A specific culture medium for detecting propionibacterium acnes in cosmetics and a detection method thereof

By using antibiotic-free specific culture media and anaerobic culture methods, combined with selective inhibitors and chromogenic indicators, the problems of insufficient inhibitors and insensitive color development in existing Propionibacterium acnes culture media have been solved, achieving highly accurate and environmentally friendly detection of Propionibacterium acnes.

CN120442747BActive Publication Date: 2025-12-26GUANGZHOU ZHONGKE INSPECTION TECH TESTING CO LTD +2
4 Cites 0 Cited by

Patent Information

Application Number
CN202510592794.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-09
Publication Date
2025-12-26
Estimated Expiration
2045-05-09

AI Technical Summary

Technical Problem

Current Propionibacterium acnes culture media lack targeted inhibitors and cannot effectively inhibit the growth of Gram-positive bacteria such as lactobacilli, resulting in poor accuracy of test results, insensitive colorimetric reactions, and reliance on antibiotics, which may lead to drug resistance problems.

Method used

Using an antibiotic-free specific culture medium, Tween 80, tea tree oil extract, bile salts, sodium laurate, and hydroxyproline were added as selective inhibitors. Combined with bromocresol purple colorimetric indicator and anaerobic culture method, the growth of lactic acid bacteria and other bacteria was inhibited, while the specific growth and colorimetric detection of Propionibacterium acnes were promoted.

Benefits of technology

It improves the accuracy and reliability of Propionibacterium acnes detection, avoids antibiotic resistance problems, and has a sensitive and highly specific colorimetric reaction that meets environmental protection requirements.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The present application relates to a kind of specific culture medium for detecting propionibacterium acnes in cosmetic and its detection method, belong to microorganism detection technical field.The specific culture medium of the present application contains 5-25g nitrogen source per 1000mL culture medium, 5-10g carbon source, 0.02-16g inhibitor, 0.01-0.02g color indicator, 0.1-13g regulator, and the rest is solvent;The inhibition includes Tween 80, tea tree oil extract, bile salt, sodium laurate and hydroxyproline.The specific culture medium provided by the present application is a kind of antibiotic-free, high-selectivity, color-sensitive and low-cost propionibacterium acnes selective culture medium.By optimizing the composition of culture medium, adding selective inhibitor and color indicator, the growth of other facultative anaerobes such as lactic acid bacteria can be effectively inhibited, the specificity of propionibacterium acnes is cultured and color detection is carried out, and the accuracy and reliability of detection are improved.
Need to check novelty before this filing date? Find Prior Art