Use of genes NAT1 and bhlh110 in improving high-temperature resistance of rice

By using gene editing technology to mutate the rice NAT1 gene, the inhibition of bHLH110 was released, wax synthesis was promoted, and the problem of insufficient high temperature resistance of rice was solved, achieving significant improvement in high temperature resistance and agronomic traits.

WO2025190166A1 Publication Date: 2025-09-18ZHEJIANG UNIV
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Patent Information

Application Number
PCT/CN2025/081235
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-03-13
Filing Date
2025-03-07
Publication Date
2025-09-18

AI Technical Summary

Technical Problem

Existing technologies make it difficult to effectively improve rice's resistance to high temperatures, resulting in a significant decline in grain production due to climate change.

Method used

By using gene editing technology to mutate the NAT1 gene in rice, the inhibitory effect on bHLH110 is released, the expression of wax synthesis genes CER1 and CER1L is promoted, the wax thickness is increased, and thus the high temperature resistance of rice is enhanced.

Benefits of technology

It significantly improved the high temperature resistance of rice, including the survival rate of seedlings and the improvement of agronomic traits in the reproductive period, especially significantly improved the fruit set rate and yield under high temperature conditions.

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Abstract

Disclosed in the present invention is a use of genes NAT1 and bHLH110 in improving high-temperature resistance of rice, for use in using gene editing technology to obtain a rice material having enhanced high-temperature resistance. The base sequence of a rice gene NAT1 is as shown in SEQ ID NO. 1, and the base sequence of a gene bHLH110 is as shown in SEQ ID NO. 4. A base of a rice gene NAT1 of rice is replaced, deleted or inserted through gene editing technology to release inhibition on the rice gene bHLH110, and the expression of wax synthesis genes CER1 and CER1L is promoted by the rice gene bHLH110 to enhance the high-temperature resistance of a plant, thereby preparing a rice material having enhanced high-temperature resistance. According to the present invention, a NAT1 mutant rice material Nipponbare obtained by using CRISPR-Cas9 gene editing technology has remarkably enhanced high-temperature resistance, including an improved survival rate in a seedling stage and improved agronomic traits in a reproductive stage.
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