Human papillomavirus detection

A multiplex qPCR assay for HPV16 and HPV18 nucleic acids provides rapid, sensitive, and specific detection in clinical pathology labs, overcoming the limitations of current OPSCC diagnostics by using optimized oligonucleotide sets for improved genomic coverage and cost-effective implementation.

WO2026072767A1 Publication Date: 2026-04-02THE CLEVELAND CLINIC FOUND
2 Cites 0 Cited by

Patent Information

Application Number
PCT/US2025/047892
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-09-30
Filing Date
2025-09-25
Publication Date
2026-04-02

AI Technical Summary

Technical Problem

Current diagnostics for oropharyngeal squamous cell carcinoma (OPSCC) associated with high-risk HPV lack sensitivity, specificity, and cost-effectiveness, and are impractical for clinical implementation, leading to inadequate management and screening, and existing HPV biomarkers are not optimized for rapid, flexible use in clinical pathology laboratories.

Method used

Development of a multiplex single-tube qPCR assay for detecting HPV16 and HPV18 nucleic acids using oligonucleotide sets with high sequence identity, allowing for rapid, sensitive, and specific detection in a single step, suitable for clinical pathology laboratories, without requiring digital PCR instruments.

Benefits of technology

The assay achieves high analytical sensitivity and specificity, with a rapid turnaround time, suitable for clinical pathology laboratories, and improved genomic coverage, addressing the limitations of existing diagnostics.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The present disclosure relates to compositions, kits, systems, and methods for detecting the presence or absence of human papillomavirus 16 (HPV16) or HPV18 nucleic acid (e.g., cell-free DNA) in a sample from a subject. In particular embodiments, a sample is contacted with at least one set of oligonucleotide having a forward primer, reverse primer, and a detectable labeled probe (e.g., for detecting PCR generated amplicons). In certain embodiments, the amount of HPV16 or HPV18 in the sample is quantitated (e.g., quantitative PCR is employed).
Need to check novelty before this filing date? Find Prior Art