Cell culture vessels comprising dialysis membranes

The cell culture vessel with a porous dialysis membrane addresses the challenge of maintaining 3D cell cultures by selectively removing low molecular weight waste while retaining growth factors, improving efficiency and reducing medium change frequency.

WO2026054931A1 Publication Date: 2026-03-12CORNING INC
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Patent Information

Application Number
PCT/US2025/041075
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-09-05
Filing Date
2025-08-07
Publication Date
2026-03-12

AI Technical Summary

Technical Problem

Conventional cell culture apparatuses face challenges in maintaining efficient 3D cell culture environments due to the inability to selectively remove low molecular weight waste byproducts while retaining high molecular weight growth factors, leading to frequent medium changes that decrease efficiency and increase costs.

Method used

A cell culture vessel with a porous and liquid permeable dialysis membrane separating microcavity and media reservoir compartments, allowing diffusion of low molecular weight waste through microwells with controlled pore sizes and molecular weight cutoffs to maintain helpful byproducts within the microcavity compartment.

Benefits of technology

The solution enables efficient removal of toxic byproducts without disturbing the cell cultures, promoting continuous nutrient supply and reducing the frequency of medium changes, thereby enhancing cell growth efficiency and cost-effectiveness.

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Abstract

Disclosed herein are cell culture vessels including a microcavity compartment, a media reservoir compartment, and a dialysis membrane separating the microcavity compartment from the media reservoir compartment. The dialysis membrane may be porous and liquid permeable. The dialysis membrane may have a pore size greater than or equal to 1 nanometer and less than or equal to 100 nanometers. The dialysis membrane may include a plurality of microwells, each microwell having an interior surface defining an upper aperture and a nadir, the upper aperture facing the microcavity compartment.
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