Low immunogenicity of lwcas13a protein mutant and application thereof

By mutating the LwCas13a protein with Q436C, K560D, and K76C mutations, the immunogenicity of the Cas13 protein in large mammals was resolved, achieving stable protein expression and efficient gene editing, and reducing the immune response.

CN120738153BActive Publication Date: 2026-01-16INNER MONGOLIA UNIVERSITY
2 Cites 0 Cited by

Patent Information

Application Number
CN202511269339.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-09-08
Publication Date
2026-01-16
Estimated Expiration
2045-09-08

AI Technical Summary

Technical Problem

Long-term expression of the Cas13 protein in large mammals raises immunogenicity concerns, as it may be recognized as a target of the immune system, leading to instability in immune responses and the regulation of targeted RNA.

Method used

We designed low-immunogenic LwCas13a protein mutants and introduced mutations such as Q436C, K560D, and K76C into the wild-type LwCas13a protein to reduce its affinity for bovine MHC-I molecules, thereby maintaining the protein's structural stability and nuclease activity.

Benefits of technology

It significantly reduced the immunogenicity of LwCas13a protein in cattle, ensuring long-term stable protein expression and the ability to regulate the target RNA, maintaining gene editing efficiency, and reducing the rejection response of the immune system.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The present application relates to the technical field of genetic engineering, and particularly relates to a low immunogenic LwCas13a protein mutant and application thereof.The LwCas13a protein mutant provided by the present application has one or more mutations of Q436C, K560D and K76C on the wild-type LwCas13a protein.The LwCas13a protein mutant provided by the present application significantly reduces the immunogenicity of the LwCas13a protein in a bovine body, thereby reducing the recognition and reaction of the immune system.The LwCas13a protein mutant provided by the present application not only does not affect the activity of the LwCas13a nuclease, but also maintains the effective expression and function of the LwCas13a nuclease in bovine cells.The gene editing efficiency of the LwCas13a protein mutant provided by the present application is equivalent to that of the wild type, and the interference efficiency is not significantly affected.
Need to check novelty before this filing date? Find Prior Art