Method for inducing reprogramming of alveolar type 1 cell into alveolar type 2 cell using Dasatinib

Dasatinib-based medium composition facilitates the reprogramming of type 1 alveolar cells into type 2 alveolar cells within airway organoids, addressing the scarcity and culturing challenges, enabling efficient production of physiologically active type 2 alveolar cells.

KR102993487B1Active Publication Date: 2026-07-21THE CATHOLIC UNIV OF KOREA IND ACADEMIC COOP FOUND
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Patent Information

Authority / Receiving Office
KR · KR
Patent Type
Patents
Current Assignee / Owner
THE CATHOLIC UNIV OF KOREA IND ACADEMIC COOP FOUND
Filing Date
2023-09-15
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

The limited availability and difficulty in culturing type 2 alveolar cells (AT2 cells) due to their tendency to adopt a phenotype similar to type 1 alveolar cells (AT1 cells) in 2D culture, making it challenging to obtain sufficient numbers for research and applications.

Method used

A culture medium composition containing dasatinib, a tyrosine kinase inhibitor, is used to reprogram type 1 alveolar cells (AT1 cells) into type 2 alveolar cells (AT2 cells) within airway organoids, bypassing the need for overexpression of signaling proteins and transcription factors via viruses.

Benefits of technology

Efficient production of functional type 2 alveolar cells by directly reprogramming type 1 alveolar cells within organoids, overcoming the limitations of traditional methods and ensuring cells with physiological activity similar to actual tissue.

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Abstract

The present invention relates to a composition for inducing the reprogramming of type 1 alveolar cells into type 2 alveolar cells, comprising dasatinib as an active ingredient. According to the present invention, by reprogramming AT1 cells into AT2 cells using dasatinib, it is possible to efficiently secure AT2 cells, which were difficult to obtain in sufficient numbers. Furthermore, it is expected that cells with physiological activity similar to actual tissues can be secured by reprogramming AT1 cells into AT2 cells within the organoid while culturing the organoid itself, rather than culturing single cells.
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