MiERF109-like or MiERF113 protein and application of coding gene thereof in mango fruit softening regulation

By silencing the MiERF109-like or MiERF113 gene in mango fruits, inhibiting its expression, solving the problem of rapid softening of mango fruits, achieving delayed softening, and providing a new technical solution for preservation and variety cultivation.

CN120330210APending Publication Date: 2025-07-18GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510438217.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-09
Publication Date
2025-07-18

AI Technical Summary

Technical Problem

The mango fruit softens rapidly during ripening, resulting in a shortening of the post-harvest shelf life, affecting long-distance transportation and annual balanced supply. The molecular mechanism of ERF family members in mango softening in the existing technology has not been fully analyzed.

Method used

The MiERF109-like or MiERF113 gene in mango fruits is silenced through VIGS technology to inhibit its expression. The silencing vector is constructed using the MiERF109-like or MiERF113 protein and its specific fragments of the encoding gene and introduced into Agrobacterium, thereby inhibiting the softening of mango fruits.

Benefits of technology

Delaying the softening of mango fruits provides technical means to cultivate hard meat, store-resistant new varieties and post-harvest freshness, providing an experimental basis for analyzing the physiological functions and mechanisms of mango fruit ripening and softening.

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Abstract

The invention discloses an application of a MiERF109-like or MiERF113 protein and a coding gene of the MiERF109-like or MiERF113 protein in regulation and control of softening of mango fruits. The expression mode of MiERF109-like or MiERF113 changing along with time is detected through real-time fluorescent quantitative PCR, and it is found that the expression mode of MiERF109-like or MiERF113 is closely related to the mango fruit softening process. According to the invention, a VIGS technology is further utilized to silence the ethylene response factor of the mango fruit, and results show that the hardness index of the fruit with the silent gene MiERF109-like or MiERF113 of the mango fruit is significantly changed, which indicates that the MiERF109-like or MiERF113 gene is a key gene affecting the softening of the mango fruit, and the Mango fruit with the silent gene miERF109-like or MiERF113 gene is a key gene affecting the softening of the mango fruit. The method can be applied to regulation and control of softening of mango fruits and cultivation of new storage and transportation-resistant mango varieties, and a new idea is provided for preservation of harvested mangoes.
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