Composition for isolating muscle stem cells from skeletal muscle tissue, comprising enzyme combination and method for isolating muscle stem cells using same

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The use of a specific enzyme combination of collagenase I, II, IV, and trypsin optimizes muscle stem cell isolation from skeletal muscle, ensuring high purity and viability for cultured meat production by maintaining cell proliferation and differentiation potential.

WO2025249879A1 Publication Date: 2025-12-04HANWHA SOLUTIONS CORP

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Patent Information

Application Number: PCT/KR2025/007191
Authority / Receiving Office: WO · WO
Patent Type: Applications
Current Assignee / Owner
Priority Date: 2024-05-27
Filing Date: 2025-05-27
Publication Date: 2025-12-04

Application Information

Patent Timeline

27 May 2024

Priority

27 May 2025

Application

PCT/KR2025/007191

04 Dec 2025

Publication

WO2025249879A1

IPC: C12N5/077; A23L13/00; C12N9/64; C12N9/76

CPC: A23L13/00; C12N5/06; C12N9/64; C12N9/6427; C12N5/0659; C12N9/6489; C12Y304/21004; C12N2509/00

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Application Domain

Cell dissociation methods Skeletal/connective tissue cells

Technology Topics

Cultured meatMuscle stem cell

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AI Technical Summary

Technical Problem

Existing methods for isolating muscle stem cells from skeletal muscle tissue do not effectively maintain cell proliferation, purity, and differentiation potential, which are crucial for cultured meat production.

Method used

A composition comprising collagenase I, II, and IV, along with trypsin, is used to isolate muscle stem cells, optimizing enzyme concentrations and ratios to enhance cell viability, vitality, total cell number, and stemness without affecting proliferation.

Benefits of technology

The method achieves a high-purity muscle stem cell population with over 99% purity, increased cell viability and vitality, and enhanced differentiation potential, suitable for cultured meat production.

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Abstract

The present invention relates to: a composition for isolating muscle stem cells from skeletal muscle tissue, the composition comprising an enzyme combination; and a method for isolating muscle stem cells using same. More specifically, the present invention provides: a composition for isolating muscle stem cells from skeletal muscle tissue in which an optimal protease combination is used; a method for isolating muscle stem cells from skeletal muscle tissue using same; muscle stem cells isolated by said method; cultured meat obtained by culturing the muscle stem cells; and a food composition comprising same.

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