Condensed ring polyphenol structure and application thereof

Abstract

The invention discloses a condensed ring polyphenol structure and application thereof, and relates to the technical field of organic chemistry. The condensed ring polyphenol is formed by connecting one or more cluster structures, each cluster structure is composed of a parent nucleus structure formed by a carbon ring or a carbon heterocyclic ring and an oxygen-containing functional group covalently connected to the ring of the parent nucleus structure, and the oxygen-containing functional group is a hydroxyl group, a carboxyl group, a hydroxyl group or a carboxyl group. The structural formula is selected from any one or more of carbonyl, epoxy group, ester group, ether bond, nitryl, alkoxy, acylamino and sulfonyl. The use mode, the use object, the use stage, the product form and the like of the polycyclic polyphenols are not limited, when the polycyclic polyphenols directly and / or indirectly act on crops, a nutrition transportation channel of the crops is opened to be used for transporting specific nutrient elements, the growth of the crops can be promoted, and the yield increasing effect is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of organic chemistry, and particularly relates to a polycyclic polyphenol structure and its application. Background Art

[0002] Polycyclic polyphenol is a two-dimensional structured hydrated nano-solution based on the SP2 hybridization of carbon atoms, presenting a honeycomb lattice of multiple hexagonal arrangements. Compared with traditional humic acid molecules (similar in partial structural appearance), this molecule removes the original sugar and amino acid residues and other parts that are easily degraded in the environment, and replaces them with a polycyclic polyphenol skeleton with good biological activity as the main body. Coupled with its own hydrophobic (SP2) two-dimensional sheet structure, hydrophilic (SP3) oxygen-containing functional groups (-OH, -COOH, --CH(O)CH-), high specific surface area effect, and monolayer small size effect, the structure has excellent dispersibility in aqueous solution.

[0003] The environmental conditions affecting the growth and development of crops mainly include light, temperature, water, air, fertilizers, etc. Only under suitable environmental conditions can crops grow and develop normally and achieve high quality and high yield. Crops cannot survive without light and all require appropriate light for photosynthesis to provide an environment for the metabolism and survival of crops. Crops need to survive in a certain temperature environment, and water is the most important component for the survival of crops. The cell metabolism of crops is also completed through water, and metabolism needs to be completed through transpiration and respiration. Water plays an important role in crop moisture retention, nutrient solution transportation, and substrate soil. Crop metabolism also requires air circulation. In an environment with poor ventilation, crops are prone to grow poorly and even suffer from leaf blight, leaf rot, pests, etc. Fertilizer in a broad sense can also include agents such as bactericides, insecticides, nutrients, and cleaners. The demand of crops for agents also requires scientific use.

[0004] However, currently, during the growth process of crops, due to the influence of extensive use of chemical fertilizers in the soil, the soil will show compaction, salinization, and acidification, resulting in serious loss of nutrients in the soil and poor leaching effect, directly affecting the growth of crops. Moreover, during the planting process, there will also be the influence of pests and diseases on the crops, further reducing the yield of crops.

[0005] Therefore, providing a polycyclic polyphenol structure that can increase the yield of crops is an urgent technical problem to be solved by the present invention. Summary of the Invention

[0006] A polycyclic polyphenol structure and its application provided by the present invention aim to solve the problems existing in the above background art.

[0007] In order to achieve the above technical objectives, the present invention mainly adopts the following technical solutions:

[0008] In a first aspect, the present invention discloses a polycyclic polyphenol structure, which is composed of one or more cluster structures connected together. The cluster structure is composed of a parent nucleus structure formed by a carbocyclic ring or a heterocyclic ring and oxygen-containing functional groups covalently connected to the ring of the parent nucleus structure. The oxygen-containing functional groups are any one or more of hydroxyl, carboxyl, carbonyl, epoxy group, ester group, ether bond, nitro group, alkoxy group, amide group, and sulfonyl group.

[0009] In a preferred embodiment of the present invention, the hetero elements in the heterocyclic ring include at least one of oxygen, nitrogen, phosphorus, and sulfur elements.

[0010] Preferably, the number of rings of the carbocyclic ring or the heterocyclic ring is 3 - 12.

[0011] Furthermore, when the parent nucleus structure is a three-membered ring, the carbocyclic ring or the heterocyclic ring includes a three-membered carbocyclic ring containing only carbon elements, or a three-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The three-membered carbon heterocyclic ring includes a three-membered carbon heterocyclic ring composed only of single bonds or a three-membered carbon heterocyclic ring containing one double bond;

[0012] When the parent nucleus structure is a four-membered ring, the carbocyclic ring or the heterocyclic ring includes a four-membered carbocyclic ring containing only carbon elements, or a four-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The four-membered carbon heterocyclic ring includes a four-membered carbon heterocyclic ring composed only of single bonds or a four-membered carbon heterocyclic ring containing one or two double bonds;

[0013] When the parent nucleus structure is a five-membered ring, the carbocyclic ring or the heterocyclic ring includes a five-membered carbocyclic ring containing only carbon elements, or a five-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The five-membered carbon heterocyclic ring includes a five-membered carbon heterocyclic ring composed only of single bonds or a five-membered carbon heterocyclic ring containing one or two double bonds;

[0014] When the parent nucleus structure is a six-membered ring, the carbocyclic ring or the heterocyclic ring includes a six-membered carbocyclic ring containing only carbon elements, or a six-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The six-membered carbon heterocyclic ring includes a six-membered carbon heterocyclic ring composed only of single bonds or a six-membered carbon heterocyclic ring containing one or two or three double bonds;

[0015] When the parent nucleus structure is a seven-membered ring, the carbocyclic ring or the heterocyclic ring includes a seven-membered carbocyclic ring containing only carbon elements, or a seven-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The seven-membered carbon heterocyclic ring includes a seven-membered carbon heterocyclic ring composed only of single bonds or a seven-membered carbon heterocyclic ring containing one or two or three double bonds;

[0016] When the parent nucleus structure is an eight-membered ring, the carbocyclic or heterocyclic ring includes an eight-membered carbocyclic ring containing only carbon elements, or an eight-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The eight-membered carbon heterocyclic ring includes an eight-membered carbon heterocyclic ring composed only of single bonds or an eight-membered carbon heterocyclic ring containing one or more double bonds;

[0017] When the parent nucleus structure is a nine-membered ring, the carbocyclic or heterocyclic ring includes a nine-membered carbocyclic ring containing only carbon elements, or a nine-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The nine-membered carbon heterocyclic ring includes a nine-membered carbon heterocyclic ring composed only of single bonds or a nine-membered carbon heterocyclic ring containing one or more double bonds;

[0018] When the parent nucleus structure is a ten-membered ring, the carbocyclic or heterocyclic ring includes a ten-membered carbocyclic ring containing only carbon elements, or a ten-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The ten-membered carbon heterocyclic ring includes a ten-membered carbon heterocyclic ring composed only of single bonds or a ten-membered carbon heterocyclic ring containing one or more double bonds;

[0019] When the parent nucleus structure is an eleven-membered ring, the carbocyclic or heterocyclic ring includes an eleven-membered carbocyclic ring containing only carbon elements, or an eleven-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The eleven-membered carbon heterocyclic ring includes an eleven-membered carbon heterocyclic ring composed only of single bonds or an eleven-membered carbon heterocyclic ring containing one or more double bonds;

[0020] When the parent nucleus structure is a twelve-membered ring, the carbocyclic or heterocyclic ring includes a twelve-membered carbocyclic ring containing only carbon elements, or a twelve-membered carbon heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The twelve-membered carbon heterocyclic ring includes a twelve-membered carbon heterocyclic ring composed only of single bonds or a twelve-membered carbon heterocyclic ring containing one or more double bonds.

[0021] Furthermore, when the parent nucleus structure is a three-membered ring, the parent nucleus structure is selected from any one of Cyclopropane or cyclopropene ; when the parent nucleus structure is a four-membered ring, the parent nucleus structure is selected from any one of cyclobutane, cyclobutene, or 1,3-cyclobutadiene; when the parent nucleus structure is a five-membered ring, the parent nucleus structure is selected from any one of cyclopentane, cyclopentene, 1,3-cyclopentadiene, furan, thiophene, or pyrrole; when the parent nucleus structure is a six-membered ring, the parent nucleus structure is selected from any one of cyclohexane, cyclohexene, 1,3-cyclohexadiene, 1,4-cyclohexadiene, benzene ring, pyran, pyridine, or pyrimidine; when the parent nucleus structure is a seven-membered ring, the parent nucleus structure is selected from any one of cycloheptane, cycloheptene, 1,3-cycloheptadiene, 1,3,5-cycloheptatriene, oxepane, or 1H-azepine; when the parent nucleus structure is an 8- to 12-membered ring, the parent nucleus structure is selected from a saturated carbocyclic or heterocyclic ring or an unsaturated carbocyclic or heterocyclic ring.

[0022] In a preferred embodiment of the present invention, the cluster structure is one or more.

[0023] Furthermore, when there are multiple cluster structures, the connection modes between carbon rings or carbon-heterocyclic rings in the cluster structures include bond connection, fused-ring connection, and spiro-ring connection.

[0024] Even further, the bond connection between rings includes bond connections composed of the same element among carbon, oxygen, nitrogen, phosphorus, and sulfur and bond connections composed of different elements; the fused-ring connection between rings includes one fused-ring mode between two rings, two fused-ring modes between three rings, four fused-ring modes between four rings, and when there are more than three rings, the fused-ring mode along the direction that makes the ring structures in a row or the direction perpendicular thereto.

[0025] In a preferred embodiment of the present invention, the average carbon-oxygen ratio of the polycyclic polyphenol is 1.1 - 2.0.

[0026] In a second aspect, the present invention discloses an application of the polycyclic polyphenol structure as described in the first aspect in agricultural production.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The polycyclic polyphenol provided by the present invention has a wide range of usage methods. When the polycyclic polyphenol acts directly and / or indirectly on crops, the polycyclic polyphenol can open the nutrient transport channels of the crops to transport specific nutrient elements, including metal ions and non-metal ions, to supplement nutrients for the crops. When the polycyclic polyphenol acts indirectly on crops, the polycyclic polyphenol is compounded with nutrient elements to transport the specific nutrient elements to the target locations of the crops, and the nutrient elements include macronutrients and micronutrients. By using the polycyclic polyphenol in various ways, the absorption rate of specific nutrient elements by the organisms during the growth cycle can be increased, which can promote the growth of crops and has the effect of increasing yields. Description of the Drawings

[0029] Figure 1 It is the infrared spectrum detection diagram of the polycyclic polyphenol prepared in Example 1 of the present invention;

[0030] Figure 2 It is the prevention and control effect diagram of the polycyclic polyphenol on peanut soil-borne diseases provided by the experimental examples of the present invention;

[0031] Figure 3 It is the diagram of the influence of different treatments of the polycyclic polyphenol on the yield of Tremella fuciformis provided by the experimental examples of the present invention;

[0032] Figure 4 It is the comparison diagram of the rose diameter and rose cut flower life data results after treatment of each group provided by the experimental examples of the present invention;

[0033] Figure 5A comparison chart of the RFWs (relative fresh cut flower weight) data results of the processed rose cut flowers in each group provided for the experimental examples of the present invention. Detailed implementation manners

[0034] To make the objectives, technical solutions and advantages of the present application clearer, the following will further describe in detail the embodiments of the present application with reference to the accompanying drawings.

[0035] When the following description refers to the accompanying drawings, unless otherwise indicated, the same numbers in different drawings represent the same or similar elements. The implementation manners described in the following exemplary embodiments do not represent all the implementation manners consistent with the present application. On the contrary, they are merely examples of the devices and methods consistent with some aspects of the present application as detailed in the appended claims.

[0036] The present invention provides a polycyclic polyphenol structure, without limiting the specific usage mode, nor the target crops, such as the widely distributed cruciferous plants. The cruciferous plants are a widely distributed plant family. There are many varieties in the cruciferous family that can be eaten by humans or used as oil crops, such as Chinese cabbage, radish, mustard, rapeseed, cabbage, etc.; among them, there are also many varieties that are beneficial to human health, such as Isatis indigotica, violet, etc., which have both medicinal and ornamental values. During the cultivation process of cruciferous plants, the cruciferous plants will go through stages from seeds to germination, then to growing into adult plants, flowering and then fruiting. At different stages of cruciferous plants, it is necessary to supplement the essential nutrient elements for their growth and development. These nutrient elements include, but are not limited to, potassium, calcium, magnesium, iron, zinc, copper, carbon, hydrogen, oxygen, nitrogen, phosphorus, sulfur, etc. These nutrient elements enter the plant body through the respiratory chain and / or photosynthetic chain of the plant and are utilized by the plant.

[0037] The polycyclic polyphenol acts directly and / or indirectly on the crops to increase the absorption rate of the specific nutrient elements by the organism during the growth cycle. Specifically, it opens the nutrient transport channels for transporting the specific nutrient elements to supplement the nutrient for the organism. For example, when transporting nutrient elements such as potassium, calcium, magnesium, iron, zinc, copper, carbon, hydrogen, oxygen, nitrogen, phosphorus, sulfur, etc., the channels will only be opened under specific circumstances, such as when affected by stress or adverse environment or when the plant is flowering and fruiting. Then the plant absorbs the nutrient elements. The absorption of nutrient elements by the plant is affected by specific circumstances and the channels cannot be opened at any time to absorb nutrient elements; while the present invention utilizes the characteristic that the polycyclic polyphenol can open the nutrient transport channels of the organism, so that the organism is no longer restricted by specific circumstances. As long as the organism comes into contact with the polycyclic polyphenol, it can open the nutrient transport channels to absorb nutrient elements, and can supplement the nutrient elements for the organism at all times, making the organism obtain nutrients more fully.

[0038] For the specific form, polycyclic polyphenols can be sold separately as a compounding material, that is, a separately sold compounding ingredient. When used subsequently, it can be added to the product for use; it can also be sold as a new compounding substance after compounding polycyclic polyphenols with the product. For example, polycyclic polyphenols can be added to fertilizers as a new type of fertilizer, or polycyclic polyphenols can be added to preservatives as a separate product.

[0039] For the specific usage methods, they include: directly diluting and irrigating polycyclic polyphenols, mixing polycyclic polyphenol solutions with substrate soil, mixing polycyclic polyphenol solutions with nutrient soil, mixing polycyclic polyphenols with fertilizers, diluting and mixing polycyclic polyphenols with special fertilizers (water-soluble fertilizers), compounding polycyclic polyphenols with copper preparations, mixing polycyclic polyphenols with preservatives, spraying and injecting polycyclic polyphenol solutions, and so on.

[0040] The time period when polycyclic polyphenols come into contact with crops can be applicable to various different growth stages, including the seed stage, seedling stage, adult plant stage, flower stage, fruiting stage, etc. For the contact parts, in the seed stage, polycyclic polyphenols can act on the embryo, radicle, hypocotyl, and cotyledons; in the seedling stage, polycyclic polyphenols can act on the roots, stems, and leaves; in the adult plant stage, polycyclic polyphenols can act on the roots, stems, and leaves; in the flower stage, polycyclic polyphenols can act on the roots, stems, leaves, and flowers; in the fruiting stage, polycyclic polyphenols can act on the roots, stems, leaves, and fruits.

[0041] The polycyclic polyphenols of the present invention include a parent nucleus structure formed by a carbocyclic or heterocyclic ring and oxygen-containing functional groups covalently connected to the rings of the parent nucleus structure, which is beneficial to promoting the opening of nutrient transport channels to supplement nutrients.

[0042] Taking K + as an example, the oxygen-containing functional groups can be connected to potassium ions in the soil or other products through oxidative coordination, equivalent to carrying potassium ions. And polycyclic polyphenols themselves have strong water solubility and can carry a large amount of potassium ions into the cells of the plant body along with water molecules; moreover, multiple oxygen-containing functional groups can be simultaneously present on polycyclic polyphenols, which can be simultaneously compounded with multiple potassium ions, increasing the amount of potassium ions that can be carried. The number of transported potassium ions is no longer limited by the potassium ion concentration in water molecules, further improving the utilization rate of potassium ions in the soil or other products by plants; because potassium ions directly affect the photosynthesis of plants, in this embodiment, by increasing the absorption of potassium ions by plants through polycyclic polyphenols, the photosynthesis efficiency of plants can be further improved.

[0043] When the polycyclic polyphenol includes a heterocyclic ring, there are non-carbon atoms on the heterocyclic ring, such as nitrogen, oxygen, phosphorus, sulfur, etc. The above atoms can be connected to potassium ions in the soil or other products through oxidative coordination, thereby carrying potassium ions. Based on the strong water solubility of polycyclic polyphenols, polycyclic polyphenols can carry a large amount of potassium ions into the cells of plants along with water molecules, and the amount of transported potassium ions is no longer limited by the potassium ion concentration in the water molecules, further improving the utilization rate of potassium ions in the soil or other products by plants.

[0044] In addition, oxygen-containing functional groups or heterocyclic rings can all be connected to potassium ions in the soil or other products, and can be used in various growth stages to make various products. For example, for the seed stage, polycyclic polyphenol products can be coating agents, fungicides, insecticides, seed culture media, ethylene, organic matter soil, etc.; for the seedling stage, polycyclic polyphenol products can be fertilizers, pesticides, etc.; for the adult plant stage, polycyclic polyphenol products can be plant growth regulators; for the flower stage, polycyclic polyphenol products can be preservatives, flowering regulators, flower thinning agents; for the fruiting stage, polycyclic polyphenol products can be fruit thinning agents, fruit stage regulators, defoliants.

[0045] The following is illustrated by specific examples and control examples.

[0046] Example 1 Preparation of Polycyclic Polyphenol

[0047] After washing, drying, pulverizing and sieving the carbon skeleton materials (such as straw, straw and graphite composed of cellulose and lignin, graphite is selected in this example), carbon material powder with finer particle size is obtained, which is ultrasonically dispersed in concentrated sulfuric acid to form a carbon-concentrated sulfuric acid dispersion with a mass concentration of 0.1 g / ml. After 1.5 h of ultrasonic treatment at 120 w with stirring, then, the finely ground potassium permanganate is added to concentrated phosphoric acid for dissolution, and the dissolution temperature is controlled between 40 - 60 °C. Ultrasonic stirring is carried out to make it dissolve evenly, and stearic acid is added additionally, followed by ultrasonic treatment for 0.5 h, and then natural cooling. After cooling to room temperature, the next step is carried out.

[0048] The above potassium permanganate-phosphoric acid viscous liquid is added to the carbon-concentrated sulfuric acid dispersion at a speed of 1 - 2 drops per second, and the temperature of the reaction system is controlled below 40 °C. After mixing is completed, ultrasonic stirring is carried out for 2 h. After the system becomes viscous, concentrated phosphoric acid can be added to dilute the system, and then the mixed solution is dropped into water drop by drop, with the temperature controlled below 70 °C, and the reaction is completed in 5 min. After hydrolysis is completed, the suspension in the acidic environment is ultrasonically exfoliated. After ultrasonic stirring for 2 h, it is centrifuged at a speed of 4000 r / min for 5 min to remove the unexfoliated carbon materials. The supernatant is taken as the polycyclic polyphenol colloid solution. After salting out with an equal volume of 4% ammonium sulfate solution and then filtering by suction, it is washed with water and alcohol, and then vacuum dried at 45 °C for 3 h. The obtained product is recorded as sample A.

[0049] The functional groups of the synthesized cluster-structured substance in Sample A were detected by infrared spectroscopy, and the result was ten characteristic peaks (as shown in Figure 1 ). The peak wavenumbers at the peak tops were as follows: A: 3384.88 cm-1; B: 1682.63 cm-1; C: 1618.89 cm-1; D: 1394.24 cm-1; E: 1222.11 cm-1; F: 1065.09 cm-1; G: 971.59 cm-1; H: 719.68 cm-1; I: 664.08 cm-1; J: 579.05 cm-1; K: 557.32 cm-1. After querying the characteristic peaks, it was found that Peak A characterized the aromatic C-H, Peaks B and C characterized the aromatic C═C skeleton, Peaks H and I characterized the out-of-plane bending vibration of aromatic C-H, and the broad Peak A and Peaks E and F jointly characterized that the polycyclic polyphenols with condensed rings had primary alcohol phenolic groups of carbon, hydrogen, oxygen, and hydrogen. Therefore, the infrared and elemental detection data of the substance indicated that the cluster substance was polycyclic polyphenols with an aromatic condensed ring skeleton structure, a large number of primary alcohol phenolic groups, and heteroatom functional groups, and were connected by single bonds, fused rings, or spiro rings.

[0050] Comparative Example 1

[0051] Polycyclic polyphenols were prepared by the traditional Hummers method, and the obtained product was recorded as Sample B. The corresponding characteristic peak comparison table of the infrared spectrogram of the cluster-structured substance obtained by this preparation method is shown in Table 1.

[0052]

[0053]

[0054] Comparative Example 2

[0055] Take clear water, and the obtained product is recorded as Sample C.

[0056] Test Example 1 examined the effect of the polycyclic polyphenols prepared in the examples of the present invention when used in combination with the base fertilizer

[0057] On September 10, 2019, in Chongming, Shanghai, pakchoi was sown in five plots of 0.7 m * 1 m, and 200 g of base fertilizer (15-15-15 compound fertilizer) was applied. Then, on September 25, 2019 and September 30, 2019, 10 g of Samples A, B, and C were dissolved in 100 ml of irrigation water and then used for root irrigation. After that, when any group of crops matured, they were harvested, and the harvest times are shown in Table 2 below.

[0058] Table 2 Harvest Time

[0059] Test example A B C The harvesting time is 2019-10-20 2019-10-28 2019-10-28

[0060] The results after harvesting and weighing are shown in Table 3 below.

[0061] Table 3 Weighing Results

[0062] Test example A B C Gross weight (kg) 3.3 2.6 2.5

[0063] As can be seen from Table 3, polycyclic polyphenols can result in a higher gross weight of crops compared to water and polycyclic polyphenols prepared by the traditional Hummers method, and the yield increase effect is obvious.

[0064] Another 5 plants were taken from each group for root measurement, and the results are shown in Table 4 below.

[0065] Table 4 Root measurement results

[0066]

[0067] As can be seen from Table 4, polycyclic polyphenols can promote the growth of plant roots compared to water and polycyclic polyphenols prepared by the traditional Hummers method.

[0068] Pak choi was sown on four 0.7m * 1m plots in Chongming, Shanghai on August 10, 2019, and basal fertilizer (15-15-15 compound fertilizer) was applied. Among them, the fertilizer amount for each group was 200g, 180g, 150g, and 100g, which were named the first group, the second group, the third group, and the fourth group respectively. On August 20, 2019, water was added to the first group, and the same amount of sample A as the first group was added to the second, third, and fourth groups.

[0069] The results of harvesting and weighing are shown in Table 5.

[0070] Table 5 Results of harvesting and weighing

[0071] Test example A B C D Gross weight (kg) 2.1 3.1 2.9 2.8

[0072] As can be seen from Table 5, adding the polycyclic polyphenols provided by this patent can increase the yield while saving fertilizer.

[0073] Test Example 2 examines the effect of directly using the polycyclic polyphenols prepared in the examples of the present invention

[0074] Crops grown in a conventional manner were used as the control group. The polycyclic polyphenols in this example were used as a separate product and directly applied to the crops, and were applied to different crops in different concentrations and application methods to reflect the influence of the applied polycyclic polyphenols on the crops.

[0075] In order to ensure fairness and justice during the experiment, crops with the same growth trend and planting age were planned, and areas with the same average partition size were divided into an experimental group and a control group. The polycyclic polyphenols in this example were applied to the experimental group, and the final results were averaged, and the results are as follows.

[0076] Referring to the following table, the polycyclic polyphenol solution (0.7g / L) was diluted at a ratio of 1:250 and used to water strawberries, peppers, cherry tomatoes, and purple eggplants respectively.

[0077] Refer to Chart 6. Experimental object: strawberry (fruit). Experimental field area: 2.8 m × 24 m.

[0078] Table 6 Experimental Results

[0079]

[0080] As can be seen from Table 6 above, for the strawberry fruits treated with polycyclic polyphenols, compared with those without polycyclic polyphenols, on the one hand, both the transverse diameter and the longitudinal diameter have increased, and the overall fruit size has changed significantly; on the other hand, the fruit yield has also increased, from the original 2.093 kg to 3.341 kg; on the other hand, the fruit sweetness has also increased.

[0081] Refer to Chart 7. Experimental object: pepper. Experimental field area: 1.4 m × 18 m.

[0082] Table 7 Experimental Results

[0083]

[0084] As can be seen from Table 7 above, for the peppers treated with polycyclic polyphenols, compared with those without polycyclic polyphenols, there are obvious increases in plant height, crown width, leaf length, leaf width, and stem diameter, and the yield has also increased from 50.12 kg to 60.16 kg.

[0085] Refer to Chart 8. Experimental object: cherry tomato. Experimental field area: 1.6 m × 30 m.

[0086] Table 8 Experimental Results

[0087] Experimental subject Yield Fruit cracking rate CK (control group) 141.38 kg 13.63％ Polycyclic polyphenol solution (1:250) 154.25 kg 10.9％

[0088] As can be seen from Table 8 above, for the cherry tomatoes treated with polycyclic polyphenols, compared with those without polycyclic polyphenols, there is an obvious increase in yield, and the fruit cracking rate has decreased from 13.63% to 10.9%.

[0089] Refer to Chart 9. Experimental object: purple eggplant. Experimental field area: 1.6 m × 30 m.

[0090] Table 9 Experimental Results

[0091]

[0092] As can be seen from Table 9 above, for the purple eggplants treated with polycyclic polyphenols, compared with those without polycyclic polyphenols, there is an obvious increase in yield, and the transverse diameter and longitudinal diameter of the fruits have also increased, and the number of fruit bends has also improved.

[0093] Refer to Table 10 below, dilute the polycyclic polyphenol solution (0.4 g / L) at 1:600, 1:300, and 1:150 respectively, and irrigate purple eggplants.

[0094] Table 10 Experimental Results

[0095] Experimental subject Yield (14 plants) Length Width CK (control group) 0.785 kg 14.443 cm 30.450 mm Polycyclic polyphenol solution (1:600) 1.621 kg 18.064 cm 35.858 mm Polycyclic polyphenol solution (1:300) 1.255 kg 16.935 cm 30.827 mm Polycyclic polyphenol solution (1:150) 0.697 kg 14.687 cm 33.369 mm

[0096] As can be seen from Table 10 above, for the purple eggplants treated with polycyclic polyphenols, compared with those without polycyclic polyphenols, the transverse and longitudinal diameters of the fruits of purple eggplants treated with different concentrations of polycyclic polyphenol solutions have increased. For the purple eggplants treated with polycyclic polyphenol solutions at concentrations of 1:600 and 1:300, the fruit yields have increased significantly. Moreover, the polycyclic polyphenol solution at a concentration of 1:600 is more suitable for the growth of purple eggplants. Whether in terms of the length and width of the fruits or the fruit yield, it is the optimal solution for purple eggplants.

[0097] Refer to Tables 11 and 12 below, dilute the polycyclic polyphenol solution (0.4 g / L) at 0 ppm, 70 ppm, 200 ppm, and 400 ppm (1 ppm = 0.001‰) respectively, and irrigate cucumbers and cowpeas respectively.

[0098] Table 11 Cucumber Experimental Results

[0099] Experimental subject Yield per mu Length Width CK (control group) 2912.88 kg 27.502 cm 4.161 cm 70 ppm 3501.18 kg 26.969 cm 3.843 cm 200 ppm 3933.66 kg 28.374 cm 4.012 cm 400 ppm 5075.28 kg 29.014 cm 4.015 cm

[0100] As can be seen from Table 11 above, for cucumbers treated with different concentrations of polycyclic polyphenols, compared with those without polycyclic polyphenols, there is a significant increase in yield. For cucumbers treated with polycyclic polyphenol solutions at concentrations of 200 ppm and 400 ppm, the length of the fruits is significantly longer than that of the fruits without polycyclic polyphenols. However, for cucumbers treated with different concentrations of polycyclic polyphenols, the width is smaller than that of the fruits without polycyclic polyphenols. Therefore, according to the different preferences for the width of cucumbers in different regions, cucumbers with suitable widths can be planted locally.

[0101] Table 12 Cowpea Experimental Results

[0102] Experimental subject Yield per mu Length Root length Root width CK (control group) 2673 kg 46.941 cm 4.161 cm 0.54 cm 70 ppm 2628 kg 49.271 cm 3.843 cm 0.66 cm 200 ppm 2848.5 kg 48.773 cm 4.012 cm 0.68 cm 400 ppm 3379.5 kg 50.452 cm 4.015 cm 0.84 cm

[0103] As can be seen from Table 12 above, for cowpeas treated with polycyclic polyphenol solutions at concentrations of 200 ppm and 400 ppm, compared with those without polycyclic polyphenols, there are significant increases in yield, length, and root width.

[0104] Refer to Table 13 below, dilute the polycyclic polyphenol solution at 0 ppm, 240 ppm, 667 ppm, and 1333 ppm (1 ppm = 0.001‰) respectively, and irrigate cauliflower. Experimental field area: 50 cm × 150 cm.

[0105] Table 13 Cauliflower Experiment Results

[0106]

[0107]

[0108] As can be seen from Table 13 above, for cauliflowers to which polycyclic polyphenols with concentrations of 240 ppm, 667 ppm, and 1333 ppm are applied, compared with cauliflowers without polycyclic polyphenols applied, there are obvious increases in yield. And when applying polycyclic polyphenols at a concentration of 1333 ppm, the maximum yield of cauliflowers can be achieved. The concentration of 1333 ppm is the optimal concentration for applying polycyclic polyphenols in cauliflower cultivation.

[0109] Test Example 3 Mixing the polycyclic polyphenols prepared in the examples of the present invention with substrate soil or nutrient soil

[0110] (1) Mix and compound the polycyclic polyphenol solution with substrate soil, which is loose, fine, has a large porosity, good air permeability and water retention, and a high cation exchange capacity, aiming at a light and high-quality seedling-raising substrate to ensure the cultivation of high-quality and strong seedlings. According to the usage of substrate in 96-well trays and the water demand of 1-tray seedlings, the average amount to be added at one time is 0.1 - 1.5 mL of the solution for spraying per 1 L of substrate soil, and mix evenly. A bag of substrate soil is about 20 liters, and 0.1 - 1.5 * 20 = 2 - 20 mL is required, and the required concentration is 0.01% - 0.1%, which is used for crop seedling raising.

[0111] Applicable types: Solanaceous vegetables and Gramineae rice have strong root regeneration ability and are very suitable for seedling raising; for melons, generally seedling raising measures are taken when the temperature is low, and bitter gourds, loofahs, etc. can be directly sown in summer and autumn; for leafy vegetables, seedling raising is carried out according to specific cultivation technical measures; for leguminous vegetables, generally the direct seeding method is used; for root vegetables, seedling raising is not suitable.

[0112] (2) Mix and compound the polycyclic polyphenol solution with nutrient soil, aiming at facilitating the extension and attachment of plant roots to give full play to its fixing and retaining functions; it can provide good conditions such as water, fertilizer, air, heat, and pH value for plant roots to give full play to its role similar to that of soil. At the same time, it is widely used in the fields of overcoming continuous cropping obstacles of facility soil, pollution-free food production, and desertification transformation. According to the bag cultivation mode, the amount of nutrient soil used per mu is 20 cubic meters (20,000 liters), the required concentration of polycyclic polyphenols is 0.1% - 0.5%, and the ratio is 50 - 150 ml, which is used for crop cultivation.

[0113] Test Example 4 Mixing the polycyclic polyphenols prepared in the examples of the present invention with copper preparations

[0114] (1) Compound polycyclic polyphenols with copper preparations (Kesade 3000, Thaisen copper) in a specific ratio to maintain the uniformity of the compound solution.

[0115] Product type: Compound 1% polycyclic polyphenol solution with pesticides such as copper hydroxide, thiodiazole copper, thaisen copper, mancozeb, etc.

[0116]

[0117] For the aqueous solution system, the concentration of Cu in the copper hydroxide solution is 0.66 g / L, and the total Cu concentration in the MP - copper hydroxide solution is 0.33 g / L. In the polyphenol (MP) compound system, the Cu concentration in the MP - copper hydroxide test filtrate is 38.700 mg / L. Therefore, the Cu concentration in the precipitate is 291.3 mg / L. Thus, the amount of Cu adsorbed by 1 ml of 1% MP solution is 0.582 g; 2+ Concentration of total Cu in the MP - copper hydroxide solution is 0.33 g / L 2+ In the polyphenol (MP) compound system, the Cu concentration in the MP - copper hydroxide test filtrate is 38.700 mg / L. Therefore, the Cu concentration in the precipitate is 291.3 mg / L. Thus, the amount of Cu adsorbed by 1 ml of 1% MP solution is 0.582 g; 2+ Concentration of Cu in the precipitate is 291.3 mg / L; 2+ Thus, the amount of Cu adsorbed by 1 ml of 1% MP solution is 0.582 g; 2+

[0118] Calculation steps:

[0119] Concentration of Cu in B1: [(64 / 97.56)*1] g / 1L = 0.66 g / L 2+

[0120] Concentration of Cu in C1: (0.66 / 2) g / L = 0.33 g / L = 330 mg / L 2+

[0121] Concentration of Cu in the C1 precipitate: 330 mg / L - 38.700 mg / L = 291.3 mg / L 2+

[0122] Therefore, the capture rate of Cu2+ in copper hydroxide by 1% GO solution is: (291.3 / 330)*100% = 88.3%

[0123] Calculated in the same way:

[0124] The capture rate of Mn2+ in mancozeb by 1% GO solution is: 5.3%

[0125] The capture rate of Cu2+ in thiodiazole copper by 1% GO solution is: 92.4%

[0126] The capture rate of Cu2+ in thaisen copper by 1% GO solution is: 93.7%

[0127] Qualitative experiment: Use polycyclic polyphenols as a pharmaceutical intermediate, compound with copper preparations, to penetrate and transfer to the lesions of pests and diseases, inhibit the reproduction of bacteria, and prevent the soil - borne diseases of peanuts.

[0128] The investigation results of the control effect are as Figure 2 shown below.

[0129] Conclusion: Through three applications of pesticides, the incidence of bacterial wilt in peanuts decreased by 58%, the incidence of basal stem rot decreased by 58.4%, the incidence of root rot decreased by 39.7%, and the incidence of crown rot decreased by 41.7%. The test shows that the polycyclic polyphenol compound reagent has an obvious control effect on soil-borne diseases of peanuts.

[0130] Test Example 5 Spraying and injection of the polycyclic polyphenol prepared in the embodiment of the present invention to improve the yield and quality of edible fungi

[0131] Dilute the 1-1.2% polycyclic polyphenol solution at a ratio of 1:1000, and then spray and inject.

[0132] Application method: After diluting the polycyclic polyphenol (1.2%) solution, spray or inject it into the edible fungus bags. Dilute the polycyclic polyphenol at a ratio of 1:1000 and apply 2-5 ml to each bag by injection.

[0133] The experimental result data are as Figure 3 shown below.

[0134] As Figure 3 can be seen, the polycyclic polyphenol of the present invention can bring two benefits to the production of Tremella fuciformis:

[0135] (1) It can improve the quality of Tremella fuciformis, and the yield of Tremella fuciformis also increases by 8.86% (counting 600 pieces of Tremella fuciformis).

[0136] (2) It significantly reduces the number of molds at the roots of the finished Tremella fuciformis, achieving the effect of reducing the production of defective products caused by mold infection.

[0137] Test Example 6 Mixing the polycyclic polyphenol prepared in the embodiment of the present invention with a preservative

[0138] (1) Uniformly mix the polycyclic polyphenol with sugar, gibberellin, cytokinin, salicylic acid, abscisic acid, citric acid, hydroxyquinoline, hydroxyquinoline-derived salts, etc. The content of the polycyclic polyphenol should be 0.5% - 1% (m / v), and the pH value of the prepared aqueous solution is 4 - 7 (10°C - 35°C).

[0139] Product application: Using the polycyclic polyphenol as a new type of fungicide (fresh cut flower preservative) can extend the lifespan of fresh cut flowers;

[0140] A fresh cut flower preservative prepared with the polycyclic polyphenol, sugar, gibberellin, cytokinin, salicylic acid, abscisic acid, citric acid, hydroxyquinoline, hydroxyquinoline-derived salts, etc. as the main components, and the content of the polycyclic polyphenol is 0.5% - 1% (m / v).

[0141] In the cut rose flower experiment, the treatment of 200 mg / L SA + 3% sucrose + 300 mg / L citric acid had the longest effect, reaching 17.8 days. In the cut flower experiment of Delphinium grandiflorum 'Summer Skies', the preservation effect of 1% sucrose + 200 mg / L 8-HQS + 50 mg / L AgNO3 + 50 mg / L Al2(SO4)3 was the best.

[0142] Product indicators:

[0143]

[0144]

[0145] The experimental results are as Figure 4 , Figure 5 shown.

[0146] It can be Figure 4 seen that when fresh cut rose flowers are treated with 1% polycyclic polyphenol solution, the treated roses are larger in diameter than those in the control group. The lifespan of cut rose flowers separated from parent plants without external nutrition is only about 4 days (Control), and obvious rose neck bending phenomenon begins to appear on the fourth day. Compared with the untreated ones, the lifespan of the cut rose flowers treated with the solution is significantly extended by 3 - 7 days, and then neck bending and petal wilting occur.

[0147] It can be Figure 5 seen that the RFWs (relative fresh weight of cut flowers) of cut rose flowers treated with different doses of polycyclic polyphenols. The RFWs of roses treated with low concentrations of polycyclic polyphenols (polycyclic polyphenol 0.1, polycyclic polyphenol 1, and polycyclic polyphenol 10) are all higher than those in the control group and gradually decrease with the increase of the polycyclic polyphenol concentration. In contrast, the RFWs of roses treated with high concentration polycyclic polyphenol 100 decrease compared with the control group. The change trend of the RFWs of cut rose flowers treated with Chrysal sold on the market is similar to that treated with polycyclic polyphenols. However, the RFWs of cut rose flowers co-treated with Chrysal and polycyclic polyphenol 0.1 are significantly higher than those of Chrysal, indicating that the water absorption of roses is much greater than their water loss.

[0148] Polycyclic polyphenols have excellent properties due to their carbon-based surfaces and oxygen-containing functional groups. It has a bactericidal effect, can effectively inhibit the growth of microorganisms at the stem end of cut flowers, and improve the water absorption rate and water balance of cut rose flowers. Therefore, it can be used as a promising preservative to improve the ornamental value of cut flowers.

[0149] In summary, polycyclic polyphenols increase the activity of H+-ATPase on the plasma membrane of plants, pump H+ into the cell wall, resulting in the acidification and loosening of the cell wall. The H+-ATPase on the plasma membrane (PM) of plants is a main enzyme that can be used as an H+ pump to generate an electrochemical gradient of H+ on the PM, which can export H+ from plant and fungal cells to acidify the surrounding environment and generate a membrane potential, thereby stimulating the transport of inorganic ions (such as K+), metabolites and water in and out of cells through other transport systems. Plant cells are surrounded by a rigid cell wall and soften when acidified. Overexpression of the gene encoding PM H-ATPase can stimulate the hydraulic mechanism, which drives the opening of stomata, thereby promoting carbon monoxide absorption; at the same time, the main effect of overexpression is the improvement of stomatal conductance, the increase of transcription efficiency, the increase of photosynthetic rate, thereby leading to an increase in biomass and an increase in plant yield.

[0150] In the description of the present application, it should be understood that the terms "first", "second", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances. In addition, in the description of the present application, unless otherwise specified, "a plurality of" means two or more. "And / or" describes the associated relationship of the associated objects, indicating that three relationships can exist. For example, A and / or B can mean that A exists alone, A and B exist simultaneously, and B exists alone. The character " / " generally means that the associated objects before and after are in an "or" relationship.

[0151] The above-disclosed are only the preferred embodiments of the present application, and of course, the scope of the rights of the present application cannot be limited thereby. Therefore, the equivalent changes made according to the claims of the present application still fall within the scope covered by the present application.

Claims

1. A polycyclic polyphenol structure, characterized in that, The polycyclic polyphenol is composed of one or more cluster structures connected to each other. The cluster structure is composed of a parent nucleus structure formed by a carbocyclic ring or a heterocyclic ring and oxygen-containing functional groups covalently connected to the ring of the parent nucleus structure. The oxygen-containing functional groups are any one or more of hydroxyl group, carboxyl group, carbonyl group, epoxy group, ester group, ether bond, nitro group, alkoxy group, amide group, and sulfonyl group.

2. The polycyclic polyphenol structure according to claim 1, wherein The heteroelements in the heterocyclic ring include at least one of oxygen, nitrogen, phosphorus, and sulfur elements.

3. The polycyclic polyphenol structure according to claim 2, characterized in that, The number of rings of the carbocyclic ring or the heterocyclic ring is 3-12.

4. The polycyclic polyphenol structure according to claim 3, characterized in that, When the parent nucleus structure is a three-membered ring, the carbocyclic ring or the heterocyclic ring includes a three-membered carbocyclic ring containing only carbon elements, or a three-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The three-membered carbon heterocyclic ring includes a three-membered carbon heterocyclic ring composed only of single bonds or a three-membered carbon heterocyclic ring containing one double bond; When the parent nucleus structure is a four-membered ring, the carbocyclic ring or the heterocyclic ring includes a four-membered carbocyclic ring containing only carbon elements, or a four-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The four-membered carbon heterocyclic ring includes a four-membered carbon heterocyclic ring composed only of single bonds or a four-membered carbon heterocyclic ring containing one or two double bonds; When the parent nucleus structure is a five-membered ring, the carbocyclic ring or the heterocyclic ring includes a five-membered carbocyclic ring containing only carbon elements, or a five-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The five-membered carbon heterocyclic ring includes a five-membered carbon heterocyclic ring composed only of single bonds or a five-membered carbon heterocyclic ring containing one or two double bonds; When the parent nucleus structure is a six-membered ring, the carbocyclic ring or the heterocyclic ring includes a six-membered carbocyclic ring containing only carbon elements, or a six-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The six-membered carbon heterocyclic ring includes a six-membered carbon heterocyclic ring composed only of single bonds or a six-membered carbon heterocyclic ring containing one or two or three double bonds; When the parent nucleus structure is a seven-membered ring, the carbocyclic ring or the heterocyclic ring includes a seven-membered carbocyclic ring containing only carbon elements, or a seven-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The seven-membered carbon heterocyclic ring includes a seven-membered carbon heterocyclic ring composed only of single bonds or a seven-membered carbon heterocyclic ring containing one or two or three double bonds; When the parent nucleus structure is an eight-membered ring, the carbocyclic ring or the heterocyclic ring includes an eight-membered carbocyclic ring containing only carbon elements, or an eight-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The eight-membered carbon heterocyclic ring includes an eight-membered carbon heterocyclic ring composed only of single bonds or an eight-membered carbon heterocyclic ring containing one or more double bonds; When the parent nucleus structure is a nine-membered ring, the carbocyclic ring or the heterocyclic ring includes a nine-membered carbocyclic ring containing only carbon elements, or a nine-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The nine-membered carbon heterocyclic ring includes a nine-membered carbon heterocyclic ring composed only of single bonds or a nine-membered carbon heterocyclic ring containing one or more double bonds; When the parent nucleus structure is a ten-membered ring, the carbocyclic ring or the heterocyclic ring includes a ten-membered carbocyclic ring containing only carbon elements, or a ten-membered carbon heterocyclic ring composed of carbon elements and at least one heteroelement among oxygen, nitrogen, phosphorus, and sulfur. The ten-membered carbon heterocyclic ring includes a ten-membered carbon heterocyclic ring composed only of single bonds or a ten-membered carbon heterocyclic ring containing one or more double bonds; When the parent nucleus structure is an eleven-membered ring, the carbocyclic or carbocyclic-heterocyclic ring includes an eleven-membered carbocyclic ring containing only carbon elements, or an eleven-membered carbocyclic-heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The eleven-membered carbocyclic-heterocyclic ring includes an eleven-membered carbocyclic-heterocyclic ring composed only of single bonds or an eleven-membered carbocyclic-heterocyclic ring containing one or more double bonds. When the parent nucleus structure is a twelve-membered ring, the carbocyclic or carbocyclic-heterocyclic ring includes a twelve-membered carbocyclic ring containing only carbon elements, or a twelve-membered carbocyclic-heterocyclic ring composed of carbon elements and at least one hetero element among oxygen, nitrogen, phosphorus, and sulfur. The twelve-membered carbocyclic-heterocyclic ring includes a twelve-membered carbocyclic-heterocyclic ring composed only of single bonds or a twelve-membered carbocyclic-heterocyclic ring containing one or more double bonds.

5. The polycyclic polyphenol structure according to claim 4, wherein, When the parent nucleus structure is a three-membered ring, the parent nucleus structure is selected from any one of cyclopropane or cyclopropene; when the parent nucleus structure is a four-membered ring, the parent nucleus structure is selected from any one of cyclobutane, cyclobutene, or 1,3-cyclobutadiene; when the parent nucleus structure is a five-membered ring, the parent nucleus structure is selected from any one of cyclopentane, cyclopentene, 1,3-cyclopentadiene, furan, thiophene, or pyrrole; when the parent nucleus structure is a six-membered ring, the parent nucleus structure is selected from any one of cyclohexane, cyclohexene, 1,3-cyclohexadiene, 1,4-cyclohexadiene, benzene ring, pyran, pyridine, or pyrimidine; when the parent nucleus structure is a seven-membered ring, the parent nucleus structure is selected from any one of cycloheptane, cycloheptene, 1,3-cycloheptadiene, 1,3,5-cycloheptatriene, oxepane, or 1H-azepine; when the parent nucleus structure is an 8- to 12-membered ring, the parent nucleus structure is selected from a saturated carbocyclic or carbocyclic-heterocyclic ring or an unsaturated carbocyclic or carbocyclic-heterocyclic ring.

6. The polycyclic polyphenol structure according to claim 1, characterized in that, The cluster structure is one or more.

7. The polycyclic polyphenol structure according to claim 6, wherein, When there are multiple cluster structures, the connection modes between the carbocyclic or carbocyclic-heterocyclic rings in the cluster structure include bond connection, fused-ring connection, and spiro-ring connection.

8. The polycyclic polyphenol structure according to claim 7, characterized in that, The bond connection between rings includes bond connections composed of the same elements among carbon, oxygen, nitrogen, phosphorus, and sulfur and bond connections composed of different elements; the fused-ring connection between rings includes one fused-ring mode between two rings, two fused-ring modes between three rings, four fused-ring modes between four rings, and when there are more than three rings, the fused-ring modes along the direction in which the ring structures are arranged in a row or in a direction perpendicular thereto.

9. The polycyclic polyphenol structure according to claim 1, wherein The average carbon-oxygen ratio of the polycyclic polyphenol is 1.1 - 2.

0.

10. Application of the polycyclic polyphenol structure according to any one of claims 1 - 9 in agricultural production.

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