RNA (Ribonucleic Acid) and DNA (Deoxyribose Nucleic Acid) dual-fluorescence activated cell sorting technique and application thereof in cell subtype typing and cell genetic material research
Through dual fluorescence activated cell sorting of RNA and DNA, double staining and flow cytometry analysis of testicular cells was performed, precise typing and genetic material research on cell subtypes was achieved, and the problem of insufficient DNA and RNA typing ability of testicular cells in the prior art was solved, and the research method was provided on the dynamic changes in germ cell RNA abundance and the function of RNA binding proteins was provided.
CN119804276APending Publication Date: 2025-04-11FOURTH MILITARY MEDICAL UNIVERSITY
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Patent Information
- Application Number
- CN202510015919.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-06
- Publication Date
- 2025-04-11
AI Technical Summary
Technical Problem
The prior art is difficult to achieve precise subtyping of DNA and RNA in testicular cells, and there is a lack of effective methods to study the dynamic changes of cellular genetic material.
Method used
The dual fluorescence activated cell sorting of RNA and DNA was used to double stain the cells with DNA and RNA, and fluorescence activated cell sorting by flow cytometry, so as to achieve accurate typing and genetic material research on different cell subtypes.
Benefits of technology
The precise classification of different cell subtypes in testicular cells, especially the dynamic changes in RNA abundance of germ cells, has been achieved, providing a means of research on the function of RNA binding proteins, and providing an effective method for the study of spermgenesis.
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Abstract
The invention provides an RNA (Ribonucleic Acid) and DNA (Deoxyribose Nucleic Acid) dual-fluorescence activated cell sorting technique and application thereof in cell subtype typing and cell genetic material research, and belongs to the technical field of molecular biology, the RNA and DNA dual-fluorescence activated cell sorting technique comprises the following steps: 1) digesting cell tissues to obtain a single-cell suspension; 2) carrying out double staining of DNA and RNA on the digested single-cell suspension; and (3) carrying out flow cytometry analysis and fluorescence activation cell sorting on the dyed cells. According to the method, RNA and DNA in the cells are subjected to double staining, so that cell types which are difficult to distinguish in the past can be distinguished more accurately. The invention provides a Ramp; the D-FACS provides an effective means for further fine research on the spermatogenesis process, and provides an effective method for research on sperm abnormality and sterility.
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