New method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing

By using single-cell sequencing technology, Pdgfb and Tnfsf12 genes were screened as therapeutic targets for myocardial fibrosis in ischemic heart failure, which solves the problem that existing treatment strategies cannot improve cardiac function and provides a new method for controlling malignant fibrosis.

WO2026076708A1 Publication Date: 2026-04-16PKU HKUST SHENZHEN HONGKONG INSTITUTION
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Patent Information

Application Number
PCT/CN2024/124440
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Filing Date
2024-10-12
Publication Date
2026-04-16

AI Technical Summary

Technical Problem

Existing treatment strategies for ischemic heart failure have failed to effectively improve cardiac function, leading to a vicious cycle of myocardial fibrosis, and lack precise treatment targets.

Method used

By screening the myocardium of healthy mice and IHF mice using single-cell sequencing technology, we revealed the regulatory modules and pathways related to malignant fibrosis and screened the Pdgfb and Tnfsf12 genes as targets for the treatment of myocardial fibrosis in ischemic heart failure.

Benefits of technology

Precise screening of Pdgfb and Tnfsf12 genes as targets for the treatment of myocardial fibrosis in ischemic heart failure provides a new treatment strategy to control and reverse malignant fibrosis.

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Abstract

Provided is a method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing, which method comprises the following steps: S1, sample preparation; S2, construction of a single-cell expression matrix; S3, cell quality control; S4, cell type annotation; S5, cell communication analysis; and S6, co-expression network analysis. The provided method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing comprises performing single-cell sequencing on hearts of healthy mice and IHF mice, screening for cell types with significant differences in cardiac transcriptional profiles of the healthy mice and IHF mice, then exploring interaction characteristics of various types of cells in malignant fibrotic IHF hearts, revealing potential regulatory modules and pathways related to malignant myocardial fibrosis in single-cell expression data of IHF hearts, and performing screening to obtain Pdgfb and Tnfsf12 genes which can be used as therapeutic targets for treating myocardial fibrosis in ischemic heart failure.
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