Pesticide composition for preventing and treating ginger root rot and application thereof

Abstract

The invention discloses a pesticide composition for preventing and treating ginger root rot and application thereof, and belongs to the technical field of chemical pesticides, and the pesticide composition for preventing and treating ginger root rot comprises hymexazol, metalaxyl-M and ethylicin. The invention provides a pesticide composition for preventing and treating ginger root rot, the pesticide composition comprises hymexazol, metalaxyl-M and ethylicin, the composition is prepared into a compound bactericide, and a suspending agent can effectively prevent and treat the ginger root rot.

Description

Technical Field

[0001] The invention belongs to the technical field of chemical pesticides, and particularly relates to a pesticide composition for preventing and treating ginger root rot and application thereof. Background Art

[0002] Root rot is a soil-borne disease, mainly caused by fungi in the soil (such as Phytophthora, Pythium and Fusarium, etc.) infecting the roots of crops. After the pathogens invade the plants through the root wounds, the roots rot and affect the absorption of nutrients and water by the plant roots. As the disease develops, the upper part of the plant cannot absorb enough nutrients and water, and the normal physiological metabolism process is affected, eventually leading to the death of the whole plant.

[0003] Crops that suffer from root rot include but are not limited to strawberries, beets, beans, cotton, peanuts, cucumbers, tomatoes, fruit trees, watermelons, melons, etc. Root rot of different crops may show different symptoms and severity due to the type of pathogen, environmental conditions and the resistance of the crop itself. Although root rot of different crops may be caused by similar pathogens, due to the physiological differences between crops and the specificity of pathogens, the use of the same agent to prevent and control different crops is not always effective. Therefore, when choosing an agent, it is necessary to comprehensively consider the disease situation of the specific crop, the type of pathogen, and the scope of application and effect of the agent.

[0004] Ginger root rot is a serious soil-borne disease in ginger production and cultivation. It is mainly caused by pathogens in ginger seeds or in the soil, and is spread through water, fertilizer, soil and farming operations. After the pathogen infects the plant, it causes the underground rhizomes to rot, and eventually causes the plant to wither and die. Once the disease occurs, it spreads rapidly. Mildly infected fields can cause a 20%-40% reduction in production, and severe cases can reach 50%-80%, or even a total loss of production. It is reported that the dominant species of root rot pathogens in my country's ginger-producing areas are Pythium and Fusarium, which are widely distributed and highly pathogenic.

[0005] Currently, there are no ginger varieties that are resistant to root rot in production, and the use of fungicides is still an important measure to effectively prevent and control the occurrence of ginger root rot. However, in actual production, it has been found that some chemical agents commonly used to prevent and control ginger root rot have caused resistance problems in pathogenic bacteria, and long-term continuous use of the same or the same type of chemical agents can easily reduce the prevention and control effect of the agents. In recent years, the incidence of root rot in my country's ginger-producing areas has become more and more serious year by year, and conventional chemical agents are difficult to prevent and control, which often leads to an increase in the prevalence of the disease. Summary of the invention

[0006] The purpose of the present invention is to provide a pesticide composition for preventing and treating ginger root rot and application thereof, so as to solve the problems existing in the above-mentioned prior art.

[0007] One of the technical solutions provided by the present invention:

[0008] A pesticide composition for preventing and treating ginger root rot comprises meconium-methyl, metalaxyl-M and ethopropinol.

[0009] Preferably, the mass ratio of benomyl, metalaxyl-M and ethopropin is 2:1:3.

[0010] Metalaxyl-M, chemical name N-(2,6-xylyl)-N-(methoxyacetyl)-D-alanine methyl ester, is a broad-spectrum fungicide, mainly used to prevent and treat plant diseases caused by pathogens of the Oomycetes family, and has significant effects on diseases of vegetables, fruit trees, grains and other crops caused by downy mildew, phytophthora and pythium. Hymexazol, chemical name 3-hydroxy-5-methylisoxazole, is a systemic high-efficiency pesticide fungicide, soil disinfectant, and also a plant growth regulator. It has unique efficacy, high efficiency, low toxicity, and is pollution-free. It is a green and environmentally friendly high-tech product. Hymexazol can effectively inhibit the normal growth of pathogenic fungal mycelium or directly kill pathogens, and can also promote plant growth. It also promotes the growth and development of crop roots, rooting and seedlings, and improves the survival rate of crops. Ethylicin, also known as ethyl thiosulfonate in Chinese, is a plant bionic pesticide that can effectively prevent and treat a variety of diseases such as fungi and bacteria. Its bactericidal mechanism is that the (SS=O=O) group in its molecular structure reacts with substances containing -SH groups in bacterial molecules, thereby inhibiting the normal metabolism of bacteria. The present invention has carried out a screening test from a number of pharmaceutical compounds, and found that these three pharmaceutical compounds are compounded in a certain ratio and used, and there is a good synergistic effect on the prevention and control of ginger root rot, which overcomes the defect that the prevention and control effect of ginger root rot is significantly insufficient when used alone.

[0011] The second technical solution provided by the present invention is:

[0012] An application of the above pesticide composition for preventing and treating ginger root rot is to disinfect the soil with a compound fungicide containing the above pesticide composition before sowing ginger seeds.

[0013] Preferably, the dosage is 7.5-9 g / mu, diluted with water, and then sprayed on the soil with a sprayer. More preferably, the dosage is 9 g / mu, diluted with water, and then sprayed on the soil with a sprayer.

[0014] The third technical solution provided by the present invention is:

[0015] An application of the above pesticide composition for preventing and treating ginger root rot, wherein the ginger seeds are soaked and disinfected with a compound fungicide containing the above pesticide composition before germination.

[0016] Before germinating ginger seeds, they should be soaked and disinfected with a compound fungicide to prevent the seeds from being infected with bacteria.

[0017] Preferably, every 100 kg of seeds are soaked in an aqueous solution containing 7.5-9 g of the pesticide composition, and more preferably, every 100 kg of seeds are soaked in an aqueous solution containing 7.5 g of the pesticide composition.

[0018] Preferably, the compound fungicide comprises the above-mentioned pesticide composition and pharmaceutical excipients.

[0019] Preferably, the compound fungicide includes a water-based preparation and a solid preparation.

[0020] More preferably, the water-based preparation includes one or more of emulsifiable concentrates, suspensions, aqueous emulsions and microemulsions.

[0021] More preferably, the solid preparation comprises one or more of granules, powders and dispersible tablets.

[0022] Compared with the prior art, the present invention has the following advantages and technical effects:

[0023] The invention provides a pesticide composition for preventing and treating ginger root rot. The pesticide composition comprises meconium-methyl, metalaxyl-M and ethiocarb. The composition is used to prepare a compound fungicide, wherein the suspending agent can effectively prevent and treat ginger root rot. DETAILED DESCRIPTION

[0024] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0025] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0026] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0027] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0028] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0029] The test strains of ginger root rot pathogens in the embodiments of the present invention include: Fusarium oxysporum, Fusarium solani and Pythium glomerulosa, which are provided by the laboratory of Tangshan Academy of Agricultural Sciences. Typical root rot diseased ginger samples were collected from ginger planting areas in Fengrun District, Tangshan City, Hebei Province from 2021 to 2022, and separated, purified and identified by tissue separation, morphological and molecular biological methods, and verified by Koch's postulates.

[0030] The technical materials of the fungicides tested in the examples of the present invention were all purchased from the market.

[0031] Example 1

[0032] In this example, the mycelium growth rate method was used to determine the toxicity of Fusarium oxysporum, Fusarium solani and Pythium glomerulosa with different fungicides to screen for agents with good antibacterial effects.

[0033] The original drugs of fungicides azoxystrobin, fludioxonil, difenoconazole, meprobamate, metalaxyl-M, ethopropinol, copper pyrifos and chlorobromoisocyanuric acid were dissolved in a small amount of ethanol or acetone solvent, and then prepared into mother solutions with a mass concentration of 10000 mg / L with sterile water. According to the preliminary experiment, drug-containing PAD culture medium with different drug series concentration gradients was prepared. Under sterile conditions, a puncher was used to punch a 5mm diameter bacterial cake along the edge of the colony of the activated cultured test strain, and the mycelium was inoculated with the center of the above-mentioned drug-containing plate with the mycelium facing down. Each treatment was repeated 3 times, and a blank control was set. After culturing at 28℃ for 5-7 days, the colony diameter was measured, the average inhibition rate of mycelium growth was calculated, and a regression equation was established to calculate the EC 50 , where mycelium growth inhibition rate = (control colony diameter - treated cake diameter) / (control colony diameter - 5 mm) × 100%, the measurement results are shown in Tables 1 to 3.

[0034] Table 1 Indoor toxicity test of 8 fungicides against Fusarium oxysporum

[0035]

[0036] As shown in Table 1, among the eight fungicides, the three fungicides with the best antibacterial effects on Fusarium oxysporum are ethiocarb, fludioxonil, and meconium chloride.50 The values ​​from small to large are ethoxypropynol (11.49 mg / L), fludioxonil (17.40 mg / L), and meconium chloride (33.09 mg / L).

[0037] Table 2 Indoor toxicity test of 8 fungicides against Fusarium solani

[0038]

[0039] As shown in Table 2, among the eight fungicides, the best inhibitory effects on Fusarium solani were also found in ethoxylated, fludioxonil, and meconium chloride, with EC values ​​of 50 The values ​​from small to large are ethoxypropynol (10.66 mg / L), fludioxonil (15.71 mg / L), and meconium chloride (28.56 mg / L).

[0040] Table 3 Indoor toxicity test of 8 fungicides against Pythium glomerata

[0041]

[0042] As shown in Table 3, among the eight fungicides, the three fungicides with the best inhibitory effects on Pythium spp. are metalaxyl, ethoxychlor, and oxazolidinone. 50 The values ​​from small to large are: metalaxyl-M (2.57 mg / L), ethopropinol (9.70 mg / L), and meconium-methyl (15.56 mg / L).

[0043] According to the indoor toxicity test of 8 fungicides, ethidone has the best inhibitory effect on Fusarium, and also has a good inhibitory effect on Pythium. Metalaxyl-M has the best inhibitory effect on Pythium, EC 50 The value was only 2.57 mg / L; carbendazim had good antibacterial effect on the three pathogens, and its antibacterial effect ranked third. After comprehensive consideration, we finally chose to compound the above three agents and screen the compound combination.

[0044] Example 2

[0045] The mother solutions of three drugs (10000 mg / L) of oxadipate, metalaxyl-M and ethiocarb were mixed in different mass ratios (1:1:1, 3:1:2, 3:2:1, 2:1:3, 2:3:1, 1:3:2, 1:2:3, 4:1:1, 1:4:1, 1:1:4) to obtain mixed solutions of different proportions, which were then diluted with sterile water to obtain drug solutions with mass concentrations of 1000, 100, 10, 1, and 0.1 mg / L, and respectively mixed with PDA culture medium in a volume ratio of 1:9 to prepare PDA plates with drug contents of 100, 10, 1, 0.1, and 0.01 mg / L. The toxicity was determined by the mycelial growth rate inhibition method, which was the same as in Example 1.

[0046] The synergistic coefficient (SR) was calculated according to the Wadley formula.

[0047] EC 50 (th) = (a + b) / [a / EC 50 (A)+b / EC 50 (B)] (1)

[0048] SR=EC 50 (th) / EC 50 (ob) (2)

[0049] In the formula, A and B are single agents, a and b are the proportions of the corresponding single agents in the mixture, ob is the actual observed value, and th is the theoretical value. SR>1.5 is a synergistic effect; 0.5≤SR≤1.5 is an additive effect; SR<0.5 is an antagonistic effect. The test results are shown in Table 4-6.

[0050] Table 4 Combined toxicity test of metalaxyl, methylsulfoxam and ethylsulfoxam against Fusarium oxysporum

[0051]

[0052]

[0053] The test results in Table 4 show that among the 10 compound ratios of mexamethylenetetramine, metalaxyl-M and ethoprop, 2 ratios have synergistic effects on the inhibition of Fusarium oxysporum. Among them, when the mass ratio of mexamethylenetetramine, metalaxyl-M and ethoprop is 2:1:3, the synergistic coefficient is the highest and the synergistic effect is the most obvious.

[0054] Table 5 Combined toxicity test of metalaxyl, methyl and ethoxylated against Fusarium solani

[0055]

[0056]

[0057] The test results in Table 5 show that among the 10 compound ratios of mexamethylenetetramine, metalaxyl-M and ethoprop, one ratio has a synergistic effect on the inhibition of Fusarium solani, that is, the mass ratio of mexamethylenetetramine, metalaxyl-M and ethoprop is 2:1:3, and the synergistic effect is most obvious.

[0058] Table 6 Combined toxicity test of metalaxyl, methylsulfonyl and ethylsulfonyl against Pythium spp.

[0059]

[0060]

[0061] The test results in Table 6 show that among the 10 compound ratios of mexamethylenetetramine, metalaxyl-M and ethoprop, 5 ratios have synergistic effects on the inhibition of Pythium spp. When the mass ratio of mexamethylenetetramine, metalaxyl-M and ethoprop is 2:1:3, the synergistic coefficient is the highest and the synergistic effect is the most obvious.

[0062] In summary, among all the compound agents screened, the quality ratio with the best compound effect is meconium-methyl:metalaxyl-M:ethionyl=2:1:3.

[0063] Example 3

[0064] The pesticide active ingredients (metalaxyl-M, benomyl and ethopropion), various adjuvants (Morwet EFW, polyvinyl alcohol, emulsifying dispersant 2280, xanthan gum and sodium benzoate) and water were uniformly mixed and stirred for 30 minutes, and then crushed with a sand mill to a multiphase suspension system with a particle size of ≤5 μm to prepare a suspension concentrate, in which the active ingredient content was 300 g / L. The content of each component in the suspension concentrate is shown in Table 7.

[0065] Table 7

[0066]

[0067]

[0068] Note: g / L indicates the mass of active ingredients or adjuvants contained in each liter of suspension concentrate.

[0069] Example 4

[0070] Potted plant efficacy test: The test site is the ginger laboratory of Tangshan Academy of Agricultural Sciences, and the test time is June-September 2023. Ginger harvested from the plot with the most serious root rot in the previous year is used as ginger seeds. Before germination, the ginger seeds are respectively prepared with the suspension prepared in Example 3 to prepare different concentrations of liquid medicine for soaking and disinfection of ginger seeds for 3 minutes.

[0071] The control groups were treated with three single agents of 350g / L metalaxyl seed treatment emulsion (available commercially), 30% metalaxyl aqueous solution (available commercially) and 80% ethoxylated emulsion (available commercially), and the blank control group was treated with clean water. The dosage of the agent is shown in Table 8. After soaking, the ginger seeds were placed in a 28°C incubator for germination. When the buds grew to about 1.5cm, they were sown in a seedling pot filled with sterile soil. 30 plants were sown for each treatment, and each group was repeated 3 times. After the seedlings emerged, the emergence rate was counted, and the incidence and disease index of root rot were counted after 60 days, and the prevention effect of the agent was calculated. The test results are shown in Table 8.

[0072] Among them, germination rate (%) = number of germination / number of sowing × 100%;

[0073] Disease index = 100 × ∑ (number of diseased plants at each level × relative level value) / (total number of plants surveyed × representative value of the most severe disease level);

[0074] Control effect (%) = (disease index of blank control area - disease index of drug-treated area) / disease index of blank control area × 100%.

[0075] Table 8 Effect of the suspension prepared in Example 3 on the ginger germination rate and root rot control in potted test

[0076]

[0077]

[0078] As shown in Table 8, the effects of suspension agent A, B, C, and D on ginger root rot were 71.74%, 79.24%, 80.92%, and 82.66%, respectively. The effects of metalaxyl, benomyl, and ethoxypropyrin on ginger root rot were 60.16%, 65.92%, and 72.77%, respectively. The dosage of the active ingredient of the suspension agent was 7.5-10.5 g / 100 kg seeds, and the effect on ginger root rot was significantly higher than that of the single agent treatment. However, with the increase of the drug concentration, the ginger emergence rate would be affected to a certain extent. When the dosage of the active ingredient of the suspension agent was 7.5-9 g / 100 kg seeds, the ginger seeds were disinfected, which had no significant effect on the ginger emergence rate, and the effect on ginger root rot was significantly higher than that of the three single agent treatments. Among them, the effect of the suspension agent was the best when the active ingredient was 7.5 g / 100 kg seeds.

[0079] Example 5

[0080] Field efficacy test: The test site is Shanwangzhai Village, Fengrun District, Tangshan City, Hebei Province. The test field had moderate root rot last year, with an incidence rate of 20%. The test area is 300m 2 After the ginger seeds were soaked and disinfected with the suspension prepared in Example 3 and germinated, they were sown in the experimental field on March 31, 2024 and cultivated in a plastic arch shed with a plot area of ​​12.5 m 2 , repeated 3 times. Before sowing, the experimental field was deeply plowed and rotary tilled to mix the soil, and then the soil was disinfected with pesticides. The pesticides and dosages of each treatment are shown in Table 9. The emergence rate was counted 30 days after emergence, and the occurrence of root rot was counted 60 days later, and the disease index and prevention effect were calculated.

[0081] Table 9 The control effect of the suspension prepared in Example 3 on the ginger germination rate and root rot in the field test

[0082]

[0083]

[0084] The results of the field test are shown in Table 9: The control effects of suspension A, B, and C on ginger root rot were 63.6%, 71.35%, and 74.08%, respectively, and the control effects of metalaxyl-M and oxadixyl-Med E and F on ginger root rot were 49.08% and 53.25%, respectively. The treatments of suspension D and 80% ethoxylated emulsion G significantly affected the ginger emergence rate, so the control effect statistics were not made. The effective ingredient dosage of the suspension was 7.5-9g / mu, which had no effect on the ginger emergence rate, and the control effect on ginger root rot was significantly higher than that of metalaxyl-Med and oxadixyl-Med single-agent treatments. Among them, the effective ingredient dosage of the suspension was 9g / mu, which had the best effect on the soil.

[0085] The above are only preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A pesticide composition for preventing and treating ginger root rot, characterized in that: Including metalaxyl, methylsulfoxide and ethidium bromide.

2. The pesticide composition for preventing and treating ginger root rot according to claim 1, characterized in that: The mass ratio of the metalaxyl-M to ethoxycarbonyl is 2:1:

3.

3. Use of the pesticide composition for preventing and treating ginger root rot according to claim 1 or 2, characterized in that: The soil is disinfected with a compound fungicide containing the pesticide composition according to claim 1 or 2 before ginger seeds are sown.

4. Use of the pesticide composition for preventing and treating ginger root rot according to claim 1 or 2, characterized in that: Before germination of ginger seeds, a compound fungicide containing the pesticide composition according to claim 1 or 2 is used to soak and disinfect the ginger seeds.

5. The use according to claim 3 or 4, characterized in that: The compound fungicide comprises the pesticide composition according to claim 1 or 2 and pharmaceutical excipients.

6. The use according to claim 5, characterized in that: The compound fungicide includes a water-based preparation and a solid preparation.

7. The use according to claim 6, characterized in that: The water-based preparation includes one or more of emulsifiable concentrates, suspensions, aqueous emulsions and microemulsions.

8. The use according to claim 6, characterized in that: The solid preparation includes one or more of granules, powders and dispersible tablets.

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