Dynamic cell culture platform and method thereof

Abstract

The present invention relates to a dynamic cell culture platform. More specifically, the dynamic cell culture platform of the present invention comprises a lower fixing frame for fixing a well plate, an upper fixing frame for fixing a pillar plate, and an actuator having one end fixed to the lower fixing frame and the other end fixed to the upper fixing frame, which repeatedly controls the upper fixing frame to move up and down to repeatedly move the pillar plate in a direction that connects to and separates from the well plate. In this way, the efficiency of organoid culture dispensed at the end of the pillar can be improved by generating a fluid flow of the culture medium contained in the well through the repetitive up-and-down movement of the well-pillar plate.

Description

Technology Field

[0001] The present invention relates to a dynamic cell culture platform, and more specifically, to a dynamic cell culture platform capable of improving the efficiency of organoid culture dispensed at the end of a pillar by generating a fluid flow of culture medium contained in the well through the repetitive up-and-down movement of a well-pillar plate. Background Technology

[0003] Organoid cell culture is a three-dimensional culture technology using stem cells or patient-derived cells that mimics the functions of actual human cells, holding great potential in various fields such as disease modeling, new drug development, regenerative medicine, and precision medicine. Through this technology, it is possible to gain a deep understanding of disease mechanisms using patient-specific disease models, improve the accuracy of evaluating the efficacy and safety of new drugs, and implement personalized treatment strategies in precision medicine.

[0004] Traditional organoid culture methods included suspending cells in a culture medium using agar or anti-adhesion coatings, or growing cell clusters in hydrogels within wells. These methods had the disadvantage that it was difficult to replace the culture medium, drugs, and staining reagents. To overcome this, a hydrogels-on-micropillar culture model with a pillar-well structure was developed.

[0005] In this structure, the biochip consists of a pillar plate and a well plate; the pillar plate integrally forms a protruding pillar portion in a column shape, and the well plate includes a well portion capable of accommodating the pillar portion. Culture is performed by placing the pillar plate on top of the well plate, thereby immersing the cells located in the pillar portion into the culture medium in the well portion. Additionally, the biochip can measure cells using a microscope by utilizing a light source that passes through the pillar portion.

[0006] However, this method may occasionally result in a problem where the culture medium does not sufficiently permeate the cells. Accordingly, the inventors intend to provide a dynamic cell culture platform that can solve the problems of existing cell culture methods and increase the efficiency of cell culture. The problem to be solved

[0008] The problem that the present invention aims to solve is to provide a dynamic cell culture platform and a method thereof that can improve the efficiency of organoid culture dispensed at the end of a pillar by generating fluid flow of the culture medium contained in the well through the repetitive up-and-down movement of the well-pillar plate.

[0010] The problems of the present invention are not limited to those mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the description below. means of solving the problem

[0012] To solve the problem described above, a dynamic cell culture platform according to one embodiment of the present invention may include a lower fixing frame for fixing a well plate, an upper fixing frame for fixing a pillar plate, and an actuator having one end fixed to the lower fixing frame and the other end fixed to the upper fixing frame, which repeatedly controls the upper fixing frame to move up and down to repeatedly move the pillar plate in a direction of coupling to and separation from the well plate.

[0013] A dynamic cell culture method of a dynamic cell culture platform according to one embodiment of the present invention, comprising a lower fixing frame for fixing a well plate, an upper fixing frame for fixing a pillar plate, and an actuator having one end fixed to the lower fixing frame and the other end fixed to the upper fixing frame and controlling the up-and-down movement of the upper fixing frame, may include the steps of: (a) preparing a well plate containing a culture medium and a pillar plate having an organoid dispensed at one end; (b) fixing the well plate to the lower fixing frame; (c) fixing the pillar plate to the upper fixing frame; and (d) culturing the organoid while controlling the attachment and separation of the pillar plate to the well plate by the actuator repeatedly moving the upper fixing frame up and down.

[0015] Specific details of other embodiments are included in the detailed description and drawings. Effects of the invention

[0017] A dynamic cell culture platform and method according to one embodiment of the present invention can improve the efficiency of organoid culture dispensed at the end of a pillar by generating a fluid flow of culture medium contained in the well through the repetitive up-and-down movement of a well-pillar plate.

[0018] Specifically, dynamic culture environments can more accurately mimic the actual environment of in vivo tissues. Furthermore, in dynamic cell culture, continuous fluid flow efficiently supplies nutrients and oxygen to cells and removes metabolic waste.

[0019] In addition, dynamic culture environments can enhance cell growth, differentiation, and functional activity. Furthermore, dynamic culture can reduce necrosis of the center of the 3D structure.

[0020] Furthermore, shear stress caused by fluid flow provides important mechanical stimuli for the differentiation and function of specific cell types, which can contribute to more accurately reflecting the physiological responses of cells.

[0021] Furthermore, cells cultured in a dynamic environment can better mimic the responses of in vivo cells, which can play an important role in improving the accuracy of drug screening. In addition, thanks to the continuous supply of nutrients and the removal of metabolic waste, dynamic 3D cell culture enables long-term cell culture, which can be a significant advantage for chronic disease models or studies on long-term drug responses.

[0022] In addition, this dynamic environment can facilitate the formation of more complex and tissue-like 3D structures. This can play an important role in generating cell models that mimic real tissues.

[0024] The effects according to the present invention are not limited to those exemplified above, and other effects will be clearly understood by those skilled in the art from the description in the specification below. Brief explanation of the drawing

[0026] FIG. 1 is a perspective view showing a dynamic cell culture platform according to one embodiment of the present invention. Figure 2 is a diagram illustrating the structure of the dynamic cell culture platform of Figure 1. Figure 3 is a front view of the actuator of the dynamic cell culture platform of Figure 1 during the first operation (movement in the coupling direction). Fig. 4 is a side view of Fig. 3. Figure 5 is a front view of the actuator of the dynamic cell culture platform of Figure 1 during the second operation (movement in the separation direction). Fig. 6 is a side view of Fig. 5. FIG. 7 is a drawing showing the first operation of FIG. 3 and the second operation of FIG. 5. FIG. 8 is a drawing for explaining the stroke (S) according to the structure of a well-pillar plate according to one embodiment of the present invention. FIG. 9 is a flowchart illustrating a dynamic cell culture method according to one embodiment of the present invention. Specific details for implementing the invention

[0027] The following description merely illustrates the principles of the invention. Therefore, those skilled in the art may invent various devices that embody the principles of the invention and are included within the concept and scope of the invention, even if they are not explicitly described or illustrated in this specification. Furthermore, all conditional terms and embodiments listed in this specification are, in principle, explicitly intended only for the purpose of understanding the concept of the invention and should be understood as not being limited to the embodiments and conditions specifically listed as such.

[0028] Furthermore, in the following description, ordinal expressions such as "first," "second," etc., are intended to describe mutually equal and independent objects, and should be understood as having no meaning of main / sub or master / slave in their order.

[0029] The aforementioned objectives, features, and advantages will become clearer through the following detailed description in conjunction with the attached drawings, and accordingly, a person skilled in the art to which the invention pertains will be able to easily implement the technical concept of the invention.

[0030] The features of each of the various embodiments of the present invention may be combined or combined with one another, either partially or wholly, and as will be fully understood by those skilled in the art, various technical interlocking and operation are possible, and each embodiment may be implemented independently of one another or together in an interlocking relationship.

[0031] Hereinafter, various embodiments of the present invention will be described in detail with reference to the attached drawings.

[0033] The main reasons for utilizing dynamic 3D cell culture in the present invention are as follows.

[0034] First, dynamic culture environments can more accurately mimic the actual environment of body tissues. Since cells in the body experience processes such as continuous fluid flow, nutrient supply, and the removal of metabolic waste, reproducing this environment is important for properly understanding the physiological functions of cells.

[0035] Second, in dynamic cell culture, continuous fluid flow allows for the efficient supply of nutrients and oxygen to cells and the removal of metabolic waste. This is particularly important when culturing large 3D structures, as in static culture environments, the cells in the center may necrose due to nutrient deficiency and waste accumulation.

[0036] Third, dynamic culture environments can enhance cell growth, differentiation, and functional activity. Improved nutrient supply and the removal of metabolic waste optimize the physiological state of cells, which provides a model that more accurately reflects the functional characteristics of the cells.

[0037] Fourth, dynamic culture can reduce necrosis in the center of 3D structures. In static culture, the center of large 3D structures often necroses due to a lack of nutrient and oxygen supply, but in dynamic culture, fluid flow helps solve this problem.

[0038] Fifth, shear stress caused by fluid flow provides important mechanical stimulation for the differentiation and function of specific cell types. This mechanical stimulation contributes to a more accurate reflection of the cell's physiological response.

[0039] Sixth, cells cultured in a dynamic environment can better mimic the responses of in vivo cells, playing a crucial role in improving the accuracy of drug screening. This can increase the efficiency of new drug development by providing more predictive models during the drug development process.

[0040] Seventh, thanks to continuous nutrient supply and removal of metabolic waste, dynamic 3D cell culture enables long-term cell culture. This is a significant advantage for chronic disease models or studies of long-term drug responses.

[0041] Finally, dynamic environments can facilitate the formation of more complex and tissue-like 3D structures. This plays a crucial role in generating cell models that mimic actual tissues. Therefore, dynamic 3D cell culture provides physiologically more relevant and predictive in vitro models, making it a highly useful technique for drug development and disease research.

[0042] In the present invention, dynamic 3D cell culture can be performed according to a dynamic cell culture platform and a dynamic cell culture method using the dynamic cell culture platform. This will be explained in detail with reference to FIGS. 1 to 9.

[0043] FIG. 1 is a perspective view showing a dynamic cell culture platform according to an embodiment of the present invention. FIG. 2 is a drawing for explaining the structure of the dynamic cell culture platform of FIG. 1. FIG. 3 is a front view of the actuator of the dynamic cell culture platform of FIG. 1 during the first operation (movement in the coupling direction). FIG. 4 is a side view of FIG. 3. FIG. 5 is a front view of the actuator of the dynamic cell culture platform of FIG. 1 during the second operation (movement in the separation direction). FIG. 6 is a side view of FIG. 5. FIG. 7 is a drawing showing the first operation of FIG. 3 and the second operation of FIG. 5.

[0044] Referring to FIGS. 1 to 7, a dynamic cell culture platform according to one embodiment of the present invention may include a lower fixing frame (300) for fixing a well plate (100), an upper fixing frame (400) for fixing a pillar plate (200), and an actuator (500), and additionally may further include a moving guide part (600a to 600d).

[0045] The lower fixed frame (300) supports the well plate (100) and can be seated and fixed. The lower fixed frame (300) may include a lower support plate (310) and a seating portion (320).

[0046] The lower support plate (310) may be formed as a flat frame such as a square shape, and the seating portion (320) may include a groove (321) that is spaced apart from the lower support plate (310) at a predetermined interval and supports the well plate (100).

[0047] At this time, the seating portion (320) may be formed to correspond to the size of the well plate (100), and a seating groove (321) may be formed to stably seat and fix the well plate (100) being seated, and the well plate (100) may be seated within the seating groove (321).

[0048] The upper fixed frame (400) can support and fix the pillar plate (200). At this time, the upper fixed frame (400) can be positioned parallel to the lower fixed frame (300) and positioned vertically above at a certain distance from the lower fixed frame (300).

[0049] The upper fixed frame (400) may include an upper support plate (410) and a cover portion (420). The upper support plate (410) may have a through hole (411) through which the pillar (Fig. 7, 220) of the pillar plate (200) passes at a position corresponding to the seating portion (320), and a step (412) on which the edge region of the plate (Fig. 7, 210) of the pillar plate (200) is caught. At this time, the step (412) may be formed as a first step on which the pillar plate (200) is caught and seated, and a second step on which the cover portion (420) is caught and seated, forming a step difference from the first step.

[0050] The cover portion (420) can be stacked on the upper part of the plate (210) of the pillar plate (200) which is engaged with the step (412), and can be secured by being seated and coupled to the step (412) to fix the pillar plate (200). The cover portion (420) may have a fixed coupling structure, such as a snap-fit ​​coupling, with the upper support plate (410).

[0051] One end of the actuator (500) can be fixed to the lower fixed frame (300) and the other end can be fixed to the upper fixed frame (400), and the upper fixed frame (400) can be controlled to move up and down repeatedly a preset number of times during cell culture.

[0052] Specifically, one end of the actuator (500) can be fixedly connected to the center of the upper surface of the lower fixed frame (300) by means of a screw connection, and the other end can be fixedly connected to the center of the lower surface of the upper fixed frame (400) by means of a screw connection, etc., so that it can perform vertical linear motion. At this time, while the lower fixed frame (300) is fixed, only the upper fixed frame (400) can be moved up and down a predetermined number of times at a predetermined stroke speed within a predetermined stroke length (S).

[0053] In addition, the present invention may further include a plurality of moving guide parts (600a to 600d) in the corner area between the lower fixed frame (300) and the upper fixed frame (400).

[0054] A plurality of moving guide sections (600a to 600d) are each placed in a corner area between the lower fixed frame (300) and the upper fixed frame (400), and can guide the vertical movement of the upper fixed frame (400), which moves vertically by an actuator (500) and whose position is variable, so that the vertical movement can be stably controlled. To this end, the plurality of moving guide sections (600a to 600d) may include a guide shaft (610), a guide pipe (620), and a coupling section (630).

[0055] The guide shaft (610) has one end fixed to the upper corner of the lower support plate (310) of the lower fixed frame (300) and one end can form a length by penetrating the lower corner of the upper support plate (410) of the corresponding upper fixed frame (400). That is, the guide shaft (610) can be a fixed shaft fixed to the lower fixed frame (300), and the upper fixed frame (400) can move up and down by the actuator (500) through a structure that penetrates the upper fixed frame (400).

[0056] The guide pipe (620) is in the form of a pipe that forms a hollow space and can be formed with a length shorter than the guide shaft (610), and the guide shaft (610) can be inserted into the hollow space. One end of the guide pipe (620) is fixedly connected to the upper fixed frame (400) so that it can slide along the guide shaft (610) when the actuator is driven.

[0057] At this time, the guide pipe (620) may be fixed to the upper fixed frame (400) in a structure in which the upper side of the guide pipe (620) is fixedly coupled to the corner lower surface of the upper fixed frame (400), or the guide pipe (620) may be fixed through a coupling part (630) that is coupled to the corner upper surface of the upper fixed frame (620) by means of screw coupling, etc.

[0058] In this way, dynamic cell culture can be performed by placing a well-pillar plate on a dynamic cell culture platform according to the structure of FIGS. 1 to 6. At this time, the present invention can control the stroke conditions of an actuator (500) according to the structure of the well-pillar plate. This can be explained through FIGS. 7 and FIGS. 8. FIGS. 8 is a drawing for explaining the stroke (S) according to the structure of a well-pillar plate according to an embodiment of the present invention.

[0059] As shown in FIGS. 7 and 8, the well-pillar plate may be composed of a well plate (100) divided into multiple channels for holding culture medium (10) and multiple pillars (220) formed by extending vertically from the plate (210).

[0060] In the well plate (100), a culture medium (10) can be contained in the well channels, and in the pillar plate (200), organoids (20) can be dispensed at the ends of a plurality of pillars (220) that are vertically extended from the plate (210).

[0061] Figure 7(a) is a state in which, due to the downward movement of the actuator (500) as in Figures 3 and 4, the pillar (220) of the pillar plate (200) fixed to the upper fixed frame (400) moves in a direction that connects to the well plate (100) and is immersed in the culture medium (10).

[0062] Meanwhile, in FIG. 7(b), as in FIG. 5 and FIG. 6, the pillar (220) of the pillar plate (200) fixed to the upper fixed frame (400) can be moved in a direction separated from the well plate (100) due to the upward movement of the actuator (500). At this time, it is moved according to the stroke length (S), and the organoid dispensed at the end of the pillar (220) can be moved until it is immersed in the culture medium. That is, when controlling the vertical movement of the actuator (500), the organoid (20) dispensed at the end of the pillar plate (200) can be dynamically cultured while moving vertically while immersed in the culture medium (10).

[0063] To this end, in the present invention, a stroke (S) based on a well-pillar structure (well width, pillar length, pillar diameter) as shown in FIG. 8 is determined, and a fluid flow can be generated by driving an actuator (500) by adjusting the stroke speed, etc., so that the culture medium does not overflow.

[0064] The stroke length (S) of the actuator (500) during vertical movement can be calculated by the following mathematical formulas 1 and 2.

[0065]

[0066]

[0067] In mathematical formula 1, can be the immersion area media volume when the end of the pillar plate (200) is joined to the well plate (100) at the maximum depth as in FIG. 8 (a). Also, in Equation 1 The immersion area media volume can be when the end of the pillar plate (200) is joined to the well plate (100) at a minimum depth as in Fig. 8 (b).

[0068] In this case, since there is no change in the amount of culture medium, the immersion area media volume can be the same value in both cases. Additionally, W can be the well width, D the filament diameter, and L the filament length.

[0069] Fluid flow can be generated by adjusting the number of strokes and speed based on the stroke length (S), well capacity, and amount of culture medium obtained here.

[0070] For example, when using a 384 plate, the stroke length (S) can be calculated as 13.8 mm as follows. Here, W can be 3.4 mm, D can be 2 mm, and L can be 20 mm.

[0071] (mm)

[0072] In this case, if the actuator (500) is driven at a speed of 0.43 mm / sec, a flow rate of 5 μL / sec can be generated. Through this, the organoid dispensed to the immersed pillar end can be dynamically cultured.

[0073] FIG. 9 is a flowchart illustrating a dynamic cell culture method according to an embodiment of the present invention. A dynamic cell culture method according to an embodiment of the present invention may be performed based on the dynamic cell culture platform of FIGS. 1 to 8. Accordingly, the method will be described with reference to FIGS. 1 to 8. At this time, the processes may be performed by a robot.

[0074] First, a well plate (100) containing a culture medium and a pillar plate (200) with organoids dispensed at the end can be prepared (S100).

[0075] Next, the well plate (100) can be fixed by being seated on the seating portion (320) of the lower fixed frame (300) (S200).

[0076] Next, the pillar plate (200) can be fixed to the upper fixed frame (400) (S300). Specifically, the pillar plate (200) can be placed on the step (412) by passing through the through hole (411) of the upper fixed frame (400) so that the pillar end in which the organoid is dispensed faces the lower fixed frame (300), and then the cover portion (420) can be placed on the upper surface of the pillar plate (200) placed on the step (412) and then fixedly coupled with the upper support plate (410).

[0077] Next, the actuator (500) moves the upper fixed frame (400) up and down according to preset stroke conditions, thereby repeatedly connecting and separating the pillar plate (200) from the well plate (100) to enable dynamic culture (S400). At this time, when the actuator (500) controls the up and down movement, the organoid (20) dispensed at the end of the pillar plate (200) can be moved up and down while immersed in the culture medium (10).

[0078] During dynamic cell culture in the S400 stage, the vertical movement control of the actuator (500) can be continuously repeated vertically by being driven based on preset stroke conditions (stroke length, stroke speed, etc.) during the culture time.

[0079] Alternatively, dynamic culture may be performed by moving the actuator (500) up and down at a preset cycle. That is, after moving up and down a preset number of times under preset stroke conditions during the culture time, the pillar plate (200) may be immersed in the well plate (100) and cultured for a set time, and the step of moving the actuator (500) up and down and then immersing may be repeated.

[0080] Alternatively, the initial actuator (500) may be moved up and down a preset number of times under preset stroke conditions during the initial culture time, and then the pillar plate (200) may be immersed in the well plate (100) for culture.

[0081] As such, the dynamic cell culture platform and dynamic cell culture method according to various embodiments of the present invention can improve the efficiency of organoid culture dispensed at the end of the pillar by generating fluid flow of the culture medium contained in the well through the repetitive up-and-down movement of the well-pillar plate.

[0083] It goes without saying that various embodiments of the present invention may be combined with one or more others to form a new embodiment.

[0084] A dynamic cell culture platform according to various embodiments of the present invention may include a lower fixing frame for fixing a well plate, an upper fixing frame for fixing a pillar plate, and an actuator having one end fixed to the lower fixing frame and the other end fixed to the upper fixing frame, which repeatedly controls the upper fixing frame to move up and down to repeatedly move the pillar plate in a direction of coupling to and separating from the well plate.

[0085] According to another feature of the present invention, the well plate contains a culture medium, and an organoid is dispensed at the end of the pillar plate, and when the actuator controls the vertical movement, the organoid dispensed at the end of the pillar plate can be moved vertically while immersed in the culture medium.

[0086] According to another feature of the present invention, the stroke length (S) of the actuator during vertical movement can be calculated by the following mathematical formula.

[0087] ,

[0088] The above is the immersion area media volume when the end of the above pillar plate is coupled to the well plate at the maximum depth, ...is the immersion area media volume when the end of the pillar plate is joined to the well plate at a minimum depth, and W is the well width, D is the pillar diameter, and L is the pillar length.

[0089] According to another feature of the present invention, the lower fixed frame may include a lower support plate and a seating portion formed with a groove that is spaced apart from the lower support plate at a predetermined interval and supports the well plate.

[0090] According to another feature of the present invention, the upper fixing frame may include a through hole through which the pillar of the pillar plate passes at a position corresponding to the seating portion, an upper support plate having a step formed therein to which the edge region of the pillar plate is caught, and a cover portion that is stacked on the upper part of the pillar plate that is engaged with the step and is seated and coupled to the step to fix the pillar plate.

[0091] According to another feature of the present invention, the device may further include a plurality of movable guide members, each comprising a guide shaft, the other end of which is fixed to the lower fixed frame and the other end of which is extended through the upper fixed frame, and a guide pipe into which the guide shaft is inserted and the other end of which is fixedly coupled to the upper fixed frame and slides along the guide shaft when the actuator is driven.

[0093] A dynamic cell culture method of a dynamic cell culture platform according to various embodiments of the present invention, comprising a lower fixing frame for fixing a well plate, an upper fixing frame for fixing a pillar plate, and an actuator having one end fixed to the lower fixing frame and the other end fixed to the upper fixing frame and controlling the up-and-down movement of the upper fixing frame, may include the steps of: (a) preparing a well plate containing a culture medium and a pillar plate having an organoid dispensed at one end; (b) fixing the well plate to the lower fixing frame; (c) fixing the pillar plate to the upper fixing frame; and (d) culturing the organoid while controlling the attachment and separation of the pillar plate to the well plate by the actuator repeatedly moving the upper fixing frame up and down.

[0095] Although embodiments of the present invention have been described in more detail with reference to the attached drawings, the present invention is not necessarily limited to these embodiments and may be modified in various ways within the scope of the technical spirit of the present invention. Accordingly, the embodiments disclosed in the present invention are intended to explain, not limit, the technical spirit of the present invention, and the scope of the technical spirit of the present invention is not limited by these embodiments. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. The scope of protection of the present invention shall be interpreted by the claims below, and all technical spirits within an equivalent scope shall be interpreted as being included within the scope of rights of the present invention. Explanation of the symbols

[0097] 100 : Well plate 200 : Pillar Plate 210 : Plate 220 : Pillar 10 : Culture solution 20: Organoids 300 : Lower fixed frame 310 : Lower support plate 320 : Seating part 321 : Settling Home 400 : Upper fixed frame 410 : Upper support plate 411 : Through hole 412 : Step 500 : Actuator 600a to 600d: Moving guide section 610 : Guide axis 620 : Guide pipe 630 : Joint

Claims

Claim 1 A dynamic cell culture platform comprising: a lower fixing frame for fixing a well plate; an upper fixing frame for fixing a pillar plate; and an actuator, one end of which is fixed to the lower fixing frame and the other end of which is fixed to the upper fixing frame, and which repeatedly controls the upper fixing frame to move up and down to cause the pillar plate to move repeatedly in a direction of coupling to and separating from the well plate. Claim 2 A dynamic cell culture platform according to claim 1, wherein the well plate contains a culture medium, an organoid is dispensed at the end of the pillar plate, and when the actuator controls the vertical movement, the organoid dispensed at the end of the pillar plate moves vertically while immersed in the culture medium. Claim 3 In claim 1, the stroke length (S) during vertical movement of the actuator is calculated by the following mathematical formula, and , The above is the immersion area media volume when the end of the above pillar plate is coupled to the well plate at the maximum depth, A dynamic cell culture platform in which the immersion area media volume is formed when the end of the pillar plate is joined to the well plate at a minimum depth, wherein W is the well width, D is the pillar diameter, and L is the pillar length. Claim 4 A dynamic cell culture platform according to claim 1, wherein the lower fixed frame comprises: a lower support plate; and a seating portion spaced apart from the lower support plate at a predetermined interval and having a groove formed therein for seating and supporting the well plate. Claim 5 In claim 4, the upper fixing frame comprises: an upper support plate having a through hole through which the pillar of the pillar plate passes at a position corresponding to the seating portion, and a step formed therein to which the edge region of the pillar plate is caught; and a cover portion stacked on the upper portion of the pillar plate that is engaged with the step and seated and coupled to the step to fix the pillar plate; a dynamic cell culture platform. Claim 6 A dynamic cell culture platform further comprising: a plurality of moving guide sections, wherein, in claim 5, the other end is fixed to the lower fixed frame and one end is extended through the upper fixed frame; and a guide pipe into which the guide axis is inserted and one end is fixedly coupled to the upper fixed frame and slides along the guide axis when the actuator is driven. Claim 7 A dynamic cell culture method comprising a dynamic cell culture platform including a lower fixing frame for fixing a well plate, an upper fixing frame for fixing a pillar plate, and an actuator having one end fixed to the lower fixing frame and the other end fixed to the upper fixing frame and controlling the up-and-down movement of the upper fixing frame, the method comprising: (a) preparing a well plate containing culture medium and a pillar plate having organoids dispensed at the end; (b) fixing the well plate to the lower fixing frame; (c) fixing the pillar plate to the upper fixing frame; and (d) dynamically culturing the pillar plate by controlling the coupling and separation of the pillar plate to the well plate by the actuator repeatedly moving the upper fixing frame up and down. Claim 8 In claim 7, when controlling the vertical movement of the actuator in step (d), the organoid dispensed at the end of the pillar plate moves vertically while immersed in the culture medium, a dynamic cell culture method. Claim 9 In claim 7, the stroke length (S) during vertical movement of the actuator is calculated by the following mathematical formula, and , The above is the immersion area media volume when the end of the above pillar plate is coupled to the well plate at the maximum depth, A dynamic cell culture method in which the immersion area media volume is formed when the end of the pillar plate is joined to the well plate at a minimum depth, wherein W is the well width, D is the pillar diameter, and L is the pillar length. Claim 10 A dynamic cell culture method according to claim 7, wherein the lower fixed frame comprises: a lower support plate; and a seating portion formed with a groove that is spaced apart from the lower support plate at a predetermined interval and supports the well plate. Claim 11 In claim 10, the upper fixing frame comprises: an upper support plate including a through hole through which the pillar of the pillar plate passes at a position corresponding to the seating portion, a first step on which the edge region of the pillar plate is engaged, and a second step forming a step difference with the first step; and a cover portion that is seated and coupled to the second step of the upper support plate and fixes the pillar plate engaged and coupled to the first step; a dynamic cell culture method.

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