NK cell culture system and method, and use thereof
By using a dual culture process involving irradiated trophoblast cells that have lost their proliferative capacity and a specific combination of cytokines, the problem of low NK cell proportion and difficulty in activation in peripheral blood was solved, achieving efficient expansion and high-purity NK cell culture, thus enhancing their cytotoxic activity.
WO2026081997A1 Publication Date: 2026-04-23SHENZHEN GENOCURY BIOTECH CO LTD
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Patent Information
- Application Number
- PCT/CN2025/127354
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-10-14
- Filing Date
- 2025-10-13
- Publication Date
- 2026-04-23
AI Technical Summary
Technical Problem
In existing technologies, NK cells are present in a small proportion in peripheral blood and are difficult to isolate and activate, which limits their expansion in clinical applications.
Method used
NK cells were expanded using a NK cell culture system containing irradiated, non-proliferating trophoblast cells and a specific combination of cytokines through a dual culture process using NK cell culture medium I and NK cell culture medium II.
Benefits of technology
It significantly improved the expansion efficiency and purity of NK cells, reduced the differences between different donors, and enhanced the killing activity of NK cells.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention relates to the field of immune cell therapy, and specifically relates to a method for expanding NK cells and use thereof. The method comprises using OK-432 in combination with irradiated feeder cells, such as K562 cells, to expand and culture NK cells in vitro.
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