A method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials

Abstract

A method (10) to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials is provided The method includes filtering a paste of a plurality of processed leaves and aloe-vera gel to obtain a first extract and a second extract respectively. The method also includes stirring, by a stirrer, the first extract and an aqueous potassium iodide solution to obtain a first solution. The method also includes stirring, by the stirrer, the first solution with an aqueous silver nitrate solution to obtain a colloidal solution. The method also includes centrifuging, by a centrifuge, the colloidal solution to obtain a precipitate. The method further includes dispersing, by an ultrasonicator, the precipitate to obtain the nanomaterial substance. The method also includes blending, by a blender, the nanomaterial substance and the second extract to obtain the nanomaterial composite, thereby preparing the nanomaterial composite with antibacterial properties using the plurality of biomaterials.

Description

[0001] A METHOD TO PREPARE A NANOMATERIAL COMPOSITE WITH ANTIBACTERIAL PROPERTIES USING A PLURALITY OF BIOMATERIALS

[0002] EARLIEST PRIORITY DATE

[0003] This Application claims priority from a Complete patent application filed in India having Patent Application No. 202331061412, filed on 12th day of September 2023, and titled “A METHOD TO PREPARE A NANOMATERIAL COMPOSITE WITH ANTIBACTERIAL PROPERTIES USING A PLURALITY OF BIOMATERIALS”.

[0004] FIELD OF INVENTION

[0005] Embodiments of the present disclosure relate to a field of antimicrobial preparations and more particularly to a method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials.

[0006] BACKGROUND

[0007] Bacteria are single-celled microorganisms present in various environments such as soil, water, air, and within living beings. The bacteria are classified into multiple categories based on shape, cell wall composition, metabolism, habitat, lifestyle, nutritional requirement, and temperature sensitivity. There exists a plurality of harmful effects associated with the bacteria. The plurality of harmful effects include diseases, destruction of crops, spoilage of foods, deterioration of materials, biofouling and water pollution.

[0008] Antibacterial substances are used to inhibit the bacteria. The antibacterial substances manufactured through chemical synthesis are known as artificial antibacterial substances. The artificial antibacterial substances develop antibiotic resistance in bacteria, rendering previously effective antibiotics useless and limiting treatment options for bacterial infections. Also, broad- spectrum effect of the artificial antibacterial substances eliminates the bacteria, which are harmful as well as harmless, thereby affecting natural balance of the bacteria in a target location. Further, the artificial antibacterial substances cause side effects such as allergies, digestive issues, and secondary infections. Furthermore, the artificial antibacterial substances cause environmental contamination by causing infiltration of the bacteria having antibiotic resistance into the environment. Also, the deteriorating efficacy and durability of the artificial antibacterial substances over time is another concern.

[0009] Hence, there is a need for an improved method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials to address the aforementioned issue(s).

[0010] OBJECTIVE OF THE INVENTION

[0011] An objective of the invention is to provide a method to prepare an environmentally friendly nanomaterial composite with antibacterial properties; and

[0012] Another objective of the invention is to prepare the nanomaterial composite which is durable and capable of retaining efficacy over time.

[0013] BRIEF DESCRIPTION

[0014] In accordance with an embodiment of the present disclosure, a method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials is provided. The method includes filtering a paste of a plurality of processed leaves and aloe-vera gel to obtain a first extract and a second extract respectively. The method also includes stirring, by a stirrer, the first extract and an aqueous potassium iodide solution to obtain a first solution. The method also includes stirring, by the stirrer, the first solution with an aqueous silver nitrate solution to obtain a colloidal solution. The method also includes centrifuging, by a centrifuge, the colloidal solution to obtain a precipitate. The method further includes dispersing, by an ultrasonicator, the precipitate to obtain the nanomaterial substance. The method also includes blending, by a blender, the nanomaterial substance and the second extract to obtain the nanomaterial composite, thereby preparing the nanomaterial composite with antibacterial properties using the plurality of biomaterials.

[0015] To further clarify the advantages and features of the present disclosure, a more explicit description of the disclosure will follow by reference to specific embodiments thereof, which are illustrated in the appended figures. It is to be appreciated that these figures depict typical embodiments of the disclosure and are therefore not to be considered limiting in scope. The disclosure will be described and explained with additional details with the appended figures.

[0016] BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The disclosure will be described and explained with additional specificity and detail with the accompanying figures in which:

[0018] FIG. 1 is a flow chart representing the steps involved in a method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials in accordance with an embodiment of the present disclosure;

[0019] FIG. 2 is a graphical representation of percentage of inhibition of k-pneumoniae bacteria with respect to various concentrations of the nanomaterial composite in accordance with an embodiment of the present disclosure; and

[0020] FIG. 3 is a graphical representation of the percentage of inhibition of S -pyogenes with respect to various concentrations of the nanomaterial composite in accordance with an embodiment of the present disclosure.

[0021] Further, those skilled in the art will appreciate that elements in the figures are illustrated for simplicity and may not have necessarily been drawn to scale. Furthermore, in terms of the construction of the device, one or more components of the device may have been represented in the figures by conventional symbols, and the figures may show only those specific details that are pertinent to understanding the embodiments of the present disclosure so as not to obscure the figures with details that will be readily apparent to those skilled in the art having the benefit of the description herein.

[0022] DETAILED DESCRIPTION

[0023] To promote an understanding of the principles of the disclosure, reference will now be made to the embodiment illustrated in the figures and specific language will be used to describe them. It will nevertheless be understood that no limitation of the scope of the disclosure is thereby intended. Such alterations and further modifications in the illustrated system, and such further applications of the principles of the disclosure as would normally occur to those skilled in the art are to be construed as being within the scope of the present disclosure. The terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process or method that comprises a list of steps does not include only those steps but may include other steps not expressly listed or inherent to such a process or method. Similarly, one or more devices or sub-systems or elements or structures or components preceded by "comprises... a" does not, without more constraints, preclude the existence of other devices, sub-systems, elements, structures, components, additional devices, additional sub-systems, additional elements, additional structures, or additional components. Appearances of the phrase "in an embodiment", "in another embodiment" and similar language throughout this specification may, but not necessarily do, all refer to the same embodiment.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this disclosure belongs. The system, methods, and examples provided herein are only illustrative and not intended to be limiting.

[0025] In the following specification and the claims, reference will be made to a number of terms, which shall be defined to have the following meanings. The singular forms “a”, “an”, and “the” include plural references unless the context clearly dictates otherwise.

[0026] Embodiments of the present disclosure relate to a method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials. The method includes filtering a paste of a plurality of processed leaves and aloe-vera gel to obtain a first extract and a second extract respectively. The method also includes stirring, by a stirrer, the first extract and an aqueous potassium iodide solution to obtain a first solution. The method also includes stirring, by the stirrer, the first solution with an aqueous silver nitrate solution to obtain a colloidal solution. The method also includes centrifuging, by a centrifuge, the colloidal solution to obtain a precipitate. The method further includes dispersing, by an ultrasonicator, the precipitate to obtain the nanomaterial substance. The method also includes blending, by a blender, the nanomaterial substance and the second extract to obtain the nanomaterial composite, thereby preparing the nanomaterial composite with antibacterial properties using the plurality of biomaterials. FIG. 1 is a flow chart representing the steps involved in a method (10) to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials in accordance with an embodiment of the present disclosure. The method (10) includes filtering a paste of a plurality of processed leaves and aloe-vera gel to obtain a first extract and a second extract respectively in step 20. In one embodiment, the plurality of processed leaves may include at least one of hygrophila auriculata, ocimum sanctum, centella asiatica, azadirachta indica, calendula officinalis, mentha x piperita, withania somnifera, curcuma longa, bacopa monnieri, emblica officinalis, bacopa monnieri, and bryophyllum pinnatum. In such an embodiment, the plurality of processed leaves may be airdried.

[0027] Further, in some embodiments, filtering the aloe-vera gel and the paste of a plurality of processed leaves may include filtering the aloe-vera gel and the paste of the plurality of processed leaves utilizing at least one of a suction filter and a double layer strainer. In such an embodiment, the aloe-vera gel may be in a frozen form. In a specific embodiment, the paste of the plurality of processed leaves may be made using a mortar and pestle. In some embodiments, the aloe-vera gel may be separated from a plurality of aloe-vera leaves upon cleansing the plurality of aloe-vera leaves with water and drying the plurality of aloe-vera leaves overnight. In an exemplary embodiment, quantity of the paste of the plurality of processed leaves and the first extract may be 10 grams and 5 grams respectively.

[0028] The method (10) also includes stirring the first extract and an aqueous potassium iodide solution to obtain a first solution in step 30. In one embodiment, stirring the first extract and an aqueous potassium iodide solution to obtain a first solution includes stirring the first extract and an aqueous potassium iodide solution to obtain a first solution by a stirrer. In one embodiment, the stirrer may include a magnetic stirrer.

[0029] The method (10) also includes stirring the first solution with an aqueous silver nitrate solution to obtain a colloidal solution in step 40. In one embodiment, stirring the first solution with an aqueous silver nitrate solution to obtain a colloidal solution includes stirring the first solution with an aqueous silver nitrate solution to obtain a colloidal solution by the stirrer. In one embodiment, the aqueous potassium iodide solution and the aqueous silver nitrate solution may include a molarity of 0.2 each with respect to 10 milli liters of water. In some embodiments, stirring the first extract and an aqueous potassium iodide solution to obtain a first solution may include stirring 5 milligrams of the first extract and 100 milligrams of the aqueous potassium iodide solution.

[0030] The method (10) also includes centrifuging the colloidal solution to obtain a precipitate in step 50. In one embodiment, centrifuging the colloidal solution to obtain a precipitate includes centrifuging the colloidal solution to obtain a precipitate by a centrifuge. In one embodiment, centrifuging the colloidal solution may include centrifuging the colloidal solution at 10000 rotations per minute for at least 10 minutes. In an exemplary embodiment, the quantity of the precipitate obtained may be 1.22 grams.

[0031] The method (10) also includes dispersing the precipitate to obtain the nanomaterial substance in step 60. In one embodiment, dispersing the precipitate to obtain the nanomaterial substance includes dispersing the precipitate to obtain the nanomaterial substance by an ultrasonicator. In an exemplary embodiment, the precipitate may be dispersed in 20 milli liters of water to obtain the nanomaterial substance. In such an embodiment, concentration of the nanomaterial substance may be 0.061 grams per milli liters.

[0032] The method (10) also includes blending the nanomaterial substance and the second extract to obtain the nanomaterial composite, thereby preparing the nanomaterial composite with antibacterial properties using the plurality of biomaterials in step 70. In one embodiment, blending the nanomaterial substance and the second extract to obtain the nanomaterial composite includes blending the nanomaterial substance and the second extract to obtain the nanomaterial composite by a blender. In one embodiment, the nanomaterial composite may be edible.

[0033] Further, in some embodiments, the method (10) may include adding a preservative to the nanomaterial composite to prevent degradation of the nanomaterial composite. In such an embodiment, adding a preservative to the nanomaterial composite to prevent degradation of the nanomaterial composite may include adding predefined quantity of vitamin E, wherein the predefined quantity comprises 400 milli grams. In one embodiment, the nanomaterial composite may be mixed with hydrophobic polymers for enhancing durability and robustness of coatings made by the nanomaterial composite. In one embodiment, the composite nanomaterial may be blended with neem to inhibit bacteria causing skin diseases. FIG. 2 is a graphical representation of percentage of inhibition (80) of k-pneumoniae bacteria (90) with respect to various concentrations of the nanomaterial composite (100) in accordance with an embodiment of the present disclosure. From the graphical representation it may be observed that, the percentage of inhibition of the k-pneumoniae bacteria is increasing with respect to an increase in the concentrations of the nanomaterial composite. Concentrations of the nanomaterial composite (100) is provided in microgram per milli liters.

[0034] FIG. 3 is a graphical representation of the percentage of inhibition (80) of S-pyogenes (110) with respect to various concentrations of the nanomaterial composite in accordance with an embodiment of the present disclosure. From the graphical representation it may be observed that the percentage of inhibition of the S-pyogenes is increasing with respect to an increase in the concentrations of the nanomaterial composite. Concentrations of the nanomaterial composite (100) is provided in microgram per milli liters.

[0035] Various embodiments of the method to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials described above enable various advantages. The nanomaterial composite is capable of inhibiting gram positive bacteria and gram negative bacteria effectively, thereby reducing harmful effects of the bacteria. The nanomaterial composite prepared using the plurality of biomaterials prevents development of antibiotic resistance in the bacteria, thereby ensuring effecting treatment by preserving current antibiotics. By preventing the development of antibiotic resistance in the bacteria, the nanomaterial composite is capable of preventing the environmental contamination caused by the proliferation of the bacteria having antibiotic resistance into the environment.

[0036] Further, the nanomaterial composite showcases narrow spectrum effect thereby ensuring inhibition of the harmful bacteria, thereby preserving the natural balance of the bacteria at the target location. The nanomaterial composite does not have any side effects as compared with the artificial antibacterial substances. Further, the nanomaterial composite has superior efficacy and durability compared with the artificial antibacterial substances. The nanomaterial composite is edible, thereby making the nanomaterial suitable for oral administration. Also, the nanomaterial is capable of being applied on the target location as layers, thereby making the nanomaterial composite suitable for coating various surfaces such as food packaging materials, interiors of space station, hospital equipments and the like.

[0037] It will be understood by those skilled in the art that the foregoing general description and the following detailed description are exemplary and explanatory of the disclosure and are not intended to be restrictive thereof. While specific language has been used to describe the disclosure, any limitations arising on account of the same are not intended.

[0038] The figures and the foregoing description give examples of embodiments. Those skilled in the art will appreciate that one or more of the described elements may well be combined into a single functional element. Alternatively, certain elements may be split into multiple functional elements. Elements from one embodiment may be added to another embodiment. For example, the order of processes described herein may be changed and is not limited to the manner described herein. Moreover, the actions of any flow diagram need not be implemented in the order shown; nor do all the acts need to be necessarily performed. Also, those acts that are not dependent on other acts may be performed in parallel with the other acts. The scope of embodiments is by no means limited by these specific examples.

Claims

AIM:

1. A method (10) to prepare a nanomaterial composite with antibacterial properties using a plurality of biomaterials comprising: characterized in that: filtering a paste of a plurality of processed leaves and aloe-vera gel to obtain a first extract and a second extract respectively; (20) stirring, by a stirrer, the first extract and an aqueous potassium iodide solution to obtain a first solution; (30) stirring, by the stirrer, the first solution with an aqueous silver nitrate solution to obtain a colloidal solution; (40) centrifuging, by a centrifuge, the colloidal solution to obtain a precipitate; (50) dispersing, by an ultrasonicator, the precipitate to obtain the nanomaterial substance; (60) and blending, by a blender, the nanomaterial substance and the second extract to obtain the nanomaterial composite, thereby preparing the nanomaterial composite with antibacterial properties using the plurality of biomaterials. (70)2. The method (10) as claimed in claim 1, wherein the plurality of processed leaves comprises at least one of hygrophila auriculata, ocimum sanctum, centella asiatica, azadirachta indica, calendula officinalis, mentha x piperita, withania somnifera, curcuma longa, bacopa monnieri, emblica officinalis, bacopa monnieri, and bryophyllum pinnatum, wherein the plurality of processed leaves are airdried.

3. The method (10) as claimed in claim 1, wherein filtering the aloe-vera gel and the paste of the plurality of processed leaves comprises filtering the aloe-vera gel and the paste of the plurality of processed leaves utilizing at least one of a suction filter and a double layer strainer, wherein the aloe-vera gel is in frozen form.

4. The method (10) as claimed in claim 1, wherein the stirrer comprises a magnetic stirrer.

5. The method (10) as claimed in claim 1, wherein the aqueous potassium iodide solution and the aqueous silver nitrate solution comprises a molarity of 0.2 each with respect to 10 milli liters of water.

6. The method (10) as claimed in claim 1, wherein centrifuging the colloidal solution comprises centrifuging the colloidal solution at 10000 rotations per minute for at least 10 minutes.

7. The method (10) as claimed in claim 1, wherein stirring the first solution with an aqueous silver nitrate solution to obtain a colloidal solution comprises stirring the first solution with an aqueous silver nitrate solution for at least 10 minutes.

8. The method (10) as claimed in claim 1, wherein stirring the first extract and an aqueous potassium iodide solution to obtain a first solution comprises stirring 5 milligrams of the first extract and 100 milligrams of the aqueous potassium iodide solution.

9. The method (10) as claimed in claim 1, wherein the nanomaterial composite is edible.

10. The method (10) as claimed in claim 1, comprising adding a preservative to the nanomaterial composite to prevent degradation of the nanomaterial composite comprises adding predefined quantity of vitamin E, wherein the predefined quantity comprises 400 milli grams.

Images