A detection primer and kit for Vibrio parahaemolyticus and its application

By developing a SYBR Green I RT-PCR method using specific primer sets for the vhvp-1, vhvp-2, and vhvp-3 genes, the problem of rapid detection of Vibrio parahaemolyticus in whiteleg shrimp farming has been solved, achieving efficient and economical pathogen monitoring and supporting early warning and prevention of diseases in shrimp farming.

CN120174126BActive Publication Date: 2025-09-23SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA
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Patent Information

Application Number
CN202510655425.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-21
Publication Date
2025-09-23
Estimated Expiration
2045-05-21

AI Technical Summary

Technical Problem

Existing technologies make it difficult to quickly, conveniently, sensitively and economically detect Vibrio parahaemolyticus in whiteleg shrimp farming, resulting in difficulties in diagnosing vitreous seedling disease and affecting farming production efficiency.

Method used

A highly specific and sensitive SYBR Green I RT-PCR detection method was developed, using a primer set specific for the vhvp-1, vhvp-2, and vhvp-3 genes, including upstream and downstream primers, combined with fluorescent dyes and control samples to establish a rapid detection system.

Benefits of technology

It has achieved efficient, economical and convenient detection of Vibrio parahaemolyticus in the tissues of white shrimp, supported early warning and precise prevention and control of diseases, and promoted the sustainable development of the shrimp farming industry.

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Abstract

The present invention discloses a detection primer, a kit and its application for Vibrio parahaemolyticus, comprising an upstream primer F and a downstream primer R; the upstream primer R is selected from one or more of SEQ ID NO.10, SEQ ID NO.26 and SEQ ID NO.40, and the downstream primer R is selected from one or more of SEQ ID NO.11, SEQ ID NO.27 and SEQ ID NO.41. The primer set can be used in the detection of Vibrio parahaemolyticus and the preparation of Vibrio parahaemolyticus detection preparations and kits for non-diagnostic purposes. The present application establishes a standardized VpTPD pathogen detection method and develops a rapid, convenient, highly sensitive, highly specific, economical and practical detection primer and kit that can be widely promoted for pathogen detection, thereby solving the effective prevention and efficient monitoring of Vibrio parahaemolyticus.
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