Seasame Protein Isolate and Seasame Protein Isolate Extraction Method

Abstract

A sesame meal protein isolate and a method for extracting the sesame meal protein isolate are disclosed. A method for extracting the sesame meal protein isolate according to an embodiment of the present invention comprises: a step of lowering the fat content of the sesame meal using an edible basic first solution; a step of generating a protein precipitate of the sesame meal using an edible acidic second solution; and a step of treating the protein precipitate with the sesame meal protein isolate.

Description

Technology Field

[0001] The present invention relates to sesame meal, and more specifically to a sesame meal isolated protein and a method for extracting the sesame meal isolated protein. Background Technology

[0002] Sesame meal, a byproduct remaining after sesame oil extraction, is generally discarded except for use as animal feed. Consequently, issues such as additional disposal costs and environmental pollution have arisen. Fortunately, recent research has confirmed that sesame meal contains not only nutritional components such as protein, dietary fiber, and minerals, but also a large amount of lignans, which are well-known antioxidants. In particular, sesaminol glycosides, a type of lignan found in sesame meal, are known to possess various functionalities, including antioxidant properties and inhibition of brain cell damage. Furthermore, it has been revealed that sesame meal has a high protein content of over 55% per unit weight.

[0003] Consequently, significant interest is being focused on the utilization of sesame meal. However, there are difficulties in the industrial production of sesame meal extract. Furthermore, the use of various chemical substances in this industrial production can lead to environmental pollution. The problem to be solved

[0004] The present invention aims to provide a sesame meal isolated protein and a method for extracting the sesame meal isolated protein, which can be produced in large quantities in an environmentally friendly manner, of high-quality sesame meal isolated protein. means of solving the problem

[0005] A method for extracting isolated protein from sesame meal according to an embodiment of the present invention for solving the above technical problem comprises: a step of lowering the fat content of sesame meal to 5% or less using an edible basic first solution; a step of generating a protein precipitate of sesame meal with a fat content of 5% or less using an edible acidic second solution; and a step of treating the protein precipitate with isolated sesame meal protein.

[0006] The above fat content may be 2% or less.

[0007] The step of lowering the fat content of the sesame meal may include: a step of pulverizing the sesame meal; a step of mixing the pulverized sesame meal with distilled water and precipitating it; a step of mixing sodium bicarbonate with the distilled water to form the first solution having a pH of 7.5 to 8.5; and a step of stirring the first solution having a pH of 7.5 to 8.5 with an electronic stirrer at 70 to 80 ℃ for 80 to 100 minutes.

[0008] The step of forming the first solution may include the step of forming the hydrogen ion concentration of the first solution to pH 8.0±0.05 using 10% sodium bicarbonate.

[0009] The step of stirring the first solution may include stirring with an electronic stirrer at 75°C for 90 minutes.

[0010] The step of generating the protein precipitate may include: a step of obtaining a supernatant after centrifuging the first solution; a step of mixing citric acid with the obtained supernatant to form the second solution having a pH of 3.5 to 4.5; a step of stirring the second solution having a pH of 3.5 to 4.5 with an electronic stirrer at room temperature; and a step of centrifuging the stirred second solution to precipitate the protein precipitate.

[0011] The step of forming the second solution above may include the step of forming the hydrogen ion concentration of the second solution to pH 4.0±0.05 using 50% citric acid.

[0012] The step of treating the protein precipitate with sesame meal isolated protein may include: washing the protein precipitate with distilled water at least once; dispersing the washed protein precipitate in distilled water and then neutralizing it using sodium bicarbonate or citric acid; and drying the neutralized protein precipitate to form powdered sesame meal isolated protein.

[0013] A sesame meal isolated protein according to an embodiment of the present invention for solving the above technical problem is extracted through the steps of: lowering the fat content of sesame meal to 5% or less using an edible basic first solution; generating a protein precipitate of sesame meal with a fat content of 5% or less using an edible acidic second solution; and treating the protein precipitate with sesame meal isolated protein. Effects of the invention

[0014] According to the sesame meal isolated protein and the method for extracting sesame meal isolated protein according to the embodiment of the present invention, high-quality sesame meal isolated protein can be obtained with a simple process.

[0015] According to the sesame meal isolated protein and the method for extracting sesame meal isolated protein according to the embodiment of the present invention, environmental pollution is prevented when extracting sesame meal isolated protein, and the edible safety of the sesame meal isolated protein can be improved. Brief explanation of the drawing

[0016] FIG. 1 is a diagram showing a method for separating sesame meal protein according to an embodiment of the present invention. Figure 2 is a diagram showing the sesame meal isolated protein produced by the sesame meal isolated protein isolation method of Figure 1. FIG. 3 is a diagram showing the step of lowering the fat content of sesame meal according to an embodiment of the present invention. FIG. 4 is a diagram showing the step of generating a protein precipitate according to an embodiment of the present invention. Figure 5 is a table showing the protein purity of the sesame meal isolated protein according to an embodiment of the present invention. FIG. 6 is a diagram showing a method for separating sesame meal protein according to an embodiment of the present invention. Figure 7 is a graph showing the protein purity of the isolated protein of sesame meal according to an embodiment of the present invention. Specific details for implementing the invention

[0017] Hereinafter, specific embodiments of the present invention will be described with reference to the drawings. The following detailed description is provided to facilitate a comprehensive understanding of the methods, apparatuses, and / or systems described herein. However, this is merely illustrative and the present invention is not limited thereto.

[0018] In describing the embodiments of the present invention, detailed descriptions of known technologies related to the present invention are omitted if it is determined that such detailed descriptions may unnecessarily obscure the essence of the invention. Furthermore, the terms described below are defined in consideration of their functions within the present invention, and these may vary depending on the intentions or practices of the user or operator. Therefore, such definitions should be based on the content throughout this specification. Terms used in the detailed description are intended merely to describe the embodiments of the present invention and should not be limiting in any way. Unless explicitly stated otherwise, expressions in the singular form include the meaning of the plural form. In this description, expressions such as "include" or "comprise" are intended to refer to certain characteristics, numbers, steps, actions, elements, parts thereof, or combinations thereof, and should not be interpreted to exclude the existence or possibility of one or more other characteristics, numbers, steps, actions, elements, parts thereof, or combinations thereof other than those described.

[0019] Terms such as "first," "second," etc., may be used to describe various components, but said components should not be limited by said terms. These terms may be used for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be named the second component, and similarly, the second component may be named the first component.

[0020] FIG. 1 is a drawing showing a method (100) for extracting isolated protein from sesame meal according to an embodiment of the present invention, and FIG. 2 is a drawing showing isolated protein (200) from sesame meal produced by the method (100) for extracting isolated protein from sesame meal of FIG. 1.

[0021] Referring to FIGS. 1 and 2, a method for extracting isolated protein from sesame meal (100) according to an embodiment of the present invention comprises the steps of lowering the fat content of sesame meal using an edible basic first solution (s120), generating a protein precipitate of sesame meal using an edible acidic second solution (S140), and treating the protein precipitate with sesame meal isolated protein (200) (S160).

[0022] The first solution is a sodium bicarbonate solution with a hydrogen ion concentration of pH 7.5 to pH 8.5. That is, the first solution may be edible. Preferably, the first solution is a 10% sodium bicarbonate solution with a hydrogen ion concentration of pH 8.0 ± 0.05.

[0023] The second solution is a citric acid solution, and its hydrogen ion concentration may be pH 3.5 to pH 4.5. That is, the second solution may be edible. Preferably, the second solution is a 50% citric acid solution, and its hydrogen ion concentration may be pH 4.0 ± 0.05.

[0024] Thus, according to the sesame meal isolated protein extraction method (100) according to the embodiment of the present invention, a high-purity protein precipitate with reduced fat content is produced from sesame meal using each edible first solution and second solution (S120, S140), and the protein precipitate is treated as sesame meal isolated protein (200) (S160) to extract it, thereby preventing environmental pollution. In addition, the edibility of the sesame meal isolated protein (200) extracted by the sesame meal isolated protein extraction method (100) according to the embodiment of the present invention can be improved, and the utilization efficiency or utility of the sesame meal isolated protein (200) can be improved by extracting it with a high protein purity of 85% or more.

[0025] FIG. 3 is a drawing showing the step (S120) of lowering the fat content of sesame meal according to an embodiment of the present invention.

[0026] Referring to FIG. 3, the step (S120) of lowering the fat content of sesame meal according to an embodiment of the present invention may include the step of pulverizing the sesame meal (S122), the step of mixing the pulverized sesame meal with distilled water and precipitating it (S124), the step of mixing sodium bicarbonate with distilled water to form a first solution (S126), and the step of stirring the first solution (S128).

[0027] When mixing powdered sesame meal with distilled water, the ratio of sesame meal to distilled water may be approximately 1:30. Sodium bicarbonate may be used in the distilled water mixed with powdered sesame meal to adjust the hydrogen ion concentration of the first solution to pH 7.5 to pH 8.5 as described above, and preferably, the first solution may be formed as a 10% sodium bicarbonate solution with a hydrogen ion concentration of pH 8.0 ± 0.05. When stirring the first solution, an electronic stirrer may be used to stir at 70 to 80°C for 80 to 100 minutes. Preferably, the first solution may be stirred with an electronic stirrer at 75°C for 90 minutes.

[0028] Through this process, the fat content of sesame meal can be reduced. For example, the fat content of sesame meal can be reduced to 5% or less, preferably 2% or less.

[0029] FIG. 4 is a diagram showing the step (S140) of generating a protein precipitate according to an embodiment of the present invention.

[0030] Referring to FIG. 4, the step (S140) of generating a protein precipitate according to an embodiment of the present invention may include the step of obtaining an upper layer of a first solution (S142), the step of mixing citric acid with the obtained upper layer to form a second solution (S144), the step of stirring the second solution with an electronic stirrer at room temperature (S146), and the step of extracting a protein precipitate from the stirred second solution (S148).

[0031] The supernatant can be obtained after centrifuging the first solution. When citric acid is mixed with the obtained supernatant, the hydrogen ion concentration of the second solution can be set to pH 3.5 to pH 4.5 as described above, and preferably, a second solution with a hydrogen ion concentration of pH 4.0 ± 0.05 can be formed using 50% citric acid. When stirring the second solution, it can be stirred for 60 minutes using an electronic stirrer. The stirred second solution can be centrifuged to precipitate the protein precipitate of the second solution.

[0032] FIG. 5 is a diagram showing the step (S160) of treating a protein precipitate with sesame meal isolated protein (200) according to an embodiment of the present invention.

[0033] Referring to FIGS. 2 and FIGS. 5, the step (S160) of treating a protein precipitate with sesame meal isolated protein (200) according to an embodiment of the present invention may include the step of washing the protein precipitate with distilled water (S162), the step of dispersing the washed protein precipitate in distilled water and then neutralizing it (S164), and the step of drying the neutralized protein precipitate to form powdered sesame meal isolated protein (S166).

[0034] In washing the protein precipitate obtained through step S148 of FIG. 4, the washing process may be performed one or more times using distilled water. For example, the washing process may be performed three times using secondary distilled water. The washed protein precipitate may be dispersed using secondary distilled water. The protein precipitate dispersed in distilled water may be neutralized using sodium bicarbonate or citric acid. At this time, 10% sodium bicarbonate or 50% citric acid may be used. The neutralized protein precipitate may be processed by spray drying or freeze drying.

[0035] After the above process is carried out, the sesame meal isolated protein (200) can be extracted in a powdered form as shown in FIG. 2.

[0036] FIG. 6 is a diagram showing a method for extracting isolated protein from sesame meal according to an embodiment of the present invention.

[0037] Referring to FIGS. 2 and 6, a method (100) for isolating and extracting protein from sesame meal according to an embodiment of the present invention comprises the steps of: pulverizing sesame meal (S122-2); precipitating the pulverized sesame meal in distilled water (S124-2); adjusting the pH to 8.0±0.05 using 10% sodium bicarbonate (S126-2); stirring the mixture using an electronic stirrer at 75±1℃ for 90 minutes (S128-2); centrifuging the suspension reacted by stirring to obtain a supernatant (S142-2); adjusting the pH of the supernatant to 4.0±0.05 using 50% citric acid (S144-2); stirring the mixture using an electronic stirrer at room temperature for 90 minutes (S146-2); centrifuging the mixture to obtain a protein precipitate (S148-2); and washing the obtained protein precipitate once with distilled water. The method may include a step (S162-2), a step of dispersing the washed protein precipitate in distilled water and then adjusting the pH to 7.0±0.05 using 10% sodium bicarbonate or 50% citric acid (S164-2), and a step of drying the precipitate to form powdered sesame meal isolated protein (S166-2).

[0038] The specific conditions of each step of FIG. 6 may be changed as in the corresponding steps described above. Through the above process, the sesame meal isolated protein (200) extracted by the sesame meal isolated protein extraction method (100) according to an embodiment of the present invention can be extracted with a high protein purity of 85% or more.

[0039] FIG. 7 is a table showing experimental conditions for the protein purity of the sesame meal isolated protein (200) according to an embodiment of the present invention, and FIG. 8 is a graph showing the protein purity of the sesame meal isolated protein (200) according to an embodiment of the present invention.

[0040] Referring to FIGS. 7 and 8, experiments were conducted on the sesame meal isolated protein according to an embodiment of the present invention, extracted by the extraction method (100) of FIG. 1 described above, by dividing it into three types of sample groups. For example, experiments were conducted on the protein residue (Original), the mixture of protein residue and foam (Mix), and foam (Foam) extracted through FIG. 6. Each of the Original, Mix, and Foam sample groups was used in the experiment in a 10-fold diluted state, and the concentration (Con) was set to 240.8 mg / ml, 225.8 mg / ml, and 242.8 mg / ml for the Original, Mix, and Foam sample groups, respectively. In addition, the absorbance (Abs) for the Original, Mix, and Foam sample groups, respectively, was set to 0.281, 0.266, and 0.283.

[0041] In this case, 0.1114g, 0.1022g, and 0.1103g of sesame protein isolate (SPI) were extracted from the Original, Mix, and Foam sample groups, respectively, in 40ml of deionized water (DW). Through this, the protein purity for the Original, Mix, and Foam sample groups was confirmed to be 86%, 88%, and 88%, respectively, using the following mathematical formula.

[0042] Protein Purity (%) = {10 * (Con * 40) / SPI} * 100

[0043] Here, the “10” multiplied before () is to offset the experimental condition of 10-fold dilution, and the “100” multiplied after {} corresponds to the % unit.

[0044] Although representative embodiments of the present invention have been described in detail above, those skilled in the art will understand that various modifications can be made to the above-described embodiments without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be defined by the claims set forth below as well as equivalents thereof.

Claims

Claim 1 A method for extracting isolated sesame seed protein, comprising: a step of reducing the fat content of sesame seed meal to 5% or less using a first edible basic solution having a pH of 7.5 to 8.5; a step of generating a protein precipitate of sesame seed meal having a fat content of 5% or less using a second edible acidic solution having a pH of 3.5 to 4.5; and a step of treating the protein precipitate with sesame seed meal isolated protein having a protein purity of 85% or more; wherein the step of reducing the fat content of sesame seed meal comprises a step of stirring the first solution mixed with sesame seed meal with an electronic stirrer at 70 to 80 ℃ for 80 to 100 minutes. Claim 2 A method for extracting isolated protein from sesame meal according to claim 1, wherein the fat content is 2% or less. Claim 3 A method for extracting isolated protein from sesame seeds according to claim 1, wherein the step of lowering the fat content of the sesame seeds comprises: pulverizing the sesame seeds before stirring the first solution; mixing the pulverized sesame seeds with distilled water in a weight ratio of 1:30 and precipitating the mixture; and mixing sodium bicarbonate with the distilled water to form the first solution having a pH of 7.5 to 8.

5. Claim 4 A method for extracting isolated sesame meal protein according to claim 3, wherein the step of forming the first solution comprises the step of forming the hydrogen ion concentration of the first solution to pH 8.0±0.05 using 10% sodium bicarbonate. Claim 5 A method for extracting isolated sesame meal protein according to claim 3, wherein the step of stirring the first solution comprises stirring with an electronic stirrer at 75°C for 90 minutes. Claim 6 A method for extracting protein separated from sesame meal according to claim 1, wherein the step of generating the protein precipitate comprises: a step of obtaining a supernatant after centrifuging the first solution; a step of mixing citric acid with the obtained supernatant to form the second solution having a pH of 3.5 to 4.5; a step of stirring the second solution having a pH of 3.5 to 4.5 with an electronic stirrer at room temperature; and a step of centrifuging the stirred second solution to precipitate the protein precipitate. Claim 7 A method for extracting isolated sesame meal protein according to claim 6, wherein the step of forming the second solution comprises the step of forming the hydrogen ion concentration of the second solution to pH 4.0±0.05 using 50% citric acid. Claim 8 A method for extracting sesame meal isolated protein according to claim 1, wherein the step of treating the protein precipitate with sesame meal isolated protein comprises: washing the protein precipitate with distilled water one or more times; dispersing the washed protein precipitate in distilled water and then neutralizing it using sodium bicarbonate or citric acid; and drying the neutralized protein precipitate to form powdered sesame meal isolated protein. Claim 9 Low-fat sesame meal isolated protein with improved edible stability and protein purity of 85% or more, extracted by the sesame meal isolated protein extraction method of claim 1.

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