Anti-aging skin-care matrix capable of repairing cell activity and preparation method of anti-aging skin-care matrix

Abstract

The invention relates to an anti-aging skin-care matrix capable of repairing cell activity and a preparation method of the anti-aging skin-care matrix. The composition is specifically prepared from the following raw materials in parts by mass: 10 to 30 percent of nicotinamide adenine dinucleotide NAD < + > precursor, 5 to 15 percent of phospholipid modified fullerene, 1 to 5 percent of oligopeptide-3 solution, 3 to 8 percent of vitamin C magnesium phosphate, 2 to 6 percent of vitamin E acetate, 2 to 6 percent of plant stem cell extract prepared from apple tooth powder, 0.5 to 2 percent of sodium hyaluronate, 5 to 10 percent of glycerinum and the balance of water. 1-5% of microcrystalline cellulose, 0.5-2% of sodium carboxymethyl starch, 0.1-1% of magnesium stearate, 10-19% of vegetable fat, 0.1-1% of perfume and the balance of deionized water. Through the synergistic effect of all the components, the effects of accelerating metabolism, helping to homogenize the skin color, reducing darkness, resisting aging and wrinkles, fading fine wrinkles and improving skin elasticity are achieved.

Description

Technical Field

[0001] The present invention relates to the fields of biomedicine and cosmetics, and particularly to an anti-aging skin care matrix for repairing cell activity and a preparation method thereof. Background Art

[0002] Aging is a natural physiological process. As people age, the activity of human cells gradually decreases, metabolism slows down, leading to a series of aging-related symptoms. At the cellular level, aging cells exhibit various abnormal changes, such as mitochondrial dysfunction, increased oxidative stress, telomere shortening, DNA damage accumulation, and cell cycle arrest. These changes not only affect the normal functions of cells but also cause the decline of tissue and organ functions, such as skin relaxation, increased wrinkles, decreased immunity, and reduced cardiovascular function.

[0003] In the prior art, there have been various studies and products targeting aging problems. In the field of cosmetics, common anti-aging ingredients include vitamin C, vitamin E, hyaluronic acid, collagen, etc. Vitamin C and vitamin E have antioxidant effects, which can scavenge free radicals in the body and reduce the damage of oxidative stress to cells. However, their effects are relatively single and it is difficult to comprehensively repair cell activity. Hyaluronic acid mainly plays a moisturizing role, increasing the water content of the skin and improving the dry state of the skin, but its effect on the internal activity repair of cells is limited. Collagen can supplement the collagen content in the skin, making the skin firmer. However, as people age, the body's ability to absorb and utilize collagen gradually decreases, and its effect is also limited to a certain extent.

[0004] In the fields of pharmaceuticals and health products, there are also some anti-aging products, such as some hormonal drugs, antioxidants, and plant extracts. Although hormonal drugs may improve aging symptoms to a certain extent, they have relatively large side effects, and long-term use may cause a series of health problems. Antioxidants such as coenzyme Q10 and astaxanthin can reduce the damage of oxidative stress to cells, but when used alone, their effect on repairing cell activity is not comprehensive enough. Plant extracts such as grape seed extract and green tea extract contain various antioxidant components. However, due to the complex composition, their action mechanisms are not clear, and the quality of plant extracts from different sources varies greatly.

[0005] In summary, although there are a wide variety of anti-aging products on the market at present, most products cannot comprehensively repair cell activity from multiple dimensions to achieve an ideal anti-aging effect. Therefore, it is of great market demand and practical application value to develop an anti-aging skin care matrix that can effectively repair cell activity, comprehensively delay aging, and is safe and reliable. Summary of the Invention

[0006] The primary object of the present invention is to provide an anti-aging skin care matrix for repairing cell activity, and this composition is achieved through the synergistic effect of multiple active ingredients.

[0007] Another object of the present invention is to provide a preparation method for the above-mentioned anti-aging skin care matrix for repairing cell activity. This method is simple to operate, has a stable process, and is suitable for large-scale industrial production.

[0008] An anti-aging skin care matrix for repairing cell activity, by weight percentage, consists of the following components:

[0009] Nicotinamide adenine dinucleotide NAD + Precursor 10 - 30%, phospholipid-modified fullerene 5 - 15%, oligopeptide-3 solution 1 - 5%, magnesium ascorbyl phosphate 3 - 8%, vitamin E acetate 2 - 6%, plant stem cell extract prepared from apple tooth powder 2 - 6%, sodium hyaluronate 0.5 - 2%, glycerol 5 - 10%, microcrystalline cellulose 1 - 5%, sodium carboxymethyl starch 0.5 - 2%, magnesium stearate 0.1 - 1%, vegetable fat 10% - 19%, fragrance 0.1% - 1%, and the balance is deionized water.

[0010] In the above formula, NAD + is an important coenzyme in cells and participates in various metabolic reactions in cells. As age increases, the intracellular NAD + level gradually decreases. Supplementing the NAD + precursor can increase the intracellular NAD + level, activate the Sirtuins protein family in cells, thereby repairing cell activity and delaying cell aging. Nicotinamide adenine dinucleotide NAD + precursor, as the core active ingredient, has the benefits of enhancing the body's immunity, improving nerve cell function, promoting sugar and lipid metabolism, and repairing DNA damage.

[0011] Further, the nicotinamide adenine dinucleotide precursor is at least one of nicotinamide mononucleotide and nicotinamide ribose.

[0012] Further, the nicotinamide adenine dinucleotide precursor is preferably nicotinamide mononucleotide.

[0013] Further, the weight percentage of the nicotinamide adenine dinucleotide precursor is preferably 15 - 25%.

[0014] In the above formula, the phospholipid-modified fullerene has super antioxidant ability, can effectively scavenge free radicals in the body, reduce the damage of oxidative stress to cells, and at the same time has an anti-inflammatory effect, can improve the microenvironment of cells, and promote cell repair and regeneration. As the core active ingredient, the phospholipid-modified fullerene has the benefits of promoting collagen production, making the skin more firm, delaying aging, being elastic, scavenging free radicals, and protecting cells.

[0015] Furthermore, the phospholipid-modified fullerene is obtained by surface modification of fullerene with amino-containing phospholipids to improve its dispersibility and stability in water.

[0016] Furthermore, the specific operation of surface modification of the phospholipid-modified fullerene is as follows:

[0017] Mix fullerene with amino-containing phospholipids, add chloroform according to the mass-volume ratio of 1 g:10 ml, and stir and react for 3 hours under the condition of an ultrasonic power of 300 W. Subsequently, rotate and evaporate to remove chloroform through a rotary evaporator at 40 °C and a vacuum degree of 0.08 MPa. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining the phospholipid-modified fullerene.

[0018] Furthermore, the mass ratio of fullerene to amino-containing phospholipids is 1:1 - 1:3.

[0019] Preferably, the amino-containing phospholipid is phosphatidylethanolamine.

[0020] Furthermore, the weight percentage of the phospholipid-modified fullerene is preferably 8 - 12%.

[0021] In the above formula, the oligopeptide-3 solution can promote the synthesis of substances such as collagen, has certain repair and anti-aging effects, can stimulate cell renewal and regeneration, and improve skin elasticity. It has the benefits of stimulating collagen production, repairing damaged skin, enhancing the barrier, improving skin barrier function, enhancing water-locking ability, relieving dryness and sensitivity, brightening skin tone, accelerating metabolism, helping to even out skin tone, reducing dullness, anti-aging and anti-wrinkle, fading fine lines, improving skin elasticity, and delaying aging.

[0022] Furthermore, the oligopeptide-3 solution is specifically prepared by dissolving oligopeptide-3 in phosphate buffer solution and adjusting the pH value to 6.5 - 7.5, and its concentration is 1 - 5 mg / mL.

[0023] Furthermore, the weight percentage of the oligopeptide-3 solution is preferably 2 - 4%.

[0024] In the above formula, magnesium ascorbyl phosphate is a stable derivative of vitamin C and has antioxidant effects. It has the benefits of synergistically scavenging free radicals with fullerene, promoting the synthesis of collagen, and enhancing the elasticity of the skin.

[0025] Furthermore, the weight percentage of magnesium ascorbyl phosphate is preferably 4 - 6%.

[0026] In the above formula, vitamin E acetate is a fat-soluble vitamin and has antioxidant effects. It has the benefits of protecting cell membranes from free radical damage, promoting blood circulation in the skin, and improving the nutritional supply of the skin.

[0027] Furthermore, the weight percentage of vitamin E acetate is preferably 3 - 5%.

[0028] In the above formula, the plant stem cell extract contains various bioactive components, such as polysaccharides, flavonoids, polyphenols, etc., and has antioxidant, anti-inflammatory, and cell regeneration-promoting effects. It has the benefit of providing nutrition for cells and enhancing the vitality of cells.

[0029] Furthermore, the plant stem cell extract prepared from apple tooth powder preferably uses apple stem cell extract, which is rich in proanthocyanidins, catechins, vitamin C, and superoxide dismutase, and has the benefit of delaying cell aging.

[0030] Furthermore, the plant stem cell extract prepared from apple tooth powder is prepared through the following steps:

[0031] Crush apple buds into particles of 2 - 5 mm and put them into a low-temperature vacuum extraction tank. Add extraction solvent ethanol in a ratio of 1:6 (g / mg). Control the temperature at 0 - 10°C, stir at a speed of 100 - 300 r / min for 2 - 6 hours, and conduct preliminary filtration through a microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 3000 - 8000 r / min and the centrifugation time at 10 - 30 minutes to make the residue precipitate at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it under the conditions of 30 - 45°C and a vacuum degree of 0.06 - 0.08 MPa. Use freeze-drying for drying. Place the product in a freeze-dryer, first pre-freeze it to -40 to -20°C and maintain it for 2 - 4 hours to completely freeze the material; then conduct sublimation drying under the condition of a vacuum degree lower than 10 Pa until the moisture content drops below 5% to obtain apple stem cell extract.

[0032] Furthermore, the weight percentage of the plant stem cell extract prepared from apple tooth powder is preferably 3 - 5%.

[0033] In the above formula, sodium hyaluronate has good moisturizing properties. It can absorb a large amount of water to keep the skin moist. At the same time, as a carrier for other active ingredients, it promotes their penetration and absorption.

[0034] Furthermore, the weight percentage of the sodium hyaluronate is preferably 1-1.5%.

[0035] In the above formula, glycerin is a commonly used humectant. It can increase the water content of the skin, improve the dry state of the skin, and also has a lubricating effect, making the product more comfortable to use.

[0036] Furthermore, the weight percentage of the glycerin is preferably 6-8%.

[0037] In the above formula, microcrystalline cellulose is used as an excipient, which can increase the volume of the composition, improve its fluidity and moldability, and facilitate the preparation of different dosage forms.

[0038] Furthermore, the preferred model of the microcrystalline cellulose is PH-102, which has a relatively large specific surface area and has the advantages of thickening and stabilizing, adsorbing grease, and improving the skin feel.

[0039] Furthermore, the weight percentage of the microcrystalline cellulose is preferably 2-4%.

[0040] In the above formula, sodium carboxymethyl starch has good disintegration properties. When preparing dosage forms such as tablets, it can quickly disintegrate the product and release the active ingredients.

[0041] Furthermore, the weight percentage of the sodium carboxymethyl starch is preferably 1-1.5%.

[0042] In the above formula, magnesium stearate can act as a lubricant. It can reduce the friction between particles, make the product more smooth during the preparation process, and prevent the product from caking during storage.

[0043] Furthermore, the weight percentage of the magnesium stearate is preferably 0.3-0.7%.

[0044] In the above formula, vegetable fat is a buffer among various components, which can prevent the product from stratifying and improve the comfort of using the product.

[0045] Furthermore, the vegetable fat is preferably coconut oil, which has good moisturizing and emollient properties.

[0046] Furthermore, the weight percentage of the vegetable fat is preferably 12-20%.

[0047] In the above formula, the fragrance has the advantage of improving the odor of the product and increasing the pleasure of use.

[0048] Further, the fragrance is preferably rose essence, which has the benefits of soothing emotions and assisting in antioxidant effects.

[0049] Further, the weight percentage of the fragrance is preferably 0.3 - 0.75%.

[0050] In the above formula, deionized water has good compatibility, high adaptability with various active ingredients, is not prone to chemical reactions, provides a pure carrier for the active ingredients, and helps the ingredients penetrate the skin. It has the benefits of enhancing the efficacy and improving the use feeling.

[0051] The preparation method of the repair cell activity anti-aging skin care matrix of the present invention includes the following steps:

[0052] S1. Ultrafinely crush nicotinamide mononucleotide in parts by mass of the formula using a jet mill, control the crushing pressure to be 5 - 10 MPa, the crushing time to be 10 - 20 minutes, and mix it with oligopeptide-3 solution and fullerene modified with phospholipids to obtain a pretreated core active ingredient.

[0053] S2. Add magnesium ascorbyl phosphate, vitamin E acetate and plant stem cell extract in parts by mass of the formula into deionized water respectively, and stir and dissolve at a temperature of 50 - 60 °C and a rotation speed of 200 - 300 r / min to obtain auxiliary active ingredients.

[0054] S3. Mix sodium hyaluronate and glycerol at a temperature of 30 - 40 °C and stir at a rotation speed of 100 - 200 r / min for 10 - 30 minutes. Then add microcrystalline cellulose, sodium carboxymethyl starch and magnesium stearate into the system and mix evenly according to the formula ratio to obtain an excipient mixture.

[0055] S4. Add the pretreated core active ingredient into the auxiliary active ingredient mixed solution, stir evenly, then add the excipient mixture, stir evenly, and adopt a wet granulation process to make particles with a particle size of 1 - 3 mm, and control the moisture content during the granulation process to be 10 - 15% to ensure the quality and formability of the particles.

[0056] S5. Put the particles obtained in step S4 into a drying oven and dry them at a temperature of 50 - 60 °C by hot air circulation drying until the moisture content is lower than 5%. Screen the dried particles to remove the adhered particles and fine powder to make the particle size more uniform. Use a vibrating screen for screening, and the screen aperture is 1 - 2 mm.

[0057] S6. After quality inspection and packaging, a repair cell activity anti-aging skin care matrix is obtained.

[0058] The repair cell activity anti-aging skin care matrix provided by the present invention and its preparation method have the following remarkable beneficial effects:

[0059] Magnesium ascorbyl phosphate, a stable derivative of vitamin C, can be slowly released as active vitamin C in the skin, directly neutralize free radicals such as hydroxyl radicals and superoxide anions, and reduce the oxidized fullerene, enabling its regeneration and continuous functioning to form an "antioxidant cycle". The unique cage-like structure of fullerene can efficiently capture singlet oxygen and nitrogen free radicals, directly blocking the free radical chain reaction, complementing vitamin C, covering various types of free radicals, and enhancing the antioxidant efficiency. Magnesium ascorbyl phosphate provides coenzymes for prolyl hydroxylase involved in collagen synthesis, directly promotes the formation of collagen fibers, inhibits the activity of matrix metalloproteinases, and reduces collagen degradation. Fullerene protects the collagen synthesis environment and enhances cell activity by scavenging free radicals to reduce the damage of inflammatory factor TNF-α to fibroblasts, indirectly synergistically enhancing the collagen synthesis efficiency with vitamin C.

[0060] The moist environment of sodium hyaluronate can enhance the dispersion stability of fullerene, preventing its inactivation due to aggregation. Encapsulating fullerene through liposome or microsphere technology forms a "fullerene reservoir" on the skin surface, slowly releasing and capturing free radicals. When small molecule sodium hyaluronate penetrates, it carries microemulsion particles to deliver vitamin E to the periphery of hair follicles and sebaceous glands, enhancing antioxidant protection. The moisturizing property of sodium hyaluronate maintains the skin's water-oil balance, and vitamin E specifically scavenges lipid free radicals. The two form a "water-oil biphasic antioxidant network" with a synergistic effect against lipid peroxidation. The porous network structure of sodium hyaluronate adsorbs polyphenol and polysaccharide components in apple stem cell extract through van der Waals forces to form a "gel-biological molecule complex". When applied, macromolecular sodium hyaluronate forms a film on the skin surface, slowly releasing the antioxidant components and stem cell activating factors in the extract. While sodium hyaluronate repairs the barrier, it provides a stable environment for apple stem cell extract, reducing the damage of external stimuli to the active substances. Detailed implementation manners

[0061] In order to make the objectives, technical solutions and advantages of the present invention more clear and understandable, the following further elaborates the present invention in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0062] All raw materials used in the following detailed implementation manners are of chemical purity. Among them, the model of the microcrystalline cellulose used is PH-102.

[0063] Example 1

[0064] A cell activity repairing and anti-aging skin care matrix is composed of the following components by weight percentage:

[0065] 20% nicotinamide mononucleotide, 10% phospholipid surface-modified fullerene, 3% oligopeptide-3 solution, 5% magnesium ascorbyl phosphate, 4% tocopheryl acetate, 4% apple stem cell extract, 1.2% sodium hyaluronate, 7% glycerol, 3% microcrystalline cellulose, 1.2% sodium carboxymethyl starch, 0.5% magnesium stearate, 15% plant fat, coconut oil, 0.5% fragrance, rose essence used, and the balance is deionized water.

[0066] Among them, the phospholipid surface-modified fullerene is specifically prepared by the following steps:

[0067] Mix fullerene and phosphatidylethanolamine according to the mass ratio of 1:2, add chloroform according to the mass-volume ratio of 1 g:10 ml, and stir and react for 3 hours under the condition of an ultrasonic power of 300 W. Subsequently, rotate and evaporate to remove chloroform through a rotary evaporator at 40 °C and a vacuum degree of 0.08 MPa. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining the phospholipid surface-modified fullerene.

[0068] The apple stem cell extract is specifically prepared by the following steps:

[0069] Crush apple buds into particles of 2 - 5 mm and put them into a low-temperature vacuum extraction tank. Add extraction solvent ethanol according to the ratio of 1:6 (g / mg), control the temperature at 5 °C, stir at a speed of 200 r / min for 5 hours, and preliminarily filter with a 0.45 μm microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 6000 r / min and the centrifugation time at 20 minutes to precipitate the residue at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it to 1 / 3 of the original volume at 40 °C and a vacuum degree of 0.07 MPa. Dry it by freeze-drying. Place the product in a freeze-dryer, first pre-freeze to -30 °C and keep it for 3 hours; then carry out sublimation drying under the condition of a vacuum degree of 0.0005 Pa until the moisture content drops below 5%, obtaining the apple stem cell extract.

[0070] A preparation method of a cell activity repair and anti-aging skin care matrix, comprising the following steps:

[0071] S1. Place nicotinamide mononucleotide in a jet mill, set the crushing pressure at 7 MPa and the crushing duration at 15 minutes. After detection, the obtained NMN particle size reaches about 3 μm, meeting the particle size requirements for improving bioavailability. Mix it with the oligopeptide-3 solution and the phospholipid-modified fullerene to obtain a pretreated core active ingredient;

[0072] Among them, the oligopeptide-3 solution is specifically prepared by the following steps: Weigh an appropriate amount of oligopeptide-3 accurately, slowly add it to the phosphate buffer solution, use a pH meter to adjust the pH value to 7.0, stir and dissolve to prepare a solution with a concentration of 3 mg / mL, and store it sealed in a 4°C refrigerator for later use.

[0073] S2. Weigh the raw materials of magnesium ascorbyl phosphate, vitamin E acetate, and apple stem cell extract and deionized water respectively, and stir at 55°C and 250 r / min for 15 minutes to obtain a mixed solution of auxiliary active ingredients.

[0074] S3. Mix sodium hyaluronate and glycerin, control the temperature at 35°C, and stir at a speed of 150 r / min for 30 minutes to obtain a sodium hyaluronate-glycerin solution. The solution is clear and transparent without obvious impurities. Subsequently, add microcrystalline cellulose, sodium carboxymethyl starch, and magnesium stearate to the system, mix evenly according to the formula ratio, set the mixing time to 20 minutes, and obtain an excipient mixture.

[0075] S4. Add the pretreated core active ingredient to the mixed solution of auxiliary active ingredients, stir evenly, then add the excipient mixture, stir at a speed of 350 r / min for 20 minutes, slowly add coconut oil and rose essence, continue to stir for 35 minutes, and granulate using an extrusion wet granulator. Control the moisture content at about 12% by adjusting the granulator, and discharge after making particles with a particle size of about 2 mm.

[0076] S5. Spread the obtained particles evenly on a drying tray, place them in a hot air circulation drying oven, set the drying temperature at 55°C, and the drying time at 3 hours. Turn the particles once every 1 hour to ensure uniform drying. After drying, it is detected that the moisture content of the particles drops below 4%. Screen the dried particles through a vibrating screen with a screen aperture of 1.5 mm to remove the agglomerated particles and fine powder, and obtain particles with a more uniform particle size.

[0077] S6. Conduct a comprehensive quality inspection on the sized particles. Use a laser particle size analyzer to detect the particle size distribution. The obtained particle size distribution is concentrated between 1.5 - 2.5 mm, meeting the product standard. Detect the moisture content with a moisture meter, and it is stably maintained at about 3%. Pack the qualified particles in bottles according to the specification of 100 g per bottle. The bottle body is made of a brown glass bottle, and the bottle cap is an aluminum-plastic combination cap.

[0078] Example 2

[0079] A cell activity repair and anti-aging skin care matrix, by weight percentage, consists of the following components:

[0080] 15% nicotinamide mononucleotide, 8% fullerene modified by phospholipid surface, 2% oligopeptide-3 solution, 4% magnesium ascorbyl phosphate, 3% vitamin E acetate, 3% apple stem cell extract, 1% sodium hyaluronate, 6% glycerin, 2% microcrystalline cellulose, 1% sodium carboxymethyl starch, 0.3% magnesium stearate, 15% vegetable fat, coconut oil, 0.5% fragrance, rose essence is used, and the balance is deionized water.

[0081] Among them, the fullerene modified by phospholipid surface is specifically prepared by the following steps:

[0082] Mix fullerene and phosphatidylethanolamine according to the mass ratio of 1:1, add chloroform according to the mass-volume ratio of 1g:10ml, and stir and react for 3 hours under the condition of ultrasonic power of 300W. Subsequently, rotate and evaporate to remove chloroform through a rotary evaporator at 40°C and a vacuum degree of 0.08MPa. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining fullerene modified by phospholipid surface.

[0083] The apple stem cell extract is specifically prepared by the following steps:

[0084] Crush apple buds into particles of 2-5mm and put them into a low-temperature vacuum extraction tank. Add extraction solvent ethanol according to the ratio of 1:6 (g / mg), control the temperature at 5°C, stir at a speed of 200r / min for 5 hours, and preliminarily filter with a 0.45μm microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 6000r / min and the centrifugation time at 20 minutes to precipitate the residue at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it to 1 / 3 of the original volume at 40°C and a vacuum degree of 0.07MPa. Dry it by freeze-drying. Place the product in a freeze-dryer, first pre-freeze to -30°C and keep it for 3 hours; then carry out sublimation drying under the condition of a vacuum degree of 0.001Pa until the water content drops below 5%, obtaining the apple stem cell extract.

[0085] A preparation method of a cell activity repair and anti-aging skin care matrix includes the following steps:

[0086] S1. A1. Place nicotinamide mononucleotide in a jet mill, set the crushing pressure at 5MPa, and the crushing duration at 20 minutes. After detection, the particle size of the obtained NMN reaches about 4μm, meeting the particle size requirements for improving bioavailability. Mix it with the oligopeptide-3 solution and the fullerene modified by phospholipid to obtain the pretreated core active ingredients.

[0087] Among them, the oligopeptide-3 solution is specifically prepared by the following steps: Weigh an appropriate amount of oligopeptide-3 accurately, slowly add it to the phosphate buffer solution, use a pH meter to adjust the pH value to 6.5, stir and dissolve to prepare a solution with a concentration of 2 mg / mL, and store it sealed in a 4°C refrigerator for later use.

[0088] S2. Respectively weigh the ingredients of magnesium ascorbyl phosphate, vitamin E acetate, apple stem cell extract, and deionized water, and add them to the stirring container in sequence. Control the temperature at 50°C and continuously stir at a speed of 200 r / min for 20 minutes to obtain the auxiliary active ingredient mixed solution.

[0089] S3. Mix sodium hyaluronate and glycerol, control the temperature at 35°C, and stir at a speed of 150 r / min for 30 minutes to obtain the sodium hyaluronate-glycerol solution. The solution is clear and transparent without obvious impurities. Subsequently, add microcrystalline cellulose, sodium carboxymethyl starch, and magnesium stearate to the system, mix them evenly according to the formula ratio, set the mixing time to 20 minutes, and obtain the excipient mixture.

[0090] S4. Add the pretreated core active ingredient to the auxiliary active ingredient mixed solution, stir evenly, then add the excipient mixture, stir at a speed of 350 r / min for 20 minutes, slowly add coconut oil and rose essence, continue to stir for 35 minutes, and use an extrusion wet granulator for granulation. Control the moisture content at about 10% by adjusting the granulator, and discharge after making particles with a particle size of about 1 mm.

[0091] S5. Spread the prepared particles evenly on the drying tray, put them into a hot air circulation drying oven, set the drying temperature at 50°C, and the drying time at 4 hours. Turn the particles once every 1 hour to ensure uniform drying. After drying, it is detected that the moisture content of the particles drops below 5%. Screen the dried particles through a vibrating screen with a screen aperture of 1 mm to remove the adhered particles and fine powder, and obtain particles with a more uniform particle size.

[0092] S6. Conduct a comprehensive quality inspection on the sized particles. Use a laser particle size analyzer to detect the particle size distribution. The obtained particle size distribution is concentrated between 1.0 - 2.05 mm, meeting the product standard. Detect the moisture content through a moisture meter, and it is stably maintained at about 3.5%. Package the qualified particles in bottles according to the specification of 100 g per bottle. The bottle body uses a brown glass bottle, and the bottle cap is an aluminum-plastic combined cap.

[0093] Example 3

[0094] A cell-repairing and anti-aging skin care matrix consists of the following components by weight percentage:

[0095] Nicotinamide mononucleotide 25%, phospholipid surface-modified fullerene 12%, oligopeptide-3 solution 4%, magnesium ascorbyl phosphate 6%, vitamin E acetate 5%, apple stem cell extract 5%, sodium hyaluronate 1.5%, glycerol 8%, microcrystalline cellulose 4%, sodium carboxymethyl starch 1.5%, magnesium stearate 0.7%, vegetable fat 15%, coconut oil and fragrance 0.5% are used, rose essence is used, and the balance is deionized water.

[0096] Among them, the phospholipid surface-modified fullerene is specifically prepared by the following steps:

[0097] Fullerene and phosphatidylethanolamine are mixed according to the mass ratio of 1:3, chloroform is added according to the mass-volume ratio of 1 g:10 ml, and the mixture is stirred and reacted for 3 hours under the condition of an ultrasonic power of 300 W. Subsequently, chloroform is removed by rotary evaporation at 40 °C and a vacuum degree of 0.08 MPa. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, thus obtaining the phospholipid surface-modified fullerene.

[0098] The apple stem cell extract is specifically prepared by the following steps:

[0099] Apple buds are crushed into particles of 2 - 5 mm and placed in a low-temperature vacuum extraction tank. Ethanol as the extraction solvent is added according to the ratio of 1:6 (g / mg). The temperature is controlled at 5 °C, and the mixture is stirred at a speed of 200 r / min for 5 hours, and then preliminarily filtered using a 0.45 μm microporous filter membrane. By means of low-temperature centrifugation, the centrifuge speed is set at 6000 r / min and the centrifugation time is 20 minutes to precipitate the residue at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. The separated extraction solution is transferred to a low-temperature rotary evaporator and concentrated to 1 / 3 of the original volume at 40 °C and a vacuum degree of 0.07 MPa. Freeze-drying is used for drying. The product is placed in a freeze-dryer, first pre-frozen to -30 °C and kept for 3 hours; then sublimation drying is carried out under the condition of a vacuum degree of 0.03 Pa until the water content drops below 5%, obtaining the apple stem cell extract.

[0100] A preparation method of a cell activity-repairing and anti-aging skin care matrix includes the following steps:

[0101] S1, A1: Nicotinamide mononucleotide is placed in a jet mill, the crushing pressure is set at 10 MPa, and the crushing duration is 10 minutes. After detection, the obtained NMN particle size reaches about 2 μm, meeting the particle size requirements for improving bioavailability. It is mixed with the oligopeptide-3 solution and the phospholipid-modified fullerene to obtain the pretreated core active ingredients.

[0102] Among them, the oligopeptide-3 solution is specifically prepared by the following steps: Weigh an appropriate amount of oligopeptide-3 accurately, slowly add it to the phosphate buffer solution, use a pH meter to adjust the pH value to 7.5, stir and dissolve to prepare a solution with a concentration of 4 mg / mL, and store it sealed in a 4°C refrigerator for later use.

[0103] S2: Weigh magnesium ascorbyl phosphate, vitamin E acetate, and apple stem cell extract raw materials in the formula portions, and 1.8% of deionized water by the formula weight, and add them to the stirring container in sequence. Control the temperature at 60°C and continuously stir at a speed of 300 r / min for 10 minutes to obtain an auxiliary active ingredient mixed solution.

[0104] S3: Mix sodium hyaluronate and glycerin, control the temperature at 40°C, and stir at a speed of 200 r / min for 30 minutes to obtain a sodium hyaluronate-glycerin solution. The solution is clear and transparent without obvious impurities. Subsequently, add microcrystalline cellulose, sodium carboxymethyl starch, and magnesium stearate to the system, mix evenly according to the formula ratio, set the mixing time to 20 minutes, and obtain an excipient mixture.

[0105] S4: Add the pretreated nicotinamide mononucleotide, phospholipid-modified fullerene, and oligopeptide-3 with a concentration of 4 mg / mL dissolved in PBS buffer solution to the auxiliary active ingredient mixed solution in sequence. Turn on the stirring device and stir at a speed of 350 r / min for 20 minutes to fully mix the core active ingredient and the auxiliary active ingredient. Slowly add coconut oil and rose essence, continue to stir for 35 minutes, and granulate using an extrusion wet granulator. Control the moisture content at about 15% by adjusting the granulator, and discharge after making granules with a particle size of about 3 mm.

[0106] S5: Spread the prepared granules evenly on the drying tray, put them into a hot air circulation drying oven, set the drying temperature at 60°C, and the drying time at 2 hours. Turn the granules once every 1 hour to ensure uniform drying. After drying, it is detected that the moisture content of the granules drops below 6%. Screen the dried granules through a vibrating screen with a screen aperture of 2 mm to remove the agglomerated granules and fine powder, and obtain granules with a more uniform particle size.

[0107] S6: Conduct a comprehensive quality inspection on the sized granules. Use a laser particle size analyzer to detect the particle size distribution. The obtained particle size distribution of the granules is concentrated between 2 - 3.5 mm, meeting the product standard. Detect the moisture content through a moisture meter, and it is stably maintained at about 4%.

[0108] Bottle-pack the qualified granules according to the specification of 50 g per bottle. The bottle body uses a brown glass bottle, and the bottle cap is an aluminum-plastic combination cap.

[0109] Comparative Example 1: Replace nicotinamide mononucleotide with fullerene

[0110] An anti-aging skin care matrix for repairing cell activity, by weight percentage, consists of the following components:

[0111] Fullerene modified by phospholipid surface 30%, oligopeptide-3 solution 3%, magnesium ascorbyl phosphate 5%, vitamin E acetate 4%, apple stem cell extract 4%, sodium hyaluronate 1.2%, glycerol 7%, microcrystalline cellulose 3%, sodium carboxymethyl starch 1.2%, magnesium stearate 0.5%, vegetable fat 15%, using coconut oil; fragrance 0.5%, using rose essence, and the balance is deionized water.

[0112] Among them, the fullerene modified by phospholipid surface is specifically prepared by the following steps:

[0113] Mix fullerene and phosphatidylethanolamine according to the mass ratio of 1:2, add chloroform according to the mass-volume ratio of 1g:10ml, and stir and react for 3 hours under the condition of ultrasonic power of 300W. Subsequently, remove chloroform by rotary evaporation at 40°C and a vacuum degree of 0.08MPa through a rotary evaporator. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining fullerene modified by phospholipid surface.

[0114] The apple stem cell extract is specifically prepared by the following steps:

[0115] Crush apple buds into particles of 2-5mm and put them into a low-temperature vacuum extraction tank. Add extraction solvent ethanol according to the ratio of 1:6 (g / mg), control the temperature at 5°C, stir at a speed of 200r / min for 5 hours, and preliminarily filter with a 0.45μm microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 6000r / min and the centrifugation time at 20 minutes to make the residue precipitate at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it to 1 / 3 of the original volume at 40°C and a vacuum degree of 0.07MPa. Dry it by freeze-drying. Place the product in a freeze-dryer, first pre-freeze to -30°C and keep it for 3 hours; then carry out sublimation drying under the condition of a vacuum degree of 0.0005Pa until the water content drops below 5%, obtaining the apple stem cell extract.

[0116] The preparation method adopts the same preparation method as in Example 1, and the parameters of each step are kept consistent.

[0117] Comparative Example 2: Replace fullerene with nicotinamide mononucleotide

[0118] An anti-aging skin care matrix for repairing cell activity, by weight percentage, consists of the following components:

[0119] 30% nicotinamide mononucleotide, 3% oligopeptide-3 solution, 5% magnesium ascorbyl phosphate, 4% tocopheryl acetate, 4% apple stem cell extract, 1.2% sodium hyaluronate, 7% glycerin, 3% microcrystalline cellulose, 1.2% sodium carboxymethyl starch, 0.5% magnesium stearate, 15% vegetable fat, using coconut oil; 0.5% fragrance, using rose essence, and the balance is deionized water.

[0120] Among them, the fullerene modified by phospholipid surface is specifically prepared by the following steps:

[0121] Mix fullerene and phosphatidylethanolamine according to the mass ratio of 1:2, add chloroform according to the mass-volume ratio of 1 g:10 ml, and stir and react for 3 hours under the condition of ultrasonic power of 300 W. Subsequently, remove chloroform by rotary evaporation at 40 °C and a vacuum degree of 0.08 MPa through a rotary evaporator. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining fullerene modified by phospholipid surface.

[0122] The apple stem cell extract is specifically prepared by the following steps:

[0123] Crush apple buds into particles of 2-5 mm and put them into a low-temperature vacuum extraction tank. Add extraction solvent ethanol according to the ratio of 1:6 (g / mg), control the temperature at 5 °C, stir at a speed of 200 r / min for 5 hours, and preliminarily filter with a 0.45 μm microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 6000 r / min and the centrifugation time at 20 minutes to precipitate the residue at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it to 1 / 3 of the original volume at 40 °C and a vacuum degree of 0.07 MPa. Dry it by freeze-drying. Place the product in a freeze-dryer, first pre-freeze to -30 °C and keep it for 3 hours; then carry out sublimation drying under the condition of a vacuum degree of 0.0005 Pa until the water content drops below 5%, obtaining the apple stem cell extract.

[0124] The preparation method adopts the same preparation method as in Example 1, and the parameters of each step are kept consistent.

[0125] Comparative Example 3: Replace oligopeptide-3 with fullerene

[0126] A cell activity repair and anti-aging skin care matrix, by weight percentage, consists of the following components:

[0127] Nicotinamide mononucleotide 20%, phospholipid surface-modified fullerene 13%, magnesium ascorbyl phosphate 5%, vitamin E acetate 4%, apple stem cell extract 4%, sodium hyaluronate 1.2%, glycerol 7%, microcrystalline cellulose 3%, sodium carboxymethyl starch 1.2%, magnesium stearate 0.5%, vegetable fat 15%, using coconut oil; fragrance 0.5%, using rose essence, and the balance is deionized water.

[0128] Among them, the phospholipid surface-modified fullerene is specifically prepared by the following steps:

[0129] Mix fullerene and phosphatidylethanolamine according to the mass ratio of 1:2, add chloroform according to the mass-volume ratio of 1g:10ml, and stir and react for 3 hours under the condition of an ultrasonic power of 300W. Subsequently, rotate and evaporate to remove chloroform through a rotary evaporator at 40°C and a vacuum degree of 0.08MPa. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining the phospholipid surface-modified fullerene.

[0130] The apple stem cell extract is specifically prepared by the following steps:

[0131] Crush apple buds into particles of 2 - 5mm and put them into a low-temperature vacuum extraction tank. Add the extraction solvent ethanol according to the ratio of 1:6 (g / mg), control the temperature at 5°C, stir at a speed of 200r / min for 5 hours, and preliminarily filter with a 0.45μm microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 6000r / min and the centrifugation time at 20 minutes to make the residue precipitate at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it to 1 / 3 of the original volume at 40°C and a vacuum degree of 0.07MPa. Dry it by freeze-drying. Place the product in a freeze-dryer, first pre-freeze to -30°C and keep it for 3 hours; then carry out sublimation drying under the condition of a vacuum degree of 0.0005Pa until the water content drops below 5%, obtaining the apple stem cell extract.

[0132] The preparation method adopts the same preparation method as in Example 1, and the parameters of each step are kept consistent.

[0133] Comparative Example 4: Replace fullerene and oligopeptide-3 with nicotinamide mononucleotide

[0134] A cell activity-repairing and anti-aging skin care matrix, by weight percentage, consists of the following components:

[0135] Nicotinamide mononucleotide 43%, magnesium ascorbyl phosphate 5%, vitamin E acetate 4%, apple stem cell extract 4%, sodium hyaluronate 1.2%, glycerin 7%, microcrystalline cellulose 3%, sodium carboxymethyl starch 1.2%, magnesium stearate 0.5%, vegetable fat 15%, using coconut oil; fragrance 0.5%, using rose essence, the balance is deionized water.

[0136] Among them, the fullerene modified by phospholipid surface is specifically prepared by the following steps:

[0137] Mix fullerene and phosphatidylethanolamine according to the mass ratio of 1:2, add chloroform according to the mass-volume ratio of 1g:10ml, and stir and react for 3 hours under the condition of ultrasonic power of 300W. Subsequently, rotate and evaporate to remove chloroform through a rotary evaporator at 40°C and a vacuum degree of 0.08MPa. Its dispersibility in water is significantly improved, and there is no obvious agglomeration phenomenon after standing for one week, obtaining fullerene modified by phospholipid surface.

[0138] The apple stem cell extract is specifically prepared by the following steps:

[0139] Crush apple buds into particles of 2-5mm and put them into a low-temperature vacuum extraction tank. Add extraction solvent ethanol according to the ratio of 1:6 (g / mg), control the temperature at 5°C, stir at a speed of 200r / min for 5 hours, and preliminarily filter with a 0.45μm microporous filter membrane. Use low-temperature centrifugation to set the centrifuge speed at 6000r / min and the centrifugation time at 20 minutes to precipitate the residue at the bottom of the centrifuge tube, obtaining a relatively clear extraction solution. Transfer the separated extraction solution to a low-temperature rotary evaporator and concentrate it to 1 / 3 of the original volume at 40°C and a vacuum degree of 0.07MPa. Dry it by freeze-drying. Place the product in a freeze-dryer, first pre-freeze to -30°C and keep it for 3 hours; then carry out sublimation drying under the condition of a vacuum degree of 0.0005Pa until the water content drops below 5%, obtaining the apple stem cell extract.

[0140] The preparation method adopts the same preparation method as in Example 1, and the parameters of each step are kept consistent.

[0141] Experimental Example 1

[0142] Select 150 volunteers aged between 40 and 60 years old with obvious skin aging symptoms (such as increased wrinkles, skin relaxation, dryness and roughness, etc.), and randomly divide them into 7 groups with 50 people in each group. Apply the repair cell activity anti-aging skin care matrix prepared in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4 to the skin at a dose of 0.5g twice a day for 3 months continuously. Detect the skin condition of the volunteers before use, after 1 month, 2 months, and 3 months of use.

[0143] Detection indicators and methods

[0144] Cell viability detection: Using the MTT method, after different compositions act on cells for 48 hours, the absorbance value of the cells is detected. The higher the absorbance, the stronger the cell viability.

[0145] Determination of free radical scavenging rate: Using the DPPH free radical scavenging experiment, the ability of the composition to scavenge DPPH free radicals is determined. The higher the scavenging rate, the better the antioxidant effect.

[0146] Detection of collagen synthesis amount: Using the ELISA method, the content of collagen in the cell culture supernatant is detected. The higher the content, the stronger the promoting effect on collagen synthesis.

[0147] The specific test results are shown in Tables 1-8 below.

[0148] Table 1 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Example 1 group

[0149]

[0150] Table 2 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Example 2 group

[0151]

[0152] Table 3 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Example 3 group

[0153]

[0154] Table 4 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Comparative Example 1 group

[0155]

[0156] Table 5 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Comparative Example 2 group

[0157]

[0158] Table 6 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Comparative Example 3 group

[0159]

[0160] Table 7 Average values of skin moisture content, elasticity, and wrinkle depth of volunteers in Comparative Example 4 group

[0161]

[0162] Table 8 Experimental Measurement Results of Example 1 and Comparative Examples 1-4

[0163]

[0164] As can be seen from Tables 1-7, after the seven groups of volunteers used the products, various skin indicators were improved to varying degrees. Among them, the percentage of effect optimization in Comparative Examples 1-4 was significantly lower than that in Examples 1-3. The average skin moisture content of the volunteers using the product of Example 1 increased by 25%, the elasticity increased by 20%, the average wrinkle depth decreased by 18%, and the gloss was significantly enhanced; the average skin moisture content of the volunteers using the product of Example 2 increased by 20%, the elasticity increased by 15%, and the average wrinkle depth decreased by 15%; the average skin moisture content of the volunteers using the product of Example 3 increased by 22%, the elasticity increased by 18%, and the average wrinkle depth decreased by 16%. Generally speaking, the anti-aging skin care matrix prepared in Example 1 was more prominent in improving skin aging symptoms, verifying the effectiveness and superiority of the anti-aging skin care matrix of the present invention.

[0165] As can be seen from Table 8, in terms of cell viability: the cell viabilities of Comparative Examples 1-4 were significantly lower than those of Examples 1-3. In Comparative Example 1, NMN was absent, and it was unable to effectively increase the intracellular NAD + level to activate the Sirtuins protein family, and the cell viability decreased significantly; in Comparative Example 2, fullerene was lacking, the cell microenvironment was insufficiently improved, and the antioxidant capacity was weakened, resulting in impaired cell viability; in Comparative Example 3, oligopeptide-3 was absent, cell renewal and collagen synthesis were affected, and it was difficult to maintain a high level of cell viability; in Comparative Example 4, fullerene and oligopeptide-3 were absent, and the cell viability was the lowest, indicating that the core active ingredients are the key to maintaining cell viability.

[0166] In terms of free radical scavenging rate: the free radical scavenging rate of Example 1 was the highest. In Comparative Example 2, due to the lack of fullerene, its antioxidant capacity decreased significantly; although Comparative Examples 1 and 3 had fullerene, due to the incomplete core components, the synergistic effect was limited, and the scavenging rate was lower than that of Example 1; Comparative Example 4 almost lost the core antioxidant components, and the scavenging rate was the lowest, fully reflecting the importance of the synergistic antioxidant effect of the core active ingredients.

[0167] In terms of the amount of collagen synthesized: Example 1 had the strongest promoting effect on collagen synthesis. In Comparative Example 3, the absence of oligopeptide-3 directly affected collagen synthesis; in Comparative Examples 1 and 2, due to the lack of some core components, the best synergistic promoting effect could not be achieved; in Comparative Example 4, the core components were absent, and the amount of collagen synthesized was the least, indicating that the core active ingredients must act together to effectively promote collagen synthesis.

[0168] The above comparative experiment results fully prove the accuracy and precision of the preferred range of the core active ingredients in the anti-aging skin care matrix for repairing cell activity of the present invention, as well as the integrity and synergy of nicotinamide mononucleotide, fullerene, and oligopeptide-3. They cooperate with each other to jointly repair cell activity and delay cell aging from multiple aspects. The lack of any one of the core ingredients will lead to a significant reduction in the anti-aging effect.

[0169] As described above, the above are only the preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

[0170] The above content is only an example and illustration of the present invention. Those skilled in the art of the present technology make various modifications or supplements or use similar methods to substitute the described specific embodiments, as long as they do not deviate from the invention or exceed the scope defined by the claims of the present invention, they should fall within the protection scope of the present invention.

Claims

1. A cell activity-repairing anti-aging skin care matrix, characterized in that, By weight percentage, it consists of the following components: nicotinamide adenine dinucleotide NAD + Precursor 10 - 30%, phospholipid-modified fullerene 5 - 15%, oligopeptide-3 solution 1 - 5%, magnesium ascorbyl phosphate 3 - 8%, tocopheryl acetate 2 - 6%, plant stem cell extract prepared from apple tooth powder 2 - 6%, sodium hyaluronate 0.5 - 2%, glycerol 5 - 10%, microcrystalline cellulose 1 - 5%, sodium carboxymethyl starch 0.5 - 2%, magnesium stearate 0.1 - 1%, vegetable fat 10% - 19%, fragrance 0.1% - 1%, and the balance is deionized water.

2. The anti-aging skin care matrix for repairing cell activity according to claim 1, characterized in that, The nicotinamide adenine dinucleotide NAD + precursor is nicotinamide mononucleotide.

3. The anti-aging skin care matrix for repairing cell activity according to claim 1, characterized in that, The phospholipid-modified fullerene is specifically obtained by subjecting fullerene to surface modification treatment with an amino group-containing phospholipid.

4. The anti-aging skin care matrix for repairing cell activity according to claim 3, characterized in that, The mass ratio of fullerene to the amino group-containing phospholipid is 1:1 - 1:

3.

5. The anti-aging skin care matrix for repairing cell activity according to claim 1, wherein The plant stem cell extract prepared from apple tooth powder is obtained through the following steps: After crushing apple buds, add them to an extraction solvent, control the system temperature at 0 - 10°C and stir for 2 - 6 hours. After filtration, continue centrifugation, discard the precipitate, and obtain an extract. Rotate and evaporate the extract to concentrate it, and then freeze-dry it. The freeze-drying procedure is specifically as follows: first pre-freeze to -40 to -20°C and maintain for 2 - 4 hours, and then perform sublimation drying until the moisture content drops below 5% to obtain the apple stem cell extract.

6. The anti-aging skin care matrix for repairing cell activity according to claim 1, wherein The oligopeptide-3 solution is specifically prepared by dissolving oligopeptide-3 in a phosphate buffer solution and adjusting the pH value to 6.5 - 7.5, and its concentration is 1 - 5 mg / mL.

7. A method for preparing a repair cell activity anti-aging skin care matrix according to any one of claims 1-6, characterized in that, It includes the following steps: S1. Pretreatment of the core active ingredient: Ultra-finely pulverize nicotinamide adenine dinucleotide NAD in terms of formula parts by mass + precursor, and then mix it with oligopeptide-3 solution and phospholipid-modified fullerene to obtain a pretreated core active ingredient; S2. Respectively add magnesium ascorbyl phosphate, tocopheryl acetate, and the plant stem cell extract in the formula parts by mass to deionized water, and stir at a temperature of 50 - 60°C to obtain the auxiliary active ingredient; S3. Mix sodium hyaluronate and glycerol at a temperature of 30 - 40°C, and then mix microcrystalline cellulose, sodium carboxymethyl starch, and magnesium stearate evenly in the system according to the formula ratio to obtain an excipient mixture; S4. Add the pretreated core active ingredient to the auxiliary active ingredient mixed solution, stir evenly, then add the excipient mixture, stir evenly, and use the wet granulation process to make granules; S5. Dry the granules prepared in step S4 and then perform sizing; S6. After quality inspection and packaging, a cell-repairing and anti-aging skin care matrix is obtained.

8. The preparation method of the anti-aging skin care matrix for repairing cell activity according to claim 6, characterized in that, For the ultrafine pulverization treatment of nicotinamide mononucleotide, set the pulverization pressure at 5 - 10 MPa and the pulverization time at 10 - 20 minutes.

9. The preparation method of the anti-aging skin care matrix for repairing cell activity according to claim 6, characterized in that, During the wet granulation process, control the moisture content at 10 - 15% to make granules with a particle size of 1 - 3 mm.

10. The preparation method of the anti-aging skin care matrix for repairing cell activity according to claim 6, characterized in that, The drying process uses a hot air circulation drying method, with a drying temperature of 50 - 60°C and a drying time of 2 - 4 hours.

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