A sealed high-throughput and efficient fully automatic biosynthesis device

Abstract

The present invention discloses a sealed high-throughput, efficient, fully automatic biosynthesis device, which includes a moving plate that performs horizontal linear motion, a plurality of synthesis chamber units that are fixed below the moving plate and form an upper opening on the moving plate, a sealing plate assembly that is located above the moving plate and forms a sealed space with the moving plate through up and down motion, a sample loading unit fixed on the sealing plate assembly, and a plurality of pressurized sealing units that are fixed on the sealing plate assembly and seal the upper opening, wherein the moving plate is driven by a driving member to move between the sample loading unit position and the pressurized sealing unit position. The present invention realizes the automatic addition of sample amount and the automatic transfer of primers to the sealed reaction chamber during the synthesis reaction of large-scale ultra-trace biological samples, and performs automatic pressurization and pressure maintenance, and precisely controls the pressure and synthesis speed required for synthesis, thereby greatly improving the efficiency of biosynthesis.

Description

[Technical field]

[0001] The invention belongs to the technical field of biosynthesis devices, and in particular relates to a sealed high-throughput, high-efficiency, fully-automatic biosynthesis device. [Background technology]

[0002] At present, DNA synthesis column often adopts single column tube or 96-well filter plate. The existing single column tube has many specifications and large volume, which is not conducive to batch, large-scale and miniaturized operation. For trace, ultra-micro and large-scale DNA synthesis, single column tube is difficult to achieve; the existing 96-well filter plate mainly has two specifications of 1.5 and 2ml, which can complete the synthesis of 96 DNA samples at the same time. It is much faster than single tube for the synthesis of batch DNA, but it still cannot meet the batch and large-scale synthesis of trace and ultra-micro DNA in terms of the sample volume processed. Therefore, 384-well plates have been developed on the market, which can complete the synthesis of 384 DNA samples at the same time. But it is a very huge task for the sample addition of 384-well plates. In the prior art, there is no sample addition device that can cooperate with the high-density sample addition of 384-well plates, resulting in low sample addition efficiency and a long synthesis time. During the synthesis process, the synthesis plate loaded with reagents needs to be in a sealed environment according to the requirements of the synthesis conditions, and also needs to be filled with inert gas for protection. However, in the prior art, the sealing of the sealed chamber for synthesis is poor, and when adding samples and reagents, all operations are done manually, which has a very large workload and extremely low efficiency. The sample volume control accuracy is poor, and the synthesis conditions are unstable, resulting in poor synthesis efficiency.

[0003] Therefore, it is necessary to provide a new sealed high-throughput and efficient fully automatic biosynthesis device to solve the above problems. [Summary of the invention]

[0004] The main purpose of the present invention is to provide a sealed high-throughput and efficient fully automatic biosynthesis device, which can realize the automatic addition of sample amounts and the automatic transfer of primers to a sealed reaction chamber during the synthesis reaction of large-scale ultra-trace biological samples, and automatically pressurize and maintain the pressure, precisely control the pressure and synthesis speed required for synthesis, and greatly improve the efficiency of biosynthesis.

[0005] The present invention achieves the above-mentioned purpose through the following technical scheme: a sealed high-throughput, high-efficiency, fully automatic biosynthesis device, which includes a moving plate that performs horizontal linear motion, a plurality of synthesis chamber units fixed below the moving plate and forming an upper opening on the moving plate, a sealing plate assembly located above the moving plate and forming a sealed space with the moving plate through up and down motion, a sample loading unit fixed on the sealing plate assembly, and a plurality of pressurized sealing units fixed on the sealing plate assembly and sealing the upper opening, wherein the moving plate is driven by a driving member to move between the sample loading unit position and the pressurized sealing unit position.

[0006] Further, the synthesis chamber unit includes a drainage collection box, and a synthesis plate located on the top of the drainage collection box and connected to the inner cavity of the drainage collection box;

[0007] The drainage collection box is located below the upper opening and the top of the surrounding baffles is sealed and attached to the lower surface of the moving plate, so that a sealed space is formed below the synthetic board.

[0008] Furthermore, a plurality of synthetic holes are arranged on the synthetic plate, the upper portion of the synthetic holes is a square opening, and the synthetic holes are divided into a fourth hole segment and a fifth hole segment from top to bottom, the fourth hole segment is an inverted cone hole or a square hole, and the fifth hole segment is a cylindrical hole or an inverted cone hole.

[0009] Furthermore, the sealing plate assembly includes a sealing support plate covering the movable plate, a first sealing ring fixed on the lower surface of the sealing support plate, and a driving cylinder driving the sealing support plate to move up and down.

[0010] Furthermore, the sample adding unit includes a support plate, a plurality of distribution tube mounting seats arranged on the support plate, and a plurality of distribution tubes arranged on the distribution tube mounting seats, and the other end of the distribution tube is connected to the automatic liquid injection device.

[0011] Furthermore, the distribution pipe mounting seat is provided with a plurality of first mounting holes penetrating the upper and lower surfaces, the first mounting holes are divided into a first hole section, a second hole section and a third hole section from top to bottom, the second hole section is a threaded hole, and the distribution pipe is provided with an external thread structure cooperating with the threaded hole near the lower area.

[0012] Furthermore, a plug is inserted into the lower end of the distribution pipe, and the third hole section is a tapered hole that is larger at the top and smaller at the bottom. The third hole section uses the tapered hole structure to squeeze the lower section of the distribution pipe to seal it with the plug, and holds the plug to achieve a fixed sealed connection between the two.

[0013] Furthermore, the pressurized sealing unit includes a frame plate bracket fixed on the sealing plate assembly, a driving cylinder fixed on the four corner areas of the frame plate bracket, a sealing pressurization module driven by the driving cylinder to move up and down, and a second sealing ring that seals the contact area between the lower surface of the sealing pressurization module and the peripheral surface of the upper opening of the synthetic chamber unit.

[0014] Furthermore, the sealing and pressurizing module includes an upper pad and a lower pad in a rectangular frame structure, an observation window plate arranged between the upper pad and the lower pad, a third sealing ring for sealing the contact portion between the upper pad and the observation window plate, and a fourth sealing ring for sealing the contact portion between the lower pad and the observation window plate.

[0015] Furthermore, the lower pad and the observation window plate jointly enclose a pressurized cabin with an opening facing downward, and a pressurized air duct connected to the pressurized cabin is formed inside the upper pad and the lower pad, and the pressurized air duct is connected to a pressurizing device pipeline.

[0016] Compared with the prior art, the beneficial effects of the sealed high-throughput and efficient fully automatic biosynthesis device of the present invention are: it realizes automatic liquid addition and automatic pressurized sealed synthesis for large-scale and ultra-trace nucleic acid synthesis, greatly improving the synthesis efficiency and synthesis quality, and can flexibly control the pressure of the reaction sealing cabin according to the reaction condition requirements, and control the time for the liquid to flow through the synthesis carrier; by arranging the sample addition station and the synthesis reaction station separately, the problem of liquid dripping at the bottom of the sample addition nozzle is effectively avoided, and the sealing of the biosynthesis reaction environment is greatly improved by arranging the sealing plate assembly and the pressurized sealing unit, thereby ensuring the effective progress of the synthesis reaction; by arranging the distribution pipe external thread and the distribution pipe mounting seat structure, the distribution pipe setting density is greatly improved; the problem of large distribution space of the original pipe fittings and pipeline shapes is solved; the general pipe fittings cannot achieve a 4.5*4.5mm matrix distribution; and the manufacturing cost is expensive; the structural parts of this scheme are improved. The manufacturability of the system is improved. The distributed pressure layout solves the pressure load at the distribution pipeline position and allows a simple mechanism to achieve sealing. The synthesis efficiency is greatly improved by setting a high-density distribution pipe. The distribution pipe has a high density. It can effectively reduce the displacement distance of the moving plate, reduce the single operation time, and improve the synthesis efficiency. At the same time, it effectively increases the number of distribution paths, and can provide more types of reagents at the same distance. Through algorithm optimization, high-throughput synthesis can be achieved. By setting a high-density distribution pipe, the equipment size is greatly reduced, so that the size of the pipe parts can be controlled within the range of general processing capabilities, greatly reducing the processing cost. At the same time, effective space control can provide sufficient design possibilities for other components, and finally optimize a more stable structure. By setting the pressure distribution in different areas, synthesis within the positive pressure range can be achieved, which greatly reduces the pressure on public works, and does not require negative pressure and a large amount of waste gas and waste liquid treatment equipment.

Brief Description of the Drawings

[0017] Figure 1 It is a structural schematic diagram of an embodiment of the present invention;

[0018] Figure 2 A schematic diagram of the structure of a bracket in an embodiment of the present invention;

[0019] Figure 3 It is a schematic diagram of the structure of the moving plate and the synthesis cavity unit in an embodiment of the present invention;

[0020] Figure 4 It is a structural schematic diagram of a synthesis cavity unit in an embodiment of the present invention;

[0021] Figure 5 It is a partial cross-sectional structural schematic diagram of a synthetic board in an embodiment of the present invention;

[0022] Figure 6 Schematic diagram of the assembly structure of the sealing plate assembly, the sample adding unit and the pressurizing sealing unit in an embodiment of the present invention;

[0023] Figure 7 It is a schematic diagram of the explosion structure of the sealing plate assembly, the sample adding unit and the pressurizing sealing unit in the embodiment of the present invention;

[0024] Figure 8 This is a schematic diagram of the structure of the sample adding unit in an embodiment of the present invention;

[0025] Fig. 9 It is a partial structural schematic diagram of a sample adding component in an embodiment of the present invention;

[0026] Fig.10 It is a partial enlarged structural schematic diagram of the distribution pipe mounting seat in an embodiment of the present invention;

[0027] Fig.11 It is a partial cross-sectional structural schematic diagram of the distribution pipe and the insert pipe after being assembled on the distribution pipe mounting seat in the embodiment of the present invention;

[0028] Fig.12 This is a schematic structural diagram of a pressurized sealing unit in an embodiment of the present invention;

[0029] Fig.13 is a schematic cross-sectional structural diagram of a pressurized sealing unit in an embodiment of the present invention;

[0030] The numbers in the figure represent:

[0031] 100 sealed high-throughput and efficient fully automatic biosynthesis device;

[0032] 1 moving plate, 11 upper opening; 2 synthesis chamber unit, 21 drainage collection box, 22 synthesis plate, 221 synthesis hole, 222 fourth hole section, 223 fifth hole section, 224 synthesis carrier, 23 support frame plate, 24 door baffle, 25 observation window, 26 waste liquid collection box;

[0033] 3 sealing plate assembly, 31 sealing support plate, 32 first sealing ring, 33 driving cylinder;

[0034] 4 sample adding unit, 41 support plate, 411 strip hole, 42 distribution tube mounting seat, 421 first mounting hole, 4211 first hole section, 4212 second hole section, 4213 third hole section, 422 strip support portion, 423 limiting guide portion, 424 second mounting hole, 43 distribution tube, 431 external thread structure, 44 inserting tube;

[0035] 5 pressurized sealing unit, 51 frame bracket, 52 driving cylinder, 53 sealing pressurized module, 531 upper pad, 532 lower pad, 533 observation window plate, 534 third sealing ring, 535 fourth sealing ring, 536 bolt group, 537 pressurized airway, 538 pressurized cabin, 54 second sealing ring; 6 driving part; 7 support, 71 supporting roller group, 72 limit retaining ring. [Specific implementation method]

[0036] Example:

[0037] Please refer to Figure 1-Figure 13 The present embodiment is a sealed high-throughput, high-efficiency, fully-automatic biosynthesis device 100, which includes a moving plate 1 that performs horizontal linear motion, a plurality of synthesis chamber units 2 that are fixed below the moving plate 1 and form an upper opening 11 on the moving plate 1, a sealing plate assembly 3 that is located above the moving plate 1 and forms a sealed space with the moving plate 1 through up and down motion, a sample loading unit 4 that is fixed on the sealing plate assembly 3, and a plurality of pressurizing sealing units 5 that are fixed on the sealing plate assembly 3 and seal the upper opening 11. The moving plate 1 is driven by a driving member 6 to move between the position of the sample loading unit 4 and the position of the independent pressurizing sealing unit 5.

[0038] This embodiment also includes a support 7, on which a support roller set 71 is provided, and the moving plate 1 is mounted on the support roller set 71 for horizontal movement. Limit stop rings 72 are also provided at both ends of the support 7 to ensure that there is no obstacle in the moving range of the moving plate 1, and on the other hand, to improve safety.

[0039] The synthesis chamber unit 2 includes a drainage collection box 21, and a synthesis board 22 located at the top of the drainage collection box 21 and connected to the inner cavity of the drainage collection box 21. The synthesis board 22 is fixed to the upper opening 11 through a support frame plate 23. The drainage collection box 21 is located below the upper opening 11 and the top of the surrounding baffles is sealed against the lower surface of the movable plate 1, so that a sealed space is formed below the synthesis board 22. An openable door baffle 24 is provided on one side of the drainage collection box 21, and an observation window 25 is provided on the door baffle 24 to facilitate observation of the situation in the drainage collection box 21.

[0040] In this embodiment, two groups of synthesis chamber units 2 are provided, and a waste liquid collection box 26 is provided between two adjacent synthesis chamber units 2. The functions of the waste liquid collection box 26 are:

[0041] 1) Provide a blank group setting space during the debugging process; no need for personnel to receive liquid separately;

[0042] 2) Provide drainage space during the synthesis process, before cleaning and initial discharge; pre-drain the liquid in the dead zone; prevent bubbles and conditions that are not within the control range;

[0043] 3) It is convenient to observe the liquid discharge during the synthesis process;

[0044] 4) Optimize the process and program structure to provide air defense positions for the process.

[0045] The synthesis plate 22 is a 384-well plate or a 96-well plate or other number of well plates. A plurality of synthesis holes 221 are arranged on the synthesis plate 22. The upper part of the synthesis hole 221 is a square opening. The synthesis hole 221 is divided into a fourth hole segment 222 and a fifth hole segment 223 from top to bottom. The fourth hole segment 222 is an inverted cone hole or a square hole, and the fifth hole segment 223 is a cylindrical hole or an inverted cone hole. When performing DNA nucleic acid synthesis, the synthesis carrier 224 is set in the synthesis hole 221. The synthesis carrier 224 is a cylindrical solid phase synthesis carrier, and the tip is pushed down along the square opening. The bottom end of the synthesis carrier 224 is fixed in the fifth hole segment 223 and the top end extends into the fourth hole segment 222.

[0046] The sealing plate assembly 3 includes a sealing support plate 31 covering the upper portion of the moving plate 1, a first sealing ring 32 fixed to the lower surface of the sealing support plate 31, and a driving cylinder 33 for driving the sealing support plate 31 to move up and down. As the sealing support plate 31 descends, the first sealing ring 32 abuts against the surface of the moving plate 1 to form a sealed space, thereby sealing the upper space of the moving plate 1 covering the sample loading unit 4 and the pressurized sealing unit 5, and cooperating with the drainage collection box 21 under the moving plate 1 so that the synthesis plate 22 is located in a completely sealed environment, thereby improving the environment of biosynthesis and greatly shortening the time that reagents and samples are exposed to the air.

[0047] The sample adding unit 4 includes a support plate 41, a plurality of distribution tube mounting seats 42 arranged on the support plate 41, and a plurality of distribution tubes 43 arranged on the distribution tube mounting seats 42. The other end of the distribution tube 43 is connected to an automatic liquid injection device (not shown in the figure). The distribution tubes 43 are arranged in a one-to-one correspondence with the synthetic holes 221 in the synthetic plate 22. The distribution tube mounting seat 42 is provided with a plurality of first mounting holes 421 penetrating the upper and lower surfaces. The first mounting holes 421 are divided into a first hole section 4211, a second hole section 4212 and a third hole section 4213 from top to bottom. The second hole section 4212 is a threaded hole, and the third hole section 4213 is a tapered hole with a larger upper part and a smaller lower part. The distribution tube 43 is provided with an external thread structure 431 matching the threaded hole near the lower area. The inner diameter of the first hole section 4211 is larger than the diameter of the second hole section 4212. The automatic liquid injection device can adopt the existing structure on the market.

[0048] The distribution pipe mounting seat 42 is in a strip-shaped structure and is arranged one by one or arranged at intervals on the support plate 41. The distribution pipe mounting seat 42 includes a strip-shaped support portion 422 and a limiting guide portion 423 located on the lower surface of the strip-shaped support portion 422. The area surrounded by the outer contour of the limiting guide portion 423 is located within the range of the area surrounded by the outer contour of the strip-shaped support portion 422. The support plate 41 is provided with a plurality of strip-shaped holes 411 for the limiting guide portion 423 to pass through.

[0049] The lower end of the distribution tube 43 is also plugged with a plug 44, the upper end of the plug 44 is plugged into the inner hole of the distribution tube 43 and the bottom end extends out of the lower surface of the distribution tube mounting seat 42. The third hole section 4213 uses its conical hole structure to squeeze the lower section area of ​​the distribution tube 43 to seal it with the plug 44, and holds the plug 44 to achieve a fixed sealed connection between the two, preventing the plug 44 from slipping and moving downward, and ensuring that the extended length of the plug 44 remains unchanged. When the inner diameter of the distribution tube 43 is small, the sample addition operation can also be performed without inserting the plug 44.

[0050] The pressurized sealing unit 5 includes a frame support 51 fixed on the sealing support plate 31, a driving cylinder 52 fixed on the four corners of the frame support 51, a sealing pressurized module 53 driven by the driving cylinder 52 to move up and down, and a second sealing ring 54 for sealing the contact area between the lower surface of the sealing pressurized module 53 and the peripheral surface of the upper opening of the synthesis chamber unit 2. The sealing pressurized module 53 includes an upper pad 531 and a lower pad 532 in a rectangular frame structure, an observation window plate 533 arranged between the upper pad 531 and the lower pad 532, a third sealing ring 534 for sealing the contact portion between the upper pad 531 and the observation window plate 533, a fourth sealing ring 535 for sealing the contact portion between the lower pad 532 and the observation window plate 533, and a plurality of bolt groups 536 for locking the upper pad 531 and the lower pad 532. The lower pad 532 and the observation window plate 533 together form a pressurized cabin 538 with an opening facing downward, and a pressurized airway 537 communicating with the pressurized cabin 538 is formed inside the upper pad 531 and the lower pad 532, and the pressurized airway 537 is communicated with a pressurizing device (not shown) by pipeline. The pressurizing device can adopt an existing structure on the market.

[0051] When the sealing and pressurizing module 53 is at the upper limit height position, its upper surface is in close contact with the inner wall surface of the frame plate support 51. In other embodiments, a fifth sealing ring (not shown) can be provided to seal the contact area between the sealing and pressurizing module 53 and the frame plate support 51.

[0052] In this embodiment, two groups of pressurizing and sealing units 5 are provided, corresponding one to one to the two groups of synthesis chamber units 2 .

[0053] The working principle of the sealed high-throughput, high-efficiency, fully automatic biosynthesis device 100 of this embodiment is as follows: in the initial state, the sealing support plate 31 is located at the upper limit height position; the synthesis chamber unit 2 is driven by the driving member 6 to move to the bottom of the sample adding unit 4; the sealing support plate 31 descends to press the surface of the moving plate 1, and the upper space of the moving plate 1 is sealed by the first sealing ring 32; the distribution tube 43 in the sample adding unit 4 is connected through an external automatic liquid injection device, and the reagents or samples required for the reaction are added, and the reagents or samples are dripped into the synthesis plate 22 through the end of the distribution tube 43, and the sample adding unit 4 can complete the addition of one or two groups of 384 samples at one time; After the sample is completed, the sealing support plate 31 rises, and the driving member 6 drives the synthesis chamber unit 2 to move to the bottom of the pressurized sealing unit 5, and the sealing support plate 31 descends. At the same time, the sealing pressurization module 53 descends to cover the upper opening of the synthesis plate 22, and the inert gas or other protective gas is blown into the pressurized air duct 537 through the pressurized air chamber 538 through the pressurized airway 537, so that the synthesis plate 22 is placed in the sealed space with inert gas protection and pressurized environment. The synthesis situation can be observed through the observation window plate 533, and the synthesized reagent is discharged from the end of the synthesis carrier 224 at the bottom of the synthesis plate 22 into the drainage collection box 21, and it can be taken out by opening the door baffle 24.

[0054] The beneficial effects of the sealed high-throughput, high-efficiency, fully automatic biosynthesis device 100 of this embodiment are: it realizes automatic liquid addition and automatic pressurized sealed synthesis for large-scale and ultra-trace nucleic acid synthesis, greatly improving the synthesis efficiency and synthesis quality; the pressure of the reaction sealed cabin can be flexibly controlled according to the reaction condition requirements, and the time for the liquid to flow through the synthesis carrier can be controlled; by setting the sample addition station and the synthesis reaction station separately, the problem of liquid dripping at the bottom of the sample addition nozzle is effectively avoided; by setting the sealing plate assembly and the pressurized sealing unit, the sealing of the biosynthesis reaction environment is greatly improved, and the effective progress of the synthesis reaction is guaranteed; by setting the external thread of the distribution pipe and the distribution plate structure, the distribution pipe setting is greatly improved. density; solved the problem of large distribution space of original pipe fittings and pipeline shapes; through the dual-cavity setting, multiple mode switching can be realized; greatly improved the synthesis amount or synthesis type; through the synchronous synthesis of two cavities; or separate synthesis; improved the synthesis efficiency; through the setting of the sealing device between the moving plate and the distribution plate; greatly reduced the sealing responsibility, and realized the effective sealing in the moving state; through the setting of the cam follower, the manufacturability of the equipment is greatly improved, and a flexible moving space is provided for the long-stroke moving plate; at the same time, the cost is reduced; through the setting of the cavity window, the controllability of the synthesis is greatly improved; the synthesis process can be timely monitored through the four windows set to ensure the synthesis quality.

[0055] The above are only some embodiments of the present invention. For those skilled in the art, several modifications and improvements can be made without departing from the creative concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A sealed high-throughput, high-efficiency, fully automatic biosynthesis device, characterized in that: It comprises a moving plate that performs horizontal linear motion, a plurality of synthesis chamber units that are fixed below the moving plate and form an upper opening on the moving plate, a sealing plate assembly that is located above the moving plate and forms a sealed space with the moving plate through up and down motion, a sample loading unit that is fixed on the sealing plate assembly, and a plurality of pressurizing sealing units that are fixed on the sealing plate assembly and seal the upper opening, wherein the moving plate is driven by a driving member to move between the sample loading unit position and the pressurizing sealing unit position; The sample loading unit comprises a support plate, a plurality of distribution tube mounting seats arranged on the support plate, and a plurality of distribution tubes arranged on the distribution tube mounting seats, wherein the distribution tube mounting seats are in a strip-shaped structure and are arranged one by one or arranged at intervals on the support plate; the distribution tube mounting seats comprise a strip-shaped support portion, and a position limiting guide portion located on the lower surface of the strip-shaped support portion; the area surrounded by the outer contour of the position limiting guide portion is located within the range of the area surrounded by the outer contour of the strip-shaped support portion; and the support plate is provided with a plurality of strip-shaped holes for the position limiting guide portion to pass through; The other end of the distribution pipe is connected to the automatic liquid injection device; the distribution pipe mounting seat is provided with a plurality of first mounting holes penetrating the upper and lower surfaces, the first mounting holes are divided into a first hole section, a second hole section and a third hole section from top to bottom, the second hole section is a threaded hole, and the distribution pipe is provided with an external thread structure matching the threaded hole near the lower area; a plug is inserted into the lower end of the distribution pipe, and the third hole section is a tapered hole with a larger upper part and a smaller lower part, and the third hole section uses the tapered hole structure to squeeze the lower section of the distribution pipe to seal it with the plug, and holds the plug to achieve a fixed and sealed connection between the two.

2. The sealed high-throughput, high-efficiency, fully-automatic biosynthesis device according to claim 1, characterized in that: The synthesis chamber unit comprises a drainage collection box, and a synthesis plate located on the top of the drainage collection box and connected to the inner cavity of the drainage collection box; The drainage collection box is located below the upper opening and the top of the surrounding baffles is sealed and attached to the lower surface of the moving plate, so that a sealed space is formed below the synthetic board.

3. The sealed high-throughput, high-efficiency, fully-automatic biosynthesis device according to claim 2, characterized in that: A plurality of synthetic holes are arranged on the synthetic plate, the upper portion of the synthetic holes is a square opening, and the synthetic holes are divided into a fourth hole segment and a fifth hole segment from top to bottom, the fourth hole segment is an inverted cone hole or a square hole, and the fifth hole segment is a cylindrical hole or an inverted cone hole.

4. The sealed high-throughput, high-efficiency, fully-automatic biosynthesis device according to claim 1, characterized in that: The sealing plate assembly includes a sealing support plate covering the upper portion of the movable plate, a first sealing ring fixed to the lower surface of the sealing support plate, and a driving cylinder driving the sealing support plate to move up and down.

5. The sealed high-throughput, high-efficiency, fully-automatic biosynthesis device according to claim 1, characterized in that: The pressurized sealing unit includes a frame plate bracket fixed on the sealing plate assembly, a driving cylinder fixed on the four corner areas of the frame plate bracket, a sealing pressurization module driven by the driving cylinder to move up and down, and a second sealing ring that seals the contact area between the lower surface of the sealing pressurization module and the peripheral surface of the upper opening of the synthetic chamber unit.

6. The sealed high-throughput, high-efficiency, fully-automatic biosynthesis device according to claim 5, characterized in that: The sealing and pressurizing module includes an upper pad and a lower pad in a rectangular frame structure, an observation window plate arranged between the upper pad and the lower pad, a third sealing ring for sealing the contact portion between the upper pad and the observation window plate, and a fourth sealing ring for sealing the contact portion between the lower pad and the observation window plate.

7. The sealed high-throughput, high-efficiency, fully-automatic biosynthesis device according to claim 6, characterized in that: The lower pad and the observation window plate jointly enclose a pressurized cabin with an opening facing downward, and a pressurized air duct communicating with the pressurized cabin is formed inside the upper pad and the lower pad, and the pressurized air duct is communicated with a pressurizing device pipeline.

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