513 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "513" refers to the nucleotide sequence hsa-miR-45 represented by Sequence ID No. 31. 13 genes (miRBase Accession No. MIMAT0019050) This includes homologs or orthologs of other biological species. hsa-miR-4513 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4513" is Its precursor is "hsa-mir-4513" (miRBase), which has a hairpin-like structure. Accession No. MI0016879 (Sequence ID 245) is known.

[0074] The term "hsa-miR-6787-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6787-5p" refers to the nucleotide sequence represented by Sequence ID No. 32, which is hsa- miR-6787-5p gene (miRBase Accession No. MIMA) This includes T0027474) and other species homologs or orthologs. The -miR-6787-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6787-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6787" (miRBase Accession No. MI00 (22632, Sequence ID 246) is known.

[0075] The term "hsa-miR-6784-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6784-5p" refers to the nucleotide sequence represented by Sequence ID No. 33, which is hsa- miR-6784-5p gene (miRBase Accession No. MIMA) This includes T0027468 and other homologs or orthologs of biological species. The -miR-6784-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6784-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6784" (miRBase Accession No. MI00 (22629, Sequence ID 247) is known.

[0076] The term "hsa-miR-615-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-615-5p" refers to the hsa-mi base sequence represented by sequence number 34. R-615-5p gene (miRBase Accession No. MIMAT00) This includes 04804) and other species homologs or orthologs. The R-615-5p gene was identified by Cummins JM et al., 2006, in Proc Natl. The method described in Acad Sci USA, Vol. 103, pp. 3687-3692 Therefore, it can be obtained. Also, "hsa-miR-615-5p" is used as its precursor. The "hsa-mir-615" (miRBase Accessi) has a hairpin-like structure. (No.MI0003628, Sequence ID 248) is known.

[0077] The "hsa-miR-6765-3p gene" or "hsa-mi" used in this specification The term "R-6765-3p" refers to the nucleotide sequence represented by Sequence ID No. 35, which is hsa- miR-6765-3p gene (miRBase Accession No. MIMA) This includes T0027431) and other species homologs or orthologs. The -miR-6765-3p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6765-3p" adopts a hairpin-like structure as its precursor. "hsa-mir-6765" (miRBase Accession No. MI00 (22610, Sequence ID 249) is known.

[0078] The term "hsa-miR-5572 gene" or "hsa-miR-5" as used herein refers to the "hsa-miR-5" gene used in this specification. The term "572" refers to the nucleotide sequence hsa-miR-55 represented by sequence number 36. 72 genes (miRBase Accession No. MIMAT0022260) This includes homologs or orthologs of other biological species. hsa-miR-5572 The gene was identified by Tandon M et al., 2012, Oral Dis, Vol. 18, pp. 127-1. It can be obtained by the method described in 31. Also, "hsa-miR-5572" Its precursor is "hsa-mir-5572" (miRBa Accession No. MI0019117, Sequence ID 250) is known. ru.

[0079] The term "hsa-miR-6842-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6842-5p" refers to the nucleotide sequence represented by Sequence ID No. 37, which is hsa- miR-6842-5p gene (miRBase Accession No. MIMA) This includes T0027586) and other species homologs or orthologs. The -miR-6842-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6842-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-6842" (miRBase Accession No. MI00 Number 22688 (sequence number 251) is known.

[0080] The term "hsa-miR-8063 gene" or "hsa-miR-8" as used herein refers to the "hsa-miR-8" gene used in this specification. The term "063" refers to the hsa-miR-80 sequence represented by Sequence ID No. 38. 63 genes (miRBase Accession No. MIMAT0030990) This includes homologs or orthologs of other biological species. hsa-miR-8063 The gene is described in Wang HJ et al., 2013, Shock, Vol. 39, pp. 480-487. It can be obtained by the method described. Also, "hsa-miR-8063" is "hsa-mir-8063" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0025899 (Sequence No. 252) is known.

[0081] The terms "hsa-miR-6780b-5p gene" or "hsa-m" used herein refer to the "hsa-miR-6780b-5p gene" used herein. The term "iR-6780b-5p" refers to the nucleotide sequence represented by Sequence ID No. 39. a-miR-6780b-5p gene (miRBase Accession No. M This includes IMAT0027572) and other species homologs or orthologs. The hsa-miR-6780b-5p gene was identified by Ladewig E et al. in 2012. It can be obtained by the method described in Nome Res, Vol. 22, pp. 1634-1645. Yes, it is possible. Also, "hsa-miR-6780b-5p" is a hairpin-like precursor. The structure is "hsa-mir-6780b" (miRBase Accession N o.MI0022681 (Sequence ID 253) is known.

[0082] The term "hsa-miR-187-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-187-5p" refers to the hsa-mi base sequence represented by Sequence ID No. 40. R-187-5p gene (miRBase Accession No. MIMAT00) This includes 04561) and other species homologs or orthologs. The R-187-5p gene was identified by Lim LP et al., 2003, Science, Vol. 299. It can be obtained by the method described on page 1540. Also, "hsa-miR-18 "7-5p" is a precursor of "hsa-mir-187" (m), which has a hairpin-like structure. iRBase Accession No. MI0000274 (Sequence ID 254) is known It is being done.

[0083] The terms "hsa-miR-128-1-5p gene" or "hsa-m" used herein refer to the "hsa-miR-128-1-5p gene" used herein. The term "iR-128-1-5p" refers to the nucleotide sequence represented by sequence number 41. a-miR-128-1-5p gene (miRBase Accession No. M This includes IMAT0026477) and other species homologs or orthologs. The hsa-miR-128-1-5p gene was identified by Lagos-Quintana M et al. Obtained by the method described in Curr Biol, Vol. 12, pp. 735-739, 2002. It is possible to do so. Also, "hsa-miR-128-1-5p" is a precursor of "hsa-mir-128-1" (miRBase Accessi) adopts an appin-like structure. The code (on No.MI0000447, Sequence ID 255) is known.

[0084] The term "hsa-miR-6729-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi The term "R-6729-5p" refers to the nucleotide sequence represented by Sequence ID No. 42, which is hsa- miR-6729-5p gene (miRBase Accession No. MIMA) This includes T0027359) and other species homologs or orthologs. The -miR-6729-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6729-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-6729" (miRBase Accession No. MI00 (22574, Sequence ID 256) is known.

[0085] The term "hsa-miR-6741-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6741-5p" refers to the nucleotide sequence represented by Sequence ID No. 43, which is hsa- miR-6741-5p gene (miRBase Accession No. MIMA) This includes T0027383) and other species homologs or orthologs. The -miR-6741-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6741-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-6741" (miRBase Accession No. MI00 Numbers 22586 and SEQ ID NO: 257 are known.

[0086] The "hsa-miR-6757-5p gene" or "hsa-mi" used herein The term "R-6757-5p" refers to the nucleotide sequence represented by Sequence ID No. 44, which is hsa- miR-6757-5p gene (miRBase Accession No. MIMA) This includes T0027414) and other species homologs or orthologs, etc. The -miR-6757-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6757-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6757" (miRBase Accession No. MI00 (22602, Sequence ID 258) is known.

[0087] The term "hsa-miR-7110-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-7110-5p" refers to the nucleotide sequence represented by Sequence ID No. 45, which is hsa- miR-7110-5p gene (miRBase Accession No. MIMA) This includes T0028117) and other species homologs or orthologs. The -miR-7110-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-7110-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-7110" (miRBase Accession No. MI00 (22961, Sequence ID 259) is known.

[0088] The term "hsa-miR-7975 gene" or "hsa-miR-7" as used herein refers to the "hsa-miR-7975 gene" used herein. The term "975" refers to the nucleotide sequence hsa-miR-79 represented by sequence number 46. 75 genes (miRBase Accession No. MIMAT0031178) This includes homologs or orthologs of other biological species. hsa-miR-7975 The gene was identified by Velthut-Meikas A et al., 2013, in Mol Endocri. It can be obtained by the method described in nol, online edition. Also, "hsa- "miR-7975" has a hairpin-like structure as its precursor, "hsa-mir-79 75" (miRBase Accession No. MI0025751, Sequence ID 2) 60) is known.

[0089] The term "hsa-miR-1233-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-1233-5p" refers to the nucleotide sequence represented by sequence number 47, which is hsa- miR-1233-5p gene (miRBase Accession No. MIMA) This includes T0022943 and other species homologs or orthologs. The -miR-1233-5p gene was identified by Berezikov E et al., 2007, Mol It can also be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1233-5p" has a hairpin-like structure as its precursor "h sa-mir-1233-1, hsa-mir-1233-2” (miRBase Ac Cession No. MI0006323, MI0015973, Sequence ID 261, 2 62) is known.

[0090] The term "hsa-miR-6845-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6845-5p" refers to the nucleotide sequence represented by Sequence ID No. 48, which is hsa- miR-6845-5p gene (miRBase Accession No. MIMA) This includes T0027590 and other species homologs or orthologs. The -miR-6845-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6845-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6845" (miRBase Accession No. MI00 (22691, Sequence ID 263) is known.

[0091] The term "hsa-miR-3937 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3937 gene" or "hsa-miR-3 The term "937" refers to the nucleotide sequence hsa-miR-39 represented by sequence number 49. 37 genes (miRBase Accession No. MIMAT0018352) This includes homologs or orthologs of other biological species. hsa-miR-3937 The gene was described in Liao JY et al., 2010, PLoS One, Vol. 5, e10563. It can be obtained by the method described. Also, "hsa-miR-3937" is "hsa-mir-3937" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0016593 (Sequence No. 264) is known.

[0092] The term "hsa-miR-4467 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "467" refers to the nucleotide sequence hsa-miR-44, represented by Sequence ID No. 50. 67 genes (miRBase Accession No. MIMAT0018994) This includes homologs or orthologs of other biological species. hsa-miR-4467 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4467" is Its precursor is "hsa-mir-4467" (miRBase), which has a hairpin-like structure. Accession No. MI0016818 (Sequence ID 265) is known.

[0093] The term "hsa-miR-7109-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-7109-5p" refers to the nucleotide sequence represented by Sequence ID No. 51, which is hsa- miR-7109-5p gene (miRBase Accession No. MIMA) This includes T0028115) and other species homologs or orthologs, etc. The -miR-7109-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-7109-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-7109" (miRBase Accession No. MI00 (22960, Sequence ID 266) is known.

[0094] The term "hsa-miR-6088 gene" or "hsa-miR-6" as used herein refers to the "hsa-miR-6" gene used in this specification. The term "088" refers to the hsa-miR-60 sequence represented by Sequence ID No. 52. 88 genes (miRBase Accession No. MIMAT0023713) This includes homologs or orthologs of other biological species. hsa-miR-6088 The gene was identified by Yoo JK et al., 2012, Stem Cells Dev, Vol. 21, p. 2. It can be obtained by the method described in 049-2057. Also, "hsa-miR "-6088" is a precursor that takes on a hairpin-like structure, "hsa-mir-6088". (miRBase Accession No. MI0020365, Sequence ID 267) It is known that...

[0095] The term "hsa-miR-6782-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6782-5p" refers to the nucleotide sequence represented by Sequence ID No. 53, which is hsa- miR-6782-5p gene (miRBase Accession No. MIMA) This includes T0027464 and other species homologs or orthologs. The -miR-6782-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6782-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6782" (miRBase Accession No. MI00 (22627, Sequence ID 268) is known.

[0096] The term "hsa-miR-5195-3p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-5195-3p" refers to the nucleotide sequence represented by sequence number 54, which is hsa- miR-5195-3p gene (miRBase Accession No. MIMA) This includes T0021127) and other species homologs or orthologs. The -miR-5195-3p gene was identified by Schotte D et al., 2011, and Leukem. It can be obtained by the method described in ia, volume 25, pp. 1389-1399. "hsa-miR-5195-3p" has a hairpin-like structure as its precursor "h sa-mir-5195” (miRBase Accession No. MI0018 Sequence ID 174 (Sequence ID 269) is known.

[0097] The term "hsa-miR-4454 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "454" refers to the nucleotide sequence hsa-miR-44, represented by Sequence ID No. 55. 54 genes (miRBase Accession No. MIMAT0018976) This includes homologs or orthologs of other biological species. hsa-miR-4454 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4454" is Its precursor is "hsa-mir-4454" (miRBase), which has a hairpin-like structure. Accession No. MI0016800 (Sequence ID 270) is known.

[0098] The term "hsa-miR-6724-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6724-5p" refers to the nucleotide sequence represented by sequence number 56, which is hsa- miR-6724-5p gene (miRBase Accession No. MIMA) This includes T0025856) and other species homologs or orthologs. -miR-6724-5p gene: Li Y et al., 2012, Gene, Vol. 497, p It can be obtained by the method described in 330-335. Also, "hsa-miR- "6724-5p" has a hairpin-like structure as its precursor, "hsa-mir-672 4" (miRBase Accession No. MI0022559, Sequence ID 27) 1) is known.

[0099] The terms "hsa-miR-8072 gene" or "hsa-miR-8" used herein refer to the "hsa-miR-8" gene used herein. The term "072" refers to the hsa-miR-80 sequence represented by Sequence ID No. 57. 72 genes (miRBase Accession No. MIMAT0030999) This includes homologs or orthologs of other biological species. hsa-miR-8072 The gene is described in Wang HJ et al., 2013, Shock, Vol. 39, pp. 480-487. It can be obtained by the method described. Also, "hsa-miR-8072" is "hsa-mir-8072" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0025908 (Sequence No. 272) is known.

[0100] The term "hsa-miR-4516 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "516" refers to the nucleotide sequence hsa-miR-45 represented by sequence number 58. 16 genes (miRBase Accession No. MIMAT0019053) This includes homologs or orthologs of other biological species. hsa-miR-4516 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4516" is Its precursor is "hsa-mir-4516" (miRBase), which has a hairpin-like structure. Accession No. MI0016882 (Sequence ID 273) is known.

[0101] The "hsa-miR-6756-5p gene" or "hsa-mi" used herein The term "R-6756-5p" refers to the nucleotide sequence represented by Sequence ID No. 59, which is hsa- miR-6756-5p gene (miRBase Accession No. MIMA) This includes T0027412) and other species homologs or orthologs. The -miR-6756-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6756-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6756" (miRBase Accession No. MI00 (22601, Sequence ID 274) is known.

[0102] The "hsa-miR-4665-3p gene" or "hsa-mi" used in this specification The term "R-4665-3p" refers to the nucleotide sequence represented by Sequence ID No. 60, which is hsa- miR-4665-3p gene (miRBase Accession No. MIMA) This includes T0019740) and other species homologs or orthologs. -miR-4665-3p gene, Persson H et al., 2011, Cancer It can be obtained by the method described in Res, Vol. 71, pp. 78-86. "hsa-miR-4665-3p" is a precursor of "hsa" which has a hairpin-like structure. -mir-4665” (miRBase Accession No. MI001729 5. Sequence ID 275 is known.

[0103] The term "hsa-miR-6826-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6826-5p" refers to the nucleotide sequence represented by Sequence ID No. 61, which is hsa- miR-6826-5p gene (miRBase Accession No. MIMA) This includes T0027552) and other species homologs or orthologs. The -miR-6826-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6826-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6826" (miRBase Accession No. MI00 (22671, Sequence ID 276) is known.

[0104] The term "hsa-miR-6820-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6820-5p" refers to the nucleotide sequence represented by Sequence ID No. 62, which is hsa- miR-6820-5p gene (miRBase Accession No. MIMA) This includes T0027540) and other species homologs or orthologs. The -miR-6820-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6820-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-6820" (miRBase Accession No. MI00 (22665, Sequence ID 277) is known.

[0105] The term "hsa-miR-6887-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6887-5p" refers to the nucleotide sequence represented by Sequence ID No. 63, which is hsa- miR-6887-5p gene (miRBase Accession No. MIMA) This includes T0027674 and other species homologs or orthologs. The -miR-6887-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6887-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6887" (miRBase Accession No. MI00 (22734, Sequence ID 278) is known.

[0106] The "hsa-miR-3679-5p gene" or "hsa-mi" used herein The term "R-3679-5p" refers to the nucleotide sequence represented by Sequence ID No. 64, which is hsa- miR-3679-5p gene (miRBase Accession No. MIMA) This includes T0018104) and other species homologs or orthologs. The -miR-3679-5p gene was identified by Creighton CJ et al., 2010, PLo It can be obtained by the method described in S One, Vol. 5, e9637. Also, "h "sa-miR-3679-5p" has a hairpin-like structure as its precursor, "hsa- mir-3679” (miRBase Accession No. MI0016080 (Sequence number 279) is known.

[0107] The term "hsa-miR-7847-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-7847-3p" refers to the nucleotide sequence represented by Sequence ID No. 65, which is hsa- miR-7847-3p gene (miRBase Accession No. MIMA) This includes T0030422) and other species homologs or orthologs. The -miR-7847-3p gene was identified by Ple H et al., 2012, PLoS One, 7 It can be obtained by the method described in volume e50746. Also, "hsa-miR "-7847-3p" has a hairpin-like structure as its precursor, "hsa-mir-78 47" (miRBase Accession No. MI0025517, Sequence ID 2) 80) is known.

[0108] The term "hsa-miR-6721-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6721-5p" refers to the nucleotide sequence represented by sequence number 66, which is hsa- miR-6721-5p gene (miRBase Accession No. MIMA) This includes T0025852) and other species homologs or orthologs. -miR-6721-5p gene: Li Y et al., 2012, Gene, Vol. 497, p It can be obtained by the method described in 330-335. Also, "hsa-miR- "6721-5p" has a hairpin-like structure as its precursor, "hsa-mir-672 1" (miRBase Accession No. MI0022556, Sequence ID 28) 1) is known.

[0109] The terms "hsa-miR-3622a-5p gene" or "hsa-m" used herein refer to the "hsa-miR-3622a-5p gene" used herein. The term "iR-3622a-5p" refers to the nucleotide sequence represented by Sequence ID No. 67. a-miR-3622a-5p gene (miRBase Accession No. M This includes IMAT0018003) and other species homologs or orthologs. The hsa-miR-3622a-5p gene was identified by Witten D et al. in 2010 at BMC. It can be obtained by the method described in Biol, Vol. 8, p. 58. Also, "hsa -miR-3622a-5p is a precursor of hsa-m, which has a hairpin-like structure. ir-3622a” (miRBase Accession No. MI0016013 (Sequence ID 282) is known.

[0110] The term "hsa-miR-939-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-939-5p" refers to the hsa-mi base sequence represented by sequence number 68. R-939-5p gene (miRBase Accession No. MIMAT00) This includes 04982) and other species homologs or orthologs. The R-939-5p gene was identified by Lui WO et al., 2007, Cancer Res, 67. It can be obtained by the method described in Volume 6031-6043. Also, "hsa "-miR-939-5p" has a hairpin-like structure as its precursor, "hsa-mir -939 (miRBase Accession No. MI0005761, Sequence Number) No. 283 is known.

[0111] The "hsa-miR-602 gene" or "hsa-miR-60" used in this specification The term "2" refers to the hsa-miR-602 gene, which consists of the base sequence represented by Sequence ID No. 69. The gene (miRBase Accession No. MIMAT0003270) and its It includes homologs or orthologs of other species. The hsa-miR-602 gene is , Cummins JM et al. 2006, Proc Natl Acad Sci U It can be obtained by the method described in SA, Vol. 103, pp. 3687-3692. Furthermore, "hsa-miR-602" has a hairpin-like structure as its precursor. -mir-602” (miRBase Accession No. MI0003615 (Sequence ID 284) is known.

[0112] The term "hsa-miR-7977 gene" or "hsa-miR-7" as used herein refers to the "hsa-miR-7977 gene" used in this specification. The term "977" refers to the nucleotide sequence hsa-miR-79, represented by sequence number 70. 77 genes (miRBase Accession No. MIMAT0031180) This includes homologs or orthologs of other biological species. hsa-miR-7977 The gene was identified by Velthut-Meikas A et al., 2013, in Mol Endocri. It can be obtained by the method described in nol, online edition. Also, "hsa- "miR-7977" has a hairpin-like structure as its precursor, "hsa-mir-79 77" (miRBase Accession No. MI0025753, Sequence ID 2) 85) is known.

[0113] The "hsa-miR-6749-5p gene" or "hsa-mi" used herein The term "R-6749-5p" refers to the nucleotide sequence represented by Sequence ID No. 71, which is hsa- miR-6749-5p gene (miRBase Accession No. MIMA) This includes T0027398) and other homologs or orthologs of biological species. The -miR-6749-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6749-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6749" (miRBase Accession No. MI00 (22594, Sequence ID 286) is known.

[0114] The term "hsa-miR-1914-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-1914-3p" refers to the nucleotide sequence represented by sequence number 72, which is hsa- miR-1914-3p gene (miRBase Accession No. MIMA) This includes T0007890 and other species homologs or orthologs. The -miR-1914-3p gene was identified by Bar M et al., 2008, in Stem Cells. It can be obtained by the method described in Volume 26, pp. 2496-2505. "hsa-miR-1914-3p" is a precursor of "hsa" which has a hairpin-like structure. -mir-1914” (miRBase Accession No. MI000833 5. Sequence ID 287 is known.

[0115] The term "hsa-miR-4651 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4651 gene" used in this specification. The term "651" refers to the nucleotide sequence hsa-miR-46 represented by sequence number 73. 51 genes (miRBase Accession No. MIMAT0019715) This includes homologs or orthologs of other biological species. hsa-miR-4651 Genes are described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 78. It can be obtained by the method described in -86. Also, "hsa-miR-4651 " is a precursor that takes the hairpin-like structure of "hsa-mir-4651" (miRB The known sequence number (Accession No. MI0017279, Sequence ID 288) is Yes, they are.

[0116] The "hsa-miR-4695-5p gene" or "hsa-mi" used herein The term "R-4695-5p" refers to the nucleotide sequence represented by sequence number 74, which is hsa- miR-4695-5p gene (miRBase Accession No. MIMA) This includes T0019788) and other species homologs or orthologs. The -miR-4695-5p gene was identified by Persson H et al. in 2011 as a cancer cell. It can be obtained by the method described in Res, Vol. 71, pp. 78-86. "hsa-miR-4695-5p" is a precursor of "hsa" which has a hairpin-like structure. -mir-4695” (miRBase Accession No. MI001732 8. Sequence ID 289 is known.

[0117] The term "hsa-miR-6848-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6848-5p" refers to the nucleotide sequence represented by Sequence ID No. 75, which is hsa- miR-6848-5p gene (miRBase Accession No. MIMA) This includes T0027596 and other species homologs or orthologs. The -miR-6848-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6848-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6848" (miRBase Accession No. MI00 22694 (sequence number 290) is known.

[0118] The term "hsa-miR-1228-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-1228-3p" refers to the nucleotide sequence represented by sequence number 76, which is hsa- miR-1228-3p gene (miRBase Accession No. MIMA) This includes T0005583) and other species homologs or orthologs. The -miR-1228-3p gene was identified by Berezikov E et al., 2007, Mol It can also be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1228-3p" has a hairpin-like structure as its precursor "h sa-mir-1228” (miRBase Accession No. MI0006 318 (Sequence ID 291) is known.

[0119] The term "hsa-miR-642b-3p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-642b-3p" refers to the nucleotide sequence represented by Sequence ID No. 77, which is hsa- miR-642b-3p gene (miRBase Accession No. MIMA) This includes T0018444 and other species homologs or orthologs. The -miR-642b-3p gene was identified by Witten D et al., 2010, BMC Bio. It can be obtained by the method described in volume 8, p. 58. Also, "hsa-miR "-642b-3p" has a hairpin-like structure as its precursor, "hsa-mir-64 2b (miRBase Accession No. MI0016685, Sequence ID 2) 92) is known.

[0120] The term "hsa-miR-6746-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-6746-5p" refers to the nucleotide sequence represented by Sequence ID No. 78, which is hsa- miR-6746-5p gene (miRBase Accession No. MIMA) This includes T0027392) and other homologs or orthologs of biological species. The -miR-6746-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6746-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-6746" (miRBase Accession No. MI00 (22591, Sequence ID 293) is known.

[0121] The "hsa-miR-3620-5p gene" or "hsa-mi" used herein The term "R-3620-5p" refers to the nucleotide sequence represented by sequence number 79, which is hsa- miR-3620-5p gene (miRBase Accession No. MIMA) This includes T0022967) and other species homologs or orthologs. The -miR-3620-5p gene was identified by Witten D et al., 2010, BMC Bio. It can be obtained by the method described in volume 8, p. 58. Also, "hsa-miR "-3620-5p" has a hairpin-like structure as its precursor, "hsa-mir-36 20 (miR Base Accession No. MI0016011, Sequence ID 2) 94) is known.

[0122] The term "hsa-miR-3131 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3131 gene" or "hsa-miR-3 The term "131" refers to the nucleotide sequence hsa-miR-31 represented by sequence number 80. 31 genes (miRBase Accession No. MIMAT0014996) This includes homologs or orthologs of other biological species. hsa-miR-3131 The gene was described in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. It can be obtained by the method described. Also, "hsa-miR-3131" is "hsa-mir-3131" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0014151 (Sequence No. 295) is known.

[0123] The "hsa-miR-6732-5p gene" or "hsa-mi" used herein The term "R-6732-5p" refers to the nucleotide sequence represented by sequence number 81, which is hsa- miR-6732-5p gene (miRBase Accession No. MIMA) This includes T0027365 and other species homologs or orthologs. The -miR-6732-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6732-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6732" (miRBase Accession No. MI00 (22577, Sequence ID 296) is known.

[0124] The term "hsa-miR-7113-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-7113-3p" refers to the nucleotide sequence represented by sequence number 82, which is hsa- miR-7113-3p gene (miRBase Accession No. MIMA) This includes T0028124) and other species homologs or orthologs. -miR-7113-3p gene: Ladewig E et al., 2012, Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-7113-3p" takes on a hairpin-like structure as its precursor. "hsa-mir-7113" (miRBase Accession No. MI00 (22964, Sequence ID 297) is known.

[0125] The term "hsa-miR-23a-3p gene" or "hsa-miR" used in this specification refers to the "hsa-miR" gene. The term "-23a-3p" refers to the nucleotide sequence hsa-mi represented by sequence number 83. R-23a-3p gene (miRBase Accession No. MIMAT00) This includes 00078) and other species homologs or orthologs. The R-23a-3p gene was identified by Lagos-Quintana M et al., 2001, Sci. It can be obtained by the method described in ence, Vol. 294, pp. 853-858. Furthermore, "hsa-miR-23a-3p" has a hairpin-like structure as its precursor. sa-mir-23a” (miRBase Accession No. MI00000 79 (Sequence ID 298) is known.

[0126] The term "hsa-miR-3154 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3154 gene" or "hsa-miR-3 The term "154" refers to the nucleotide sequence hsa-miR-31 represented by sequence number 84. 54 genes (miRBase Accession No. MIMAT0015028) This includes homologs or orthologs of other biological species. hsa-miR-3154 The gene was identified by Berezikov E et al., 2006, Genome Res, Vol. 16, p. It can be obtained by the method described in 1289-1298. Also, "hsa-mi "R-3154" has a hairpin-like structure as its precursor, "hsa-mir-3154" (miRBase Accession No. MI0014182, Sequence ID 299) ) is known.

[0127] The term "hsa-miR-4723-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-4723-5p" refers to the nucleotide sequence represented by Sequence ID No. 85, which is hsa- miR-4723-5p gene (miRBase Accession No. MIMA) This includes T0019838 and other species homologs or orthologs. -miR-4723-5p gene, Persson H et al., 2011, Cancer It can be obtained by the method described in Res, Vol. 71, pp. 78-86. "hsa-miR-4723-5p" is a precursor that takes on a hairpin-like structure. -mir-4723” (miRBase Accession No. MI001735 9. Sequence ID 300 is known.

[0128] The "hsa-miR-3663-3p gene" or "hsa-mi" used in this specification The term "R-3663-3p" refers to the nucleotide sequence represented by sequence number 86, which is hsa- miR-3663-3p gene (miRBase Accession No. MIMA) This includes T0018085 and other species homologs or orthologs. The -miR-3663-3p gene was identified by Liao JY et al., 2010, PLoS One. It can be obtained by the method described in Volume 5, e10563. Also, "hsa-m "iR-3663-3p" has a hairpin-like structure as its precursor, "hsa-mir- 3663 (miRBase Accession No. MI0016064, Sequence Number) No. 301 is known.

[0129] The term "hsa-miR-4734 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "734" refers to the nucleotide sequence hsa-miR-47 represented by sequence number 87. 34 genes (miRBase Accession No. MIMAT0019859) This includes homologs or orthologs of other biological species. hsa-miR-4734 Genes are described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 78. It can be obtained by the method described in -86. Also, "hsa-miR-4734 " is a precursor that takes the hairpin-like structure of "hsa-mir-4734" (miRB The known sequence number (Accession No. MI0017371, Sequence ID 302) is Yes, they are.

[0130] The term "hsa-miR-6816-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6816-5p" refers to the nucleotide sequence represented by sequence number 88, which is hsa- miR-6816-5p gene (miRBase Accession No. MIMA) This includes T0027532) and other species homologs or orthologs. The -miR-6816-5p gene was identified by Ladewig E et al. in 2012 in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6816-5p" adopts a hairpin-like structure as its precursor. "hsa-mir-6816" (miRBase Accession No. MI00 22661 (Sequence ID 303) is known.

[0131] The term "hsa-miR-4442 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "442" refers to the nucleotide sequence hsa-miR-44, represented by sequence number 89. 42 genes (miRBase Accession No. MIMAT0018960) This includes homologs or orthologs of other biological species. hsa-miR-4442 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4442" is Its precursor is "hsa-mir-4442" (miRBase), which has a hairpin-like structure. Accession No. MI0016785 (Sequence ID 304) is known.

[0132] The term "hsa-miR-4476 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "476" refers to the nucleotide sequence hsa-miR-44, represented by SEQ ID NO: 90. 76 genes (miRBase Accession No. MIMAT0019003) This includes homologs or orthologs of other biological species. hsa-miR-4476 The gene is from Jima DD et al., 2010, Blood, Vol. 116, e118-e127. It can be obtained by the method described in [the document]. Also, "hsa-miR-4476" is Its precursor is "hsa-mir-4476" (miRBase), which has a hairpin-like structure. Accession No. MI0016828 (Sequence ID 305) is known.

[0133] The term "hsa-miR-423-5p gene" or "hsa-miR" as used herein refers to the "hsa-miR" gene used in this specification. The term "-423-5p" refers to the nucleotide sequence represented by sequence number 91, hsa-mi R-423-5p gene (miRBase Accession No. MIMAT00) This includes 04748) and other species homologs or orthologs. The R-423-5p gene was identified by Kasashima K et al. in 2004 in Biochem. The method described in Biophys Res Commun, Vol. 322, pp. 403-410. It can be obtained by [method]. Also, "hsa-miR-423-5p" is its precursor and The "hsa-mir-423" (miRBase Access) adopts a hairpin-like structure. Ion No. MI0001445 (Sequence ID 306) is known.

[0134] The term "hsa-miR-1249 gene" or "hsa-miR-1" as used herein refers to the "hsa-miR-1249 gene" used herein. The term "249" refers to the nucleotide sequence hsa-miR-12 represented by sequence number 92. 49 genes (miRBase Accession No. MIMAT0005901) This includes homologs or orthologs of other biological species. hsa-miR-1249 The gene is Morin RD et al., 2008, Genome Res, Vol. 18, p. 610. It can be obtained by the method described in -621. Also, "hsa-miR-124 "9" is "hsa-mir-1249" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0006384 (Sequence ID 307) is known. It is.

[0135] The "hsa-miR-6515-3p gene" or "hsa-mi" used herein The term "R-6515-3p" refers to the nucleotide sequence represented by sequence number 93, which is hsa- miR-6515-3p gene (miRBase Accession No. MIMA) This includes T0025487) and other species homologs or orthologs. The -miR-6515-3p gene was identified by Joyce CE et al., 2011, in Hum Mol. It can be obtained by the method described in Genet, Vol. 20, pp. 4025-4040. Furthermore, "hsa-miR-6515-3p" has a hairpin-like structure as its precursor. To get "hsa-mir-6515" (miRBase Accession No. MI 0022227 (Sequence ID 308) is known.

[0136] The term "hsa-miR-887-3p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-887-3p" refers to the hsa-mi nucleotide sequence represented by sequence number 94. R-887-3p gene (miRBase Accession No. MIMAT00) This includes 04951) and other species homologs or orthologs. The R-887-3p gene was identified by Berezikov E et al. in 2006 in Genome R. It can be obtained by the method described in es, Vol. 16, pp. 1289-1298. "hsa-miR-887-3p" has a hairpin-like structure as its precursor, "hs a-mir-887” (miRBase Accession No. MI000556 2. Sequence ID 309 is known.

[0137] The term "hsa-miR-4741 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "741" refers to the nucleotide sequence hsa-miR-47 represented by sequence number 95. 41 genes (miRBase Accession No. MIMAT0019871) This includes homologs or orthologs of other biological species. hsa-miR-4741 Genes are described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 78. It can be obtained by the method described in -86. Also, "hsa-miR-4741 " is a precursor that takes the hairpin-like structure of "hsa-mir-4741" (miRB The known sequence number (Accession No. MI0017379, Sequence ID 310) is Yes, they are.

[0138] The "hsa-miR-6766-3p gene" or "hsa-mi" used in this specification The term "R-6766-3p" refers to the nucleotide sequence represented by sequence number 96, which is hsa- miR-6766-3p gene (miRBase Accession No. MIMA) This includes T0027433) and other species homologs or orthologs. -miR-6766-3p gene: Ladewig E et al., 2012, Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6766-3p" adopts a hairpin-like structure as its precursor. "hsa-mir-6766" (miRBase Accession No. MI00 (22611, Sequence ID 311) is known.

[0139] The term "hsa-miR-4673 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "673" refers to the nucleotide sequence hsa-miR-46 represented by sequence number 97. 73 genes (miRBase Accession No. MIMAT0019755) This includes homologs or orthologs of other biological species. hsa-miR-4673 Genes are described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 78. It can be obtained by the method described in -86. Also, "hsa-miR-4673 " is a precursor that takes the hairpin-like structure of "hsa-mir-4673" (miRB The known sequence number (Accession No. MI0017304, Sequence ID 312) is Yes, they are.

[0140] The "hsa-miR-6779-5p gene" or "hsa-mi" used herein The term "R-6779-5p" refers to the hsa- nucleotide sequence represented by sequence number 98. miR-6779-5p gene (miRBase Accession No. MIMA) This includes T0027458) and other species homologs or orthologs. The -miR-6779-5p gene was identified by Ladewig E et al., 2012, in Genome It can be obtained by the method described in Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6779-5p" takes on a hairpin-like structure as its precursor. "hsa-mir-6779" (miRBase Accession No. MI00 Numbers 22624 and sequence number 313 are known.

[0141] The term "hsa-miR-4706 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "706" refers to the nucleotide sequence hsa-miR-47 represented by sequence number 99. Gene 06 (miRBase Accession No. MIMAT0019806) This includes homologs or orthologs of other biological species. hsa-miR-4706 Genes are described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 78. It can be obtained by the method described in -86. Also, "hsa-miR-4706 " is "hsa-mir-4706" (miRB), which has a hairpin-like structure as its precursor. The known sequence number (Accession No. MI0017339, Sequence ID 314) is Yes, they are.

[0142] The term "hsa-miR-1268b gene" or "hsa-miR-" used herein refers to the "hsa-miR-1268b gene" used herein. The term "1268b" refers to the hsa-miR sequence represented by Sequence ID No. 100. -1268b gene (miRBase Accession No. MIMAT0018) This includes 925) and other species homologs or orthologs. The 1268b gene was identified by Jima DD et al., 2010, Blood, Vol. 116, e118. -e127 can be obtained by the method described therein. Also, "hsa-miR-12 "68b" has a hairpin-like structure as its precursor, "hsa-mir-1268b" miRBase Accession No. MI0016748, Sequence ID 315) It is known.

[0143] The "hsa-miR-4632-5p gene" or "hsa-mi" used herein The term "R-4632-5p" refers to the nucleotide sequence represented by sequence number 101, which is an HSA. -miR-4632-5p gene (miRBase Accession No. MIM) This includes AT0022977) and other species homologs or orthologs. The a-miR-4632-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4632-5p" has a hairpin-like structure as its precursor, "hs a-mir-4632” (miRBase Accession No. MI00172 59 (Sequence ID 316) is known.

[0144] The term "hsa-miR-3197 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3197 gene" or "hsa-miR-3 The term "197" refers to the hsa-miR-3 sequence represented by sequence number 102. 197 genes (miRBase Accession No. MIMAT0015082) This includes homologs or orthologs of other biological species. hsa-miR-319 The 7 genes were identified in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. It can be obtained by the method described. Also, "hsa-miR-3197" is "hsa-mir-3197" (miRBase), which takes on a hairpin-like structure as a precursor. Accession No. MI0014245 (Sequence ID 317) is known.

[0145] The "hsa-miR-6798-5p gene" or "hsa-mi" used herein The term "R-6798-5p" refers to the nucleotide sequence represented by sequence number 103. -miR-6798-5p gene (miRBase Accession No. MIM) This includes AT0027496) and other species homologs or orthologs. The a-miR-6798-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6798-5p" has a hairpin-like structure as its precursor. "hsa-mir-6798" (miRBase Accession No.MI0 022643 (Sequence ID 318) is known.

[0146] The term "hsa-miR-711 gene" or "hsa-miR-71" as used herein refers to the "hsa-miR-711 gene" used in this specification. The term "1" refers to the nucleotide sequence hsa-miR-711, represented by sequence number 104. The gene (miRBase Accession No. MIMAT0012734) and so This includes homologs or orthologs of other organisms. (hsa-miR-711 gene) This is Artzi S et al., 2008, BMC Bioinformatics, Vol. 9, p. It can be obtained by the method described in 39. Also, "hsa-miR-711" , and as its precursor, "hsa-mir-711" (miRBase), which has a hairpin-like structure. Accession No. MI0012488 (Sequence ID 319) is known.

[0147] The term "hsa-miR-6840-3p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6840-3p" refers to the nucleotide sequence represented by sequence number 105. -miR-6840-3p gene (miRBase Accession No. MIM) This includes AT0027583) and other species homologs or orthologs. The a-miR-6840-3p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6840-3p" has a hairpin-like structure as its precursor. "hsa-mir-6840" (miRBase Accession No.MI0 022686 (sequence number 320) is known.

[0148] The "hsa-miR-6763-5p gene" or "hsa-mi" used in this specification The term "R-6763-5p" refers to the nucleotide sequence represented by sequence number 106. -miR-6763-5p gene (miRBase Accession No. MIM) This includes AT0027426) and other species homologs or orthologs. The a-miR-6763-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6763-5p" has a hairpin-like structure as its precursor. "hsa-mir-6763" (miRBase Accession No.MI0 022608 (Sequence ID 321) is known.

[0149] The "hsa-miR-6727-5p gene" or "hsa-mi" used herein The term "R-6727-5p" refers to the nucleotide sequence represented by sequence number 107, which is an HSA. -miR-6727-5p gene (miRBase Accession No. MIM) This includes AT0027355) and other species homologs or orthologs. The a-miR-6727-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6727-5p" has a hairpin-like structure as its precursor. "hsa-mir-6727" (miRBase Accession No.MI0 (022572, Sequence ID 322) is known.

[0150] The term "hsa-miR-371a-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-371a-5p" refers to the nucleotide sequence represented by sequence number 108, which is an HSA. -miR-371a-5p gene (miRBase Accession No. MIM) This includes AT0004687) and other species homologs or orthologs. The a-miR-371a-5p gene was identified by Suh MR et al., 2004, Dev Biol. It can be obtained by the method described in volume 270, pp. 488-498. Also, "h "sa-miR-371a-5p" has a hairpin-like structure as its precursor, "hsa- mir-371a” (miRBase Accession No. MI0000779 (Sequence ID 323) is known.

[0151] The term "hsa-miR-6824-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6824-5p" refers to the nucleotide sequence represented by sequence number 109, which is an HSA. -miR-6824-5p gene (miRBase Accession No. MIM) This includes AT0027548) and other species homologs or orthologs. The a-miR-6824-5p gene was identified by Ladewig E et al. in 2012, Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6824-5p" has a hairpin-like structure as its precursor. "hsa-mir-6824" (miRBase Accession No.MI0 022669 (sequence number 324) is known.

[0152] The term "hsa-miR-4648 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "648" refers to the hsa-miR-4 sequence represented by sequence number 110. 648 genes (miRBase Accession No. MIMAT0019710) This includes homologs or orthologs of other biological species. hsa-miR-464 The 8 genes were identified by Persson H et al., 2011, Cancer Res, Vol. 71, p. 7. It can be obtained by the method described in 8-86. Also, "hsa-miR-464 "8" is "hsa-mir-4648" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0017275 (Sequence ID 325) is known. It is.

[0153] The term "hsa-miR-1227-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-1227-5p" refers to the nucleotide sequence represented by sequence number 111, which is an HSA. -miR-1227-5p gene (miRBase Accession No. MIM) This includes AT0022941) and other species homologs or orthologs. The a-miR-1227-5p gene was identified by Berezikov E et al., 2007, in Mol. It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. Furthermore, "hsa-miR-1227-5p" has a hairpin-like structure as its precursor. hsa-mir-1227” (miRBase Accession No. MI000 6316 (sequence number 326) is known.

[0154] The term "hsa-miR-564 gene" or "hsa-miR-564 gene" used herein refers to the "hsa-miR-564 gene" used herein. The term "4" refers to the nucleotide sequence hsa-miR-564, represented by sequence number 112. The gene (miRBase Accession No. MIMAT0003228) and so This includes homologs or orthologs of other organisms. (hsa-miR-564 gene) This was published by Cummins JM et al. in 2006 in Proc Natl Acad Sci U. It can be obtained by the method described in SA, Vol. 103, pp. 3687-3692. Furthermore, "hsa-miR-564" has a hairpin-like structure as its precursor, "hs a-mir-564” (miRBase Accession No. MI000357 0 (sequence number 327) is known.

[0155] The "hsa-miR-3679-3p gene" or "hsa-mi" used in this specification The term "R-3679-3p" refers to the nucleotide sequence represented by sequence number 113. -miR-3679-3p gene (miRBase Accession No. MIM) This includes AT0018105) and other species homologs or orthologs. The a-miR-3679-3p gene was identified by Creighton CJ et al., 2010, PL. It can be obtained by the method described in oS One, Vol. 5, e9637. "hsa-miR-3679-3p" is a precursor that takes on a hairpin-like structure. -mir-3679” (miRBase Accession No. MI001608 0 (sequence number 279) is known.

[0156] The term "hsa-miR-2861 gene" or "hsa-miR-2" as used herein refers to the "hsa-miR-2" gene. The term "861" refers to the hsa-miR-2 sequence represented by sequence number 114. 861 gene (miRBase Accession No. MIMAT0013802) This includes homologs or orthologs of other biological species. hsa-miR-286 One gene was identified by Li H et al., 2009, J Clin Invest, Vol. 119, p. 36. It can be obtained by the method described in 66-3677. Also, "hsa-miR- "2861" is a precursor to "hsa-mir-2861", which has a hairpin-like structure. miRBase Accession No. MI0013006, Sequence ID 328) It is known.

[0157] The "hsa-miR-6737-5p gene" or "hsa-mi" used herein The term "R-6737-5p" refers to the nucleotide sequence represented by sequence number 115. -miR-6737-5p gene (miRBase Accession No. MIM) This includes AT0027375) and other species homologs or orthologs. The a-miR-6737-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6737-5p" has a hairpin-like structure as its precursor. "hsa-mir-6737" (miRBase Accession No.MI0 (022582, Sequence ID 329) is known.

[0158] The term "hsa-miR-575 gene" or "hsa-miR-57" as used herein refers to the "hsa-miR-575 gene" used herein. The term "5" refers to the nucleotide sequence hsa-miR-575, represented by sequence number 116. The gene (miRBase Accession No. MIMAT0003240) and so This includes homologs or orthologs of other organisms. (hsa-miR-575 gene) This was published by Cummins JM et al. in 2006 in Proc Natl Acad Sci U. It can be obtained by the method described in SA, Vol. 103, pp. 3687-3692. Furthermore, "hsa-miR-575" has a hairpin-like structure as its precursor, "hs a-mir-575” (miRBase Accession No. MI000358 2. Sequence ID 330 is known.

[0159] The term "hsa-miR-4725-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-4725-3p" refers to the nucleotide sequence represented by sequence number 117 of the HSA -miR-4725-3p gene (miRBase Accession No. MIM) This includes AT0019844) and other species homologs or orthologs. The a-miR-4725-3p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4725-3p" has a hairpin-like structure as its precursor, "hs a-mir-4725” (miRBase Accession No. MI00173 62 (Sequence ID 331) is known.

[0160] The term "hsa-miR-6716-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6716-5p" refers to the nucleotide sequence represented by sequence number 118. -miR-6716-5p gene (miRBase Accession No. MIM) This includes AT0025844) and other species homologs or orthologs. The a-miR-6716-5p gene was identified by Li Y et al., 2012, Gene, Vol. 497. It can be obtained by the method described on pages 330-335. Also, "hsa-miR "-6716-5p" has a hairpin-like structure as its precursor, "hsa-mir-67 16" (miRBase Accession No. MI0022550, Sequence ID 3) 32) is known.

[0161] The term "hsa-miR-4675 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "675" refers to the hsa-miR-4 sequence represented by sequence number 119. 675 gene (miRBase Accession No. MIMAT0019757) This includes homologs or orthologs of other biological species. hsa-miR-467 The five genes were identified by Persson H et al., 2011, Cancer Res, Vol. 71, p. 7. It can be obtained by the method described in 8-86. Also, "hsa-miR-467 "5" is "hsa-mir-4675" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0017306 (Sequence ID 333) is known. It is.

[0162] The term "hsa-miR-1915-3p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-1915-3p" refers to the nucleotide sequence represented by sequence number 120, which is an HSA. -miR-1915-3p gene (miRBase Accession No. MIM) This includes AT0007892) and other species homologs or orthologs. The a-miR-1915-3p gene was identified by Bar M et al. in 2008 in Stem Cells. It can be obtained by the method described in volume 26, pp. 2496-2505. Also, "hsa-miR-1915-3p" has a hairpin-like structure as its precursor, "hs a-mir-1915” (miRBase Accession No. MI00083 Number 36 (Sequence ID 334) is known.

[0163] The term "hsa-miR-671-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-671-5p" refers to the hsa-m nucleotide sequence represented by sequence number 121. iR-671-5p gene (miRBase Accession No. MIMAT0) This includes 003880) and other species homologs or orthologs. hsa-m The iR-671-5p gene was identified by Berezikov E et al., 2006, in Genome It can be obtained by the method described in Res, Vol. 16, pp. 1289-1298. Furthermore, "hsa-miR-671-5p" has a hairpin-like structure as its precursor. sa-mir-671” (miRBase Accession No. MI00037 Number 60 (Sequence ID 335) is known.

[0164] The term "hsa-miR-3656 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3656 gene" or "hsa-miR-3 The term "656" refers to the hsa-miR-3 sequence represented by sequence number 122. 656 gene (miRBase Accession No. MIMAT0018076) This includes homologs or orthologs of other biological species. hsa-miR-365 The six genes were identified by Meiri E et al., 2010, Nucleic Acids Res, 3 It can be obtained by the method described in Volume 8, pp. 6234-6246. Also, "hs a-miR-3656 has a hairpin-like structure as its precursor, hsa-mir- 3656 (miRBase Accession No. MI0016056, Array No.) Number 336 is known.

[0165] The term "hsa-miR-6722-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6722-3p" refers to the nucleotide sequence represented by sequence number 123. -miR-6722-3p gene (miRBase Accession No. MIM) This includes AT0025854) and other species homologs or orthologs. The a-miR-6722-3p gene was identified by Li Y et al., 2012, Gene, Vol. 497. It can be obtained by the method described on pages 330-335. Also, "hsa-miR "-6722-3p" has a hairpin-like structure as its precursor, "hsa-mir-67 22" (miRBase Accession No. MI0022557, Sequence ID 3) 37) is known.

[0166] The term "hsa-miR-4707-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-4707-5p" refers to the nucleotide sequence represented by sequence number 124. -miR-4707-5p gene (miRBase Accession No. MIM) This includes AT0019807) and other species homologs or orthologs. The a-miR-4707-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4707-5p" has a hairpin-like structure as its precursor, "hs a-mir-4707” (miRBase Accession No. MI00173 Number 40 (Sequence ID 338) is known.

[0167] The term "hsa-miR-4449 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "449" refers to the hsa-miR-4 sequence represented by sequence number 125. 449 gene (miRBase Accession No. MIMAT0018968) This includes homologs or orthologs of other biological species. hsa-miR-444 The 9 genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4449" , and as its precursor, "hsa-mir-4449" (miRBas) takes on a hairpin-like structure. Accession No. MI0016792 (Sequence ID 339) is known. .

[0168] The term "hsa-miR-1202 gene" or "hsa-miR-1" as used herein refers to the "hsa-miR-1202 gene" used herein. The term "202" refers to the nucleotide sequence represented by Sequence ID No. 126, hsa-miR-1 Gene 202 (miRBase Accession No. MIMAT0005865) This includes homologs or orthologs of other biological species, etc. hsa-miR-120 The two genes were identified by Marton S et al., 2008, Leukemia, Vol. 22, p. 330- It can be obtained by the method described in 338. Also, "hsa-miR-1202 " is a precursor that takes the hairpin-like structure of "hsa-mir-1202" (miRB The known accession number (MI0006334, sequence number 340) is Yes, they are.

[0169] The term "hsa-miR-4649-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-4649-5p" refers to the nucleotide sequence represented by sequence number 127, which is an HSA. -miR-4649-5p gene (miRBase Accession No. MIM) This includes AT0019711) and other species homologs or orthologs. The a-miR-4649-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4649-5p" has a hairpin-like structure as its precursor, "hs a-mir-4649” (miRBase Accession No. MI00172 76 (Sequence ID 341) is known.

[0170] The term "hsa-miR-744-5p gene" or "hsa-miR" as used herein refers to the "hsa-miR" gene used in this specification. The term "-744-5p" refers to the hsa-m nucleotide sequence represented by sequence number 128. iR-744-5p gene (miRBase Accession No. MIMAT0) This includes 004945) and other species homologs or orthologs. hsa-m The iR-744-5p gene was identified by Berezikov E et al. in 2006 in Genome It can be obtained by the method described in Res, Vol. 16, pp. 1289-1298. Furthermore, "hsa-miR-744-5p" has a hairpin-like structure as its precursor. sa-mir-744” (miRBase Accession No. MI00055 59 (Sequence ID 342) is known.

[0171] The term "hsa-miR-642a-3p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-642a-3p" refers to the nucleotide sequence represented by sequence number 129, which is an HSA. -miR-642a-3p gene (miRBase Accession No. MIM) This includes AT0020924) and other species homologs or orthologs. The a-miR-642a-3p gene was identified by Cummins JM et al. in 2006, Proc. This is described in Natl Acad Sci USA, Vol. 103, pp. 3687-3692. It can be obtained by the following method. Also, "hsa-miR-642a-3p" is "hsa-mir-642a" (miRBase), which has a hairpin-like structure as a precursor. Accession No. MI0003657 (Sequence ID 343) is known.

[0172] The terms "hsa-miR-451a gene" or "hsa-miR-4" used herein refer to the "hsa-miR-4" gene used in this specification. The term "51a" refers to the hsa-miR-4 sequence represented by sequence number 130. 51a gene (miRBase Accession No. MIMAT0001631) This includes homologs or orthologs of other biological species. hsa-miR-451 The a gene was identified by Altuvia Y et al., 2005, in Nucleic Acids Res It can be obtained by the method described in Volume 33, pp. 2697-2706. "hsa-miR-451a" has a hairpin-like structure as its precursor, "hsa-mi r-451a” (miRBase Accession No. MI0001729, Column number 344) is known.

[0173] The term "hsa-miR-6870-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6870-5p" refers to the nucleotide sequence represented by sequence number 131, which is an HSA. -miR-6870-5p gene (miRBase Accession No. MIM) This includes AT0027640) and other species homologs or orthologs. The a-miR-6870-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6870-5p" has a hairpin-like structure as its precursor. "hsa-mir-6870" (miRBase Accession No.MI0 The code 022717 (sequence number 345) is known.

[0174] The term "hsa-miR-4443 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "443" refers to the hsa-miR-4 sequence represented by sequence number 132. 443 genes (miRBase Accession No. MIMAT0018961) This includes homologs or orthologs of other biological species. hsa-miR-444 The three genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4443" , and as its precursor, "hsa-mir-4443" (miRBas) takes on a hairpin-like structure. Accession No. MI0016786 (Sequence ID 346) is known. .

[0175] The term "hsa-miR-6808-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6808-5p" refers to the nucleotide sequence represented by sequence number 133. -miR-6808-5p gene (miRBase Accession No. MIM) This includes AT0027516) and other species homologs or orthologs. The a-miR-6808-5p gene was identified by Ladewig E et al. in 2012 in Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6808-5p" has a hairpin-like structure as its precursor. "hsa-mir-6808" (miRBase Accession No.MI0 (022653, Sequence ID 347) is known.

[0176] The term "hsa-miR-4728-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-4728-5p" refers to the nucleotide sequence represented by sequence number 134. -miR-4728-5p gene (miRBase Accession No. MIM) This includes AT0019849) and other species homologs or orthologs. The a-miR-4728-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4728-5p" has a hairpin-like structure as its precursor, "hs a-mir-4728” (miRBase Accession No. MI00173 65 (Sequence ID 348) is known.

[0177] The term "hsa-miR-937-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-937-5p" refers to the hsa-m nucleotide sequence represented by sequence number 135. iR-937-5p gene (miRBase Accession No. MIMAT0) This includes 022938) and other species homologs or orthologs. hsa-m The iR-937-5p gene was identified by Lui WO et al., 2007, Cancer Res, 6. It can be obtained by the method described in Volume 7, pp. 6031-6043. Also, "hs "a-miR-937-5p" has a hairpin-like structure as its precursor, "hsa-mi r-937 (miRBase Accession No. MI0005759, array) Number 349 is known.

[0178] The term "hsa-miR-135a-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-135a-3p" refers to the nucleotide sequence represented by sequence number 136. -miR-135a-3p gene (miRBase Accession No. MIM) This includes AT0004595) and other species homologs or orthologs. The a-miR-135a-3p gene was identified by Lagos-Quintana M et al., 2002. Obtained by the method described in Curr Biol, Vol. 12, pp. 735-739. This can be done. Also, "hsa-miR-135a-3p" has a hairpin-like precursor. The structure is "hsa-mir-135a-1" (miRBase Accession No.MI0000452 (Sequence ID 350) is known.

[0179] The "hsa-miR-663b gene" or "hsa-miR-6" used in this specification The term "63b" refers to the hsa-miR-6 sequence represented by Sequence ID No. 137. 63b gene (miRBase Accession No. MIMAT0005867) This includes homologs or orthologs of other biological species. hsa-miR-663 The b gene is described in Takada S et al., 2008, Leukemia, Vol. 22, p. 1274. It can be obtained by the method described in -1278. Also, "hsa-miR-66 "3b" is a precursor that takes the hairpin-like structure of "hsa-mir-663b" (mi RBase Accession No. MI0006336 (Sequence ID 351) is known. It is being done.

[0180] The term "hsa-miR-1343-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-1343-5p" refers to the nucleotide sequence represented by sequence number 138. -miR-1343-5p gene (miRBase Accession No. MIM) This includes AT0027038) and other species homologs or orthologs. The a-miR-1343-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-1343-5p" has a hairpin-like structure as its precursor, "hs a-mir-1343” (miRBase Accession No. MI00173 Number 20 (Sequence ID 223) is known.

[0181] The term "hsa-miR-6822-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6822-5p" refers to the nucleotide sequence represented by sequence number 139, which is an HSA. -miR-6822-5p gene (miRBase Accession No. MIM) This includes AT0027544) and other species homologs or orthologs. The a-miR-6822-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6822-5p" has a hairpin-like structure as its precursor. "hsa-mir-6822" (miRBase Accession No.MI0 022667 (sequence number 352) is known.

[0182] The term "hsa-miR-6803-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-6803-5p" refers to the nucleotide sequence represented by sequence number 140, which is an HSA. -miR-6803-5p gene (miRBase Accession No. MIM) This includes AT0027506) and other species homologs or orthologs. The a-miR-6803-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6803-5p" has a hairpin-like structure as its precursor. "hsa-mir-6803" (miRBase Accession No.MI0 022648 (Sequence ID 353) is known.

[0183] The "hsa-miR-6805-3p gene" or "hsa-mi" used herein The term "R-6805-3p" refers to the nucleotide sequence represented by sequence number 141. -miR-6805-3p gene (miRBase Accession No. MIM) This includes AT0027511) and other species homologs or orthologs. The a-miR-6805-3p gene was identified by Ladewig E et al. in 2012, Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6805-3p" has a hairpin-like structure as its precursor. "hsa-mir-6805" (miRBase Accession No.MI0 (022650, Sequence ID 354) is known.

[0184] The terms "hsa-miR-128-2-5p gene" or "hsa-m" used herein refer to the "hsa-miR-128-2-5p gene" used herein. The term "iR-128-2-5p" refers to the base sequence represented by sequence number 142. sa-miR-128-2-5p gene (miRBase Accession No. MIMAT0031095) and other species homologs or orthologs are included. The hsa-miR-128-2-5p gene was identified by Lagos-Quintana M et al. By the method described in Curr Biol, Vol. 12, pp. 735-739, 2002 It can be obtained. Also, "hsa-miR-128-2-5p" is its precursor. "hsa-mir-128-2" (miRBase Access) has a hairpin-like structure. Ion No. MI0000727 (Sequence ID 355) is known.

[0185] The "hsa-miR-4640-5p gene" or "hsa-mi" used herein The term "R-4640-5p" refers to the nucleotide sequence represented by sequence number 143. -miR-4640-5p gene (miRBase Accession No. MIM) This includes AT0019699) and other species homologs or orthologs. The a-miR-4640-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4640-5p" has a hairpin-like structure as its precursor, "hs a-mir-4640” (miRBase Accession No. MI00172 67 (Sequence ID 356) is known.

[0186] The term "hsa-miR-1469 gene" or "hsa-miR-1" used herein refers to the "hsa-miR-1" gene used in this specification. The term "469" refers to the hsa-miR-1 nucleotide sequence represented by SEQ ID NO: 144. 469 genes (miRBase Accession No. MIMAT0007347) This includes homologs or orthologs of other biological species. hsa-miR-146 The 9 genes are listed in Kawaji H et al., 2008, BMC Genomics, Vol. 9, p. 1. It can be obtained by the method described in 57. Also, "hsa-miR-1469" Its precursor is "hsa-mir-1469" (miRBa Accession No. MI0007074 (Sequence ID 357) is known. ru.

[0187] The terms "hsa-miR-92a-2-5p gene" or "hsa-m" used herein refer to the "hsa-miR-92a-2-5p gene" used herein. The term "iR-92a-2-5p" refers to the base sequence represented by sequence number 145. sa-miR-92a-2-5p gene (miRBase Accession No. MIMAT0004508) and other species homologs or orthologs are included. The hsa-miR-92a-2-5p gene was identified by Mourelatos Z et al., 2002. Obtained by the method described in Genes Dev, Vol. 16, pp. 720-728. This can be done. Also, "hsa-miR-92a-2-5p" is a precursor to hairpin "hsa-mir-92a-2" (miRBase Accession) takes on a unique structure. No.MI0000094 (Sequence ID 358) is known.

[0188] The "hsa-miR-3940-5p gene" or "hsa-mi" used herein The term "R-3940-5p" refers to the nucleotide sequence represented by sequence number 146. -miR-3940-5p gene (miRBase Accession No. MIM) This includes AT0019229) and other species homologs or orthologs. The a-miR-3940-5p gene was identified by Liao JY et al., 2010, PLoS On It can be obtained by the method described in e, Volume 5, e10563. Also, "hsa- "miR-3940-5p" has a hairpin-like structure as its precursor, "hsa-mir -3940" (miRBase Accession No. MI0016597, array) Number 359) is known.

[0189] The terms "hsa-miR-4281 gene" or "hsa-miR-4" used herein refer to the "hsa-miR-4281 gene" used herein. The term "281" refers to the hsa-miR-4 sequence represented by sequence number 147. 281 gene (miRBase Accession No. MIMAT0016907) This includes homologs or orthologs of other biological species. hsa-miR-428 One gene was described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. It can be obtained by the method described. Also, "hsa-miR-4281" is "hsa-mir-4281" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0015885 (Sequence Number 360) is known.

[0190] The term "hsa-miR-1260b gene" or "hsa-miR-" used herein is incorrect. The term "1260b" refers to the hsa-miR sequence represented by sequence number 148. -1260b gene (miRBase Accession No. MIMAT0015) This includes 041) and other species homologs or orthologs. The 1260b gene was identified by Stark MS et al., 2010, PLoS One, Vol. 5, e9. It can be obtained by the method described in 685. Also, "hsa-miR-1260 "b" is a precursor that takes on a hairpin-like structure, "hsa-mir-1260b" (mi RBase Accession No. MI0014197 (Sequence ID 361) is known. It is being done.

[0191] The term "hsa-miR-4758-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-4758-5p" refers to the nucleotide sequence represented by sequence number 149, which is an HSA. -miR-4758-5p gene (miRBase Accession No. MIM) This includes AT0019903) and other species homologs or orthologs. The a-miR-4758-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4758-5p" has a hairpin-like structure as its precursor, "hs a-mir-4758” (miRBase Accession No. MI00173 99 (Sequence ID 362) is known.

[0192] The "hsa-miR-1915-5p gene" or "hsa-mi" used herein The term "R-1915-5p" refers to the nucleotide sequence represented by sequence number 150, which is an HSA. -miR-1915-5p gene (miRBase Accession No. MIM) This includes AT0007891) and other species homologs or orthologs. The a-miR-1915-5p gene was identified by Bar M et al. in 2008 in Stem Cells. It can be obtained by the method described in volume 26, pp. 2496-2505. Also, "hsa-miR-1915-5p" has a hairpin-like structure as its precursor, "hs a-mir-1915” (miRBase Accession No. MI00083 Number 36 (Sequence ID 334) is known.

[0193] The "hsa-miR-5001-5p gene" or "hsa-mi" used in this specification The term "R-5001-5p" refers to the nucleotide sequence represented by sequence number 151, which is an HSA. -miR-5001-5p gene (miRBase Accession No. MIM) This includes AT0021021) and other species homologs or orthologs. The a-miR-5001-5p gene was identified by Hansen TB et al. in 2011, under the RNA B It can be obtained by the method described in IOL, Vol. 8, pp. 378-383. "hsa-miR-5001-5p" is a precursor of "hsa" which has a hairpin-like structure. -mir-5001” (miRBase Accession No. MI001786 7. Sequence ID 363 is known.

[0194] The term "hsa-miR-4286 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4286 gene" used in this specification. The term "286" refers to the hsa-miR-4 sequence represented by sequence number 152. 286 genes (miRBase Accession No. MIMAT0016916) This includes homologs or orthologs of other biological species. hsa-miR-428 The six genes were described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. It can be obtained by the method described. Also, "hsa-miR-4286" is "hsa-mir-4286" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0015894 (Sequence No. 364) is known.

[0195] The term "hsa-miR-6126 gene" or "hsa-miR-6" as used herein refers to the "hsa-miR-6" gene used in this specification. The term "126" refers to the hsa-miR-6 sequence represented by sequence number 153. 126 genes (miRBase Accession No. MIMAT0024599) This includes homologs or orthologs of other biological species. hsa-miR-612 The 6 genes were identified by Smith JL et al., 2012, J Virol, Vol. 86, p. 5278- It can be obtained by the method described in 5287. Also, "hsa-miR-612 "6" is "hsa-mir-6126" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0021260 (Sequence ID 365) is known. It is.

[0196] The term "hsa-miR-6789-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6789-5p" refers to the nucleotide sequence represented by sequence number 154. -miR-6789-5p gene (miRBase Accession No. MIM) This includes AT0027478) and other species homologs or orthologs. The a-miR-6789-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6789-5p" has a hairpin-like structure as its precursor. "hsa-mir-6789" (miRBase Accession No.MI0 The sequence 022634 (sequence number 366) is known.

[0197] The term "hsa-miR-4459 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "459" refers to the hsa-miR-4 sequence represented by sequence number 155. 459 genes (miRBase Accession No. MIMAT0018981) This includes homologs or orthologs of other biological species. hsa-miR-445 The 9 genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4459" , and as its precursor, "hsa-mir-4459" (miRBas) takes on a hairpin-like structure. Accession No. MI0016805 (Sequence ID 367) is known. .

[0198] The term "hsa-miR-1268a gene" or "hsa-miR-" used herein refers to the "hsa-miR-1268a gene" used herein. The term "1268a" refers to the hsa-miR sequence represented by sequence number 156. -1268a gene (miRBase Accession No. MIMAT0005) This includes 922) and other species homologs or orthologs. The 1268a gene was identified by Morin RD et al., 2008, Genome Res, Vol. 18. It can be obtained by the method described on pages 610-621. Also, "hsa-mi "R-1268a" has a hairpin-like structure as its precursor, "hsa-mir-126 8a (miRBase Accession No. MI0006405, Sequence ID 3) 68) is known.

[0199] The "hsa-miR-6752-5p gene" or "hsa-mi" used herein The term "R-6752-5p" refers to the nucleotide sequence represented by sequence number 157, which is an HSA. -miR-6752-5p gene (miRBase Accession No. MIM) This includes AT0027404) and other species homologs or orthologs. The a-miR-6752-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6752-5p" has a hairpin-like structure as its precursor. "hsa-mir-6752" (miRBase Accession No.MI0 The code 022597 (sequence number 369) is known.

[0200] The term "hsa-miR-6131 gene" or "hsa-miR-6" used in this specification refers to the "hsa-miR-6" gene. The term "131" refers to the hsa-miR-6 sequence represented by sequence number 158. 131 genes (miRBase Accession No. MIMAT0024615) This includes homologs or orthologs of other biological species. hsa-miR-613 One gene was identified by Dannemann M et al., 2012, Genome Biol Evo. It can be obtained by the method described in volume 4, pp. 552-564. Also, "hs a-miR-6131 has a hairpin-like structure as its precursor, hsa-mir- 6131 (miRBase Accession No. MI0021276, Sequence Number) No. 370 is known.

[0201] The term "hsa-miR-6800-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6800-5p" refers to the nucleotide sequence represented by sequence number 159 of the HSA -miR-6800-5p gene (miRBase Accession No. MIM) This includes AT0027500) and other species homologs or orthologs. The a-miR-6800-5p gene was identified by Ladewig E et al. in 2012, in the Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6800-5p" has a hairpin-like structure as its precursor. "hsa-mir-6800" (miRBase Accession No.MI0 The code 022645 (sequence number 371) is known.

[0202] The term "hsa-miR-4532 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene used in this specification. The term "532" refers to the hsa-miR-4 sequence represented by sequence number 160. 532 genes (miRBase Accession No. MIMAT0019071) This includes homologs or orthologs of other biological species. hsa-miR-453 The two genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4532" , and as its precursor, "hsa-mir-4532" (miRBas) takes on a hairpin-like structure. Accession No. MI0016899 (Sequence ID 372) is known. .

[0203] The term "hsa-miR-6872-3p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6872-3p" refers to the nucleotide sequence represented by sequence number 161, which is an HSA. -miR-6872-3p gene (miRBase Accession No. MIM) This includes AT0027645) and other species homologs or orthologs. The a-miR-6872-3p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6872-3p" has a hairpin-like structure as its precursor. "hsa-mir-6872" (miRBase Accession No.MI0 The code 022719 (sequence number 373) is known.

[0204] The term "hsa-miR-718 gene" or "hsa-miR-718 gene" as used herein refers to the same gene used in this specification. The term "8" refers to the nucleotide sequence hsa-miR-718, represented by sequence number 162. The gene (miRBase Accession No. MIMAT0012735) and so This includes homologs or orthologs of other organisms. (hsa-miR-718 gene) This is Artzi S et al., 2008, BMC Bioinformatics, Vol. 9, p. It can be obtained by the method described in 39. Also, "hsa-miR-718" , and as its precursor, "hsa-mir-718" (miRBase) takes on a hairpin-like structure. Accession No. MI0012489 (Sequence ID 374) is known.

[0205] The terms "hsa-miR-6769a-5p gene" or "hsa-m" used herein refer to the "hsa-miR-6769a-5p gene" used herein. The term "iR-6769a-5p" refers to the base sequence represented by sequence number 163. sa-miR-6769a-5p gene (miRBase Accession No. MIMAT0027438) and other species homologs or orthologs are included. The hsa-miR-6769a-5p gene was identified by Ladewig E et al. in 2012. Obtained by the method described in enome Res, Vol. 22, pp. 1634-1645. This can be done. Also, "hsa-miR-6769a-5p" is a precursor to hairpin "hsa-mir-6769a" (miRBase Accession) takes on a unique structure. No.MI0022614 (Sequence ID 375) is known.

[0206] The term "hsa-miR-4707-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-4707-3p" refers to the nucleotide sequence represented by sequence number 164. -miR-4707-3p gene (miRBase Accession No. MIM This includes AT0019808) and other species homologs or orthologs. The a-miR-4707-3p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4707-3p" has a hairpin-like structure as its precursor, "hs a-mir-4707” (miRBase Accession No. MI00173 Number 40 (Sequence ID 338) is known.

[0207] The "hsa-miR-6765-5p gene" or "hsa-mi" used herein The term "R-6765-5p" refers to the nucleotide sequence represented by sequence number 165. -miR-6765-5p gene (miRBase Accession No. MIM) This includes AT0027430) and other species homologs or orthologs. The a-miR-6765-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6765-5p" has a hairpin-like structure as its precursor. "hsa-mir-6765" (miRBase Accession No.MI0 (022610, Sequence ID 249) is known.

[0208] The term "hsa-miR-4739 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "739" refers to the hsa-miR-4 sequence represented by sequence number 166. 739 gene (miRBase Accession No. MIMAT0019868) This includes homologs or orthologs of other biological species. hsa-miR-473 The 9 genes were identified by Persson H et al., 2011, Cancer Res, Vol. 71, p. 7. It can be obtained by the method described in 8-86. Also, "hsa-miR-473 "9" is "hsa-mir-4739" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0017377 (Sequence ID 376) is known. It is.

[0209] The term "hsa-miR-4525 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "525" refers to the hsa-miR-4 sequence represented by sequence number 167. 525 gene (miRBase Accession No. MIMAT0019064) This includes homologs or orthologs of other biological species. hsa-miR-452 The five genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4525" , and as its precursor, "hsa-mir-4525" (miRBas) takes on a hairpin-like structure. Accession No. MI0016892, Sequence ID 377) is known. .

[0210] The term "hsa-miR-4270 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4270 gene" used herein. The term "270" refers to the hsa-miR-4 sequence represented by sequence number 168. 270 genes (miRBase Accession No. MIMAT0016900) This includes homologs or orthologs of other biological species. hsa-miR-427 The 0 gene was described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. It can be obtained by the method described. Also, "hsa-miR-4270" is "hsa-mir-4270" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0015878 (Sequence No. 378) is known.

[0211] The term "hsa-miR-4534 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "534" refers to the hsa-miR-4 sequence represented by sequence number 169. 534 genes (miRBase Accession No. MIMAT0019073) This includes homologs or orthologs of other biological species. hsa-miR-453 The four genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4534" , and as its precursor, "hsa-mir-4534" (miRBas) has a hairpin-like structure. Accession No. MI0016901 (Sequence ID 379) is known. .

[0212] The term "hsa-miR-6785-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6785-5p" refers to the nucleotide sequence represented by sequence number 170, which is an HSA. -miR-6785-5p gene (miRBase Accession No. MIM) This includes AT0027470) and other homologs or orthologs of biological species. The a-miR-6785-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6785-5p" has a hairpin-like structure as its precursor. "hsa-mir-6785" (miRBase Accession No.MI0 (022630, Sequence ID 380) is known.

[0213] The term "hsa-miR-6850-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6850-5p" refers to the nucleotide sequence represented by sequence number 171, which is an HSA. -miR-6850-5p gene (miRBase Accession No. MIM) This includes AT0027600) and other species homologs or orthologs. The a-miR-6850-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6850-5p" has a hairpin-like structure as its precursor. "hsa-mir-6850" (miRBase Accession No.MI0 The code 022696 (sequence number 381) is known.

[0214] The term "hsa-miR-4697-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-4697-5p" refers to the nucleotide sequence represented by sequence number 172, which is an HSA. -miR-4697-5p gene (miRBase Accession No. MIM) This includes AT0019791) and other species homologs or orthologs. The a-miR-4697-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4697-5p" has a hairpin-like structure as its precursor, "hs a-mir-4697” (miRBase Accession No. MI00173 30 (Sequence ID 382) is known.

[0215] The term "hsa-miR-1260a gene" or "hsa-miR-" used herein refers to the "hsa-miR-1260a gene" used herein. The term "1260a" refers to the hsa-miR sequence represented by sequence number 173. -1260a gene (miRBase Accession No. MIMAT0005) This includes 911) and other species homologs or orthologs. The 1260a gene was identified by Morin RD et al., 2008, Genome Res, Vol. 18. It can be obtained by the method described on pages 610-621. Also, "hsa-mi "R-1260a" has a hairpin-like structure as its precursor, "hsa-mir-126 0a" (miRBase Accession No. MI0006394, Sequence ID 3) 83) is known.

[0216] The term "hsa-miR-4486 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "486" refers to the hsa-miR-4 sequence represented by sequence number 174. 486 genes (miRBase Accession No. MIMAT0019020) This includes homologs or orthologs of other biological species. hsa-miR-448 The six genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4486" , and as its precursor, "hsa-mir-4486" (miRBas) has a hairpin-like structure. Accession No. MI0016847 (Sequence ID 384) is known. .

[0217] The term "hsa-miR-6880-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6880-5p" refers to the nucleotide sequence represented by sequence number 175, which is an HSA. -miR-6880-5p gene (miRBase Accession No. MIM) This includes AT0027660) and other species homologs or orthologs. The a-miR-6880-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6880-5p" has a hairpin-like structure as its precursor. "hsa-mir-6880" (miRBase Accession No.MI0 The code 022727 (sequence number 385) is known.

[0218] The term "hsa-miR-6802-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6802-5p" refers to the nucleotide sequence represented by sequence number 176. -miR-6802-5p gene (miRBase Accession No. MIM) This includes AT0027504) and other species homologs or orthologs. The a-miR-6802-5p gene was identified by Ladewig E et al. in 2012, Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6802-5p" has a hairpin-like structure as its precursor. "hsa-mir-6802" (miRBase Accession No.MI0 (022647, Sequence ID 386) is known.

[0219] The term "hsa-miR-6861-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6861-5p" refers to the nucleotide sequence represented by sequence number 177, which is an HSA. -miR-6861-5p gene (miRBase Accession No. MIM) This includes AT0027623) and other species homologs or orthologs. The a-miR-6861-5p gene was identified by Ladewig E et al. in 2012, Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6861-5p" has a hairpin-like structure as its precursor. "hsa-mir-6861" (miRBase Accession No.MI0 022708 (Sequence ID 387) is known.

[0220] The term "hsa-miR-92b-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-92b-5p" refers to the hsa-m nucleotide sequence represented by sequence number 178. iR-92b-5p gene (miRBase Accession No. MIMAT0) This includes 004792) and other species homologs or orthologs. hsa-m The iR-92b-5p gene was identified by Cummins JM et al. in 2006 at Proc Nat. The method described in Acad Sci USA, Vol. 103, pp. 3687-3692. It can be obtained by [method]. Also, "hsa-miR-92b-5p" is its precursor and The "hsa-mir-92b" (miRBase Access) has a hairpin-like structure. Ion No. MI0003560 (Sequence ID 388) is known.

[0221] The term "hsa-miR-1238-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-1238-5p" refers to the nucleotide sequence represented by sequence number 179, which is an HSA. -miR-1238-5p gene (miRBase Accession No. MIM) This includes AT0022947) and other species homologs or orthologs. The a-miR-1238-5p gene was identified by Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. Furthermore, "hsa-miR-1238-5p" has a hairpin-like structure as its precursor. hsa-mir-1238” (miRBase Accession No. MI000 6328 (Sequence ID 389) is known.

[0222] The term "hsa-miR-6851-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6851-5p" refers to the nucleotide sequence represented by sequence number 180, which is an HSA. -miR-6851-5p gene (miRBase Accession No. MIM) This includes AT0027602) and other species homologs or orthologs. The a-miR-6851-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6851-5p" has a hairpin-like structure as its precursor. "hsa-mir-6851" (miRBase Accession No.MI0 The code 022697 (sequence number 390) is known.

[0223] The term "hsa-miR-7704 gene" or "hsa-miR-7" as used herein refers to the "hsa-miR-7" gene. The term "704" refers to the hsa-miR-7 sequence represented by sequence number 181. 704 gene (miRBase Accession No. MIMAT0030019) This includes homologs or orthologs of other biological species. hsa-miR-770 The four genes were identified by Swaminathan S et al., 2013, Biochem Bioph Obtained by the method described in ys Res Commun, Vol. 434, pp. 228-234. It is possible to do so. In addition, "hsa-miR-7704" has a hairpin-like precursor. The structure is "hsa-mir-7704" (miRBase Accession No. .MI0025240 (Sequence ID 391) is known.

[0224] The term "hsa-miR-149-3p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-149-3p" refers to the hsa-m nucleotide sequence represented by sequence number 182. iR-149-3p gene (miRBase Accession No. MIMAT0) This includes 004609) and other species homologs or orthologs. hsa-m The iR-149-3p gene was identified by Lagos-Quintana M et al. in 2002. It can be obtained by the method described in rr Biol, Vol. 12, pp. 735-739. Furthermore, "hsa-miR-149-3p" takes on a hairpin-like structure as its precursor. "hsa-mir-149" (miRBase Accession No. MI000 (0478, Sequence ID 392) is known.

[0225] The term "hsa-miR-4689 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "689" refers to the hsa-miR-4 sequence represented by sequence number 183. 689 gene (miRBase Accession No. MIMAT0019778) This includes homologs or orthologs of other biological species. hsa-miR-468 The 9 genes were identified by Persson H et al., 2011, Cancer Res, Vol. 71, p. 7. It can be obtained by the method described in 8-86. Also, "hsa-miR-468 "9" is "hsa-mir-4689" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0017322 (Sequence ID 393) is known. It is.

[0226] The terms "hsa-miR-4688 gene" or "hsa-miR-4" used herein refer to the "hsa-miR-4" gene. The term "688" refers to the hsa-miR-4 sequence represented by sequence number 184. 688 gene (miRBase Accession No. MIMAT0019777) This includes homologs or orthologs of other biological species. hsa-miR-468 The 8 genes were identified by Persson H et al., 2011, Cancer Res, Vol. 71, p. 7. It can be obtained by the method described in 8-86. Also, "hsa-miR-468 "8" is "hsa-mir-4688" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0017321 (Sequence ID 394) is known. It is.

[0227] The term "hsa-miR-125a-3p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-125a-3p" refers to the nucleotide sequence represented by sequence number 185. -miR-125a-3p gene (miRBase Accession No. MIM This includes AT0004602) and other species homologs or orthologs. The a-miR-125a-3p gene was identified by Lagos-Quintana M et al., 2002. Obtained by the method described in Curr Biol, Vol. 12, pp. 735-739. This can be done. Also, "hsa-miR-125a-3p" is a hairpin-like precursor. The structure is "hsa-mir-125a" (miRBase Accession No. .MI0000469 (sequence number 395) is known.

[0228] The term "hsa-miR-23b-3p gene" or "hsa-miR" used in this specification refers to the "hsa-miR" gene. The term "-23b-3p" refers to the hsa-m nucleotide sequence represented by sequence number 186. iR-23b-3p gene (miRBase Accession No. MIMAT0) This includes 000418) and other species homologs or orthologs. hsa-m The iR-23b-3p gene was identified by Lagos-Quintana M et al. in 2002. It can be obtained by the method described in rr Biol, Vol. 12, pp. 735-739. Furthermore, "hsa-miR-23b-3p" takes on a hairpin-like structure as its precursor. "hsa-mir-23b" (miRBase Accession No. MI000 (0439, Sequence ID 396) is known.

[0229] The term "hsa-miR-614 gene" or "hsa-miR-61" used in this specification refers to the "hsa-miR-614 gene" or "hsa-miR-61 The term "4" refers to the nucleotide sequence hsa-miR-614, represented by sequence number 187. The gene (miRBase Accession No. MIMAT0003282) and so This includes homologs or orthologs of other organisms. (hsa-miR-614 gene) This was published by Cummins JM et al. in 2006 in Proc Natl Acad Sci U. It can be obtained by the method described in SA, Vol. 103, pp. 3687-3692. Furthermore, "hsa-miR-614" has a hairpin-like structure as its precursor, "hs a-mir-614” (miRBase Accession No. MI000362 7. Sequence ID 397 is known.

[0230] The term "hsa-miR-1913 gene" or "hsa-miR-1" used in this specification refers to the "hsa-miR-1913 gene" or "hsa-miR-1 The term "913" refers to the hsa-miR-1 nucleotide sequence represented by sequence number 188. Gene 913 (miRBase Accession No. MIMAT0007888) This includes homologs or orthologs of other biological species, etc. hsa-miR-191 The three genes were identified by Bar M et al., 2008, Stem Cells, Vol. 26, p. 2496- It can be obtained by the method described in 2505. Also, "hsa-miR-191 "3" is "hsa-mir-1913" (miR), which has a hairpin-like structure as its precursor. Base Accession No. MI0008334 (Sequence ID 398) is known. It is.

[0231] The term "hsa-miR-16-5p gene" or "hsa-miR-" used herein refers to the "hsa-miR-16-5p gene" used herein. The term "16-5p" refers to the hsa-miR sequence represented by sequence number 189. -16-5p gene (miRBase Accession No. MIMAT0000) This includes 069) and other species homologs or orthologs. The 16-5p gene was identified by Lagos-Quintana M et al., 2001, Scienc. It can be obtained by the method described in e, Volume 294, pp. 853-858. "hsa-miR-16-5p" has a hairpin-like structure as its precursor, "hsa-m ir-16-1, hsa-mir-16-2” (miRBase Accession (No. MI0000070, MI0000115, SEQ ID NOs. 399, 400) are known. Yes, they are.

[0232] The term "hsa-miR-675-5p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-675-5p" refers to the hsa-m nucleotide sequence represented by SEQ ID NO. 190. iR-675-5p gene (miRBase Accession No. MIMAT0) This includes 004284) and other species homologs or orthologs. hsa-m The iR-675-5p gene was identified by Cai X et al., 2007, RNA, Vol. 13, p. 313- It can be obtained by the method described in 316. Also, "hsa-miR-675- "5p" is a precursor of "hsa-mir-675" (miR), which has a hairpin-like structure. Base Accession No. MI0005416 (Sequence ID 401) is known. It is.

[0233] The term "hsa-miR-486-3p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-486-3p" refers to the hsa-m nucleotide sequence represented by sequence number 191. iR-486-3p gene (miRBase Accession No. MIMAT0) This includes 004762) and other species homologs or orthologs. hsa-m The iR-486-3p gene was identified by Fu H et al., 2005, FEBS Lett, Vol. 579. It can be obtained by the method described on pages 3849-3854. Also, "hsa- "miR-486-3p" has a hairpin-like structure as its precursor, "hsa-mir- 486, hsa-mir-486-2” (miRBase Accession No. MI0002470, MI0023622, and sequence numbers 402 and 403 are known.

[0234] The "hsa-miR-6777-5p gene" or "hsa-mi" used herein The term "R-6777-5p" refers to the nucleotide sequence represented by sequence number 192, which is an HSA. -miR-6777-5p gene (miRBase Accession No. MIM) This includes AT0027454) and other species homologs or orthologs. The a-miR-6777-5p gene was identified by Ladewig E et al. in 2012 in Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6777-5p" has a hairpin-like structure as its precursor. "hsa-mir-6777" (miRBase Accession No.MI0 022622 (Sequence ID 404) is known.

[0235] The term "hsa-miR-4497 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "497" refers to the hsa-miR-4 sequence represented by sequence number 193. 497 genes (miRBase Accession No. MIMAT0019032) This includes homologs or orthologs of other biological species. hsa-miR-449 The 7 genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e12. It can be obtained by the method described in 7. Also, "hsa-miR-4497" , and as its precursor, "hsa-mir-4497" (miRBas) takes on a hairpin-like structure. Accession No. MI0016859, Sequence ID 405 is known. .

[0236] The term "hsa-miR-296-3p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-296-3p" refers to the hsa-m nucleotide sequence represented by sequence number 194. iR-296-3p gene (miRBase Accession No. MIMAT0) This includes 004679) and other species homologs or orthologs. hsa-m The iR-296-3p gene was identified by Houbaviy HB et al., 2003, at Dev Cel. It can be obtained by the method described in volume 5, pp. 351-358. Also, "hs "a-miR-296-3p" has a hairpin-like structure as its precursor, "hsa-mi r-296 (miRBase Accession No. MI0000747, array) Number 406 is known.

[0237] The term "hsa-miR-6738-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6738-5p" refers to the nucleotide sequence represented by sequence number 195, which is an HSA. -miR-6738-5p gene (miRBase Accession No. MIM) This includes AT0027377) and other species homologs or orthologs. The a-miR-6738-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6738-5p" has a hairpin-like structure as its precursor. "hsa-mir-6738" (miRBase Accession No.MI0 022583 (Sequence ID 407) is known.

[0238] The term "hsa-miR-4731-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-4731-5p" refers to the nucleotide sequence represented by sequence number 196. -miR-4731-5p gene (miRBase Accession No. MIM) This includes AT0019853) and other species homologs or orthologs. The a-miR-4731-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4731-5p" has a hairpin-like structure as its precursor, "hs a-mir-4731” (miRBase Accession No. MI00173 68 (Sequence ID 408) is known.

[0239] The term "hsa-miR-6889-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6889-5p" refers to the nucleotide sequence represented by sequence number 197. -miR-6889-5p gene (miRBase Accession No. MIM) This includes AT0027678) and other species homologs or orthologs. The a-miR-6889-5p gene was identified by Ladewig E et al. in 2012, Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6889-5p" has a hairpin-like structure as its precursor. "hsa-mir-6889" (miRBase Accession No.MI0 The code 022736 (sequence number 409) is known.

[0240] The term "hsa-miR-6786-5p gene" or "hsa-mi" used herein refers to the same gene used in this specification. The term "R-6786-5p" refers to the nucleotide sequence represented by sequence number 198. -miR-6786-5p gene (miRBase Accession No. MIM) This includes AT0027472) and other species homologs or orthologs. The a-miR-6786-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6786-5p" has a hairpin-like structure as its precursor. "hsa-mir-6786" (miRBase Accession No.MI0 (022631, Sequence ID 410) is known.

[0241] The term "hsa-miR-92a-3p gene" or "hsa-miR" used herein refers to the "hsa-miR" gene used in this specification. The term "-92a-3p" refers to the HSA-M nucleotide sequence represented by SEQ ID NO: 199. iR-92a-3p gene (miRBase Accession No. MIMAT0) This includes 000092) and other species homologs or orthologs. hsa-m The iR-92a-3p gene was identified by Mourelatos Z et al., 2002, Genes It can be obtained by the method described in Dev, Vol. 16, pp. 720-728. Also, "hsa-miR-92a-3p" has a hairpin-like structure as its precursor "hsa -mir-92a-1, hsa-mir-92a-2” (miRBase Access Ion No. MI0000093, MI0000094, Sequence ID 411, 358) It is known.

[0242] The term "hsa-miR-4294 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4294 gene" used herein. The term "294" refers to the hsa-miR-4 sequence represented by Sequence ID No. 200. 294 genes (miRBase Accession No. MIMAT0016849) This includes homologs or orthologs of other biological species. hsa-miR-429 The four genes were described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. It can be obtained by the method described. Also, "hsa-miR-4294" is "hsa-mir-4294" (miRBase A), which takes on a hairpin-like structure as a precursor. Accession No. MI0015827 (Sequence No. 412) is known.

[0243] The term "hsa-miR-4763-3p gene" or "hsa-mi" used in this specification refers to the "hsa-miR-4763-3p gene" used herein. The term "R-4763-3p" refers to the nucleotide sequence represented by sequence number 201, which is an HSA. -miR-4763-3p gene (miRBase Accession No. MIM) This includes AT0019913) and other species homologs or orthologs. The a-miR-4763-3p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4763-3p" has a hairpin-like structure as its precursor, "hs a-mir-4763” (miRBase Accession No. MI00174 (04, Sequence ID 413) is known.

[0244] The term "hsa-miR-6076 gene" or "hsa-miR-6" as used herein refers to the "hsa-miR-6" gene. The term "076" refers to the hsa-miR-6 sequence represented by sequence number 202. Gene 076 (miRBase Accession No. MIMAT0023701) This includes homologs or orthologs of other biological species. hsa-miR-607 The six genes were identified by Voellenkle C et al., 2012, RNA, Vol. 18, pp. 472-4. It can be obtained by the method described in 84. Also, "hsa-miR-6076" Its precursor is "hsa-mir-6076" (miRBa Accession No. MI0020353, Sequence ID 414) is known. ru.

[0245] The "hsa-miR-663a gene" or "hsa-miR-6" used in this specification The term "63a" refers to the hsa-miR-6 sequence represented by Sequence ID No. 203. 63a gene (miRBase Accession No. MIMAT0003326) This includes homologs or orthologs of other biological species. hsa-miR-663 The a gene was identified by Cummins JM et al., 2006, in Proc Natl Acad S Obtained by the method described in CI USA, Vol. 103, pp. 3687-3692. This can be done. In addition, "hsa-miR-663a" has a hairpin-like structure as its precursor. To take "hsa-mir-663a" (miRBase Accession No.MI (0003672, Sequence ID 415) is known.

[0246] The term "hsa-miR-760 gene" or "hsa-miR-76" as used herein refers to the "hsa-miR-760 gene" used in this specification. The term "0" refers to the nucleotide sequence hsa-miR-760, represented by sequence number 204. The gene (miRBase Accession No. MIMAT0004957) and so This includes homologs or orthologs of other organisms. (hsa-miR-760 gene) This is from Berezikov E et al., 2006, Genome Res, Vol. 16, p. 128. It can be obtained by the method described in 9-1298. Also, "hsa-miR-7 "60" is a precursor to "hsa-mir-760" (miR), which has a hairpin-like structure. Base Accession No. MI0005567 (Sequence ID 416) is known. It is.

[0247] The "hsa-miR-4667-5p gene" or "hsa-mi" used herein The term "R-4667-5p" refers to the nucleotide sequence represented by sequence number 205. -miR-4667-5p gene (miRBase Accession No. MIM) This includes AT0019743) and other species homologs or orthologs. The a-miR-4667-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4667-5p" has a hairpin-like structure as its precursor, "hs a-mir-4667” (miRBase Accession No. MI00172 97 (Sequence ID 417) is known.

[0248] The term "hsa-miR-6090 gene" or "hsa-miR-6" as used herein refers to the "hsa-miR-6" gene used in this specification. The term "090" refers to the hsa-miR-6 sequence represented by sequence number 206. Gene 090 (miRBase Accession No. MIMAT0023715) This includes homologs or orthologs of other biological species. hsa-miR-609 The 0 gene was identified by Yoo JK et al., 2012, Stem Cells Dev, Vol. 21, p. It can be obtained by the method described in 2049-2057. Also, "hsa-mi "R-6090" has a hairpin-like structure as its precursor, "hsa-mir-6090 (miRBase Accession No. MI0020367, Sequence ID 418) ) is known.

[0249] The term "hsa-miR-4730 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "730" refers to the hsa-miR-4 sequence represented by Sequence ID No. 207. 730 genes (miRBase Accession No. MIMAT0019852) This includes homologs or orthologs of other biological species. hsa-miR-473 The 0 gene is described in Persson H et al., 2011, Cancer Res, Vol. 71, p. 7. It can be obtained by the method described in 8-86. Also, "hsa-miR-473 "0" is a precursor that takes on a hairpin-like structure, "hsa-mir-4730" (miR Base Accession No. MI0017367 (Sequence ID 419) is known. It is.

[0250] The "hsa-miR-7106-5p gene" or "hsa-mi" used herein The term "R-7106-5p" refers to the nucleotide sequence represented by sequence number 208. -miR-7106-5p gene (miRBase Accession No. MIM) This includes AT0028109) and other species homologs or orthologs. The a-miR-7106-5p gene was identified by Ladewig E et al. in 2012, in Genom It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-7106-5p" has a hairpin-like structure as its precursor. "hsa-mir-7106" (miRBase Accession No.MI0 (022957, Sequence ID 420) is known.

[0251] The term "hsa-miR-3196 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3196 gene" or "hsa-miR-3 The term "196" refers to the hsa-miR-3 sequence represented by sequence number 209. 196 genes (miRBase Accession No. MIMAT0015080) This includes homologs or orthologs of other biological species. hsa-miR-319 The six genes were identified in Stark MS et al., 2010, PLoS One, Vol. 5, e9685. It can be obtained by the method described. Also, "hsa-miR-3196" is "hsa-mir-3196" (miRBase), which has a hairpin-like structure as a precursor. Accession No. MI0014241 (Sequence ID 421) is known.

[0252] The term "hsa-miR-5698 gene" or "hsa-miR-5" as used herein refers to the "hsa-miR-5698 gene" used herein. The term "698" refers to the hsa-miR-5 sequence represented by sequence number 210. 698 gene (miRBase Accession No. MIMAT0022491) This includes homologs or orthologs of other biological species. hsa-miR-569 The 8 genes were identified by Watahiki A et al., 2011, PLoS One, Vol. 6, e249. It can be obtained by the method described in 50. Also, "hsa-miR-5698" Its precursor is "hsa-mir-5698" (miRBa Accession No. MI0019305, Sequence ID 422) is known. ru.

[0253] The term "hsa-miR-6087 gene" or "hsa-miR-6" as used herein refers to the "hsa-miR-6" gene. The term "087" refers to the hsa-miR-6 sequence represented by sequence number 211. Gene 087 (miRBase Accession No. MIMAT0023712) This includes homologs or orthologs of other biological species. hsa-miR-608 The 7 genes were identified by Yoo JK et al., 2012, Stem Cells Dev, Vol. 21, p. It can be obtained by the method described in 2049-2057. Also, "hsa-mi "R-6087" has a hairpin-like structure as its precursor, "hsa-mir-6087" (miRBase Accession No. MI0020364, Sequence ID 423) ) is known.

[0254] The "hsa-miR-4665-5p gene" or "hsa-mi" used herein The term "R-4665-5p" refers to the nucleotide sequence represented by sequence number 212. -miR-4665-5p gene (miRBase Accession No. MIM) This includes AT0019739) and other species homologs or orthologs. The a-miR-4665-5p gene was identified by Persson H et al., 2011, Cance It can be obtained by the method described in r Res, Vol. 71, pp. 78-86. Also, "hsa-miR-4665-5p" has a hairpin-like structure as its precursor, "hs a-mir-4665” (miRBase Accession No. MI00172 95 (Sequence ID 275) is known.

[0255] The term "hsa-miR-8059 gene" or "hsa-miR-8" as used herein refers to the "hsa-miR-8" gene used in this specification. The term "059" refers to the hsa-miR-8 sequence represented by sequence number 213. Gene 059 (miRBase Accession No. MIMAT0030986) This includes homologs or orthologs of other biological species. hsa-miR-805 The 9 genes are described in Wang HJ et al., 2013, Shock, Vol. 39, pp. 480-487. It can be obtained by the method described. Also, "hsa-miR-8059" is "hsa-mir-8059" (miRBase), which has a hairpin-like structure as a precursor. Accession No. MI0025895 (Sequence ID 424) is known.

[0256] The term "hsa-miR-6879-5p gene" or "hsa-mi" as used herein refers to the "hsa-mi" gene used in this specification. The term "R-6879-5p" refers to the nucleotide sequence represented by sequence number 214. -miR-6879-5p gene (miRBase Accession No. MIM) This includes AT0027658) and other species homologs or orthologs. The a-miR-6879-5p gene was identified by Ladewig E et al. in 2012 in Genom. It can be obtained by the method described in e Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6879-5p" has a hairpin-like structure as its precursor. "hsa-mir-6879" (miRBase Accession No.MI0 The code 022726 (sequence number 425) is known.

[0257] The term "hsa-miR-6717-5p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-6717-5p" refers to the nucleotide sequence represented by sequence number 666. -miR-6717-5p gene (miRBase Accession No. MIM) This includes AT0025846) and other homologs or orthologs of biological species. The sa-miR-6717-5p gene was identified by Li Y et al., 2012, Gene, Vol. 497. It can be obtained by the method described on pages 330-335. Also, "hsa-mi "R-6717-5p" has a hairpin-like structure as its precursor, "hsa-mir-6 717" (miRBase Accession No. MI0022551, Sequence ID) 677) is known.

[0258] The term "hsa-miR-3648 gene" or "hsa-miR-3" used in this specification refers to the "hsa-miR-3648 gene" or "hsa-miR-3 The term "648" refers to the hsa-miR-3 sequence represented by sequence number 667. 648 gene (miRBase Accession No. MIMAT0018068) This includes homologs or orthologs of other biological species. hsa-miR-36 48 genes were identified by Meiri E et al., 2010, Nucleic Acids Res. It can be obtained by the method described in Volume 38, pp. 6234-6246. Also, "h "sa-miR-3648" has a hairpin-like structure as its precursor, "hsa-mir -3648 (miRBase Accession No. MI0016048, array) Number 678 is known.

[0259] The "hsa-miR-3162-5p gene" or "hsa-mi" used herein The term "R-3162-5p" refers to the nucleotide sequence represented by sequence number 668. -miR-3162-5p gene (miRBase Accession No. MIM) This includes AT0015036) and other homologs or orthologs of biological species. The sa-miR-3162-5p gene was identified by Stark MS et al., 2010, PLoS. It can be obtained by the method described in One, Volume 5, e9685. Also, "hsa "-miR-3162-5p" has a hairpin-like structure as its precursor, "hsa-mi r-3162” (miRBase Accession No. MI0014192, Column number 679) is known.

[0260] The term "hsa-miR-1909-3p gene" or "hsa-mi" used herein refers to the "hsa-mi" gene used in this specification. The term "R-1909-3p" refers to the nucleotide sequence represented by sequence number 669. -miR-1909-3p gene (miRBase Accession No. MIM) This includes AT0007883) and other homologs or orthologs of biological species. The sa-miR-1909-3p gene was identified by Bar M et al., 2008, in Stem Cell. It can be obtained by the method described in LS, Vol. 26, pp. 2496-2505. "hsa-miR-1909-3p" has a hairpin-like structure as its precursor "h sa-mir-1909” (miRBase Accession No. MI0008 Numbers 330 and SEQ ID NO: 680 are known.

[0261] The term "hsa-miR-8073 gene" or "hsa-miR-8" as used herein refers to the "hsa-miR-8" gene used in this specification. The term "073" refers to the hsa-miR-8 sequence represented by sequence number 670. Gene 073 (miRBase Accession No. MIMAT0031000) This includes homologs or orthologs of other biological species, etc. hsa-miR-80 73 genes are listed in Wang HJ et al., 2013, Shock, Vol. 39, pp. 480-487. It can be obtained by the method described in [the document]. Also, "hsa-miR-8073" is Its precursor is "hsa-mir-8073" (miRBase), which has a hairpin-like structure. Accession No. MI0025909 (Sequence ID 681) is known.

[0262] The terms "hsa-miR-6769b-5p gene" or "hsa-m" used herein refer to the "hsa-miR-6769b-5p gene" used herein. The term "iR-6769b-5p" refers to the base sequence represented by sequence number 671. sa-miR-6769b-5p gene (miRBase Accession No. MIMAT0027620) and other species homologs or orthologs are included. The hsa-miR-6769b-5p gene was identified by Ladewig E et al. in 2012. It can be obtained by the method described in Genome Res, Vol. 22, pp. 1634-1645. This can be done. Also, "hsa-miR-6769b-5p" is a precursor to hair p "hsa-mir-6769b" (miRBase Accession) takes on a n-like structure. No.MI0022706 (sequence number 682) is known.

[0263] The term "hsa-miR-6836-3p gene" or "hsa-mi" used in this specification refers to the "hsa-mi" gene. The term "R-6836-3p" refers to the nucleotide sequence represented by sequence number 672. -miR-6836-3p gene (miRBase Accession No. MIM) This includes AT0027575) and other homologs or orthologs of biological species. The sa-miR-6836-3p gene was identified by Ladewig E et al., 2012, in Genetics. It can be obtained by the method described in me Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6836-3p" has a hairpin-like structure as its precursor. To get "hsa-mir-6836" (miRBase Accession No. MI 0022682 (Sequence ID 683) is known.

[0264] The term "hsa-miR-4484 gene" or "hsa-miR-4" as used herein refers to the "hsa-miR-4" gene. The term "484" refers to the hsa-miR-4 sequence represented by sequence number 673. 484 genes (miRBase Accession No. MIMAT0019018) This includes homologs or orthologs of other biological species. hsa-miR-44 84 genes were identified by Jima DD et al., 2010, Blood, Vol. 116, e118-e1. It can be obtained by the method described in 27. Also, "hsa-miR-4484" Its precursor is "hsa-mir-4484" (miRBa Accession No. MI0016845, Sequence ID 684) is known. ru.

[0265] The term "hsa-miR-6819-5p gene" or "hsa-mi" used herein refers to the same gene used in this document. The term "R-6819-5p" refers to the nucleotide sequence represented by sequence number 674. -miR-6819-5p gene (miRBase Accession No. MIM) This includes AT0027538) and other homologs or orthologs of biological species. The sa-miR-6819-5p gene was identified by Ladewig E et al., 2012, in Genetics. It can be obtained by the method described in me Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6819-5p" has a hairpin-like structure as its precursor. To get "hsa-mir-6819" (miRBase Accession No. MI 0022664 (Sequence ID 685) is known.

[0266] The "hsa-miR-6794-5p gene" or "hsa-mi" used herein The term "R-6794-5p" refers to the nucleotide sequence represented by sequence number 675. -miR-6794-5p gene (miRBase Accession No. MIM) This includes AT0027488) and other homologs or orthologs of biological species. The sa-miR-6794-5p gene was identified by Ladewig E et al., 2012, in Genetics. It can be obtained by the method described in me Res, Vol. 22, pp. 1634-1645. Furthermore, "hsa-miR-6794-5p" has a hairpin-like structure as its precursor. To get "hsa-mir-6794" (miRBase Accession No. MI 0022639 (sequence number 686) is known.

[0267] The term "hsa-miR-24-3p gene" or "hsa-miR-" used herein refers to the "hsa-miR-24-3p gene" used herein. The term "24-3p" refers to the hsa-miR sequence represented by sequence number 676. -24-3p gene (miRBase Accession No. MIMAT0000) This includes 080) and other species homologs or orthologs. hsa-miR The -24-3p gene was identified by Lagos-Quintana M et al., 2001, in Science. It can be obtained by the method described in CE, Vol. 294, pp. 853-858. Also, "hsa-miR-24-3p" has a hairpin-like structure as its precursor "hsa- mir-24-1,hsa-mir-24-2” (miRBase Accession (No. MI0000080, MI0000081, Sequence IDs 687, 688) are known. It is.

[0268] Furthermore, mature miRNAs are formed from RNA precursors that take on a hairpin-like structure. When A is excised, one to several bases before and after the sequence may be excised to be shorter or longer. Base substitutions can occur, resulting in mutants called isomiR (Morin R D. et al., 2008, Genome Res., Vol. 18, pp. 610-621). In RBase Release 20, either sequence numbers 1-214 or 666-676 In addition to the base sequence represented by , there are numerous isomiR sequences, number 426-665. Variants and fragments of the base sequence represented by any of 689-700 are also shown. These variants also have bases represented by SEQ ID NOs: 1-214 and 666-676. It can be obtained as a miRNA having a sequence.

[0269] That is, Sequence IDs 1, 2, 6, 9, 13, 18, 20, 21, 23, 28 of the present invention, 29, 30, 31, 34, 36, 40, 41, 46, 47, 50, 52, 54, 55, 5 6, 58, 64, 66, 67, 68, 72, 73, 74, 76, 77, 79, 80, 83 , 84, 85, 87, 89, 90, 91, 92, 93, 94, 95, 97, 99, 100 , 101, 102, 104, 108, 110, 112, 113, 114, 117, 118 , 120, 121, 122, 124, 125, 126, 127, 128, 129, 130 , 132, 134, 135, 136, 137, 142, 143, 145, 146, 147 , 148, 149, 150, 151, 152, 153, 155, 156, 158, 160 , 162, 164, 166, 167, 173, 174, 178, 182, 183, 184 , 185, 186, 187, 188, 189, 190, 191, 193, 194, 196 , 199, 201, 203, 204, 205, 207, 209, 210, 211, 212 A base sequence represented by 666, 667, 668, 669, 673, and 676, or the salt thereof. Among polynucleotide variants consisting of a base sequence in which u is replaced by t, for example As the longest variants registered in miRBase Release 20, each Sequence numbers 426, 428, 430, 432, 434, 436, 438, 440, 442 ,444,446,448,450,452,454,456,458,460,462 ,464,466,468,470,472,474,476,478,480,482 ,484,486,488,490,492,494,496,498,500,502 , 504, 506, 508, 510, 512, 514, 516, 518, 520, 522 , 524, 526, 528, 530, 532, 534, 536, 538, 540, 542 , 544, 546, 548, 550, 552, 554, 556, 558, 560, 562 , 564, 566, 568, 570, 572, 574, 576, 578, 580, 582 , 584, 586, 588, 590, 592, 594, 596, 598, 600, 602 , 604, 606, 608, 610, 612, 614, 616, 618, 620, 622 ,624,626,628,630,632,634,636,638,640,642 ,644,646,648,650,652,654,656,658,660,662 Polynucleotides represented by 664, 689, 691, 693, 695, 697, and 699 Examples include cydo. Also, the sequence numbers 1, 2, 6, 9, 13, 18, 20, 21 of the present invention, 23, 28, 29, 30, 31, 34, 36, 40, 41, 46, 47, 50, 52, 5 4, 55, 56, 58, 64, 66, 67, 68, 72, 73, 74, 76, 77, 79 , 80, 83, 84, 85, 87, 89, 90, 91, 92, 93, 94, 95, 97, 99, 100, 101, 102, 104, 108, 110, 112, 113, 114, 1 17, 118, 120, 121, 122, 124, 125, 126, 127, 128, 1 29, 130, 132, 134, 135, 136, 137, 142, 143, 145, 1 46, 147, 148, 149, 150, 151, 152, 153, 155, 156, 1 58, 160, 162, 164, 166, 167, 173, 174, 178, 182, 1 83, 184, 185, 186, 187, 188, 189, 190, 191, 193, 1 94, 196, 199, 201, 203, 204, 205, 207, 209, 210, 2 Base sequences represented by 11, 212, 666, 667, 668, 669, 673, and 676 or a variant of a polynucleotide consisting of a base sequence in which u is replaced by t. Among them, for example, the shortest mutant registered in miRBase Release 20. These are sequence numbers 427, 429, 431, 433, 435, 437, and 439, respectively. 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, Represented by 661, 663, 665, 690, 692, 694, 696, 698 and 700 Examples include polynucleotides with sequences. In addition to these mutants and fragments, mi The base sequences represented by sequence numbers 1-214 and 666-676 registered in RBase Numerous polynucleotides that are isomiR can be cited. Furthermore, Sequence ID No. 1~ Examples of polynucleotides containing base sequences represented by 214 and 666-676, Therefore, use one of the precursors, SEQ ID NOs. 215-425 and 677-688 respectively. Examples of polynucleotides that can be represented include polynucleotides.

[0270] The name of the gene consisting of the base sequence represented by Sequence IDs 1-700 and miRBase Ac The cession number (registration number) is listed in Table 1.

[0271] In this specification, "specifically binding" means the nucleic acid probe or protozoan used in the present invention. This means that the lymer binds to a specific target nucleic acid and is effectively unable to bind to other nucleic acids. .

[0272] [Table 1]

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[0291] This specification is based on Japanese Patent Applications No. 2014-125036 and 2015-070379, which form the basis of the priority claim of this application. It includes the contents described in the specification and / or drawings. [Effects of the Invention]

[0292] This invention makes it possible to easily and accurately detect esophageal cancer. For example, This refers to the measurement of several miRNA levels in the patient's blood, serum, and / or plasma, which can be collected minimally invasively. This serves as a guide, allowing for easy detection of whether or not a patient has esophageal cancer. [Brief explanation of the drawing]

[0293] [Figure 1] This figure shows the relationship between hsa-miR-1343-3p, which is generated from the sequence represented by sequence number 9, and hsa-miR-1343-5p, which is generated from the sequence represented by sequence number 138, both of which are derived from hsa-mir-1343, the precursor consisting of the sequence represented by sequence number 223. [Figure 2]Left figure: The vertical axis shows the measured expression levels of hsa-miR-204-3p (SEQ ID NO: 1) in healthy individuals (100 people) and esophageal cancer patients (34 people) selected as the training sample group. The horizontal line in the figure indicates the threshold (12.3) for distinguishing between the two groups, optimized by Fisher's discriminant analysis. Right figure: The vertical axis shows the measured expression levels of hsa-miR-204-3p (SEQ ID NO: 1) in healthy individuals (50 people) and esophageal cancer patients (16 people) selected as the test sample group. The horizontal line in the figure indicates the threshold (12.3) for distinguishing between the two groups, set for the training sample group. [Figure 3] Left figure: The expression levels of hsa-miR-1247-3p (SEQ ID NO: 2) on the horizontal axis and the expression levels of hsa-miR-6857-5p (SEQ ID NO: 4) on the vertical axis for the training sample group consisting of healthy individuals (100 people, circles) and esophageal cancer patients (34 people, triangles). The line in the figure shows the discriminant function (0=2.42x+y-21.17) optimized by Fisher's discriminant analysis to distinguish between the two groups. Right figure: The expression levels of hsa-miR-1247-3p (SEQ ID NO: 2) on the horizontal axis and the expression levels of hsa-miR-6857-5p (SEQ ID NO: 4) on the vertical axis for the test sample group consisting of healthy individuals (50 people, circles) and esophageal cancer patients (34 people, triangles). The line in the figure represents the threshold (0 = 2.42x + y - 21.17) set for the training sample group to distinguish between the two groups. [Figure 4]A: The learning sample group consisted of 34 esophageal cancer patients, 103 healthy individuals, 69 pancreatic cancer patients, 66 biliary tract cancer patients, 30 colorectal cancer patients, 33 gastric cancer patients, 32 liver cancer patients, and 15 patients with benign pancreatic and biliary tract diseases. The samples used were hsa-miR-204-3p (SEQ ID NO: 1), hsa-miR-6726-5p (SEQ ID NO: 5), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-1343-5p (SEQ ID NO: 138), hsa-miR-4739 (SEQ ID NO: 166), and hsa-miR- A discriminant formula was created using Fisher's discriminant analysis from the expression levels of R-6717-5p (sequence number 666) (-2.65xhsa-miR-4739―3.01xhsa-miR-1343-5p+0.69xhsa-miR-204-3p+0.95xhsa-miR-4723-5p-0.56xhsa-miR-6726-5p-0.99xhsa-miR-6717-5p+57.33). The discriminant score obtained from this formula is plotted on the vertical axis, and the sample groups are plotted on the horizontal axis. The dotted line in the figure indicates the discriminant boundary where the two groups are distinguished by a discriminant score of 0. B: This graph shows the expression levels of hsa-miR-204-3p (SEQ ID NO: 1), hsa-miR-6726-5p (SEQ ID NO: 5), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-1343-5p (SEQ ID NO: 138), hsa-miR-4739 (SEQ ID NO: 166), and hsa-miR-6717-5p (SEQ ID NO: 666) from 16 esophageal cancer patients, 47 healthy individuals, 30 pancreatic cancer patients, 33 biliary tract cancer patients, 20 colorectal cancer patients, 17 gastric cancer patients, 20 liver cancer patients, and 6 patients with benign pancreatic and biliary tract diseases, selected as the test sample group. The discriminant score obtained from the discriminant formula created for the training sample group is on the vertical axis, and the sample group is on the horizontal axis. The dotted line in the figure indicates the discriminant boundary where the discriminant score is 0, thus distinguishing between the two groups. [Modes for carrying out the invention]

[0294] The present invention will be described in more detail below. 1. Target nucleic acids for esophageal cancer Using the nucleic acid probe or primer for esophageal cancer detection as defined above in the present invention, the esophagus As an esophageal cancer marker for detecting the presence and / or absence of esophageal cancer cells The main target nucleic acids are hsa-miR-204-3p and hsa-miR-1247-3 p, hsa-miR-6875-5p, hsa-miR-6857-5p, hsa-mi R-6726-5p, hsa-miR-3188, hsa-miR-8069, hsa- miR-4257, hsa-miR-1343-3p, hsa-miR-7108-5p , hsa-miR-6825-5p, hsa-miR-7641, hsa-miR-31 85, hsa-miR-4746-3p, hsa-miR-6791-5p, hsa-m iR-6893-5p, hsa-miR-4433b-3p, hsa-miR-3135 b, hsa-miR-6781-5p, hsa-miR-1908-5p, hsa-mi R-4792, hsa-miR-7845-5p, hsa-miR-4417, hsa- miR-3184-5p, hsa-miR-1225-5p, hsa-miR-1231 , hsa-miR-1225-3p, hsa-miR-150-3p, hsa-miR- 4433-3p, hsa-miR-6125, hsa-miR-4513, hsa-mi R-6787-5p, hsa-miR-6784-5p, hsa-miR-615-5p , hsa-miR-6765-3p, hsa-miR-5572, hsa-miR-68 42-5p, hsa-miR-8063, hsa-miR-6780b-5p, hsa- miR-187-5p, hsa-miR-128-1-5p, hsa-miR-6729 -5p, hsa-miR-6741-5p, hsa-miR-6757-5p, hsa- miR-7110-5p, hsa-miR-7975, hsa-miR-1233-5p hsa-miR-6845-5p hsa-miR-3937 hsa-miR-44 67、hsa-miR-7109-5p、hsa-miR-6088、hsa-miR- 6782-5p, hsa-miR-5195-3p, hsa-miR-4454, hsa -miR-6724-5p、hsa-miR-8072、hsa-miR-4516、h sa-miR-6756-5p, hsa-miR-4665-3p, hsa-miR-6 826-5p、hsa-miR-6820-5p、hsa-miR-6887-5p、h sa-miR-3679-5p, hsa-miR-7847-3p, hsa-miR-6 721-5p、hsa-miR-3622a-5p、hsa-miR-939-5p、h sa-miR-602, hsa-miR-7977, hsa-miR-6749-5p hsa-miR-1914-3p, hsa-miR-4651, hsa-miR-469 5-5p、hsa-miR-6848-5p、hsa-miR-1228-3p、hsa -miR-642b-3p、hsa-miR-6746-5p、hsa-miR-362 0-5p、hsa-miR-3131、hsa-miR-6732-5p、hsa-mi R-7113-3p、hsa-miR-23a-3p、hsa-miR-3154、hs a-miR-4723-5p, hsa-miR-3663-3p, hsa-miR-47 34、hsa-miR-6816-5p、hsa-miR-4442、hsa-miR- 4476、hsa-miR-423-5p、hsa-miR-1249、hsa-miR -6515-3p、hsa-miR-887-3p、hsa-miR-4741、hsa -miR-6766-3p、hsa-miR-4673、hsa-miR-6779-5 p、hsa-miR-4706、hsa-miR-1268b、hsa-miR-463 2-5p、hsa-miR-3197、hsa-miR-6798-5p、hsa-mi R-711、hsa-miR-6840-3p、hsa-miR-6763-5p、hs a-miR-6727-5p、hsa-miR-371a-5p、hsa-miR-68 24-5p、hsa-miR-4648、hsa-miR-1227-5p、hsa-m iR-564、hsa-miR-3679-3p、hsa-miR-2861、hsa- miR-6737-5p、hsa-miR-4725-3p、hsa-miR-6716 -5p、hsa-miR-4675、hsa-miR-1915-3p、hsa-miR -671-5p、hsa-miR-3656、hsa-miR-6722-3p、hsa -miR-4707-5p、hsa-miR-4449、hsa-miR-1202、h sa-miR-4649-5p、hsa-miR-744-5p、hsa-miR-64 2a-3p、hsa-miR-451a、hsa-miR-6870-5p、hsa-m iR-4443、hsa-miR-6808-5p、hsa-miR-4728-5p、 hsa-miR-937-5p、hsa-miR-135a-3p、hsa-miR-6 63b、hsa-miR-1343-5p、hsa-miR-6822-5p、hsa- miR-6803-5p、hsa-miR-6805-3p、hsa-miR-128- 2-5p、hsa-miR-4640-5p、hsa-miR-1469、hsa-mi R-92a-2-5p、hsa-miR-3940-5p、hsa-miR-4281、 hsa-miR-1260b, hsa-miR-4758-5p, hsa-miR-19 15-5p、hsa-miR-5001-5p、hsa-miR-4286、hsa-m iR-6126, hsa-miR-6789-5p, hsa-miR-4459, hsa -miR-1268a、hsa-miR-6752-5p、hsa-miR-6131、 hsa-miR-6800-5p, hsa-miR-4532, hsa-miR-687 2-3p、hsa-miR-718、hsa-miR-6769a-5p、hsa-mi R-4707-3p、hsa-miR-6765-5p、hsa-miR-4739、h sa-miR-4525, hsa-miR-4270, hsa-miR-4534, hs a-miR-6785-5p, hsa-miR-6850-5p, hsa-miR-46 97-5p、hsa-miR-1260a、hsa-miR-4486、hsa-miR -6880-5p, hsa-miR-6802-5p, hsa-miR-6861-5p hsa-miR-92b-5p hsa-miR-1238-5p hsa-miR- 6851-5p, hsa-miR-7704, hsa-miR-149-3p, hsa- miR-4689、hsa-miR-4688、hsa-miR-125a-3p、hs a-miR-23b-3p、hsa-miR-614、hsa-miR-1913、hs a-miR-16-5p, hsa-miR-6717-5p, hsa-miR-3648 hsa-miR-3162-5p hsa-miR-1909-3p hsa-miR -8073、hsa-miR-6769b-5p、hsa-miR-6836-3p、h sa-miR-4484, hsa-miR-6819-5p, and hsa-miR-67 At least one miRNA selected from the group consisting of 94-5p can be used. Furthermore, other esophageal cancer markers that can be combined with these miRNAs, that is, Furthermore, miRNAs consisting of hsa-miR-575 and hsa-miR-24-3p are also targeted. It can be preferably used as nucleic acid. Furthermore, it can be combined with these miRNAs. Other esophageal cancer markers that can be used include hsa-miR-675-5p, hsa-m iR-486-3p, hsa-miR-6777-5p, hsa-miR-4497, h sa-miR-296-3p, hsa-miR-6738-5p, hsa-miR-47 31-5p, hsa-miR-6889-5p, hsa-miR-6786-5p, hs a-miR-92a-3p, hsa-miR-4294, hsa-miR-4763-3 p, hsa-miR-6076, hsa-miR-663a, hsa-miR-760, hsa-miR-4667-5p, hsa-miR-6090, hsa-miR-473 0, hsa-miR-7106-5p, hsa-miR-3196, hsa-miR-5 698, hsa-miR-6087, hsa-miR-4665-5p, hsa-miR At least one selected from the group consisting of -8059 and hsa-miR-6879-5p The miRNA can also be preferably used as a target nucleic acid.

[0295] The above miRNAs include, for example, one of the sequence numbers 1-214 and 666-676. Human genes containing the represented base sequence (i.e., hsa-miR-204-3, respectively) p、hsa-miR-1247-3p、hsa-miR-6875-5p、hsa-mi R-6857-5p、hsa-miR-6726-5p、hsa-miR-3188、h sa-miR-8069、hsa-miR-4257、hsa-miR-1343-3p hsa-miR-7108-5p hsa-miR-6825-5p hsa-miR -7641、hsa-miR-3185、hsa-miR-4746-3p、hsa-m iR-6791-5p, hsa-miR-6893-5p, hsa-miR-4433b -3p、hsa-miR-3135b、hsa-miR-6781-5p、hsa-mi R-1908-5p、hsa-miR-4792、hsa-miR-7845-5p、h sa-miR-4417, hsa-miR-3184-5p, hsa-miR-1225 -5p、hsa-miR-1231、hsa-miR-1225-3p、hsa-miR -150-3p、hsa-miR-4433-3p、hsa-miR-6125、hsa -miR-4513、hsa-miR-6787-5p、hsa-miR-6784-5 p, hsa-miR-615-5p, hsa-miR-6765-3p, hsa-miR -5572、hsa-miR-6842-5p、hsa-miR-8063、hsa-m iR-6780b-5p, hsa-miR-187-5p, hsa-miR-128-1 -5p、hsa-miR-6729-5p、hsa-miR-6741-5p、hsa- miR-6757-5p, hsa-miR-7110-5p, hsa-miR-7975 hsa-miR-1233-5p hsa-miR-6845-5p hsa-miR -3937、hsa-miR-4467、hsa-miR-7109-5p、hsa-m iR-6088, hsa-miR-6782-5p, hsa-miR-5195-3p hsa-miR-4454, hsa-miR-6724-5p, hsa-miR-807 2、hsa-miR-4516、hsa-miR-6756-5p、hsa-miR-4 665-3p、hsa-miR-6826-5p、hsa-miR-6820-5p、h sa-miR-6887-5p、hsa-miR-3679-5p、hsa-miR-7 847-3p、hsa-miR-6721-5p、hsa-miR-3622a-5p、 hsa-miR-939-5p, hsa-miR-602, hsa-miR-7977 hsa-miR-6749-5p、hsa-miR-1914-3p、hsa-miR- 4651、hsa-miR-4695-5p、hsa-miR-6848-5p、hsa -miR-1228-3p、hsa-miR-642b-3p、hsa-miR-674 6-5p、hsa-miR-3620-5p、hsa-miR-3131、hsa-mi R-6732-5p、hsa-miR-7113-3p、hsa-miR-23a-3p hsa-miR-3154 hsa-miR-4723-5p hsa-miR-36 63-3p、hsa-miR-4734、hsa-miR-6816-5p、hsa-m iR-4442, hsa-miR-4476, hsa-miR-423-5p, hsa- miR-1249, hsa-miR-6515-3p, hsa-miR-887-3p hsa-miR-4741, hsa-miR-6766-3p, hsa-miR-467 3、hsa-miR-6779-5p、hsa-miR-4706、hsa-miR-1 268b, hsa-miR-4632-5p, hsa-miR-3197, hsa-mi R-6798-5p、hsa-miR-711、hsa-miR-6840-3p、hs a-miR-6763-5p, hsa-miR-6727-5p, hsa-miR-37 1a-5p、hsa-miR-6824-5p、hsa-miR-4648、hsa-m iR-1227-5p, hsa-miR-564, hsa-miR-3679-3p, h sa-miR-2861, hsa-miR-6737-5p, hsa-miR-4725 -3p、hsa-miR-6716-5p、hsa-miR-4675、hsa-miR -1915-3p、hsa-miR-671-5p、hsa-miR-3656、hsa -miR-6722-3p、hsa-miR-4707-5p、hsa-miR-444 9、hsa-miR-1202、hsa-miR-4649-5p、hsa-miR-7 44-5p、hsa-miR-642a-3p、hsa-miR-451a、hsa-m iR-6870-5p, hsa-miR-4443, hsa-miR-6808-5p hsa-miR-4728-5p, hsa-miR-937-5p, hsa-miR-1 35a-3p、hsa-miR-663b、hsa-miR-1343-5p、hsa- miR-6822-5p, hsa-miR-6803-5p, hsa-miR-6805 -3p、hsa-miR-128-2-5p、hsa-miR-4640-5p、hsa -miR-1469、hsa-miR-92a-2-5p、hsa-miR-3940- 5p、hsa-miR-4281、hsa-miR-1260b、hsa-miR-47 58-5p、hsa-miR-1915-5p、hsa-miR-5001-5p、hs a-miR-4286、hsa-miR-6126、hsa-miR-6789-5p、 hsa-miR-4459、hsa-miR-1268a、hsa-miR-6752- 5p、hsa-miR-6131、hsa-miR-6800-5p、hsa-miR- 4532、hsa-miR-6872-3p、hsa-miR-718、hsa-miR -6769a-5p、hsa-miR-4707-3p、hsa-miR-6765-5 p, hsa-miR-4739, hsa-miR-4525, hsa-miR-4270 hsa-miR-4534 hsa-miR-6785-5p hsa-miR-68 50-5p、hsa-miR-4697-5p、hsa-miR-1260a、hsa- miR-4486, hsa-miR-6880-5p, hsa-miR-6802-5p hsa-miR-6861-5p hsa-miR-92b-5p hsa-miR- 1238-5p、hsa-miR-6851-5p、hsa-miR-7704、hsa -miR-149-3p、hsa-miR-4689、hsa-miR-4688、hs a-miR-125a-3p、hsa-miR-23b-3p、hsa-miR-614 hsa-miR-1913 hsa-miR-16-5p hsa-miR-6717 -5p, hsa-miR-3648, hsa-miR-3162-5p, hsa-miR -1909-3p、hsa-miR-8073、hsa-miR-6769b-5p、h sa-miR-6836-3p, hsa-miR-4484, hsa-miR-6819 -5p、hsa-miR-6794-5phsa-miR-575、hsa-miR-2 4-3p, hsa-miR-675-5p, hsa-miR-486-3p, hsa-m iR-6777-5p, hsa-miR-4497, hsa-miR-296-3p, h sa-miR-6738-5p, hsa-miR-4731-5p, hsa-miR-6 889-5p, hsa-miR-6786-5p, hsa-miR-92a-3p, hs a-miR-4294, hsa-miR-4763-3p, hsa-miR-6076, hsa-miR-663a, hsa-miR-760, hsa-miR-4667-5p , hsa-miR-6090, hsa-miR-4730, hsa-miR-7106- 5p, hsa-miR-3196, hsa-miR-5698, hsa-miR-608 7, hsa-miR-4665-5p, hsa-miR-8059 and hsa-miR- This includes 6879-5p), its homologues, its transcripts, and its variants or derivatives. Here, genes, congeners, transcripts, mutants, and derivatives are defined as described above.

[0296] Preferred target nucleic acids include human genomes containing a base sequence represented by any of SEQ ID NOs: 1 to 700. A gene, or its transcript, more preferably the transcript, i.e., miRNA, It is a precursor RNA, either pri-miRNA or pre-miRNA.

[0297] The first target genes are the hsa-miR-204-3p gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0298] The second target gene is the hsa-miR-1247-3p gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0299] The third target gene is the hsa-miR-6875-5p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0300] The fourth target gene is the hsa-miR-6857-5p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0301] The fifth target gene is the hsa-miR-6726-5p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0302] The sixth target gene is the hsa-miR-3188 gene, its congeners, and their derivatives. These are the photoproducts, or their variants or derivatives. To date, this gene or its transcripts have been produced. There are no known reports suggesting that changes in the expression of certain substances can serve as markers for esophageal cancer.

[0303] The seventh target gene is the hsa-miR-8069 gene, its congeners, and their derivatives. These are the photoproducts, or their variants or derivatives. To date, this gene or its transcripts have been produced. There are no known reports suggesting that changes in the expression of certain substances can serve as markers for esophageal cancer.

[0304] The eighth target gene is the hsa-miR-4257 gene, its congeners, and their derivatives. These are the photoproducts, or their variants or derivatives. To date, this gene or its transcripts have been produced. There are no known reports suggesting that changes in the expression of certain substances can serve as markers for esophageal cancer.

[0305] The ninth target gene is the hsa-miR-1343-3p gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0306] The tenth target gene is the hsa-miR-7108-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0307] The eleventh target gene is the hsa-miR-6825-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0308] The twelfth target gene is the hsa-miR-7641 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0309] The 13th target gene is the hsa-miR-3185 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0310] The 14th target gene is the hsa-miR-4746-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0311] The 15th target gene is the hsa-miR-6791-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0312] The 16th target gene is the hsa-miR-6893-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0313] The 17th target gene is the hsa-miR-4433b-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0314] The 18th target gene is the hsa-miR-3135b gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0315] The 19th target gene is the hsa-miR-6781-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0316] The 20th target gene is the hsa-miR-1908-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0317] The 21st target genes are the hsa-miR-4792 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0318] The 22nd target gene is the hsa-miR-7845-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0319] The 23rd target gene is the hsa-miR-4417 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0320] The 24th target gene is the hsa-miR-3184-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0321] The 25th target gene is the hsa-miR-1225-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0322] The 26th target gene is the hsa-miR-1231 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0323] The 27th target gene is the hsa-miR-1225-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0324] The 28th target gene is the hsa-miR-150-3p gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0325] The 29th target gene is the hsa-miR-4433-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0326] The 30th target gene is the hsa-miR-6125 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0327] The 31st target genes are the hsa-miR-4513 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0328] The 32nd target gene is the hsa-miR-6787-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0329] The 33rd target gene is the hsa-miR-6784-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0330] The 34th target gene is the hsa-miR-615-5p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0331] The 35th target gene is the hsa-miR-6765-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0332] The 36th target gene is the hsa-miR-5572 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0333] The 37th target gene is the hsa-miR-6842-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0334] The 38th target gene is the hsa-miR-8063 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0335] The 39th target gene is the hsa-miR-6780b-5p gene and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0336] The 40th target gene is the hsa-miR-187-5p gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0337] The 41st target gene is the hsa-miR-128-1-5p gene and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0338] The 42nd target gene is the hsa-miR-6729-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0339] The 43rd target gene is the hsa-miR-6741-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0340] The 44th target gene is the hsa-miR-6757-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0341] The 45th target gene is the hsa-miR-7110-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0342] The 46th target gene is the hsa-miR-7975 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0343] The 47th target gene is the hsa-miR-1233-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0344] The 48th target gene is the hsa-miR-6845-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0345] The 49th target gene is the hsa-miR-3937 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0346] The 50th target gene is the hsa-miR-4467 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0347] The 51st target gene is the hsa-miR-7109-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0348] The 52nd target gene is the hsa-miR-6088 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0349] The 53rd target gene is the hsa-miR-6782-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0350] The 54th target gene is the hsa-miR-5195-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0351] The 55th target gene is the hsa-miR-4454 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0352] The 56th target gene is the hsa-miR-6724-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0353] The 57th target gene is the hsa-miR-8072 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0354] The 58th target gene is the hsa-miR-4516 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0355] The 59th target gene is the hsa-miR-6756-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0356] The 60th target gene is the hsa-miR-4665-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0357] The 61st target gene is the hsa-miR-6826-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0358] The 62nd target gene is the hsa-miR-6820-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0359] The 63rd target gene is the hsa-miR-6887-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0360] The 64th target gene is the hsa-miR-3679-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0361] The 65th target gene is the hsa-miR-7847-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0362] The 66th target gene is the hsa-miR-6721-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0363] The 67th target gene is the hsa-miR-3622a-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0364] The 68th target gene is the hsa-miR-939-5p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0365] The 69th target gene is the hsa-miR-602 gene, its congeners, and their derivatives. These are the photoproducts, or their variants or derivatives. To date, this gene or its transcripts have been produced. There are no known reports suggesting that changes in the expression of certain substances can serve as markers for esophageal cancer.

[0366] The 70th target gene is the hsa-miR-7977 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0367] The 71st target gene is the hsa-miR-6749-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0368] The 72nd target gene is the hsa-miR-1914-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0369] The 73rd target gene is the hsa-miR-4651 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0370] The 74th target gene is the hsa-miR-4695-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0371] The 75th target gene is the hsa-miR-6848-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0372] The 76th target gene is the hsa-miR-1228-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0373] The 77th target gene is the hsa-miR-642b-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0374] The 78th target gene is the hsa-miR-6746-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0375] The 79th target gene is the hsa-miR-3620-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0376] The 80th target gene is the hsa-miR-3131 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0377] The 81st target gene is the hsa-miR-6732-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0378] The 82nd target gene is the hsa-miR-7113-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0379] The 83rd target gene is the hsa-miR-23a-3p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0380] The 84th target gene is the hsa-miR-3154 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0381] The 85th target gene is the hsa-miR-4723-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0382] The 86th target gene is the hsa-miR-3663-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0383] The 87th target gene is the hsa-miR-4734 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0384] The 88th target gene is the hsa-miR-6816-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0385] The 89th target gene is the hsa-miR-4442 gene, its congeners, and their These are transcripts, or their variants or derivatives. To date, genes or their transcripts have been produced. There are no known reports suggesting that changes in the expression of certain substances can serve as markers for esophageal cancer.

[0386] The 90th target gene is the hsa-miR-4476 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0387] The 91st target gene is the hsa-miR-423-5p gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0388] The 92nd target gene is the hsa-miR-1249 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0389] The 93rd target gene is the hsa-miR-6515-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0390] The 94th target gene is the hsa-miR-887-3p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0391] The 95th target gene is the hsa-miR-4741 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0392] The 96th target gene is the hsa-miR-6766-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0393] The 97th target gene is the hsa-miR-4673 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0394] The 98th target gene is the hsa-miR-6779-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0395] The 99th target gene is the hsa-miR-4706 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0396] The 100th target gene is the hsa-miR-1268b gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0397] The 101st target gene is the hsa-miR-4632-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0398] The 102nd target gene is the hsa-miR-3197 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0399] The 103rd target gene is the hsa-miR-6798-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0400] The 104th target gene is the hsa-miR-711 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0401] The 105th target gene is the hsa-miR-6840-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0402] The 106th target gene is the hsa-miR-6763-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0403] The 107th target gene is the hsa-miR-6727-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0404] The 108th target gene is the hsa-miR-371a-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0405] The 109th target gene is the hsa-miR-6824-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0406] The 110th target gene is the hsa-miR-4648 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0407] The 111th target gene is the hsa-miR-1227-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0408] The 112th target gene is the hsa-miR-564 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0409] The 113th target gene is the hsa-miR-3679-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0410] The 114th target gene is the hsa-miR-2861 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0411] The 115th target gene is the hsa-miR-6737-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0412] The 116th target gene is the hsa-miR-575 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. It is known that changes in the expression of certain products can serve as markers for esophageal cancer (Patent Document). 1).

[0413] The 117th target gene is the hsa-miR-4725-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0414] The 118th target gene is the hsa-miR-6716-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0415] The 119th target gene is the hsa-miR-4675 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0416] The 120th target gene is the hsa-miR-1915-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0417] The 121st target gene is the hsa-miR-671-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0418] The 122nd target gene is the hsa-miR-3656 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0419] The 123rd target gene is the hsa-miR-6722-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0420] The 124th target gene is the hsa-miR-4707-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0421] The 125th target gene is the hsa-miR-4449 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0422] The 126th target gene is the hsa-miR-1202 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0423] The 127th target gene is the hsa-miR-4649-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0424] The 128th target gene is the hsa-miR-744-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0425] The 129th target gene is the hsa-miR-642a-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0426] The 130th target gene is the hsa-miR-451a gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0427] The 131st target gene is the hsa-miR-6870-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0428] The 132nd target gene is the hsa-miR-4443 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0429] The 133rd target gene is the hsa-miR-6808-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0430] The 134th target gene is the hsa-miR-4728-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0431] The 135th target gene is the hsa-miR-937-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0432] The 136th target gene is the hsa-miR-135a-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0433] The 137th target gene is the hsa-miR-663b gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0434] The 138th target gene is the hsa-miR-1343-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0435] The 139th target gene is the hsa-miR-6822-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0436] The 140th target gene is the hsa-miR-6803-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0437] The 141st target gene is the hsa-miR-6805-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0438] The 142nd target gene is the hsa-miR-128-2-5p gene and its congeners. , their transcripts, or their variants or derivatives. To date, this gene or There are no known reports that changes in the expression of that transcript can serve as a marker for esophageal cancer. stomach.

[0439] The 143rd target gene is the hsa-miR-4640-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0440] The 144th target gene is the hsa-miR-1469 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0441] The 145th target gene is the hsa-miR-92a-2-5p gene and its congeners. , their transcripts, or their variants or derivatives. To date, this gene or There are no known reports that changes in the expression of that transcript can serve as a marker for esophageal cancer. stomach.

[0442] The 146th target gene is the hsa-miR-3940-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0443] The 147th target gene is the hsa-miR-4281 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0444] The 148th target gene is the hsa-miR-1260b gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0445] The 149th target gene is the hsa-miR-4758-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0446] The 150th target gene is the hsa-miR-1915-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0447] The 151st target gene is the hsa-miR-5001-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0448] The 152nd target gene is the hsa-miR-4286 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0449] The 153rd target gene is the hsa-miR-6126 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0450] The 154th target gene is the hsa-miR-6789-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0451] The 155th target gene is the hsa-miR-4459 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0452] The 156th target gene is the hsa-miR-1268a gene, its congener, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0453] The 157th target gene is the hsa-miR-6752-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0454] The 158th target gene is the hsa-miR-6131 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0455] The 159th target gene is the hsa-miR-6800-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0456] The 160th target gene is the hsa-miR-4532 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0457] The 161st target gene is the hsa-miR-6872-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0458] The 162nd target gene is the hsa-miR-718 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0459] The 163rd target gene is the hsa-miR-6769a-5p gene and its congeners. , their transcripts, or their variants or derivatives. To date, this gene or There are no known reports that changes in the expression of that transcript can serve as a marker for esophageal cancer. stomach.

[0460] The 164th target gene is the hsa-miR-4707-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0461] The 165th target gene is the hsa-miR-6765-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0462] The 166th target gene is the hsa-miR-4739 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0463] The 167th target gene is the hsa-miR-4525 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0464] The 168th target genes are the hsa-miR-4270 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0465] The 169th target gene is the hsa-miR-4534 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0466] The 170th target gene is the hsa-miR-6785-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0467] The 171st target gene is the hsa-miR-6850-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0468] The 172nd target gene is the hsa-miR-4697-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0469] The 173rd target gene is the hsa-miR-1260a gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0470] The 174th target gene is the hsa-miR-4486 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0471] The 175th target gene is the hsa-miR-6880-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0472] The 176th target gene is the hsa-miR-6802-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0473] The 177th target gene is the hsa-miR-6861-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0474] The 178th target gene is the hsa-miR-92b-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0475] The 179th target gene is the hsa-miR-1238-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0476] The 180th target gene is the hsa-miR-6851-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0477] The 181st target genes are the hsa-miR-7704 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0478] The 182nd target gene is the hsa-miR-149-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0479] The 183rd target gene is the hsa-miR-4689 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0480] The 184th target gene is the hsa-miR-4688 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0481] The 185th target gene is the hsa-miR-125a-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0482] The 186th target gene is the hsa-miR-23b-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0483] The 187th target gene is the hsa-miR-614 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of this product can serve as a marker for esophageal cancer.

[0484] The 188th target gene is the hsa-miR-1913 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0485] The 189th target gene is the hsa-miR-16-5p gene, its congeners, and These are transcripts of the gene or its variants or derivatives. There are no known reports suggesting that changes in transcript expression can serve as markers for esophageal cancer.

[0486] The 190th target gene is the hsa-miR-675-5p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0487] The 191st target gene is the hsa-miR-486-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0488] The 192nd target gene is the hsa-miR-6777-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0489] The 193rd target gene is the hsa-miR-4497 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0490] The 194th target gene is the hsa-miR-296-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0491] The 195th target gene is the hsa-miR-6738-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0492] The 196th target gene is the hsa-miR-4731-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0493] The 197th target gene is the hsa-miR-6889-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0494] The 198th target gene is the hsa-miR-6786-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0495] The 199th target gene is the hsa-miR-92a-3p gene, its congeners, and These are their transcripts, or their variants or derivatives. To date, this gene or its It has been reported that changes in the expression of the transcript of can serve as a marker for esophageal cancer. Permitted document 1).

[0496] The 200th target gene is the hsa-miR-4294 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0497] The 201st target gene is the hsa-miR-4763-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0498] The 202nd target gene is the hsa-miR-6076 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0499] The 203rd target gene is the hsa-miR-663a gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. It has been reported that changes in the expression of photoproteins can serve as markers for esophageal cancer (Patent document) 1).

[0500] The 204th target gene is the hsa-miR-760 gene, its congeners, and their This refers to the transcript, or its variants or derivatives. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0501] The 205th target gene is the hsa-miR-4667-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0502] The 206th target genes are the hsa-miR-6090 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0503] The 207th target gene is the hsa-miR-4730 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0504] The 208th target gene is the hsa-miR-7106-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0505] The 209th target gene is the hsa-miR-3196 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0506] The 210th target gene is the hsa-miR-5698 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0507] The 211th target gene is the hsa-miR-6087 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0508] The 212th target gene is the hsa-miR-4665-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0509] The 213th target gene is the hsa-miR-8059 gene, its congeners, and their These are transcripts of this gene, or their variants or derivatives. There are no known reports suggesting that changes in the expression of photoproteins can serve as markers for esophageal cancer.

[0510] The 214th target gene is the hsa-miR-6879-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, this gene or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0511] The 215th target gene is the hsa-miR-6717-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, the gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0512] The 216th target gene is the hsa-miR-3648 gene, its congeners, and their It is a transcript of the gene or its transcription. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0513] The 217th target gene is the hsa-miR-3162-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, the gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0514] The 218th target gene is the hsa-miR-1909-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, the gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0515] The 219th target gene is the hsa-miR-8073 gene, its congeners, and their It is a transcript of the gene or its transcription. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0516] The 220th target gene is the hsa-miR-6769b-5p gene and its congeners. , their transcripts, or their variants or derivatives. To date, genes or There are no known reports suggesting that changes in the expression of that transcript could serve as a marker for esophageal cancer. .

[0517] The 221st target gene is the hsa-miR-6836-3p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, the gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0518] The 222nd target gene is the hsa-miR-4484 gene, its congeners, and their It is a transcript of the gene or its transcription. There are no known reports suggesting that changes in the expression of certain products can serve as markers for esophageal cancer.

[0519] The 223rd target gene is the hsa-miR-6819-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, the gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0520] The 224th target gene is the hsa-miR-6794-5p gene, and its congeners. These are their transcripts, or their variants or derivatives. To date, the gene or its There are no known reports suggesting that changes in the expression of this transcript can serve as a marker for esophageal cancer.

[0521] The 225th target gene is the hsa-miR-24-3p gene, its congener, and These are the transcripts of the gene or its variants or derivatives. It has been reported that changes in the expression of photoproteins can serve as markers for esophageal cancer (Patent document) 1). 2. Nucleic acid probes or primers for the detection of esophageal cancer In the present invention, a nucleus capable of specifically binding to the target nucleic acid as an esophageal cancer marker is used. The acid is used as a nucleic acid, such as a nucleic acid probe or primer, for detecting or diagnosing esophageal cancer. It can be used.

[0522] In the present invention, used for detecting or diagnosing esophageal cancer. Possible nucleic acid probes or primers are target nucleic acids as esophageal cancer markers, for example , human-derived hsa-miR-204-3p, hsa-miR-1247-3p, hsa -miR-6875-5p, hsa-miR-6857-5p, hsa-miR-672 6-5p, hsa-miR-3188, hsa-miR-8069, hsa-miR-4 257, hsa-miR-1343-3p, hsa-miR-7108-5p, hsa- miR-6825-5p, hsa-miR-7641, hsa-miR-3185, hs a-miR-4746-3p, hsa-miR-6791-5p, hsa-miR-68 93-5p、hsa-miR-4433b-3p、hsa-miR-3135b、hsa -miR-6781-5p、hsa-miR-1908-5p、hsa-miR-479 2、hsa-miR-7845-5p、hsa-miR-4417、hsa-miR-3 184-5p、hsa-miR-1225-5p、hsa-miR-1231、hsa- miR-1225-3p, hsa-miR-150-3p, hsa-miR-4433- 3p、hsa-miR-6125、hsa-miR-4513、hsa-miR-678 7-5p、hsa-miR-6784-5p、hsa-miR-615-5p、hsa- miR-6765-3p, hsa-miR-5572, hsa-miR-6842-5p hsa-miR-8063 hsa-miR-6780b-5p hsa-miR-1 87-5p、hsa-miR-128-1-5p、hsa-miR-6729-5p、h sa-miR-6741-5p, hsa-miR-6757-5p, hsa-miR-7 110-5p、hsa-miR-7975、hsa-miR-1233-5p、hsa- miR-6845-5p, hsa-miR-3937, hsa-miR-4467, hs a-miR-7109-5p, hsa-miR-6088, hsa-miR-6782- 5p、hsa-miR-5195-3p、hsa-miR-4454、hsa-miR- 6724-5p, hsa-miR-8072, hsa-miR-4516, hsa-mi R-6756-5p, hsa-miR-4665-3p, hsa-miR-6826-5 p、hsa-miR-6820-5p、hsa-miR-6887-5p、hsa-mi R-3679-5p, hsa-miR-7847-3p, hsa-miR-6721-5 p、hsa-miR-3622a-5p、hsa-miR-939-5p、hsa-mi R-602、hsa-miR-7977、hsa-miR-6749-5p、hsa-m iR-1914-3p、hsa-miR-4651、hsa-miR-4695-5p、 hsa-miR-6848-5p、hsa-miR-1228-3p、hsa-miR- 642b-3p、hsa-miR-6746-5p、hsa-miR-3620-5p、 hsa-miR-3131, hsa-miR-6732-5p, hsa-miR-711 3-3p、hsa-miR-23a-3p、hsa-miR-3154、hsa-miR -4723-5p、hsa-miR-3663-3p、hsa-miR-4734、hs a-miR-6816-5p, hsa-miR-4442, hsa-miR-4476 hsa-miR-423-5p, hsa-miR-1249, hsa-miR-6515 -3p、hsa-miR-887-3p、hsa-miR-4741、hsa-miR- 6766-3p、hsa-miR-4673、hsa-miR-6779-5p、hsa -miR-4706、hsa-miR-1268b、hsa-miR-4632-5p、 hsa-miR-3197, hsa-miR-6798-5p, hsa-miR-711 hsa-miR-6840-3p hsa-miR-6763-5p hsa-miR -6727-5p、hsa-miR-371a-5p、hsa-miR-6824-5p hsa-miR-4648 hsa-miR-1227-5p hsa-miR-56 4、hsa-miR-3679-3p、hsa-miR-2861、hsa-miR-6 737-5p、hsa-miR-4725-3p、hsa-miR-6716-5p、h sa-miR-4675、hsa-miR-1915-3p、hsa-miR-671- 5p、hsa-miR-3656、hsa-miR-6722-3p、hsa-miR- 4707-5p, hsa-miR-4449, hsa-miR-1202, hsa-mi R-4649-5p、hsa-miR-744-5p、hsa-miR-642a-3p hsa-miR-451a hsa-miR-6870-5p hsa-miR-44 43、hsa-miR-6808-5p、hsa-miR-4728-5p、hsa-m iR-937-5p, hsa-miR-135a-3p, hsa-miR-663b, h sa-miR-1343-5p, hsa-miR-6822-5p, hsa-miR-6 803-5p、hsa-miR-6805-3p、hsa-miR-128-2-5p、 hsa-miR-4640-5p, hsa-miR-1469, hsa-miR-92a -2-5p、hsa-miR-3940-5p、hsa-miR-4281、hsa-m iR-1260b, hsa-miR-4758-5p, hsa-miR-1915-5p hsa-miR-5001-5p hsa-miR-4286 hsa-miR-61 26、hsa-miR-6789-5p、hsa-miR-4459、hsa-miR- 1268a、hsa-miR-6752-5p、hsa-miR-6131、hsa-m iR-6800-5p, hsa-miR-4532, hsa-miR-6872-3p, hsa-miR-718, hsa-miR-6769a-5p, hsa-miR-470 7-3p, hsa-miR-6765-5p, hsa-miR-4739, hsa-mi R-4525, hsa-miR-4270, hsa-miR-4534, hsa-miR -6785-5p, hsa-miR-6850-5p, hsa-miR-4697-5p , hsa-miR-1260a, hsa-miR-4486, hsa-miR-6880 -5p, hsa-miR-6802-5p, hsa-miR-6861-5p, hsa- miR-92b-5p, hsa-miR-1238-5p, hsa-miR-6851- 5p, hsa-miR-7704, hsa-miR-149-3p, hsa-miR-4 689, hsa-miR-4688, hsa-miR-125a-3p, hsa-miR -23b-3p, hsa-miR-614, hsa-miR-1913, hsa-miR -16-5p, hsa-miR-6717-5p, hsa-miR-3648, hsa- miR-3162-5p, hsa-miR-1909-3p, hsa-miR-8073 , hsa-miR-6769b-5p, hsa-miR-6836-3p, hsa-mi R-4484, hsa-miR-6819-5p, or hsa-miR-6794- 5p or combinations thereof, or their homologues, their transcripts, or their variants. Alternatively, derivatives thereof, and, depending on the circumstances, hsa-mi R-575 or hsa-miR-24-3p, or a combination thereof, or those Congeners of the same, their transcripts, their variants or derivatives, and, in cases thereof More combinations are possible, such as hsa-miR-675-5p and hsa-miR-48. 6-3p, hsa-miR-6777-5p, hsa-miR-4497, hsa-mi R-296-3p, hsa-miR-6738-5p, hsa-miR-4731-5p , hsa-miR-6889-5p, hsa-miR-6786-5p, hsa-miR -92a-3p, hsa-miR-4294, hsa-miR-4763-3p, hsa -miR-6076, hsa-miR-663a, hsa-miR-760, hsa-m iR-4667-5p, hsa-miR-6090, hsa-miR-4730, hsa -miR-7106-5p, hsa-miR-3196, hsa-miR-5698, h sa-miR-6087, hsa-miR-4665-5p, hsa-miR-8059 Alternatively, hsa-miR-6879-5p, or a combination thereof, or related species thereof. Determining the presence, expression level, or abundance of the organism, its transcripts, their variants, or derivatives. To enable sexual and / or quantitative measurement.

[0523] The above target nucleic acids were found to be more effective in subjects with esophageal cancer compared to healthy individuals. Depending on the type, the expression levels of these may increase or decrease (see below, (This is referred to as "increase / decrease.") Therefore, the nucleic acid of the present invention is used in patients suspected of having esophageal cancer. The expression levels of the target nucleic acids mentioned above were measured in bodily fluids derived from test subjects (e.g., humans) and bodily fluids derived from healthy individuals. By comparing them, these can be effectively used to detect esophageal cancer.

[0524] Nucleic acid probes or primers usable in this invention are SEQ ID NOs: 1-115, 117-1 Polynucleo consisting of a base sequence represented by 89 and at least one of 666-675 Nucleic acid probes that can specifically bind to tide, or SEQ ID NOs: 1-115, 117-18 9 and a polynucleotide consisting of a base sequence represented by at least one of 666-675 This is a primer for amplifying the D.

[0525] The nucleic acid probes or primers usable in this invention are further SEQ ID NOs: 116 and 676 A nucleic acid probe that can specifically bind to a polynucleotide consisting of a base sequence represented by [the given formula], i is a p for amplifying polynucleotides consisting of the base sequence represented by sequence number 116. It can include a rhymer.

[0526] The nucleic acid probes or primers usable in this invention are further related to SEQ ID NOs: 190-214. Nucleic acids that can specifically bind to polynucleotides consisting of a base sequence represented by at least one base. From a probe, or from a nucleotide sequence represented by at least one of sequence numbers 190-214 The material may further include primers for amplifying the polynucleotides.

[0527] Specifically, the nucleic acid probes or primers mentioned above are any of the SEQ ID NOs: 1 to 700. Polynes containing the represented base sequence, or the base sequence in which u is t. Cleotide group and its complementary polynucleotide group, from a base sequence complementary to the said base sequence Polynucleotides that hybridize with DNA under stringent conditions (described later) Otide group and its complementary polynucleotide group, and the bases of those polynucleotide groups A group of polynucleotides containing 15 or more, preferably 17 or more, consecutive bases in the sequence. It contains one or more selected combinations of polynucleotides. The do is a nucleic acid probe and primer for detecting the target nucleic acid, which is the esophageal cancer marker. It can be used as -.

[0528] More specifically, examples of nucleic acid probes or primers usable in the present invention are as follows: One or more polynucleotides selected from the group consisting of (a) to (e) That is the case. (a) Represented by one of the sequence numbers 1-115, 117-189, and 666-675 Polynucleotides consisting of a base sequence, or a base sequence in which u is t. Otide, its variants, and its derivatives are fragments containing 15 or more consecutive bases. (b) Represented by any of the sequence numbers 1-115, 117-189, and 666-675 Polynucleotides containing a base sequence (c) Represented by any of the sequence numbers 1-115, 117-189, and 666-675 A base sequence, or a base sequence in which u is t, and a base sequence complementary to that base sequence. A polynucleotide consisting of, its variants, its derivatives, or containing 15 or more consecutive bases Fragments of Muso (d) Represented by one of the sequence numbers 1-115, 117-189, and 666-675 A base sequence, or a base sequence in which u is t, and a base sequence complementary to that base sequence. Polynucleotides containing, (e) With any of the polynucleotides from (a) to (d) above under stringent conditions A polynucleotide that hybridizes.

[0529] The nucleic acid probes or primers usable in the present invention may further include the above polynucleotides ( In addition to at least one polynucleotide selected from the group consisting of a) to (e), the following It may contain polynucleotides selected from the group consisting of (f) to (j). (f) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide consisting of a base sequence t, its variants, its derivatives, or 15 or more The fragment containing consecutive bases, (g) Polynucleotides containing the base sequences represented by SEQ ID NOs. 116 and 676, (h) The base sequence represented by sequence numbers 116 and 676, or in the said base sequence, u A polynucleotide consisting of a base sequence where is t, a base sequence complementary to it, its variant, and Derivatives, or fragments thereof containing 15 or more consecutive bases, (i) The base sequence represented by sequence numbers 116 and 676, or the base sequence in which u A polynucleotide containing a base sequence that is t, and a base sequence that is complementary to it, (j) With any of the polynucleotides in (f) to (i) above under stringent conditions A polynucleotide that hybridizes.

[0530] The nucleic acid probes or primers usable in the present invention may further include the above polynucleotides ( In addition to at least one polynucleotide selected from the group consisting of a) to (j), the following It may contain polynucleotides selected from the group consisting of (k) to (o). (k) A base sequence represented by any of sequence numbers 190 to 214 or the said base sequence A polynucleotide consisting of a base sequence in which u is t, its variants, its derivatives, or 1 The fragment containing 5 or more consecutive bases, (l) Polynucleotides containing a base sequence represented by any of sequence numbers 190-214, (m) A base sequence represented by any of sequence numbers 190-214, or a base sequence containing A polynucleotide consisting of a base sequence where u is t, and a base sequence complementary to it, and its mutation The body, its derivatives, or a fragment thereof containing 15 or more consecutive bases, (n) A base sequence represented by any of sequence numbers 190 to 214, or a base sequence containing A polynucleotide containing a base sequence complementary to the base sequence in which u is t, and (o) With any of the polynucleotides (k) to (n) above under stringent conditions A polynucleotide that hybridizes.

[0531] In the above polynucleotides, "a fragment containing 15 or more consecutive bases" is defined as each polynucleotide. In the base sequence of a nucleotide, for example, from 15 consecutive bases to less than the total number of bases in the sequence, 1 This includes base counts in the range of 7 to less than the total number of bases in the sequence, 19 to less than the total number of bases in the sequence, and so on. These are possible, but are not limited to them.

[0532] The polynucleotides or fragments thereof used in the present invention may also be DNA. RNA is also acceptable.

[0533] The polynucleotides usable in this invention are DNA recombination technology, PCR method, DNA It can be prepared using common techniques such as methods using an automated RNA synthesizer.

[0534] DNA recombination technology and PCR methods are described, for example, by Ausubel et al., Current Pro. tocols in Molecular Biology,John Willey& Sons, US (1993); Sambrook et al., Molecular Clonin g A Laboratory Manual,Cold Spring Harbor Use the techniques described in Laboratory Press, US (1989), etc. It is possible.

[0535] Human-derived hsa-miR-204 represented by SEQ ID NOs: 1-214 and 666-676 -3p, hsa-miR-1247-3p, hsa-miR-6875-5p, hsa- miR-6857-5p, hsa-miR-6726-5p, hsa-miR-3188 , hsa-miR-8069, hsa-miR-4257, hsa-miR-1343- 3p, hsa-miR-7108-5p, hsa-miR-6825-5p, hsa-m iR-7641, hsa-miR-3185, hsa-miR-4746-3p, hsa -miR-6791-5p, hsa-miR-6893-5p, hsa-miR-443 3b-3p, hsa-miR-3135b, hsa-miR-6781-5p, hsa- miR-1908-5p, hsa-miR-4792, hsa-miR-7845-5p , hsa-miR-4417, hsa-miR-3184-5p, hsa-miR-12 25...

Claims

1. A kit for detecting esophageal cancer, comprising a nucleic acid probe that can specifically bind to the miR-6784-5p polynucleotide, which is an esophageal cancer marker and consists of the base sequence represented by Sequence ID No. 33, and / or a primer that specifically recognizes and amplifies said polynucleotide.

2. The kit is another esophageal cancer marker, consisting of the base sequences represented by SEQ ID NOs: 2 to 3, 5 to 7, 9 to 32, 35 to 214, and 666 to 676, namely miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5p, miR-4442miR-4476、miR-423-5p、miR-1249、miR-6515-3p、miR-887-3p、miR-4741、m iR-6766-3p、miR-4673、miR-6779-5p、miR-4706、miR-1268b、miR-4632-5p 、miR-31197、miR-6798-5p、miR-7111、miR-6840-3p、miR-6763-5p、miR-6727 -5p、miR-371a-5p、miR-6824-5p、miR-4648、miR-1227-5p、miR-564、miR-36 79-3p、miR-2861、miR-6737-5p、miR-4725-3p、miR-6716-5p、miR-4675、mi R-119115-3p、miR-671-5p、miR-3656、miR-6722-3p、miR-4707-5p、miR-4449 、miR-11202、miR-4649-5p、miR-744-5p、miR-642a-3p、miR-451a、miR-6870 -5p、miR-4443、miR-6808-5p、miR-4728-5p、miR-937-5p、miR-135a-3p、miR -663b、miR-11343-5p、miR-6822-5p、miR-6803-5p、miR-6805-3p、miR-1128- 2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、miR-3940-5p、miR-4281、mi R-12260b、miR-4758-5p、miR-11915-5p、miR-5001-5p、miR-4286、miR-61126、 miR-6789-5p、miR-4459、miR-1268a、miR-6752-5p、miR-6131、miR-6800-5p 、miR-45332、miR-6872-3p、miR-718、miR-6769a-5p、miR-4707-3p、miR-676 5-5p、miR-4739、miR-4525、miR-4270、miR-4534、miR-6785-5p、miR-6850- 5p、miR-4697-5p、miR-1260a、miR-4486、miR-6880-5p、miR-6802-5p、miR- 88115-5p、miR-92b-5p、miR-1238-5p、miR-68511-5p、miR-7704、miR-149-3p、miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p, m iR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p, miR -6836-3p, miR-4484, miR-6819-5p and miR-6794-5p, miR-575, miR-24-3p, miR-675- 5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, The kit according to claim 1, further comprising a nucleic acid probe capable of specifically binding to at least one polynucleotide selected from the group consisting of miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, and / or a primer that specifically recognizes and amplifies said polynucleotide.

3. A device for detecting esophageal cancer, comprising a nucleic acid probe that is specifically capable of binding to the polynucleotide miR-6784-5p, which is an esophageal cancer marker and consists of the base sequence represented by Sequence ID No. 33, and / or a primer that specifically recognizes and amplifies the polynucleotide.

4. The device is another esophageal cancer marker, and consists of base sequences represented by SEQ ID NO: 2 to 3, 5 to 7, 9 to 32, 35 to 214, and 666 to 676, namely miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5p,miR-4442、miR-4476、miR-423-5p、miR-1249、miR-6515-3p、miR-887-3p、m iR-4741、miR-6766-3p、miR-4673、miR-6779-5p、miR-4706、miR-1268b、mi R-4632-5p、miR-3197、miR-6798-5p、miR-711、miR-6840-3p、miR-6763-5p 、miR-6727-5p、miR-371a-5p、miR-6824-5p、miR-4648、miR-1227-5p、miR- 564、miR-3679-3p、miR-2861、miR-6737-5p、miR-4725-3p、miR-6716-5p、m iR-4675、miR-1915-3p、miR-671-5p、miR-3656、miR-6722-3p、miR-4707-5 p、miR-4449、miR-1202、miR-4649-5p、miR-744-5p、miR-642a-3p、miR-451 a、miR-6870-5p、miR-4443、miR-6808-5p、miR-4728-5p、miR-937-5p、miR-1 35a-3p、miR-663b、miR-1343-5p、miR-6822-5p、miR-6803-5p、miR-6805-3 p、miR-1128-2-5p、miR-4640-5p、miR-11469、miR-92a-2-5p、miR-3940-5p、m iR-4281、miR-1260b、miR-4758-5p、miR-1915-5p、miR-5001-5p、miR-4286 、miR-61226、miR-6789-5p、miR-4459、miR-1268a、miR-6752-5p、miR-61331、 miR-6800-5p、miR-4532、miR-6872-3p、miR-718、miR-6769a-5p、miR-4707 -3p、miR-6765-5p、miR-4739、miR-4525、miR-4270、miR-4534、miR-6785-5 p、miR-6850-5p、miR-4697-5p、miR-1260a、miR-4486、miR-6880-5p、miR-6 802-5p、miR-6861-5p、miR-92b-5p、miR-1238-5p、miR-6851-5p、miR-7704、miR-149-3p, miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, m iR-16-5p, miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769 b-5p, miR-6836-3p, miR-4484, miR-6819-5p, miR-6794-5p, miR-575, miR-24-3p, miR -675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731 The device according to claim 3, further comprising a nucleic acid probe capable of specifically binding to at least one polynucleotide selected from the group consisting of -5p, miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, and / or a primer that specifically recognizes and amplifies said polynucleotide.

5. The device according to claim 3 or 4, wherein the device is a device for measurement by hybridization technology.

6. The device according to claim 5, wherein the hybridization technology is nucleic acid array technology.

7. A method for assisting the detection of esophageal cancer, comprising measuring the expression level of miR-6784-5p in a subject's sample using the kit described in claim 1 or the device described in claim 3 or claim 5 or 6 dependent on claim 3, comparing the measured expression level of the subject with the similarly measured control expression level of a healthy person, and evaluating in vitro whether the subject has esophageal cancer when the expression level of the subject is higher than that of a healthy person.

8. The method according to claim 7, wherein the subject is a human being.

9. The method according to claim 7 or 8, wherein the specimen is blood, serum, or plasma.

10. The use of miR-6784-5p, which consists of the nucleotide sequence represented by Sequence ID No. 33, as an esophageal cancer marker for the detection of esophageal cancer, The expression level of miR-6784-5p in the subject's sample is measured, and the measured expression level is compared with the control expression level of a healthy individual measured in the same way. If the expression level in the subject is higher than the control expression level, the subject is evaluated as having esophageal cancer. The aforementioned use.

11. The miR-6784-5p consists of the base sequences represented by SEQ ID NOs: 2 to 3, 5 to 7, 9 to 32, 35 to 214, and 666 to 676, respectively, and is miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5p, miR-4442miR-4476、miR-423-5p、miR-1249、miR-6515-3p、miR-887-3p、miR-4741、m iR-6766-3p、miR-4673、miR-6779-5p、miR-4706、miR-1268b、miR-4632-5p 、miR-31197、miR-6798-5p、miR-7111、miR-6840-3p、miR-6763-5p、miR-6727 -5p、miR-371a-5p、miR-6824-5p、miR-4648、miR-1227-5p、miR-564、miR-36 79-3p、miR-2861、miR-6737-5p、miR-4725-3p、miR-6716-5p、miR-4675、mi R-119115-3p、miR-671-5p、miR-3656、miR-6722-3p、miR-4707-5p、miR-4449 、miR-11202、miR-4649-5p、miR-744-5p、miR-642a-3p、miR-451a、miR-6870 -5p、miR-4443、miR-6808-5p、miR-4728-5p、miR-937-5p、miR-135a-3p、miR -663b、miR-11343-5p、miR-6822-5p、miR-6803-5p、miR-6805-3p、miR-1128- 2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、miR-3940-5p、miR-4281、mi R-12260b、miR-4758-5p、miR-11915-5p、miR-5001-5p、miR-4286、miR-61126、 miR-6789-5p、miR-4459、miR-1268a、miR-6752-5p、miR-6131、miR-6800-5p 、miR-45332、miR-6872-3p、miR-718、miR-6769a-5p、miR-4707-3p、miR-676 5-5p、miR-4739、miR-4525、miR-4270、miR-4534、miR-6785-5p、miR-6850- 5p、miR-4697-5p、miR-1260a、miR-4486、miR-6880-5p、miR-6802-5p、miR- 88115-5p、miR-92b-5p、miR-1238-5p、miR-68511-5p、miR-7704、miR-149-3p、miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p , miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p , miR-6836-3p, miR-4484, miR-6819-5p, miR-6794-5p, miR-575, miR-24-3p, miR -675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR- The use according to claim 10, in combination with at least one polynucleotide selected from the group consisting of 4731-5p, miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, used as an esophageal cancer marker for the detection of esophageal cancer.

Images