Novel promoter and use thereof

A novel polynucleotide with promoter activity addresses the need for high expression efficiency in diverse microorganisms, enhancing the production of target substances like amino acids by improving gene expression systems in Corynebacterium, Escherichia, and Bacillus species.

WO2025192843A1 Publication Date: 2025-09-18CJ CHEILJEDANG CORP
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Patent Information

Application Number
PCT/KR2024/095782
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-17
Filing Date
2024-05-17
Publication Date
2025-09-18

AI Technical Summary

Technical Problem

There is a need for a universal promoter system that exhibits high expression efficiency in various microorganisms, such as Escherichia, Corynebacterium, and Bacillus species, as existing promoters are not well-suited for these organisms and do not provide sufficient gene expression levels for producing target substances like amino acids and other compounds.

Method used

Development of a novel polynucleotide with promoter activity that can be used as a universal promoter for efficient gene expression in Corynebacterium, Escherichia, and Bacillus species, capable of enhancing the production of target products like amino acids and other biologically active substances.

Benefits of technology

The novel polynucleotide achieves significantly higher expression efficiency compared to existing promoters, enabling the production of target products with enhanced yields and regulatory control over gene expression.

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Abstract

The present application relates to a novel promoter and a method for producing a target product using same, wherein a polynucleotide according to one embodiment has promoter activity, when introduced into a microorganism can increase the expression and activity of an actively linked gene, and can be useful for efficiently producing a target product affected by the gene and the polynucleotide.
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