Amino chiral metabolite resolution reagent and application thereof

By using TPP-R-BSA and TPP-S-BSA reagents to resolve the chiral compounds of amino functional groups under neutral conditions, the racemization and false positive problems of chiral compounds of amino functional groups in the prior art were solved, and high sensitivity and high selectivity amino acid resolution were achieved.

CN120398948APending Publication Date: 2025-08-01YANBIAN UNIV
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Patent Information

Application Number
CN202510528945.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-25
Publication Date
2025-08-01

AI Technical Summary

Technical Problem

When existing chiral derivatization reagents perform the separation of amino functional group chiral compounds under alkaline conditions, they are prone to racemic and false positive results, and the separation efficiency is low, making it difficult to meet the analysis needs of high sensitivity and high selectivity.

Method used

Compounds TPP-R-BSA and TPP-S-BSA containing positively charged triphenylphosphine groups and chiral center structures are used as the resolution reagents for chiral compounds of amino functional groups. The liquid chromatography-mass spectrometry detection technology is used to achieve high sensitivity and high selectivity resolution of chiral compounds of amino functional groups under neutral conditions.

Benefits of technology

The efficient separation of 13 chiral amino acids was achieved, with a resolution of 1.56-12.67, which significantly improved the mildness of derivatization, reduced the probability of racemization, and improved the accuracy and sensitivity of the analysis.

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Abstract

The invention provides an amino chiral metabolite resolution reagent and application thereof, and belongs to the technical field of biochemistry. The compound TPP-R-BSA and TPP-S-BSA reagents provided by the invention contain triphenylphosphine groups with positive charges and a chiral center structure, and can be used for performing mass spectrum labeling on amino functional group chiral compounds. According to the present invention, the chiral resolution of 13 chiral amino compounds can be simultaneously performed under the neutral condition for the first time, and the mild degree of the derivatization is significantly higher than the mild degree of the existing chiral derivatization reagent; according to the reagent, an analysis method which is mild in amino functional group chiral compound resolution, high in sensitivity and high in selectivity can be established by utilizing a liquid chromatography-mass spectrometry detection technology. And an effective and reliable mass spectrum chiral derivatization reagent is provided for the resolution research of amino functional group chiral metabolites and the screening of chiral biomarkers.
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