Compositions and methods for enriching DNA using selection protection and a methylation-discriminating nuclease
By using a binding agent to protect recognition sequences during methylation-discriminating nuclease cleavage, the method enriches and retains partially-methylated and unmethylated molecules in DNA analysis, improving sensitivity and accuracy of cancer marker detection in liquid biopsies.
WO2026136756A1PCT designated stage Publication Date: 2026-06-25GUARDANT HEALTH INC
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- GUARDANT HEALTH INC
- Filing Date
- 2025-12-18
- Publication Date
- 2026-06-25
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Figure US2025060455_25062026_PF_FP_ABST
Abstract
The present disclosure provides compositions and methods related to enriching methylated DNA, such as cell-free DNA. In some embodiments, the cell-free DNA is from a subject having or suspected of having cancer and / or the cell-free DNA includes DNA from cancer cells. In some embodiments, the DNA is contacted with at least one binding agent that specifically binds a recognition sequence in the DNA, thereby providing sequence-specific bound DNA. In some embodiments, the sequence-specific bound DNA is contacted with at least one methylation-discriminating nuclease, thereby providing digested DNA. In some embodiments, the binding agent protects the recognition sequence from cleavage by a methylation-discriminating nuclease.
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