Cosmetic product containing collagen and use of reverse micelle carrier in preparation of cosmetic product for improving intradermal retention of collagen

Abstract

A cosmetic product containing collagen and use of a reverse micelle carrier in the preparation of a cosmetic product for improving the intradermal retention of collagen. The cosmetic product comprises a collagen composite raw material. The collagen composite raw material comprises 53.55-80% by mass of grease, 15-60% by mass of a phospholipid, 0.5-1.5% by mass of an antioxidant, 0.1-2% by mass of water, and 0.01-0.5% by mass of collagen. The grease comprises any one of isopropyl myristate, isopropyl laurate, or isopropyl acetate. The phospholipid comprises lecithin, and the purity of lecithin is 90% or more. The present invention can increase the intradermal retention of collagen from the cosmetic product containing collagen, such that collagen penetrates into the epidermis or dermis and accumulates in that skin layer. The present invention greatly improves the real collagen-replenishing effect of the cosmetic product, which is conducive to restoring youthful skin.

Description

Cosmetics containing collagen and the application of reverse micelle transporters in the preparation of cosmetics that increase intradermal collagen retention.

[0001] Cross-reference to related applications

[0002] This application claims priority to Chinese Patent Application No. 2024118240357, filed on December 11, 2024, entitled "Cosmetics Containing Collagen, Application of Reverse Micellar Transporter in the Preparation of Cosmetics with Increased Intradermal Collagen Retention," the entire contents of which are incorporated herein by reference. Technical Field

[0003] This disclosure relates to the field of cosmetics, and more specifically, to a cosmetic containing collagen and the application of reverse micelle transporters in the preparation of cosmetics that increase the intradermal retention of collagen. Background Technology

[0004] Studies show that collagen synthesis in the human body slows down significantly at age 25, peaking at age 30 and then declining. Furthermore, modern unhealthy lifestyles and environmental damage further accelerate collagen loss, causing it to occur between the ages of 22 and 25. Therefore, supplementing collagen is key to youthful skin.

[0005] However, due to its structure, collagen is highly water-soluble and poorly oil-soluble, making it difficult to penetrate the natural lipid barrier formed by the stratum corneum. Collagen can only truly exert its effects when it penetrates into the epidermis or dermis and accumulates in that skin layer.

[0006] Known existing technologies, such as Chinese patent CN117417435A, typically use hydrophobic modifiers to modify collagen to enhance its oil solubility. However, the introduced organic reagents are prone to leaving residues and can easily cause skin problems. Summary of the Invention

[0007] The purpose of this disclosure is to provide a cosmetic containing collagen and the application of reverse micelle transporters in the preparation of cosmetics that increase the intradermal retention of collagen.

[0008] In a first aspect, this disclosure provides a cosmetic containing collagen, the cosmetic containing collagen including a collagen complex raw material; the collagen complex raw material forms a reverse micelle transporter structure;

[0009] By weight percentage, the collagen complex ingredients include: 53.55%–80% oils; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen.

[0010] Oils include any one of isopropyl myristate, isopropyl laurate, or isopropyl acetate;

[0011] Antioxidants include: butylated hydroxyanisole (BHA);

[0012] Phospholipids include lecithin, which has a purity of over 90%.

[0013] In the above technical solution, by using: 53.55%–80% oil; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen, a reverse micelle transporter structure containing collagen can be formed. This structure can simultaneously transport collagen to penetrate the natural lipid barrier formed by the stratum corneum and increase the amount of collagen retained in the skin. Applying this collagen raw material to cosmetics containing collagen can increase the amount of collagen retained in the skin, allowing collagen to penetrate into the epidermis or dermis and accumulate in that skin layer, thus better exerting its effects. This can greatly enhance the actual effect of cosmetics in supplementing collagen, which is beneficial to youthful skin.

[0014] In other embodiments of this disclosure, the viscosity of the collagen composite material is 30,000 Pa·s to 60,000 Pa·s.

[0015] In other embodiments of this disclosure, the pore size of the collagen composite material is approximately 300 nm to 500 nm.

[0016] Secondly, this disclosure provides the application of a reverse micelle transporter in the preparation of cosmetics that increase the intradermal retention of collagen.

[0017] The antimicelle transporter, by weight percentage, comprises: 53.55%–80% oils; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen.

[0018] Oils and fats include any one of isopropyl palmitate, isopropyl myristate, isopropyl laurate, or isopropyl acetate.

[0019] Antioxidants include any one of tert-butylhydroquinone, butylated hydroxyanisole, tocopheryl acetate, propyl gallate, or butylated hydroxytoluene;

[0020] Phospholipids include lecithin, which has a purity of over 90%.

[0021] In other embodiments of this disclosure, the viscosity of the reverse micelle carrier is 30000 Pa·s to 60000 Pa·s.

[0022] In other embodiments of this disclosure, the pore size of the reverse micelle carrier is approximately 300 nm to 500 nm.

[0023] In other embodiments of this disclosure, the preparation of the antimicelle transporter includes:

[0024] An oil-phase mixture was prepared by mixing phospholipids, oils, and antioxidants.

[0025] Aqueous mixtures were prepared by mixing collagen with water.

[0026] When the aqueous phase mixture is added dropwise to the oil phase mixture, a viscous reverse micelle structure is formed.

[0027] In the above technical solution, an aqueous mixture of collagen and water is added dropwise to an oil mixture of phospholipids and oils, forming a viscous reverse micelle structure. This viscous reverse micelle structure can act as a carrier for collagen, transporting it below the natural lipid barrier formed by the stratum corneum; simultaneously, this viscous reverse micelle structure can increase the amount of collagen retained in the skin; and by incorporating this collagen complex into cosmetics, the collagen-replenishing effect of cosmetics can be effectively improved.

[0028] In other embodiments of this disclosure, an aqueous phase mixture is dropped onto an oil phase mixture to form a viscous reverse micelle structure, comprising:

[0029] The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, with the viscosity controlled at 30000 Pa·s to 60000 Pa·s.

[0030] In other embodiments of this disclosure, collagen is mixed with water to prepare an aqueous mixture, comprising:

[0031] Collagen and water are mixed in a ratio of (0.1%–25%) to (75%–99.9%) by weight.

[0032] Optionally, an oil-phase mixture is prepared by mixing phospholipids, oils, and antioxidants, comprising:

[0033] Phospholipids, oils and antioxidants are mixed in a ratio of (15%–60%): (53.55%–80%): (0.5%–1.5%) by weight percentage.

[0034] In other embodiments of this disclosure, adding the aqueous phase mixture dropwise to the oil phase mixture includes:

[0035] Add 0.1% to 2% of the aqueous phase mixture dropwise to 98% to 99.9% of the oil phase mixture by mass percentage, and stir. Attached Figure Description

[0036] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the accompanying drawings used in the embodiments will be briefly described below. It should be understood that the following drawings only show some embodiments of this disclosure and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0037] Figure 1 is a transmission electron microscope image of the collagen composite raw material prepared in Example 1 of this disclosure;

[0038] Figure 2 is a transmission electron microscope image of the collagen composite raw material prepared in Example 2 of this disclosure;

[0039] Figure 3 is a transmission electron microscope image of the collagen composite raw material prepared in Example 3 of this disclosure. Detailed Implementation

[0040] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions in the embodiments of this disclosure will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0041] Research has shown that phospholipids can encapsulate collagen to form reverse micelle structures. These reverse micelle structures can simultaneously transport collagen and increase its retention in the skin. Applying this collagen ingredient to collagen-containing cosmetics can further enhance the retention of collagen in the skin. Collagen only exerts its full effect when it penetrates the epidermis or dermis and accumulates in that layer. This significantly improves the actual effectiveness of collagen supplementation and promotes youthful skin.

[0042] Based on this, the present disclosure provides a cosmetic containing collagen, which includes a collagen complex raw material; the collagen complex raw material forms a reverse micelle transporter structure.

[0043] By weight percentage, the collagen complex ingredients include: 53.55%–80% oils; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen.

[0044] Oils include any one of isopropyl myristate, isopropyl laurate, or isopropyl acetate;

[0045] Antioxidants include: butylated hydroxyanisole (BHA);

[0046] Phospholipids include lecithin, which has a purity of over 90%.

[0047] In the above technical solution, by using: 53.55%–80% oil; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen, a reverse micelle transporter structure containing collagen can be formed. This structure can simultaneously transport collagen to penetrate the natural lipid barrier formed by the stratum corneum and increase the amount of collagen retained in the skin. Applying this collagen raw material to cosmetics containing collagen can increase the amount of collagen retained in the skin, allowing collagen to penetrate into the epidermis or dermis and accumulate in that skin layer, thus better exerting its effects. This can greatly enhance the actual effect of cosmetics in supplementing collagen, which is beneficial to youthful skin.

[0048] For example, in some embodiments of this disclosure, the collagen complex raw material comprises, by weight percentage:

[0049] Oils containing 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 70%, 71%, 72%, 75%, 78%, 80%, or any two of the aforementioned values;

[0050] Antioxidant in amounts of 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, or any range between two of the aforementioned values;

[0051] Phospholipids in the range of 16%, 18%, 20%, 22%, 25%, 30%, 35%, 40%, 45%, 50%, 52%, 55%, 58%, 60%, or any two of the aforementioned values;

[0052] Water content of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.2%, 1.5%, 1.8%, 2%, or any two of the aforementioned values; and

[0053] Collagen content of 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, or any two of the aforementioned values.

[0054] Optionally, in some embodiments of this disclosure, the aforementioned oils include any one of isopropyl palmitate, isopropyl myristate, isopropyl laurate, or isopropyl acetate.

[0055] Optionally, in some embodiments of this disclosure, the antioxidants mentioned above include any one of tert-butylhydroquinone, butylated hydroxyanisole, tocopheryl acetate, propyl gallate, or butylated hydroxytoluene.

[0056] For example, in some embodiments of this disclosure, the oil is isopropyl palmitate; or in some embodiments of this disclosure, the oil is isopropyl myristate; or in some embodiments of this disclosure, the oil is isopropyl laurate; or in some embodiments of this disclosure, the oil is isopropyl acetate.

[0057] For example, in some embodiments of this disclosure, the antioxidant is tert-butylhydroquinone; or in some embodiments of this disclosure, the antioxidant is butylated hydroxyanisole; or in some embodiments of this disclosure, the antioxidant is tocopheryl acetate; or in some embodiments of this disclosure, the antioxidant is propyl gallate; or in some embodiments of this disclosure, the antioxidant is butylated hydroxytoluene.

[0058] In some embodiments of this disclosure, the phospholipids mentioned above include lecithin, and the purity of the lecithin is 90% or higher.

[0059] Further optionally, and exemplary, in some embodiments of this disclosure, the purity of the lecithin described above is 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%, or a range between any two of the aforementioned values.

[0060] Furthermore, in some embodiments of this disclosure, the viscosity of the collagen composite raw material is 30000 Pa·s to 60000 Pa·s.

[0061] When the viscosity is between 30,000 Pa·s and 60,000 Pa·s, the reverse micelles exhibit a moderate degree of structural compactness. This viscosity indicates that the interactions between the reverse micelles have reached a balance; they are neither too loose, leading to premature collagen release, nor too tight, hindering collagen contact with the skin. This viscosity helps control the release rate of collagen, allowing it to slowly and continuously penetrate the skin. If the viscosity is too low, collagen may be released all at once, failing to penetrate effectively; if the viscosity is too high, collagen release will be severely hindered, also impeding penetration. Collagen can better adapt to the intradermal environment, allowing it to remain in the skin for a longer period, thereby increasing intradermal retention.

[0062] Viscosity is one of the important physical properties of fluids. Fluid flow patterns can be divided into Newtonian fluids, where the required shear stress for flow does not change with the flow velocity, and non-Newtonian fluids, where the required shear stress for flow changes with the flow velocity. Pseudoplastic fluids, a type of non-Newtonian fluid, exhibit shear thinning characteristics.

[0063] In some embodiments of this disclosure, it is assumed that the collagen composite material is a non-Newtonian fluid, and its viscosity is tested as follows:

[0064] Before conducting sample viscosity measurements, a rotor model that matches the sample characteristics and estimated viscosity range must be carefully selected based on a preliminary assessment of the sample viscosity. The selected rotor is then precisely installed onto the viscometer's shaft. The container holding the sample is placed in a temperature-controlled environment, and this constant temperature is maintained. Once the sample temperature and the preset measurement temperature reach thermal equilibrium and remain stable for a period of time, the sample is carefully transferred to the viscometer's measuring cylinder using a professional pipette at a slow, steady, and controllable rate. The addition process continues until the sample level reaches and submerges the designated graduation mark on the rotor. If air bubbles are accidentally introduced, the addition process must be stopped immediately. The sample should be placed on a stable surface for 3-5 minutes, depending on the number and size of the air bubbles, allowing them to rise and escape naturally. After completing the sample loading and air bubble removal, turn on the viscometer. Based on the predetermined measurement plan and sample characteristics, accurately set the measurement speed on the viscometer's control panel. Once the data displayed on the viscometer's reading screen stabilizes, accurately record the viscosity value shown on the screen. Repeat this measurement operation three times strictly following this procedure. Exemplarily, in some embodiments of this disclosure, the viscosity of the collagen composite raw material is 30000 Pa·s, 35000 Pa·s, 40000 Pa·s, 42000 Pa·s, 45000 Pa·s, 48000 Pa·s, 50000 Pa·s, 52000 Pa·s, 55000 Pa·s, 58000 Pa·s, 60000 Pa·s, or a range between any two of the aforementioned values.

[0065] Secondly, some embodiments of this disclosure provide the application of an antimicelle transporter in the preparation of cosmetics that increase the intradermal retention of collagen.

[0066] Furthermore, in some embodiments of this disclosure, the antimicelle carrier comprises, by weight percentage: 53.55% to 80% oil; 15% to 60% phospholipid; 0.5% to 1.5% antioxidant; 0.1% to 2% water; and 0.01% to 0.5% collagen.

[0067] Furthermore, in some embodiments of this disclosure, the oil includes any one of isopropyl palmitate, isopropyl myristate, isopropyl laurate, or isopropyl acetate;

[0068] Furthermore, in some embodiments of this disclosure, the antioxidant includes any one of tert-butylhydroquinone, butylated hydroxyanisole, tocopheryl acetate, propyl gallate, or butylated hydroxytoluene;

[0069] Furthermore, in some embodiments of this disclosure, the phospholipids include lecithin, and the purity of the lecithin is 90% or higher.

[0070] Furthermore, in some embodiments of this disclosure, the viscosity of the reverse micelle carrier is 30000 Pa·s to 60000 Pa·s.

[0071] Furthermore, in some embodiments of this disclosure, the pore size of the reverse micelle carrier is approximately 300 nm to 500 nm.

[0072] Further optionally, in some embodiments of this disclosure, the collagen mentioned above may be recombinant collagen; for example, it may include any one of type I recombinant collagen, type II recombinant collagen, or type III recombinant collagen.

[0073] Further optionally, in some embodiments of this disclosure, the amino acid sequence of the recombinant collagen includes amino acids 950-1075 of type I collagen and amino acids 594-729 and 1193-1199 of type III collagen.

[0074] Furthermore, the aforementioned collagen can be obtained through commercial purchases.

[0075] For example, in some embodiments of this disclosure, the collagen mentioned above may be HydroCol-y recombinant collagen produced by Best Pharmaceutical (Guangzhou) Co., Ltd.

[0076] Furthermore, in some embodiments of this disclosure, the preparation of the antimicelle transporter includes:

[0077] A mixture of phospholipids and oil antioxidants was prepared to obtain an oil phase mixture.

[0078] Aqueous mixtures were prepared by mixing collagen with water.

[0079] When the aqueous phase mixture is added dropwise to the oil phase mixture, a viscous reverse micelle structure is formed.

[0080] In the above technical solution, by adding the aqueous phase mixture to the oil phase mixture to form a viscous reverse micelle structure, collagen can be transported simultaneously, and the amount of collagen retained in the skin can be increased. Applying this collagen raw material to cosmetics containing collagen can increase the amount of collagen retained in the skin. Collagen can only exert its effects better when it penetrates into the epidermis or dermis and accumulates in the skin layer; thus greatly enhancing the actual effect of collagen supplementation and promoting youthful skin.

[0081] Furthermore, in some embodiments of this disclosure, and in other embodiments thereof, the aqueous phase mixture is dropwise added to the oil phase mixture to form a viscous reverse micelle structure, including:

[0082] The aqueous phase mixture was added dropwise to the oil phase mixture, forming a viscous reverse micelle structure. The viscosity was controlled to be...

[0083] Furthermore, in some embodiments of this disclosure, the collagen is mixed with water to prepare an aqueous mixture, including:

[0084] Collagen and water are mixed in a ratio of (0.1% to 25%): (75% to 99.9%) by weight percentage.

[0085] Exemplarily, in some embodiments of this disclosure, collagen is mixed with water to prepare an aqueous mixture, including:

[0086] Collagen is mixed with water at the following percentages by weight: (0.1%, 0.2%, 0.3%, 0.5%, 0.6%, 0.7%, 0.8%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 20%, 25% or any two of the aforementioned values) or (75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.9% or any two of the aforementioned values).

[0087] Furthermore, in some embodiments of this disclosure, an oil-phase mixture is prepared by mixing phospholipids, oils, and antioxidants, comprising:

[0088] Phospholipids, oils and antioxidants are mixed in a ratio of (15%–60%): (53.55%–80%): (0.5%–1.5%) by weight percentage.

[0089] Exemplarily, in some embodiments of this disclosure, an oil phase mixture is prepared by mixing phospholipids, oils, and antioxidants, including:

[0090] By weight percentage, phospholipids, oils and antioxidants are mixed in the following proportions: (15%, 18%, 20%, 30%, 35%, 40%, 45%, 46%, 47%, 48%, 49%, 52%, 55%, 60% or any two of the aforementioned values): (55%, 58%, 60%, 62%, 65%, 70%, 75%, 78%, 80% or any two of the aforementioned values): (0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5% or any two of the aforementioned values).

[0091] Furthermore, in some embodiments of this disclosure, the aqueous phase mixture is added dropwise to the oil phase mixture, including:

[0092] Add 0.1%–2.0% of the aqueous phase mixture dropwise to the 98%–99.9% oil phase mixture by mass percentage, and stir.

[0093] Exemplarily, in some embodiments of this disclosure, adding an aqueous phase mixture dropwise to an oil phase mixture includes:

[0094] By mass percentage, add 0.1%, 0.2%, 0.5%, 1%, 1.1%, 1.2%, 1.3%, 1.5%, 1.6%, 1.7%, 1.8%, and 2.0% of the aqueous phase mixture dropwise to the 98%–99.9% oil phase mixture and stir.

[0095] In other optional embodiments of this disclosure, the aforementioned collagen-containing essential oil cosmetics may also include other applicable cosmetic bases commonly used in the art (at least one of essence water, essence lotion, and face cream), and the aforementioned collagen complex raw materials are compounded with the cosmetic base to form cosmetics.

[0096] Further optionally, in some embodiments of this disclosure, the amount of the aforementioned collagen complex raw material added in the cosmetic product is 0.01% to 30% by mass percentage; exemplaryly, in some embodiments of this disclosure, the amount of the aforementioned collagen complex raw material added in the cosmetic product is 0.01%, 0.02%, 0.05%, 0.08%, 0.1%, 0.2%, 0.5%, 1%, 2%, 3%, 4%, 5%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, 30%, or a range between any two of the aforementioned values.

[0097] For example, the formula of an essential oil cosmetic containing the aforementioned collagen complex is shown in Table 1 below:

[0098] Table 1

[0099] Further, alternatively, in some embodiments of this disclosure, the cosmetic matrix described above may include moisturizers, emulsifiers, thickeners, etc., commonly used in the art.

[0100] The features and performance of this disclosure will be further described in detail below with reference to embodiments:

[0101] Examples and Comparative Examples

[0102] A collagen composite raw material is provided, and the formulation of various embodiments and comparative examples is shown in Table 2;

[0103] Table 2

[0104] Preparation process:

[0105] Weigh each raw material according to the component proportions of each embodiment or comparative example in Table 2 above:

[0106] Each embodiment was prepared according to the following preparation method:

[0107] S1. Mix phospholipids, oils and antioxidants, heat and stir to form a homogeneous oil phase mixture;

[0108] S2. Mix and stir the various raw materials in the aqueous phase until a homogeneous aqueous mixture is formed;

[0109] S3. Add the uniform aqueous phase mixture obtained in step S2 dropwise to the uniform oil phase mixture obtained in step S2, and stir at room temperature until a viscous reverse micelle is formed.

[0110] The comparative example was prepared according to the following method:

[0111] S1. Mix and stir the various raw materials in the aqueous phase until a homogeneous aqueous mixture is formed.

[0112] Performance testing

[0113] 1. In vitro transdermal assay:

[0114] The samples prepared in the aforementioned examples and comparative examples were subjected to transdermal experiments on isolated porcine skin using the vertical Franz diffusion cell method.

[0115] Piglet skin was fixed in the receiving chamber and the supply chamber. 0.5 g of each collagen composite raw material from each embodiment and the collagen aqueous solution from Comparative Example 1 were placed in the supply chamber. PBS buffer solution (pH 7.4) was used as the receiving solution, and the mixture was stirred and diffused at 32°C. After 24 hours, the skin was removed, washed, and cut into small pieces. An appropriate amount of PBS buffer solution was added, and the mixture was shaken for half an hour. The supernatant was then collected for HPLC analysis to calculate the intradermal retention of collagen per unit area. The experimental results are shown in Table 3.

[0116] Table 3

[0117] The test results in Table 3 above show that:

[0118] Intradermal retention results showed:

[0119] Compared to the collagen aqueous solution provided in Comparative Example 1, the collagen composite raw materials provided in each embodiment showed a greater intradermal retention of collagen than those in Comparative Example 1; this indicates that the collagen composite raw materials provided in each embodiment can effectively increase the intradermal retention of collagen. The increase in intradermal retention of collagen in each embodiment compared to Comparative Example 1 ranged from 2.99 to 4 times.

[0120] Furthermore, comparing the various embodiments, it can be seen that the collagen complex provided in Example 1 resulted in the greatest intradermal retention of collagen, indicating that the collagen complex provided in Example 1 had the best effect on increasing intradermal collagen retention. This demonstrates that when oils, phospholipids, antioxidants, water, and collagen are mixed in a mass percentage ratio of 69.3%:28.5%:1.5%:1.35%:0.15%, the effect on increasing intradermal collagen retention is optimal. Compared to Comparative Example 1, Example 1 can increase intradermal collagen retention by more than four times.

[0121] The viscosity in Table 3 was measured using the viscosity test method described above. As can be seen from Table 3, the viscosity of the collagen complex increases with the increase of phospholipid content, while the viscosity of the collagen complex is not positively correlated with the amount retained in the skin. The present invention selects a relatively moderate viscosity range, which will be more conducive to promoting collagen penetration and increasing its retention in the skin.

[0122] Adding the above-mentioned collagen complex to cosmetics containing collagen can effectively increase the amount of collagen retained in the skin, thereby enhancing the collagen-supplementing effect of the cosmetics and maximizing their efficacy.

[0123] 2. Microstructure characterization

[0124] The microstructure of the collagen composite raw materials provided in Examples 1, 2, and 3 was observed using transmission electron microscopy.

[0125] 10 μl of each sample was dropped onto a copper grid and precipitated for 1 min. The floating liquid was then absorbed with filter paper. 10 μl of uranium acetate staining solution was dropped onto a copper grid and precipitated for 1 min. The floating liquid was then absorbed with filter paper. The samples were dried at room temperature for several minutes. Electron microscopy was performed at 80 kV-120 kV for detection and imaging, and the images were acquired and analyzed.

[0126] The transmission electron microscopy results are shown in Figures 1-3 of the instruction manual.

[0127] As can be seen from the transmission electron microscope images in Figures 1-3 of the instruction manual:

[0128] The collagen composite raw materials provided in Examples 1-3 exhibit a reverse micelle structure. The pore size of the collagen-loaded reverse micelle structure is approximately 300 nm to 500 nm. This nanoscale pore size structure is more conducive to carrying collagen into the skin and increasing the amount of collagen retained in the skin. This is because the skin has accessory channels such as hair follicles and sweat glands. The diameter of a hair follicle is approximately 50 to 100 μm, and the diameter of a sweat gland duct is approximately 10 to 20 μm. The 300 nm to 500 nm reverse micelle particle size is relatively small, allowing it to utilize the gaps around these accessory channels or aggregate within the channels. The size of endocytic vesicles in skin cells is typically around 100 to 300 nm. Although the 300 nm to 500 nm reverse micelle particle size is slightly larger than that of typical endocytic vesicles, it is still within the effective range that cells can recognize and take up. When the reverse micelle size is within this range, skin cells can take up the reverse micelles through some special endocytosis mechanisms; once collagen enters the skin, reverse micelles with a size of 300nm to 500nm can, to some extent, prevent collagen from being cleared away too quickly by the skin's internal metabolic processes.

[0129] The above description is merely a preferred embodiment of this disclosure and is not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure. Industrial applicability

[0130] This disclosure provides a cosmetic containing collagen that uses oils, phospholipids, antioxidants, water, and collagen to form a collagen complex, which then forms a reverse micelle transporter structure. This increases the amount of collagen retained in the skin, allowing collagen to penetrate into the epidermis or dermis and accumulate in that skin layer. This greatly enhances the actual effect of cosmetics in replenishing collagen and is beneficial for youthful skin.

Claims

1. A cosmetic containing collagen, characterized in that, The cosmetic containing collagen includes a collagen complex raw material; the collagen complex raw material forms a reverse micelle transporter structure; The collagen complex comprises, by weight percentage: 53.55%–80% oils; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen. The oil includes any one of isopropyl myristate, isopropyl laurate, or isopropyl acetate. The antioxidants include: butylated hydroxyanisole; The phospholipids include lecithin, and the purity of the lecithin is above 90%.

2. The cosmetic containing collagen according to claim 1, characterized in that, The viscosity of the collagen composite raw material is 30000 Pa·s to 60000 Pa·s.

3. The cosmetic containing collagen according to claim 1, characterized in that, The pore size of the collagen composite raw material is 300nm to 500nm.

4. The application of a reverse micelle transporter in the preparation of cosmetics that increase intradermal collagen retention, characterized in that, The reverse micelle transporter comprises, by weight percentage: 53.55%–80% oils; 15%–60% phospholipids; 0.5%–1.5% antioxidants; 0.1%–2% water; and 0.01%–0.5% collagen. The oil includes any one of isopropyl myristate, isopropyl laurate, or isopropyl acetate. The antioxidants include: butylated hydroxyanisole; The phospholipids include lecithin, and the purity of the lecithin is above 90%.

5. The application of the reverse micelle transporter according to claim 4 in the preparation of cosmetics that increase the intradermal retention of collagen, characterized in that, The viscosity of the reverse micelle carrier is 30000 Pa·s to 60000 Pa·s.

6. The application of the reverse micelle transporter according to claim 4 in the preparation of cosmetics that increase intradermal collagen retention, characterized in that, The pore size of the reverse micelle carrier is approximately 300 nm to 500 nm.

7. The application of the reverse micelle transporter according to claim 4 in the preparation of cosmetics that increase the intradermal retention of collagen, characterized in that, The preparation of the reverse micelle transporter includes: mixing phospholipids, oils and antioxidants to obtain an oil phase mixture; Aqueous mixtures were prepared by mixing collagen with water. The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure.

8. The application of the reverse micelle transporter according to claim 7 in the preparation of cosmetics that increase the intradermal retention of collagen, characterized in that, The step of adding the aqueous phase mixture dropwise to the oil phase mixture to form a viscous reverse micelle structure includes: The aqueous phase mixture is added dropwise to the oil phase mixture to form a viscous reverse micelle structure, with the viscosity controlled at 30000 Pa·s to 60000 Pa·s.

9. The application of the reverse micelle transporter according to claim 7 in the preparation of cosmetics that increase the intradermal retention of collagen, characterized in that, The process of mixing collagen with water to obtain an aqueous mixture includes: In the aqueous mixture, the collagen and water are mixed in a mass percentage ratio of (0.1% to 25%):(75% to 99.9%). Optionally, the process of mixing phospholipids, oils, and antioxidants to obtain an oil-phase mixture includes: The phospholipids, the oils and the antioxidants are mixed in a ratio of (15% to 60%):(53.55% to 80%):(0.5% to 1.5%) by weight percentage.

10. The application of the reverse micelle transporter according to claim 7 in the preparation of cosmetics that increase the intradermal retention of collagen, characterized in that, The step of adding the aqueous phase mixture dropwise to the oil phase mixture includes: By mass percentage, 0.1% to 2.0% of the aqueous phase mixture is added dropwise to 98% to 99.9% of the oil phase mixture, and the mixture is stirred.

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