Double-stranded DNA molecule
A closed circular double-stranded DNA molecule with a poly(dA) sequence is purified using oligo(dT) affinity chromatography, addressing inefficiencies in existing methods by achieving high specificity and purity while maintaining DNA integrity for cloning or expression.
WO2026114853A1PCT designated stage Publication Date: 2026-06-04SYNGOI TECHNOLOGIES SL
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- SYNGOI TECHNOLOGIES SL
- Filing Date
- 2025-11-25
- Publication Date
- 2026-06-04
Smart Images
Figure IMGF000045_0001 
Figure IMGF000046_0001 
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Abstract
The present invention relates to a closed circular double-stranded DNA molecule comprising a poly(dA) sequence from 25 to 200 nucleotide residues in length and an expression cassette, wherein the poly(dA) sequence is located at a position within the closed circular double-stranded DNA molecule such that, when the closed circular double-stranded DNA molecule is incubated under conditions suitable for transcription, the poly(dA) sequence is not transcribed; and when the closed circular double-stranded DNA molecule is incubated under conditions suitable for replication, the poly(dA) sequence does not interfere with the replication of the closed circular double-stranded DNA molecule. The invention also relates to a method for the isolation or purification of DNA molecules, to uses of poly(dA) and oligo(dT) in the isolation or purification of DNA molecules, and to isolation or purification kits.
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