A nucleic acid cleavage amplification device

Abstract

The application discloses a nucleic acid cleavage amplification device. The nucleic acid cleavage amplification device comprises a pushing device, a liquid storage device and a chip. The liquid storage device is internally provided with multiple cavities, the pushing device extends into the interior of the liquid storage device, and the pushing device is slidably connected with the liquid storage device below. One end of the liquid storage device is snap-connected with the upper portion of the pushing device, and the other end of the liquid storage device is snap-connected with the chip. The liquid storage device is connected with the chip through a liquid inlet channel. The chip comprises a chip body, a quantitative groove, a waste liquid groove and a positioning support column. Multiple quantitative grooves and waste liquid grooves are arranged, the quantitative grooves and the waste liquid grooves are arranged on the chip body, the quantitative grooves and the waste liquid grooves are connected with the liquid storage device through quantitative channels and waste liquid channels respectively, and the positioning support column is arranged on one side of the bottom of the chip body. The chip further comprises a buffer channel, one end of the buffer channel is connected with the quantitative channel, the other end of the buffer channel is connected with the quantitative groove, and the cross-sectional area of the buffer channel is smaller than that of the quantitative channel, so that the accuracy of nucleic acid detection is improved.

Claims

1. A nucleic acid lysis and amplification device, characterized in that, include: The device comprises a pusher (1), a liquid storage device (2), and a chip (3); the liquid storage device (2) has multiple chambers inside, the pusher (1) extends into the liquid storage device (2), and the pusher (1) is slidably connected to the liquid storage device (2) from below; one end of the liquid storage device (2) is snapped to the top of the pusher (1), and the other end of the liquid storage device (2) is snapped to the chip (3); The liquid storage device (2) is connected to the chip (3) through the liquid inlet channel (4); The chip (3) includes: a chip body (31), a metering tank (32), a waste liquid tank (33), and a positioning support column (34). Multiple metering tanks (32) and waste liquid tanks (33) are provided. The metering tanks (32) and waste liquid tanks (33) are opened on the chip body (31). The metering tanks (32) and waste liquid tanks (33) are connected to the liquid storage device (2) through metering channels (5) and waste liquid channels (6), respectively. The positioning support column (34) is located on one side of the bottom of the chip body (31). The chip (3) further includes a buffer channel (7), one end of which is connected to the metering channel (5) and the other end is connected to the metering tank (32). The cross-sectional area of ​​the buffer channel (7) is smaller than that of the metering channel (5). During the heating process, the liquid in the buffer channel will quickly vaporize to form an air column, which will block the metering tank (32) from the upstream liquid.

2. The nucleic acid lysis and amplification device according to claim 1, characterized in that, The pushing device (1) includes: a pressing cover (11), a push rod (12) and a piston (13). The piston (13) is fixedly connected to the tail of the push rod (12). The piston (13) extends into the liquid storage device (2). The piston (13) is slidably connected to the liquid storage device (2) so that one end of the liquid storage device (2) is sealed. The press cover (11) is above the push rod (12), and the press cover (11) is snapped into the liquid storage device (2).

3. The nucleic acid lysis and amplification device according to claim 2, characterized in that, Also includes: A limiting device (8) is provided between the push rod (12) and the piston (13).

4. The nucleic acid lysis and amplification device according to claim 1, characterized in that, The chip (3) further includes: a vent (36), wherein a plurality of vents (36) are provided; the vents (36) are connected to the metering tank (32) and the waste liquid tank (33) respectively through flow channels.

5. The nucleic acid lysis and amplification device according to claim 1, characterized in that, The inlet channel (4) is provided with a T-shaped inlet. The metering tank (32) and the waste liquid tank (33) are connected to the T-shaped inlet through the metering channel (5) and the waste liquid channel (6) respectively. The waste liquid channel (6) and the inlet channel (4) are at right angles. The cross-sectional area of ​​the quantitative flow channel (5) is larger than that of the waste liquid flow channel (6).

Images