Immunofluorescence detection reagent for HCR signal amplification
By using HCR signal amplification method in immunofluorescence detection technology, the fluorescent signal is amplified by linear hybrid chain reaction, which solves the problem of low detection sensitivity in the prior art, and achieves high sensitivity protein and small molecule detection.
Patent Information
- Application Number
- CN202311344436.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-17
- Publication Date
- 2025-04-18
AI Technical Summary
The existing immunofluorescence detection technology has problems such as non-specific staining, insufficient objectivity of the result judgment, complex technical procedures, and low fluorescence performance, making it difficult to achieve high sensitivity quantitative detection.
Immunofluorescence detection reagent with HCR signal amplification is used to form a double-stranded DNA product carrying a large amount of biotin through linear hybrid chain reaction, which is linked to the fluorescent dye-labeled streptavidin, amplifying the constant fluorescence signal and improving detection sensitivity.
It greatly improves detection sensitivity, achieves high sensitivity detection of disease markers such as proteins and small molecules, and improves the sensitivity and stability of immunofluorescence technology.