Soybean event cor-23134-4 and methods for detection thereof

Abstract

Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant soybean plant containing event COR-23134-4, and to assays for detecting the presence of event COR-23134-4 in samples and compositions thereof.

Description

CROSS REFERENCE

[0001] This application claims the benefit of U.S. Provisional Application No. 63 / 627235, filed on January 31, 2024, the disclosure of which is incorporated herein by reference in its entirety. REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY

[0002] An XML formatted sequence listing having the file name “ 108747-WO-SEC-l_SequenceListing.xml” created on January 14, 2025, and having a size of 1,569,000 bytes is filed in computer readable form concurrently with the specification. The sequence listing comprised in this XML formatted document is part of the specification and is herein incorporated by reference in its entirety. FIELD

[0003] Embodiments disclosed herein relate to the field of plant molecular biology, including to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein also include insect resistant soybean plants containing event COR-23134-4 and assays for detecting the presence of event COR-23134-4 in a sample and compositions thereof. BACKGROUND

[0004] Soybean is an important crop and is a primary food source in many areas of the world. Damage caused by insect pests is a major factor in the loss of the world’s soybean crops, despite the use of protective measures such as chemical pesticides. In view of this, insect resistance has been genetically engineered into crops such as soybean in order to control insect damage and to reduce the need for traditional chemical pesticides. One group of genes which have been utilized for the production of transgenic insect resistant crops is the delta-endotoxin group from Bacillus thuringiensis (Bt). Delta-endotoxins have been successfully expressed in crop plants such as cotton, potatoes, rice, sunflower, as well as corn, and in certain circumstances have proven to provide excellent control over insect pests. (Perlak, F.J et al. (1990) Bio / Technology 8:939-943; Perlak, F.J. etal. (1993) Plant Mol. Biol. 22:313-321; Fujimoto, H. etal. (1993) Bio / Technology 11:1151-1155; Tu etal. (2000)Nature Biotechnology 18:1101-1104; PCT publication WO 01 / 13731; and Bing, J.W. et al. (2000) Efficacy of CrylF Transgenic Maize, 14th Biennial International Plant Resistance to Insects Workshop, Fort Collins, CO). Soybean is a globally traded commodity produced in both temperate and tropical regions and serves as a key source of vegetable oils and protein. Soybean is grown as a commercial crop in over 35 countries. Brazil, United States, and Argentina together produce about 80% of the world’s soybean. Soybean is grown primarily to produce grain for further processing, has a multitude of uses in the food, feed, and industrial sectors, and represents one of the major sources of edible vegetable oil and of proteins for livestock feed use.

[0005] Certain lepidopteran insects are serious pests of soybean in various geographies. As adoption of Bt soybean has increased, the selection pressure on target insects to develop resistance has become greater. There remains a need for transgenic soybean plants that are resistant to certain insect pests in cultivation areas around the world. SUMMARY

[0006] The embodiments relate to the insect resistant soybean (Glycine max) plant event COR-23134-4, also referred to as “soybean line COR-23134-4,” “soybean event COR-23134-4,” and “COR-23134-4 soybean,” to the DNA plant expression construct of soybean plant event COR-23134-4, and to methods and compositions for the detection of the transgene construct, flanking, and insertion (the target locus) regions in soybean plant event COR-23134-4 and progeny thereof.

[0007] In one aspect compositions and methods relate to methods for producing and selecting an insect resistant dicot crop plant. Compositions include a DNA construct that when expressed in plant cells and plants confers resistance to insects. In one aspect, a DNA construct, capable of introduction into and replication in a host cell, is provided that when expressed in plant cells and plants confers insect resistance to the plant cells and plants. Soybean event COR-23134-4 was produced by transformation with plasmid PHP90315. As described herein, these events include the cry IB. 34.1 gene (polynucleotide SEQ ID NO: 4 and amino acid SEQ ID NO: 5) cassette, cry IB.61.1 gene (polynucleotide SEQ ID NO: 6 and amino acid SEQ ID NO: 7) cassette, and ipdO83Cb gene (polynucleotide SEQ ID NO: 8 and amino acid SEQ ID NO: 9) cassette (Table 1), each of which confers resistance to certain lepidopteran plant pests. The insect control components have demonstrated efficacy against lepidopteran insect species.

[0008] Some embodiments relate to specific flanking sequences of COR-23134-4 as described herein, which can be used to develop identification methods for COR-23134-4 in biological samples. More particularly, the disclosure relates to 5’ and / or 3’ flanking regions of COR-23134-4, which can be used for the development of specific primers and probes. Further embodiments relate to identification methods for the presence of COR-23134-4 in biological samples based on the use of such specific primers or probes.

[0009] According to some embodiments, methods of detecting the presence of DNA corresponding to the soybean event COR-23134-4 in a sample are provided. Such methods comprise: (a) contacting the sample comprising DNA with a DNA primer set, that when used in a nucleic acid amplification reaction with genomic DNA extracted from soybean comprising event COR-23134-4 produces an amplicon that is diagnostic for soybean event COR-23134-4, respectively; (b) performing a nucleic acid amplification reaction, thereby producing the amplicon; and (c) detecting the amplicon. In some aspects, the primer set comprises SEQ ID NOs: 18 and 19, and optionally a probe comprising SEQ ID NO: 20.

[0010] According to some embodiments, methods of detecting the presence of a DNA molecule corresponding to the COR-23134-4 event in a sample comprise: (a) contacting the sample comprising DNA extracted from a soybean plant with a DNA probe molecule that hybridizes under stringent hybridization conditions with DNA extracted from soybean containing event COR-23134-4 and does not hybridize under the stringent hybridization conditions with a control soybean plant DNA; (b) subjecting the sample and probe to stringent hybridization conditions; and (c) detecting hybridization of the probe to the DNA extracted from soybean containing event COR-23134-4. More specifically, a method for detecting the presence of a DNA molecule corresponding to the COR-23134-4 event in a sample comprises (a) contacting the sample comprising DNA extracted from a soybean plant with a DNA probe molecule that comprises sequences that are unique to the event, e.g. junction sequences, wherein said DNA probe molecule hybridizes under stringent hybridization conditions with DNA extracted from soybean event COR-23134-4 and does not hybridize under the stringent hybridization conditions with a control soybean plant DNA; (b) subjecting the sample and probe to stringent hybridization conditions; and (c) detecting hybridization of the probe to the DNA.

[0011] In addition, a kit and methods for identifying event COR-23134-4 in a biological sample which detects a COR-23134-4 specific region are provided.

[0012] DNA molecules are provided that comprise at least one junction sequence of COR-23134-4; wherein a junction sequence spans the junction located between heterologous DNA inserted into the genome and the DNA from the soybean cell flanking the insertion site. Detection of the junction sequence can be diagnostic for the COR-23134-4 event.

[0013] According to some embodiments, methods of producing an insect resistant soybean plant comprise the steps of: (a) sexually crossing a first parental soybean line comprising the expression cassettes disclosed herein, which confer resistance to insects, and a second parental soybean line that lacks such expression cassettes, thereby producing a plurality of progeny plants; and (b) selecting a progeny plant that is insect resistant. Such methods may optionally comprise the further step of back-crossing the progeny plant to the second parental soybean line to produce a true-breeding soybean plant that is insect resistant.

[0014] Some embodiments provide a method of producing a soybean plant that is resistant to insects comprising transforming a soybean cell with the DNA construct PHP90315, growing the transformed soybean cell into a soybean plant, selecting the soybean plant that shows resistance to insects, and further growing the soybean plant into a fertile soybean plant. The fertile soybean plant can be self-pollinated or crossed with compatible soybean varieties to produce insect resistant progeny. In some embodiments, a soybean plant comprises the genotype of the soybean event COR-23134-4, wherein said genotype comprises a nucleotide sequence as set forth in SEQ ID NO: 12 and SEQ ID NO: 15.

[0015] Some embodiments further relate to a DNA detection kit for identifying soybean event COR-23134-4 in biological samples. The kit comprises a first primer which specifically recognizes the 5’ or 3’ flanking region of COR-23134-4, and a second primer which specifically recognizes a sequence within the non-native target locus DNA of COR-23134-4, respectively, or within the flanking DNA, for use in a PCR identification protocol. A further embodiment relates to a kit for identifying event COR-23134-4 in biological samples, which kit comprises a specific probe having a sequence which corresponds or is complementary to, a sequence having between about 80% and 100% sequence identity with a specific region of event COR-23134-4. The sequence of the probe can correspond to a specific region comprising part of the 5’ or 3’ flanking region of event COR-23134-4. In some embodiments, the first or second primer comprises any one of SEQ ID NOs: 18, 19, 21, 22, 24, 25, 27, 28, 30, 31, 38, or 39.

[0016] The methods and kits encompassed by the embodiments disclosed herein can be used for different purposes such as, but not limited to the following: to identify event COR-23134-4 in plants, plant material or in products such as, but not limited to, food or feed products (fresh or processed) comprising, or derived from plant material; additionally or alternatively, the methods and kits can be used to identify transgenic plant material for purposes of segregation between transgenic and non-transgenic material; additionally or alternatively, the methods and kits can be used to determine the quality of plant material comprising soybean event COR-23134-4. The kits may also contain the reagents and materials necessary for the performance of the detection method.

[0017] A further embodiment relates to the COR-23134-4 soybean plant or its parts, including, but not limited to, pollen, ovules, vegetative cells, the nuclei of pollen cells, and the nuclei of egg cells of the soybean plant COR-23134-4 and the progeny derived thereof. In another embodiment, the DNA primer molecules targeting the soybean plant and seed of COR-23134-4 provide a specific amplicon product. DESCRIPTION OF THE DRAWINGS

[0018] FIG. 1. shows a schematic diagram of plasmid PHP90315 containing the crylB.34.1, crylB.61.1, ipdO83Cb, and gm-hra1 gene cassettes. Soybean event COR-23134-4 was created by Agrobacterium-mediated transformation with plasmid PHP90315. The size of plasmid PHP90315 is 35,909 bp. (SEQIDNO: 1).

[0019] FIG. 2. shows a schematic diagram of the PHP90315 T-DNA indicating the cry IB. 34.1, cry IB.61.1, ipdO83Cb, and gm-hra 1 gene cassettes. The size of the T-DNA is 27,801 bp (SEQIDNO: 2).

[0020] FIG. 3. shows a schematic Diagram of the Transformation and Development of COR-23134-4.

[0021] FIG. 4. Illustrates a recombinase-mediated excision.

[0022] FIG. 5. Illustrates a DNA DSB-mediated excision.

[0023] FIG. 6. Illustrates a type I-mediated sequence removal.

[0024] FIG. 7. Illustrates an example of cellular repair of DSB(s) by NHEJ, MMEJ, SSA, or HR can then be used to introduce targeted sequence variation in the promoter(s), intron(s), coding sequence(s) (CDS), terminator(s), and / or flanking sequence of COR-23134-4 to knockout or disrupt gene expression, up- or down-regulate gene expression, modify the coding content, and / or alter the flanking genomic polynucleotides.

[0025] FIG. 8. Illustrates an example of an RE, in which a target (for example but not limited to a loxP site) can be placed (either before or after genomic insertion) in opposing orientations and, then once recognized by the RE, the polynucleotide sequence between the sites inverted. DETAILED DESCRIPTION

[0026] As used herein the singular forms "a", "and", and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to "a cell" includes a plurality of such cells and reference to "the protein" includes reference to one or more proteins and equivalents thereof, and so forth. All technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs unless clearly indicated otherwise. Nucleic acid sequences listed in the accompanying sequence listing and referenced herein are shown using standard letter abbreviations for nucleotide bases. Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood to be included by any reference to the displayed strand.

[0027] Compositions of this disclosure include seed, deposited as NCMA Accession No.: 202305013, and plants, plant cells, and seed derived therefrom. Applicant s) deposited at least 625 seeds of soybean event COR-23134-4 (NCMA Accession No.: 202305013) with the Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA), 60 Bigelow Drive, East Boothbay, ME 04544 USA, on May 12, 2023. These deposits will be maintained under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure. The seeds deposited with the NCMA on May 12, 2023, were taken from the deposit maintained by Pioneer Hi-Bred International, Inc., 7250 NW 62nd Avenue, Johnston, Iowa 50131-1000. Access to this deposit will be available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. Upon allowance of any claims in the application, the Applicant(s) will make available to the public, pursuant to 37 C.F.R. § 1.808, sample(s) of the deposit of at least 625 seeds of hybrid soybean with the Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA). This deposit of seed of soybean event COR-23134-4 will be maintained in the NCMA depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicant(s) have satisfied all the requirements of 37 C.F.R. §§1.801 - 1.809, including providing an indication of the viability of the sample upon deposit. Applicant(s) have no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant(s) do not waive any infringement of their rights granted under this patent or rights applicable to event COR-23134-4 under the Plant Variety Protection Act (7 USC 2321 et seq.). Unauthorized seed multiplication is prohibited. The seed may be regulated.

[0028] A first gene cassette (crylB.34.1 gene cassette) contains the crylB.34.1 gene, a gene comprised of sequences from a crjlB-class gene and the crylCal gene, both derived from Bacillus thuringiensis (WO Patent 2016061197 [Izumi Wilcoxon and Yamamoto, 2016]; GenBank accession CAA30396.1, respectively). The expressed CrylB.34.1 protein confers control of certain susceptible lepidopteran pests. The CrylB.34.1 protein is 665 amino acids (aa) in length and has a molecular weight of approximately 75 kDa. Expression of the crylB.34.1 gene is controlled by the promoter region from the maize (Zea mays) histone H2B (zm-H2B) gene (GenBank accession NM_001196058.2; US Patent 6177611 [Rice, 2001]) and the 5' untranslated region (UTR) and intron region of the maize ubiquitin gene 1 (ubiZMl) (Christensen et al., 1992). The terminator for the crjlB.34.1 gene is the terminator region from the rice (Oryza sativa) ubiquitin (os-ubi) gene (WO Patent 2018102131 [Abbitt et al., 2018]). Two additional terminator regions from the sorghum (Sorghum bicolor) ubiquitin (sb-ubi) gene (Phytozome gene ID Sobic.004G049900.1; US Patent 9725731 [Abbitt, 2017]) and the sorghum actin (56-actin) gene (GenBank accession XM_002441128.2; US Patent 9725729 [Abbitt and Jung, 2017]) are present between the first and second gene cassettes. These additional terminators are intended to prevent any potential transcriptional interference. Transcriptional interference is defined as the transcriptional suppression of one gene on another when both are in proximity (Shearwin et al., 2005). The placement of one or multiple transcriptional terminators between gene cassettes has been shown to reduce the occurrence of transcriptional interference (Greger et al., 1998).

[0029] A second gene cassette (crjlB.61.1 gene cassette) contains the crjlB.61.1 gene, a modified cry\B-class gene, derived from Bacillus thuringiensis (WO Patent 2017180715 [Horn et al., 2017]). The expressed Cry IB.61.1 protein confers control of certain susceptible lepidopteran pests. The Cry IB.61.1 protein is 656 aa in length and has a molecular weight of approximately 74 kDa. Expression of the crjlB.61.1 gene is controlled by the promoter and the 5' UTR from the soybean chlorophyll a / b binding protein (gm-cab3) gene (Phytozome gene ID Glyma05g25810; US Patent 20200407742 [Sidorenko et al., 2020]). The os-T28 terminator (US Patent 10059953 [Bhyri et al., 2018]) for the crjlB.61.1 gene is the bidirectional terminator region from the rice NAC domain-containing protein gene (Phytozome gene ID LOC_Os03g60080.1) and the methylenetetrahydrofolate reductase gene (Phytozome gene ID LOC_Os03g60090.1). Two additional terminator regions from the sorghum phosphoenolpyruvic carboxylase (sb-PEPCl) gene (WO Patent 2018102131 [Abbitt et al., 2018]) and the Arabidopsis thaliana ubiquitin 3 (UBQ3) gene (WO Patent 2016149352 [Elsing et al., 2016] are present between the second and third gene cassettes to prevent possible transcriptional interference.

[0030] A third gene cassette (ipdO83Cb gene cassette) contains the insecticidal protein gene, ipdO83Cb, from giant maidenhair fern (Adiantum trapeziforme var. braziliense) (US Patent 10227608 [Barry et al., 2019]). The expressed IPD083Cb protein confers control of certain susceptible lepidopteran pests. The IPD083Cb protein is 853 aa in length and has a molecular weight of approximately 95 kDa. Expression of the ipdO83Cb gene is controlled by the promoter region from the common bean (Phaseolus vulgaris) ubiquitin 2 (pv-ubi2) gene including the 5' UTR and intron (Phytozome gene ID Phvul.003G123900.1). The os-T17 terminator (US Patent 10059953 [Bhyri et al., 2018]) for the ipdO83Cb gene is the bidirectional terminator region from the rice 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene (Phytozome gene ID LOC_Os06g04280.1) and the ribosomal protein S10 gene (Phytozome gene ID LOC_Os06g04290.1).

[0031] A fourth gene cassette (gm-hra 1 gene cassette) contains the gm-hra1 gene, a modified acetolactate synthase gene, from Glycine max (US Patent 7834242 [Falco and Li, 2010]). The expressed GM-HRA protein in plant tissue serves as a selectable marker during transformation which allows for the growth of tissue in the presence of sulfonylurea herbicides. The GM-HRA protein is 651 aa in length and has a molecular weight of approximately 70 kDa. Expression of the gm-hra_l gene is controlled by the promoter region from the soybean S-adenosyl-L-methionine synthetase (SAMS) gene including 5’ UTR and intron (US Patent 7834242 [Falco and Li, 2010]). The terminator for the gm-hra 1 gene is the terminator region from the soybean acetolactate synthase (als) gene (Phytozome gene ID Glyma.04G196100; US Patent 7834242 [Falco and Li, 2010]).

[0032] Additional terminator sequences are present adjacent to the Right Border and Left Border within the T-DNA region: the terminator region from the maize globulin-1 (zm-Glbl) gene (WO Patent 2014070646 [Albertsen et al., 2014]) and the terminator region from the maize 19-kDa zein (Z19) gene (GenBank accession KX247647.1; Dong et al., 2016), respectively.

[0033] The PHP90315 T-DNA contains one flippase recombinase target sequence, FRT1 (Proteau et al., 1986), and four attB recombination sites (Hartley et al., 2000; Katzen, 2007; Cheo et al., 2004). The presence of these sites alone does not cause any recombination, since to function, these sites need a specific recombinase enzyme that is not naturally present in plants (Cox, 1988; Dale and Ow, 1990; Thorpe and Smith, 1998).

[0034] According to some embodiments, compositions and methods are provided for identifying a novel soybean plant designated COR-23134-4 (NCMA Accession No.: 202305013). The methods are based on primers or probes which specifically recognize 5’ and / or 3’ flanking sequence of COR-23134-4. DNA molecules are provided that comprise primer sequences that when utilized in a PCR reaction will produce amplicons unique to the transgenic event COR-23134-4. In one embodiment, the soybean plant and seed comprising these molecules is contemplated. Further, kits utilizing these primer sequences for the identification of the COR-23134-4 event are provided.

[0035] As used herein, the term “soybean” means Glycine max or soybean and includes all plant varieties that can be bred with soybean, including wild soybean species.

[0036] As used herein, the terms “insect resistant” and “impacting insect pests” refers to effecting changes in insect feeding, growth, and / or behavior at any stage of development, including but not limited to: killing the insect; retarding growth; reducing reproductive capability; inhibiting feeding; and the like.

[0037] As used herein, the terms “pesticidal activity” and “insecticidal activity” are used synonymously to refer to activity of an organism or a substance (such as, for example, a protein) that can be measured by numerous parameters including, but not limited to, pest mortality, pest weight loss, pest attraction, pest repellency, and other behavioral and physical changes of a pest after feeding on and / or exposure to the organism or substance for an appropriate length of time. For example, “pesticidal proteins” are proteins that display pesticidal activity by themselves or in combination with other proteins.

[0038] As used herein, “insert DNA” refers to the heterologous DNA within the expression cassettes used to transform the plant material while “flanking DNA” can refer to either genomic DNA naturally present in an organism such as a plant, or foreign (heterologous) DNA introduced via the transformation process which is extraneous to the original insert DNA molecule, e.g., fragments associated with the transformation event. A “flanking region” or “flanking sequence” as used herein refers to a sequence of at least 10 bp (in some narrower embodiments, at least 20 bp, at least 50 bp, and up to at least 5000 bp), which is located either immediately upstream of and contiguous with and / or immediately downstream of and contiguous with the original non-native insert DNA molecule. Transformation procedures of the foreign DNA may result in transformants containing different flanking regions characteristic and unique for each transformant. When recombinant DNA is introduced into a plant through traditional crossing, its flanking regions will generally not be changed. It may be possible for single nucleotide changes to occur in the flanking regions through generations of plant breeding and traditional crossing. Transformants will also contain unique junctions between a piece of heterologous insert DNA and genomic DNA, or two (2) pieces of genomic DNA, or two (2) pieces of heterologous DNA. A "junction" is a point where two (2) specific DNA fragments join. For example, a junction exists where insert DNA joins flanking DNA. A junction point also exists in a transformed organism where two (2) DNA fragments join together in a manner that is modified from that found in the native organism. “Junction DNA” refers to DNA that comprises a junction point. Junction sequences set forth in this disclosure include a junction point located between the soybean genomic DNA and the 5’ end of the insert, which range from at least -5 to +5 nucleotides of the junction point (SEQ ID NO: 12), from at least -10 to +10 nucleotides of the junction point (SEQ ID NO: 13), and from at least -25 to +25 nucleotides of the junction point (SEQ ID NO: 14); and a junction point located between the 3’ end of the insert and soybean genomic DNA, which range from at least -5 to +5 nucleotides of the junction point (SEQ ID NO: 15), from at least -10 to +10 nucleotides of the junction point (SEQ ID NO: 16), and from at least -25 to +25 nucleotides of the junction point (SEQ ID NO: 17). Junction sequences set forth in this disclosure also include a junction point located between the target locus and the 5’ end of the insert. In some embodiments, SEQ ID NOs: 20 or 33 for COR-23134-4 represent the junction point located between the target locus and the 5’ end of the insert. The complete insert with flanking regions is represented in SEQ ID NO: 3.

[0039] In one embodiment, seeds, plants, and plant parts comprising soybean event COR-23134-4 are provided, wherein said seeds, plants, and plant parts comprise a DNA sequence chosen from SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, and SEQ ID NO: 17, or a DNA sequence chosen from a sequence having at least 95% sequence identity to SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, and SEQ ID NO: 17, wherein a representative sample of the soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013. In another embodiment, seeds, plants, and plant parts comprising soybean event COR-23134-4 are provided, wherein said seeds, plants, and plant parts comprise SEQ ID NO: 3 or a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 3, wherein a representative sample of the soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0040] As used herein, “heterologous” in reference to a nucleic acid sequence is a nucleic acid sequence that originates from a different non-sexually compatible species, or, if from the same species, is substantially modified from its native form in composition and / or genomic locus by deliberate human intervention. For example, a promoter operably linked to a heterologous nucleotide sequence can be from a species different from that from which the nucleotide sequence was derived, or, if from the same species, the promoter is not naturally found operably linked to the nucleotide sequence. A heterologous protein may originate from a foreign species, or, if from the same species, is substantially modified from its original form by deliberate human intervention.

[0041] The term “regulatory element” refers to a nucleic acid molecule having gene regulatory activity, i.e., one that has the ability to affect the transcriptional and / or translational expression pattern of an operably linked transcribable polynucleotide. The term “gene regulatory activity” thus refers to the ability to affect the expression of an operably linked transcribable polynucleotide molecule by affecting the transcription and / or translation of that operably linked transcribable polynucleotide molecule. Gene regulatory activity may be positive and / or negative and the effect may be characterized by its temporal, spatial, developmental, tissue, environmental, physiological, pathological, cell cycle, and / or chemically responsive qualities as well as by quantitative or qualitative indications.

[0042] “Promoter” refers to a nucleotide sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3' to a promoter sequence. The promoter sequence comprises proximal and more distal upstream elements, the latter elements are often referred to as enhancers. Accordingly, an “enhancer” is a nucleotide sequence that can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue-specificity of a promoter. Promoters may be derived in their entirety from a native gene or be composed of different elements derived from different promoters found in nature, or even comprise synthetic nucleotide segments. It is understood by those skilled in the art that different regulatory elements may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental conditions. Promoters that cause a nucleic acid fragment to be expressed in most cell types at most times are commonly referred to as “constitutive promoters”. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, nucleic acid fragments of different lengths may have identical or similar promoter activity.

[0043] The “translation leader sequence” refers to a nucleotide sequence located between the promoter sequence of a gene and the coding sequence. The translation leader sequence is present in the fully processed mRNA upstream of the translation start sequence. The translation leader sequence may affect numerous parameters including, processing of the primary transcript to mRNA, mRNA stability and / or translation efficiency.

[0044] The “3’ non-coding sequences” refer to nucleotide sequences located downstream of a coding sequence and include polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3 ’ end of the mRNA precursor.

[0045] A DNA construct is an assembly of DNA molecules linked together that provide one or more expression cassettes. The DNA construct may be a plasmid that is enabled for selfreplication in a bacterial cell and contains various endonuclease enzyme restriction sites that are useful for introducing DNA molecules that provide functional genetic elements, i.e., promoters, introns, leaders, coding sequences, 3’ termination regions, among others; or a DNA construct may be a linear assembly of DNA molecules, such as an expression cassette. The expression cassette contained within a DNA construct comprises the necessary genetic elements to provide transcription of a messenger RNA. The expression cassette can be designed to express in prokaryotic cells or eukaryotic cells. Expression cassettes of the embodiments are designed to express in plant cells.

[0046] The DNA molecules disclosed herein are provided in expression cassettes for expression in an organism of interest. The cassette includes 5’ and 3’ regulatory sequences operably linked to a coding sequence. “Operably linked” means that the nucleic acid sequences being linked are contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame. Operably linked is intended to indicate a functional linkage between a promoter and a second sequence, wherein the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence. The cassette may additionally contain at least one additional gene to be co-transformed into the organism. Alternatively, the additional gene(s) can be provided on multiple expression cassettes or multiple DNA constructs.

[0047] The expression cassette may include in the 5’ to 3’ direction of transcription: a transcriptional and translational initiation region, a coding region, and a transcriptional and translational termination region functional in the organism serving as a host. The transcriptional initiation region (e.g., the promoter) may be native or analogous, or foreign or heterologous to the host organism. Additionally, the promoter may be the natural sequence or alternatively a synthetic sequence. The expression cassettes may additionally contain 5’ leader sequences in the expression cassette construct. Such leader sequences can act to enhance translation.

[0048] It is to be understood that as used herein the term “transgenic” generally includes any cell, cell line, callus, tissue, plant part, or plant, the genotype of which has been altered by the presence of a heterologous nucleic acid including those initially so altered as well as those created by sexual crosses or asexual propagation from the initial transgenic and retains such heterologous nucleic acids.

[0049] A transgenic “event” is produced by transformation of plant cells with a heterologous DNA construct s), including a nucleic acid expression cassette that comprises a transgene of interest, the regeneration of a population of plants resulting from the insertion of the transgene into the genome of the plant, and selection of a particular plant characterized by insertion into a particular genome location. An event is characterized phenotypically by the expression of the transgene. At the genetic level, an event is part of the genetic makeup of a plant. The term “event” also refers to progeny produced by a sexual outcross between the transformant and another variety, wherein the progeny includes the heterologous DNA. After back-crossing to a recurrent parent, the inserted DNA and the linked flanking genomic DNA from the transformed parent is present in the progeny of the cross at the same chromosomal location. A progeny plant may contain sequence changes to the insert arising as a result of conventional breeding techniques. The term “event” also refers to DNA from the original transformant comprising the inserted DNA and flanking sequence immediately adjacent to the inserted DNA that would be expected to be transferred to a progeny that receives inserted DNA including the transgene of interest as the result of a sexual cross of one parental line that includes the inserted DNA (e.g., the original transformant and progeny resulting from selfing) and a parental line that does not contain the inserted DNA.

[0050] An insect resistant COR-23134-4 soybean plant may be bred by first sexually crossing a first parental soybean plant having the transgenic COR-23134-4 event plant and progeny thereof derived from transformation with the expression cassettes of the embodiments that confers insect resistance, and a second parental soybean plant that lacks such expression cassettes, thereby producing a plurality of first progeny plants; and then selecting a first progeny plant that is resistant to insects; and selfing the first progeny plant, thereby producing a plurality of second progeny plants; and then selecting from the second progeny plants an insect resistant plant. These steps can further include the back-crossing of the first insect resistant progeny plant or the second insect resistant progeny plant to the second parental soybean plant or a third parental soybean plant, thereby producing a soybean plant that is resistant to insects. The term “selfing” refers to self-pollination, including the union of gametes and / or nuclei from the same organism.

[0051] As used herein, the term "plant" includes reference to whole plants, parts of plants, plant organs (e.g., leaves, stems, roots, etc.), seeds, plant cells, and progeny of same. In some embodiments, parts of transgenic plants comprise, for example, plant cells, protoplasts, tissues, callus, embryos as well as flowers, stems, fruits, leaves, and roots originating in transgenic plants or their progeny previously transformed with a DNA molecule disclosed herein, and therefore consisting at least in part of transgenic cells.

[0052] As used herein, the term "plant cell" includes, without limitation, seeds, suspension cultures, embryos, meristematic regions, callus tissue, leaves, roots, shoots, gametophytes, sporophytes, pollen, and microspores. The class of plants that may be used is generally as broad as the class of higher plants amenable to transformation techniques, including both monocotyledonous and dicotyledonous plants.

[0053] “Transformation” refers to the transfer of a nucleic acid fragment into the genome of a host organism, resulting in genetically stable inheritance. Host plants containing the transformed nucleic acid fragments are referred to as “transgenic” plants.

[0054] As used herein, the term "progeny," in the context of event COR-23134-4, denotes an offspring of any generation of a parent plant which comprises soybean event COR-23134-4.

[0055] Isolated polynucleotides disclosed herein may be incorporated into recombinant constructs, typically DNA constructs, which are capable of introduction into and replication in a host cell. Such a construct may be a vector that includes a replication system and sequences that are capable of transcription and translation of a polypeptide-encoding sequence in a given host cell. A number of vectors suitable for stable transfection of plant cells or for the establishment of transgenic plants have been described in, e.g., Pouwels et al., (1985; Supp. 1987) Cloning Vectors: A Laboratory Manual, Weissbach and Weissbach (1989) Methods for Plant Molecular Biology, (Academic Press, New York); and Flevin et al., (1990) Plant Molecular Biology Manual, (Kluwer Academic Publishers). Typically, plant expression vectors include, for example, one or more cloned genes under the transcriptional control of 5’ and 3’ regulatory sequences and a dominant selectable marker. Such plant expression vectors also can contain a promoter regulatory region (e.g., a regulatory region controlling inducible or constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, a ribosome binding site, an RNA processing signal, a transcription termination site, and / or a polyadenylation signal.

[0056] During the process of introducing an insert into the genome of plant cells, it is not uncommon for some deletions or other alterations of the insert and / or genomic flanking sequences to occur. Thus, the relevant segment of the plasmid sequence provided herein might comprise some minor variations, including truncations. The same is possible for the flanking sequences and junction sequences provided herein. Thus, a plant comprising a polynucleotide having some range of identity with the subject flanking and / or insert sequences is within the scope of the subject disclosure. Identity to the sequence of the present disclosure may be a polynucleotide sequence having at least 65% sequence identity, at least 70% sequence identity, at least 75% sequence identity at least 80% identity, or at least 85% 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% sequence identity with a sequence exemplified or described herein. Hybridization and hybridization conditions as provided herein can also be used to define such plants and polynucleotide sequences of the subject disclosure. A sequence comprising the flanking sequences plus the full insert sequence can be confirmed with reference to the deposited seed.

[0057] In some embodiments, two different transgenic plants can also be crossed to produce offspring that contain two independently segregating added, exogenous genes. Selfing of appropriate progeny can produce plants that are homozygous for both added, exogenous genes. Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation.

[0058] A “probe” is an isolated nucleic acid to which is attached a conventional, synthetic detectable label or reporter molecule, e.g., a radioactive isotope, ligand, chemiluminescent agent, or enzyme. Such a probe is complementary to a strand of a target nucleic acid, for example, to a strand of isolated DNA from soybean event COR-23134-4 whether from a soybean plant or from a sample that includes DNA from the event. Probes may include not only deoxyribonucleic or ribonucleic acids but also polyamides and other modified nucleotides that bind specifically to a target DNA sequence and can be used to detect the presence of that target DNA sequence.

[0059] “Primers” are isolated nucleic acids that anneal to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, then extended along the target DNA strand by a polymerase, e.g., a DNA polymerase. Primer pairs refer to their use for amplification of a target nucleic acid sequence, e.g., by PCR or other conventional nucleic-acid amplification methods. “PCR” or “polymerase chain reaction” is a technique used for the amplification of specific DNA segments (see, U.S. Patent Nos. 4,683,195 and 4,800,159; herein incorporated by reference).

[0060] Probes and primers are of sufficient nucleotide length to bind to the target DNA sequence specifically in the hybridization conditions or reaction conditions determined by the operator. This length may be of any length that is of sufficient length to be useful in a detection method of choice. Generally, 11 nucleotides or more in length, 18 nucleotides or more, and 22 nucleotides or more, are used. Such probes and primers hybridize specifically to a target sequence under high stringency hybridization conditions. Probes and primers according to embodiments may have complete DNA sequence similarity of contiguous nucleotides with the target sequence, although probes differing from the target DNA sequence and that retain the ability to hybridize to target DNA sequences may be designed by conventional methods. Probes can be used as primers, but are generally designed to bind to the target DNA or RNA and are not used in an amplification process.

[0061] Specific primers may be used to amplify an integration fragment to produce an amplicon that can be used as a “specific probe” for identifying event COR-23134-4 in biological samples. When the probe is hybridized with the nucleic acids of a biological sample under conditions which allow for the binding of the probe to the sample, this binding can be detected and thus allow for an indication of the presence of event COR-23134-4 in the biological sample. In an embodiment of the disclosure, the specific probe is a sequence which, under appropriate conditions, hybridizes specifically to a region within the 5’ or 3’ flanking region of the event and also comprises a part of the foreign DNA contiguous therewith. The specific probe may comprise a sequence of at least 80%, from 80 and 85%, from 85 and 90%, from 90 and 95%, and from 95 and 100% identical (or complementary) to a specific region of the event.

[0062] Methods for preparing and using probes and primers are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 1989 (hereinafter, “Sambrook et al., 1989”); Ausubel et al. eds., Current Protocols in Molecular Biology,, Greene Publishing and Wiley-Interscience, New York, 1995 (with periodic updates) (hereinafter, “Ausubel et al., 1995”); and Innis et al., PCR Protocols: A Guide to Methods and Applications, Academic Press: San Diego, 1990. PCR primer pairs can be derived from a known sequence, for example, by using computer programs intended for that purpose such as the PCR primer analysis tool in Vector NTI version 6 (Informax Inc., Bethesda MD); PrimerSelect (DNASTAR Inc., Madison, WI); and Primer (Version 0.5©, 1991, Whitehead Institute for Biomedical Research, Cambridge, Mass.). Additionally, the sequence can be visually scanned and primers manually identified using guidelines known to one of skill in the art.

[0063] A “kit” as used herein refers to a set of reagents, and optionally instructions, for the purpose of performing method embodiments of the disclosure, more particularly, the identification of event COR-23134-4 in biological samples. A kit may be used, and its components can be specifically adjusted, for purposes of quality control (e.g., purity of seed lots), detection of event COR-23134-4 in plant material, or material comprising or derived from plant material, such as but not limited to food or feed products. “Plant material” as used herein refers to material which is obtained or derived from a plant.

[0064] Primers and probes based on the flanking DNA and insert sequences disclosed herein can be used to confirm (and, if necessary, to correct) the disclosed sequences by conventional methods, e.g., by re-cloning and sequencing such sequences. The nucleic acid probes and primers hybridize under stringent conditions to a target DNA sequence. Any conventional nucleic acid hybridization or amplification method may be used to identify the presence of DNA from a transgenic event in a sample.

[0065] A nucleic acid molecule is said to be the “complement” of another nucleic acid molecule if they exhibit complete complementarity or minimal complementarity. As used herein, molecules are said to exhibit “complete complementarity” when every nucleotide of one of the molecules is complementary to a nucleotide of the other. Two molecules are said to be “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional “low-stringency” conditions. Similarly, the molecules are said to be “complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional “high-stringency” conditions. Conventional stringency conditions are described by Sambrook et al., 1989, and by Haymes et al., In: Nucleic Acid Hybridization, a Practical Approach, IRL Press, Washington, D.C. (1985), departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure. In order for a nucleic acid molecule to serve as a primer or probe it need only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations employed.

[0066] In hybridization reactions, specificity is typically the function of post-hybridization washes, the critical factors being the ionic strength and temperature of the final wash solution. The thermal melting point (Tm) is the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridizes to a perfectly matched probe. For DNA-DNA hybrids, the Tm can be approximated from the equation of Meinkoth and Wahl (1984) Anal. Biochem. 138:267-284: Tm = 81.5 °C + 16.6 (logM) + 0.41 (%GC) - 0.61 (% form) - 500 / L; where M is the molarity of monovalent cations, %GC is the percentage of guanosine and cytosine nucleotides in the DNA, % form is the percentage of formamide in the hybridization solution, and L is the length of the hybrid in base pairs. Tm is reduced by about 1 °C for each 1% of mismatching; thus, Tm, hybridization, and / or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with >90% identity are sought, the Tm can be decreased 10 °C. Generally, stringent conditions are selected to be about 5 °C lower than the Tm for the specific sequence and its complement at a defined ionic strength and pH. However, in some embodiments, other stringency conditions can be applied, including severely stringent conditions can utilize a hybridization and / or wash at 1, 2, 3, or 4 °C lower than the Tm; moderately stringent conditions can utilize a hybridization and / or wash at 6, 7, 8, 9, or 10 °C lower than the Tm; low stringency conditions can utilize a hybridization and / or wash at 11, 12, 13, 14, 15, or 20 °C lower than the Tm.

[0067] Using the equation, hybridization and wash compositions, and desired Tm, those of ordinary skill will understand that variations in the stringency of hybridization and / or wash solutions are inherently described. If the desired degree of mismatching results in a Tm of less than 45 °C (aqueous solution) or 32 °C (formamide solution), a user may choose to increase the SSC concentration so that a higher temperature can be used. An extensive guide to the hybridization of nucleic acids is found in Tijssen (1993) Laboratory Techniques in Biochemistry and Molecular Biology—Hybridization with Nucleic Acid Probes, Part I, Chapter 2 (Elsevier, New York); and Ausubel et al., eds. (1995) and Sambrook et al. (1989).

[0068] In some embodiments, a complementary sequence has the same length as the nucleic acid molecule to which it hybridizes. In some embodiments, the complementary sequence is 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides longer or shorter than the nucleic acid molecule to which it hybridizes. In some embodiments, the complementary sequence is 1%, 2%, 3%, 4%, or 5% longer or shorter than the nucleic acid molecule to which it hybridizes. In some embodiments, a complementary sequence is complementary on a nucleotide-for-nucleotide basis, meaning that there are no mismatched nucleotides (each A pairs with a T and each G pairs with a C). In some embodiments, a complementary sequence comprises 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or less mismatches. In some embodiments, the complementary sequence comprises 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% or less mismatches.

[0069] "Percent (%) sequence identity" with respect to a reference sequence (subject) is determined as the percentage of amino acid residues or nucleotides in a candidate sequence (query) that are identical with the respective amino acid residues or nucleotides in the reference sequence, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity, and not considering any amino acid conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent sequence identity can be achieved in various ways that are within the skill in the art, for instance, using publicly available computer software such as BLAST, BLAST-2. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences (e.g., percent identity of query sequence = number of identical positions between query and subject sequences / total number of positions of query sequence *100).

[0070] Regarding the amplification of a target nucleic acid sequence (e.g., by PCR) using a particular amplification primer pair, stringent conditions permit the primer pair to hybridize only to the target nucleic-acid sequence to which a primer having the corresponding wild-type sequence (or its complement) would bind and optionally to produce a unique amplification product, the amplicon, in a DNA thermal amplification reaction.

[0071] As used herein, “amplified DNA” or “amplicon” refers to the product of nucleic acid amplification of a target nucleic acid sequence that is part of a nucleic acid template. For example, to determine whether a soybean plant resulting from a sexual cross contains transgenic event genomic DNA from the soybean plant disclosed herein, DNA extracted from a tissue sample of a soybean plant may be subjected to a nucleic acid amplification method using a DNA primer pair that includes a first primer derived from flanking sequence adjacent to the insertion site of inserted heterologous DNA, and a second primer derived from the inserted heterologous DNA to produce an amplicon that is diagnostic for the presence of the event DNA. Alternatively, the second primer may be derived from the flanking sequence. The amplicon is of a length and has a sequence that is also diagnostic for the event. The amplicon may range in length from the combined length of the primer pairs plus one nucleotide base pair to any length of amplicon producible by a DNA amplification protocol. Alternatively, primer pairs can be derived from flanking sequence on both sides of the inserted DNA so as to produce an amplicon that includes the entire insert nucleotide sequence of the PHP90315 expression construct as well as a portion of the sequence flanking the transgenic insert. A member of a primer pair derived from the flanking sequence may be located a distance from the inserted DNA sequence, this distance can range from one nucleotide base pair up to the limits of the amplification reaction. The use of the term “amplicon” specifically excludes primer dimers that may be formed in the DNA thermal amplification reaction.

[0072] Nucleic acid amplification can be accomplished by any of the various nucleic acid amplification methods known in the art, including PCR. A variety of amplification methods are known in the art and are described, inter alia, in U.S. Pat. Nos. 4,683,195 and 4,683,202 and in Innis et al., (1990) supra. PCR amplification methods have been developed to amplify up to 22 Kb of genomic DNA and up to 42 Kb of bacteriophage DNA (Cheng et al., Proc. Natl. Acad. Sci. USA 91:5695-5699, 1994). These methods as well as other methods known in the art of DNA amplification may be used in the practice of the embodiments of the present disclosure. It is understood that a number of parameters in a specific PCR protocol may need to be adjusted to specific laboratory conditions and may be slightly modified and yet allow for the collection of similar results. These adjustments will be apparent to a person skilled in the art.

[0073] The amplicon produced by these methods may be detected by a plurality of techniques, including, but not limited to, Genetic Bit Analysis (Nikiforov, etal. Nucleic Acid Res. 22:4167-4175, 1994) where a DNA oligonucleotide is designed which overlaps both the adjacent flanking DNA sequence and the inserted DNA sequence. The oligonucleotide is immobilized in wells of a microwell plate. Following PCR of the region of interest (for example, using one primer in the inserted sequence and one in the adjacent flanking sequence) a single-stranded PCR product can be hybridized to the immobilized oligonucleotide and serve as a template for a single base extension reaction using a DNA polymerase and labeled ddNTPs specific for the expected next base. Readout may be fluorescent or ELISA-based. A signal indicates presence of the insert / flanking sequence due to successful amplification, hybridization, and single base extension.

[0074] Another detection method is the pyrosequencing technique as described by Winge (2000) Innov. Pharma. Tech. 00:18-24. In this method an oligonucleotide is designed that overlaps the adjacent DNA and insert DNA junction. The oligonucleotide is hybridized to a single-stranded PCR product from the region of interest (for example, one primer in the inserted sequence and one in the flanking sequence) and incubated in the presence of a DNA polymerase, ATP, sulfurylase, luciferase, apyrase, adenosine 5’ phosphosulfate and luciferin. dNTPs are added individually and the incorporation results in a light signal which is measured. A light signal indicates the presence of the transgene insert / flanking sequence due to successful amplification, hybridization, and single or multi-base extension.

[0075] Fluorescence polarization as described by Chen et al., (1999) Genome Res. 9:492498 is also a method that can be used to detect an amplicon. Using this method an oligonucleotide is designed which overlaps the flanking and inserted DNA junction. The oligonucleotide is hybridized to a single-stranded PCR product from the region of interest (for example, one primer in the inserted DNA and one in the flanking DNA sequence) and incubated in the presence of a DNA polymerase and a fluorescent-labeled ddNTP. Single base extension results in incorporation of the ddNTP. Incorporation can be measured as a change in polarization using a fluorometer. A change in polarization indicates the presence of the transgene insert / flanking sequence due to successful amplification, hybridization, and single base extension.

[0076] Quantitative PCR (qPCR) is described as a method of detecting and quantifying the presence of a DNA sequence and is fully understood in the instructions provided by commercially available manufacturers. Briefly, in one such qPCR method, a FRET oligonucleotide probe is designed which overlaps the flanking and insert DNA junction. The FRET probe and PCR primers (one primer in the insert DNA sequence and one in the flanking genomic sequence) are cycled in the presence of a thermostable polymerase and dNTPs. Hybridization of the FRET probe results in cleavage and release of the fluorescent moiety away from the quenching moiety on the FRET probe. A fluorescent signal indicates the presence of the flanking / transgene insert sequence due to successful amplification and hybridization.

[0077] Molecular beacons have been described for use in sequence detection as described in Tyangi et al. (1996) Nature Biotech. 14:303-308. Briefly, a FRET oligonucleotide probe is designed that overlaps the flanking and insert DNA junction. The unique structure of the FRET probe results in it containing secondary structure that keeps the fluorescent and quenching moi eties in close proximity. The FRET probe and PCR primers (for example, one primer in the insert DNA sequence and one in the flanking sequence) are cycled in the presence of a thermostable polymerase and dNTPs. Following successful PCR amplification, hybridization of the FRET probe to the target sequence results in the removal of the probe secondary structure and spatial separation of the fluorescent and quenching moieties. A fluorescent signal results. A fluorescent signal indicates the presence of the flanking / transgene insert sequence due to successful amplification and hybridization.

[0078] A hybridization reaction using a probe specific to a sequence found within the amplicon is yet another method used to detect the amplicon produced by a PCR reaction.

[0079] Insect pests include insects selected from the orders Coleoptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthoptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, etc., particularly Lepidoptera.

[0080] Of interest are larvae and adults of the order Lepidoptera including, but not limited to, armyworms, cutworms, loopers and heliothines in the family Noctuidae, Spodoptera frugiperda JE Smith (fall army worm); S. exigua Hubner (beet army worm); S. litura Fabricius (tobacco cutworm, cluster caterpillar); Mamestra configurata Walker (bertha armyworm); M. brassicae Linnaeus (cabbage moth); Agrotis ipsilon Hufnagel (black cutworm); A. orthogonia Morrison (western cutworm); A. subterranea Fabricius (granulate cutworm); Alabama argillacea Hubner (cotton leaf worm); Trichoplusia ni Hubner (cabbage looper); Pseudoplusia includens Walker (soybean looper); Anticarsia gemmatalis Hubner (velvetbean caterpillar); Hypena scabra Fabricius (green cloverworm); Heliothis virescens Fabricius (tobacco budworm); Pseudaletia unipuncta Haworth (armyworm); Athetis mindara Barnes and Mcdunnough (rough skinned cutworm); Euxoa messoria Harris (darksided cutworm); Earias insulana Boisduval (spiny bollworm); E. vittella Fabricius (spotted bollworm); Helicoverpa armigera Hubner (American bollworm); H. zea Boddie (corn earworm or cotton bollworm); Melanchrapicta Harris (zebra caterpillar); Egira (Xylomyges) curialis Grote (citrus cutworm); borers, casebearers, webworms, coneworms, and skeletonizers from the family Pyralidae Ostrinia nubilalis Hubner (European corn borer); Amyelois transitella Walker (naval orangeworm); Anagasta kuehniella Zeller (Mediterranean flour moth); Cadra cautella Walker (almond moth); Chilo suppressalis Walker (rice stem borer); C. partellus, (sorghum borer); Corcyra cephalonica Stainton (rice moth); Crambus caliginosellus Clemens (corn root webworm); C. teterrellus Zincken (bluegrass webworm); Cnaphalocrocis medinalis Guenee (rice leaf roller); Desmia funeralis Hubner (grape leaffolder); Diaphania hyalinata Linnaeus (melon worm); D. nitidalis Stoll (pickleworm); Diatraea grandiosella Dyar (southwestern corn borer), D. saccharalis Fabricius (surgarcane borer); Eoreuma loftini Dyar (Mexican rice borer); Ephestia elutella Hubner (tobacco (cacao) moth); Galleria mellonella Linnaeus (greater wax moth); Herpetogramma licarsisalis Walker (sod webworm); Homoeosoma electellum Hulst (sunflower moth); Elasmopalpus lignosellus Zeller (lesser cornstalk borer); Achroia grisella Fabricius (lesser wax moth); Loxostege sticticalis Linnaeus (beet webworm); Orthaga thyrisalis Walker (tea tree web moth); Maruca testulalis Geyer (bean pod borer); Plodia interpunctella Hubner (Indian meal moth); Scirpophaga incertulas Walker (yellow stem borer); Udea rubigalis Guenee (celery leaftier); and leafrollers, budworms, seed worms and fruit worms in the family Tortricidae Acleris gloverana Walsingham (Western blackheaded budworm); A. variana Fernald (Eastern blackheaded budworm); Archips argyrospila Walker (fruit tree leaf roller); A. rosana Linnaeus (European leaf roller); and other Archips species, Adoxophyes orana Fischer von Rbsslerstamm (summer fruit tortrix moth); Cochylis hospes Walsingham (banded sunflower moth); Cydia latiferreana Walsingham (filbertworm); C. pomonella Linnaeus (coding moth); Platynota flavedana Clemens (variegated leafroller); P. stultana Walsingham (omnivorous leafroller); Lobesia botrana Denis & Schifferrmiller (European grape vine moth); Spilonota ocellana Denis & Schiffermuller (eyespotted bud moth); Endopiza viteana Clemens (grape berry moth); Eupoecilia ambiguella Hubner (vine moth); Bonagota salubricola Meyrick (Brazilian apple leafroller); Grapholita molesta Busck (oriental fruit moth); Suleima helianthana Riley (sunflower bud moth); Argyrotaenia spp.: and Choristoneura spp..

[0081] In one embodiment, of interest are larvae and adults of the order Lepidoptera including, but not limited to, Spodoptera frugiperda JE Smith (fall armyworm); S. exigua Hubner (beet army worm); S. litura Fabricius (tobacco cutworm, cluster caterpillar); Agrotis ipsilon Hufnagel (black cutworm); Trichoplusia ni Hubner (cabbage looper); Pseudoplusia includens Walker (soybean looper); Anticarsia gemmatalis Hubner (velvetbean caterpillar); Hypena scabra Fabricius (green cloverworm); Heliothis virescens Fabricius (tobacco budworm); Helicoverpa armigera Hubner (American bollworm); H. zea Boddie (corn earworm or cotton bollworm); and Pyralidae Ostrinia nubilalis Hubner (European corn borer).

[0082] In another embodiment, of interest are larvae and adults of the order Lepidoptera including, but not limited to, Spodoptera cosmioides Walker (garden army worm); Spodoptera albula Walker (gray-streaked armyworm); Spodoptera eridania Stoll (Southern armyworm); Chrysodeixis includens Walker; Rachiplusia nu Guenee (sunflower looper); Helicoverpa gelotopoeon Dyar (South American bollworm); Chloridea virescens Fabricius (this is current approved scientific name for tobacco budworm); Chrysodeixis acuta Walker (Tunbridge Wells Gem); and Thysanoplusia orichalcea Fabricius (slender burnished brass).

[0083] Selected other agronomic pests in the order Lepidoptera include, but are not limited to, Alsophilapometaria Harris (fall cankerworm); Anarsia lineatella Zeller (peach twig borer); Anisota senatoria J.E. Smith (orange striped oakworm); Antheraeapernyi Guerin-Meneville (Chinese Oak Tussah Moth); Bombyx mori Linnaeus (Silkworm); Bucculatrix thurberiella Busck (cotton leaf perforator); Colias eurytheme Boisduval (alfalfa caterpillar); Datana integerrima Grote & Robinson (walnut caterpillar); Dendrolimus sibiricus Tschetwerikov (Siberian silk moth), Ennomos subsignaria Hubner (elm spanworm); Erannis tiliaria Harris (linden looper); Euproctis chrysorrhoea Linnaeus (browntail moth); Harrisina americana Guerin-Meneville (grapeleaf skeletonizer); Hemileuca oliviae Cockrell (range caterpillar); Hyphantria cunea Drury (fall web worm); Keiferia lycopersicella Walsingham (tomato pinworm); Lambdinafiscellaria Hulst (Eastern hemlock looper); L. fiscellaria lugubrosa Hulst (Western hemlock looper); Leucoma salicis Linnaeus (satin moth); Lymantria dispar Linnaeus (gypsy moth); Manduca quinquemaculata Haworth (five spotted hawk moth, tomato hornworm); M. sexta Haworth (tomato homworm, tobacco homworm); Operophtera brumata Linnaeus (winter moth); Paleacrita vernata Peck (spring cankerworm); Papilio cresphontes Cramer (giant swallowtail orange dog); Phryganidia californica Packard (California oakworm); Phyllocnistis citrella Stainton (citrus leafminer); Phyllonorycter blancardella Fabricius (spotted tentiform leafminer); Pieris brassicae Linnaeus (large white butterfly); P. rapae Linnaeus (small white butterfly); P. napi Linnaeus (green veined white butterfly); Platyptilia carduidactyla Riley (artichoke plume moth); Plutella xylostella Linnaeus (diamondback moth); Pectinophora gossypiella Saunders (pink bollworm); Pontiaprotodice Boisduval and Leconte (Southern cabbageworm); Sabulodes aegrotata Guenee (omnivorous looper); Schizura concinna J.E. Smith (red humped caterpillar); Sitotroga cerealella Olivier (Angoumois grain moth); Thaumetopoeapityocampa Schiffermuller (pine processionary caterpillar); Tineola bisselliella Hummel (webbing clothesmoth); Tula absoluta Meyrick (tomato leafminer); Yponomeutapadella Linnaeus (ermine moth); Heliothis subflexa Guenee; Malacosoma spp. and Orgyia spp.

[0084] In some embodiments the COR-23134-4 soybean event may further comprise a stack of additional traits. Plants comprising stacks of polynucleotide sequences can be obtained by either or both of traditional breeding methods or through genetic engineering methods. These methods include, but are not limited to, breeding individual lines each comprising a polynucleotide of interest, transforming a transgenic plant comprising a gene disclosed herein with a subsequent gene and co- transformation of genes into a single plant cell. As used herein, the term “stacked” includes having the multiple traits present in the same plant (i.e., both traits are incorporated into the nuclear genome, one trait is incorporated into the nuclear genome and one trait is incorporated into the genome of a plastid or both traits are incorporated into the genome of a plastid).

[0085] In some embodiments the COR-23134-4 soybean event disclosed herein, alone or stacked with one or more additional insect resistance traits can be stacked with one or more additional input traits (e.g., herbicide resistance, fungal resistance, virus resistance, stress tolerance, disease resistance, male sterility, stalk strength, and the like) or output traits (e.g., increased yield, modified starches, improved oil profile, balanced amino acids, high lysine or methionine, increased digestibility, improved fiber quality, drought resistance, and the like). Thus, the embodiments can be used to provide a complete agronomic package of improved crop quality with the ability to flexibly and cost effectively control any number of agronomic pests.

[0086] In a further embodiment, the COR-23134-4 soybean event may be stacked with one or more additional insecticidal toxins, including, but not limited to, DAS814.19 (Conkesta soybean) (US Patent Number 8,680,363), MON87701 (US Patent Number 8,455,198); MON87751 (US Patent Number 9,719,145); In a further embodiment, the COR-23134-4 soybean event may be stacked with one or more additional transgenic events containing herbicide events, including, but not limited to, MON04032 (Roundup ready soybean); MON89788 (US Patent Number 7,632,985); MON87708 (U.S. Patent No. 9,447,428); A5547-127; LL2704; BPS-CV127-9 (U.S. Patent No. 9,024,114); MST-FG072-3; DP305423 (US Patent Number 9,816,098); DAS44406 (US Patent No: 9,540,655). Other such transgenic alleles conferring desirable traits may include herbicide tolerance: GTS 40-3-2, A2704-12, A2704-21, A5547-35, A5547-127, BPS-CV127-9 (U.S. Patent No. 9,024,114), DP356043 (U.S. Patent No. 7,951,995), GU262, W62, W98, DAS-68416-4 (US Patent No. 9,944,944), FG72, BPS-CV127-9, SYHT04R(U.S. Patent No. 10,006,044), SYHT0H2, 3560.4.3.5, EE-GM3, pDAB4472-1606, pDAB4468-0416, pDAB8291.45.36, 127, increased enhanced oil composition: DP-305423 (US Patent Number 8,609,935), G94-1, G94-19, G168, OT96-15, MON87705 (U.S. Patent No. 8,329,989), MON87769 (U.S. Patent No. 8,692,076); increased yield: MON 87712 (U.S. Patent No. 9,493,786), or nitrogen fixation traits, traits modulating the use of water, resistance to fungal infestation, resistance to nematode infestation, and the like. A non-transgenic property (e.g., QTL or maturity group) may also confer a desirable trait and one with skill in the art would know how to breed soybean to contain such non-transgenic trait and event COR-23134-4.

[0087] In a further embodiment, the COR-23134-4 soybean event may be stacked with one, two, three or more additional herbicidal tolerance traits enabling the selective use of multiple herbicide modes of action alone or in combinations. Glyphosate herbicide acts by inhibiting the EPSPS enzyme (5-enolpyruvylshikimate-3-phosphate synthase). This enzyme is involved in the biosynthesis of aromatic amino acids that are essential for growth and development of plants. A variety of glyphosate-insensitive EPSPS or glyphosate-metabolism enzymes are known. Genes that encode such enzymes can be operably linked to the gene regulatory elements of the subject disclosure. In an embodiment, such genes may include, but are not limited to, genes encoding glyphosate tolerance genes, which include: glyphosateinsensitive EPSPS genes such as 2mEPSPS, cp4 EPSPS, mEPSPS, dgt-28; epsps grg23ace5; and aroA genes; and as well as glyphosate-degradation genes such as glyphosate acetyl transferase genes (gat), and glyphosate oxidase genes (gox), for example. These traits may be found in products currently marketed as EnlistE3®, GT27, Gly-TolTM, Optimum® GAT®, Genuity Roundup Ready2 Yield®, and Roundup Ready®. Tolerance genes for glufosinate and / or bialaphos compounds include dsm-2, mat, hpat, bar and pat genes. The pat gene is in the trait currently marketed as LibertyLink®, LibertyLink GT27, EnlistE3®, ConkestaE3®, and Genuity Roundup Ready2 XtendFlex Soybean. Also included are tolerance genes that provide tolerance to 2,4-D (and other phenoxy auxin herbicides) such as aad-1, rdpA, and ft-t genes (it should be noted that these genes have further activity on aryl oxy phenoxy propionate (aka ‘fop’) herbicides) and aad-12, spdA, and tfdA genes (it should be noted that aad-12 genes have further activity on pyridyloxyacetate synthetic auxins like fluroxypyr and triclopyr). AAD-12 traits are marketed as Enlist® crop protection technology. Resistance genes for ALS inhibitors (sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinylthiobenzoates, and sulfonylamino-carbonyl-triazolinones) are known in the art. These resistance genes most commonly result from point mutations to the ALS encoding gene sequence. Other ALS inhibitor resistance genes include hra genes, the csrl-2 genes, surA genes, and surB genes. Some of the traits are marketed under the tradename Cultivance, STS, Bolt, or Optimum GAT. Herbicides that inhibit HPPD include the pyrazolones such as pyrazoxyfen, benzofenap, and topramezone; triketones such as mesotrione, sulcotrione, tembotrione, benzobicyclon; and diketonitriles such as isoxaflutole. These exemplary HPPD herbicides can be tolerated by known traits. Examples of HPPD inhibitors include hppdPF_W336 , hppdPf-4Pa , tdo / hisl / hsl 1, avhppd-03 genes (for resistance to isoxaflutole, meostrione, or other HPPD-inhibitor herbicides). An example of oxynil herbicide tolerant traits include the bxn gene, which has been showed to impart resistance to the herbicide / antibiotic bromoxynil. Resistance genes for dicamba include the dicamba monooxygenase gene (dmo) as disclosed in International PCT Publication No. WO 2008 / 105890. Resistance genes for PPO or PROTOX inhibitor type herbicides (e.g., acifluorfen, butafenacil, flupropazil, pentoxazone, carfentrazone, fluazolate, pyraflufen, aclonifen, azafenidin, flumioxazin, flumiclorac, bifenox, oxyfluorfen, lactofen, fomesafen, fluoroglycofen, epyrfenacil, saflufenacil, trifludimoxazin, tiafenacil, and sulfentrazone) are known in the art. Exemplary genes conferring resistance to PPO include herbicide insensitive forms of the PPO target enzyme from plants (US10100329, US10041087, US7671254, US6288306). Further exemplary genes conferring resistance to PPO include over expression of a wild-type Arabidopsis thaliana PPO enzyme (Lermontova I and Grimm B, (2000) Overexpression of plastidic protoporphyrinogen IX oxidase leads to resistance to the diphenylether herbicide acifluorfen. Plant Physiol 122:75-83.), the B. subtilis PPO gene (Li, X. and Nicholl D. 2005. Development of PPO inhibitor-resistant cultures and crops. Pest Manag. Sci. 61:277-285 and Choi KW, Han O, Lee HJ, Yun YC, Moon YH, Kim MK, Kuk YI, Han SU and Guh JO, (1998) Generation of resistance to the diphenyl ether herbicide, oxyfluorfen, via expression of the Bacillus subtilis protoporphyrinogen oxidase gene in transgenic tobacco plants. Biosci BiotechnolBiochem 62:558-560.) Resistance genes for ayrloxyphenoxypropionate(AOPP) and cyclohexone (DIM) include the herbicide insensitive forms of ACCase (AcetylCoA Carboxylase) genes (e.g., Accl-Sl, Accl-S2 and Accl-S3). Exemplary genes conferring resistance to cyclohexanediones and / or aryloxyphenoxypropanoic acid include sethoxydim, clethodim, cycloxydim, haloxyfop, diclofop, fenoxyprop, fluazifop, and quizalofop, among others. AOPP herbicide tolerance is also a second herbicide class enabled by genes such as aad-1, rdpA, andft t. Finally, herbicides can inhibit photosynthesis, including triazine or benzonitrile are provided tolerance by psbA genes (tolerance to triazine), Is genes (tolerance to triazine), and nitrilase genes (tolerance to benzonitrile). The above list of herbicide tolerance genes is not meant to be limiting. Any herbicide tolerance genes are encompassed by the present disclosure.

[0088] In some embodiments, the disclosed compositions can be introduced into the genome of a plant using genome editing technologies, or previously introduced polynucleotides in the genome of a plant may be edited using genome editing technologies. For example, the disclosed polynucleotides can be introduced into a desired location in the genome of a plant through the use of genome editing systems such as TALENs, meganucleases, zinc finger nucleases, CRISPR-Cas, and the like. For example, the disclosed polynucleotides can be introduced into a desired location in a genome using a CRISPR-Cas system, for the purpose of site-specific insertion. The desired location in a plant genome can be any desired target site for insertion, such as a genomic region amenable for breeding or may be a target site located in a genomic window with existing trait(s) of interest. Existing trait(s) of interest could be either endogenous traits or previously introduced traits.

[0089] In some embodiments, where the disclosed polynucleotide has previously been introduced into a genome, genome editing or genome engineering technologies may be used to alter or modify the introduced polynucleotide sequence, including flanking chromosomal genomic sequences. Site specific modifications that can be introduced into the disclosed compositions include those produced using any method for introducing site specific modification, including, but not limited to, through the use of sequence repair oligonucleotides, alone, or through the use of site-directed genome modification tools such as TALENs, meganucleases, zinc finger nucleases, CRISPR-Cas, and the like, with or without donor DNA. Site-specific modifications to the disclosed polynucleotides (including genomic flanking and junction sequences) may include, but are not limited to, changes to codon usage, changes to regulatory elements such as promoters, introns, terminators, enhancers, 5’ or 3’ untranslated regions (UTRs), or other noncoding sequences, and other regions of the polynucleotide, where the modifications do not adversely affect the phenotypic characteristics of the resulting soybean plant. COR-23134-4 event plants containing modified polynucleotide sequences are also contemplated herein.

[0090] Cas polypeptides suitable for introducing site-specific modifications include, for example, Cas9, Casl2f (Cas-alpha, Cas 14), Cas 121 (Cas-beta), Cas 12a (Cpfl), Cas 12b (a C2cl protein), Cas 13 (a C2c2 protein), Cas 12c (a C2c3 protein), Cas 12d, Casl2e, Cas 12g, Casl2h, Casl2i, Casl2j, Casl2k, Cas3, Cas3-HD, Cas 5, Cas6, Cas7, Cas8, CaslO, or combinations or complexes of these. In some aspects, transposon-associated TnpB, a programmable RNA-guided DNA endonuclease can be used.

[0091] In some aspects, a genome editing system comprises a Cas-alpha (e.g., Casl2f) endonuclease and one or more guide polynucleotides that introduce one or more site-specific modifications in a target polynucleotide sequence, resulting in a modified target sequence. As used herein, “altered target site”, “altered target sequence”, “modified target site,” and “modified target sequence” are used interchangeably and refer to a target sequence as disclosed herein that comprises at least one alteration or modification when compared to a non-altered target sequence. Such alterations or modifications include, for example: (i) replacement or substitution of at least one nucleotide, (ii) deletion of at least one nucleotide, (iii) insertion of at least one nucleotide, or (iv) any combination of (i) - (iii).

[0092] In some aspects, a genome editing system comprises a Cas-alpha endonuclease, one or more guide polynucleotides, and optionally a donor DNA. Some exemplary Cas-alpha endonucleases are described, for example, in WO2020123887.

[0093] In some aspects, a genome editing system comprises a Cas polypeptide, one or more guide polynucleotides, and optionally donor DNA, and editing a target polynucleotide sequence comprises nonhomologous end-joining (NHEJ) or homologous recombination (HR) following a Cas polypeptide-mediated double-strand break. Once a double-strand break is induced in the DNA, the cell's DNA repair mechanism is activated to repair the break. The most common repair mechanism to bring the broken ends together is the nonhomologous end-joining pathway (Bleuyard et al., (2006) DNA Repair 5:1-12). As a result, deletions, insertions, or other rearrangements are possible (Siebert and Puchta, (2002) Plant Cell 14:1121-31; Pacher et al., (2007) Genetics 175:21-9). Alternatively, the double-strand break can be repaired by homologous recombination between homologous DNA sequences. Once the sequence around the double-strand break is altered, for example, by exonuclease activities involved in the maturation of double-strand breaks, gene conversion pathways can restore the original structure if a homologous sequence is available, such as a homologous chromosome in non-dividing somatic cells, or a sister chromatid after DNA replication (Molinier et al., (2004) Plant Cell 16:342-52). Ectopic and / or epigenic DNA sequences may also serve as a DNA repair template for homologous recombination (Puchta, (1999) Genetics 152:1173-81).

[0094] In some aspects, the genome editing system comprises a Cas polypeptide, one or more guide polynucleotides, and a donor DNA. As used herein, “donor DNA” is a DNA construct that comprises a polynucleotide of interest to be inserted into the genomic target site of a Cas polypeptide. Once a double-strand break is introduced in the target site by the endonuclease, the first and second regions of homology of the donor DNA can undergo homologous recombination with their corresponding genomic regions of homology resulting in exchange of DNA between the donor DNA and the target genomic region. As such, the provided methods result in the integration of the polynucleotide of interest of the donor DNA into the double-strand break in the target site in the plant genome, thereby altering the original target site and producing an altered genomic target site.

[0095] In some aspects, a genome editing system comprises a base editing agent and a plurality of guide polynucleotides and editing a target polynucleotide sequence comprises introducing a plurality of nucleobase edits in the target polynucleotide sequence resulting in a variant nucleotide sequence. Other aspects include modified COR-23134-4 event plants produced using a genome editing system.

[0096] One or more nucleobases of a target genomic sequence can be chemically altered, in some cases to change the base from one type to another, for example from a Cytosine to a Thymine, or an Adenine to a Guanine. In some aspects, a plurality of bases, for example 2 or more, 5 or more, 10 or more, 20 or more, 30 or more, 40 or more, 50 or more, 60 or more, 70 or more, 80 or more 90 or more, 100 or more, or even greater than 100, 200 or more, up to thousands of bases may be modified or altered, to produce a plant with a plurality of modified bases.

[0097] Any base editing complex, such as a base editing agent associated with an RNA-guided polypeptide (such as e.g., dCas associated with a deaminase), may be used to target and bind to a desired locus in the genome of an organism and chemically modify one or more nucleotides of a target genomic sequence.

[0098] Site-specific nucleotide base conversions can be achieved to engineer one or more nucleotide changes to create one or more edits into the genome. These include for example, a site-specific base edit mediated by a C»G to T»A or an A»T to G»C base editing deaminase enzymes (Gaudelli et al., Programmable base editing of A»T to G»C in genomic DNA without DNA cleavage." Nature (2017); Nishida et al. “Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.” Science 353 (6305) (2016); Komor et al. “Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.” Nature 533 (7603) (2016):420-4. A catalytically “dead” or inactive Cas (dCas) polypeptide, for example an inactive Cas9 (dCas9), Casl2f (dCasl2f), or another Cas polypeptide disclosed herein, fused to a cytidine deaminase or an adenine deaminase protein becomes a specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->A on the opposite strand) or an adenine base editor that would convert adenine to inosine, resulting in an A->G change within an editing window specified by the guide polynucleotides. Any molecule that effects a change in a nucleobase is a “base editing agent”. The dCas forms a functional complex with a guide polynucleotide that shares homology with a genomic sequence at the target site, and is further complexed with the deaminase molecule. The guided Cas polypeptide recognizes and binds to a target sequence, opening the double-strand to expose individual bases. In the case of a cytidine deaminase, the deaminase deaminates the cytosine base and creates a uracil. Uracil glycosylase inhibitor (UGI) is provided to prevent the conversion of U back to C. DNA replication or repair mechanisms then convert the Uracil to a thymine (U to T), and subsequent repair of the opposing base (formerly G in the original G-C pair) to an Adenine, creating a T-A pair.

[0099] One or more nucleotides of the inserted event and / or the flanking genomic DNA can be modified using a prime editing technology. See e.g., Anzalone et al., Search-and-replace genome editing without double-strand breaks or donor DNA. Nature 576, 149-157 (2019). A prime editing complex includes a prime editing protein that contains an RNA-guided DNA-nicking domain, such as a Cas nickase (e.g., Cas9 nickase, Casdl2fl nickase), fused to a reverse transcriptase domain and complexed with a pegRNA. The PE-pegRNA complex is able to introduce targeted DNA edits at desired locations in the genome, by binding the target DNA and nicking the PAM-containing strand. The resulting 3' end hybridizes to a chosen primer binding site and then primes reverse transcription of new DNA sequence containing the desired edit using the reverse transcriptase template of the pegRNA. The resulting regulatory expression elements of the disclosed recombinant expression cassette(s) may be truncated or may include a polynucleotide sequence having at least 65% sequence identity, at least 70% sequence identity, at least 75% sequence identity at least 80% identity, or at least 85% 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% sequence identity with a regulatory element sequence exemplified or described herein.

[0100] Other modifications may include modifications to other portions of the DNA of the COR-23134-4 event. In some embodiments, genome engineering technologies can be used to relocate one or more expression cassettes described herein to one or more different locations of the same chromosome, or different chromosomes of soybean or a different crop. In such embodiment, polynucleotides comprising one or more of the junction sequences described herein (e.g., SEQ ID NOs: 12 and / or 15) may be retained with the expression cassette(s), either partially or fully, or may be removed. Furthermore, genomic flanking sequence(s) described herein may also be retained with the expression cassette(s), either partially or fully, or may be removed.

[0101] In another embodiment, genome engineering technologies may be used to co-locate one or more transgene(s) or expression cassette(s) in physical proximity to the 5’ or 3’ junction sequence(s) described herein. With regard to physical position on a chromosome, co-located transgenes and / or expression cassettes can be separated from the 5’ or 3’ junction sequence(s), e.g., by about 1 megabase (MB; 1 million nucleotides), about 500 kilobases (Kb; 1000 nucleotides), about 400 Kb, about 300 Kb, about 200 Kb, about 100 Kb, about 50 Kb, about 25 Kb, about 10 Kb, about 5 Kb, about 4 Kb, about 3 Kb, about 2 Kb, about 1 Kb, about 500 nucleotides, about 250 nucleotides, or less. With regard to genetic distance on a chromosome, co-located transgenes and / or expression cassettes can be separated from the 5’ or 3’ junction sequence(s), e.g., by about 10 cM, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.75, 0.5, 0.25, or 0.1 cM. For example, one or more of the expression cassette(s) obtained from one or more of the additional transgenic events described above may be co-located in physical proximity to the 5’ or 3’ junction sequence(s) described herein.

[0102] In another embodiment, polynucleotides comprising one of the junction sequences (e.g., SEQ ID NOs: 12 or 15) may be introduced at either or both ends of the inserted heterologous DNA. For example, a polynucleotide comprising the 5’ junction sequence may be deleted and replaced with a polynucleotide comprising the 3’ junction sequence, or vice versa.

[0103] In another embodiment, genome editing technologies may be used to modify the previously introduced polynucleotide(s) by inverting at least one of the polynucleotide(s) of the inserted DNA of the COR-23134-4 event. Such genome editing technologies can be used to modify the previously introduced polynucleotide through the insertion, deletion and / or substitution of one or more nucleotides within the introduced polynucleotide. Alternatively, double-stranded break technologies can be used to add additional nucleotide sequences to the introduced polynucleotide. Additional sequences that may be added include, but are not limited to, additional expression elements, such as enhancer and promoter sequences. Sequences that may be deleted include, but are not limited to, regulatory elements or portions thereof that when deleted do not adversely affect function. Modifications to modulate expression patterns (e.g., reducing the expression level of the insecticidal polypeptide in certain tissue) is also contemplated by site-directed modification to the introduced expression cassette.

[0104] In another embodiment, genome engineering technologies may be used to delete or modify all or part of one or more expression cassette(s) of the COR-23134-4 event as deposited with the NCMA on May 12, 2023, having NCMA Accession No.: 202305013. In this embodiment, the resulting soybean plant derived from the COR-23134-4 event as deposited with the NCMA on May 12, 2023, having NCMA Accession No.: 202305013 may comprise a portion of the expression cassette(s) described herein, none of the expression cassette(s) described herein, or modifications of the expression cassette(s) described herein.

[0105] In another embodiment, targeted DSB technologies may be used to position additional insecticidally-active proteins in close proximity to the disclosed compositions disclosed herein within the genome of a plant, in order to generate molecular stacks of insecticidally-active proteins.

[0106] In another embodiment, the polynucleotide sequences disclosed herein are used in a method comprising designing guide polynucleotides, such as guide RNAs (gRNAs), that recognize said polynucleotide sequences, synthesizing or obtaining said guide polynucleotides, and introducing said guide polynucleotides as part of genome engineering compositions to modify the DNA of the COR-23134-4 event as deposited with the NCMA on May 12, 2023, having NCMA Accession No.: 202305013. Such resulting modifications may include a polynucleotide sequence having at least 65% sequence identity, at least 70% sequence identity, at least 75% sequence identity at least 80% identity, or at least 85% 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% sequence identity with a sequence exemplified or described herein.

[0107] Embodiments include modified COR-23134-4 event plants produced using genome engineering technologies described herein.

[0108] One embodiment includes a soybean plant comprising the genotype of the soybean event COR-23134-4, wherein said genotype comprises a nucleotide sequence as set forth in SEQ ID NO: 12, 13, or 14, or SEQ ID NO: 15, 16, or 17 or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 12 or SEQ ID NO: 15.

[0109] Another embodiment includes a soybean plant comprising the genotype of the soybean event COR-23134-4, wherein said genotype comprises a nucleotide sequence as set forth in SEQ ID NO: 12, 13, or 14, and SEQ ID NO: 15, 16, or 17 or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 12, 13, or 14, and SEQ ID NO: 15, 16, or 17. Still another embodiment includes a soybean plant comprising the genotype of the soybean event COR-23134-4 or any prior embodiment, wherein said genotype comprises a nucleotide sequence having 1, 2, 3, 4, or 5 nucleotide changes in one or more of SEQ ID NO: 12-17 or SEQ ID NO: 3.

[0110] Another embodiment includes the soybean plant comprising the genotype of the soybean event COR-23134-4 of any prior embodiment, wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 13 and SEQ ID NO: 16, or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 13 and SEQ ID NO: 16.

[0111] Another embodiment includes the soybean plant comprising the genotype of the soybean event COR-23134-4 of any prior embodiment, wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 14 and SEQ ID NO: 17, or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 14 and SEQ ID NO: 17.

[0112] One embodiment includes a DNA construct comprising an operably linked first, second, and third expression cassettes.

[0113] In a further embodiment, the first expression cassette comprises: 1) a zm-H2B Promoter; 2) an ubiZMl Intron', 3) an cry IB. 34. T, and 4) an os-ubi Terminator.

[0114] In a further embodiment, the second expression cassette comprises: a) a gm-cab3 Promoter; b) a crylB.61.T, and c) an os-T28 Terminator.

[0115] In yet another embodiment, the third expression cassette comprises: i) a pv-ubi2 Promoter; ii) a pv-ubi2 Intron', iii) an ipdO83C; and iv) an os-Tl 7 Terminator.

[0116] Another embodiment includes a plant comprising the DNA construct comprising at least two operably linked expression cassette of any prior embodiment.

[0117] A further embodiment includes a plant comprising the DNA construct comprising at least two operably linked expression cassettes of any prior embodiment, wherein said plant is a soybean plant.

[0118] One embodiment includes a plant comprising the sequence set forth in SEQ ID NO: 33, or a sequence having at least 95% sequence identity to SEQ ID NO: 33.

[0119] One embodiment includes a soybean event COR-23134-4, wherein a representative sample of seed of said soybean event has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0120] Other embodiments include plant parts of the soybean event COR-23134-4 of any prior embodiments, wherein a representative sample of seed of said soybean event has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0121] One embodiment includes seed comprising soybean event COR-23134-4, wherein said seed comprises a DNA molecule chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein a representative sample of the soybean event COR-23134-4 seed of has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0122] Another embodiment includes a soybean plant, or part thereof, grown from the seed comprising soybean event COR-23134-4 of any prior embodiment, wherein said seed comprises a DNA molecule chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein a representative sample of the soybean event COR-23134-4 seed of has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0123] A further embodiment includes a transgenic seed produced from the soybean plant comprising soybean event COR-23134-4 of any prior embodiment, wherein a representative sample of the soybean event COR-23134-4 seed of has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0124] Other embodiments include a transgenic soybean plant, or part thereof, grown from the seed soybean produced from the soybean plant of soybean event COR-23134-4 of any prior embodiment, wherein a representative sample of the soybean event COR-23134-4 seed of has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0125] One embodiment includes an isolated nucleic acid molecule comprising a nucleotide sequence chosen from SEQ ID Nos: 12-17, 33-37, and 41, and full length complements thereof.

[0126] One embodiment includes an amplicon comprising the nucleic acid sequence chosen from SEQ ID NOs: 33-37, or 41 and full length complements thereof.

[0127] One embodiment includes a biological sample or extract derived from soybean event COR-23134-4 plant, tissue, or seed, wherein said sample or extract comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein said nucleotide sequence is detectable in said sample or extract using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013.

[0128] Another embodiment includes the biological sample or extract derived from soybean event COR-23134-4 plant, tissue, or seed, wherein said sample or extract comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein said nucleotide sequence is detectable in said sample or extract using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013 of any prior embodiment, wherein said biological sample comprises plant, plant tissue, or seed of transgenic soybean event COR-23134-4.

[0129] Another embodiment includes the biological sample or extract derived from soybean event COR-23134-4 plant, tissue, or seed, wherein said biological sample or extract comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein said nucleotide sequence is detectable in said sample or extract using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013 of any prior embodiment, wherein said biological sample or extract is a DNA sample extracted from the transgenic soybean plant event COR-23134-4, and wherein said DNA sample comprises one or more of the nucleotide sequences chosen from SEQ ID NOs: 12-17, 33-37, and 41, and the complement thereof.

[0130] Another embodiment includes the biological sample or extract derived from soybean event COR-23134-4 plant, tissue, or seed, wherein said biological sample or extract comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16 and SEQ ID NO: 17, wherein said nucleotide sequence is detectable in said sample or extract using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013 of any prior embodiment, wherein said biological sample or extract is chosen from soybean flour, soybean meal, and soybean oil manufactured in whole or in part to contain soybean byproducts.

[0131] One embodiment includes a method of producing hybrid soybean seeds comprising: a) sexually crossing a first inbred soybean line comprising a nucleotide chosen from SEQ ID NOs: 12-17, 33-37, and 41 and a second inbred line having a different genotype; b) growing progeny from said crossing; and c) harvesting the hybrid seed produced thereby.

[0132] Another embodiment includes the method of producing hybrid soybean seeds of any prior embodiment, wherein the first inbred soybean line is a female or a male parent.

[0133] One embodiment includes a method for producing a soybean plant resistant to lepidopteran pests comprising: a) sexually crossing a first parent soybean plant with a second parent soybean plant, wherein said first or second parent soybean plant comprises event COR-23134-4 thereby producing a plurality of first-generation progeny plants; b) selfing the first-generation progeny plant, thereby producing a plurality of second-generation progeny plants; and c) selecting from the second-generation progeny plants that comprise the event COR-23134-4 and are resistant to a lepidopteran pest.

[0134] Another embodiment includes a method of producing hybrid soybean seeds comprising: a) sexually crossing a first inbred soybean line comprising the DNA construct of claim 1 with a second inbred line not comprising the DNA construct of claim 1; and b) harvesting the hybrid seed produced thereby.

[0135] Another embodiment includes the method of producing a soybean plant resistant to lepidopteran pests of any prior embodiment, further comprising the step of backcrossing a second-generation progeny plant that comprises soybean event COR-23134-4 to the parent plant that lacks the soybean event COR-23134-4 DNA, thereby producing a backcross progeny plant that is resistant to a lepidopteran pest.

[0136] One embodiment includes a method of determining zygosity of a soybean plant comprising event COR-23134-4 in a biological sample comprising: a) contacting said sample with a first pair of DNA molecules and a second distinct pair of DNA molecules such that: 1) when used in a nucleic acid amplification reaction comprising soybean event COR-23134-4 DNA, produces a first amplicon that is diagnostic for event COR-23134-4, and 2) when used in a nucleic acid amplification reaction comprising soybean genomic DNA other than COR-23134-4 DNA, produces a second amplicon that is diagnostic for soybean genomic DNA other than COR-23134-4 DNA; b) performing a nucleic acid amplification reaction; and c) detecting the amplicons so produced, wherein detection of the presence of both amplicons indicates that said sample is heterozygous for soybean event COR-23134-4 DNA, wherein detection of only the first amplicon indicates that said sample is homozygous for soybean event COR-23134-4 DNA.

[0137] Another embodiment includes the method of determining zygosity of a soybean plant comprising event COR-23134-4 in a biological sample in any prior embodiment, wherein the first pair of DNA molecules comprises primer pair SEQ ID NOs: 18 and 19.

[0138] A further embodiment includes the method of determining zygosity of a soybean plant comprising event COR-23134-4 in a biological sample in any prior embodiment, wherein the first and second pair of DNA molecules comprise a detectable label.

[0139] Another embodiment includes the method of determining zygosity of a soybean plant comprising event COR-23134-4 in a biological sample in any prior embodiment, wherein the detectable label is a fluorescent label.

[0140] A further embodiment includes the method of determining zygosity of a soybean plant comprising event COR-23134-4 in a biological sample in any prior embodiment, wherein the detectable label is covalently associated with one or more of the primer molecules.

[0141] One embodiment includes a method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids, the method comprising: a) contacting the sample with a pair of primers that, when used in a nucleic-acid amplification reaction with genomic DNA from event COR-23134-4 produces an amplicon that is diagnostic for event COR-23134-4; b) performing a nucleic acid amplification reaction, thereby producing the amplicon that is diagnostic for event COR-23134-4; and c) detecting the amplicon that is diagnostic for event COR-23134-4.

[0142] Another embodiment includes the method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids of any prior embodiment, wherein the nucleic acid molecule that is diagnostic for event COR-23134-4 is an amplicon produced by the nucleic acid amplification chain reaction.

[0143] Another embodiment includes the method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids of any prior embodiment, wherein the method further comprises contacting the sample with a probe.

[0144] A further embodiment includes the method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids, further comprises contacting the sample with a probe of any prior embodiment, wherein the probe comprises a detectable label.

[0145] A further embodiment includes the method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids further comprising contacting the sample with a probe, wherein the probe comprises a detectable label of any prior embodiment, wherein the detectable label is a fluorescent label.

[0146] A further embodiment includes the method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids further comprising contacting the sample with a probe, wherein the probe comprises a

[0147] detectable label of any prior embodiment, wherein the detectable label is covalently associated with the probe.

[0148] One embodiment includes a plurality of polynucleotide primers comprising one or more polynucleotides which target event COR-23134-4 DNA template in a sample to produce an amplicon diagnostic for event COR-23134-4 as a result of a polymerase chain reaction method.

[0149] Another embodiment includes a plurality of polynucleotide primers according to any prior embodiment, wherein a) a first polynucleotide primer comprises a nucleotide sequence as set forth in SEQ ID NO: 18, and the complements thereof; and b) a second polynucleotide primer comprises a nucleotide sequence as set forth in SEQ ID NO: 19, and the complements thereof.

[0150] Another embodiment includes the primers of any prior embodiment, wherein said first primer and said second primer are at least 19 nucleotides.

[0151] One embodiment includes a method of detecting the presence of DNA corresponding to event COR-23134-4 in a sample, the method comprising: a) contacting the sample comprising soybean DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from soybean event COR-23134-4 and does not hybridize under said stringent hybridization conditions with a non- COR-23134-4 soybean plant DNA; b) subjecting the sample and probe to stringent hybridization conditions; and c) detecting hybridization of the probe to the DNA; wherein detection of hybridization indicates the presence of event COR-23134-4.

[0152] One embodiment includes a kit for detecting nucleic acids that are unique to event COR-23134-4 comprising at least one nucleic acid molecule of sufficient length of contiguous polynucleotides to function as a primer or probe in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event COR-23134-4 in the sample.

[0153] Another embodiment includes the kit for detecting nucleic acids that are unique to event COR-23134-4 comprising at least one nucleic acid molecule of sufficient length of contiguous polynucleotides to function as a primer or probe in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event COR-23134-4 in the sample of any prior embodiment, wherein the nucleic acid molecule comprises a nucleotide sequence from SEQ ID NO: 12-41.

[0154] Another embodiment includes the kit for detecting nucleic acids that are unique to event COR-23134-4 comprising at least one nucleic acid molecule of sufficient length of contiguous polynucleotides to function as a primer or probe in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event COR-23134-4 in the sample of any prior embodiment, wherein the nucleic acid molecule is a primer chosen from SEQ ID NOs: 12-41, and the complements thereof.

[0155] Another embodiment includes the soybean plant comprising the genotype of the soybean event COR-23134-4 of any prior embodiment, wherein the genotype comprises a nucleotide sequence having 1, 2, 3, 4, or 5 nucleotide changes in one or more of SEQ ID Nos: 12-17, or SEQIDNO:3.

[0156] Another embodiment includes the com plant comprising the genotype of the soybean event COR-23134-4 of any prior embodiment, further comprising the nucleotide sequence set forth in SEQ ID NO: 3, or a nucleotide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the nucleotide sequence of SEQ ID NO: 3. In yet another embodiment, the corn plant comprising the genotype of the soybean event COR-23134-4 of any prior embodiment, further comprising the nucleotide sequence set forth in SEQ ID NO: 3, or a nucleotide sequence having 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotide changes to the nucleotide sequence of SEQ ID NO: 3.

[0157] One embodiment includes a method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event.

[0158] Another embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event of any prior embodiment, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO: 3. In yet another embodiment, the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event 40 of any prior embodiment, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 nucleotide changes to the nucleotide sequence of SEQ ID NO: 3.

[0159] Another embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event of any prior embodiment, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having all or a portion of SEQ ID NO: 12 or SEQ ID NO: 15 duplicated in said modified DNA sequence.

[0160] Another embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event of any prior embodiment, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3.

[0161] A further embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3 of any prior embodiment, wherein said excision comprises an excision from one or more regulatory elements of SEQ ID NO: 3 that does not substantially affect the activity of said one or more regulatory elements.

[0162] A further embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3 of any prior embodiment, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having all or a portion of SEQ ID NO: 12 or SEQ ID NO: 15 excised from said modified DNA sequence.

[0163] Another embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3 of any prior embodiment, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having at least 30% of SEQ ID NO: 3 excised from said modified DNA sequence.

[0164] A further embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3 of any prior embodiment, wherein at least 80% of SEQ ID NO: 3 is excised from said modified DNA sequence.

[0165] Another embodiment includes the method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3 of any prior embodiment, wherein all of SEQ ID NO: 3 is excised from said modified DNA sequence.

[0166] One embodiment includes a method of generating guide polynucleotides for use with a COR-23134-4 soybean event genome editing system comprising designing one or more guide polynucleotides that recognize at least a portion of SEQ ID NO: 3 and synthesizing said guide polynucleotides.

[0167] Another embodiment includes a method of modifying the DNA of the COR-23134-4 event having NCMA Accession No.: 202305013 comprising introducing said one or more guide polynucleotides for use with a COR-23134-4 soybean event genome editing system of any prior embodiment as part of a genome engineering composition to a DNA of the COR-23134-4 event to modify the DNA of the COR-23134-4 event.

[0168] One embodiment includes a COR-23134-4 soybean event genome editing system comprising a CAS polypeptide, one or more guide polynucleotides, and COR-23134-4 soybean event donor DNA.

[0169] One embodiment includes a method of modifying at least one expression cassette of the COR-23134-4 event as deposited with the NCMA having NCMA Accession No.: 202305013, wherein the method comprises using genome editing technologies to modify at least one expression cassette, wherein the resulting soybean plant derived from the COR-23134-4 event comprises at least one modified cassette.

[0170] Another embodiment includes the method of modifying at least one expression cassette of the COR-23134-4 event as deposited with the NCMA having NCMA Accession No.: 202305013, wherein the method comprises using genome editing technologies to modify at least one expression cassette, wherein the resulting soybean plant derived from the COR-23134-4 event comprises at least one modified cassette of any prior embodiment, wherein the method comprises altering expression of crylB.34.1.

[0171] Another embodiment includes the method of modifying at least one expression cassette of the COR-23134-4 event as deposited with the NCMA having NCMA Accession No.: 202305013, wherein the method comprises using genome editing technologies to modify at least one expression cassette, wherein the resulting soybean plant derived from the COR-23134-4 event comprises at least one modified cassette of any prior embodiment, wherein the method comprises altering expression of crylB.61.1.

[0172] Another embodiment includes the method of modifying at least one expression cassette of the COR-23134-4 event as deposited with the NCMA having NCMA Accession No.: 202305013, wherein the method comprises using genome editing technologies to modify at least one expression cassette, wherein the resulting soybean plant derived from the COR-23134-4 event comprises at least one modified cassette of any prior embodiment, wherein the method comprises altering expression of IPD083Cb.

[0173] One embodiment includes a method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4.

[0174] Another embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4 of any prior embodiment, wherein the Lepidopteran insect is Fall Armyworm.

[0175] Another embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4 of any prior embodiment, wherein the Lepidopteran insect is Soybean Looper.

[0176] Another embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4 of any prior embodiment, wherein the Lepidopteran insect is Velvetbean Caterpillar.

[0177] Another embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4 of any prior embodiment, wherein the damage from the Lepidopteran insect is controlled for soybean event COR-23134-4.

[0178] Another embodiment includes a method of producing a commodity plant product comprising processing grain produced from a soybean event COR-23134-4 plant comprising a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with Provasoli-Guillard National Center for Marine Algae and Microbiota (NCMA) with NCMA Accession No.: 202305013, wherein said grain is processed into a commodity plant product chosen from soybean flour, soybean meal, and soybean oil manufactured in whole or in part to contain soybean by-products, wherein said composition / commodity plant product comprises a detectable amount of said nucleotide sequence.

[0179] One embodiment includes a method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4.

[0180] Another embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4, wherein the Lepidopteran insect is Fall Armyworm (Spodoptera frugiperda).

[0181] Yet another embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4, wherein the Lepidopteran insect is Fall Armyworm (Spodoptera frugiperda). Soybean Looper (Chrysodeixis includens), or Velvetbean Caterpillar (Anticarsia gemmatalis).

[0182] A further embodiment includes the method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4, wherein damage from the Lepidopteran insect is controlled for soybean pods or seeds from event COR-23134-4.

[0183] In some embodiments, a soybean plant comprising a COR-23134-4 event may be treated with a seed treatment. In some embodiments, the seed treatment may be a fungicide, an insecticide, or a herbicide.

[0184] The following examples are offered by way of illustration and not by way of limitation. EXAMPLES

[0185] The following Examples are included to more fully describe embodiments of the development of soy event COR-23134-4, which resulted from the construction of 19 different construct designs having IPD083Cb, Cry IB.61.1, and CrylB.34.1. About 4,750 TO events were generated, of which 69 events were tested in T1 for homozygous plants, of which 57 events were tested in field trials. Eighteen events were tested in up to four different genotypes. Of these, 8 events were tested in seed field trials and soy event COR-23134-4 was subsequently selected. These field tests were conducted over the course of 5 years. Example 1. Cassette Design -

[0186] Soybean (Glycine max [L.] Merr) was created by ^grotocterzwm-mediated transformation with plasmid PHP90315 (Figure 1). The T-DNA region is represented schematically in Figure 2. Summary of the genetic elements and their positions on the T-DNA are provided in Table 1.

[0187] The T-DNA of plasmid PHP90315 contains four gene cassettes. The first gene cassette (cry IB. 34.1 gene cassette) contains the cry IB. 34.1 gene, a gene composed of sequences from a crj / B-class gene and the crylCal gene, both derived from Bacillus thuringiensis (WO Patent 2016061197 [Izumi Wilcoxon and Yamamoto, 2016]; GenBank accession CAA30396.1, respectively). The expressed Cry IB.34.1 protein confers control of certain susceptible lepidopteran pests. The Cry IB.34.1 protein is 665 amino acids (aa) in length and has a molecular weight of approximately 75 kDa. Expression of the crylB.34.1 gene is controlled by the promoter region from the maize (Zea mays') histone H2B (zm-H2B) gene (GenBank accession NM_001196058.2; US Patent 6177611 [Rice, 2001]) and the 5' untranslated region (UTR) and intron region of the maize ubiquitin gene 1 (ubiZMl) (Christensen et al., 1992). The terminator for the crylB.34.1 gene is the terminator region from the rice (Oryza sativa) ubiquitin (os-ubi) gene (WO Patent 2018102131 [Abbitt et al., 2018]). Two additional terminator regions from the sorghum (Sorghum bicolor) ubiquitin (sb-ubi) gene (Phytozome gene ID Sobic.004G049900.1; US Patent 9725731 [Abbitt, 2017]) and the sorghum actin (s / >-actin) gene (GenBank accession XM 002441128.2; US Patent 9725729 [Abbitt and Jung, 2017]) are present between the first and second gene cassettes. These additional terminators are intended to prevent any potential transcriptional interference. Transcriptional interference is defined as the transcriptional suppression of one 46 gene on another when both are in proximity (Shearwin et al., 2005). The placement of one or multiple transcriptional terminators between gene cassettes has been shown to reduce the occurrence of transcriptional interference (Greger et al., 1998).

[0188] The second gene cassette (cry IB.61.1 gene cassette) contains the cry IB.61.1 gene, a modified crj / B-class gene, derived from Bacillus thuringiensis (WO Patent 2017180715 [Hom et al., 2017]). The expressed Cry IB.61.1 protein confers control of certain susceptible lepidopteran pests. The Cry IB.61.1 protein is 656 aa in length and has a molecular weight of approximately 74 kDa. Expression of the crjlB.61.1 gene is controlled by the promoter and the 5' UTR from the soybean chlorophyll a / b binding protein (gincabs') gene (Phytozome gene ID Glyma05g25810; US Patent 20200407742 [Sidorenko et al., 2020]). The os-T28 terminator (US Patent 10059953 [Bhyri et al., 2018]) for the crjlB.61.1 gene is the bidirectional terminator region from the rice NAC domaincontaining protein gene (Phytozome gene ID LOC_Os03g60080.1) and the methylenetetrahydrofolate reductase gene (Phytozome gene ID LOC_Os03g60090.1). Two additional terminator regions from the sorghum phosphoenolpyruvic carboxylase (sb-PEPCB) gene (WO Patent 2018102131 [Abbitt et al., 2018]) and the Arabidopsis thaliana ubiquitin 3 (UBQ3) gene (WO Patent 2016149352 [Elsing et al., 2016] are present between the second and third gene cassettes to prevent possible transcriptional interference.

[0189] The third gene cassette (ipdO83Cb gene cassette) contains the insecticidal protein gene, ipdO83Cb. from giant maidenhair fern (Adiantum trapeziforme var. braziliense) (US Patent 10227608 [Barry et al., 2019]). The expressed IPD083Cb protein confers control of certain susceptible lepidopteran pests. The IPD083Cb protein is 853 aa in length and has a molecular weight of approximately 95 kDa. Expression of the ipdO83Cb gene is controlled by the promoter region from the common bean (Phaseolus vulgaris) ubiquitin 2 (pv-ubi2) gene including the 5' UTR and intron (Phytozome gene ID Phvul.003G123900.1). The os-T17 terminator (US Patent 10059953 [Bhyri et al., 2018]) for the ipdO83Cb gene is the bidirectional terminator region from the rice 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene (Phytozome gene ID LOC_Os06g04280.1) and the ribosomal protein S10 gene (Phytozome gene ID LOC_Os06g04290.1).

[0190] The fourth gene cassette (gm-hra1 gene cassette) contains the gm-hra1 gene, a modified acetolactate synthase gene, from Glycine max (US Patent 7834242 [Falco and Li, 2010]). The expressed GM-HRA protein in plant tissue serves as a selectable marker during transformation which allows for the growth of tissue in the presence of sulfonylurea herbicides. The GM-HRA protein is 651 aa in length and has a molecular weight of approximately 70 kDa. Expression of the gm-hra_l gene is controlled by the promoter region from the soybean S-adenosyl-L-methionine synthetase (SAMS) gene including 5’ UTR and intron (US Patent 7834242 [Falco and Li, 2010]). The terminator for the gm-hra 1 gene is the terminator region from the soybean acetolactate synthase (ah) gene (Phytozome gene ID Glyma.04G196100; US Patent 7834242 [Falco and Li, 2010]).

[0191] Additional terminator sequences are present adj acent to the Right Border and Left Border within the T-DNA region: the terminator region from the maize globulin-1 (zm-G / hi) gene (WO Patent 2014070646 [Albertsen et al., 2014]) and the terminator region from the maize 19-kDa zein (Z19) gene (GenBank accession KX247647.1; Dong et al., 2016), respectively.

[0192] The PHP90315 T-DNA contains one flippase recombinase target sequence, FRT1 (Proteau et al., 1986), and four attB recombination sites (Hartley et al., 2000; Katzen, 2007; Cheo et al., 2004). The presence of these sites alone does not cause any recombination, since to function, these sites need a specific recombinase enzyme that is not naturally present in plants (Cox, 1988; Dale and Ow, 1990; Thorpe and Smith, 1998). Table 1: Description of Genetic Elements in the T-DNA Region from Plasmid PHP90315 Gene Cassette Location on T-DNA (bp to bp) Genetic Element Size (bp) Description 1-25 Right Border (RB) 25 T-DNA Right Border from the Agrobacterium tumefaciens Ti plasmid (GenBank accession KX986282.1; Komari et al., 1996) 26-48 Ti Plasmid Region 23 Sequence from the Agrobacterium tumefaciens Ti plasmid (GenBank accession KX986282.1; Komari et al., 1996) 49-89 Intervening Sequence 41 DNA sequence used for cloning 90- 1,083 zm-Glbl Terminator 994 Terminator region from the Zea mays globulin-1 gene (WO Patent 2014070646 [Albertsen et al., 2014]) 1,084 1,324 Intervening Sequence 241 DNA sequence used for cloning 1,325 1,345 a#B4 21 Bacteriophage lambda integrase recombination site (Cheo et al., 2004) 1,346 1,394 Intervening Sequence 49 DNA sequence used for cloning crjlB.34.1 gene cassette 1,395 2,344 (complement ary) os-ubi Terminator 950 Terminator region from the Oryza sativa (rice) ubiquitin gene (WO Patent 2018102131 [Abbitt et al., 2018]) 2,345 -2,350 Intervening Sequence 6 DNA sequence used for cloning 2,351 -4,348 (complement ary) cry IB. 34.1 1,998 Gene composed of sequences from a crjlB-class gene and the crylCal1 gene, both derived from Bacillus thuringiensis (WO Patent 2016061197 [Izumi Wilcoxon and Yamamoto, 2016]; GenBank accession CAA30396.1, respectively) as described below: crjlB-class at bp 4,348 - 2,879 (1,470 bp long) crylCal at bp 2,878 - 2,351 (528 bp long) 4,349 4,377 Intervening Sequence 29 DNA sequence used for cloning 4,3785,390 (complement ary) ubiZMl Intron 1,013 Intron region from the Zea mays ubiquitin gene 1 (Christensen et al., 1992) 5,391 -5,472 (complement ary) ubiZMl 5' UTR 82 5' untranslated region from the Zea mays ubiquitin gene 1 (Christensen et al., 1992) 5,473 -5,496 Intervening Sequence 24 DNA sequence used for cloning 5,4976,948 (complement ary) zm-H2B Promoter 1,452 Promoter region from the Zea mays histone H2B gene (GenBank accession NM_001196058.2; US Patent 6177611 [Rice, 2001]) 6,949 -6,989 Intervening Sequence 41 DNA sequence used for cloning 6,990 -7,013 a / / BI 24 Bacteriophage lambda integrase recombination site from the Invitrogen Gateway® Cloning system (Hartley et al., 2000; Katzen, 2007) 7,014 7,194 Intervening Sequence 181 DNA sequence used for cloning 7,1957,778 (complement ary) sb-ubi Terminator 584 Terminator region from the Sorghum bicolor (sorghum) ubiquitin gene (Phytozome gene ID Sobic.004G049900.1; US Patent 9725731 [Abbitt, 2017]) 7,779 -7,784 Intervening Sequence 6 DNA sequence used for cloning 7,785 -8,827 (complement ary) s / >-actin Terminator 1,043 Terminator region from Sorghum bicolor (sorghum) actin gene (GenBank accession XM_002441128.2; US Patent 9725729 [Abbitt and Jung, 2017]) 8,828 8,937 Intervening Sequence 110 DNA sequence used for cloning crylB.6 1.1 gene cassette 8,938 9,818 os-T28 Terminator 881 Bidirectional terminator region (US Patent 10059953 [Bhyri et al., 2018]) from a Oryza sativa (rice) NAC domain-containing protein gene (Phytozome gene ID LOC_Os03g60080.1) and the methylenetetrahydrofolate reductase gene (Phytozome gene ID LOC 0s03g60090.1) 9,819 9,825 Intervening Sequence 7 DNA sequence used for cloning 9,826 11,796 (complement ary) cry IB. 61.1 1,971 Modified cry IB-class gene derived from Bacillus thuringiensis (WO Patent 2017180715 [Hom et al., 2017]) 11,797 11,812 Intervening Sequence 16 DNA sequence used for cloning 11,813 -11,937 (complement ary) gm-cab3 5' UTR 125 5' UTR region from the Glycine max chlorophyll a / b binding protein gene (Phytozome gene ID Glyma05g25810; US Patent 20200407742 [Sidorenko et al., 2020]) 11,938 13,792 (complement ary) gm-cab3 Promoter 1,855 Promoter region from the Glycine max chlorophyll a / b binding protein gene (Phytozome gene ID Glyma05g25810; US Patent 20200407742 [Sidorenko et al., 2020]) 13,793 -13,886 Intervening Sequence 94 DNA sequence used for cloning 13,887 -13,910 attB2 24 Bacteriophage lambda integrase recombination site from the Invitrogen Gateway® cloning system (Hartley et al., 2000; Katzen, 2007) 13,911 -14,066 Intervening Sequence 156 DNA sequence used for cloning 14,067 -15,063 (complement ary) sb-PEPCl Terminator 997 Terminator region from the Sorghum bicolor (sorghum) phosphoenolpyruvic carboxylase gene (WO Patent 2018102131 [Abbitt et al., 2018]) 15,06415,086 Intervening Sequence 23 DNA sequence used for cloning 15,08716,170 (complement ary) UBQ3 Terminator 1,084 Terminator region from the Arabidopsis thaliana ubiquitin 3 gene (WO Patent 2016149352 [Elsing et al., 2016]) 16,171 -16,333 Intervening Sequence 163 DNA sequence used for cloning ipdO83C b gene cassette 16,334 17,109 os-T17 Terminator 776 Bidirectional terminator region (US Patent 10059953 [Bhyri et al., 2018]) from the Oryza sativa (rice) 5-enolpyruvyl shikimate-3-phosphate synthase gene (Phytozome gene ID LOC_Os06g04280.1) and the ribosomal protein S10 gene (Phytozome gene ID LOC 0s06g04290.1) 17,HO-17,116 Intervening Sequence 7 DNA sequence used for cloning 17,11719,678 (complement ary) ipdO83Cb 2,562 Insecticidal protein gene from Adiantum trapeziforme var. braziliense (giant maidenhair fem) (US Patent 10227608 [Barry et al., 2019]) 19,679 19,697 Intervening Sequence 19 DNA sequence used for cloning 19,698 -19,984 (complement ary) pv-ubi2 Intron 287 Intron region from the Phaseolus vulgaris (common bean) ubiquitin 2 gene (Phytozome gene ID Phvul.003G123900.1) 19,985 -20,092 (complement ary) pv-ubi2 5' UTR 108 5' untranslated region from the Phaseolus vulgaris (common bean) ubiquitin 2 gene (Phytozome gene ID Phvul.003G123900.1) 20,093 -22,667 (complement ary) pv-ubi2 Promoter 2,575 Promoter region from the Phaseolus vulgaris (common bean) ubiquitin 2 gene (Phytozome gene ID Phvul.003G123900.1) 22,668 -22,708 Intervening Sequence 41 DNA sequence used for cloning 22,709 -22,729 (complement ary) attB3 21 Bacteriophage lambda integrase recombination site (Cheo et al., 2004) 22,730 -22,844 Intervening Sequence 115 DNA sequence used for cloning gm-hral gene cassette 22,845 - 23,483 SAMS Promoter 639 Promoter region from the Glycine max S-adenosyl-L-methionine synthetase gene (US Patent 7834242 [Falco and Li, 2010]) 23,484 -23,542 SAMS 5' UTR 59 5' untranslated region from the Glycine max S-adenosyl-L-methionine synthetase gene (US Patent 7834242 [Falco and Li, 2010]) 23,543 - 24,133 SAMS Intron 591 Intron region from the Glycine max S-adenosyl-L-methionine synthetase gene (US Patent 7834242 [Falco and Li, 2010]) 24,134 24,147 SAMS 5' UTR 14 5’ untranslated region from the Glycine max S-adenosyl-L-methionine synthetase gene (US Patent 7834242 [Falco and Li, 2010]) 24,148 24,152 Intervening Sequence 5 DNA sequence used for cloning 24,153 -24,200 FRT1 48 Flippase recombination target site from Saccharomyces cerevisiae (Proteau et al., 1986) 24,201 -24,214 Intervening Sequence 14 DNA sequence used for cloning 24,215 -26,170 gm-hra 1 1,956 Modified acetolactate synthase gene from Glycine max (US Patent 7834242 [Falco and Li, 2010]) 26,171 -26,822 als Terminator 652 Terminator region from the Glycine max acetolactate synthase gene (Phytozome gene ID Glyma.04G196100; US Patent 7834242 [Falco and Li, 2010]) 26,823 -26,979 Intervening Sequence 157 DNA sequence used for cloning 26,980 - 27,669 Z19 Terminator 690 Terminator region from the Zea mays 19-kDa zein gene (GenBank accession KX247647.1; Dong et al., 2016) 27,670 -27,719 Intervening Sequence 50 DNA sequence used for cloning 27,720 -27,776 Ti Plasmid Region 57 Sequence from the Agrobacterium tumefaciens Ti plasmid (GenBank accession AE007871.2; Komari et al., 1996) 27,777 -27,801 Left Border (LB) 25 T-DNA Left Border from the Agrobacterium tumefaciens octopine-type Ti plasmid (GenBank accession AF242881.1; Komari etal., 1996) Example 2. Transformation of Soybean by Agrobacterium transformation and Regeneration of Transgenic Plants

[0193] COR23134 soybean was created by Agyobacterium-vaQ&%\e& transformation of soybean variety 93Y21 with plasmid PHP90315. Immature soybean cotyledons were inoculated with Agrobacterium tumefaciens strain AGL1 containing plasmid PHP90315. Agrobacterium tumefaciens strain AGL1 is a disarmed strain that contains the vir genes and enables efficient transfer of the transfer DNA (T-DNA) region of the transformation plasmid to the inoculated host plant tissue. Healthy green callus was transferred to a solid maturation medium and incubated, followed by desiccation of the resulting embryos. Embryos were then transferred to a solid germination medium to initiate shoot and root development. Once shoots and roots were established, healthy plants were selected, and PCR was used to confirm the presence of the PHP90315 T-DNA insert. Plants that were regenerated from transformation and tissue culture (designated TO plants) were selected for further characterization. Example 3. Identification of Soybean Events COR-23134-4

[0194] Soybean (Glycine max (L.) Merr.) was modified by the insertion of the gm-hra, cry IB. 34, crylB.61, and ipdO83Cb genes via insertion of T-DNA (Figure 2) from plasmid PHP90315 (Figure 1) to create event COR-23134-4 (also referred to as COR23134 soybean).

[0195] Polymerase chain reaction (PCR) amplification of unique regions within the introduced genetic elements can distinguish the test plants from their non-genetically modified counterparts and can be used to screen for the presence of the inserted T-DNA region of plasmid PHP90315.

[0196] Real-time PCR analyses of COR23134 soybean using event-specific and construct-specific assays confirmed the COR23134 soybean plants from two segregating generations (T2 and T3) contained the inserted T-DNA regions of plasmid PHP90315, as demonstrated by the Ct values produced in all test plants analyzed and confirmed the absence of event COR-23134-4 in the negative segregant plants within each generation. These results also indicate stable integration and segregation of a single copy of the inserted genes with transfer to subsequent generations. The data were highly reproducible across technical replicates of the plants tested. The soybean endogenous reference gene assay for detection of the Lectin (Lei} gene amplified, as expected, for all the plants analyzed.

[0197] The sensitivity of the construct-specific PCR detection methods in COR23134 soybean, under the conditions performed in 5 ng of soybean genomic DNA, has demonstrated ability to detect to approximately 5 pg of the gm-hra gene, 20 pg of the cry IB. 34 gene, 10 pg of the cry IB.61 gene, 5 pg of the ipdO83Cb gene, and 10 pg of the COR-23134-4 event. These concentrations are equivalent to 0.1%, 0.4%, 0.2%, 0.1%, and 0.2% of the COR23134 soybean genomic DNA, respectively.

[0198] For detection of the gm-hra, cry IB. 34, crylB.61, and ipdO83Cb genes contained within COR23134 soybean, regions spanning between 57 bp and 109 bp were amplified 53 using primers and Taqman® probes specific for each unique sequence. Additionally, a 66-bp region of an endogenous reference gene, Lectin (LeT, GenBank accession number K00821.1), was validated to be used in duplex with each assay for both qualitative and quantitative assessment of each assay and to demonstrate the presence of sufficient quality and quantity of DNA within the PCR reaction (Kuribara et al., 2002). Ct data from Lei was used in calculations regarding scoring with regard to the event or gene tested. Data were compared to the performance of either the validated positive or copy number calibrator as well as negative genomic controls as described below.

[0199] The real-time PCR reaction exploited the 5’ nuclease activity of the heat-activated DNA polymerase. Two primers and one probe annealed to the target DNA with the probe, which contained a 5’ fluorescent reporter dye and a 3’ quencher dye. With each PCR cycle, the reporter dye was cleaved from the annealed probe by the polymerase, emitting a fluorescent signal that intensified with each subsequent cycle. The cycle at which the emission intensity of the sample amplicon rose above the detection threshold was referred to as the Ct value. When no amplification occurred, there was no Ct calculated by the instrument and was assigned a Ct value of 40.00.

[0200] Samples tested qualitatively were determined to be positive or negative for a specific gene of interest using the following criteria: • Positive: o Endogenous gene Ct < 35 o Gene of interest (GOI) Ct < 35 o ACt (Endogenous Ct - GOI Ct) > -5 • Negative: o Endogenous gene Ct < 35 o Gene of interest (GOI) Ct > 35 o ACt (Endogenous Ct - GOI Ct) < or > -5

[0201] If copy number of the test samples was to be determined to determine a quantitative result, copy number calibrators (samples known to contain defined copies of the gene of interest, e.g., 1 or 2 copies) were used as controls for both the endogenous gene and gene of interest. Fold differences were used to apply a copy number for each test sample. Fold difference, or fold change, is calculated using the formula of 2'ACT. The ACt was calculated for the test samples and copy number calibrators as described above. A copy number of 1 was applied to the sample population producing a fold change between 0 and 0.7 with a maximum range of 0.75 when compared to the 2-copy calibrators. Likewise, a copy number of 2 was applied to a sample population producing a fold change ranging between 1.5 and 2.2 with a maximum range of 0.91 when compared to the single copy calibrators; and a copy number of 3 was applied to a sample population producing a fold change ranging between 1.3 and 1.5 with a maximum range of 0.35 when compared to the 2-copy calibrators.

[0202] Genomic DNA was isolated from COR23134 soybean leaf tissue for 58 and 81 plants from each of the T2 and T3 generations, respectively. The DNA samples were extracted using a high alkaline buffer comprised of sodium hydroxide, ethylenediaminetetraacetic acid disodium salt dihydrate (Na2-EDTA), and Tris hydrochloride. Approximately 3 ng of template DNA were used per reaction.

[0203] Each assay supporting event COR-23134-4, as well as the transgenes contained within event COR-23134-4 soybean, were multiplexed with the Lei endogenous reference assay. Reaction mixes were prepared, each comprised of all components to support both the gene of interest and the endogenous gene for the PCR reaction. The base master mix, Taqman SureAmp™ Lo-ROX master mix, was used. Individual concentrations of primer varied per assay between 300 nM and 900 nM, dependent on the optimal concentration established for the specific target and the endogenous reference during analysis validation. Individual concentrations of probe per assay were either 80 nM or 120 nM. Assay controls included no template controls (NTC) which consisted of molecular biology grade water or Tris-EDTA (TE) buffer (10 mM Tris pH 8.0, ImM EDTA) as well as copy number calibrator and negative controls, all of which were validated for each assay performed. Annealing temperatures and number of cycles used during the PCR analyses are provided in Tables 2 and 3. The primer and probes used for each PCR analysis are provided in Tables 4 and 5. Master mix formulations for each PCR analysis are provided in Tables 6-10. PCR Parameters

[0204] The PCR parameters used during PCR analysis are listed below: Table 2. Annealing Temperatures and Cycles used During the PCR Reaction (COR-23134-4 and ipdO83Cb) Step Description Temperature (°C) Time (seconds) Cycles 1 UNG Step 50 120 1 2 Initial Denaturation 92.5 60 1 3a Amplification Denaturation 92.5 5 40 3b Anneal / Extend 58 30 Table 3. Annealing Temperatures and Cycles used During the PCR Reaction (gm-hra, cry IB. 34, and cry IB. 61) Step Description Temperature (°C) Time (seconds) Cycles 1 UNG Step 50 120 1 2 Initial Denaturation 92.5 20 1 3a Amplification Denaturation 92.5 1 40 3b Anneal / Extend 60 15 Primers and Probes

[0205] The primers and probe used for each transgene are listed in Table 4. The primers and probe used for the Lei endogenous reference gene are listed in Table 5. Table 4. Primers and Probe for PCR Analysis of the COR-23134-4 Soybean Event and gm-hra, cry IB. 34, cry IB. 61, and ipdO83Cb Genes Reagent Sequence (5’ to 3’) Length (base) SEQID NO: 18 COR-23134-4 forward primer GACTTGCGTTAGTTGTGTTAGAATGTC 27 SEQID NO: 19 COR-23134-4 reverse primer TGATCCCGATTTTTTTGATGTTT 23 SEQID NO: 20 COR-23134-4 probe 6-FAM-CGGATAGTTATATTTGTGCCAAC-MGB / NFQ 23 SEQID NO: 21 gm-hra forward primer TTCTGGAAGCGGTGGCC 17 SEQID NO: 22 gm-hra reverse primer GTGCAGTTTTTGAAGTATAACAACAGAAGT 30 SEQID NO: 23 gm-hra probe 6-FAM-CATTGTGTTGTGTGGTGGA-MGB / NFQ 19 SEQID NO: 24 cry IB. 34 forward primer AGGCAGACTTGTGAACCCTAGAAC 24 SEQID NO: 25 cry IB. 34 reverse primer GACCTGGAGAGAGCCCAGAAG 21 SEQID NO: 26 cry IB. 34 probe 6-FAM-AGCAGCTGTTGTATTAGG-MGB / NFQ 18 SEQID NO: 27 cry IB. 61 forward primer GTCGGCGTCCGCAACTT 17 SEQID NO: 28 cry IB. 61 reverse primer GAACTCGAAGCGGTCGATGA 20 SEQID NO: 29 cry IB. 61 probe 6-FAM-TCCGAGAACGCCGGAGT-MGB / NFQ 17 SEQID NO: 30 ipdO83Cb forward primer CCATATTGATCTACCACTCGAAACA 25 SEQID NO: 31 ipdO83Cb reverse primer TCGTACTTTGGCGGTGTGAA 20 SEQID NO: 32 ipdO83Cb probe 6-FAM-CAACGGCATTCAGTG-MGB / NFQ 15 SEQID NO: 33 COR-2313Z in bold and GACTTG( -4 assay amplicon sequence (80-bp; primer and probe binding sites are underlined) 7GTTAGTTGTGTTAGAATGTCCATCGGATAGTTATATTTG TGCCAACACCAAAACATCAAAAAAATCGGGATCA SEQID NO: 34 gm-hra assay amplicon sequence (109-bp; primer and probe binding sites are in bold and underlined) GTGCAGTTTTTGAAGTATAACAACAGAAGTTCCTATTCCGAAGTTCC TATTCTCTAGAAAGTATAGGAACTTCCACCACACAACACAATGGCGG CCACCGCTTCCAGAA SEQID NO: 35 cry IB. 34 assay amplicon sequence (67-bp; primer and probe binding sites are bold and underlined) GACCTGGAGAGAGCCCAGAAGGCCTAATACAACAGCTGCTGCTGTT CTAGGGTTCACAAGTCTGCCT SEQID NO: 36 cry IB.61 assay amplicon sequence (57-bp; primer and probe binding sites are in bold and underlined) GAACTCGAAGCGGTCGATGATCACTCCGGCGTTCTCGGAGAAGTT GCGGACGCCGAC SEQID NO: 37 ipdO83Cb assay amplicon sequence (68-bp; primer and probe binding sites are in bold and underlined) TCGTACTTTGGCGGTGTGAAACACTGAATGCCGTTGGGGAATCTGT TTCGAGTGGTAGATCAATATGG Table 5. Primers and Probe for PCR Analysis of the Lei Endogenous Reference Gene Reagent Sequence (5’ to 3’) Length (base) SEQIDNO: 38 Lei forward primer TCCCGAGTGGGTGAGGATAG 20 SEQ ID NO: 39 Lei reverse primer TCATGCGATTCCCCAGGTAT 20 SEQ ID NO: 40 Lei probe VIC-TTCTCTGCTGCCACGGGACTCGA-QSY 23 SEQIDNO: 41 Le\ assay amp bold and under TCCCGAGD icon sequence (66-bp; primer and probe binding sites are in lined) GGGTGAGGATAGGGTTCTCTGCTGCCACGGGACT CGACATACCTGGGGAATCGCATGA Preparation of Master Mix

[0206] The components and concentrations supporting each master mix are listed below: Table 6. Master Mix Supporting Multiplex Assay: COR-23134-4 and Lei Component Stock Concentration Final Concentration Volume / reaction (pL)a Taqman Sure Amp™ Lo-ROX master mix 2x lx 3 COR-23134-4 forward primer 10 pM 600 nM 0.018 COR-23134-4 reverse primer 10 pM 600 nM 0.018 COR-23134-4 probe 10 pM 120 nM 0.007 Lei forward primer 10 pM 300 nM 0.009 Lei reverse primer 10 pM 300 nM 0.009 Lei probe 10 pM 120 nM 0.007 HPLC Molecular Biology Grade Water N_Ab N_Ab 1.932 a Final volume of each reaction was 6 pL comprised of 5.0 pL of Master Mix and 1.0 pL of genomic DNA template. b N_A is equivalent to Not Applicable. Table 7. Master Mix Supporting Multiplex Assay: gm-hra and Lei Component Stock Concentration Final Concentration Volume / reaction (plf SensiFast™ probe Lo-ROX master mix 2x lx 3 gm-hra forward primer 10 pM 600 nM 0.018 gm-hra reverse primer 10 pM 600 nM 0.018 gm-hra probe 10 pM 120 nM 0.007 Lei forward primer 10 pM 900 nM 0.027 Lei reverse primer 10 pM 900 nM 0.027 Lei probe 10 pM 80 nM 0.005 HPLC Molecular Biology Grade Water N_Ab N_Ab 1.898 a Final volume of each reaction was 6 pL comprised of 5.0 pL of Master Mix and 1.0 pL of genomic DNA template. b N_A is equivalent to Not Applicable. Table8. Master Mix Supporting Multiplex Assay: crylB.34 and Lei Component Stock Concentration Final Concentration Volume / reaction (plf SensiFast™ probe Lo-ROX master mix 2x lx 3 cry IB. 34 forward primer 10 pM 600 nM 0.018 cry IB. 34 reverse primer 10 pM 600 nM 0.018 cry IB. 34 probe 10 pM 120 nM 0.007 Lei forward primer 10 pM 600 nM 0.018 Lei reverse primer 10 pM 600 nM 0.018 Lei probe 10 pM 120 nM 0.007 HPLC Molecular Biology Grade Water N_Ab N_Ab 1.914 a Final volume of each reaction was 6 pL comprised of 5.0 pL of Master Mix and 1.0 pL of genomic DNA template. b N_A is equivalent to Not Applicable. Table 9. Master Mix Supporting Multiplex Assay: crylB.61 and Lei Component Stock Concentration Final Concentration Volume / reaction (plf SensiFast™ probe Lo-ROX master mix 2x lx 3 cry IB. 61 forward primer 10 pM 300 nM 0.009 cry IB. 61 reverse primer 10 pM 300 nM 0.009 cry IB. 61 probe 10 pM 120 nM 0.007 Lei forward primer 10 pM 600 nM 0.018 Lei reverse primer 10 pM 600 nM 0.018 Lei probe 10 pM 120 nM 0.007 HPLC Molecular Biology Grade Water N_Ab N_Ab 1,932 a Final volume of each reaction was 6 pL comprised of 5.0 pL of Master Mix and 1.0 pL of genomic DNA template. b N_A is equivalent to Not Applicable. Table 10. Master Mix Supporting Multiplex Assay: ipdO83Cb and Lei Component Stock Concentration Final Concentration Volume / reaction (plf SensiFast™ probe Lo-ROX master mix 2x lx 3 ipdO83Cb forward primer 10 pM 900 nM 0.027 ipdO83Cb reverse primer 10 pM 900 nM 0.027 ipdO83Cb probe 10 pM 120 nM 0.007 Lei forward primer 10 pM 300 nM 0.009 Lei reverse primer 10 pM 300 nM 0.009 Lei probe 10 pM 120 nM 0.007 HPLC Molecular Biology Grade Water N_Ab N_Ab 1.914 a Final volume of each reaction was 6 pL comprised of 5.0 pL of Master Mix and 1.0 pL of genomic DNA template. b N_A is equivalent to Not Applicable. PCR Analysis

[0207] Genomic DNA samples isolated from collected leaf samples of 151 COR23134 soybean plants (582 plants from the T2 and 813 plants from the T3 generations) along with copy number calibrator, negative, and NTC controls, were subjected to qPCR amplification using TaqMan SureAmp™Lo-ROX master mix (ThermoFisher; California, USA) in the presence of primer pair and probes specific for genes gm-hra, cry IB. 34, crylB.61, and ipdO83Cb as well as the COR-23134-4 event which allowed for the unique identification of the PHP90315 T-DNA insertion in COR23134 soybean. For assay and DNA quality monitoring, soybean Lei was included in duplex with each reaction as an endogenous control. Each qPCR reaction was set up in a total volume of 6 pL with approximately 3 ng (1.0 pL of volume) of the isolated genomic DNA. Copy number results for the T2 and T3 generations tested are provided in Table 11. Table 11. Copy Number Determination of Event COR-23134-4 and the gm-hra, crylB.34, crylB.61 and ipdO83Cb Genes in Two Generations of COR23134 Soybean Event Generation Number Analyzed Event / T ransgene Average Ct Average ACt Copy Number COR-23134-4 T2, Entry 2 26 COR-23134-4 29.70 -2.97 1 14 28.73 -2.04 2 18 39.76 -13.91 Null 26 gm-hra 27.56 0.03 1 14 26.64 0.87 2 18 33.89 -7.08 Null3 26 cry IB. 34 28.23 -1.47 1 14 27.28 -0.50 2 18 39.75 -13.87 Null 26 Cry IB. 61 27.25 0.84 1 14 26.30 1.85 2 18 39.52 -12.24 Null 26 ipdO83Cb 28.13 -1.24 1 14 27.15 -0.41 2 18 40.00 -13.97 Null T3, Entry 3 48 COR-23134-4 30.22 -2.98 1 13 29.13 -2.12 2 20 40.00 -12.73 Null 48 gm-hra 27.92 0.04 1 13 26.89 0.86 2 20 33.72 -5.70 Null3 48 cry IB. 34 28.64 -1.42 1 13 27.48 -0.52 2 20 39.84 -12.59 Null 48 cry IB. 61 27.66 0.91 1 13 26.52 1.87 2 20 40.00 -11.44 Null 48 ipdO83Cb 28.59 -1.28 1 13 27.44 -0.38 2 20 40.00 -12.78 Null 1 6 plants were omitted from the T2 generational data due to ambiguous copy number calls. 2 6 plants were omitted from the T3 generational data due to ambiguous copy number calls. 3 Although some late and non-linear amplification was detected in the Null scoring samples, the calculated fold change using both the Calibrator 1 and Calibrator 2 genomic controls was outside of the maximum range for a 1 or 2 copy number assignment resulting in Null final scores. Event-Specific and Construct-Specific PCR Analyses for COR23134 Soybean

[0208] The results of the qPCR copy number analyses indicate stable integration and segregation of a single copy of the genes within the T-DNA of plasmid PHP90315, with demonstrated transfer to subsequent generations.

[0209] PCR products ranging in size between 57 bp to 109 bp, representing event COR-23134-4 as well as the genes within the T-DNA from plasmid PHP90315, were amplified and observed in leaf samples of COR23134 soybean as well as in eight copy number 1 calibrator genomic controls and eight copy number 2 genomic controls but were absent in each of the four negative genomic controls and four NTC controls.

[0210] Using the soybean endogenous reference gene Lei, a PCR product of 66 bp was amplified and observed in leaf samples from COR23134 soybean as well as in eight copy number 1 calibrator, eight copy number 2 calibrator, and four negative genomic controls. Amplification of the endogenous gene was not observed in the four NTC controls tested with no generation of Ct values.

[0211] For each sample, all assays were performed in duplex (specific target with endogenous reference gene), analyzing for the insertion site and all genes. For each sample, Ct values, ACt values, and copy numbers (if applicable) were calculated. Sensitivity of Construct-Specific PCR Analyses for COR23134 Soybean

[0212] To assess the sensitivity of the construct-specific PCR assays, COR23134 soybean DNA was diluted in control soybean genomic DNA, resulting in test samples containing various amounts of COR23134 soybean (5 ng, 1 ng, 500 pg, 250 pg, 100 pg, 50 pg, 20 pg, 10 pg, 5 pg, and 0 pg) in a total of 5 ng soybean DNA. These various amounts of COR23134 soybean DNA correspond to 100%, 20%, 10%, 5%, 2%, 1%, 0.4%, 0.2%, 0.1%, and 0% of COR23134 soybean DNA in total soybean genomic DNA, respectively. The 62 various amounts of COR23134 soybean DNA were subjected to real-time PCR amplification for gm-hra, cry IB. 34, crylB.61, and ipdO83Cb genes and the COR-23134-4 soybean event. Based on these analyses, the limit of detection (LOD) in 5 ng of total DNA for COR23134 soybean was determined to be approximately 5 pg for gm-hra (0.1%), 20 pg for crylB.34 (0.4%), 10 pg for cry IB.61 (0.2%), 5 pg for ipdO83Cb (0.1%), and 10 pg for COR23134 soybean (0.2%). The determined sensitivity of each assay described is sufficient for many screening applications. Each concentration was tested a total of five times. At the point where amplification of the target tested was not detected in each replicate, the preceding concentration was determined to be the limit of sensitivity.

[0213] Real-time PCR analyses of COR23134 soybean DNA using event-specific and construct-specific assays confirm the stable integration and segregation of a single copy of the T-DNA of plasmid PHP90315 in leaf samples tested, as demonstrated by the quantified detection of event COR-23134-4 and the gm-hra, cry IB. 34, crylB.61, and ipdO83Cb genes in COR23134 soybean plants. These results were reproducible among all the replicate qPCR analyses conducted. The soybean endogenous reference gene assay for detection of Lei amplified as expected in all the test samples and negative controls and was not detected in the NTC samples. The sensitivity of each assay under the conditions described ranged between 5 pg to 20 pg DNA, all sufficient for many screening applications by PCR. Example 4. Southern-by-Sequencing (SbS) Analysis of COR-23134-4 soybean for the Insertion Organization and Copy Number

[0214] An application of Next-generation sequencing (NGS) called Southern-by-Sequencing (SbS) analysis was conducted on the TO generation of COR-23134-4 soybean to demonstrate that a single insertion has occurred in soybean event COR-23134-4. .

[0215] Genomic DNA was extracted from leaf tissue of the TO generation of COR-23134-4 soybean. SbS utilizes probe-based sequence capture, Next Generation Sequencing (NGS) techniques, and bioinformatics procedures to isolate, sequence, and identify inserted DNA within the soybean genome. By compiling a large number of unique sequencing reads and comparing them to the transformation plasmid sequence and the soybean genome, unique junctions resulting from inserted DNA are identified in the bioinformatics analysis and can be used to determine the number of insertions within the plant genome. A single insertion will have two genomic-insertion junctions, one at each end of the insertion. The TO plant of COR-23134-4 soybean was analyzed by SbS to determine the copy number and integrity of the insertion.

[0216] A series of unique sequences encompassing the PHP90315 plasmid sequence was used to design overlapping biotinylated oligonucleotides as capture probes. The capture probes were designed and synthesized by Roche NimbleGen, Inc. Sequences in the COR-23134-4 genome that match the PHP90315 transformation plasmid, either from the T-DNA insertion or endogenous soybean sequences similar to elements in PHP90315, would be enriched by hybridization to the probes during the capture process. The probes were compared to the soybean genome to determine the level of soybean genomic sequence that would be captured and sequenced simultaneously with sequences derived from PHP90315.

[0217] Separate NGS libraries were constructed for COR-23134-4 soybean and control soybean. SbS was performed essentially as described in Zastrow-Hayes, et al (2015). The sequencing libraries were hybridized to the capture probes through two rounds of hybridization to enrich the targeted sequences. Following NGS (Illumina NextSeq), the sequencing reads entered the bioinformatics pipeline for trimming and quality assurance. Reads were aligned against the soybean genome and the T-DNA region from plasmid PHP90315 to determine the copy number and insertion organization derived from the T-DNA. Reads were also aligned to the full sequence of PHP90315 to determine if any additional plasmid sequences were incorporated into the genome. Reads that contained both genomic and plasmid sequence and reads that contained noncontiguous plasmid sequence were identified as junction reads.

[0218] To identify putative junctions that were due to endogenous soybean sequences, a control soybean genomic DNA library was separately captured and sequenced in the same manner as the TO COR-23134-4 soybean plant. This library was sequenced to approximately the same average depth as the COR-23134-4 soybean plant sample. This increased the probability that the endogenous junctions captured by the probes would be detected in the control sample, so that they could be identified and removed in the COR-23134-4 soybean sample.

[0219] Copy number of the DNA insertion in COR-23134-4 soybean, derived from plasmid PHP90315 (Figure 1), was determined. A schematic map of the PHP90315 T-DNA is provided in Figure 2.

[0220] SbS was conducted on the TO plant of COR-23134-4 soybean to determine the copy number and the insertion organization in the genome. Alignments of the SbS sequencing reads to the T-DNA from PHP90315 resulted in two unique plasmid-genome junctions between the flanking genomic sequence and the inserted DNA, indicating the presence of a single insertion in COR-23134-4 soybean. A single plasmid-plasmid junction was identified within the insertion, indicating that there is a 21-bp deletion in the pv-ubi2 promoter of the ipdO83Cb gene cassette that differs from the PHP90315 T-DNA sequence. There were no additional junctions between the PHP90315 sequence and the soybean genome detected in the TO plant, indicating that there are no additional plasmid-derived insertions present in COR-23134-4 soybean. Furthermore, there were no junctions between soybean genome sequences and the backbone sequence of PHP90315 in the TO plant, demonstrating that no plasmid backbone sequences were incorporated into COR-23134-4 soybean. Example 5. Insect efficacy of soybean events COR-23134-4

[0221] Soybean (Glycine max [L.] Merr.) event COR-23134-4 (referred to as COR23134 soybean) expresses the Cry IB.34.1, Cry IB.61.1, and IPD083Cb proteins for control of certain susceptible lepidopteran pests, and the GM-HRA protein that was used as a selectable marker.

[0222] Greenhouse experiments were conducted to evaluate efficacy of event COR-23134-4 (containing insecticidal proteins Cry IB.34.1, Cry IB.61.1, and IPD083Cb) in Toledo, Brazil over 2 years. A separate experiment was conducted for each of the three Lepidopteran target species: velvetbean caterpillar (Anticarsia gemmatalis), soybean looper (Chrysodeixis includens), and fall army worm (Spodoptera frugiperda).

[0223] The two treatments were COR23134 and wild type soybean, both in soybean variety 93Y21. The experiments were arranged in a randomized complete block design with three replications. Each plot consisted of 16 plants.

[0224] For all species, insects were manually infested at uniform rates to each plot when soybean plants reached the R2 growth stage. Visual ratings for percent defoliation (0-100%) were assessed on each plot approximately 14 days after initial feeding was observed. Statistical analysis was conducted using linear mixed models to evaluate percent defoliation results for COR23134 soybean and wild type soybean. Table 12. Lepidopteran Target Species Target Species Scientific Name Family Insect Source Velvetbean Caterpillar (2021) Anticarsia gemmatalis Erebidae Manually Infested Soybean Looper(2022) Chrysodeixis includens Noctui dae Manually Infested Fall Army worm (2022) Spodoptera frugiperda Noctui dae Manually Infested Statistical Analysis

[0225] A linear mixed model was applied to model percent defoliation for each experiment separately. Data for percent defoliation (Yipmns) of replication (R)i, protein (P) / ?, construct (Qm, event (E)n and plot s, were modeled as a function of an overall mean / / , factors for replication, construct, event, construct by event by and a residual aPmns. The model can be specified as: Yipmns = p + RI + Pp + (C x £)mn + Sipmns where protein was treated as fixed effect, and all the other effects except the residual were treated as independent normally distributed random variables with means of zero.

[0226] For the residual, instead of assuming independence among plots, 2-dimensional separable first-order autoregressive correlation (ARI X ARI) structure was applied to capture plot-to-plot correlations in both row and column directions of the field, besides the plot-to-plot variation, / '-tests using standard errors from the model were conducted to compare treatment effects. A difference was considered statistically significant if the P-value of the difference was less than 0.05. All data analysis and comparisons were made in ASReml 4.0 (VSN International, Hemel Hempstead, UK, 2009). Results

[0227] Mean percent defoliation injury results are summarized below for velvetbean caterpillar (Table 13), soybean looper (Table 14), and fall armyworm (Table 15) as means, standard errors, and P-values. In all experiments, percent defoliation injury was significantly higher in the wild type soybean with > 70% defoliation compared to the COR23134 soybean which was < 4.7%. Results from these studies demonstrate that event COR-23134-4 provides protection from velvetbean caterpillar, soybean looper, and fall army worm. Table 13. Insect Efficacy Results for COR23134 Soybean: Percent Defoliation Injury from Velvetbean Caterpillar Soybean Line Mean Percent Defoliation Standard Error P-Value COR23134 Soybean 0.1 0.1 <0.0001* Wild Type Soybean 70 0.3 — ote: Means were estimated from the linear mixed mot el. * A statistically significant difference (P-value < 0.05) was observed. Table 14. Insect Efficacy Results for COR23134 Soybean: Percent Defoliation Injury from Soybean Looper Soybean Line Mean Percent Defoliation Standard Error P-Value COR23134 Soybean 1 2.6 <0.0001* Wild Type Soybean 100 2.6 — Note: Means were estimated from the linear mixed model. * A statistically significant difference (P-value < 0.05) was observed. Table 15. Insect Efficacy Results for COR23134 Soybean: Percent Defoliation Injury from Fall Armyworm Soybean Line Mean Percent Defoliation Standard Error P-Value COR23134 Soybean 4.7 4.4 <0.0001* Wild Type Soybean 96.7 4.4 — Note: Means were estimated from the linear mixed model. * A statistically significant difference (P-value < 0.05) was observed. Example 6. Agronomic and yield field evaluations of soybean events COR-23134-4 Agronomic Characteristics of a Soybean Line Containing Event COR-23134-4

[0228] Soybean (Glycine max [L.] Merr.) event COR-23134-4 (referred to as COR23134 soybean) expresses the Cry IB.34.1, Cry IB.61.1, and IPD083Cb proteins for control of certain lepidopteran pests and the GM-HRA protein that was used as a selectable marker.

[0229] The objective of this study phase was to evaluate agronomic characteristics of COR23134 soybean - both for untreated COR23134 soybean or for COR23134 soybean treated with diclosulam (referred to as diclosulam-treated COR23134 soybean).

[0230] The field portion of this study was conducted during the 2022 growing season at 12 sites in commercial soybean-growing regions of the United States (two sites in each of Iowa, Illinois, and Indiana, and one site in each of Missouri, Nebraska, Pennsylvania, and Wisconsin) and Canada (two sites in Ontario). A randomized complete block design with four blocks was utilized at each site. Each block included untreated COR23134 soybean, diclosulam-treated COR23134 soybean, non-genetically modified (non-GM) near-isoline control soybean (referred to as control soybean), and four out of eighteen non-GM commercial soybean lines (referred to as reference soybean).

[0231] Data were collected for the following agronomic endpoints: early stand count, days to flowering, plant height, days to maturity, lodging, shattering, final stand count, pod count, yield, harvest seed moisture, and 100-seed weight. Statistical analyses were conducted to evaluate and compare agronomic results derived from COR23134 soybean to the control soybean.

[0232] The results obtained in this study phase demonstrated that agronomic characteristics of COR23134 soybean were comparable to those of conventional soybean represented by non-GM near-isoline control soybean and non-GM commercial soybean. For untreated COR23134 soybean, a statistically significant difference was identified in pod count, with 46 of 48 observations within the reference range. For diclosulam-treated COR23134 soybean, a statistically significant difference was identified in early stand count, with 47 of 48 observations within the reference range. Materials

[0233] The test system in this study was soybean (Glycine max [L.] Merr.). The test substance consisted of event COR-23134-4 contained within soybean seed. The study included a non-genetically modified (non-GM) near-isoline soybean line (referred to as control soybean), which did not contain event COR-23134-4. Additionally, a total of 18 68 non-GM commercial soybean lines (referred to as reference soybean) were included in the study. Methods Experimental Design

[0234] The field portion of this study was conducted during the 2022 growing season at 12 sites in commercial soybean-growing regions of the United States (two sites in each of Iowa, Illinois, and Indiana, and one site in each of Missouri, Nebraska, Pennsylvania, and Wisconsin) and Canada (two sites in Ontario). A randomized complete block design with four blocks was utilized at each site. Each block included untreated COR23134 soybean, diclosulam-treated COR23134 soybean, non-genetically modified (non-GM) near-isoline control soybean (referred to as control soybean), and four of the following non-GM commercial soybean lines (referred to as reference soybean): 92M35, 92B63, 92M72, BK291, P29T50, BK310, BK317, BK331N, P33T60, BK340, 93Y41, P34A50, P35A41, BK360, BK361, 93M62, BK370, and 93B82 soybean.

[0235] Bias in the generation of agronomic data in this study was controlled by randomization of the entries within each block and uniform maintenance treatments across all plots at each site. Field Trial Planting

[0236] Each block contained COR23134 soybean, control soybean, and four reference soybean lines planted in 4-row plots at a rate of approximately 168 seeds per row. Each row was approximately 7.6 m (25 ft) in length and approximately 76 cm (30 in.) in width at all sites, with the exception of site IN3, where row length was 6.1m (20 ft). Each block was separated by an alley of at least 0.9 m (36 in.) in width, and each plot was bordered on either side by one row of soybeans. Maintenance Product Applications

[0237] At a given site, maintenance products were uniformly applied, as needed, to all plots to minimize weed, insect, and disease pressure. Glyphosate and glufosinate herbicides were not used post emergence as maintenance applications in this study. Diclosulam, quizalofop, and fomesafen herbicides and insecticides containing Bacillus thuringiensis (Bl) were not used as maintenance applications in this study. Herbicide Treatment Untreated COR23134 soybean:

[0238] This COR23134 soybean entry is referred to as “untreated” because it was only treated with herbicides labelled for use in conventional soybean and was not treated with diclosulam. The untreated COR23134 soybean plots, as well as all control soybean and reference soybean plots in each block at all field sites were treated with the herbicides quizalofop and fomesafen at the V3-V4 growth stage. Herbicide applications were applied at the target rates at all sites, with the exception of site IL5, where fomesafen was applied at 115% of the target rate.

[0239] A visual evaluation of the plants was completed 12-17 days after each treatment to confirm that no unexpected herbicide injury was observed. As expected, minor foliar leaf burn from the fomesafen application was observed at some sites resulting in no negative impact to plant growth and development. Diclosulam-treated soybean study:

[0240] While a quizalofop and fomesafen herbicide treatment was applied to the untreated COR23134 soybean plot and all control and reference soybean plots at the V3-V4 growth stage, the herbicide diclosulam was applied at the V3-V4 growth stage to the diclosulam-treated plot of COR23134 soybean in each block. Herbicide applications were applied at the target rates at all sites, with the exception of site IL5, where fomesafen was applied at 115% of the target rate.

[0241] A visual evaluation of the plants was completed 12-17 days after each treatment to confirm that no unexpected herbicide injury was observed. As expected, minor foliar leaf burn from the fomesafen application was observed at six of the 12 sites resulting in no negative impact to plant growth and development. Very minor herbicide injury (leaf cupping or chlorosis) from the diclosulam application was observed in plots of herbicide-treated COR23134 soybean at two of the 12 sites resulting in no negative impact to plant growth and development. Agronomic Characteristics Data Collection

[0242] Agronomic characteristics were evaluated at given soybean growth stages for each plot. The following characteristics were evaluated: Early Stand Count

[0243] The total number of plants emerged in each of three marked sections (each one row by one meter) in Rows 1-2 was determined between the VC and V2 growth stages. Additional calculations are provided in the statistical analysis section below. Days to Flowering

[0244] The date when at least one flower was open for at least 50% of the plants in Rows 12 was recorded. These dates were used in subsequent statistical analysis to calculate days to flowering in the statistical analysis section below. Plant Height

[0245] Plant height was measured in centimeters from the soil surface to the uppermost node on the main stem at the R8 growth stage for five individual plants in Rows 1-2. Additional calculations are provided in the statistical analysis section below. Days to Maturity

[0246] The date when 95% of the pods in Rows 1-2 were at the physiological mature color (R8 growth stage) was recorded. These dates were used in subsequent statistical analysis to calculate days to maturity in the statistical analysis section below. Lodging

[0247] Lodging was recorded as the percentage of plants in Rows 1-2, to the nearest 10%, inclined more than 45° from vertical at the R8 growth stage. Pod Count Untreated COR23134 soybean:

[0248] The number of pods (containing seed) per plant from five plants in Rows 1-2 was recorded at the R8 growth stage. Additional calculations are provided in the statistical analysis section below. The same five plants were utilized for pod count and shattering data collection. Diclosulam-treated soybean:

[0249] The number of pods (containing seed) per plant from five plants in Rows 1-2 was recorded at the R8 growth stage. Due to an error in data collection, pod count data was not collected for one plot of diclosulam-treated COR23134 soybean at site MO2. Additional calculations are provided in the statistical analysis section below. The same five plants were utilized for pod count and shattering data collection. Shattering

[0250] The number of shattered pods per plant from five plants in Rows 1-2 was recorded at the R8 growth stage. Additional calculations are provided in the statistical analysis section below. Final Stand Count

[0251] The total number of plants emerged in each of three marked sections (each one row by one meter) in Rows 1-2 was determined at the R8 growth stage. Each section was previously used to evaluate early stand count. Additional calculations are provided in the statistical analysis section below. Yield Untreated COR23134 soybean:

[0252] The seed from all plants in Rows 1 and 2 of each plot was harvested at the R8 growth stage. The weight of the seed was recorded in kilograms. Using the harvest seed moisture, seed weight values from all sites were adjusted to a standardized moisture content and used to calculate yield during subsequent statistical analysis. Rows 1 and 2 of one plot of COR23134 soybean at site ON3 were adjusted to a length of 5.6 m (18 ft) for yield calculations due to missing plants from a fertilizer spill. Diclosulam-treated soybean:

[0253] The seed from all plants in Rows 1 and 2 of each plot was harvested at the R8 growth stage. The weight of the seed was recorded in kilograms. Using the harvest seed moisture, seed weight values from all sites were adjusted to a standardized moisture content and used to calculate yield during subsequent statistical analysis. Harvest Seed Moisture

[0254] The moisture content (%) of harvested seed from Rows 1 and 2 at the R8 growth stage was recorded. 100-Seed Weight

[0255] The weight (g) of 100 seeds from the seed harvested from Rows 1 and 2 at the R8 growth stage was recorded. 100-seed weight data were collected on the same day as harvest seed moisture data collection at all sites, with the exception of site Wil, where 100-seed weight data were collected one day after harvest seed moisture. 100-seed weight values were adjusted to a standardized moisture content (see statistical analysis section below). Statistical Analysis

[0256] Statistical analyses were conducted to evaluate and compare agronomic characteristics of untreated COR23134 soybean and diclosulam-treated COR23134 soybean to the control soybean. Processing of Data Early Stand Count and Final Stand Count

[0257] For early stand count and final stand count, the recorded count values for three separate one meter row lengths were each divided by the count area to calculate the number of plants per square meter. The calculated values were then used to calculate the plot average. Days to Flowering and Days to Maturity

[0258] For days to flowering data, the number of days was calculated from the recorded planting date to the recorded flowering date. For days to maturity data, the number of days was calculated from the recorded planting date to the recorded maturity date. Plant Height, Pod Count, and Shattering

[0259] For plant height, pod count, and shattering data, the recorded values for five individual plants were used to calculate the plot average. Yield

[0260] Yield was determined based on the weight of seed collected at typical harvest maturity as follows: Seed weight was adjusted to 0% moisture content (seed dry weight): Seed dry weight (lb) = Seed fresh weight (lb) x [(100 - % actual moisture) / 100] Seed dry weight was then adjusted to 13% moisture content: Seed weight at 13% moisture (lb) = Seed dry weight (lb) / ((100 - 13% moisture) / 100) Seed weight at 13% moisture was then converted to a yield in bushels per acre (bu / A): (Seed weight (lb) at 13% moisture) x (43,560 Yield (bu / A at 13% =                        ft2 / A) moisture) (plot area (ft2)) x (60 Ib / bu)

[0261] If applicable, plot dimensions recorded in meters were converted to feet and the weight unit of seed was converted to pounds prior to yield calculations. 100-Seed Weight

[0262] 100-seed weight for each plot was determined as follows: Weight of 100 seeds was adjusted to 0% moisture content (100-seed dry weight): 100-seed dry weight (g) = 100-seed fresh weight (g) x [(100 - % actual moisture) / 100] 100-seed dry weight was then adjusted to 13% moisture content: 100-seed weight at 13% moisture (g) = 100-seed dry weight (g) / ((100 - 13% moisture) / 100) Selection of Statistical Method

[0263] The following rules were implemented for each agronomic characteristic: If < 50% of sites had uniform data values for either COR23134 soybean or the control soybean, and < 50% of all data across sites for each entry were at a uniform value, then an across-site mixed model analysis would be conducted. In addition, if both soybean lines had at least two data points at a given site that were not at a uniform value, then an individual-site mixed model analysis would be conducted. If > 50% of sites had uniform data values across both soybean lines, then statistical analyses would not be performed.

[0264] If the criteria described above were not met, then an across-site analysis using the generalized Cochran-Mantel-Haenszel (CMH) test would be conducted. Individual-site analyses would not be performed. Across-Site Analysis Mixed Model Analysis

[0265] For a given agronomic characteristic, data were analyzed using the following linear mixed model: Model 1 yijk = Pi + + n(j) + (pl)ij + Sijk -tj ~ iidN(0, c2site), n(j) ~ iidN(0, (72Rep), (pl)y ~ iidN(0, <j2EntrSite), and syk ~ iidN(0, a2Error), where pt denotes the mean of the zth entry (fixed effect), / / denotes the effect of the jth site (random effect), ny) denotes the effect of the   block within the jth site (random effect), (ptytj denotes the interaction between the entries and sites (random effect), and Eijk denotes the effect of the plot assigned the zth entry in the A111 block of the jth site (random effect or residual). Notation ~ HdN(0, da) indicates random variables that are identically independently distributed (iid) as normal with zero mean and variance o2a. Subscript a represents the corresponding source of variation.

[0266] The residual maximum likelihood estimation procedure was utilized to generate estimates of variance components and entry means across sites. The estimated means are known as empirical best linear unbiased estimators (hereafter referred to as LS-Means). The statistical comparison was conducted by testing for a difference in LS-Means between COR23134 soybean and the control soybean. The approximated degrees of freedom for the statistical test were derived using the Kenward-Roger method (Kenward and Roger, 2009). A significant difference was identified if a P-value was < 0.05.

[0267] For each agronomic characteristic, goodness-of-fit of the model was assessed in terms of meeting distributional assumptions of normally, independently distributed errors with homogeneous variance. Deviations from assumptions were addressed using an appropriate transformation or allowing for heterogeneous error variance among sites. Generalized CMH Test

[0268] The generalized CMH test is more appropriate in the instance where the normality assumption of mixed model analysis cannot be achieved for discrete data. The test was developed specifically for stratified nominal-by-ordinal contingency tables (Agresti, 2002; Koch et al., 1990). It compares entries (a nominal variable) based on their values (recorded on an ordinal scale) while controlling for location (the stratifying variable). Due to the data values being used as the scores in the generalized CMH test, the test’s P-value can be directly interpreted as testing for the difference between the arithmetic means of two entries. A significant difference was identified if a P-value was < 0.05. Individual-Site Analyses

[0269] For a given agronomic characteristic, individual sites were analyzed separately using the following linear mixed model: ytk fk^ Eik Model 2 n ~ iid N(0,<j2ReP) and stk ~ iid N(0, a2 Error), where / / / denotes the mean of the zth entry (fixed effect), n denotes the effect of the   block (random effect), and etk denotes the residual for the observation obtained from the plot assigned to the zth entry in the block.

[0270] The residual maximum likelihood estimation procedure was used to generate estimates of variance components and entry means (LS-Means). The statistical comparison was conducted by testing for difference in LS-Means between diclosulam-treated or untreated COR23134 soybean and the control soybean. The approximated degrees of freedom for the statistical test were derived using the Kenward-Roger method. False Discovery Rate Adjustment

[0271] The false discovery rate (FDR) method (Benjamini and Hochberg, 1995; Westfall et al., 1999) was used to control for false positive outcomes across all agronomic characteristics analyzed using linear mixed models or generalized CMH tests. A false positive outcome occurs if the difference in means between two entries is declared significant, when in fact the two means are not different. Since the introduction of the FDR approach in the mid-1990s, it has been widely employed across a number of scientific disciplines, including genomics, ecology, medicine, plant breeding, epidemiology, dairy science, and signal / image processing (e.g., Pawitan et al., 2005; Spelman and Bovenhuis, 1998). In the FDR method, the false discovery rate is held at 5% across comparisons of multiple agronomic characteristics via an adjustment to the P-value and is not inflated by the number of agronomic characteristics in the comparison. The FDR adjustment of raw P-values was conducted separately for the across-site analysis and each of the individual-site analyses. Statistical Software and Procedures

[0272] Statistical analyses were conducted using SAS software, Version 9.4 (SAS Institute Inc.). SAS PROC MIXED was utilized to fit Models 1 and 2, and to provide LS-Means, 95% confidence intervals, and statistical comparisons. SAS PROC FREQ was used to perform the generalized CMH test. SAS PROC MULTTEST was utilized to provide FDR adjusted P-values. All other data processing was conducted in Base SAS. Interpretation of Statistical Results

[0273] For a given agronomic characteristic, when a statistically significant difference (P-value < 0.05) was identified in the across-site analysis, the respective range of individual values from diclosulam-treated or untreated COR23134 soybean was compared to the instudy reference range comprised of all individual values across-sites from all non-GM reference soybean lines included in this study. In cases when a raw P-value indicated a significant difference but the FDR adjusted P-value was > 0.05, it was concluded that the difference was likely a false positive. In addition, for agronomic characteristics exhibiting a statistically significant difference (P-value < 0.05) in the across-site analysis, the results for individual sites were evaluated. Reported Statistics

[0274] For agronomic characteristics examined using mixed model analysis, the following statistical results were reported: LS-Means, ranges, 95% confidence intervals, FDR-adjusted P-values, and non-adjusted P-values. For agronomic characteristics examined using CMH test, the following statistical results were reported: arithmetic means, ranges, FDR-adjusted P-values, and non-adjusted P-values. For agronomic characteristics which were not statistically analyzed, arithmetic means and ranges were reported. Additionally, the in-study reference range was provided for all agronomic characteristics.

[0275] Note: The lower and / or upper confidence limits might occasionally fall outside the measurement scale (e.g., 1-9) for an agronomic characteristic. In this case, the lower and / or upper limit of the measurement scale was used as the corresponding confidence limit for reporting purposes. Results of Agronomic Characteristic Evaluation untreated COR23134 soybean study:

[0276] A total of 11 agronomic endpoints were included in the assessment: nine were evaluated using mixed model analysis and one was evaluated using the generalized CMH test. The remaining agronomic endpoint (shattering) did not meet criteria for minimum levels of non-uniformity and was therefore not subjected to comparative analyses.

[0277] No statistically significant differences were identified between COR23134 soybean and the control soybean for six agronomic endpoints that went through across-site analysis.

[0278] A statistically significant difference, before FDR-adjustment, between COR23134 soybean and the control soybean was observed in the across-site analysis for harvest seed moisture. The non-significant FDR-adjusted P-value indicates that this difference was likely a false positive. For harvest seed moisture, 47 of 48 values (one value below the lower reference range) for COR23134 soybean were within the reference data range. No statistically significant difference was observed in the individual-site analyses.

[0279] A statistically significant difference, before and after FDR-adjustment, between COR23134 soybean and the control soybean was observed in the across-site analysis for plant height, 100-seed weight, and pod count. For plant height and 100-seed weight, all values for COR23134 soybean were within the reference data range. Additionally, a statistically significant difference was observed in the individual-site analyses at seven and six sites, respectively. For pod count, 46 of 48 values (one value below the lower reference range and one value above the upper reference range) for COR23134 soybean were within the reference data range. Additionally, a statistically significant difference was observed in the individual-site analyses at four sites. Diclosulam-treated soybean:

[0280] A total of 11 agronomic endpoints were included in the assessment: eight were evaluated using mixed model analysis and two were evaluated using the generalized CMH test. The remaining agronomic endpoint (shattering) did not meet criteria for minimum levels of non-uniformity and was therefore not subjected to comparative analyses.

[0281] No statistically significant differences were identified between diclosulam-treated COR23134 soybean and the control soybean for five agronomic endpoints that went through across-site analysis.

[0282] A statistically significant difference, before FDR-adjustment, between diclosulam-treated COR23134 soybean and the control soybean was observed in the across-site analysis for final stand count. The non-significant FDR-adjusted P-value indicates that this difference was likely a false positive. For final stand count, 47 of 48 values (one value above the upper reference range) for diclosulam-treated COR23134 soybean were within the reference data range. No statistically significant difference was observed in the individual-site analyses.

[0283] A statistically significant difference, before and after FDR-adjustment, between diclosulam-treated COR23134 soybean and the control soybean was observed in the across-site analysis for early stand count, plant height, pod count, and 100-seed weight. For early 78 stand count, 47 of 48 values (one value above the upper reference range) for diclosulam-treated COR23134 soybean were within the reference data range. Additionally, a statistically significant difference was observed in the individual-site analyses at one site. For plant height, pod count, and 100-seed weight, all values for diclosulam-treated COR23134 soybean were within the reference data range. Additionally, a statistically significant difference was observed in the individual-site analyses at five sites for each endpoint. Across-Site Agronomic Characteristics Results

[0284] The results for the across-site analysis of agronomic characteristics are provided in Tables 16 and 17. Conclusion

[0285] The results obtained in this study demonstrated that agronomic characteristics of diclosulam-treated or untreated COR23134 soybean were comparable to those of conventional soybean represented by non-GM near-isoline control soybean and non-GM commercial soybean. For untreated COR23134 soybean, a statistically significant difference was identified in pod count, with 46 of 48 observations within the reference range. For diclosulam-treated soybean, a statistically significant difference was identified in early stand count, with 47 of 48 observations within the reference range. Table 16. Across-Site Analysis of Agronomic Characteristics Results Agronomic Reported Control Untreated Reference Data Characteristic Statistics Soybean Soybean Range Mean 27.9 27.3 Range 21.0-42.0 21.4-42.9 Early Stand Count Confidence 25.2 -30.6 24.6-30.0 12 7 - 38 9 (count / m2) Interval Adjusted P-Value — 0.294 P-Value — 0.176 Mean 96.4 90.1 Range 60.6- 113.4 59.6 - 103.0 Confidence 89.4 - 103.3 83.1 - 97.0 Plant Height (cm) Interval 44.8 - 122.8 Adjusted P-Value — 0.000432^ P-Value — <0.0001* Mean 44.1 52.6 Range 22-66 20-113 Confidence 36.3 - 52.0 44.7 - 60.4 Pod Count (count) Interval 24 - 99 Adjusted P-Value 0.0107^ P-Value — 0.00321* Mean 13.3 13.1 Range 0.0 - 100.0 0.0- 100.0 Confidence NA NA Lodging (%) Interval 0.0 - 90.0 Adjusted P-Value — 0.959 P-Value — 0.955 Mean 0.3 0.2 Range 0-4 0-2 Confidence NA NA Shattering (count) Interval 0 - 3 Adjusted P-Value — NA P-Value — NA Mean 49.0 49.3 Range 39-60 39-62 Days to Flowering Confidence 44.7 - 53.3 45.0 - 53.6 37 - 58 (days) Interval Adjusted P-Value 0.567 P-Value — 0.397 Days to Maturity Mean 126.3 126.3 114 - 146 (days) Range 110 - 145 110- 145 Agronomic Characteristic Reported Statistics Control Soybean Untreated COR23134 Soybean Reference Data Range Confidence 119.4 - 133.1 119.4- 133.1 Interval Adjusted P-Value — 0.959 P-Value — 0.949 Final Stand Count (count / m2) Mean Range Confidence Interval Adjusted P-Value P-Value 26.0 19.2-35.0 23.4 -28.6 25.9 17.1 -37.6 23.3 -28.5 0.958 0.958 14.9 - 35.9 Mean 10.5 10.3 Range 7.2- 13.6 6.8 - 13.8 Harvest Seed Confidence 9.2- 11.7 9.1 - 11.6 7.0- 15.3 Moisture (%) Interval Adjusted P-Value — 0.0983 P-Value — 0.0393* Mean 59.7 61.9 Yield (bu / A) Range Confidence 26-92 50.2 -69.2 30-87 52.4-71.4 Interval 17 - 99 Adjusted P-Value 0.228 P-Value — 0.126 Mean 18.4 17.0 Range 11.4-22.5 10.9-21.8 „„„ „ , „T . , . . Confidence 17.1 - 19.8 15.6 - 18.3 100-Seed Weight (g)     T ,    , °            Interval Adjusted P-Value — 0.000419^ P-Value — <0.0001* 9.5-24.2 Note: Not Applicable (NA); mixed model analysis was not performed. * A statistically significant difference (P-value < 0.05) was observed, f Adjusted P-value < 0.05 was observed. Table 17. Across-Site Analysis of Agronomic Characteristics Results Agronomic Reported Control Diclosulam-Treated Reference Data Characteristic Statistics Soybean COR23134 Soybean Range Mean 27.9 26.6 Range 21.0-42.0 18.9-40.2 Early Stand Count (count / m2) Confidence Interval 25.2 -30.6 23.9-29.3 12.7 - 38.9 Adjusted P-Value — 0.00890^ P-Value — 0.00356* Mean 96.4 91.3 Range 60.6- 113.4 58.6 - 104.2 Plant Height (cm) Confidence Interval 89.5 - 103.4 84.3 -98.2 44.8 - 122.8 Adjusted P-Value — 0.00349^ P-Value — 0.000697* Mean 44.1 52.8 Range 22-66 32-88 Pod Count (count) Confidence Interval 36.3 - 52.0 44.9-60.6 24 - 99 Adjusted P-Value — 0.00665^ P-Value — 0.00266* Mean 13.3 13.4 Range 0.0 - 100.0 0.0- 100.0 Lodging (%) Confidence Interval NA NA 0.0-90.0 Adjusted P-Value — 0.976 P-Value — 0.976 Mean 0.3 0.3 Range 0-4 0- 1 Shattering (count) Confidence Interval NA NA 0 - 3 Adjusted P-Value — NA P-Value — NA Mean 49.0 48.9 Range 39-60 38-62 Days to Flowering (days) Confidence Interval 44.7 - 53.3 44.6- 53.2 37 - 58 Adjusted P-Value 0.950 P-Value — 0.855 Mean 126.3 126.2 114 - 146 Agronomic Characteristic Reported Statistics Control Soybean Diclosulam-Treated COR23134 Soybean Reference Data Range Days to Maturity (days) Range Confidence Interval Adjusted P-Value P-Value 110-145 119.4 - 133.1 111-145 NA 0.934 0.747 Final Stand Count (count / m2) Mean Range Confidence Interval Adjusted P-Value P-Value 26.0 19.0-35.0 23.4 -28.6 25.1 18.0-38.0 22.5 -27.7 0.0623 0.0311* 15.0 - 36.0 Final Stand Count (count / m2) Mean Range Confidence Interval Adjusted P-Value P-Value 26.0 19.2-35.0 23.4 -28.6 25.1 17.9-38.5 22.5 -27.7 0.0606 0.0303* 14.9 - 35.9 Harvest Seed Moisture (%) Mean Range Confidence Interval Adjusted P-Value P-Value 10.5 7.2- 13.6 9.2- 11.7 10.4 6.9 - 13.6 9.1 - 11.6 0.254 0.178 7.0- 15.3 Yield (bu / A) Mean Range Confidence Interval Adjusted P-Value P-Value 59.7 26-92 50.2 -69.2 61.9 29-84 52.4-71.4 0.210 0.126 17 - 99 Mean 18.4 17.0 Range 11.4-22.5 11.2-22.3 ,    _ , „T . , . . Confidence 17.1 - 19.8 15.7 - 18.4 100-Seed Weight (g)     : ,    , °            Interval 9.5-24.2 Adjusted P-Value — 0.000627^ P-Value — <0.0001* Note: Herbicide-treated refers to treatment with diclosulam. Not applicable (NA); mixed model analysis was not performed. * A statistically significant difference (P-value < 0.05) was observed. f Adjusted P-value < 0.05 was observed. Example 7. Protein Expression and Concentration

[0286] COR-23134-4 soybean plants were grown during the 2022 growing season at six sites in commercial soybean-growing regions of the United States (one site in Iowa, Illinois, Indiana, Nebraska, and Pennsylvania) and Canada (one site in Ontario). A randomized complete block design with four blocks was utilized at each site. Each block included COR-23134-4 soybean and herbicide-treated COR-23134-4 soybean.

[0287] The following samples were collected and processed for each entry: leaf (V5, RI, and R3 growth stages), flowers (R1-R2 growth stage), root (R3 growth stage), forage (R3 growth stage), and seed (R8 growth stage). Growth stage descriptions are provided in Table 18. Samples were analyzed for CrylB.34.1, CrylB.61.1, IPD083Cb, and GM-HRA protein concentrations using quantitative enzyme-linked immunosorbent assay (ELISA) methods.

[0288] Control of bias for expressed trait protein analysis was achieved through the use of replicate testing, appropriate assay controls, and pre-set data acceptability criteria. Sample Collection and Processing

[0289] Leaf (V5, RI, and R3 growth stages), flowers (R1-R2 growth stage), root (R3 growth stage), forage (R3 growth stage), and seed (R8 growth stage) samples were collected, lyophilized, homogenized, and stored frozen. Protein Concentration Determination

[0290] The concentrations of Cry IB.34.1, CrylB.61.1, IPD083Cb, and GM-HRA proteins were determined using quantitative ELISA methods that have been internally validated to demonstrate method suitability. The number of samples analyzed is provided in Table 19. Protein Extraction

[0291] Processed tissue sub-samples were weighed at the following target weights: 5 mg for flowers, 10 mg for leaf and seed, and 20 mg for root and forage. Sub-samples were stored in a -20 °C freezer unit until analysis.

[0292] Each sample analyzed for CrylB.34.1, CrylB.61.1, and IPD083Cb was extracted with 0.60 ml of chilled phosphate-buffered saline containing 0.05% polysorbate 20 (PBST). Each leaf, root, forage, and seed sample analyzed for GM-HRA was extracted with 0.60 ml of chilled buffer which was comprised of PBST with 0.2% CHAPS. Flower samples analyzed for GM-HRA were extracted with 0.60 ml of chilled buffer comprised of PBST with 0.2% CHAPS and 5% StabilZyme Select. Extracted samples were centrifuged, and then supernatants were removed and prepared for analysis. CrylB.34.1 Protein ELISA Method

[0293] Prior to analysis, samples were diluted as applicable in PBST. Standards (typically analyzed in triplicate wells) and diluted samples (typically analyzed in duplicate wells) were incubated in a plate pre-coated with a monoclonal antibody. Following incubation, unbound substances were washed from the plate. A different monoclonal antibody conjugated to the enzyme horseradish peroxidase (HRP) was added to the plate and incubated. Unbound substances were washed from the plate. Detection of the bound Cry IB.34.1-antibody complex was accomplished by the addition of substrate, which generated a colored product in the presence of HRP. The reaction was stopped with an acid solution and the optical density (OD) of each well was determined using a plate reader. CrylB.61.1 Protein ELISA Method

[0294] Prior to analysis, samples were diluted as applicable in PBST. Standards (typically analyzed in triplicate wells) and diluted samples (typically analyzed in duplicate wells) were incubated in a plate pre-coated with a monoclonal antibody. Following incubation, unbound substances were washed from the plate. A different monoclonal antibody conjugated to the enzyme HRP was added to the plate and incubated. Unbound substances were washed from the plate. Detection of the bound CrylB.61.1-antibody complex was accomplished by the addition of substrate, which generated a colored product in the presence of HRP. The reaction was stopped with an acid solution and the OD of each well was determined using a plate reader. IPD083Cb Protein ELISA Method

[0295] Prior to analysis, samples were diluted as applicable in PBST. Standards (typically analyzed in triplicate wells) and diluted samples (typically analyzed in duplicate wells) were incubated in a plate pre-coated with a monoclonal antibody. Following incubation, unbound substances were washed from the plate. A different monoclonal antibody conjugated to the enzyme HRP was added to the plate and incubated. Unbound substances were washed from the plate. Detection of the bound IPD083Cb-antibody complex was accomplished by the 85 addition of substrate, which generated a colored product in the presence of HRP. The reaction was stopped with an acid solution and the OD of each well was determined using a plate reader. GM-HRA Protein ELISA Method

[0296] Prior to analysis, samples were diluted as applicable in PBST containing 0.2% CHAPS and 5% StabilZyme Select. Standards (typically analyzed in triplicate wells) and diluted samples (typically analyzed in duplicate wells) were incubated in a plate pre-coated with a polyclonal antibody. Following incubation, unbound substances were washed from the plate and the bound GM-HRA protein was incubated with a monoclonal antibody, conjugated to the enzyme HRP. Unbound substances were washed from the plate. Detection of the bound GM-HRA-antibody complex was accomplished by the addition of substrate, which generated a colored product in the presence of HRP. The reaction was stopped with an acid solution and the OD of each well was determined using a plate reader. Calculations for Determining CrylB.34.1, CrylB.61.1, IPD083Cb, and GM-HRA Protein Concentrations

[0297] SoftMax Pro GxP Version 7.0.3 (Molecular Devices) microplate data software was used to perform the calculations required to convert the OD values obtained for each set of sample wells to a protein concentration value.

[0298] A standard curve was included on each ELISA plate. The equation for the standard curve was derived by the software, which used a quadratic fit to relate the OD values obtained for each set of standard wells to the respective standard concentration (ng / ml).

[0299] The sample concentration values were adjusted for a dilution factor expressed as 1 :N by multiplying the interpolated concentration by N. Adjusted Concentration = Interpolated Sample Concentration x Dilution Factor

[0300] Adjusted sample concentration values obtained from SoftMax Pro GxP software were converted from ng / ml to ng / mg sample weight as follows: Sample Concentration            Sample (ng protein / mg sample = Concentration x weight)                           (ng / ml) Extraction Buffer Volume ___________(ml)___________ Sample Target Weight (mg)

[0301] The reportable assay lower limit of quantification (LLOQ) in ng / ml was calculated as follows: Reportable Assay LLOQ (ng / ml) = (lowest standard concentration - 10%) x minimum dilution The LLOQ, in ng / mg sample weight, was calculated as follows: Extraction Buffer Volume T T         Reportable Assay LLOQ                    / n LLOQ = r / / n           x ___________LIEU__________ m '                Sample Target Weight (mg) Trait Confirmation

[0302] To confirm sample identity, event-specific polymerase chain reaction (PCR) analyses were performed for samples with unexpected ELISA results. If a given test sample was confirmed as not containing the event of interest, the protein results were excluded from reporting. Statistical Analysis

[0303] Statistical analysis of the protein concentration results consisted of the calculations of means, ranges, and standard deviations. Individual sample results below the LLOQ were assigned a value equal to the LLOQ for calculation purposes. Results

[0304] The concentration results for the Cry IB.34.1, Cry IB.61.1, IPD083Cb, and GM-HRA proteins are provided in Tables 20, 21, 22, and 23, respectively. Conclusion

[0305] Cry IB.34.1, Cry IB.61.1, IPD083Cb, and GM-HRA protein concentration results for COR-23134-4 soybean and herbicide-treated COR-23134-4 soybean are summarized across sites as means, ranges, and standard deviations. Table 18. Soybean Growth Stage Descriptions Growth Stage Description VE Emergence - Occurs when plants first emerge from the soil. VC Cotyledon - Occurs when the unifoliate leaves have unfolded. VI First Node - Occurs when the leaflets on the second leaf node have unrolled. V2 Second Node - Occurs when the leaflets on the third leaf node have unrolled. V3 Third Node - Occurs when the leaflets on the fourth leaf node have unrolled. V4 Fourth Node - Occurs when the leaflets on the fifth leaf node have unrolled. V5 Fifth Node - Occurs when the leaflets on the sixth leaf node have unrolled. RI Beginning Bloom - Occurs when plants have one open flower at any node on the main stem. R2 Full Bloom - Occurs when plants have an open flower at one of the two uppermost nodes. R3 Beginning Pod - Occurs when a pod is 5 millimeters long at one of the four uppermost nodes. R4 Full Pod - Occurs when a pod is 2 centimeters long at one of the four uppermost nodes. R5 Beginning Seed - Occurs when a pod at one of the four uppermost nodes has seed 3 millimeters long. R6 Full Seed - Occurs when a pod is filled to capacity with green seed at one of the four uppermost nodes. R7 Beginning Maturity - Occurs when one pod on the main stem has reached mature pod color. R8 Full Maturity - Occurs when 95% of the pods have reached their mature pod color. Note: Growth stages (Pedersen, 2004). Table 19. Summary of Soybean Samples Analyzed Soybean Line Tissue (Growth Stage) Number of Samples Analyzed IA5 IL7 IN2 NE1 ON2 PAI Total COR-23134-4 Soybean Leaf (V5) 4 4 4 4 4 4 24 Leaf (RI) 4 4 4 4 4 4 24 Leaf (R3) 4 4 4 4 4 4 24 Flowers (RI -R2) 4 4 4 4 4 4 24 Root (R3) 4 4 4 4 4 4 24 Forage (R3) 4 4 4 4 4 4 24 Seed (R8) 4 4 4 4 4 4 24 Herbicide- Treated COR-23134-4 Soybean Leaf (V5) 4 4 4 4 4 4 24 Leaf (RI) 4 4 4 4 4 4 24 Leaf (R3) 4 4 4 4 4 4 24 Flowers (RI -R2) 4 4 4 4 4 4 24 Root (R3) 4 4 4 4 4 4 24 Forage (R3) 4 4 4 4 4 4 24 Seed (R8) 4 4 4 4 4 4 24 Note: Growth stages (Pedersen, 2004). Herbicide-treated refers to treatment with diclosulam. Table 20. Across-Site Summary of Expressed Trait CrylB.34.1 Protein Concentrations Tissue (Growth Stage) ng CrylB.34.1 / mg Tissue Dry Weight Number of Samples <LLOQ / Number of Samples Reported Mean Range Standard Deviation Sample LLOQa COR-23134-4 Soybean Leaf (V5) 460 190 - 960 220 0.14 0 / 24 Leaf (RI) 310 150 -780 170 0.14 0 / 24 Leaf (R3) 170 84 - 270 44 0.14 0 / 24 Flowers (RI - R2) 260 200 - 320 28 0.28 0 / 24 Root (R3) 77 15 - 170 39 0.069 0 / 24 Forage (R3) 150 75 - 180 22 0.069 0 / 23b Seed (R8) 170 140-210 22 0.14 0 / 24 Herbicide-Treated COR-23134-4 Soybean Leaf (V5) 440 180 - 840 220 0.14 0 / 24 Leaf (RI) 300 160 - 660 150 0.14 0 / 24 Leaf (R3) 210 110 - 590 110 0.14 0 / 24 Flowers (RI - R2) 250 180-310 46 0.28 0 / 24 Root (R3) 82 23-210 40 0.069 0 / 24 Forage (R3) 140 84 - 170 22 0.069 0 / 24 Seed (R8) 160 110-210 24 0.14 0 / 24 4ote: Growth stages (Pedersen, 2004). Herbicide-treated refers to treatment with diclosulam. a Lower limit of quantification (LLOQ) in ng / mg tissue dry weight. b One forage sample was confirmed negative for the event of interest by polymerase chain reaction (PCR) analysis. Table 21. Across-Site Summary of Expressed Trait CrylB.61.1 Protein Concentrations Tissue (Growth Stage) ng CrylB.61.1 / mg Tissue Dry Weight Number of Samples <LLOQ / Number of Samples Reported Mean Range Standard Deviation Sample LLOQa COR-23134-4 Soybean Leaf (V5) 400 160 - 720 120 0.28 0 / 24 Leaf (RI) 490 250- 1300 220 0.28 0 / 24 Leaf (R3) 480 260 - 780 130 0.28 0 / 24 Flowers (RI - R2) 150 120 - 190 19 0.55 0 / 24 Root (R3) 0.34b <0.14-0.93 0.18b 0.14 1 / 24 Forage (R3) 200 69 - 390 72 0.14 0 / 23c Seed (R8) 15 11-22 2.6 0.28 0 / 24 Herbicide-Treated COR-23134-4 Soybean Leaf (V5) 450 270 - 900 150 0.28 0 / 24 Leaf (RI) 460 260 - 720 110 0.28 0 / 24 Leaf (R3) 460 250 - 660 130 0.28 0 / 24 Flowers (RI - R2) 140 100 - 180 23 0.55 0 / 24 Root (R3) 0.33b <0.14-0.87 0.17b 0.14 3 / 24 Forage (R3) 160 63 - 220 44 0.14 0 / 24 Seed (R8) 17 12-20 2.4 0.28 0 / 24 4ote: Growth stages (Pedersen, 2004). Herbicide-treated refers to treatment with diclosulam. a Lower limit of quantification (LLOQ) in ng / mg tissue dry weight. b Some, but not all, sample results were below the LLOQ. A value equal to the LLOQ value was assigned to those samples to calculate the mean and standard deviation. c One forage sample was confirmed negative for the event of interest by polymerase chain reaction (PCR) analysis. Table 22. Across-Site Summary of Expressed Trait IPD083Cb Protein Concentrations Tissue (Growth Stage) ng IPD083Cb / mg Tissue Dry Weight Number of Samples <LLOQ / Number of Samples Reported Mean Range Standard Deviation Sample LLOQa COR-23134-4 Soybean Leaf (V5) 65 48 - 110 17 1.2 0 / 24 Leaf (RI) 71 48-96 14 1.2 0 / 24 Leaf (R3) 83 59- 130 19 1.2 0 / 24 Flowers (Rl- R2) 69 56 - 85 8.7 2.4 0 / 24 Root (R3) 21 12-33 5.6 0.60 0 / 24 Forage (R3) 59 39 - 84 10 0.60 0 / 23b Seed (R8) 14 13 - 18 1.4 1.2 0 / 24 Herbicide-Treated COR-23134-4 Soybean Leaf (V5) 62 40 - 84 14 1.2 0 / 24 Leaf (RI) 76 56- 100 14 1.2 0 / 24 Leaf (R3) 77 47-110 16 1.2 0 / 24 Flowers (Rl- R2) 64 44 - 88 13 2.4 0 / 24 Root (R3) 19 3.0-33 6.2 0.60 0 / 24 Forage (R3) 59 36 - 84 12 0.60 0 / 24 Seed (R8) 15 13 - 19 1.5 1.2 0 / 24 4ote: Growth stages (Pedersen, 2004). Herbicide-treated refers to treatment with diclosulam. a Lower limit of quantification (LLOQ) in ng / mg tissue dry weight. b One forage sample was confirmed negative for the event of interest by polymerase chain reaction (PCR) analysis. Table 23. Across-Site Summary of Expressed Trait GM-HRA Protein Concentrations Tissue (Growth Stage) ng GM-HRA / mg Tissue Dry Weight Number of Samples <LLOQ / Number of Samples Reported Mean Range Standard Deviation Sample LLOQa COR-23134-4 Soybean Leaf (V5) 7.6b <2.2- 17 5.0b 2.2 1 / 24 Leaf (RI) 4.4b <2.2- 11 2.6b 2.2 5 / 24 Leaf (R3) 2.5b <2.2-4.4 0.58b 2.2 15 / 24 Flowers (RI - R2) 2.3 1.4-2.9 0.37 1.1 0 / 24 Root (R3) 1.0 0.60- 1.5 0.25 0.27 0 / 24 Forage (R3) 1.4b <1.1 -2.2 0.29b 1.1 3 / 23c Seed (R8) 0.73b <0.54 - 1.7 0.28b 0.54 8 / 24 Herbicide-Treated COR-23134-4 Soybean Leaf (V5) 10 2.5-20 5.7 2.2 0 / 24 Leaf (RI) 4.9b <2.2 - 12 3.0b 2.2 5 / 24 Leaf (R3) 3.1b <2.2-6.6 1.3b 2.2 12 / 24 Flowers (RI - R2) 2.2 1.8-2.9 0.33 1.1 0 / 24 Root (R3) 1.0 0.48 - 1.5 0.26 0.27 0 / 24 Forage (R3) 1.2b <1.1 -2.0 0.23b 1.1 12 / 24 Seed (R8) 0.75b <0.54 - 1.3 0.20b 0.54 7 / 24 4ote: Growth stages (Pedersen, 2004). Herbicide-treated refers to treatment with diclosulam. a Lower limit of quantification (LLOQ) in ng / mg tissue dry weight. b Some, but not all, sample results were below the LLOQ. A value equal to the LLOQ value was assigned to those samples to calculate the mean and standard deviation. c One forage sample was confirmed negative for the event of interest by polymerase chain reaction (PCR) analysis. Example 8. Sequence Characterization of Insert and Flanking Genomic Regions of COR-23134-4 Soybean

[0306] Sequence characterization analysis was performed to determine the DNA sequence of the COR23134 insert and flanking genomic regions.

[0307] The sequence of the insert and its flanking genomic regions was determined to confirm the integrity of the inserted DNA in COR23134 soybean. The gDNA extracted from COR23134 soybean plants was used for PCR amplification. Seven overlapping PCR fragments, spanning the insert and flanking genomic regions, were each amplified in two independent reactions. PCR products at the expected size were amplified only from the gDNA of COR23134 soybean but not from the non-GM control soybean or the no-template control. The PCR products were cloned, and at least six plasmids (three from each of the two independent PCR reactions) for each PCR fragment were sequenced in both forward and reverse directions using Sanger sequencing to cover every nucleotide, and the resulting sequencing reads were used to determine the consensus sequence for each PCR fragment. The consensus sequences from all seven overlapping PCR fragments were combined to determine the sequence for COR23134 soybean. The total length of sequence determined for COR23134 soybean is 30,019 base pairs (bp), composed of 1,024 bp of 5' flanking genomic sequence, 1,425 bp of 3' flanking genomic sequence, and 27,570 bp of inserted DNA. In comparison with the sequence of the PHP90315 T-DNA, the COR23134 insert is composed of bps 26 to 27,616 of the PHP90315 T-DNA, except for a 21-bp deletion in the pv-ubi2 promoter at bps 21,719-21,739 relative to the PHP90315 T-DNA sequence. All remaining sequence is intact and identical to the PHP90315 T-DNA sequence.

[0308] The junction sequences of Soybean Event COR-23134-4 are listed in Table 24 below: Table 24. Junction Sequences SEQ ID NO: Description Sequence 12 COR-23134-4 5'end junction (-5 to +5) tttgtgccaa 13 COR-23134-4 5'end junction (-10 to +10) ttatatttgtgccaacacca 14 COR-23134-4 5'end junction (-25 to +25) atgtccatcggatagttatatttgtgccaacaccaaaacatcaaaaaaat 15 COR-23134-4 3'end junction (-5 to +5) cgacattaag 16 COR-23134-4 3'end junction (-10 to +10) aatttcgacattaaggaata 17 COR-23134-4 3' end junction (-25 to +25) aaccttcatatataaaatttcgacattaaggaatacttttgattaaatgc Example 9: Modification of COR-23134-4 event using genome editing

[0001] In this Example, methods to modify the polynucleotide sequence of COR-23134-4 and / or surrounding genomic polynucleotide sequences (SEQ ID NO:3) using genome editing are described.

[0002] In one method, one or more genome editing reagents (for example but not limited to a recombinase (RE) (Wang etal. (2010) Plant Cell Rep. 30:267-285 and Van Duyne (2015) Microbiol Spectr. 3:10.1128 / microbiolspec.mdna3-0014-2014), zinc finger nuclease (ZFN) (Umov et al. (2010) Nat Rev Genet. 11:636-646), transcription activator-like effector nuclease (TALEN) (Joung and Sander (2013) Nat Rev Mol Cell Biol. 14:49-55), homing endonuclease (HE) (Belfort and Bonocora (2014) Methods Mol Biol. 1123:1-26 and Stoddard (2014) Mobile DNA. 5:7), clustered regularly interspaced short palindromic repeat (CRISPR) associated (Cas) effector nuclease (Cong et al. (2013) Science. 339:819-823, Zetsche et al. (2015) Cell. 163:759-771, Yaneta / . (2018) Science. 363:88-91, Pausch eta / . (2020) Science. 369:333-337, Karvelis etal. (2020) Nucleic Acids Res. 48:5016-5023, Yoshimi and Mashimo (2022) Gen and Genome Editing. 3-4:100013, and Urbaitis et al. (2022) EMBO Rep. 23:e55481), transposase associated B (TnpB) nuclease (Karvelis et al. (2021) Nature. 599:692-696 and Altae-Tran et al. (2021) Science. 374:57-65), Fanzor (Saito et al. (2023) Nature. 620:660-668 and Jiang et al. (2023) Sci. Adv. 9:eadk0171), and / or hydrolytic endonucleolytic ribozyme (HYER) (Liu et al. (2024) Science. 383:eadh4859)) are used to excise all or a portion of the polynucleotide sequence of COR-23134-4. In the case of a RE, a target site can be placed (either before or after the transgene or cisgene is inserted into the genome) flanking the polynucleotide region to be excised (FIG. 4). If the target site is included before insertion into the genome, it can be incorporated into the trait DNA sequence by first synthesizing a DNA fragment containing the site (Integrated DNA Technologies, GenScript, and / or Twist Bioscience) and then inserted using a NEBuilder HiFi DNA Assembly kit per the manufacturer’s instruction (New England Biolabs) although other vector construction methods can be used (for example but not limited to restriction enzyme, Golden Gate, and Gateway cloning). If the target site is incorporated following genomic insertion, it can be introduced using standard genome editing methodologies which include prime editing 95 (Anzalone et al. (2019) Nature. 576:149-157) or non-homologous end-joining (NHEJ), micro-homology mediated end-joining (MMEJ), and / or homologous recombination (HR) repair of a targeted DNA nick or double-strand break (DSB) in the presence of a DNA repair template containing the target site of interest (Yao et al. (2017) Cell Research. 27:801-814). Once introduced, the recombinase can be delivered (for example but not limited to particle bombardment (Ozyigit and Kurtoglu (2020) Mol Biol Rep. 47:9831-9847) or Agrobacterium transformation (Sardesai and Subramanyam (2018) In: Gelvin SB (ed) Agrobacterium Biology: From Basic Science to Biotechnology. Cham: Springer International Publishing, 463-488)) either transiently or from a DNA expression cassette in a constitutive or tissuespecific manner. Once present in the cell, the RE can recognize its target site(s) and through strand exchange loop-out the intervening sequence (FIG. 4) (Dale and Ow (1991) Proc Natl AcadSci USA. 88:10558-10562 and Russell et al. (1992) Mol Gen Genet. 234:49-59).

[0003] Since ZFNs, TALENs, Cas endonucleases, TnpBs, Fanzors, and / or HYER nucleases can be programmed to recognize new targets, one or more target sequences in COR-23134-4 or its surrounding sequence can be selected and the respective endonuclease(s) engineered to cleave the new site(s). Next, the reprogrammed nuclease can be delivered using particle bombardment or Agrobacterium as described above. Then upon delivery, the DNA editing reagent can be directed to recognize and cut its DNA target(s). The sequence between recognition site(s) can then be removed by leveraging cellular repair of the resulting DNA double-strand breaks (DSBs) (for example but not limited to non-homologous end-joining (NHEJ), micro-mediated end-joining (MMEJ), single-strand annealing (SSA), or homologous recombination (HR) (Gao et al. (2020) Nat Biotech. 38:579-581 and Sfeir and Symington (2015) Trends Biochem Sci. 40:701-714)) (FIG. 5). In the instance of Type I Cas effector complexes, one or more targets can be selected in the vicinity of COR-23134-4 and then once delivered transiently or expressed in a tissue-specific or constitutive manner used to processively remove sequences adjacent to the target site(s) optionally in combination with an orthogonal nuclease inactive enzyme (FIG. 6) (Dolan et al. (2019) Mol Cell. 74:936-950 and Li et al. (2024) Sci Adv. 10:eadk8052).

[0004] In a second method, one or more genome editing reagents (for example but not limited to a RE, ZFN, TALEN, HE, Cas effector nuclease, TnpB nuclease, Fanzor, and / or HYER nuclease) are used to introduce polynucleotide sequence variation in or in the vicinity of COR-23134-4. For this, one or more target sites are first selected and then a reprogrammed RE, ZFN, TALEN, HE, Cas effector nuclease, TnpB nuclease, Fanzor, and / or HYER nuclease can be used to generate one or more DSBs. Cellular repair of the resulting DSB(s) by NHEJ, MMEJ, SSA, or HR can then be used to introduce targeted sequence variation in the promoter(s), intron(s), coding sequence(s) (CDS), terminator(s), and / or flanking sequence of COR-23134-4 to knock-out or disrupt gene expression, up- or down-regulate gene expression, modify the coding content, and / or alter the flanking genomic polynucleotides (FIG. 7). Additionally, an adenine or cytosine deaminase and / or glycosylase can be recruited to the Cas, TnpB, and / or Fanzor gRNA complex and used to introduce cytosine to thymine, adenine to guanine, and other transition or inversion single polynucleotide polymorphisms (SNPs) in COR-23134-4 (Komor et al. (2016) Nature. 533:420-424, Nishida et al. (2016) Science. 353: aaf8729, Gaudelli et al. (2017) Nature. 551:464-471, Tong et al. (2023) National Science Review. 10: nwadl43, and Yi et al. (2024) Nat Commun. 15:6397). Moreover, prime editing can be utilized to introduce SNPs, insertions, deletions, and combinations thereof into Event COR-23134-4 (Chen and Liu (2022) Nat Rev Genet. 24:161177). Similar to that described above, this can be used to disrupt or knock-out gene expression, up- or down-regulate gene expression, and / or generate variation in the coding content of one or more genes encoded in COR-23134-4 (FIG. 7). For a RE, a target (for example but not limited to a loxP site) can be placed (either before or after genomic insertion) in opposing orientations and, then once recognized by the RE, the polynucleotide sequence between the sites inverted (FIG. 8). This can be used to modulate gene expression turning it either on or off or used to switch between constitutive and tissue-specific expression or vice versa. Moreover, a RE can be used to perform recombinase mediated cassette exchange (RMCE) to swap-out one or more genetic elements (for examples but not limited to promoter(s), intron(s), CDS(s), and terminator(s)) with different ones (Gao etal. (2020) Front Plant Sci. 11:535).

[0005] Table 25 lists polynucleotide target sites (comprised of a guide RNA recognition site (gRNArs) and 3’ NGG target or protospacer adjacent motif (TAM or PAM) where N can be either a cytosine, adenine, thymine, or guanine polynucleotide) for a Cas9 (SEQ ID NO: 45) that can be used to excise all or a portion of COR-23134-4 or introduce targeted polynucleotide sequence variation in COR-23134-4. Target sites were initially selected by first identifying an appropriate PAM in the insert and flanking regions on either DNA strand of COR-23134-4 (SEQ ID NO:3). Next, the 16-30 nts immediately 5’ of the PAM were selected and off-target sites predicted using Cas-OFFinder (Bae et al. (2014) Bioinformatics. 30:1473-1475) against the Williams 82 soybean genomic reference sequence (Garg et al. (2023) Plant Genome. 16:e20382). Unique targets, defined as having at least two nucleotide differences from the closest sequence in the Williams 82 genome and less than or equal to 3 consecutive adenine, thymine, guanine, and cytosine nts in the gRNArs, can then be prioritized for COR-23134-4 editing. To modulate the expression of genes within COR-23134-4, targets can be further selected within the regulatory regions of the respective gene (for example but not limited to a TATA box, initiator element, downstream promoter element, TFIIB recognition element, downstream core element, motif ten element, X core promoter element, intron, 5’ untranslated region (UTR), and / or 3’ UTR). In some instances, this can be completed using a neural network trained for prediction of gene expression in plants (Peleke et al. (2024) Nat Commun. 15:3488). To improve the fitness and / or efficacy of proteins encoded within COR-23134-4 (for example but not limited to Cry IB.34.1 (SEQ ID NO:5), Cry IB.61.1 (SEQ ID NO:7), and IPD083 Cb (SEQ ID NO:9)), in silica evolution can be performed and various protein models (for example but not limited to data on efficacy and / or toxicity and / or ProteinMPNN (Dauparas et al. (2022) Science. 378:49-56)) used to rank variants. Next, depending on how many in silica changes were introduced, an editing strategy can be devised (for example but not limited to homologous recombination, prime editing, and / or base editing) and targets selected. Table 25. Cas9 target sites to facilitate the removal of all or a portion of COR-23134-4 or introduce targeted polynucleotide sequence variation in elements of COR-23134-4. Target Site Name Cas9 Target Site (gRNArs+PAM) SEQ ID NO: COR-23134-4 Target 1 GGTTGATTATTAGGACTAGGGGG 46 COR-23134-4 Target 2 GATTCAGTGAAGTTGTGTGGGGG 47 COR-23134-4 Target 3 GCAATCAACATGTATTAGTGAGG 48 COR-23134-4 Target 4 GACTCTGAGGAGATATCTATTGG 49 COR-23134-4 Targets GATTCAACCGTTTGCATCCGCGG 50 COR-23134-4 Target 6 GTTCCAATTTATAGGGAAACCGG 51 COR-23134-4 Target 7 GAAGCGATACGAGTTTAGAGGGG 52 COR-23134-4 Targets GGAATCTAGGATTTGGTAGAGGG 53 COR-23134-4 Target 9 GAACCACGGAATCTAGGATTTGG 54 COR-23134-4 Target 10 GGGCCTTGTGCTGACTGAGATGG 55 COR-23134-4 Target 11 GAATCGGGTGGTTCTGGAAGCGG 56 COR-23134-4 Target 12 GAGCGTGGTTGGGTTTGGTGAGG 57 COR-23134-4 Target 13 GTGAAGGGCGCCGCCGTGGGGGG 58 COR-23134-4 Target 14 GGTGAAGGGCGCCGCCGTGGGGG 59 COR-23134-4 Target 15 GTCGGCGCTTCCTTGGTGAAGGG 60 COR-23134-4 Target 16 GGTCGGCGCTTCCTTGGTGAAGG 61 COR-23134-4 Target 17 GACACGAAGGGCTCCGTGGTCGG 62 COR-23134-4 Target 18 GCCCTTGCGAGGTTCGCCGGAGG 63 COR-23134-4 Target 19 GCCTCCGGCGAACCTCGCAAGGG 64 COR-23134-4 Target 20 GGAGAGGCAGGGCGTGACGACGG 65 COR-23134-4 Target 21 GATCTGCTCACCTCCACGATCGG 66 COR-23134-4 Target 22 GAAAGCCTCGGCGACGACGCGGG 67 COR-23134-4 Target 23 GTTCCAATTGGGCCTCGGCGGGG 68 COR-23134-4 Target 24 GTGCTGCTGTTGCTAACCCTGGG 69 COR-23134-4 Target 25 GAGATTCTCCACTCTTATAGTGG 70 COR-23134-4 Target 26 GGAGTTGGCCACTATAAGAGTGG 71 COR-23134-4 Target 27 GATGATCCTAATACTATGGTAGG 72 COR-23134-4 Target 28 ACTCTGCATAAATCTGGTTTGGG 73 COR-23134-4 Target 29 TACTCTGCATAAATCTGGTTTGG 74 COR-23134-4 Target 30 TTCCTTACTCTGCATAAATCTGG 75 COR-23134-4 Target 31 AACCAGATTTATGCAGAGTAAGG 76 COR-23134-4 Target 32 ACAGAGCTGTGTCCGAATAATGG 77 COR-23134-4 Target 33 AGGTTGATTATTAGGACTAGGGG 78 COR-23134-4 Target 34 AAGGTTGATTATTAGGACTAGGG 79 COR-23134-4 Target 35 AATCATAAAGGTTGATTATTAGG 80 COR-23134-4 Target 36 ACACAGTATGATAATCATAAAGG 81 COR-23134-4 Target 37 AAAGGAAACCTGTATGGTATGGG 82 COR-23134-4 Target 38 AAGAAGTACCCATACCATACAGG 83 COR-23134-4 Target 39 CTAGTGTTGTAGTTAGTGCTTGG 84 COR-23134-4 Target 40 CCTGAACTTGGATTTGATTTTGG 85 COR-23134-4 Target 41 TTTGGTATATGACCTGAACTTGG 86 COR-23134-4 Target 42 ACATTAGACCATTCATGTTAAGG 87 COR-23134-4 Target 43 AAGTTGCCTCAAATTCCAATAGG 88 COR-23134-4 Target 44 AGTTGCCTCAAATTCCAATAGGG 89 COR-23134-4 Target 45 CTTAAGGGCATAGGACGAGTTGG 90 COR-23134-4 Target 46 ACATATAAGAAATGCCTTAAGGG 91 COR-23134-4 Target 47 TACATATAAGAAATGCCTTAAGG 92 COR-23134-4 Target 48 CAACTCGTCCTATGCCCTTAAGG 93 COR-23134-4 Target 49 AAGATACTCAAGTAAATTCTAGG 94 COR-23134-4 Target 50 TCGTGAAAGAGAATCTGTTAAGG 95 COR-23134-4 Target 51 GCACAAATATAACTATCCGATGG 96 COR-23134-4 Target 52 AGTTGTGTTAGAATGTCCATCGG 97 COR-23134-4 Target 53 AGTGATTCAGTGAAGTTGTGTGG 98 COR-23134-4 Target 54 TTATTTATATGGGCTTCAAGTGG 99 COR-23134-4 Target 55 TTATATGGGCTTCAAGTGGTTGG 100 COR-23134-4 Target 56 TATATGGGCTTCAAGTGGTTGGG 101 COR-23134-4 Target 57 ATATGGGCTTCAAGTGGTTGGGG 102 COR-23134-4 Target 58 AAGTTAACAAACATATCTACTGG 103 COR-23134-4 Target 59 TTTCCACGTCACCTCCTCAATGG 104 COR-23134-4 Target 60 TTTCTTATGCTATTCCATTGAGG 105 COR-23134-4 Target 61 CTTATGCTATTCCATTGAGGAGG 106 COR-23134-4 Target 62 ATTCCATTGAGGAGGTGACGTGG 107 COR-23134-4 Target 63 TATCTCAGACATTGAAACTAAGG 108 COR-23134-4 Target 64 CCACATCTGGTGTTGTACTAAGG 109 COR-23134-4 Target 65 CACATCTGGTGTTGTACTAAGGG 110 COR-23134-4 Target 66 TTGTCTCTCTGTCTCTGACTTGG 111 COR-23134-4 Target 67 CTATTACTTCACGTTACCAGAGG 112 COR-23134-4 Target 68 TATTACTTCACGTTACCAGAGGG 113 COR-23134-4 Target 69 TCTAATATCTTGTGCAGTTTTGG 114 COR-23134-4 Target 70 ACTGCACAAGATATTAGAATAGG 115 COR-23134-4 Target 71 TTAAATAATTGACTGTATAGAGG 116 COR-23134-4 Target 72 TAGAAAGCACTCGTACATGAAGG 117 COR-23134-4 Target 73 TCTTTATTATTAGGCCGAGGGGG 118 COR-23134-4 Target 74 ATCTTTATTATTAGGCCGAGGGG 119 COR-23134-4 Target 75 TATCTTTATTATTAGGCCGAGGG 120 COR-23134-4 Target 76 TTATCTTTATTATTAGGCCGAGG 121 COR-23134-4 Target 77 AGATTCATGAGTCTTATAATAGG 122 COR-23134-4 Target 78 ATATATACTTGACAGTTGTTTGG 123 COR-23134-4 Target 79 ATACTTGACAGTTGTTTGGAAGG 124 COR-23134-4 Target 80 TCAAGTACTGCATGCAATGTAGG 125 COR-23134-4 Target 81 CTTAAATGGCAAACTCTTATTGG 126 COR-23134-4 Target 82 AATGGCAAACTCTTATTGGATGG 127 COR-23134-4 Target 83 ATTGTCCAAGTGTGACTCTGAGG 128 COR-23134-4 Target 84 TATCTCCTCAGAGTCACACTTGG 129 COR-23134-4 Target 85 ACTCACATCCAAACATAACATGG 130 COR-23134-4 Target 86 ACCAATCATACTAATTATTTTGG 131 COR-23134-4 Target 87 CAGTAGAATCTTCTTGTGAGTGG 132 COR-23134-4 Target 88 AATCTTCTTGTGAGTGGTGTGGG 133 COR-23134-4 Target 89 CTTTCTCTCGTTTCAATGCCAGG 134 COR-23134-4 Target 90 TGGTGTGGGAGTAGGCAACCTGG 135 COR-23134-4 Target 91 TGCATCCGCGGCTTAGATTGGGG 136 COR-23134-4 Target 92 TTGCATCCGCGGCTTAGATTGGG 137 COR-23134-4 Target 93 TTTGCATCCGCGGCTTAGATTGG 138 COR-23134-4 Target 94 ATCTAAGCCGCGGATGCAAACGG 139 COR-23134-4 Target 95 ACAATCCAATCTCGTTACTTAGG 140 COR-23134-4 Target 96 CAATCCAATCTCGTTACTTAGGG 141 COR-23134-4 Target 97 CACCCGGTTTCCCTATAAATTGG 142 COR-23134-4 Target 98 CTGAAGCGATACGAGTTTAGAGG 143 COR-23134-4 Target 99 ATATATGAACGAGTGTGTCTTGG 144 COR-23134-4 Target 100 CGGAATCTAGGATTTGGTAGAGG 145 COR-23134-4 Target 101 CTACCAAATCCTAGATTCCGTGG 146 COR-23134-4 Target 102 ATCCTAGATGGACCCAGTTGAGG 147 COR-23134-4 Target 103 AGTTTCACATGGATCCTAGATGG 148 COR-23134-4 Target 104 AGAAAGAGTAGAGTTTCACATGG 149 COR-23134-4 Target 105 TACTCTTTCTTTAATATCTGCGG 150 COR-23134-4 Target 106 ATTCGAGTATAGGTCACAATAGG 151 COR-23134-4 Target 107 TACAAACAAACTGGATTTGAAGG 152 COR-23134-4 Target 108 ACTGTTTAGGTACTAACTCTAGG 153 COR-23134-4 Target 109 TCGGGTGGTTCTGGAAGCGGTGG 154 COR-23134-4 Target 110 AGCGTGGTTGGGTTTGGTGAGGG 155 COR-23134-4 Target 111 TGGTGAAGGGCGCCGCCGTGGGG 156 COR-23134-4 Target 112 TTGGTGAAGGGCGCCGCCGTGGG 157 COR-23134-4 Target 113 CTCCGTGGTCGGCGCTTCCTTGG 158 COR-23134-4 Target 114 CACGGCGGCGCCCTTCACCAAGG 159 COR-23134-4 Target 115 CCGTGACACGAAGGGCTCCGTGG 160 COR-23134-4 Target 116 CACCAAGGAAGCGCCGACCACGG 161 COR-23134-4 Target 117 CCACGGAGCCCTTCGTGTCACGG 162 COR-23134-4 Target 118 CGCGCCCTTGCGAGGTTCGCCGG 163 COR-23134-4 Target 119 TTCGTGTCACGGTTCGCCTCCGG 164 COR-23134-4 Target 120 CGCCTCCGGCGAACCTCGCAAGG 165 COR-23134-4 Target 121 CGGCGAACCTCGCAAGGGCGCGG 166 COR-23134-4 Target 122 ACGACGGTGTTCGCGTACCCCGG 167 COR-23134-4 Target 123 CGGCGGAGCGCGTGAGCGCCTGG 168 COR-23134-4 Target 124 CGTGGCGCGGGAGCACGTTGCGG 169 COR-23134-4 Target 125 CGTGCTCCCGCGCCACGAGCAGG 170 COR-23134-4 Target 126 TCTGCTCACCTCCACGATCGGGG 171 COR-23134-4 Target 127 ATCTGCTCACCTCCACGATCGGG 172 COR-23134-4 Target 128 AAAGCCTCGGCGACGACGCGGGG 173 COR-23134-4 Target 129 AGAAAGCCTCGGCGACGACGCGG 174 COR-23134-4 Target 130 AGTTGCTGCTGAACGTCTTTGGG 175 COR-23134-4 Target 131 TTAACGGGCTCGTCCCAATTAGG 176 COR-23134-4 Target 132 TCCAATTGGGCCTCGGCGGGGGG 177 COR-23134-4 Target 133 TTCCAATTGGGCCTCGGCGGGGG 178 COR-23134-4 Target 134 TGTTCCAATTGGGCCTCGGCGGG 179 COR-23134-4 Target 135 ACACATTGTCAGACTCATCATGG 180 COR-23134-4 Target 136 CATTGTCAGACTCATCATGGAGG 181 COR-23134-4 Target 137 CCACCGCCGACGTAGAGAACGGG 182 COR-23134-4 Target 138 CCCGTTCTCTACGTCGGCGGTGG 183 COR-23134-4 Target 139 AAAGCGCCTCAATTCAGCACTGG 184 COR-23134-4 Target 140 TGAATTCCAGTGCTGAATTGAGG 185 COR-23134-4 Target 141 AGGCGCTTTGTTGAACTCACTGG 186 COR-23134-4 Target 142 ATGCATACCCAGCATCTGAAGGG 187 COR-23134-4 Target 143 CATGCATACCCAGCATCTGAAGG 188 COR-23134-4 Target 144 TGAATATTCCCTTCAGATGCTGG 189 COR-23134-4 Target 145 CTTCAGATGCTGGGTATGCATGG 190 COR-23134-4 Target 146 ATATTGATTCTGCCGAGATTGGG 191 COR-23134-4 Target 147 CGAGATTGGGAAGAACAAGCAGG 192 COR-23134-4 Target 148 TTCTCCGCAGCATGCTATCGAGG 193 COR-23134-4 Target 149 TGCTGCTGTTGCTAACCCTGGGG 194 COR-23134-4 Target 150 CTGTTGTGGTTGACATTGATGGG 195 COR-23134-4 Target 151 CATCATGAATGTTCAGGAGTTGG 196 COR-23134-4 Target 152 AATATCTCGCTCTCGCTAGACGG 197 COR-23134-4 Target 153 TCGGCAACACATGCTCCTGATGG 198 COR-23134-4 Target 154 CTGAGGGTGATGGTAGAACGAGG 199 COR-23134-4 Target 155 CATCAAGGAACATTTGGTCTAGG 200 COR-23134-4 Target 156 ACAGGCCAAATCCTGCAACTAGG 201 COR-23134-4 Target 157 ATCATAGTCTGTAGTAGTTTTGG 202 COR-23134-4 Target 158 TCCGTTCTACATGAACAAATTGG 203 COR-23134-4 Target 159 ACCAATTTGTTCATGTAGAACGG 204 COR-23134-4 Target 160 TTGTTCATGTAGAACGGATTTGG 205 COR-23134-4 Target 161 TAATCGACTTCAATAACAAGTGG 206 COR-23134-4 Target 162 TTGATTAAATGCTGAGGTTTAGG 207 COR-23134-4 Target 163 ACACTTCACTCTCATTAGAATGG 208 COR-23134-4 Target 164 ATGCGATGATCCTAATACTATGG 209 COR-23134-4 Target 165 TGGTATCCTACCATAGTATTAGG 210 COR-23134-4 Target 166 TATTAGGATCATCGCATTTAAGG 211 COR-23134-4 Target 167 ATAATATGGAACAAATTAGAAGG 212 COR-23134-4 Target 168 ACTTGTGCATTTGAACAAGCAGG 213 COR-23134-4 Target 169 TAATTAGTTACAATAGACTATGG 214 COR-23134-4 Target 170 TTCACCACCTTTGCCGTGGATGG 215 COR-23134-4 Target 171 ATCTTTCACCACCTTTGCCGTGG 216 COR-23134-4 Target 172 CCTGCCATCCACGGCAAAGGTGG 217 COR-23134-4 Target 173 AGAGAATGAGGAATCTCCGTTGG 218 COR-23134-4 Target 174 GTTTGAGCTCCTGCAGGATGCGG 219 COR-23134-4 Target 175 CTGCTCGTTTGAGCTCCTGCAGG 220 COR-23134-4 Target 176 GATCTGCGGCCGCATCCTGCAGG 221 COR-23134-4 Target 177 CTGCAGGAGCTCAAACGAGCAGG 222 COR-23134-4 Target 178 AACGAGCAGGAAGCAACGAGAGG 223 COR-23134-4 Target 179 ACGAGCAGGAAGCAACGAGAGGG 224 COR-23134-4 Target 180 AGCAGGAAGCAACGAGAGGGTGG 225 COR-23134-4 Target 181 CTCATGCTACGTACGCACGTCGG 226 COR-23134-4 Target 182 CACGTCCAACGTCTCCACTCAGG 227 COR-23134-4 Target 183 GTACGTAGCATGAGCCTGAGTGG 228 COR-23134-4 Target 184 ATGAGCCTGAGTGGAGACGTTGG 229 COR-23134-4 Target 185 TACATAGTTAACACGCAGAGAGG 230 COR-23134-4 Target 186 GTGTTAACTATGTACGTAAGCGG 231 COR-23134-4 Target 187 TAACTATGTACGTAAGCGGCAGG 232 COR-23134-4 Target 188 GGCAGGCAGTGCAATAAGTGTGG 233 COR-23134-4 Target 189 GGCTCTGTAGTATGTACGTGCGG 234 COR-23134-4 Target 190 GCTCTGTAGTATGTACGTGCGGG 235 COR-23134-4 Target 191 TACGATGCTGTAAGCTACTGAGG 236 COR-23134-4 Target 192 TGGATGCCAAACTCCGCAAGGGG 237 COR-23134-4 Target 193 ATGGATGCCAAACTCCGCAAGGG 238 COR-23134-4 Target 194 AATGGATGCCAAACTCCGCAAGG 239 COR-23134-4 Target 195 TCTCAGCGTAACGGCATCAATGG 240 COR-23134-4 Target 196 TGTGTCTGTTCTCAGCGTAACGG 241 COR-23134-4 Target 197 AACTATGACCCTTCATCGCTAGG 242 COR-23134-4 Target 198 TAATTTGCTGTGTTCGTACGGGG 243 COR-23134-4 Target 199 CTAATTTGCTGTGTTCGTACGGG 244 COR-23134-4 Target 200 ACTAATTTGCTGTGTTCGTACGG 245 COR-23134-4 Target 201 GTATCGTCTGAAACATGTAGGGG 246 COR-23134-4 Target 202 TGTATCGTCTGAAACATGTAGGG 247 COR-23134-4 Target 203 ATGTATCGTCTGAAACATGTAGG 248 COR-23134-4 Target 204 CAATAGCTAATTGCTAAGGATGG 249 COR-23134-4 Target 205 GGGCCAATAGCTAATTGCTAAGG 250 COR-23134-4 Target 206 CATCCTTAGCAATTAGCTATTGG 251 COR-23134-4 Target 207 ATCATTGCTTGGGATGGGCAGGG 252 COR-23134-4 Target 208 GATCATTGCTTGGGATGGGCAGG 253 COR-23134-4 Target 209 TCGAGATCATTGCTTGGGATGGG 254 COR-23134-4 Target 210 TTCGAGATCATTGCTTGGGATGG 255 COR-23134-4 Target 211 ATACTTCGAGATCATTGCTTGGG 256 COR-23134-4 Target 212 AATACTTCGAGATCATTGCTTGG 257 COR-23134-4 Target 213 AAGATTTATATCTAATCTGTTGG 258 COR-23134-4 Target 214 GTAGTTGGGATACTTAAATTTGG 259 COR-23134-4 Target 215 ACGATATGCGTTGGGTAGTTGGG 260 COR-23134-4 Target 216 AACGATATGCGTTGGGTAGTTGG 261 COR-23134-4 Target 217 AAGTTCGTGCGCCAATGAAAAGG 262 COR-23134-4 Target 218 ACATGTACGTCGGCTATAGCAGG 263 COR-23134-4 Target 219 TTGCGATGCTGAGAACGAACGGG 264 COR-23134-4 Target 220 GTTGCGATGCTGAGAACGAACGG 265 COR-23134-4 Target 221 CGCAACTCAATTTGTTATGGCGG 266 COR-23134-4 Target 222 CATTACTACCTGGGATACAAGGG 267 COR-23134-4 Target 223 GCATTACTACCTGGGATACAAGG 268 COR-23134-4 Target 224 ATATCTGTGCATTACTACCTGGG 269 COR-23134-4 Target 225 ACGCTCTTCAACTGGAAGAGCGG 270 COR-23134-4 Target 226 ACTGGAAGAGCGGTTACTACCGG 271 COR-23134-4 Target 227 GCGCTTCATGTCACCTCATAGGG 272 COR-23134-4 Target 228 AGCGCTTCATGTCACCTCATAGG 273 COR-23134-4 Target 229 ATCGAGCTAGTTACCCTATGAGG 274 COR-23134-4 Target 230 GAGGTGACATGAAGCGCTCACGG 275 COR-23134-4 Target 231 AGCGCTCACGGTTACTATGACGG 276 COR-23134-4 Target 232 ACGGTTAGCTTCACGACTGTTGG 277 COR-23134-4 Target 233 GTTAGCTTCACGACTGTTGGTGG 278 COR-23134-4 Target 234 GTAACCTAGCTAGGTAAGTCCGG 279 COR-23134-4 Target 235 GTACGACTTAGCTATAGTTCCGG 280 COR-23134-4 Target 236 CCAGCTCTGGTAACCTAGCTAGG 281 COR-23134-4 Target 237 AGTTCCGGACTTACCTAGCTAGG 282 COR-23134-4 Target 238 ATGAAGAAGGTGACCAGCTCTGG 283 COR-23134-4 Target 239 CCTAGCTAGGTTACCAGAGCTGG 284 COR-23134-4 Target 240 TGTCTTCTTAGCGATGAAGAAGG 285 COR-23134-4 Target 241 AGTTGATAGCGATATCGGTCCGG 286 COR-23134-4 Target 242 TACAAAGTTGATAGCGATATCGG 287 COR-23134-4 Target 243 AGCTAAACGCGGCCGAAGCTTGG 288 COR-23134-4 Target 244 CGGTGCATGCAAGCTAAACGCGG 289 COR-23134-4 Target 245 GTAGGCTTCTGAGGCGCGCCCGG 290 COR-23134-4 Target 246 CGCGTTTAGCTTGCATGCACCGG 291 COR-23134-4 Target 247 GCGTTTAGCTTGCATGCACCGGG 292 COR-23134-4 Target 248 TTTGCTTTGGTAGGCTTCTGAGG 293 COR-23134-4 Target 249 GATCGACAAACGCTTTGCTTTGG 294 COR-23134-4 Target 250 GCTTGCAAGCTGCAAGCAACAGG 295 COR-23134-4 Target 251 CTTGCAAGCTGCAAGCAACAGGG 296 COR-23134-4 Target 252 TTGCAAGCTGCAAGCAACAGGGG 297 COR-23134-4 Target 253 TCAATCTGGCAAGCAACAACAGG 298 COR-23134-4 Target 254 TAGATTACGTAACTCGACACAGG 299 COR-23134-4 Target 255 TGCTGATGCTATATTATCTCAGG 300 COR-23134-4 Target 256 AGATAATATAGCATCAGCACTGG 301 COR-23134-4 Target 257 TAGCATCAGCACTGGCAATCTGG 302 COR-23134-4 Target 258 AAGAGATAACAACGCATTACAGG 303 COR-23134-4 Target 259 CTAGCCGCCGACTTGGCACTTGG 304 COR-23134-4 Target 260 TCTCTTCCTAGCCGCCGACTTGG 305 COR-23134-4 Target 261 ACAGCTGCCAAGTGCCAAGTCGG 306 COR-23134-4 Target 262 GCTGCCAAGTGCCAAGTCGGCGG 307 COR-23134-4 Target 263 CAAGTGCCAAGTCGGCGGCTAGG 308 COR-23134-4 Target 264 AAGTCGGCGGCTAGGAAGAGAGG 309 COR-23134-4 Target 265 AAGTTGCTGCTGCGCCGACTTGG 310 COR-23134-4 Target 266 TGGAGCAACAGTTGCCAAGTCGG 311 COR-23134-4 Target 267 TGCTGATGGTGGCTTGGCTACGG 312 COR-23134-4 Target 268 GTATATTGCTGATGGTGGCTTGG 313 COR-23134-4 Target 269 AGCTTGTATATTGCTGATGGTGG 314 COR-23134-4 Target 270 TTTAGCTTGTATATTGCTGATGG 315 COR-23134-4 Target 271 CCTTCTGATTGCAGATTGCTTGG 316 COR-23134-4 Target 272 CCAAGCAATCTGCAATCAGAAGG 317 COR-23134-4 Target 273 CAAGCAATCTGCAATCAGAAGGG 318 COR-23134-4 Target 274 CATTTGTATTGCTACCGGTTTGG 319 COR-23134-4 Target 275 GTACACATTTGTATTGCTACCGG 320 COR-23134-4 Target 276 AAATTTGGACACAATGATTAGGG 321 COR-23134-4 Target 277 TTTGCTGTGGTAGAGCAGCATGG 322 COR-23134-4 Target 278 TATCCTTAAGTCTGGTTTGATGG 323 COR-23134-4 Target 279 GTAATGTGTATCCTTAAGTCTGG 324 COR-23134-4 Target 280 GACTTAAGGATACACATTACAGG 325 COR-23134-4 Target 281 TGGTTGTCTGGTCTGGTCCTTGG 326 COR-23134-4 Target 282 AATGAAACGGGACACGACCAAGG 327 COR-23134-4 Target 283 GGAGCTATGGTTGTCTGGTCTGG 328 COR-23134-4 Target 284 CCAATGGAGCTATGGTTGTCTGG 329 COR-23134-4 Target 285 CCAGACAACCATAGCTCCATTGG 330 COR-23134-4 Target 286 CAGACAACCATAGCTCCATTGGG 331 COR-23134-4 Target 287 CAACAGCTGCTGCTGTTCTAGGG 332 COR-23134-4 Target 288 CGACCTGGAGAGAGCCCAGAAGG 333 COR-23134-4 Target 289 AGCTGTTGTATTAGGCCTTCTGG 334 COR-23134-4 Target 290 CTTCGAGGCCGAGTCCGACCTGG 335 COR-23134-4 Target 291 AGGCCTTCTGGGCTCTCTCCAGG 336 COR-23134-4 Target 292 CTTCTGGGCTCTCTCCAGGTCGG 337 COR-23134-4 Target 293 GGCTCTCTCCAGGTCGGACTCGG 338 COR-23134-4 Target 294 CCTCGCCGACGCGACCTTCGAGG 339 COR-23134-4 Target 295 CAGGTCGGACTCGGCCTCGAAGG 340 COR-23134-4 Target 296 CTCGGCCTCGAAGGTCGCGTCGG 341 COR-23134-4 Target 297 GGAGCTGGCAGCATCAGCTCCGG 342 COR-23134-4 Target 298 CTTGTCGATGTAGAGCTCGCCGG 343 COR-23134-4 Target 299 GAGCAGCCACTCTTCGGAGCTGG 344 COR-23134-4 Target 300 ATAAGCGAGCAGCCACTCTTCGG 345 COR-23134-4 Target 301 ATGCTGCCAGCTCCGAAGAGTGG 346 COR-23134-4 Target 302 CGCGCCAACCCGGACATCATCGG 347 COR-23134-4 Target 303 GTTCAGCTTCCGCGCCAACCCGG 348 COR-23134-4 Target 304 TCGCTTATGCCGATGATGTCCGG 349 COR-23134-4 Target 305 TATGCCGATGATGTCCGGGTTGG 350 COR-23134-4 Target 306 CGATGATGTCCGGGTTGGCGCGG 351 COR-23134-4 Target 307 GGGTTGGCGCGGAAGCTGAACGG 352 COR-23134-4 Target 308 GGTTGGCGCGGAAGCTGAACGGG 353 COR-23134-4 Target 309 GCTGAACGGGTTGCTGAAGTCGG 354 COR-23134-4 Target 310 GGTTGCTGAAGTCGGTGTAGCGG 355 COR-23134-4 Target 311 GCTGAAGTCGGTGTAGCGGAAGG 356 COR-23134-4 Target 312 GGTGTAGCGGAAGGTGCGCGAGG 357 COR-23134-4 Target 313 AGCGGAAGGTGCGCGAGGTGAGG 358 COR-23134-4 Target 314 CTGCAGAAGACGATGGAGATCGG 359 COR-23134-4 Target 315 ATCTCCATCGTCTTCTGCAGCGG 360 COR-23134-4 Target 316 GCGGCATGTTGACGCTGACTTGG 361 COR-23134-4 Target 317 GCTGCCAGCACAGGAGTCGGAGG 362 COR-23134-4 Target 318 GGCGCTGCCAGCACAGGAGTCGG 363 COR-23134-4 Target 319 TTGGCCTCCGACTCCTGTGCTGG 364 COR-23134-4 Target 320 CTGTGCTGGCAGCGCCAGTGAGG 365 COR-23134-4 Target 321 GACGATGACGCGAGCGTCTCTGG 366 COR-23134-4 Target 322 AGCGCTACCGCCTCAGATTCCGG 367 COR-23134-4 Target 323 CGTCTCTGGAGCTCGCGTACCGG 368 COR-23134-4 Target 324 AGCTCGCGTACCGGAATCTGAGG 369 COR-23134-4 Target 325 TCGCGTACCGGAATCTGAGGCGG 370 COR-23134-4 Target 326 AGGCGGTAGCGCTGCGTGATCGG 371 COR-23134-4 Target 327 GGCGGTAGCGCTGCGTGATCGGG 372 COR-23134-4 Target 328 GCGGTAGCGCTGCGTGATCGGGG 373 COR-23134-4 Target 329 CGGCGACTTCGTGAGCCTCCAGG 374 COR-23134-4 Target 330 GGGAGTTGATGTTGACCTGGAGG 375 COR-23134-4 Target 331 ATCCTCAGACGCAACACGTTCGG 376 COR-23134-4 Target 332 CGCCGAACGTGTTGCGTCTGAGG 377 COR-23134-4 Target 333 ACCGGTCCCGGCTTCACCGGCGG 378 COR-23134-4 Target 334 ATCACCGGTCCCGGCTTCACCGG 379 COR-23134-4 Target 335 GCGTCTGAGGATGTCGCCGCCGG 380 COR-23134-4 Target 336 ACCTCAGTCATCACCGGTCCCGG 381 COR-23134-4 Target 337 ATGTCGCCGCCGGTGAAGCCGGG 382 COR-23134-4 Target 338 GGCGGCACCTCAGTCATCACCGG 383 COR-23134-4 Target 339 GCCGCCGGTGAAGCCGGGACCGG 384 COR-23134-4 Target 340 GCCGGGACCGGTGATGACTGAGG 385 COR-23134-4 Target 341 GTGAAGGGCTTCAGAGTCTGGGG 386 COR-23134-4 Target 342 CGTGAAGGGCTTCAGAGTCTGGG 387 COR-23134-4 Target 343 TCGTGAAGGGCTTCAGAGTCTGG 388 COR-23134-4 Target 344 AATCAGATCCCGCTCGTGAAGGG 389 COR-23134-4 Target 345 CAATCAGATCCCGCTCGTGAAGG 390 COR-23134-4 Target 346 ACTCTGAAGCCCTTCACGAGCGG 391 COR-23134-4 Target 347 CTCTGAAGCCCTTCACGAGCGGG 392 COR-23134-4 Target 348 CGGGATCTGATTGATGCGCTCGG 393 COR-23134-4 Target 349 GGGATCTGATTGATGCGCTCGGG 394 COR-23134-4 Target 350 ATTGATGCGCTCGGGATCGATGG 395 COR-23134-4 Target 351 ATCGATGGTGTTGGTGAGCGTGG 396 COR-23134-4 Target 352 TGTTGGTGAGCGTGGCTGAGCGG 397 COR-23134-4 Target 353 TGGTGAGCGTGGCTGAGCGGTGG 398 COR-23134-4 Target 354 GGTGAGCGTGGCTGAGCGGTGGG 399 COR-23134-4 Target 355 TTCGCGCACCAGTCTACAGCTGG 400 COR-23134-4 Target 356 CGGTGGGTCCAGCTGTAGACTGG 401 COR-23134-4 Target 357 AGCTGTAGACTGGTGCGCGAAGG 402 COR-23134-4 Target 358 GCTGTAGACTGGTGCGCGAAGGG 403 COR-23134-4 Target 359 AGCCACATCGGCCTCATCATCGG 404 COR-23134-4 Target 360 GAAGGGTGTTGCCGATGATGAGG 405 COR-23134-4 Target 361 AGCCACCGGCTGAGCCACATCGG 406 COR-23134-4 Target 362 TGCCGATGATGAGGCCGATGTGG 407 COR-23134-4 Target 363 ACTACGAGTCCTACAGCCACCGG 408 COR-23134-4 Target 364 TGAGGCCGATGTGGCTCAGCCGG 409 COR-23134-4 Target 365 GGCCGATGTGGCTCAGCCGGTGG 410 COR-23134-4 Target 366 GTGGCTCAGCCGGTGGCTGTAGG 411 COR-23134-4 Target 367 TGGCTGTAGGACTCGTAGTTCGG 412 COR-23134-4 Target 368 TCCCACCTGAGACCACCGAGCGG 413 COR-23134-4 Target 369 GGACTCGTAGTTCGGCCGCTCGG 414 COR-23134-4 Target 370 CTCGTAGTTCGGCCGCTCGGTGG 415 COR-23134-4 Target 371 TTCGGCCGCTCGGTGGTCTCAGG 416 COR-23134-4 Target 372 GGCCGCTCGGTGGTCTCAGGTGG 417 COR-23134-4 Target 373 GCCGCTCGGTGGTCTCAGGTGGG 418 COR-23134-4 Target 374 CCAGCTCTTCGACTCCGAGACGG 419 COR-23134-4 Target 375 CTCAGGTGGGAGCTCCGTCTCGG 420 COR-23134-4 Target 376 CCGTCTCGGAGTCGAAGAGCTGG 421 COR-23134-4 Target 377 TCTCAGCCATACCAGGGAGTCGG 422 COR-23134-4 Target 378 ACCTACTCTCAGCCATACCAGGG 423 COR-23134-4 Target 379 CACCTACTCTCAGCCATACCAGG 424 COR-23134-4 Target 380 AGAGCTGGATGCCGACTCCCTGG 425 COR-23134-4 Target 381 TGGATGCCGACTCCCTGGTATGG 426 COR-23134-4 Target 382 TCCCTGGTATGGCTGAGAGTAGG 427 COR-23134-4 Target 383 CTGGTATGGCTGAGAGTAGGTGG 428 COR-23134-4 Target 384 CCGCAGAACATCTACGAGCGCGG 429 COR-23134-4 Target 385 CCGCGCTCGTAGATGTTCTGCGG 430 COR-23134-4 Target 386 CGCGCTCGTAGATGTTCTGCGGG 431 COR-23134-4 Target 387 TCCAGTGAACGGAGTGCCCTGGG 432 COR-23134-4 Target 388 CTCCAGTGAACGGAGTGCCCTGG 433 COR-23134-4 Target 389 GATGAAGTTGAAGCGGGCCCAGG 434 COR-23134-4 Target 390 ATGAAGTTGAAGCGGGCCCAGGG 435 COR-23134-4 Target 391 CTCTTCACCACTCCAGTGAACGG 436 COR-23134-4 Target 392 GCCCAGGGCACTCCGTTCACTGG 437 COR-23134-4 Target 393 GGGCACTCCGTTCACTGGAGTGG 438 COR-23134-4 Target 394 CGTTCACTGGAGTGGTGAAGAGG 439 COR-23134-4 Target 395 TACCGGACCGAGTCCAACGCTGG 440 COR-23134-4 Target 396 GAGGATGTTCGTGCCAGCGTTGG 441 COR-23134-4 Target 397 GTTCGTGCCAGCGTTGGACTCGG 442 COR-23134-4 Target 398 TCACCTCACGCGACGTCTACCGG 443 COR-23134-4 Target 399 TGCCAGCGTTGGACTCGGTCCGG 444 COR-23134-4 Target 400 GGTCCGGTAGACGTCGCGTGAGG 445 COR-23134-4 Target 401 GTCGCGTGAGGTGAACTGCAAGG 446 COR-23134-4 Target 402 GAACAACACCTCGATCAACCCGG 447 COR-23134-4 Target 403 AGGTGAACTGCAAGGTCACCGGG 448 COR-23134-4 Target 404 CAAGGTCACCGGGTTGATCGAGG 449 COR-23134-4 Target 405 TGATCGAGGTGTTGTTCGTGAGG 450 COR-23134-4 Target 406 ACTCTCAACACGTCCACGCAGGG 451 COR-23134-4 Target 407 CACTCTCAACACGTCCACGCAGG 452 COR-23134-4 Target 408 GTTGTTCGTGAGGCCCTGCGTGG 453 COR-23134-4 Target 409 CTCAACTTCCGCCCGATCGGAGG 454 COR-23134-4 Target 410 CGCCTCAACTTCCGCCCGATCGG 455 COR-23134-4 Target 411 GTGTTGAGAGTGCCTCCGATCGG 456 COR-23134-4 Target 412 TGTTGAGAGTGCCTCCGATCGGG 457 COR-23134-4 Target 413 TGAGAGTGCCTCCGATCGGGCGG 458 COR-23134-4 Target 414 CTCCGATCGGGCGGAAGTTGAGG 459 COR-23134-4 Target 415 CGATCGGGCGGAAGTTGAGGCGG 460 COR-23134-4 Target 416 CAGCACATGAACTACTGGGTCGG 461 COR-23134-4 Target 417 CACGCAGCACATGAACTACTGGG 462 COR-23134-4 Target 418 CCACGCAGCACATGAACTACTGG 463 COR-23134-4 Target 419 CCAGTAGTTCATGTGCTGCGTGG 464 COR-23134-4 Target 420 TCTACTCCGCATCCTCACGCTGG 465 COR-23134-4 Target 421 CTGCGTGGAGCTCCAGCGTGAGG 466 COR-23134-4 Target 422 GGAGCTCCAGCGTGAGGATGCGG 467 COR-23134-4 Target 423 GCGTGAGGATGCGGAGTAGATGG 468 COR-23134-4 Target 424 GCCACACCTCCTGGACTTCCCGG 469 COR-23134-4 Target 425 GAGTAGATGGTCAGCTGCTCCGG 470 COR-23134-4 Target 426 AGTAGATGGTCAGCTGCTCCGGG 471 COR-23134-4 Target 427 CTTCCGCCCGCCACACCTCCTGG 472 COR-23134-4 Target 428 TCAGCTGCTCCGGGAAGTCCAGG 473 COR-23134-4 Target 429 GCTGCTCCGGGAAGTCCAGGAGG 474 COR-23134-4 Target 430 AGTCCAGGAGGTGTGGCGGGCGG 475 COR-23134-4 Target 431 GAGGTGTGGCGGGCGGAAGATGG 476 COR-23134-4 Target 432 GCCATCCTTCAGCGCCATAGAGG 477 COR-23134-4 Target 433 GCGGAAGATGGCTGCCTCTATGG 478 COR-23134-4 Target 434 GGCTGCCTCTATGGCGCTGAAGG 479 COR-23134-4 Target 435 GCCTCTATGGCGCTGAAGGATGG 480 COR-23134-4 Target 436 CAGGATTCGCCAGCACCAACTGG 481 COR-23134-4 Target 437 CGCGTTGTTGTTGAACCAGTTGG 482 COR-23134-4 Target 438 GTTGTTGAACCAGTTGGTGCTGG 483 COR-23134-4 Target 439 GGTCGCACCAACGCTCCGTCAGG 484 COR-23134-4 Target 440 TTGGTGCTGGCGAATCCTGACGG 485 COR-23134-4 Target 441 GGCGAATCCTGACGGAGCGTTGG 486 COR-23134-4 Target 442 GAGATCTACACCGATCCCATCGG 487 COR-23134-4 Target 443 CGGAGCGTTGGTGCGACCGATGG 488 COR-23134-4 Target 444 GGAGCGTTGGTGCGACCGATGGG 489 COR-23134-4 Target 445 GTTGGTGCGACCGATGGGATCGG 490 COR-23134-4 Target 446 TGTAGATCTCGCGCGTCAGCTGG 491 COR-23134-4 Target 447 GATCTCGCGCGTCAGCTGGGCGG 492 COR-23134-4 Target 448 AGCTGGGCGGACGTGTTGATCGG 493 COR-23134-4 Target 449 GCTGGGCGGACGTGTTGATCGGG 494 COR-23134-4 Target 450 ACGTGTTGATCGGGTACGTGCGG 495 COR-23134-4 Target 451 CGTGTTGATCGGGTACGTGCGGG 496 COR-23134-4 Target 452 TACGTGCGGGTGTCGTAGCTTGG 497 COR-23134-4 Target 453 ACGTGCGGGTGTCGTAGCTTGGG 498 COR-23134-4 Target 454 AGCTTGGGAAGAGTGCGACGAGG 499 COR-23134-4 Target 455 TTCCGCAGGGACCTCACGCTCGG 500 COR-23134-4 Target 456 GGTCGAGGACGCCGAGCGTGAGG 501 COR-23134-4 Target 457 CCGGTACAACCAGTTCCGCAGGG 502 COR-23134-4 Target 458 TCCGGTACAACCAGTTCCGCAGG 503 COR-23134-4 Target 459 CGCCGAGCGTGAGGTCCCTGCGG 504 COR-23134-4 Target 460 GCGTGAGGTCCCTGCGGAACTGG 505 COR-23134-4 Target 461 CCAACGCGGAGTCCTGGCTCCGG 506 COR-23134-4 Target 462 CCCTGCGGAACTGGTTGTACCGG 507 COR-23134-4 Target 463 GCGGCACCAACGCGGAGTCCTGG 508 COR-23134-4 Target 464 GAACTGGTTGTACCGGAGCCAGG 509 COR-23134-4 Target 465 CAACCTCCGCGGCACCAACGCGG 510 COR-23134-4 Target 466 CCGGAGCCAGGACTCCGCGTTGG 511 COR-23134-4 Target 467 ACCGGCCTGAACAACCTCCGCGG 512 COR-23134-4 Target 468 AGGACTCCGCGTTGGTGCCGCGG 513 COR-23134-4 Target 469 ACTCCGCGTTGGTGCCGCGGAGG 514 COR-23134-4 Target 470 TGGTGCCGCGGAGGTTGTTCAGG 515 COR-23134-4 Target 471 GCCGCGGAGGTTGTTCAGGCCGG 516 COR-23134-4 Target 472 ACTCCAACCACTGCGTCCAGTGG 517 COR-23134-4 Target 473 TCAGGCCGGTGTTGTACCACTGG 518 COR-23134-4 Target 474 TGTTGTACCACTGGACGCAGTGG 519 COR-23134-4 Target 475 GTACCACTGGACGCAGTGGTTGG 520 COR-23134-4 Target 476 GGAGCAGATCCGCTACACCGAGG 521 COR-23134-4 Target 477 GCAGTGGTTGGAGTACTCCTCGG 522 COR-23134-4 Target 478 TGGAGTACTCCTCGGTGTAGCGG 523 COR-23134-4 Target 479 CGACGTCAACCAGTACTACCAGG 524 COR-23134-4 Target 480 CGGTGTAGCGGATCTGCTCCTGG 525 COR-23134-4 Target 481 GGATCTGCTCCTGGTAGTACTGG 526 COR-23134-4 Target 482 CTGGTAGTACTGGTTGACGTCGG 527 COR-23134-4 Target 483 AGCCTCTTCGGCAGCGAGTGGGG 528 COR-23134-4 Target 484 TAGCCTCTTCGGCAGCGAGTGGG 529 COR-23134-4 Target 485 CTAGCCTCTTCGGCAGCGAGTGG 530 COR-23134-4 Target 486 CTCCGCGACGCTAGCCTCTTCGG 531 COR-23134-4 Target 487 TGCCGAAGAGGCTAGCGTCGCGG 532 COR-23134-4 Target 488 TAGCGTCGCGGAGCAGCAGCAGG 533 COR-23134-4 Target 489 CGTCGCGGAGCAGCAGCAGGTGG 534 COR-23134-4 Target 490 ACTCCTCATGGTCTACGCACAGG 535 COR-23134-4 Target 491 CCAGGAGGTGCCACTCCTCATGG 536 COR-23134-4 Target 492 CAGCCTGTGCGTAGACCATGAGG 537 COR-23134-4 Target 493 TGTGCGTAGACCATGAGGAGTGG 538 COR-23134-4 Target 494 CTTCAGCATCCGGAACCAGGAGG 539 COR-23134-4 Target 495 GCTCTTCAGCATCCGGAACCAGG 540 COR-23134-4 Target 496 CCATGAGGAGTGGCACCTCCTGG 541 COR-23134-4 Target 497 CCATCCCGCTCTTCAGCATCCGG 542 COR-23134-4 Target 498 GGAGTGGCACCTCCTGGTTCCGG 543 COR-23134-4 Target 499 TGGTTCCGGATGCTGAAGAGCGG 544 COR-23134-4 Target 500 GGTTCCGGATGCTGAAGAGCGGG 545 COR-23134-4 Target 501 GATGCTGAAGAGCGGGATGGCGG 546 COR-23134-4 Target 502 CGAGTGCGACGTAGCGCTCGAGG 547 COR-23134-4 Target 503 GACGTAGCGCTCGAGGATGATGG 548 COR-23134-4 Target 504 ACCGGAACGACGCTCGCAGCAGG 549 COR-23134-4 Target 505 TCGAGACCTGGCTGGACAACCGG 550 COR-23134-4 Target 506 ACCTGCTGCGAGCGTCGTTCCGG 551 COR-23134-4 Target 507 GCAGGCTCTCGAGACCTGGCTGG 552 COR-23134-4 Target 508 ACCAGCAGGCTCTCGAGACCTGG 553 COR-23134-4 Target 509 GTCGTTCCGGTTGTCCAGCCAGG 554 COR-23134-4 Target 510 CGGATACCGGTCCTACCAGCAGG 555 COR-23134-4 Target 511 GCCAGGTCTCGAGAGCCTGCTGG 556 COR-23134-4 Target 512 GGTCTCGAGAGCCTGCTGGTAGG 557 COR-23134-4 Target 513 AGGGACTCGGACGCGGATACCGG 558 COR-23134-4 Target 514 CGAGAGCCTGCTGGTAGGACCGG 559 COR-23134-4 Target 515 CGCTTGGAGGGACTCGGACGCGG 560 COR-23134-4 Target 516 ATCGCACGCTTGGAGGGACTCGG 561 COR-23134-4 Target 517 ACCGCGATCGCACGCTTGGAGGG 562 COR-23134-4 Target 518 CACCGCGATCGCACGCTTGGAGG 563 COR-23134-4 Target 519 GAACACCGCGATCGCACGCTTGG 564 COR-23134-4 Target 520 TCCCTCCAAGCGTGCGATCGCGG 565 COR-23134-4 Target 521 AGCAGGTGACCGAGAACACCCGG 566 COR-23134-4 Target 522 AGCGTGCGATCGCGGTGTTCCGG 567 COR-23134-4 Target 523 GCGTGCGATCGCGGTGTTCCGGG 568 COR-23134-4 Target 524 CGCGGTGTTCCGGGTGTTCTCGG 569 COR-23134-4 Target 525 CGAGCAACTCATCCGGCAGCAGG 570 COR-23134-4 Target 526 AGCACGTCGAGCAACTCATCCGG 571 COR-23134-4 Target 527 TGTTCTCGGTCACCTGCTGCCGG 572 COR-23134-4 Target 528 TGAGTTGCTCGACGTGCTCCAGG 573 COR-23134-4 Target 529 GTGCTCCAGGAAGATCTCCCAGG 574 COR-23134-4 Target 530 TGCTCCAGGAAGATCTCCCAGGG 575 COR-23134-4 Target 531 GCTCCAGGAAGATCTCCCAGGGG 576 COR-23134-4 Target 532 GTCGGAGAGCTCTGGCCATCAGG 577 COR-23134-4 Target 533 AGCTTCTACAGCTTCCTCGTCGG 578 COR-23134-4 Target 534 ATGGCCAGAGCTCTCCGACGAGG 579 COR-23134-4 Target 535 GACGAGGAAGCTGTAGAAGCTGG 580 COR-23134-4 Target 536 GTCCTCGGAGTGCCATTCGCCGG 581 COR-23134-4 Target 537 GTAGAAGCTGGCGAGCTGTCCGG 582 COR-23134-4 Target 538 CTGGCGAGCTGTCCGGCGAATGG 583 COR-23134-4 Target 539 GGCCGCATACTCGGAGTCCTCGG 584 COR-23134-4 Target 540 GTCCGGCGAATGGCACTCCGAGG 585 COR-23134-4 Target 541 TCCATCGCAGGCCGCATACTCGG 586 COR-23134-4 Target 542 CTCCGAGGACTCCGAGTATGCGG 587 COR-23134-4 Target 543 CAGACTGGCATCTCCATCGCAGG 588 COR-23134-4 Target 544 TCCGAGTATGCGGCCTGCGATGG 589 COR-23134-4 Target 545 AGCGCGTCCACCGTGCAGACTGG 590 COR-23134-4 Target 546 GATGGAGATGCCAGTCTGCACGG 591 COR-23134-4 Target 547 GGAGATGCCAGTCTGCACGGTGG 592 COR-23134-4 Target 548 ACGGTGGACGCGCTGACGAACGG 593 COR-23134-4 Target 549 CGGTGGACGCGCTGACGAACGGG 594 COR-23134-4 Target 550 AGACAGCCTCTGCGTCGCCGAGG 595 COR-23134-4 Target 551 CGGGTCGATGTTGTTGACCTCGG 596 COR-23134-4 Target 552 TGTTGACCTCGGCGACGCAGAGG 597 COR-23134-4 Target 553 TGGACTTGTCGCTGGACGCCCGG 598 COR-23134-4 Target 554 CGCAGAGGCTGTCTTCGATCCGG 599 COR-23134-4 Target 555 GCAGAGGCTGTCTTCGATCCGGG 600 COR-23134-4 Target 556 GAGCAACCACAGCGCTCAGATGG 601 COR-23134-4 Target 557 ACAAGTCCATCTGAGCGCTGTGG 602 COR-23134-4 Target 558 TAATCTCGTTCTCGTTCTTCCGG 603 COR-23134-4 Target 559 TTCTCGTTCTTCCGGTTGCTCGG 604 COR-23134-4 Target 560 TCTCGTTCTTCCGGTTGCTCGGG 605 COR-23134-4 Target 561 CTCGTTCTTCCGGTTGCTCGGGG 606 COR-23134-4 Target 562 ACTTAGCCTAGGATCCGCCATGG 607 COR-23134-4 Target 563 GGTCGACTTTAACTTAGCCTAGG 608 COR-23134-4 Target 564 TTGTTTGGTGTTACTTCTGCAGG 609 COR-23134-4 Target 565 TCGATGCTCACCCTGTTGTTTGG 610 COR-23134-4 Target 566 AGAAGTAACACCAAACAACAGGG 611 COR-23134-4 Target 567 TCATACGCTATTTATTTGCTTGG 612 COR-23134-4 Target 568 AATAAATAGCGTATGAAGGCAGG 613 COR-23134-4 Target 569 ATAAATAGCGTATGAAGGCAGGG 614 COR-23134-4 Target 570 ATCTTGATATACTTGGATGATGG 615 COR-23134-4 Target 571 GATAGGTATACATGTTGATGTGG 616 COR-23134-4 Target 572 TGGAAATATCGATCTAGGATAGG 617 COR-23134-4 Target 573 ATGGATGGAAATATCGATCTAGG 618 COR-23134-4 Target 574 AGTTACGAGTTTAAGATGGATGG 619 COR-23134-4 Target 575 TCATAGTTACGAGTTTAAGATGG 620 COR-23134-4 Target 576 CCTGGTGTATTTATTAATTTTGG 621 COR-23134-4 Target 577 GAATACTGTTTCAAACTACCTGG 622 COR-23134-4 Target 578 GTCGTTCATTCGTTCTAGATCGG 623 COR-23134-4 Target 579 GATGATGTGGTGTGGTTGGGCGG 624 COR-23134-4 Target 580 TTGTGATGATGTGGTGTGGTTGG 625 COR-23134-4 Target 581 ATAGGTATACATGTTGATGCGGG 626 COR-23134-4 Target 582 GATAGGTATACATGTTGATGCGG 627 COR-23134-4 Target 583 TGGAAATATCGATCTAGGATAGG 628 COR-23134-4 Target 584 ATGGATGGAAATATCGATCTAGG 629 COR-23134-4 Target 585 TACGAATTGAAGATGATGGATGG 630 COR-23134-4 Target 586 TAGTTACGAATTGAAGATGATGG 631 COR-23134-4 Target 587 TTCGTAACTATGAATATGTATGG 632 COR-23134-4 Target 588 TTCTGTTTCAAACTACCTGGTGG 633 COR-23134-4 Target 589 GAATTCTGTTTCAAACTACCTGG 634 COR-23134-4 Target 590 GTTGGGCGGTCGTTCTAGATCGG 635 COR-23134-4 Target 591 GATGATGTGGTGTGGTTGGGCGG 636 COR-23134-4 Target 592 TTGTGATGATGTGGTGTGGTTGG 637 COR-23134-4 Target 593 ATGCCGTGCACTTGTTTGTCGGG 638 COR-23134-4 Target 594 TGACCCGACAAACAAGTGCACGG 639 COR-23134-4 Target 595 CACGGCATATATTGAAATAAAGG 640 COR-23134-4 Target 596 TTGTTTCGTTGCATAGGGTTTGG 641 COR-23134-4 Target 597 GCTCTAGCCGTTCCGCAGACGGG 642 COR-23134-4 Target 598 GGCTCTAGCCGTTCCGCAGACGG 643 COR-23134-4 Target 599 CATGAAATCGATCCCGTCTGCGG 644 COR-23134-4 Target 600 AATCGATCCCGTCTGCGGAACGG 645 COR-23134-4 Target 601 GGAACGGCTAGAGCCATCCCAGG 646 COR-23134-4 Target 602 CTTGCCAGTGTTTCTCTTTGGGG 647 COR-23134-4 Target 603 ACTTGCCAGTGTTTCTCTTTGGG 648 COR-23134-4 Target 604 TCAGAACGTGTCTGACGTACAGG 649 COR-23134-4 Target 605 GTGCTGCTAGCGTTCGTACACGG 650 COR-23134-4 Target 606 GTGTACGAACGCTAGCAGCACGG 651 COR-23134-4 Target 607 GCACGGATCTAACACAAACACGG 652 COR-23134-4 Target 608 GTCCATGCATGGTTAGGGCCCGG 653 COR-23134-4 Target 609 TGAACAGAAGTAGAACTACCGGG 654 COR-23134-4 Target 610 TTCCGGTCCATGCATGGTTAGGG 655 COR-23134-4 Target 611 GTTCCGGTCCATGCATGGTTAGG 656 COR-23134-4 Target 612 TCGGCGTTCCGGTCCATGCATGG 657 COR-23134-4 Target 613 TACCGGGCCCTAACCATGCATGG 658 COR-23134-4 Target 614 CCTTCTCTAGATCGGCGTTCCGG 659 COR-23134-4 Target 615 GGCCCTAACCATGCATGGACCGG 660 COR-23134-4 Target 616 CCTCTCTACCTTCTCTAGATCGG 661 COR-23134-4 Target 617 CCGGAACGCCGATCTAGAGAAGG 662 COR-23134-4 Target 618 CCGATCTAGAGAAGGTAGAGAGG 663 COR-23134-4 Target 619 TCTAGAGAAGGTAGAGAGGGGGG 664 COR-23134-4 Target 620 CCGTCGGCACCTCCGCTTCAAGG 665 COR-23134-4 Target 621 ACGAGCGGCGTACCTTGAAGCGG 666 COR-23134-4 Target 622 AGCGGCGTACCTTGAAGCGGAGG 667 COR-23134-4 Target 623 CTTGAAGCGGAGGTGCCGACGGG 668 COR-23134-4 Target 624 GAAGCGGAGGTGCCGACGGGTGG 669 COR-23134-4 Target 625 GAGGTGCCGACGGGTGGATTTGG 670 COR-23134-4 Target 626 AGGTGCCGACGGGTGGATTTGGG 671 COR-23134-4 Target 627 GGTGCCGACGGGTGGATTTGGGG 672 COR-23134-4 Target 628 GTGCCGACGGGTGGATTTGGGGG 673 COR-23134-4 Target 629 TGTGCGCTCCGAACAACACGAGG 674 COR-23134-4 Target 630 CGCTCCGAACAACACGAGGTTGG 675 COR-23134-4 Target 631 GCTCCGAACAACACGAGGTTGGG 676 COR-23134-4 Target 632 CTCCGAACAACACGAGGTTGGGG 677 COR-23134-4 Target 633 CATCTGCAGGTCGACTCTAGGGG 678 COR-23134-4 Target 634 CGCATCTGCAGGTCGACTCTAGG 679 COR-23134-4 Target 635 CTAGAGTCGACCTGCAGATGCGG 680 COR-23134-4 Target 636 CGACCTGCAGATGCGGCTGCTGG 681 COR-23134-4 Target 637 GACCTGCAGATGCGGCTGCTGGG 682 COR-23134-4 Target 638 AAGGCTGCGAATGCGATGCGAGG 683 COR-23134-4 Target 639 AGGCTGCGAATGCGATGCGAGGG 684 COR-23134-4 Target 640 TGCGATGCGAGGGATTCGCTCGG 685 COR-23134-4 Target 641 ATTCGCTCGGTCGATTTCTATGG 686 COR-23134-4 Target 642 CGCACCCATTGCTCTTCTCACGG 687 COR-23134-4 Target 643 TATGGCCGTGAGAAGAGCAATGG 688 COR-23134-4 Target 644 ATGGCCGTGAGAAGAGCAATGGG 689 COR-23134-4 Target 645 AATGGGTGCGGCGAATTTAAAGG 690 COR-23134-4 Target 646 GTGCGGCGAATTTAAAGGAAAGG 691 COR-23134-4 Target 647 GCGGCGAATTTAAAGGAAAGGGG 692 COR-23134-4 Target 648 GGGCAACTTGGGCTGATTGGTGG 693 COR-23134-4 Target 649 AACTTGGGCTGATTGGTGGAAGG 694 COR-23134-4 Target 650 CTATCCACGTCAGCGATCCGTGG 695 COR-23134-4 Target 651 TGGTGGAAGGGCTAGTGCCACGG 696 COR-23134-4 Target 652 AGTGCCACGGATCGCTGACGTGG 697 COR-23134-4 Target 653 ATCGCTGACGTGGATAGAAGTGG 698 COR-23134-4 Target 654 TGTGTTCAATCTGTCGCAAGTGG 699 COR-23134-4 Target 655 TTCAATCTGTCGCAAGTGGACGG 700 COR-23134-4 Target 656 TCTGACCACGTCGACAATCCGGG 701 COR-23134-4 Target 657 CTCTGACCACGTCGACAATCCGG 702 COR-23134-4 Target 658 GGTGCAGATGTGTTTCGGCCCGG 703 COR-23134-4 Target 659 TTCGGCCCGGATTGTCGACGTGG 704 COR-23134-4 Target 660 GACGTGGTCAGAGTCTGAAGCGG 705 COR-23134-4 Target 661 ACGTGGTCAGAGTCTGAAGCGGG 706 COR-23134-4 Target 662 TGGTCAGAGTCTGAAGCGGGAGG 707 COR-23134-4 Target 663 CTGAAGCGGGAGGAGACTGAAGG 708 COR-23134-4 Target 664 CGCCCACCTTTGCGCGCCGAAGG 709 COR-23134-4 Target 665 AGACTGAAGGAACTGACCTTCGG 710 COR-23134-4 Target 666 AACTGACCTTCGGCGCGCAAAGG 711 COR-23134-4 Target 667 TGACCTTCGGCGCGCAAAGGTGG 712 COR-23134-4 Target 668 GACCTTCGGCGCGCAAAGGTGGG 713 COR-23134-4 Target 669 CTTCGGCGCGCAAAGGTGGGCGG 714 COR-23134-4 Target 670 TTCGGCGCGCAAAGGTGGGCGGG 715 COR-23134-4 Target 671 CCGCCAAAGGAAAGGCCTTCCGG 716 COR-23134-4 Target 672 GCGCGCAAAGGTGGGCGGGCCGG 717 COR-23134-4 Target 673 GCAAAGGTGGGCGGGCCGGAAGG 718 COR-23134-4 Target 674 GGGCCGGAAGGCCTTTCCTTTGG 719 COR-23134-4 Target 675 AAGGCCTTTCCTTTGGCGGTTGG 720 COR-23134-4 Target 676 ATCCTGTAGAAAGTTGGGCCAGG 721 COR-23134-4 Target 677 AAAGTTATCCTGTAGAAAGTTGG 722 COR-23134-4 Target 678 GGCCTGGCCCAACTTTCTACAGG 723 COR-23134-4 Target 679 TAGACAGTTGGCGGCCTGGCGGG 724 COR-23134-4 Target 680 CTAGACAGTTGGCGGCCTGGCGG 725 COR-23134-4 Target 681 ACACTAGACAGTTGGCGGCCTGG 726 COR-23134-4 Target 682 ACAGGATAACTTTACCCGCCAGG 727 COR-23134-4 Target 683 TATTTACACTAGACAGTTGGCGG 728 COR-23134-4 Target 684 GTGTATTTACACTAGACAGTTGG 729 COR-23134-4 Target 685 TCTACTACAGTAAGTATTATTGG 730 COR-23134-4 Target 686 CATTTCTGGCACGTGACAACTGG 731 COR-23134-4 Target 687 CTATTCAAATGTGACATTTCTGG 732 COR-23134-4 Target 688 ATGTCACATTTGAATAGAGGAGG 733 COR-23134-4 Target 689 ACACCAATTTAAGGCATTGTTGG 734 COR-23134-4 Target 690 AATTGAAATATGGTGCTCTACGG 735 COR-23134-4 Target 691 TTTATGAAATGAGACACTATTGG 736 COR-23134-4 Target 692 TATATTTGAGGCACTTTAGAGGG 737 COR-23134-4 Target 693 GTATATTTGAGGCACTTTAGAGG 738 COR-23134-4 Target 694 ACTATGATATAGTATATTTGAGG 739 COR-23134-4 Target 695 AAAGCCTTAAATCTATTATAGGG 740 COR-23134-4 Target 696 TAAAGCCTTAAATCTATTATAGG 741 COR-23134-4 Target 697 GCTTCCCTATAATAGATTTAAGG 742 COR-23134-4 Target 698 TAGATTTAAGGCTTTAATGTTGG 743 COR-23134-4 Target 699 TTAAGGCTTTAATGTTGGAGCGG 744 COR-23134-4 Target 700 GCGATACATCATATGGACAGGGG 745 COR-23134-4 Target 701 TGCGATACATCATATGGACAGGG 746 COR-23134-4 Target 702 GTGCGATACATCATATGGACAGG 747 COR-23134-4 Target 703 CTTGAGTGCGATACATCATATGG 748 COR-23134-4 Target 704 ATGTCAAGTCTTACAAGTCCAGG 749 COR-23134-4 Target 705 TGATGTAGTAACTAATAAGAAGG 750 COR-23134-4 Target 706 TGCAGAGTTATACAGTCACTAGG 751 COR-23134-4 Target 707 TTACGCATATCGCAACACATGGG 752 COR-23134-4 Target 708 TTTACGCATATCGCAACACATGG 753 COR-23134-4 Target 709 ATCTATGTGGAAACATTTATCGG 754 COR-23134-4 Target 710 CGGCCGCAGGTCGACTCTAGAGG 755 COR-23134-4 Target 711 TGAAGCCCAAACGCGGCCGCAGG 756 COR-23134-4 Target 712 GATCCTCTAGAGTCGACCTGCGG 757 COR-23134-4 Target 713 ACAAACTTGAAGCCCAAACGCGG 758 COR-23134-4 Target 714 AGTCGACCTGCGGCCGCGTTTGG 759 COR-23134-4 Target 715 GTCGACCTGCGGCCGCGTTTGGG 760 COR-23134-4 Target 716 GTCACTTACCGGATTCTGGCCGG 761 COR-23134-4 Target 717 CCTAGTCACTTACCGGATTCTGG 762 COR-23134-4 Target 718 AGCAGGCTCCGGCCAGAATCCGG 763 COR-23134-4 Target 719 CCAGAATCCGGTAAGTGACTAGG 764 COR-23134-4 Target 720 CAGAATCCGGTAAGTGACTAGGG 765 COR-23134-4 Target 721 TGGCCGAATTTAAGTGACTAGGG 766 COR-23134-4 Target 722 CTGGCCGAATTTAAGTGACTAGG 767 COR-23134-4 Target 723 TGACCCTAGTCACTTAAATTCGG 768 COR-23134-4 Target 724 GCGCGCGCGCCGTCCCATTCTGG 769 COR-23134-4 Target 725 TCACTTAAATTCGGCCAGAATGG 770 COR-23134-4 Target 726 CACTTAAATTCGGCCAGAATGGG 771 COR-23134-4 Target 727 TAAATTCGGCCAGAATGGGACGG 772 COR-23134-4 Target 728 TGGTTCGGTTTAAACAGCCCGGG 773 COR-23134-4 Target 729 CTGGTTCGGTTTAAACAGCCCGG 774 COR-23134-4 Target 730 AATGGGACGGCGCGCGCGCCCGG 775 COR-23134-4 Target 731 ATGGGACGGCGCGCGCGCCCGGG 776 COR-23134-4 Target 732 TCGGCCTAACTAAACTGGTTCGG 777 COR-23134-4 Target 733 ATAAGTCGGCCTAACTAAACTGG 778 COR-23134-4 Target 734 CCTAGACTAGTTCAATAAGTCGG 779 COR-23134-4 Target 735 TTGAACTAGTCTAGGGCGTTAGG 780 COR-23134-4 Target 736 AGGGCGTTAGGAACTCTCTAAGG 781 COR-23134-4 Target 737 GGGCGTTAGGAACTCTCTAAGGG 782 COR-23134-4 Target 738 GGCGTTAGGAACTCTCTAAGGGG 783 COR-23134-4 Target 739 CTCCAGTGTTTCTGTGGCCGCGG 784 COR-23134-4 Target 740 GCGTGTTAGAGGCTTCGCCGCGG 785 COR-23134-4 Target 741 TGGATTCTCCAGTGTTTCTGTGG 786 COR-23134-4 Target 742 CGCCGCGGCCACAGAAACACTGG 787 COR-23134-4 Target 743 CCAGGTTATTATTATGATGGTGG 788 COR-23134-4 Target 744 GATCCAGGTTATTATTATGATGG 789 COR-23134-4 Target 745 GTTATTTATTTAAGCGATCCAGG 790 COR-23134-4 Target 746 GATCGCTTAAATAAATAACATGG 791 COR-23134-4 Target 747 AAACTAAGCTCGACAGAACCAGG 792 COR-23134-4 Target 748 AATGGAGCCTAGCAAGAACATGG 793 COR-23134-4 Target 749 GGAGCCTAGCAAGAACATGGAGG 794 COR-23134-4 Target 750 TTCGTCTCCTAATATTTGCCTGG 795 COR-23134-4 Target 751 TTTGATCCCAGGCAAATATTAGG 796 COR-23134-4 Target 752 CTCTGCTCAATATGGTATTATGG 797 COR-23134-4 Target 753 TGATTTGCCCATAACAGTTTTGG 798 COR-23134-4 Target 754 ACACAGGACACGAACAGTATTGG 799 COR-23134-4 Target 755 CACAGGACACGAACAGTATTGGG 800 COR-23134-4 Target 756 TGTCGTCTATCAAGTATCTGTGG 801 COR-23134-4 Target 757 TGCCTTCTGGTGTACCTGGGAGG 802 COR-23134-4 Target 758 TGGTGCCTTCTGGTGTACCTGGG 803 COR-23134-4 Target 759 CTGGTGCCTTCTGGTGTACCTGG 804 COR-23134-4 Target 760 TTGATAGACGACAACCTCCCAGG 805 COR-23134-4 Target 761 CTGTGTCGTCTGGTGCCTTCTGG 806 COR-23134-4 Target 762 AACCTCCCAGGTACACCAGAAGG 807 COR-23134-4 Target 763 GCCGCTGTACCTGTGTCGTCTGG 808 COR-23134-4 Target 764 CAGAAGGCACCAGACGACACAGG 809 COR-23134-4 Target 765 ACCAGACGACACAGGTACAGCGG 810 COR-23134-4 Target 766 CACTTATGGGATATCGCCGTGGG 811 COR-23134-4 Target 767 GCACTTATGGGATATCGCCGTGG 812 COR-23134-4 Target 768 GCGGCAGCTTAAACGACCCACGG 813 COR-23134-4 Target 769 AGACCTAATCAAGCACTTATGGG 814 COR-23134-4 Target 770 AAGACCTAATCAAGCACTTATGG 815 COR-23134-4 Target 771 CAAAGTCGAATCGCTCATTAAGG 816 COR-23134-4 Target 772 ATACAAGCGTCTCTATGTCGCGG 817 COR-23134-4 Target 773 TACAAGCGTCTCTATGTCGCGGG 818 COR-23134-4 Target 774 AGAAACTCACGCGAGAATGTTGG 819 COR-23134-4 Target 775 AGAATGTTGGAATTGCTCTAAGG 820 COR-23134-4 Target 776 GAATGTTGGAATTGCTCTAAGGG 821 COR-23134-4 Target 777 AGTTTATCCTAGAGAATCATGGG 822 COR-23134-4 Target 778 GAGTTTATCCTAGAGAATCATGG 823 COR-23134-4 Target 779 TTTGATTCCCATGATTCTCTAGG 824 COR-23134-4 Target 780 TTGATTTAGCCTGCAAGATATGG 825 COR-23134-4 Target 781 TGATTTAGCCTGCAAGATATGGG 826 COR-23134-4 Target 782 GATTTAGCCTGCAAGATATGGGG 827 COR-23134-4 Target 783 TTTCCTAGAGCTCTACCTCTAGG 828 COR-23134-4 Target 784 GTTCCTAGAGGTAGAGCTCTAGG 829 COR-23134-4 Target 785 TGGTAAACTGAAGTTCAGAATGG 830 COR-23134-4 Target 786 AAAGCAAACTCTGAGGCAACAGG 831 COR-23134-4 Target 787 TATTCGTCGCCGCGCCGCACTGG 832 COR-23134-4 Target 788 CAGGTTACACCAGTGCGGCGCGG 833 COR-23134-4 Target 789 TGTGCTCCTGCTGTATGAAATGG 834 COR-23134-4 Target 790 TGCGATCCATTTCATACAGCAGG 835 COR-23134-4 Target 791 GACAAATATGACTCATCTTCAGG 836 COR-23134-4 Target 792 TATGACTCATCTTCAGGAATCGG 837 COR-23134-4 Target 793 ACATGATACTGCCATGATAAGGG 838 COR-23134-4 Target 794 TACATGATACTGCCATGATAAGG 839 COR-23134-4 Target 795 ATCATGGCAGTATCATGTATTGG 840 COR-23134-4 Target 796 AGTATCATGTATTGGTTCACAGG 841 COR-23134-4 Target 797 GATGGTTCGTAAGGTGTTACTGG 842 COR-23134-4 Target 798 GAAGTTCGAGATGGTTCGTAAGG 843 COR-23134-4 Target 799 GTAGACGCTGCTAGTGACTAAGG 844 COR-23134-4 Target 800 GCTGCTAGTGACTAAGGTTTAGG 845 COR-23134-4 Target 801 CGAAGACTAACGGTTAGCTAGGG 846 COR-23134-4 Target 802 GCGAAGACTAACGGTTAGCTAGG 847 COR-23134-4 Target 803 CACTTAGGAGCGAAGACTAACGG 848 COR-23134-4 Target 804 CCTGAATTCTGCAGGCACTTAGG 849 COR-23134-4 Target 805 GGTACCGACCTGAATTCTGCAGG 850 COR-23134-4 Target 806 CCTAAGTGCCTGCAGAATTCAGG 851 COR-23134-4 Target 807 AGTGCCTGCAGAATTCAGGTCGG 852 COR-23134-4 Target 808 TTCGATCGCGTCGCCGTCACCGG 853 COR-23134-4 Target 809 TGCAGAATTCAGGTCGGTACCGG 854 COR-23134-4 Target 810 ATTCAGGTCGGTACCGGTGACGG 855 COR-23134-4 Target 811 TGACGGCGACGCGATCGAACAGG 856 COR-23134-4 Target 812 CGGCGACGCGATCGAACAGGTGG 857 COR-23134-4 Target 813 GAGCCATCTCTTGACCCAGCCGG 858 COR-23134-4 Target 814 CGTGTGTAAATATACAGCGCCGG 859 COR-23134-4 Target 815 TGTAAATATACAGCGCCGGCTGG 860 COR-23134-4 Target 816 GTAAATATACAGCGCCGGCTGGG 861 COR-23134-4 Target 817 GCGCCGGCTGGGTCAAGAGATGG 862 COR-23134-4 Target 818 GGCTGGGTCAAGAGATGGCTCGG 863 COR-23134-4 Target 819 GCTGGGTCAAGAGATGGCTCGGG 864 COR-23134-4 Target 820 TGGCTCGGGTGACGCGCGCGCGG 865 COR-23134-4 Target 821 GACGCGCGCGCGGCGTGTCCTGG 866 COR-23134-4 Target 822 CGCGCGGCGTGTCCTGGCGTTGG 867 COR-23134-4 Target 823 GCGTGTCCTGGCGTTGGCGCCGG 868 COR-23134-4 Target 824 CGTGTCCTGGCGTTGGCGCCGGG 869 COR-23134-4 Target 825 GTGTCCTGGCGTTGGCGCCGGGG 870 COR-23134-4 Target 826 AGCAACGATCGGCAGTAGTAAGG 871 COR-23134-4 Target 827 TGGGAGTAGAGCAACGCCCGTGG 872 COR-23134-4 Target 828 GAAGTTTGAAGCATCTTCCACGG 873 COR-23134-4 Target 829 AAGTTTGAAGCATCTTCCACGGG 874 COR-23134-4 Target 830 GATTTCGTGGTGAAACGAGATGG 875 COR-23134-4 Target 831 CTGAGATGCCTCTATGCGAAGGG 876 COR-23134-4 Target 832 GCTGAGATGCCTCTATGCGAAGG 877 COR-23134-4 Target 833 AACGTGGGTTTGGAGATGTACGG 878 COR-23134-4 Target 834 CTGAATCCAGAACGTGGGTTTGG 879 COR-23134-4 Target 835 GTTTGCTGAATCCAGAACGTGGG 880 COR-23134-4 Target 836 CGTTTGCTGAATCCAGAACGTGG 881 COR-23134-4 Target 837 ACATCTCCAAACCCACGTTCTGG 882 COR-23134-4 Target 838 CACGTTCTGGATTCAGCAAACGG 883 COR-23134-4 Target 839 ACAAATCCTAGTCAGTCCGCTGG 884 COR-23134-4 Target 840 AAACGGACGGCATATGCCAGCGG 885 COR-23134-4 Target 841 CATATGCCAGCGGACTGACTAGG 886 COR-23134-4 Target 842 AGCGGACTGACTAGGATTTGTGG 887 COR-23134-4 Target 843 TGTGACTGTGTTAGTTCCCTTGG 888 COR-23134-4 Target 844 CAAATACGAAATCATATCCAAGG 889 COR-23134-4 Target 845 AAATACGAAATCATATCCAAGGG 890 COR-23134-4 Target 846 CTAACACAGTCACAAACAACAGG 891 COR-23134-4 Target 847 GACAATTACAGCACAAGCGCAGG 892 COR-23134-4 Target 848 AATTACAGCACAAGCGCAGGAGG 893 COR-23134-4 Target 849 ATTACAGCACAAGCGCAGGAGGG 894 COR-23134-4 Target 850 TTTAACTGAACTAGGAAGAAAGG 895 COR-23134-4 Target 851 GTTATTGCTTCCACGACTATAGG 896 COR-23134-4 Target 852 CGAAAGCTGTCCTATAGTCGTGG 897 COR-23134-4 Target 853 GATAGGAATTCAGACACGAGAGG 898 COR-23134-4 Target 854 CGACCTTGAGAGAGCTCAGGAGG 899 COR-23134-4 Target 855 GTACGACCTTGAGAGAGCTCAGG 900 COR-23134-4 Target 856 AGGCCTCCTGAGCTCTCTCAAGG 901 COR-23134-4 Target 857 AGCTCTCTCAAGGTCGTACTCGG 902 COR-23134-4 Target 858 AAGGTCGTACTCGGCCTCGAAGG 903 COR-23134-4 Target 859 GTACTCGGCCTCGAAGGTCGCGG 904 COR-23134-4 Target 860 GGCCTCGAAGGTCGCGGTCACGG 905 COR-23134-4 Target 861 GCCTCGAAGGTCGCGGTCACGGG 906 COR-23134-4 Target 862 CCTCGAAGGTCGCGGTCACGGGG 907 COR-23134-4 Target 863 CGCAACTTCTCCGAGAACGCCGG 908 COR-23134-4 Target 864 GAAGCGGTCGATGATCACTCCGG 909 COR-23134-4 Target 865 GATGATCACTCCGGCGTTCTCGG 910 COR-23134-4 Target 866 CGGCGTTCTCGGAGAAGTTGCGG 911 COR-23134-4 Target 867 AGCGCGACTGGCAACGTCGTCGG 912 COR-23134-4 Target 868 AACGCCTTCACGAGCGCGACTGG 913 COR-23134-4 Target 869 GTTGCCAGTCGCGCTCGTGAAGG 914 COR-23134-4 Target 870 TCGCGCTCGTGAAGGCGTTGCGG 915 COR-23134-4 Target 871 AACCTGCAGTCCCGCAACTTCGG 916 COR-23134-4 Target 872 TCTCGAAGTAGCCGAAGTTGCGG 917 COR-23134-4 Target 873 CTCGAAGTAGCCGAAGTTGCGGG 918 COR-23134-4 Target 874 AGCCGAAGTTGCGGGACTGCAGG 919 COR-23134-4 Target 875 TGCGGGACTGCAGGTTGTCGAGG 920 COR-23134-4 Target 876 CAGGTTGTCGAGGCTAGTCGCGG 921 COR-23134-4 Target 877 GTTGTCGAGGCTAGTCGCGGTGG 922 COR-23134-4 Target 878 TCGAGGCTAGTCGCGGTGGCTGG 923 COR-23134-4 Target 879 AGTCGCGGTGGCTGGGACGATGG 924 COR-23134-4 Target 880 ATCCGCCTCTCCGTGAACTGGGG 925 COR-23134-4 Target 881 GATCCGCCTCTCCGTGAACTGGG 926 COR-23134-4 Target 882 CGATCCGCCTCTCCGTGAACTGG 927 COR-23134-4 Target 883 CAGTTCACGGAGAGGCGGATCGG 928 COR-23134-4 Target 884 TCGCGTAGCGCACTCTGACGCGG 929 COR-23134-4 Target 885 AGTTCAGGTCAACGAGCACTCGG 930 COR-23134-4 Target 886 TCGAGGTGCCGATCCAGTTCAGG 931 COR-23134-4 Target 887 GAGTGCTCGTTGACCTGAACTGG 932 COR-23134-4 Target 888 CTCGTTGACCTGAACTGGATCGG 933 COR-23134-4 Target 889 GGCAACAACATCCAGAACCGGGG 934 COR-23134-4 Target 890 CGGCAACAACATCCAGAACCGGG 935 COR-23134-4 Target 891 CCGGCAACAACATCCAGAACCGG 936 COR-23134-4 Target 892 TCGGCACCTCGATGTAGCCCCGG 937 COR-23134-4 Target 893 CTCGTCCGCCTCAACAACTCCGG 938 COR-23134-4 Target 894 CCGGTTCTGGATGTTGTTGCCGG 939 COR-23134-4 Target 895 TGTTGTTGCCGGAGTTGTTGAGG 940 COR-23134-4 Target 896 CGGAGTTGTTGAGGCGGACGAGG 941 COR-23134-4 Target 897 TCCGGACCAGGCTTCACCGGAGG 942 COR-23134-4 Target 898 ATCTCCGGACCAGGCTTCACCGG 943 COR-23134-4 Target 899 GAGGCGGACGAGGTCTCCTCCGG 944 COR-23134-4 Target 900 GGCCTTGTGATCTCCGGACCAGG 945 COR-23134-4 Target 901 AGGTCTCCTCCGGTGAAGCCTGG 946 COR-23134-4 Target 902 TTCAACGGCCTTGTGATCTCCGG 947 COR-23134-4 Target 903 TCCTCCGGTGAAGCCTGGTCCGG 948 COR-23134-4 Target 904 AGCCTGGTCCGGAGATCACAAGG 949 COR-23134-4 Target 905 ACCCAGATCCCGGCCGTCAAGGG 950 COR-23134-4 Target 906 CACCCAGATCCCGGCCGTCAAGG 951 COR-23134-4 Target 907 GAACATCATCACCCAGATCCCGG 952 COR-23134-4 Target 908 AGGAAGTTGCCCTTGACGGCCGG 953 COR-23134-4 Target 909 GGAAGTTGCCCTTGACGGCCGGG 954 COR-23134-4 Target 910 TGCCCTTGACGGCCGGGATCTGG 955 COR-23134-4 Target 911 GCCCTTGACGGCCGGGATCTGGG 956 COR-23134-4 Target 912 GGACGCACAGGTCAGCTGACCGG 957 COR-23134-4 Target 913 TCGTCGCGATGGTGTTCGTCCGG 958 COR-23134-4 Target 914 CCGTCTACTCCTGGACGCACAGG 959 COR-23134-4 Target 915 TCAGAGCTCCCGTCTACTCCTGG 960 COR-23134-4 Target 916 GTCAGCTGACCTGTGCGTCCAGG 961 COR-23134-4 Target 917 CTGTGCGTCCAGGAGTAGACGGG 962 COR-23134-4 Target 918 AACATCCGCCTCATCATCGGCGG 963 COR-23134-4 Target 919 TCGAACATCCGCCTCATCATCGG 964 COR-23134-4 Target 920 GCGTGCCGCCGATGATGAGGCGG 965 COR-23134-4 Target 921 TGATGAGGCGGATGTTCGACAGG 966 COR-23134-4 Target 922 TGAGGCGGATGTTCGACAGGCGG 967 COR-23134-4 Target 923 GGCGGATGTTCGACAGGCGGTGG 968 COR-23134-4 Target 924 GCGGATGTTCGACAGGCGGTGGG 969 COR-23134-4 Target 925 GTGGGAGTAGCTCTCGTAGTTGG 970 COR-23134-4 Target 926 TGGGAGTAGCTCTCGTAGTTGGG 971 COR-23134-4 Target 927 TCCCACCTGAGACCACCGAGAGG 972 COR-23134-4 Target 928 GCTCTCGTAGTTGGGCCTCTCGG 973 COR-23134-4 Target 929 CTCGTAGTTGGGCCTCTCGGTGG 974 COR-23134-4 Target 930 TTGGGCCTCTCGGTGGTCTCAGG 975 COR-23134-4 Target 931 GGCCTCTCGGTGGTCTCAGGTGG 976 COR-23134-4 Target 932 GCCTCTCGGTGGTCTCAGGTGGG 977 COR-23134-4 Target 933 GGTGGTCTCAGGTGGGAGCTCGG 978 COR-23134-4 Target 934 CGGTCTCGCTGTCGAAGAGCTGG 979 COR-23134-4 Target 935 GGTCTCGCTGTCGAAGAGCTGGG 980 COR-23134-4 Target 936 ACCATCGGCTACACCGGAGTCGG 981 COR-23134-4 Target 937 CTCTACACCATCGGCTACACCGG 982 COR-23134-4 Target 938 GAAGAGCTGGGTACCGACTCCGG 983 COR-23134-4 Target 939 GGATCGCTCCTCTACACCATCGG 984 COR-23134-4 Target 940 ACCGACTCCGGTGTAGCCGATGG 985 COR-23134-4 Target 941 CGGTGTAGCCGATGGTGTAGAGG 986 COR-23134-4 Target 942 AACCCTCTCAACTCCCTGCGCGG 987 COR-23134-4 Target 943 TGTAGAGGAGCGATCCGCGCAGG 988 COR-23134-4 Target 944 GTAGAGGAGCGATCCGCGCAGGG 989 COR-23134-4 Target 945 GATCCGCGCAGGGAGTTGAGAGG 990 COR-23134-4 Target 946 ATCCGCGCAGGGAGTTGAGAGGG 991 COR-23134-4 Target 947 CGTGGGCACGCTTCAACTGGAGG 992 COR-23134-4 Target 948 TCCCGTGGGCACGCTTCAACTGG 993 COR-23134-4 Target 949 GCCAGTGAACGGCGTCCCGTGGG 994 COR-23134-4 Target 950 CGCCAGTGAACGGCGTCCCGTGG 995 COR-23134-4 Target 951 CTCCAGTTGAAGCGTGCCCACGG 996 COR-23134-4 Target 952 TCCAGTTGAAGCGTGCCCACGGG 997 COR-23134-4 Target 953 CTGCTCACGACGCCAGTGAACGG 998 COR-23134-4 Target 954 GCCCACGGGACGCCGTTCACTGG 999 COR-23134-4 Target 955 CGTTCACTGGCGTCGTGAGCAGG 1000 COR-23134-4 Target 956 TACCGCACTGAGAGCTACGCTGG 1001 COR-23134-4 Target 957 TGCCAGCGTAGCTCTCAGTGCGG 1002 COR-23134-4 Target 958 GACGCTCCAGTTCACGAGCCGGG 1003 COR-23134-4 Target 959 TGACGCTCCAGTTCACGAGCCGG 1004 COR-23134-4 Target 960 TCTCAGTGCGGTAGACGTCCCGG 1005 COR-23134-4 Target 961 AGACGTCCCGGCTCGTGAACTGG 1006 COR-23134-4 Target 962 CACGAACACGTCCATCAACCCGG 1007 COR-23134-4 Target 963 CTCGTGAACTGGAGCGTCACCGG 1008 COR-23134-4 Target 964 TCGTGAACTGGAGCGTCACCGGG 1009 COR-23134-4 Target 965 CTGGAGCGTCACCGGGTTGATGG 1010 COR-23134-4 Target 966 CGGGTTGATGGACGTGTTCGTGG 1011 COR-23134-4 Target 967 ACCCTCAACACCTCGACCCACGG 1012 COR-23134-4 Target 968 TGGACGTGTTCGTGGCGCCGTGG 1013 COR-23134-4 Target 969 GGACGTGTTCGTGGCGCCGTGGG 1014 COR-23134-4 Target 970 GTTCGTGGCGCCGTGGGTCGAGG 1015 COR-23134-4 Target 971 CGCCGTGGGTCGAGGTGTTGAGG 1016 COR-23134-4 Target 972 GCCGTGGGTCGAGGTGTTGAGGG 1017 COR-23134-4 Target 973 CTCAACTTCAGGCCTATCGGCGG 1018 COR-23134-4 Target 974 CGCCTCAACTTCAGGCCTATCGG 1019 COR-23134-4 Target 975 GTGTTGAGGGTACCGCCGATAGG 1020 COR-23134-4 Target 976 TCGGCCACCGCCTCAACTTCAGG 1021 COR-23134-4 Target 977 CGCCGATAGGCCTGAAGTTGAGG 1022 COR-23134-4 Target 978 CGATAGGCCTGAAGTTGAGGCGG 1023 COR-23134-4 Target 979 TAGGCCTGAAGTTGAGGCGGTGG 1024 COR-23134-4 Target 980 CAGCACATGAACTACTGGGTCGG 1025 COR-23134-4 Target 981 CACGCAGCACATGAACTACTGGG 1026 COR-23134-4 Target 982 GCACGCAGCACATGAACTACTGG 1027 COR-23134-4 Target 983 GTAGTTCATGTGCTGCGTGCTGG 1028 COR-23134-4 Target 984 TCTACAGCGCCTCCAGCCGCTGG 1029 COR-23134-4 Target 985 TGTGCTGCGTGCTGGACCAGCGG 1030 COR-23134-4 Target 986 CTGCGTGCTGGACCAGCGGCTGG 1031 COR-23134-4 Target 987 CGTGCTGGACCAGCGGCTGGAGG 1032 COR-23134-4 Target 988 GCGGCTGGAGGCGCTGTAGATGG 1033 COR-23134-4 Target 989 CTGTAGATGGTGAGCTGCTCTGG 1034 COR-23134-4 Target 990 TGTAGATGGTGAGCTGCTCTGGG 1035 COR-23134-4 Target 991 CTTCCGCCCACCTCACCTGCTGG 1036 COR-23134-4 Target 992 GCTGCTCTGGGAAGTCCAGCAGG 1037 COR-23134-4 Target 993 TCTGGGAAGTCCAGCAGGTGAGG 1038 COR-23134-4 Target 994 GGGAAGTCCAGCAGGTGAGGTGG 1039 COR-23134-4 Target 995 AGTCCAGCAGGTGAGGTGGGCGG 1040 COR-23134-4 Target 996 GCGGAAGATTGCAGCTTCGATGG 1041 COR-23134-4 Target 997 GCTTCGATGGCGCTGAACGACGG 1042 COR-23134-4 Target 998 CCGGCTTCGCCTCCACCAACTGG 1043 COR-23134-4 Target 999 CGCGTTGTTGTTGAACCAGTTGG 1044 COR-23134-4 Target 1000 GTTGTTGTTGAACCAGTTGGTGG 1045 COR-23134-4 Target 1001 GGTCGCACGAACGCTCCATCCGG 1046 COR-23134-4 Target 1002 TGGAGCGTTCGTGCGACCGATGG 1047 COR-23134-4 Target 1003 GGAGCGTTCGTGCGACCGATGGG 1048 COR-23134-4 Target 1004 GAGCGTTCGTGCGACCGATGGGG 1049 COR-23134-4 Target 1005 GCTCACTCGTGAGATCTACACGG 1050 COR-23134-4 Target 1006 AGCTGCGCGCTCGTGTTGATCGG 1051 COR-23134-4 Target 1007 GCTGCGCGCTCGTGTTGATCGGG 1052 COR-23134-4 Target 1008 TGTTCCCGAGCTACGACACGCGG 1053 COR-23134-4 Target 1009 TAGATCCGCGTGTCGTAGCTCGG 1054 COR-23134-4 Target 1010 AGATCCGCGTGTCGTAGCTCGGG 1055 COR-23134-4 Target 1011 AGCTCGGGAACAGCGCGACGAGG 1056 COR-23134-4 Target 1012 GGAACAGCGCGACGAGGTCGAGG 1057 COR-23134-4 Target 1013 TTCCGCAGGGACCTCACACTCGG 1058 COR-23134-4 Target 1014 GGTCGAGGACTCCGAGTGTGAGG 1059 COR-23134-4 Target 1015 CCGCTACAACCAGTTCCGCAGGG 1060 COR-23134-4 Target 1016 TCCGCTACAACCAGTTCCGCAGG 1061 COR-23134-4 Target 1017 CTCCGAGTGTGAGGTCCCTGCGG 1062 COR-23134-4 Target 1018 GTGTGAGGTCCCTGCGGAACTGG 1063 COR-23134-4 Target 1019 CCCTGCGGAACTGGTTGTAGCGG 1064 COR-23134-4 Target 1020 GCGGCACGAACGCTGAGAGCTGG 1065 COR-23134-4 Target 1021 ACCGGCCTGAACAACCTGCGCGG 1066 COR-23134-4 Target 1022 TCTCAGCGTTCGTGCCGCGCAGG 1067 COR-23134-4 Target 1023 TCGTGCCGCGCAGGTTGTTCAGG 1068 COR-23134-4 Target 1024 TGCGCACGCTGGTACAACACCGG 1069 COR-23134-4 Target 1025 GCCGCGCAGGTTGTTCAGGCCGG 1070 COR-23134-4 Target 1026 ACAGCGACTACTGCGCACGCTGG 1071 COR-23134-4 Target 1027 GCGCCAGGCGGAGAAGACCCGGG 1072 COR-23134-4 Target 1028 AGCGCCAGGCGGAGAAGACCCGG 1073 COR-23134-4 Target 1029 CGCAGTAGTCGCTGTACTCCCGG 1074 COR-23134-4 Target 1030 GCAGTAGTCGCTGTACTCCCGGG 1075 COR-23134-4 Target 1031 GCGCTACTACGAGCGCCAGGCGG 1076 COR-23134-4 Target 1032 CCAGCGCTACTACGAGCGCCAGG 1077 COR-23134-4 Target 1033 ACTCCCGGGTCTTCTCCGCCTGG 1078 COR-23134-4 Target 1034 CCTGGCGCTCGTAGTAGCGCTGG 1079 COR-23134-4 Target 1035 CGAGTTCGGACTCACGTCGCAGG 1080 COR-23134-4 Target 1036 AGCCTGTTCGGCAGCGAGTTCGG 1081 COR-23134-4 Target 1037 CTCAGAGACGCTAGCCTGTTCGG 1082 COR-23134-4 Target 1038 GTCCGAACTCGCTGCCGAACAGG 1083 COR-23134-4 Target 1039 CGAACAGGCTAGCGTCTCTGAGG 1084 COR-23134-4 Target 1040 GGCTAGCGTCTCTGAGGAGCAGG 1085 COR-23134-4 Target 1041 TAGCGTCTCTGAGGAGCAGGAGG 1086 COR-23134-4 Target 1042 CGTCTCTGAGGAGCAGGAGGTGG 1087 COR-23134-4 Target 1043 GGAGGTGGAGGTTGGCAGCTTGG 1088 COR-23134-4 Target 1044 CAGCTTGGGCGTAGACCATGAGG 1089 COR-23134-4 Target 1045 TGGGCGTAGACCATGAGGAGCGG 1090 COR-23134-4 Target 1046 CTTCGCCATCAACAACCAGCAGG 1091 COR-23134-4 Target 1047 CCATGAGGAGCGGCACCTGCTGG 1092 COR-23134-4 Target 1048 TGGTTGTTGATGGCGAAGAGTGG 1093 COR-23134-4 Target 1049 CGAAGAGTGGCATCGCGTTCAGG 1094 COR-23134-4 Target 1050 CTACACCCAGTACATCGCGCTGG 1095 COR-23134-4 Target 1051 CGAGCTCCAGCGCGATGTACTGG 1096 COR-23134-4 Target 1052 GAGCTCCAGCGCGATGTACTGGG 1097 COR-23134-4 Target 1053 GCGCGATGTACTGGGTGTAGAGG 1098 COR-23134-4 Target 1054 ACCGCGACAACGCGAGGACCCGG 1099 COR-23134-4 Target 1055 ACTGGGTGTAGAGGACGCTCCGG 1100 COR-23134-4 Target 1056 CTGGGTGTAGAGGACGCTCCGGG 1101 COR-23134-4 Target 1057 TCGAGAACCGCGACAACGCGAGG 1102 COR-23134-4 Target 1058 TCCGGGTCCTCGCGTTGTCGCGG 1103 COR-23134-4 Target 1059 ACCAGCAGTCCCTCGAGGACTGG 1104 COR-23134-4 Target 1060 AGCCTACCAGCAGTCCCTCGAGG 1105 COR-23134-4 Target 1061 GGTTCTCGAGCCAGTCCTCGAGG 1106 COR-23134-4 Target 1062 GTTCTCGAGCCAGTCCTCGAGGG 1107 COR-23134-4 Target 1063 GCCAGTCCTCGAGGGACTGCTGG 1108 COR-23134-4 Target 1064 CTGCTGGTAGGCTCTGAAGCTGG 1109 COR-23134-4 Target 1065 CTCGCCAGACTCCAAGGCCTCGG 1110 COR-23134-4 Target 1066 AGGCTCTGAAGCTGGCTCCGAGG 1111 COR-23134-4 Target 1067 ACGGCTCTCGCCAGACTCCAAGG 1112 COR-23134-4 Target 1068 TGAAGCTGGCTCCGAGGCCTTGG 1113 COR-23134-4 Target 1069 GGCTCCGAGGCCTTGGAGTCTGG 1114 COR-23134-4 Target 1070 CACGATGAACGCTCGCAACACGG 1115 COR-23134-4 Target 1071 AGCACGTCGAGCAGCTGGTCAGG 1116 COR-23134-4 Target 1072 CATGGAGCACGTCGAGCAGCTGG 1117 COR-23134-4 Target 1073 CGATCCCTGGGAGATCTTCATGG 1118 COR-23134-4 Target 1074 GCCATCAGGTCGCGATCCCTGGG 1119 COR-23134-4 Target 1075 GGCCATCAGGTCGCGATCCCTGG 1120 COR-23134-4 Target 1076 GTGCTCCATGAAGATCTCCCAGG 1121 COR-23134-4 Target 1077 TGCTCCATGAAGATCTCCCAGGG 1122 COR-23134-4 Target 1078 GTCGGCGAGCTCTGGCCATCAGG 1123 COR-23134-4 Target 1079 TCCCAGGGATCGCGACCTGATGG 1124 COR-23134-4 Target 1080 AGCTTCTACAGCTTCATCGTCGG 1125 COR-23134-4 Target 1081 AGTCCCATTCGCAGGTCAGCTGG 1126 COR-23134-4 Target 1082 GTCCTCGGAGTCCCATTCGCAGG 1127 COR-23134-4 Target 1083 CTCGCCAGCTGACCTGCGAATGG 1128 COR-23134-4 Target 1084 TCGCCAGCTGACCTGCGAATGGG 1129 COR-23134-4 Target 1085 GGTCGCATACTCGGCGTCCTCGG 1130 COR-23134-4 Target 1086 GACCTGCGAATGGGACTCCGAGG 1131 COR-23134-4 Target 1087 AACATCGCCGGTCGCATACTCGG 1132 COR-23134-4 Target 1088 CAGACTGGCATCAACATCGCCGG 1133 COR-23134-4 Target 1089 GAGGACGCCGAGTATGCGACCGG 1134 COR-23134-4 Target 1090 AGCGCCTCGACCGTGCAGACTGG 1135 COR-23134-4 Target 1091 GATGTTGATGCCAGTCTGCACGG 1136 COR-23134-4 Target 1092 GATGCCAGTCTGCACGGTCGAGG 1137 COR-23134-4 Target 1093 ACGGTCGAGGCGCTAACGAGCGG 1138 COR-23134-4 Target 1094 CGGTCGAGGCGCTAACGAGCGGG 1139 COR-23134-4 Target 1095 CGGGTTGATGTTGTTGCCCTCGG 1140 COR-23134-4 Target 1096 CTCACTGGACGCTCGCATCGAGG 1141 COR-23134-4 Target 1097 CGCGCAGATGGACCTCTCACTGG 1142 COR-23134-4 Target 1098 CGATGCGAGCGTCCAGTGAGAGG 1143 COR-23134-4 Target 1099 CTCCAACCACTCCGCGCAGATGG 1144 COR-23134-4 Target 1100 CAGTGAGAGGTCCATCTGCGCGG 1145 COR-23134-4 Target 1101 AGAGGTCCATCTGCGCGGAGTGG 1146 COR-23134-4 Target 1102 GTCCATCTGCGCGGAGTGGTTGG 1147 COR-23134-4 Target 1103 CTGCGCGGAGTGGTTGGAGACGG 1148 COR-23134-4 Target 1104 GCGGAGTGGTTGGAGACGGCTGG 1149 COR-23134-4 Target 1105 CGGAGTGGTTGGAGACGGCTGGG 1150 COR-23134-4 Target 1106 TGGAGACGGCTGGGATCGACAGG 1151 COR-23134-4 Target 1107 GGAGACGGCTGGGATCGACAGGG 1152 COR-23134-4 Target 1108 TTATCTCGTTCTCGTTCTTGCGG 1153 COR-23134-4 Target 1109 CTCGTTCTCGTTCTTGCGGTTGG 1154 COR-23134-4 Target 1110 GTTCTCGTTCTTGCGGTTGGAGG 1155 COR-23134-4 Target 1111 TTCTCGTTCTTGCGGTTGGAGGG 1156 COR-23134-4 Target 1112 GTTCTTGCGGTTGGAGGGCATGG 1157 COR-23134-4 Target 1113 CTTGCGGTTGGAGGGCATGGCGG 1158 COR-23134-4 Target 1114 TTGGAGGGCATGGCGGATCCCGG 1159 COR-23134-4 Target 1115 CACACTTGGACAATCTAGAGCGG 1160 COR-23134-4 Target 1116 GCCCTCTAGATGCATGCTCGAGG 1161 COR-23134-4 Target 1117 CGCCTCGAGCATGCATCTAGAGG 1162 COR-23134-4 Target 1118 GCCTCGAGCATGCATCTAGAGGG 1163 COR-23134-4 Target 1119 TCACTTAGGTGACCAAGCTTCGG 1164 COR-23134-4 Target 1120 GCATCTAGAGGGCCGAAGCTTGG 1165 COR-23134-4 Target 1121 TCACGTGACCCTAGTCACTTAGG 1166 COR-23134-4 Target 1122 GCTTGGTCACCTAAGTGACTAGG 1167 COR-23134-4 Target 1123 TGCCCGGGAATAAGTGACTAGGG 1168 COR-23134-4 Target 1124 GTGCCCGGGAATAAGTGACTAGG 1169 COR-23134-4 Target 1125 TACAAGAAAGCTGGGTGCCCGGG 1170 COR-23134-4 Target 1126 GTACAAGAAAGCTGGGTGCCCGG 1171 COR-23134-4 Target 1127 TGACCCTAGTCACTTATTCCCGG 1172 COR-23134-4 Target 1128 GACCCTAGTCACTTATTCCCGGG 1173 COR-23134-4 Target 1129 GCCACTTTGTACAAGAAAGCTGG 1174 COR-23134-4 Target 1130 CCCAGCTTTCTTGTACAAAGTGG 1175 COR-23134-4 Target 1131 AGAGTCGACAGATCCGTTAACGG 1176 COR-23134-4 Target 1132 TTGTACAAAGTGGCCGTTAACGG 1177 COR-23134-4 Target 1133 GGATCTGTCGACTCTAGACCCGG 1178 COR-23134-4 Target 1134 GATCTGTCGACTCTAGACCCGGG 1179 COR-23134-4 Target 1135 ACTCTAGACCCGGGCTAATTAGG 1180 COR-23134-4 Target 1136 CTCTAGACCCGGGCTAATTAGGG 1181 COR-23134-4 Target 1137 TCTAGACCCGGGCTAATTAGGGG 1182 COR-23134-4 Target 1138 CTAGACCCGGGCTAATTAGGGGG 1183 COR-23134-4 Target 1139 CCGGAATCGGCCGTACGTAAGGG 1184 COR-23134-4 Target 1140 GCCGGAATCGGCCGTACGTAAGG 1185 COR-23134-4 Target 1141 GCTAAGCACTAGGCCGGAATCGG 1186 COR-23134-4 Target 1142 CCGTTAGCTAAGCACTAGGCCGG 1187 COR-23134-4 Target 1143 CCCTTACGTACGGCCGATTCCGG 1188 COR-23134-4 Target 1144 TTTCCCGTTAGCTAAGCACTAGG 1189 COR-23134-4 Target 1145 CCGGCCTAGTGCTTAGCTAACGG 1190 COR-23134-4 Target 1146 CGGCCTAGTGCTTAGCTAACGGG 1191 COR-23134-4 Target 1147 CCACTAACTAATCGTTAAGCGGG 1192 COR-23134-4 Target 1148 TCCACTAACTAATCGTTAAGCGG 1193 COR-23134-4 Target 1149 CCCGCTTAACGATTAGTTAGTGG 1194 COR-23134-4 Target 1150 CTTAACGATTAGTTAGTGGACGG 1195 COR-23134-4 Target 1151 TTAACGATTAGTTAGTGGACGGG 1196 COR-23134-4 Target 1152 TAACGATTAGTTAGTGGACGGGG 1197 COR-23134-4 Target 1153 CCACCTGAATTCCAGCACACTGG 1198 COR-23134-4 Target 1154 GCGCCAGTGTGCTGGAATTCAGG 1199 COR-23134-4 Target 1155 AAAGAGGCCTAAGCAACATAAGG 1200 COR-23134-4 Target 1156 GGTGGTACCTTATGTTGCTTAGG 1201 COR-23134-4 Target 1157 TGGTTCCATCTCAAATTCCGTGG 1202 COR-23134-4 Target 1158 GCCAAGAAATCATGTACAACTGG 1203 COR-23134-4 Target 1159 ACCAGTTGTACATGATTTCTTGG 1204 COR-23134-4 Target 1160 AACTAACAGTAGATCGTCTCTGG 1205 COR-23134-4 Target 1161 GCAAGCATAAGCATGTAGATTGG 1206 COR-23134-4 Target 1162 CATGCTTATGCTTGCCTGAATGG 1207 COR-23134-4 Target 1163 CTGCGCTTCTTTCTTGTTAGTGG 1208 COR-23134-4 Target 1164 TCTTGTTAGTGGTTCATGCATGG 1209 COR-23134-4 Target 1165 GTACGTATTAAGCATGTAGAAGG 1210 COR-23134-4 Target 1166 CAGTGCCGTTTAGGCAAGTATGG 1211 COR-23134-4 Target 1167 GTACTCCATACTTGCCTAAACGG 1212 COR-23134-4 Target 1168 TTCTATCAGATCGCAACTCTTGG 1213 COR-23134-4 Target 1169 ACTGCTGCTAATCTGTGTCTAGG 1214 COR-23134-4 Target 1170 AAATTTGGACATGCTCTGCTCGG 1215 COR-23134-4 Target 1171 TGCTGTAGTTTCACCGTTGCTGG 1216 COR-23134-4 Target 1172 AAATGCTTCTTCACCAGCAACGG 1217 COR-23134-4 Target 1173 TTCAAACTCATGGGCACGAGTGG 1218 COR-23134-4 Target 1174 CACCTGTTGTTCAAACTCATGGG 1219 COR-23134-4 Target 1175 CCACCTGTTGTTCAAACTCATGG 1220 COR-23134-4 Target 1176 TGCCCATGAGTTTGAACAACAGG 1221 COR-23134-4 Target 1177 CCATGAGTTTGAACAACAGGTGG 1222 COR-23134-4 Target 1178 TGAACAACAGGTGGGCGTTGCGG 1223 COR-23134-4 Target 1179 GGTGGGCGTTGCGGCGCCTACGG 1224 COR-23134-4 Target 1180 ATATCTACATGGATTACACCAGG 1225 COR-23134-4 Target 1181 ACTCCAATCCCTCAGCATCCTGG 1226 COR-23134-4 Target 1182 AGTCCAGGATGCTGAGGGATTGG 1227 COR-23134-4 Target 1183 GGAAACTTGCAGGACTAACTTGG 1228 COR-23134-4 Target 1184 TTGTGGAATCGGAAACTTGCAGG 1229 COR-23134-4 Target 1185 ATTTCAATTAGTTGTGGAATCGG 1230 COR-23134-4 Target 1186 TCATCTATTTCAATTAGTTGTGG 1231 COR-23134-4 Target 1187 GGCTGTTAGTTTCAATGCAATGG 1232 COR-23134-4 Target 1188 AACAGCCAATAAGCTTCCCTTGG 1233 COR-23134-4 Target 1189 CCGGCGTCAGTGTGTTCTGGAGG 1234 COR-23134-4 Target 1190 GGGCCGGCGTCAGTGTGTTCTGG 1235 COR-23134-4 Target 1191 TCTTATCCTATGAGACGGGCCGG 1236 COR-23134-4 Target 1192 CCTCCAGAACACACTGACGCCGG 1237 COR-23134-4 Target 1193 CCCGTCTTATCCTATGAGACGGG 1238 COR-23134-4 Target 1194 ACCCGTCTTATCCTATGAGACGG 1239 COR-23134-4 Target 1195 CTGACGCCGGCCCGTCTCATAGG 1240 COR-23134-4 Target 1196 GCCCGTCTCATAGGATAAGACGG 1241 COR-23134-4 Target 1197 CCCGTCTCATAGGATAAGACGGG 1242 COR-23134-4 Target 1198 GAGTTAACTTGCCAGATCCTCGG 1243 COR-23134-4 Target 1199 GACGGGTTATTTGTACACCGAGG 1244 COR-23134-4 Target 1200 TTATTTGTACACCGAGGATCTGG 1245 COR-23134-4 Target 1201 TAAGCTGAACTGCAGAATTCAGG 1246 COR-23134-4 Target 1202 AGAAAGCTTAATTACATTAAAGG 1247 COR-23134-4 Target 1203 AGAAGTATTACTACGATTGGTGG 1248 COR-23134-4 Target 1204 TGCAAGATTCTCTCTTCCTCTGG 1249 COR-23134-4 Target 1205 GTTAATACACATAACTTACTTGG 1250 COR-23134-4 Target 1206 CATAACTTACTTGGTAAGCTTGG 1251 COR-23134-4 Target 1207 AGACTCACTAGAAATTGCAAAGG 1252 COR-23134-4 Target 1208 ACAGAAGACAATTCAAGACTTGG 1253 COR-23134-4 Target 1209 CAGAAGACAATTCAAGACTTGGG 1254 COR-23134-4 Target 1210 ACACTATTGCACACAAAGAGTGG 1255 COR-23134-4 Target 1211 TGGCAAACAAGCGGCGCCCTTGG 1256 COR-23134-4 Target 1212 AACAAGCGGCGCCCTTGGTTTGG 1257 COR-23134-4 Target 1213 ATAGTAAGAGACAATTGTTTTGG 1258 COR-23134-4 Target 1214 GCTATGTATGTTAAACATTATGG 1259 COR-23134-4 Target 1215 TTCGTTGTGATGCAGTAGTACGG 1260 COR-23134-4 Target 1216 GATATACTACTGGTGAAGATGGG 1261 COR-23134-4 Target 1217 TGATATACTACTGGTGAAGATGG 1262 COR-23134-4 Target 1218 TTTCGGCTTTGATATACTACTGG 1263 COR-23134-4 Target 1219 CCTTCGTTTGGGCTTGTAACGGG 1264 COR-23134-4 Target 1220 ACCTTCGTTTGGGCTTGTAACGG 1265 COR-23134-4 Target 1221 CCCGTTACAAGCCCAAACGAAGG 1266 COR-23134-4 Target 1222 GGGAGGTGATCTATTTCACCTGG 1267 COR-23134-4 Target 1223 ACGGAAACACAAACTACACCAGG 1268 COR-23134-4 Target 1224 TCGTGTCTCATGCACTTGGGAGG 1269 COR-23134-4 Target 1225 GGTTCGTGTCTCATGCACTTGGG 1270 COR-23134-4 Target 1226 TGGTTCGTGTCTCATGCACTTGG 1271 COR-23134-4 Target 1227 GTGATCTGATGATAAGTGGTTGG 1272 COR-23134-4 Target 1228 AGTTGTGATCTGATGATAAGTGG 1273 COR-23134-4 Target 1229 GCATGCATCTAGACGTACGTCGG 1274 COR-23134-4 Target 1230 CATGCATCTAGACGTACGTCGGG 1275 COR-23134-4 Target 1231 CGCTAGTCACTGATTAGAGACGG 1276 COR-23134-4 Target 1232 AGACGGTCTGCACTAACTAACGG 1277 COR-23134-4 Target 1233 TCAATCGAGTACGATGTCGGAGG 1278 COR-23134-4 Target 1234 ACGTCAATCGAGTACGATGTCGG 1279 COR-23134-4 Target 1235 GACGTCGCGAGAGCTCCCTAAGG 1280 COR-23134-4 Target 1236 ACGTCGCGAGAGCTCCCTAAGGG 1281 COR-23134-4 Target 1237 CGTCGCGAGAGCTCCCTAAGGGG 1282 COR-23134-4 Target 1238 GCGCCCGGACCGGGCCATCTAGG 1283 COR-23134-4 Target 1239 TGCAGGGTCGCGCCCGGACCGGG 1284 COR-23134-4 Target 1240 CTGCAGGGTCGCGCCCGGACCGG 1285 COR-23134-4 Target 1241 TTAACCTGCAGGGTCGCGCCCGG 1286 COR-23134-4 Target 1242 GGGCCTAGATGGCCCGGTCCGGG 1287 COR-23134-4 Target 1243 GATAGCAGCATTAACCTGCAGGG 1288 COR-23134-4 Target 1244 GGATAGCAGCATTAACCTGCAGG 1289 COR-23134-4 Target 1245 CGGTCCGGGCGCGACCCTGCAGG 1290 COR-23134-4 Target 1246 CACCGAATTTCTCCACCTGTTGG 1291 COR-23134-4 Target 1247 GGTTAATGCTGCTATCCAACAGG 1292 COR-23134-4 Target 1248 TAATGCTGCTATCCAACAGGTGG 1293 COR-23134-4 Target 1249 ATCCAACAGGTGGAGAAATTCGG 1294 COR-23134-4 Target 1250 GGTGGAGAAATTCGGTGAACAGG 1295 COR-23134-4 Target 1251 GAAATTCGGTGAACAGGGTAGGG 1296 COR-23134-4 Target 1252 TTTGTTCATCTATAGGCTCTCGG 1297 COR-23134-4 Target 1253 GTTCATCTATAGGCTCTCGGCGG 1298 COR-23134-4 Target 1254 TAGCTTAGATAACAGATGGTCGG 1299 COR-23134-4 Target 1255 ATGATAGCTTAGATAACAGATGG 1300 COR-23134-4 Target 1256 CATTCATTTATAGCATATGTTGG 1301 COR-23134-4 Target 1257 ACGACACAAAGGAATTGGTAAGG 1302 COR-23134-4 Target 1258 AGCATACTGCAACGACACAAAGG 1303 COR-23134-4 Target 1259 TGCAGTATGCTGTAGTGTATCGG 1304 COR-23134-4 Target 1260 ACACAACAACACTCGAGTCTGGG 1305 COR-23134-4 Target 1261 AACACAACAACACTCGAGTCTGG 1306 COR-23134-4 Target 1262 GTGTTCTGTCTTCTGTTGCTTGG 1307 COR-23134-4 Target 1263 TTATAGTATTCTCTCTCGTGTGG 1308 COR-23134-4 Target 1264 GTATTCTCTCTCGTGTGGCTTGG 1309 COR-23134-4 Target 1265 TGTTTGAGTGGCCAGCAAACTGG 1310 COR-23134-4 Target 1266 GGCTTGGTCAGCCAGTTTGCTGG 1311 COR-23134-4 Target 1267 CAACTCGAGTGTTGTTTGAGTGG 1312 COR-23134-4 Target 1268 CAACACTCGAGTTGTACATATGG 1313 COR-23134-4 Target 1269 TATGGTTCTGTTGTACATGGTGG 1314 COR-23134-4 Target 1270 TGGTGGTGATAGAGCATTACGGG 1315 COR-23134-4 Target 1271 ACTGTCGTTGCATTGCCATGAGG 1316 COR-23134-4 Target 1272 CCATCTCACTAAATTCTCCTTGG 1317 COR-23134-4 Target 1273 TTGCATTGCCATGAGGACCAAGG 1318 COR-23134-4 Target 1274 CCAAGGAGAATTTAGTGAGATGG 1319 COR-23134-4 Target 1275 ATAGCTTCTGAAATCGCAGAAGG 1320 COR-23134-4 Target 1276 TAAATTCATTCAGACTGTTTTGG 1321 COR-23134-4 Target 1277 AGCAAAGGCTGCGTTACATAAGG 1322 COR-23134-4 Target 1278 GTAATACTAGTTTGTAGCAAAGG 1323 COR-23134-4 Target 1279 ATCCATGTGTTGACTGTTGAGGG 1324 COR-23134-4 Target 1280 CATCCATGTGTTGACTGTTGAGG 1325 COR-23134-4 Target 1281 AACAGTCAACACATGGATGATGG 1326 COR-23134-4 Target 1282 AGTACGTATGAGTGAGGTCTAGG 1327 COR-23134-4 Target 1283 AATTGACTGGCGGCAAGAGGAGG 1328 COR-23134-4 Target 1284 ACAAATTGACTGGCGGCAAGAGG 1329 COR-23134-4 Target 1285 CCATTAGACAAATTGACTGGCGG 1330 COR-23134-4 Target 1286 CGGCCATTAGACAAATTGACTGG 1331 COR-23134-4 Target 1287 CCGCCAGTCAATTTGTCTAATGG 1332 COR-23134-4 Target 1288 TGCCATCACTTTCCATGTTACGG 1333 COR-23134-4 Target 1289 TTTGTCTAATGGCCGTAACATGG 1334 COR-23134-4 Target 1290 GGCCGTAACATGGAAAGTGATGG 1335 COR-23134-4 Target 1291 GAAAGTGATGGCAATCTCAGTGG 1336 COR-23134-4 Target 1292 AGTGATGGCAATCTCAGTGGTGG 1337 COR-23134-4 Target 1293 GGCAATCTCAGTGGTGGAGTCGG 1338 COR-23134-4 Target 1294 GCAATCTCAGTGGTGGAGTCGGG 1339 COR-23134-4 Target 1295 GGGAGCTGTAGCTGTGGGAAAGG 1340 COR-23134-4 Target 1296 GCGTCCGCAGCGGAGAATAAAGG 1341 COR-23134-4 Target 1297 ACGTCTGTCTGCGTCCGCAGCGG 1342 COR-23134-4 Target 1298 TAATCCTTTATTCTCCGCTGCGG 1343 COR-23134-4 Target 1299 TGACACCGTTCACTCACTGGAGG 1344 COR-23134-4 Target 1300 AGATGACACCGTTCACTCACTGG 1345 COR-23134-4 Target 1301 CGTAGCCTCCAGTGAGTGAACGG 1346 COR-23134-4 Target 1302 TGTCATCTGCATGAATGTGTTGG 1347 COR-23134-4 Target 1303 CACTCTTACGAACGAGCCCTCGG 1348 COR-23134-4 Target 1304 TGTGTTGGCAAACTGTTCCGAGG 1349 COR-23134-4 Target 1305 GTGTTGGCAAACTGTTCCGAGGG 1350 COR-23134-4 Target 1306 CGAGGGCTCGTTCGTAAGAGTGG 1351 COR-23134-4 Target 1307 TACGCGTACCGCCTCGACACTGG 1352 COR-23134-4 Target 1308 GAGTGGCTTCTCCAGTGTCGAGG 1353 COR-23134-4 Target 1309 TGTCGAGGCGGTACGCGTAATGG 1354 COR-23134-4 Target 1310 TACGCGTAATGGTAGTTGAGCGG 1355 COR-23134-4 Target 1311 GGTGATGACCTTTCAGGCAGCGG 1356 COR-23134-4 Target 1312 GGAGGAGGTGATGACCTTTCAGG 1357 COR-23134-4 Target 1313 GAGCGGAACCGCTGCCTGAAAGG 1358 COR-23134-4 Target 1314 CCATGATCGCAGAAGGGAGGAGG 1359 COR-23134-4 Target 1315 CTTCCATGATCGCAGAAGGGAGG 1360 COR-23134-4 Target 1316 TGTCTTCCATGATCGCAGAAGGG 1361 COR-23134-4 Target 1317 CTGTCTTCCATGATCGCAGAAGG 1362 COR-23134-4 Target 1318 CCTCCTCCCTTCTGCGATCATGG 1363 COR-23134-4 Target 1319 TCTGCGATCATGGAAGACAGTGG 1364 COR-23134-4 Target 1320 ACCATTTACATCCTCCTGCAGGG 1365 COR-23134-4 Target 1321 GACCATTTACATCCTCCTGCAGG 1366 COR-23134-4 Target 1322 GGAAGACAGTGGAGCCCTGCAGG 1367 COR-23134-4 Target 1323 AGACAGTGGAGCCCTGCAGGAGG 1368 COR-23134-4 Target 1324 GCCCTGCAGGAGGATGTAAATGG 1369 COR-23134-4 Target 1325 ACCAGCCTGCCACCCAGACGGGG 1370 COR-23134-4 Target 1326 CACCAGCCTGCCACCCAGACGGG 1371 COR-23134-4 Target 1327 CCACCAGCCTGCCACCCAGACGG 1372 COR-23134-4 Target 1328 CCGTCTGGGTGGCAGGCTGGTGG 1373 COR-23134-4 Target 1329 TTTGTGCAGGAGGCGAGCGGTGG 1374 COR-23134-4 Target 1330 CACTTTGTGCAGGAGGCGAGCGG 1375 COR-23134-4 Target 1331 GGAGATGCACTTTGTGCAGGAGG 1376 COR-23134-4 Target 1332 CCTGGAGATGCACTTTGTGCAGG 1377 COR-23134-4 Target 1333 TCGAGTGCGGATTCGGCACCTGG 1378 COR-23134-4 Target 1334 GCTGGACTCGAGTGCGGATTCGG 1379 COR-23134-4 Target 1335 CTTCCAGCTGGACTCGAGTGCGG 1380 COR-23134-4 Target 1336 TGTCCAGTTTCCCTTCCAGCTGG 1381 COR-23134-4 Target 1337 AATCCGCACTCGAGTCCAGCTGG 1382 COR-23134-4 Target 1338 CGCACTCGAGTCCAGCTGGAAGG 1383 COR-23134-4 Target 1339 GCACTCGAGTCCAGCTGGAAGGG 1384 COR-23134-4 Target 1340 AGTCCAGCTGGAAGGGAAACTGG 1385 COR-23134-4 Target 1341 TTTGAGATTCCTGTGCAAGGTGG 1386 COR-23134-4 Target 1342 GAATTTGAGATTCCTGTGCAAGG 1387 COR-23134-4 Target 1343 CTGGACATGCCACCTTGCACAGG 1388 COR-23134-4 Target 1344 GAGCTTGCTTCTAGACGGTGGGG 1389 COR-23134-4 Target 1345 TGAGCTTGCTTCTAGACGGTGGG 1390 COR-23134-4 Target 1346 GTGAGCTTGCTTCTAGACGGTGG 1391 COR-23134-4 Target 1347 CCTGTGAGCTTGCTTCTAGACGG 1392 COR-23134-4 Target 1348 CCGTCTAGAAGCAAGCTCACAGG 1393 COR-23134-4 Target 1349 ACGAGCAGGCATGTAGTGAAAGG 1394 COR-23134-4 Target 1350 TGCCTGCTCGTGAAGTCCGATGG 1395 COR-23134-4 Target 1351 GCCTGCTCGTGAAGTCCGATGGG 1396 COR-23134-4 Target 1352 CCTGCTCGTGAAGTCCGATGGGG 1397 COR-23134-4 Target 1353 CTGCTCGTGAAGTCCGATGGGGG 1398 COR-23134-4 Target 1354 TGTCAGGATTAGCTCAGTTAGGG 1399 COR-23134-4 Target 1355 CTGTCAGGATTAGCTCAGTTAGG 1400 COR-23134-4 Target 1356 CACGAGAGTGCTGCACTGTCAGG 1401 COR-23134-4 Target 1357 CTCGTGCGTGATGAGCAAGTTGG 1402 COR-23134-4 Target 1358 GCCGCTGAGAATGGATACTGTGG 1403 COR-23134-4 Target 1359 CACTGCATGGCCGCTGAGAATGG 1404 COR-23134-4 Target 1360 GCCACAGTATCCATTCTCAGCGG 1405 COR-23134-4 Target 1361 CGCCCACGCAGCTCACTGCATGG 1406 COR-23134-4 Target 1362 CGGCCATGCAGTGAGCTGCGTGG 1407 COR-23134-4 Target 1363 GGCCATGCAGTGAGCTGCGTGGG 1408 COR-23134-4 Target 1364 TGAGCTGCGTGGGCGTTAGAAGG 1409 COR-23134-4 Target 1365 CATAATGCGCATCCAGAATGCGG 1410 COR-23134-4 Target 1366 TCTGGATGCGCATTATGTTGAGG 1411 COR-23134-4 Target 1367 GGACTACGAGGATACTGATAGGG 1412 COR-23134-4 Target 1368 CGGACTACGAGGATACTGATAGG 1413 COR-23134-4 Target 1369 AGATGGATATGCGGACTACGAGG 1414 COR-23134-4 Target 1370 TGGCAATACAGATGGATATGCGG 1415 COR-23134-4 Target 1371 TTTGGAGATGGCAATACAGATGG 1416 COR-23134-4 Target 1372 TCTTACCAACTATTTGGAGATGG 1417 COR-23134-4 Target 1373 TGCTAATCTTACCAACTATTTGG 1418 COR-23134-4 Target 1374 TATTGCCATCTCCAAATAGTTGG 1419 COR-23134-4 Target 1375 AAATAGTTGGTAAGATTAGCAGG 1420 COR-23134-4 Target 1376 TCAAAGCCACGCAGACCGGCTGG 1421 COR-23134-4 Target 1377 TAGCTCAAAGCCACGCAGACCGG 1422 COR-23134-4 Target 1378 CAGGTTGAATCGCTGCCAGCCGG 1423 COR-23134-4 Target 1379 TCGCTGCCAGCCGGTCTGCGTGG 1424 COR-23134-4 Target 1380 CTGGGTGCAAGGCTTGTTGCGGG 1425 COR-23134-4 Target 1381 TCTGGGTGCAAGGCTTGTTGCGG 1426 COR-23134-4 Target 1382 CCAGCTCCAGTATGACATCTGGG 1427 COR-23134-4 Target 1383 CCCAGCTCCAGTATGACATCTGG 1428 COR-23134-4 Target 1384 CTTGCACCCAGATGTCATACTGG 1429 COR-23134-4 Target 1385 CCAGATGTCATACTGGAGCTGGG 1430 COR-23134-4 Target 1386 GGGTAGGGAGATGTGCATCACGG 1431 COR-23134-4 Target 1387 GAATGTGGCTGCACTGGGTAGGG 1432 COR-23134-4 Target 1388 AGAATGTGGCTGCACTGGGTAGG 1433 COR-23134-4 Target 1389 TGCAAGAATGTGGCTGCACTGGG 1434 COR-23134-4 Target 1390 GTGCAAGAATGTGGCTGCACTGG 1435 COR-23134-4 Target 1391 GAAGGCCAAGTGCAAGAATGTGG 1436 COR-23134-4 Target 1392 TGCAGCCACATTCTTGCACTTGG 1437 COR-23134-4 Target 1393 CTCGGAGGTTCCGGCGTGGAAGG 1438 COR-23134-4 Target 1394 AAGTCTCGGAGGTTCCGGCGTGG 1439 COR-23134-4 Target 1395 GACGGAAGTCTCGGAGGTTCCGG 1440 COR-23134-4 Target 1396 TTGCACTTGGCCTTCCACGCCGG 1441 COR-23134-4 Target 1397 GATGCCGACGGAAGTCTCGGAGG 1442 COR-23134-4 Target 1398 GGAACCTCCGAGACTTCCGTCGG 1443 COR-23134-4 Target 1399 AGATTGAAGCCGTGGCAGAGGGG 1444 COR-23134-4 Target 1400 GAGATTGAAGCCGTGGCAGAGGG 1445 COR-23134-4 Target 1401 TGAGATTGAAGCCGTGGCAGAGG 1446 COR-23134-4 Target 1402 TGTGAATGAGATTGAAGCCGTGG 1447 COR-23134-4 Target 1403 ACATGCCCTCGGAGGCGGACTGG 1448 COR-23134-4 Target 1404 GCCAGACATGCCCTCGGAGGCGG 1449 COR-23134-4 Target 1405 TATGCCAGACATGCCCTCGGAGG 1450 COR-23134-4 Target 1406 ACCTATGCCAGACATGCCCTCGG 1451 COR-23134-4 Target 1407 AATACTCCAGTCCGCCTCCGAGG 1452 COR-23134-4 Target 1408 ATACTCCAGTCCGCCTCCGAGGG 1453 COR-23134-4 Target 1409 TCCGCCTCCGAGGGCATGTCTGG 1454 COR-23134-4 Target 1410 TCCGAGGGCATGTCTGGCATAGG 1455 COR-23134-4 Target 1411 GAGGGCATGTCTGGCATAGGTGG 1456 COR-23134-4 Target 1412 GGACCAGGTAAGTCAGATTGTGG 1457 COR-23134-4 Target 1413 CCTTGTGGATGCTCTGGACCAGG 1458 COR-23134-4 Target 1414 CATCCACAATCTGACTTACCTGG 1459 COR-23134-4 Target 1415 GGCTGCTATAAAGCACCTTGTGG 1460 COR-23134-4 Target 1416 CCTGGTCCAGAGCATCCACAAGG 1461 COR-23134-4 Target 1417 GGGTGGTGGAAATTCTAGAGGGG 1462 COR-23134-4 Target 1418 AGGGTGGTGGAAATTCTAGAGGG 1463 COR-23134-4 Target 1419 AAGGGTGGTGGAAATTCTAGAGG 1464 COR-23134-4 Target 1420 ACAAGCTCTGCGAAGGGTGGTGG 1465 COR-23134-4 Target 1421 GGCACAAGCTCTGCGAAGGGTGG 1466 COR-23134-4 Target 1422 AGCGGCACAAGCTCTGCGAAGGG 1467 COR-23134-4 Target 1423 AAGCGGCACAAGCTCTGCGAAGG 1468 COR-23134-4 Target 1424 TGTGAACATAGCCGGTCAAGCGG 1469 COR-23134-4 Target 1425 GCGCAAGCTGTGAACATAGCCGG 1470 COR-23134-4 Target 1426 CAGAGCTTGTGCCGCTTGACCGG 1471 COR-23134-4 Target 1427 GGCTGGTGCAACGGCTCCTGGGG 1472 COR-23134-4 Target 1428 CGGCTGGTGCAACGGCTCCTGGG 1473 COR-23134-4 Target 1429 ACGGCTGGTGCAACGGCTCCTGG 1474 COR-23134-4 Target 1430 ATTCGTCACGGCTGGTGCAACGG 1475 COR-23134-4 Target 1431 GCGCTCGCATTCGTCACGGCTGG 1476 COR-23134-4 Target 1432 GATTGCGCTCGCATTCGTCACGG 1477 COR-23134-4 Target 1433 CCAGAATGAGCAAGTTGCAAGGG 1478 COR-23134-4 Target 1434 TCCAGAATGAGCAAGTTGCAAGG 1479 COR-23134-4 Target 1435 CCCTTGCAACTTGCTCATTCTGG 1480 COR-23134-4 Target 1436 CCGACTGAGCTTGCAGATGGAGG 1481 COR-23134-4 Target 1437 GGACCGACTGAGCTTGCAGATGG 1482 COR-23134-4 Target 1438 CCTCCATCTGCAAGCTCAGTCGG 1483 COR-23134-4 Target 1439 GGACGACGCCATCGCTAAGATGG 1484 COR-23134-4 Target 1440 CAGTCGGTCCATCTTAGCGATGG 1485 COR-23134-4 Target 1441 TTCTCAAAGAAGGCAGGAGTTGG 1486 COR-23134-4 Target 1442 CAGCGGTCATTTCTCAAAGAAGG 1487 COR-23134-4 Target 1443 GATGGAGAGCTTTCATTCAGCGG 1488 COR-23134-4 Target 1444 TGCTGACAGAGAGGTGCAGATGG 1489 COR-23134-4 Target 1445 CAAGGCCTTTGCTGACAGAGAGG 1490 COR-23134-4 Target 1446 CTGCACCTCTCTGTCAGCAAAGG 1491 COR-23134-4 Target 1447 CTATTTGCTGCAGCAGAACAAGG 1492 COR-23134-4 Target 1448 GCTGCAGCAAATAGCTGCCAAGG 1493 COR-23134-4 Target 1449 GCAAATAGCTGCCAAGGATTTGG 1494 COR-23134-4 Target 1450 AGAACTACGACCAAGATTTCAGG 1495 COR-23134-4 Target 1451 ACTTGAGCTGCCTGAAATCTTGG 1496 COR-23134-4 Target 1452 CCTCATCAGACAAATGGCCCAGG 1497 COR-23134-4 Target 1453 GGTCGTAGTTCTGTGCTACCTGG 1498 COR-23134-4 Target 1454 GTCGTAGTTCTGTGCTACCTGGG 1499 COR-23134-4 Target 1455 TGGTAGCCTCATCAGACAAATGG 1500 COR-23134-4 Target 1456 CCTGGGCCATTTGTCTGATGAGG 1501 COR-23134-4 Target 1457 CAGCTGCAATACAGAATGTATGG 1502 COR-23134-4 Target 1458 TGCGGCACTACCGAGACAAACGG 1503 COR-23134-4 Target 1459 TTCAATGTGTCATGAAGATGGGG 1504 COR-23134-4 Target 1460 CATTCAATGTGTCATGAAGATGG 1505 COR-23134-4 Target 1461 AAGCGCAAGGCACTCGTTGTAGG 1506 COR-23134-4 Target 1462 ATCTCAGTTGCAAGGGACCGAGG 1507 COR-23134-4 Target 1463 AAGGCACTCGTTGTAGGCCTCGG 1508 COR-23134-4 Target 1464 AGAAGGAATCTCAGTTGCAAGGG 1509 COR-23134-4 Target 1465 CTTGCTCCGAGTTGTCCAGAAGG 1510 COR-23134-4 Target 1466 CTTGCAACTGAGATTCCTTCTGG 1511 COR-23134-4 Target 1467 GAGATTCCTTCTGGACAACTCGG 1512 COR-23134-4 Target 1468 AGAATTAGTGAGCGCTGTAGGGG 1513 COR-23134-4 Target 1469 CAGAATTAGTGAGCGCTGTAGGG 1514 COR-23134-4 Target 1470 CCAGAATTAGTGAGCGCTGTAGG 1515 COR-23134-4 Target 1471 CCTACAGCGCTCACTAATTCTGG 1516 COR-23134-4 Target 1472 GCGCTCACTAATTCTGGTGACGG 1517 COR-23134-4 Target 1473 CGCTCACTAATTCTGGTGACGGG 1518 COR-23134-4 Target 1474 GCACATTGCGGAGCTAGTGAAGG 1519 COR-23134-4 Target 1475 GCAAACCAGCGTGCACATTGCGG 1520 COR-23134-4 Target 1476 TAGCTCCGCAATGTGCACGCTGG 1521 COR-23134-4 Target 1477 ACCGATCCTAACATCTTGATGGG 1522 COR-23134-4 Target 1478 GACCGATCCTAACATCTTGATGG 1523 COR-23134-4 Target 1479 TGCATGCCCATCAAGATGTTAGG 1524 COR-23134-4 Target 1480 GCCCATCAAGATGTTAGGATCGG 1525 COR-23134-4 Target 1481 CACCGGTATTCCAGTGGAGGTGG 1526 COR-23134-4 Target 1482 GTACACCGGTATTCCAGTGGAGG 1527 COR-23134-4 Target 1483 AGAGTACACCGGTATTCCAGTGG 1528 COR-23134-4 Target 1484 GGATCGGTCACCACCTCCACTGG 1529 COR-23134-4 Target 1485 AATTTGCCCGTAGAGTACACCGG 1530 COR-23134-4 Target 1486 CACCACCTCCACTGGAATACCGG 1531 COR-23134-4 Target 1487 TGGAATACCGGTGTACTCTACGG 1532 COR-23134-4 Target 1488 GGAATACCGGTGTACTCTACGGG 1533 COR-23134-4 Target 1489 CTACGGGCAAATTCGTACTTTGG 1534 COR-23134-4 Target 1490 CGGGCAAATTCGTACTTTGGCGG 1535 COR-23134-4 Target 1491 TGTGAAACACTGAATGCCGTTGG 1536 COR-23134-4 Target 1492 GTGAAACACTGAATGCCGTTGGG 1537 COR-23134-4 Target 1493 TTTCGAGTGGTAGATCAATATGG 1538 COR-23134-4 Target 1494 CTGCTCGCACGAACAGAATTCGG 1539 COR-23134-4 Target 1495 TTCTGTTCGTGCGAGCAGTGTGG 1540 COR-23134-4 Target 1496 TACAACTGATCAGTCCGGTCGGG 1541 COR-23134-4 Target 1497 TTACAACTGATCAGTCCGGTCGG 1542 COR-23134-4 Target 1498 CAATTTACAACTGATCAGTCCGG 1543 COR-23134-4 Target 1499 CAGTGTGGTATCTACCCGACCGG 1544 COR-23134-4 Target 1500 ATCAGTTGTAAATTGAGTAATGG 1545 COR-23134-4 Target 1501 TACATTTAACCGCCCTGGGTGGG 1546 COR-23134-4 Target 1502 ATACATTTAACCGCCCTGGGTGG 1547 COR-23134-4 Target 1503 ACAATACATTTAACCGCCCTGGG 1548 COR-23134-4 Target 1504 CACAATACATTTAACCGCCCTGG 1549 COR-23134-4 Target 1505 GTAATGGAGCGAACCCACCCAGG 1550 COR-23134-4 Target 1506 TAATGGAGCGAACCCACCCAGGG 1551 COR-23134-4 Target 1507 TGGAGCGAACCCACCCAGGGCGG 1552 COR-23134-4 Target 1508 ACAGCGCTGTATTATAACCGCGG 1553 COR-23134-4 Target 1509 AAATGTATTGTGCATGGCCGCGG 1554 COR-23134-4 Target 1510 GTGGCCTTATCTGGTTGTTGAGG 1555 COR-23134-4 Target 1511 GTT...

Claims

1. A soybean plant comprising the genotype of the soybean event COR-23134-4, wherein said genotype comprises a nucleotide sequence as set forth in SEQ ID NO: 12 or SEQ ID NO: 15, or a nucleotide sequence having at least 90% sequence identity to SEQ ID NO: 12 or SEQ ID NO: 15.

2. The soybean plant of claim 1, wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 13 or SEQ ID NO: 16, or a nucleotide sequence having at least 90% sequence identity to SEQ ID NO: 13 or SEQ ID NO: 16.

3. The soybean plant of claim 1, wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 14 or SEQ ID NO: 17, or a nucleotide sequence having at least 90% sequence identity to SEQ ID NO: 14 or SEQ ID NO: 17.

4. A DNA construct comprising operably linked first, second, and third expression cassettes, wherein the first expression cassettes comprises:1) a zm-H2B Promoter;2) an ubiZMl Intron',3) an cry IB.

34. T, and4) an os-ubi Terminator,wherein the second expression cassette comprises:a) a gm-cab3 Promoter;b) a crylB.61.T, andc) an os-T28 Terminator',and wherein the third expression cassette comprises:i) a pv-ubi2 Promoter;ii) a pv-ubi2 Intron',iii) an ipdO83C; andiv) an os-Tl 7 Terminator.

5. A plant comprising the DNA construct of claim 4.

6. The plant of claim 5, wherein said plant is a soybean plant.

7. A plant comprising the sequence set forth in SEQ ID NO: 33, or a sequence having at least 95% sequence identity to SEQ ID NO: 33.

8. A soybean event COR-23134-4, wherein a representative sample of seed of said soybean event has been deposited with NCMA with NCMA Accession No.: 202305013.

9. Plant parts of the soybean event of claim 8.

10. Seed comprising soybean event COR-23134-4, wherein said seed comprises aDNA molecule chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein a representative sample of the soybean event COR-23134-4 seed of has been deposited with NCMA with NCMA Accession No.: 202305013.

11. A soybean plant, or part thereof, grown from the seed of claim 10.

12. A transgenic seed produced from the soybean plant of claim 8.

13. A transgenic soybean plant, or part thereof, grown from the seed of claim 12.

14. An isolated nucleic acid molecule comprising a nucleotide sequence chosen from SEQ ID NOs: 12-17, 33-37, and 41, and full length complements thereof.

15. An amplicon comprising the nucleic acid sequence chosen from SEQ ID NOs: 33-37, or 41, and full length complements thereof.

16. A biological sample derived from soybean event COR-23134-4 plant, tissue, or seed, wherein said sample comprises a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with NCMA with NCMA Accession No.: 202305013.

17. The biological sample of claim 16, wherein said biological sample comprises plant, plant tissue, or seed of transgenic soybean event COR-23134-4.

18. The biological sample of claim 17, wherein said biological sample is a DNA sample extracted from the transgenic soybean plant event COR-23134-4, and wherein said DNA sample comprises one or more of the nucleotide sequences chosen from SEQ ID NOs: 1217, 33-37, and 41, and the complement thereof.

19. The biological sample of claim 16, wherein said biological sample is chosen from soybean flour, soybean meal, and soybean oil manufactured in whole or in part to contain soybean by-products.

20. An extract derived from soybean event COR-23134-4 plant, tissue, or seed and comprising a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with NCMA with NCMA Accession No.: 202305013.

21. The extract of claim 20, wherein said nucleotide sequence is detectable in said extract using a nucleic acid amplification or nucleic acid hybridization method.

22. The extract of claim 21, wherein said extract comprises plant, plant tissue, or seed of transgenic soybean plant event COR-23134-4.

23. The extract of claim 22, wherein the extract is a composition chosen from soybean flour, soybean meal, and soybean oil manufactured in whole or in part to contain soybean byproducts, wherein said composition comprises a detectable amount of said nucleotide sequence.

24. A method of producing hybrid soybean seeds comprising:a. sexually crossing a first inbred soybean line comprising a nucleotide chosen from SEQ ID NOs: 12-17, 33-37, and 41 and a second inbred line having a different genotype;b. growing progeny from said crossing; andc. harvesting the hybrid seed produced thereby.

25. The method according to claim 24, wherein the first inbred soybean line is a female parent or a male parent.

26. A method for producing a soybean plant resistant to lepidopteran pests comprising:a. sexually crossing a first parent soybean plant with a second parent soybean plant, wherein said first or second parent soybean plant comprises event COR-23134-4 thereby producing a plurality of first-generation progeny plants;b. selfing the first-generation progeny plant, thereby producing a plurality of second-generation progeny plants; andc. selecting from the second-generation progeny plants that comprise the event COR-23134-4 and are resistant to a lepidopteran pest.

27. A method of producing hybrid soybean seeds comprising:a. sexually crossing a first inbred soybean line comprising the DNA construct of claim 4 with a second inbred line not comprising the DNA construct of claim 4; andb. harvesting the hybrid seed produced thereby.

28. The method of claim 26, further comprising the step of backcrossing a second-generation progeny plant that comprises soybean event COR-23134-4 to the parent plant that lacks the soybean event COR-23134-4 DNA, thereby producing a backcross progeny plant that is resistant to a lepidopteran pest.

29. A method for producing a soybean plant resistant to Lepidopteran pest, said method comprising:a. crossing a first parent soybean plant with a second parent soybean plant, wherein said first or second parent soybean plant comprises event COR-23134-4, thereby producing a plurality of first-generation progeny plants;b. selecting a first-generation progeny plant that comprises the event COR-23134-4;c. backcrossing the first-generation progeny plant of step (b) with a parent plant that lacks the soybean event COR-23134-4 DNA, thereby producing a plurality of backcross progeny plants; andd. selecting from the backcross progeny plants, a plant that comprises the event COR-23134-4;wherein the selected backcross progeny plant of step (d) comprises SEQ ID NO: 12-17, 3337, or 41.

30. The method according to claim 29, wherein the plants of the first parent soybean line are the female parents or male parents.

31. Hybrid seed produced by the method of claim 29.

32. A method of determining zygosity of a soybean plant comprising event COR-23134-4 in a biological sample comprising:a. contacting said sample with a first pair of DNA molecules and a second distinct pair of DNA molecules such that:i. when used in a nucleic acid amplification reaction comprising soybean event COR-23134-4 DNA, produces a first amplicon that is diagnostic for event COR-23134-4, andii. when used in a nucleic acid amplification reaction comprising soybean genomic DNA other than COR-23134-4 DNA, produces a second amplicon that is diagnostic for soybean genomic DNA other than COR-23134-4 DNA;b. performing a nucleic acid amplification reaction; andc. detecting the amplicons so produced, wherein detection of the presence of both amplicons indicates that said sample is heterozygous for soybean event COR-23134-4 DNA, wherein detection of only the first amplicon indicates that said sample is homozygous for soybean event COR-23134-4 DNA.

33. The method of claim 32, wherein the first pair of DNA molecules comprises primer pair SEQIDNOs: 18 and 19.

34. The method of claim 32, wherein the first and second pair of DNA molecules comprise a detectable label.

35. The method of claim 34, wherein the detectable label is a fluorescent label.

36. The method of claim 34, wherein the detectable label is covalently associated with one or more of the primer molecules.

37. A method of detecting the presence of a nucleic acid molecule that is unique to event COR-23134-4 in a sample comprising soybean nucleic acids, the method comprising:a. contacting the sample with a pair of primers that, when used in a nucleic-acid amplification reaction with genomic DNA from event COR-23134-4 produces an amplicon that is diagnostic for event COR-23134-4;b. performing a nucleic acid amplification reaction, thereby producing the amplicon that is diagnostic for event COR-23134-4; andc. detecting the amplicon that is diagnostic for event COR-23134-4.

38. The method of claim 37 wherein the nucleic acid molecule that is diagnostic for event COR-23134-4 is an amplicon produced by the nucleic acid amplification chain reaction.

39. The method of claim 37, wherein the method further comprises contacting the sample with a probe.

40. The method of claim 39, wherein the probe comprises a detectable label.

41. The method of claim 40, wherein the detectable label is covalently associated with the probe.

42. A plurality of polynucleotide primers comprising one or more polynucleotides which target event COR-23134-4 DNA template in a sample to produce an amplicon diagnostic for event COR-23134-4 as a result of a polymerase chain reaction method.

43. The plurality of polynucleotide primers according to claim 42, whereina. a first polynucleotide primer comprises a nucleotide sequence as set forth in SEQ ID NO: 18, and the complements thereof; andb. a second polynucleotide primer comprises a nucleotide sequence as set forth in SEQ ID NO: 19, and the complements thereof.

44. The primers of claim 43, wherein said first primer and said second primer are at least 19 nucleotides.

45. A method of detecting the presence of DNA corresponding to event COR-23134-4 in a sample, the method comprising:a. contacting the sample comprising soybean DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from soybean event COR-23134-4 and does not hybridize under said stringent hybridization conditions with a non-COR-23134-4 soybean plant DNA;b. subjecting the sample and probe to stringent hybridization conditions; andc. detecting hybridization of the probe to the DNA;wherein detection of hybridization indicates the presence of event COR-23134-4.

46. A kit for detecting nucleic acids that are unique to event COR-23134-4 comprising at least one nucleic acid molecule of sufficient length of contiguous polynucleotides to function as a primer or probe in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of nucleic acid sequences unique to event COR-23134-4 in the sample.

47. The kit according to claim 46, wherein the nucleic acid molecule comprises a nucleotide sequence from SEQ ID NO: 12-41.

48. The kit according to claim 46, wherein the nucleic acid molecule is a primer chosen from SEQ ID NOs: 12-41, and the complements thereof.

49. The soybean plant of claim 3, wherein the genotype comprises a nucleotide sequence having 1, 2, 3, 4, or 5 nucleotide changes in one of SEQ ID NO: 14 or SEQ ID NO: 17.

50. The soybean plant of claim 1, further comprising the nucleotide sequence set forth in SEQ ID NO: 3, or a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO: 3.

51. A method of modifying the COR-23134-4 soybean event, wherein a representative sample of seed of said soybean event was deposited with the NCMA with NCMA Accession No.: 202305013, comprising applying genome engineering technology to a DNA sequence of said COR-23134-4 soybean event to modify the DNA of said soybean event.

52. The method of claim 51, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having at least 90% sequence identity to SEQ ID NO: 3.

53. The method of claim 51, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having all or a portion of SEQ ID NO: 12 or SEQ ID NO: 15 duplicated in said modified DNA sequence.

54. The method of claim 51, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence comprising an excision from SEQ ID NO: 3.

55. The method of claim 54, wherein said excision comprises an excision from one or more regulatory elements of SEQ ID NO: 3 that does not substantially affect the activity of said one or more regulatory elements.

56. The method of claim 54, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having all or a portion of SEQ ID NO: 12 or SEQ ID NO: 15 excised from said modified DNA sequence.

57. The method of claim 54, comprising modifying the DNA of said COR-23134-4 soybean event to produce a modified DNA sequence having at least 30% of SEQ ID NO: 3 excised from said modified DNA sequence.

58. The method of claim 57, wherein at least 80% of SEQ ID NO: 3 is excised from said modified DNA sequence.

59. The method of claim 57, wherein all of SEQ ID NO: 3 is excised from said modified DNA sequence.

60. A method of generating guide polynucleotides for use with a COR-23134-4 soybean event genome editing system comprising designing one or more guide polynucleotides that recognize at least a portion of SEQ ID NO: 3 and synthesizing said guide polynucleotides.

61. A method of modifying the DNA of the COR-23134-4 event having accession No.: 202305013 comprising introducing said one or more guide polynucleotides of claim 60 aspart of a genome engineering composition to a DNA of the COR-23134-4 event to modify the DNA of the COR-23134-4 event.

62. A COR-23134-4 soybean event genome editing system comprising a CAS polypeptide, one or more guide polynucleotides, and COR-23134-4 soybean event donor DNA.

63. A method of modifying at least one expression cassette of the COR-23134-4 event as deposited with the NCMA having accession number NCMA ACCESSION NO.: 202305013, wherein the method comprises using genome editing technologies to modify at least one expression cassette, wherein the resulting soybean plant derived from the COR-23134-4 event comprises at least one modified cassette.

64. The method of claim 63, wherein the method comprises altering expression of at least one of the polynucleotide coding sequences selected from the group consisting of: cry IB. 34.1, crylB.61.1, and ipdO83Cb.

65. A method of producing a commodity plant product comprising processing grain produced from a soybean event COR-23134-4 plant comprising a nucleotide sequence which is or is complementary to a sequence chosen from SEQ ID NO: 12 and SEQ ID NO: 15, wherein a representative sample of said soybean event COR-23134-4 seed has been deposited with NCMA with NCMA Accession No.: 202305013, wherein said grain is processed into a commodity plant product chosen from soybean flour, soybean meal, and soybean oil manufactured in whole or in part to contain soybean by-products, wherein said composition / commodity plant product comprises a detectable amount of said nucleotide sequence.

66. A method of controlling Lepidopteran insects, comprising exposing the Lepidopteran insects to insect resistant soybean plants of event COR-23134-4.

67. The method of claim 66, wherein the Lepidopteran insect is Fall Armyworm (Spodoptera frugiperda).

68. The method of claim 66, wherein the Lepidopteran insect is Soybean Looper (Chrysodeixis includens).

69. The method of claim 66, wherein the Lepidopteran insect is Velvetbean Caterpillar (Anticar sia gemmatalis).IQ. The method of claim 66, wherein damage from the Lepidopteran insect is controlled for soybean from event COR-23134-4.

Images