Plant moisturizing water and method for preparing the same

Abstract

This invention relates to the field of cosmetic technology, and in particular to a plant-based moisturizing water and its preparation method. The plant-based moisturizing water comprises the following components by weight: 80-96 parts solvent, 1-10 parts humectant, 0.01-0.3 parts thickener, and 0.01-0.1 parts chelating agent; the humectant includes Dendrobium officinale stem extract and Hydrangea macrophylla extract in a mass ratio of (1-5):(1-5). The moisturizing water of this invention, through the rational combination of its components, can effectively increase the skin's moisture content, provide long-lasting hydration, and has a pleasant, non-sticky feel.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a plant-based moisturizing water and its preparation method. Background Technology

[0002] Skin is a living tissue that relies on moisture to achieve optimal function and performance. When the moisture content of the stratum corneum drops to 10%, it leads to dryness, dullness, decreased elasticity, and increased wrinkles—signs of skin aging. It also weakens the skin barrier function, making it easier for harmful external substances to penetrate, potentially causing serious skin diseases. Therefore, moisturizing plays an indispensable role in cosmetic skincare products, and hydrating toners, as a foundational product in the hydrating and moisturizing skincare line, have a promising consumer market prospect.

[0003] The main ingredient in moisturizing toners is a humectant. Common traditional skincare humectants include glycerin, sorbitol, 1,2-propanediol, 1,3-butanediol, sodium pyrrolidone carboxylate, and polyethylene glycol. Newer skincare humectants include betaine, chitosan, panthenol, collagen, sodium hyaluronate, tea polyphenols, ceramides, and silk peptides. In actual production, using a single humectant often fails to achieve the desired moisturizing effect; instead, multiple humectants are used in combination, increasing the complexity of the formula. Most plant-based moisturizing toners lack a comprehensive understanding of the skin's moisturizing system and cannot effectively promote its own moisturizing mechanisms. Furthermore, most moisturizing toners are too thin, lacking a noticeable smooth feel on the skin, absorbing slowly, and while they offer some moisturizing effect, the moisturizing time is short.

[0004] Therefore, it is essential to develop a plant-based moisturizing skin care water that can provide long-lasting hydration and stimulate the skin's own moisturizing properties from multiple angles.

[0005] In view of this, the present invention is hereby proposed. Summary of the Invention

[0006] One objective of this invention is to provide a plant-based moisturizing water that provides long-lasting hydration, absorbs quickly, is non-sticky, and can stimulate the skin's own moisturizing properties from multiple angles.

[0007] Another object of the present invention is to provide a method for preparing plant-based moisturizing water.

[0008] To achieve the above-mentioned objectives of the present invention, one aspect of the present invention provides a plant-based moisturizing water, comprising the following components in parts by weight:

[0009] The solvent consists of 80-96 parts, the humectant 1-10 parts, the thickener 0.01-0.3 parts, and the chelating agent 0.01-0.1 parts.

[0010] The moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (1-5):(1-5).

[0011] In a specific embodiment of the present invention, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (3.5-4.5):(3-4). Further, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of 4:3.5.

[0012] In a specific embodiment of the present invention, the Dendrobium stem extract is an aqueous extract of Dendrobium stem, and the Hydrangea extract is an aqueous extract of Hydrangea fungus.

[0013] In a specific embodiment of the present invention, the molecular weight of the Dendrobium stem extract is 10 wDa to 50 wDa.

[0014] In a specific embodiment of the present invention, the moisturizer further includes sodium hyaluronate. Further, the content of sodium hyaluronate in the moisturizer is 0.05 wt% to 0.5 wt%, preferably 0.1 wt% to 0.2 wt%.

[0015] In a specific embodiment of the present invention, the preparation of the moisturizer includes:

[0016] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized, deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m), and the filtrate was collected.

[0017] (b) The filtrate and water were used to extract *Hydrangea macrophylla* at a material-to-liquid ratio of 1:(10-100)(m / m), and the extract was then decolorized, deodorized, and filtered.

[0018] In a specific embodiment of the present invention, the preparation of the moisturizer includes:

[0019] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized and deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m). The extract was separated by membrane separation and Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa was collected.

[0020] (b) The extract of *Hydrangea macrophylla* was carried out using Dendrobium polysaccharide extract and water at a material-to-liquid ratio of 1:(10-100)(m / m). The extract was then decolorized, deodorized, and filtered.

[0021] In a specific embodiment of the present invention, the extraction temperature is 40–90°C; the extraction time is 1–4 hours.

[0022] In a specific embodiment of the present invention, the *Hydrangea macrophylla* is subjected to high-temperature micro-pressure or steam treatment before extraction.

[0023] In a specific embodiment of the present invention, the preparation of the moisturizer further includes: mixing the mixture of the Dendrobium stem extract and the Hydrangea macrophylla extract with sodium hyaluronate in a certain proportion, and then subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment; or, mixing the Dendrobium stem extract with sodium hyaluronate in a certain proportion, subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment, and then mixing it with the Hydrangea macrophylla extract.

[0024] In a specific embodiment of the present invention, the solvent comprises water and butanediol. Further, in the plant-based moisturizing water, the mass fraction of the butanediol is 2% to 4%.

[0025] In a specific embodiment of the present invention, the thickener is selected from one or more of xanthan gum, carbomer U20, carbomer 940, gum arabic, carrageenan, and hydroxyethyl cellulose.

[0026] In a specific embodiment of the present invention, the chelating agent includes disodium EDTA.

[0027] In a specific embodiment of the present invention, the preservative is further included in 0.1 to 1 parts by weight; the preservative is selected from at least one of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, methylparaben, benzyl alcohol, benzoic acid, DMDM ​​hydantoin, bis(hydroxymethyl)imidazolidinyl urea and iodopropynyl butylcarbamate.

[0028] Another aspect of the present invention provides a method for preparing plant-based moisturizing water, comprising the following steps:

[0029] (1) Disperse the thickener, chelating agent and butanediol evenly before adding water, mix and heat to 80-82°C, keep warm and defoam;

[0030] (2) After the material in step (1) is cooled to below 45°C, humectant and preservative are added in sequence and stirred evenly to obtain plant humectant water.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] (1) The moisturizing water of the present invention can effectively increase the skin's moisture content, provide long-lasting moisturization, and has a good skin feel without stickiness through the reasonable combination of components.

[0033] (2) The preparation method of the moisturizing water of the present invention is simple to operate and suitable for mass production. Attached Figure Description

[0034] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0035] Figure 1 The rheological test results are for single solutions and combined solutions of sodium hyaluronate and Dendrobium polysaccharide of the present invention.

[0036] Figure 2 The results show the moisture absorption rate of single and combined samples of sodium hyaluronate and Dendrobium polysaccharide according to the present invention. Detailed Implementation

[0037] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings and specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0038] This invention provides a plant-based moisturizing water, comprising the following components in parts by weight:

[0039] The solvent consists of 80-96 parts, the humectant 1-10 parts, the thickener 0.01-0.3 parts, and the chelating agent 0.01-0.1 parts.

[0040] The moisturizers include Dendrobium stem extract and Hydrangea extract in a mass ratio of (1-5):(1-5).

[0041] The plant-based moisturizing water of this invention, through the reasonable combination of its components, can effectively increase the skin's moisture content, provide long-lasting hydration, and has a pleasant, non-sticky feel.

[0042] In different implementation methods, the amounts of each component in the plant-based moisturizing water, by weight, can be exemplarily exemplified as follows:

[0043] The amount of solvent can be 80 parts, 82 parts, 85 parts, 88 parts, 90 parts, 92 parts, 95 parts, 96 parts, or any combination thereof;

[0044] The amount of moisturizer used can be 1 part, 2 parts, 4 parts, 5 parts, 6 parts, 8 parts, 10 parts, or any combination thereof;

[0045] The amount of thickener can be 0.01 parts, 0.1 parts, 0.2 parts, 0.3 parts, or any combination thereof;

[0046] The amount of chelating agent can be 0.01 parts, 0.03 parts, 0.05 parts, 0.07 parts, 0.09 parts, 0.1 parts, or any combination thereof.

[0047] The mass ratio of Dendrobium stem extract and Hydrangea extract in the moisturizer can be 1:1, 1:2, 1:3, 1:4, 1:5, 2:1, 2:3, 2:5, 3:1, 3:2, 3:4, 3:5, 4:1, 4:3, 4:5, 5:1, 5:2, 5:3, 5:4, or any combination thereof.

[0048] In a specific embodiment of the present invention, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of (3.5-4.5):(3-4). Further, the moisturizer comprises Dendrobium stem extract and Hydrangea macrophylla extract in a mass ratio of 4:3.5.

[0049] In the moisturizer of the present invention, the amounts of Dendrobium stem extract and Hydrangea extract are calculated based on the amounts of Dendrobium stem and Hydrangea used as raw materials in the preparation of the moisturizer.

[0050] This invention uses the above-mentioned ratio of Dendrobium stems and Hydrangea macrophylla for extraction, which helps to promote the production of hyaluronic acid endogenously, promote the expression of aquaporin AQP3, and promote the synthesis of tight junction protein Claudin-1 and filaggrin FLG, resulting in a significant long-lasting moisturizing effect.

[0051] In a specific embodiment of the present invention, the Dendrobium stem extract is an aqueous extract of Dendrobium stem, and the Hydrangea extract is an aqueous extract of Hydrangea fungus.

[0052] In a specific embodiment of the present invention, the molecular weight of Dendrobium polysaccharide in the Dendrobium stem extract is 10 wDa to 50 wDa.

[0053] In different embodiments, the molecular weight of the Dendrobium polysaccharide in the Dendrobium stem extract can be within the range of 10 wDa, 15 wDa, 20 wDa, 25 wDa, 30 wDa, 35 wDa, 40 wDa, 45 wDa, 50 wDa, or any combination thereof.

[0054] Using Dendrobium polysaccharides with molecular weights within the above range, combined with other components, results in superior moisturizing effects and skin feel.

[0055] In a specific embodiment of the present invention, the moisturizer further includes sodium hyaluronate. Further, the content of sodium hyaluronate in the moisturizer is 0.05 wt% to 0.5 wt%, preferably 0.1 wt% to 0.2 wt%.

[0056] In various embodiments, the content of sodium hyaluronate in the humectant may be a range of 0.05 wt%, 0.1 wt%, 0.15 wt%, 0.2 wt%, 0.25 wt%, 0.3 wt%, 0.35 wt%, 0.4 wt%, 0.45 wt%, 0.5 wt%, or any combination thereof.

[0057] The addition of sodium hyaluronate can form a network structure with Dendrobium polysaccharides through supramolecular interactions, which has the effects of water capture, slow release, and deep penetration.

[0058] The molecular weight of sodium hyaluronate can be adjusted according to actual needs. For example, the molecular weight of sodium hyaluronate can be 1000 Da to 200 wDa, such as 1000 Da, 5000 Da, 1 wDa, 5 wDa, 10 wDa, 20 wDa, 50 wDa, 100 wDa, 200 wDa, or any combination thereof.

[0059] In a specific embodiment of the present invention, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the Dendrobium stem extract is (1-6):1.

[0060] In different embodiments, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the Dendrobium stem extract can be 1:1, 1.5:1, 2:1, 2.5:1, 3:1, 3.5:1, 4:1, 4.5:1, 5:1, 5.5:1, 6:1, or any combination thereof.

[0061] When the content of sodium hyaluronate in the moisturizer meets the above requirements, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the Dendrobium stem extract is (1-6):1. For example, when the content of sodium hyaluronate is 0.15wt%, the mass ratio of Dendrobium polysaccharide to sodium hyaluronate in the obtained moisturizer is close to 5:2.

[0062] The Dendrobium used in this invention includes Dendrobium officinale.

[0063] In a specific embodiment of the present invention, the preparation of the humectant includes:

[0064] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized, deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m), and the filtrate was collected.

[0065] (b) The filtrate and water were used to extract *Hydrangea macrophylla* at a material-to-liquid ratio of 1:(10-100)(m / m), and the extract was then decolorized, deodorized, and filtered.

[0066] In a specific embodiment of the present invention, the preparation of the moisturizer includes:

[0067] (a) Using water as the extraction solvent, the Dendrobium stems were extracted, decolorized and deodorized, and filtered at a material-to-liquid ratio of 1:(10-80)(m / m). The extract was separated by membrane separation and Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa was collected.

[0068] (b) The extract of *Hydrangea macrophylla* was carried out using Dendrobium polysaccharide extract and water at a material-to-liquid ratio of 1:(10-100)(m / m). The extract was then decolorized, deodorized, and filtered.

[0069] In different embodiments, in step (a), the feed-liquid ratio can be 1:10 (m / m), 1:20 (m / m), 1:30 (m / m), 1:40 (m / m), 1:50 (m / m), 1:60 (m / m), 1:70 (m / m), 1:80 (m / m), or any combination thereof; in step (b), the feed-liquid ratio can be 1:10 (m / m), 1:20 (m / m), 1:30 (m / m), 1:40 (m / m), 1:50 (m / m), 1:60 (m / m), 1:70 (m / m), 1:80 (m / m), 1:90 (m / m), 1:100 (m / m), or any combination thereof.

[0070] In step (b), Dendrobium polysaccharide extract (or filtrate) and water are used together as extraction solvents to extract Hydrangea macrophylla. The material-liquid ratio refers to the ratio of the mass of Hydrangea macrophylla relative to the mass of Dendrobium polysaccharide extract (or filtrate) and water.

[0071] In another specific embodiment of the present invention, the preparation of the moisturizer includes: extracting Dendrobium stems and Hydrangea macrophylla separately using water as the extraction solvent and a material-to-liquid ratio of 1:(10-100)(m / m); decolorizing and deodorizing the extract and filtering it; and then mixing and compounding the Dendrobium stem extract and Hydrangea macrophylla extract.

[0072] In a specific embodiment of the present invention, the extraction temperature is 40–90°C; the extraction time is 1–4 hours.

[0073] In different implementations, the extraction temperature can be a range of 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C or any combination thereof, and the extraction time can be a range of 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h or any combination thereof.

[0074] In a specific embodiment of the present invention, decolorization and deodorization include: treating the extract with activated carbon or hydrogen peroxide, and collecting the filtrate. Further, the amount of activated carbon added is 0.1 wt% to 2 wt% of the extract; the amount of hydrogen peroxide added is 0.01 wt% to 1 wt% of the extract.

[0075] In a specific embodiment of the present invention, the filtration process includes coarse filtration and fine filtration. Further, the coarse filtration includes paperboard filtration; the fine filtration includes both paperboard filtration and diatomaceous earth filtration.

[0076] In a specific embodiment of the present invention, the *Hydrangea macrophylla* is subjected to high-temperature micro-pressure or steam treatment before extraction. Further, the high-temperature micro-pressure treatment includes: a temperature of 110–121°C, a pressure of 0.15–0.23 MPa, and a micro-pressure time of 5–20 min; the steam treatment includes: steaming at 90–100°C for 5–20 min. Wherein, the pressure in the high-temperature micro-pressure treatment refers to absolute pressure, which is the sum of gauge pressure (relative pressure) and atmospheric pressure.

[0077] In different embodiments, the high-temperature micro-pressure treatment can be performed at temperatures ranging from 110°C, 112°C, 114°C, 115°C, 116°C, 118°C, 120°C, 121°C, or any combination thereof; the pressure can be ranging from 0.15MPa, 0.16MPa, 0.18MPa, 0.2MPa, 0.22MPa, 0.23MPa, or any combination thereof; and the treatment time can be ranging from 5min, 8min, 10min, 12min, 15min, 18min, 20min, or any combination thereof.

[0078] High-temperature micro-pressure or steam treatment helps to promote the extraction of active ingredients from *Hydrangea macrophylla*, thereby further enhancing the moisturizing ability of the resulting moisturizer.

[0079] In a specific embodiment of the present invention, the preparation of the moisturizer further includes: mixing a mixture of Dendrobium stem extract and Hydrangea macrophylla extract with sodium hyaluronate in a certain proportion, and then subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment; or, mixing Dendrobium stem extract with sodium hyaluronate in a certain proportion, subjecting it to homogenization, microwave, ultrasonic and / or micro-pressure treatment, and then mixing it with Hydrangea macrophylla extract.

[0080] The homogenization conditions may include: a rotation speed of 2000–5000 rpm and a homogenization time of 5–30 min; the micro-pressure conditions may include: a temperature of 110–121℃, a pressure of 0.15–0.23 MPa, and a micro-pressure time of 5–20 min; the microwave conditions may include: a microwave power of 200–800 W and a microwave time of 5–20 min; and the ultrasonic conditions may include: an ultrasonic power of 50–400 W, an ultrasonic temperature of 30–60℃, and an ultrasonic time of 5–20 min. The pressure in the micro-pressure treatment refers to absolute pressure, which is the sum of gauge pressure (relative pressure) and atmospheric pressure.

[0081] In a specific embodiment of the present invention, the solvent includes water and butanediol. Further, in the plant-based moisturizing water, the mass fraction of butanediol is 2% to 4%.

[0082] In different embodiments, the mass fraction of butanediol in the plant-based moisturizing water can be 2%, 2.2%, 2.5%, 2.8%, 3%, or any combination thereof. The butanediol may be 1,3-butanediol.

[0083] In a specific embodiment of the present invention, the thickener is selected from at least one of xanthan gum, carbomer U20, carbomer 940, gum arabic, carrageenan, and hydroxyethyl cellulose.

[0084] In a specific embodiment of the present invention, the chelating agent includes disodium EDTA.

[0085] In a specific embodiment of the present invention, it may further include 0.1 to 1 part by weight of preservative; the preservative is selected from at least one of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, methylparaben, benzyl alcohol, benzoic acid, DMDM ​​hydantoin, bis(hydroxymethyl)imidazolidinyl urea and iodopropynyl butylcarbamate.

[0086] In different embodiments, the amount of preservative can be 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, or any combination thereof.

[0087] Another aspect of the present invention provides a method for preparing plant-based moisturizing water, comprising the following steps:

[0088] (1) Disperse the thickener, chelating agent and butanediol evenly before adding water, mix and heat to 80-82°C, keep warm and defoam;

[0089] (2) After the material in step (1) is cooled to below 45°C, humectant and preservative are added in sequence and stirred evenly to obtain plant humectant water.

[0090] In a specific embodiment of the present invention, in step (1), the heat preservation time is 20 to 30 minutes.

[0091] The raw material information used in the following specific embodiments may be as follows, but is not limited to:

[0092] 1,3-Butanediol: Supplier: Dow Chemical; Aloe Vera Extract: Supplier: Beijing Dongfang Miaosen Biotechnology Co., Ltd.; Carbopol U20, Carbopol 940: Supplier: Lubrizol; Xanthan Gum: Supplier: Rhodia; Disodium EDTA: Supplier: AkzoNobel; 1,2-Hexanediol: Supplier: Rentai; 1,2-Pentanediol: Supplier: Huayu; Phenoxyethanol and Ethylhexylglycerin: Supplier: THOR.

[0093] Examples 1-7

[0094] Examples 1-7 of this invention provide plant-based moisturizing water and its preparation method. The raw material composition of the plant-based moisturizing water is shown in Table 1. The specific preparation method includes the following steps:

[0095] (1) Disperse the thickener, chelating agent and 1,3-butanediol evenly before adding water, mix and heat to 80-82℃, keep warm for 20 minutes to sterilize and defoam, and set aside;

[0096] (2) When the material from step (1) is cooled to below 45°C, add the humectant and preservative in sequence and stir thoroughly to obtain plant humectant water.

[0097] Table 1. Raw material composition (parts by weight) of different plant-based moisturizing waters

[0098]

[0099]

[0100] The first preservative includes 1,2-hexanediol and p-hydroxyacetophenone in a mass ratio of 1:1.

[0101] The second preservative consists of phenoxyethanol and ethylhexylglycerin in a mass ratio of 9:1.

[0102] The preparation methods of the first moisturizer include:

[0103] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0104] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter and collect the filtrate.

[0105] (c) Membrane separation: The filtrate obtained in step (b) is filtered using 10wDa and 50wDa membranes, and the Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa is collected.

[0106] (d) Add sodium hyaluronate to the Dendrobium polysaccharide extract obtained in step (c) to obtain a compound solution; homogenize the compound solution in a homogenizer at 2500 rpm for 10 min.

[0107] (e) Extraction of Hydrangea macrophylla: Hydrangea macrophylla was mixed at a material-to-liquid ratio of 1:60 (m / m), stirred at room temperature for 30 min, and then extracted at 60℃ for 2 h. After extraction, 0.5 wt% activated carbon was added for decolorization and deodorization treatment, followed by fine filtration and collection of filtrate.

[0108] (f) Compounding: The compound solution after homogenization in step (d) is mixed with the filtrate obtained in step (e), and 23% glycerol (mass fraction of the mixture) is added to obtain the mixture.

[0109] (g) Sterilization: Add 1% 1,2-pentanediol and 1.5% 1,2-hexanediol (by mass fraction of the final product) to the material obtained in step (f), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0110] The preparation of the second moisturizer includes:

[0111] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0112] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter and collect the filtrate.

[0113] (c) Membrane separation: The filtrate obtained in step (b) is filtered using 10wDa and 50wDa membranes, and the Dendrobium polysaccharide extract with a molecular weight between 10w and 50wDa is collected.

[0114] (d) Add a certain amount of water to the Dendrobium polysaccharide extract obtained in step (c) (use all of the Dendrobium polysaccharide extract and make up the rest with water), and mix it with Hydrangea macrophylla at a material-to-liquid ratio of 1:60 (m / m). Stir at room temperature for 30 minutes, and then extract at 60℃ for 2 hours. After extraction, add 0.5wt% activated carbon for decolorization and deodorization, and then filter with gauze and collect the filtrate.

[0115] (e) Filtration: The filtrate obtained in step (d) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0116] (f) Compounding: Add a certain amount of glycerol to the filtrate obtained in step (e) to obtain a compound solution.

[0117] (g) Sterilization: Add 1,2-pentanediol and 1,2-hexanediol to the compound solution in step (f), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0118] In step (f), the mass fraction of glycerol in the compound solution is 23%; in step (g), the mass fractions of 1,2-pentanediol and 1,2-hexanediol in the final product are 1% and 1.5%, respectively.

[0119] Example 8

[0120] This embodiment provides a plant-based moisturizing water and its preparation method. Referring to Embodiment 2, the only difference is that the first moisturizing agent is replaced with an equal weight of a third moisturizing agent.

[0121] The preparation method of the third moisturizer used in this embodiment includes:

[0122] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0123] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter with gauze and collect the filtrate.

[0124] (c) Add a certain amount of water to the filtrate obtained in step (b) (use all the filtrate and make up the rest with water), and stir with *Hydrangea macrophylla* at a material-to-liquid ratio of 1:60 (m / m) for 30 minutes at room temperature, and then extract at 60℃ for 2 hours. After extraction, add 0.5wt% activated carbon for decolorization and deodorization, and then filter with gauze to collect the filtrate.

[0125] (d) Filtration: The filtrate obtained in step (c) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0126] (e) Compounding: Add a certain amount of glycerol to the filtrate obtained in step (d) to obtain a compound solution.

[0127] (f) Sterilization: Add 1,2-pentanediol and 1,2-hexanediol to the compound solution in step (e), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0128] In step (e), the mass fraction of glycerol in the compound solution is 23%; in step (f), the mass fractions of 1,2-pentanediol and 1,2-hexanediol in the final product are 1% and 1.5%, respectively.

[0129] Example 9

[0130] This embodiment provides a plant-based moisturizing water and its preparation method. Referring to Embodiment 2, the only difference is that the first moisturizing agent is replaced with an equal weight of a fourth moisturizing agent.

[0131] The preparation methods of the fourth moisturizer include:

[0132] (a) Raw material pretreatment: Weigh out the hydrangea fungus and treat it under high temperature and micro pressure (110℃, 20min), and crush the Dendrobium officinale stem through an 80-mesh sieve; the mass ratio of Dendrobium officinale stem to hydrangea fungus is 4:3.5.

[0133] (b) Extraction: Weigh Dendrobium officinale stems and add pure water at a ratio of 1:40 (m / m) for material to liquid. Stir at room temperature for 30 min and then extract at 60℃ for 2 h. After extraction, add 0.5 wt% activated carbon for decolorization and deodorization treatment, then filter with gauze and collect the filtrate.

[0134] (c) Membrane separation: The filtrate obtained in step (b) is filtered using 1wDa and 10wDa membranes to collect Dendrobium polysaccharide extracts with molecular weights between 1w and 10wDa.

[0135] (d) Add a certain amount of water to the Dendrobium polysaccharide extract obtained in step (c) (use all of the Dendrobium polysaccharide extract and make up the rest with water), and mix it with Hydrangea macrophylla at a material-to-liquid ratio of 1:60 (m / m). Stir at room temperature for 30 minutes, and then extract at 60℃ for 2 hours. After extraction, add 0.5wt% activated carbon for decolorization and deodorization, and then filter with gauze and collect the filtrate.

[0136] (e) Filtration: The filtrate obtained in step (d) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0137] (f) Compounding: Add a certain amount of glycerol and sodium hyaluronate to the filtrate obtained in step (d) to obtain a compound solution.

[0138] (g) Sterilization: Add 1,2-pentanediol and 1,2-hexanediol to the compound solution in step (f), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0139] In step (f), the compound solution contains 23% glycerol and 0.15% sodium hyaluronate by mass; in step (g), 1,2-pentanediol and 1,2-hexanediol account for 1% and 1.5% of the final product by mass, respectively.

[0140] Example 10

[0141] This embodiment provides a plant-based moisturizing water and its preparation method. Referring to Embodiment 2, the only difference is that the first moisturizing agent is replaced with an equal weight of the fifth moisturizing agent.

[0142] The only difference between the preparation methods of the fifth and fourth moisturizers is step (c).

[0143] In the preparation of the fifth moisturizer, step (c) is as follows: the filtrate obtained in step (b) is filtered using 1000Da and 1wDa membranes, and the Dendrobium polysaccharide extract with a molecular weight between 1000Da and 1wDa is collected.

[0144] Comparative Example 1

[0145] Comparative Example 1 provides a moisturizing water and its preparation method. Referring to Example 2, the difference is that the first moisturizer is replaced with an equal weight of a sixth moisturizer.

[0146] The sixth moisturizer consists of betaine and glycerin in a mass ratio of 1:4.

[0147] Comparative Example 2

[0148] Comparative Example 2 provides a moisturizing water and its preparation method, referring to Example 2, except that the first moisturizer is replaced with an equal weight of a seventh moisturizer.

[0149] The seventh moisturizer is aloe vera extract.

[0150] Comparative Examples 3-6

[0151] Comparative Examples 3-6 provide moisturizing water and its preparation method, referring to Example 1, except that the raw material composition of the moisturizing water is different from that of Example 2.

[0152] The raw material composition of the moisturizing waters in Comparative Examples 3 to 6 is shown in Table 2.

[0153] Table 2. Raw material composition (parts by weight) of different moisturizing waters

[0154]

[0155] Comparative Example 7

[0156] Comparative Example 7 provides a moisturizing water and its preparation method, referring to Example 2, except that the first moisturizer is replaced with an equal weight of an eighth moisturizer.

[0157] The preparation of the eighth moisturizer includes:

[0158] (a) Raw material pretreatment: Weigh the Dendrobium officinale stems, crush them and pass them through an 80-mesh sieve.

[0159] (b) Extraction: Dendrobium officinale stems were added to pure water at a ratio of 1:40 (m / m) and stirred at room temperature for 30 min. Then, they were extracted at 60℃ for 2 h. After extraction, 0.5% activated carbon was added for decolorization. The solution was then filtered through gauze and the filtrate was collected.

[0160] (c) Filtration: The filtrate obtained in step (b) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0161] (d) Compounding: Add 23% glycerol (mass fraction of compounded solution) to the filtrate obtained in step (c) to obtain compounded solution.

[0162] (e) Sterilization: Add 1% pentanediol and 1.5% hexanediol (by mass fraction of the final product) to the compound solution in step (d), and sterilize by stirring at 98-100°C for 40 minutes. After cooling, fill the package.

[0163] Comparative Example 8

[0164] Comparative Example 8 provides a moisturizing water and its preparation method, referring to Example 2, except that the first moisturizer is replaced with an equal weight of the ninth moisturizer.

[0165] The preparation of the ninth moisturizer includes:

[0166] (a) Raw material pretreatment: Weigh out the *Hydrangea macrophylla* and treat it under high temperature and micro pressure (110℃, 20min).

[0167] (b) Extraction: Add the *Hydrangea macrophylla* to pure water at a ratio of 1:60 (m / m), stir at room temperature for 30 min, and then extract at 60℃ for 2 h. After extraction, add 0.5% activated carbon for deodorization treatment, and then filter with gauze to collect the filtrate.

[0168] (c) Filtration: The filtrate obtained in step (b) is filtered using F100 paperboard to collect the coarse filtrate; then the coarse filtrate is cooled to below 35°C and filtered using H70 paperboard + diatomaceous earth to collect the fine filtrate.

[0169] (d) Compounding: Add 23% glycerol (mass fraction of compounded solution) to the filtrate obtained in step (c) to obtain compounded solution.

[0170] (e) Sterilization: Add 1% pentanediol and 1.5% hexanediol (by mass fraction of the final product) to the compound solution in step (d), stir and sterilize at 98-100°C for 40 min, and fill after cooling.

[0171] Experimental Example 1

[0172] (1) Heat resistance test

[0173] The moisturizing water of the examples and comparative examples was kept at 45±2℃ for 1 month, 2 months and 3 months. After returning to room temperature, the turbidity was observed as before the test. ○ indicates no turbidity; × indicates turbidity.

[0174] (2) Cold resistance test

[0175] The moisturizing water of the examples and comparative examples was kept at -17±2℃ for 1 month, 2 months and 3 months. After returning to room temperature, it was observed whether there was any significant difference in properties compared with before the test. ○ indicates no difference; × indicates difference.

[0176] (3) Light stability test

[0177] The moisturizing water of the examples and comparative examples was placed in a light incubator with strong light conditions of (4500±500) lx for 1 month, 2 months and 3 months. After returning to room temperature, it was observed whether there was obvious color change compared with before the experiment. ○ indicates no difference; × indicates difference.

[0178] Table 3 Results of heat resistance, cold resistance and irradiation stability tests

[0179]

[0180]

[0181] As can be seen from the above test results, the moisturizing water of the present invention showed no significant changes before and after the tests for cold resistance, heat resistance, and light stability, and all properties were good.

[0182] Experimental Example 2

[0183] Human body moisturizing effect test

[0184] Fifteen volunteers were selected and divided into three groups. Moisturizing lotions (Examples 1-10 and Comparative Examples 1-8) were applied to different areas of the flexor surface of the volunteers' forearms. The moisture content of the corresponding areas of the flexor surface of the volunteers' forearms was measured using a Corneometer skin moisture content analyzer before application and at 1 hour, 2 hours, 4 hours, and 8 hours after application. Higher skin moisture content values ​​indicate higher skin hydration and better moisturizing effect. The test results are shown in Table 4.

[0185] Table 4 Results of Human Body Moisturizing Efficacy Test

[0186]

[0187]

[0188] As can be seen from the above test results, the moisturizing water of the present invention can significantly increase the skin's moisture content in a timely manner, and can effectively maintain the skin's moisture content after 8 hours of use.

[0189] Sensory evaluation

[0190] Volunteers: 20 volunteers aged 18-40, each using all samples and evaluating each sample separately.

[0191] Application area: Arm

[0192] Test samples: Examples 1-10, Comparative Examples 1-8

[0193] How to use: Apply 0.25mL of the corresponding moisturizing water to different parts of the arm. The results are evaluated on a 5-point scale to assess the skin feel after use.

[0194] 4-5 points: The feeling is very obvious;

[0195] 3-4 points: The feeling is quite obvious;

[0196] 2-3 points: Mild sensation;

[0197] 1-2 points: No relevant feelings.

[0198] The test results are shown in Table 5.

[0199] Table 5 Sensory evaluation results

[0200]

[0201] Sensory test results show that the plant-based moisturizing water of the present invention, with the addition of 1% of moisturizing plant extracts as a moisturizing agent, can achieve a good lubrication and moisturizing feel, and is absorbed quickly without stickiness.

[0202] Experimental Example 3

[0203] This invention utilizes membrane separation technology to separate and purify Dendrobium polysaccharides of different molecular weights from Dendrobium extract, yielding four samples: 10w–50wDa, 1w–10wDa, 1000Da–1wDa, and whole-fraction (not separated). These correspond to the Dendrobium polysaccharide extracts obtained in step (c) of the first humectant treatment, the fourth humectant treatment, the fifth humectant treatment, and the filtrate obtained in step (b) of the first humectant treatment, respectively. Through AQP3 gene expression efficacy testing and skin sensitivity evaluation, the optimal Dendrobium polysaccharide molecular fragments in terms of efficacy and skin sensitivity are selectively identified.

[0204] 1. AQP3 gene expression test

[0205] This experiment first used the MTT assay to detect cell viability and screen the maximum safe concentration for cell sample administration. The expression levels of each gene were then detected by quantitative real-time PCR. The test groupings and results are shown in Tables 6 and 7, respectively. The sample preparation method included adding 0.00781 wt% of each Dendrobium officinale polysaccharide extract to the culture medium and heating and stirring at 55–60°C for 10–15 min.

[0206] Table 6 AQP3 Test Grouping

[0207]

[0208] Table 7. Test results of AQP3 in Dendrobium polysaccharides of different molecular weights

[0209]

[0210]

[0211] The test results above show that Dendrobium polysaccharides with molecular weights of 10w-50wDa and 1w-10wDa can promote AQP3 gene expression, with Dendrobium polysaccharides with molecular weights of 10w-50wDa showing better effects.

[0212] 2. Skin feel test

[0213] Different molecular weight Dendrobium polysaccharide extracts: 10w–50wDa (Sample 1), 1w–10wDa (Sample 2), 1000Da–1wDa (Sample 3), and the whole segment (Sample 4) were each prepared into 5wt% aqueous solutions. Eight testers applied the extracts and evaluated their smoothness and other skin feel, ranking them by preference. The specific preparation of the aqueous solutions involved adding each molecular weight Dendrobium polysaccharide extract at 5wt% to water and stirring for 10–15 minutes. The test results are shown in Table 8.

[0214] Table 8. Skin feel test results of Dendrobium polysaccharides with different molecular weights

[0215] Trial user serial number Preference 1 1＞4＞2＞3 2 1＞4＞2＞3 3 1＞4＞2＞3 4 1＞4＞2＞3 5 1＞4＞2＞3 6 1＞4＞2＞3 7 1＞4＞2＞3 8 1＞4＞2＞3

[0216] The test results above show that all eight testers agreed that the 10w-50wDa Dendrobium polysaccharide had the best smoothness, followed by the whole-section sample, with significant differences between the samples. In conclusion, the 10w-50wDa Dendrobium polysaccharide has superior moisturizing effect and skin feel.

[0217] Experiment Example 4

[0218] Verification of supramolecular interactions

[0219] Rheological test:

[0220] Test sample:

[0221] 0.5% HA: i.e., 0.5 wt% sodium hyaluronate aqueous solution;

[0222] 0.5% Dendrobium polysaccharide: i.e., 0.5wt% of an aqueous solution of Dendrobium polysaccharide (lyophilized powder of Dendrobium polysaccharide extract obtained in step (c) of the first humectant step) with a molecular weight of 10w~50wDa;

[0223] 0.5% Dendrobium polysaccharide + HA solution mixed sample: i.e. 0.5wt% (sodium hyaluronate + Dendrobium polysaccharide with a molecular weight of 10w~50wDa) aqueous solution (the mass ratio of Dendrobium polysaccharide to HA is 5:2, and Dendrobium polysaccharide is the first humectant, and the Dendrobium polysaccharide is the freeze-dried powder of Dendrobium polysaccharide extract obtained in step (c));

[0224] 0.5% Dendrobium polysaccharide-HA supramolecular polysaccharide assembly sample: The lyophilized powder of 10w~50wDa Dendrobium polysaccharide extract obtained in step (c) of the first moisturizing agent was compounded with HA at a mass ratio of 5:2 and dissolved in water. After homogenization in a homogenizer (2500rpm, 10min), the product was lyophilized to obtain the Dendrobium polysaccharide-HA supramolecular polysaccharide assembly product. Then, the product was prepared into a 0.5wt% aqueous solution.

[0225] In the preparation of the above samples, they were dispersed and dissolved in distilled water at the corresponding concentration, heated and stirred in a water bath at 40°C for 1 hour, and then placed at room temperature and shaken in a shaker at room temperature for 4 hours.

[0226] Rheological tests were performed on the above samples to investigate the viscosity differences between different samples and to verify the existence of supramolecular interactions. The test results are shown in […]. Figure 1 .

[0227] The rheological test results show that both the Dendrobium polysaccharide solution and the HA solution have very low viscosity. However, the viscosity increases significantly when they are combined. The viscosity of the Dendrobium polysaccharide-HA supramolecular polysaccharide assembly solution is higher than that of HA or Dendrobium polysaccharide single solutions at the same concentration. Therefore, there is a synergistic effect between Dendrobium polysaccharide and HA molecules, which intensifies the polymerization of hydrogen bonds between polysaccharide molecules. This results in the viscosity of the Dendrobium polysaccharide-HA supramolecular system being higher than that of HA or Dendrobium polysaccharide single polysaccharide solutions at the same concentration, thus exhibiting a thickening effect. Solution mixing also has a thickening effect, but it is not as strong as that of supramolecular polysaccharides, indicating that the supramolecular polysaccharide assembly has strong interactions.

[0228] Enhanced efficacy of supramolecular combination

[0229] This study investigated the hygroscopic effects of Dendrobium polysaccharide, sodium hyaluronate, a mixture of Dendrobium polysaccharide and sodium hyaluronate solutions, and Dendrobium polysaccharide-HA supramolecular polysaccharide assembly samples under high humidity conditions through in vitro hygroscopic tests, thus verifying the efficacy of supramolecular samples.

[0230] Sample 1: Sodium hyaluronate;

[0231] Sample 2: Dendrobium polysaccharide with a molecular weight of 10w-50wDa (the Dendrobium polysaccharide extract obtained in step (c) of the first moisturizing agent was dried);

[0232] Sample 3: Dendrobium polysaccharide + HA solution mixture (the freeze-dried powder of Dendrobium polysaccharide extract obtained in step (c) of the first moisturizing agent was mixed with HA in solution at a mass ratio of 5:2 and then dried);

[0233] Sample 4: Dendrobium polysaccharide-HA supramolecular polysaccharide assembly (the freeze-dried powder of Dendrobium polysaccharide extract obtained in step (c) of the first moisturizing agent was compounded with HA at a mass ratio of 5:2 and dissolved in water, homogenized in a homogenizer (2500 rpm, 10 min) and then dried).

[0234] The above samples (set up in triplicate) were dried to constant weight and then placed in a desiccator containing a saturated ammonium sulfate solution (theoretical relative humidity 85%). Their mass was measured and recorded at set time points of 0, 2, 4, and 8 hours. The moisture absorption rate of the samples was determined based on their mass before and after drying. The test results are shown below. Figure 2 .from Figure 2It can be seen that within 8 hours, the moisture absorption effect of sample 4 is significantly better than that of samples 1, 2 and 3, that is, the supramolecular samples of Dendrobium polysaccharide and sodium hyaluronate have a synergistic effect.

[0235] The test results above show that the moisturizer used in this invention has a synergistic effect when Dendrobium polysaccharide and sodium hyaluronate are assembled through supramolecular polysaccharide. While precisely promoting the high expression of AQP3, it forms a "DHA network" to achieve water capture, slow release and deep penetration.

[0236] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant moisturizing water, characterized by, It comprises the following components by weight parts: The solvent 80~96 parts, humectant 1~10 parts, thickening agent 0.01~0.3 parts and chelating agent 0.01~0.1 parts; The humectant includes the stem extract of Dendrobium candidum and the extract of Galanthus by mass ratio (1~5) :(1~5); The humectant also includes sodium hyaluronate; The stem extract of Dendrobium candidum is water extract of Dendrobium candidum stem, and the extract of Galanthus is water extract of Galanthus; The molecular weight of Dendrobium polysaccharide in the stem extract of Dendrobium candidum is 10wDa~50wDa; The preparation of the humectant comprises: (a) using water as the extraction solvent, the ratio of material to liquid is 1 :(10~80), the stem of Dendrobium candidum is extracted, decolorized and deodorized, filtered, and the extract is separated by membrane separation, and the Dendrobium polysaccharide extract with molecular weight of 10w~50wDa is collected; (b) the Galanthus is extracted with water under the condition of 1 :(10~100) of the ratio of material to liquid, and then the extract is treated by decolorization, deodorization and filtration to obtain the extract of Galanthus; (c) the Dendrobium polysaccharide extract and sodium hyaluronate are mixed in proportion, homogenized, and then mixed with the extract of Galanthus; The ratio of material to liquid is mass ratio; Before the extraction, the Galanthus is treated by high temperature and micro pressure; The high temperature and micro pressure treatment comprises: the temperature is 110~121℃, the pressure is 0.15~0.23MPa, and the micro pressure time is 5~20min.

2. The plant moisturizing water according to claim 1, characterized by, The humectant comprises the stem extract of Dendrobium candidum and the extract of Galanthus by mass ratio (3.5~4.5) :(3~4).

3. The plant moisturizing water according to claim 1, characterized by, The humectant comprises the stem extract of Dendrobium candidum and the extract of Galanthus by mass ratio 4:3.

5.

4. The plant moisturizing water according to claim 1, characterized by, The content of sodium hyaluronate in the humectant is 0.05wt%~0.5wt%.

5. The plant moisturizing water according to claim 1, characterized by, The content of sodium hyaluronate in the humectant is 0.1wt%~0.2wt%.

6. The plant moisturizing water according to claim 1, characterized by, The extraction temperature is 40~90℃; The extraction time is 1~4h.

7. The plant moisturizing water according to claim 1, characterized by, The solvent comprises water and butanediol.

8. The plant moisturizing water according to claim 7, characterized by, The mass fraction of butanediol in the plant moisturizing water is 2%~4%.

9. The plant moisturizing water according to claim 1, characterized by, The thickening agent comprises at least one of xanthan gum, carboxy U20, carboxy 940, gum arabic, chondrus crispus and hydroxyethyl cellulose; And / or, the chelating agent comprises disodium EDTA; And / or, it further comprises a preservative 0.1~1 parts by weight; The preservative is selected from at least one of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, hydroxybenzoic acid, benzyl alcohol, benzoic acid, DMDM hydantoin, bis(hydroxymethyl)imidazole alkyl urea and iopropynyl butylcarbamate.

10. The method for preparing the plant moisturizing water according to any one of claims 1 to 9, characterized by, It comprises the following steps: (1) the thickening agent, chelating agent and butanediol are uniformly dispersed in advance, then water is added and heated to 80~82℃, and then the temperature is kept constant and defoaming; (2) after the material of step (1) is cooled to below 45℃, the humectant is added and stirred uniformly to obtain the plant moisturizing water.

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