Environment-friendly odorless sealing strip adhesive as well as preparation and application thereof

Abstract

The invention discloses an environment-friendly odorless sealing strip adhesive as well as preparation and application thereof, and the environment-friendly odorless sealing strip adhesive is prepared from the following components in parts by mass: 15 to 20 parts of polyacrylate resin, 10 to 15 parts of dammar resin, 5 to 10 parts of fir resin, 3 to 5 parts of hydrogenated terpene resin, 2 to 5 parts of tributyl citrate and 45 to 65 parts of n-decane. The preparation method comprises the following steps: uniformly mixing natural resin (dammar resin, fir resin and hydrogenated terpene resin), polymeric monomers (n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate) and an environment-friendly solvent (n-decane), and then polymerizing to obtain the environment-friendly adhesive. The synthetic resin (polyacrylate resin) and the natural resin are sufficiently and mutually dissolved in n-decane to form stable and uniform composite resin glue, so that the problems of health risk and environmental pollution caused by harmful solvents such as xylene in the traditional sealing glue are effectively avoided, and the characteristics of no toxicity, no odor and environmental protection of the product are realized; meanwhile, the stability and the bonding performance of the section sealing glue are improved, the matching performance of the optical transmittance and the refractive index of the section sealing glue is enhanced, and the section sealing glue has quick-drying performance and is suitable for medical pathological sections.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of pathological tissue section technology, and in particular to an environmentally friendly odorless mounting medium and a preparation and application thereof. BACKGROUND

[0002] In the process of preparing medical pathological tissue sections, mounting medium, as one of the key materials, is widely used to bond and seal the glass slide and the cover glass, so that the dyed tissue sample is stably preserved between the two for a long time, facilitating microscopic observation and subsequent research. A high-quality mounting medium not only needs to have good bonding performance and sealing performance, but also needs to have a refractive index close to that of glass to ensure the clarity of the image and the optical transmission performance. In addition, the mounting medium should have good chemical inertness and not react with the dyed tissue to avoid affecting the tissue morphology and staining effect.

[0003] However, the mounting medium widely used in clinical and scientific research at present is mostly a gum product based on natural neutral resin (such as dammar resin and fir resin), and its traditional preparation method is to use strong solvents such as xylene for dissolution and preparation. Although this type of product performs well in terms of transparency, biocompatibility and bonding performance, the solvent used has strong volatility and toxicity, and long-term contact can cause serious harm to human health, which has gradually failed to meet the requirements of modern medical environment for environmental protection and occupational health and safety. Therefore, developing an environmentally friendly, non-toxic and low-odor mounting medium with excellent optical performance and long-term stability has become a technical problem that needs to be solved in the field of pathological section at present. In view of the above problems, some manufacturers have tried to develop environmentally friendly alternative products based on synthetic resin, such as neutral environmentally friendly quick-drying glue or ultra-clean mounting medium. Although these products have abandoned toxic solvents such as xylene and adopted a more environmentally friendly system, they still have many shortcomings in actual application, such as low refractive index, easy discoloration of dyed tissue and poor stability, which makes it difficult to meet the needs of long-term preservation of pathological samples.

[0004] Therefore, it is urgent to develop a new type of environmentally friendly mounting medium and its preparation method, which can ensure excellent optical performance and bonding strength while achieving true environmental protection, and promote the development of pathological section preparation technology in a safer and more efficient direction. SUMMARY

[0005] Therefore, the present application provides an environmentally friendly odorless mounting medium and a preparation and application thereof, which are used to solve the problem of how to improve the comprehensive performance of the mounting medium to adapt to pathological section samples.

[0006] To achieve the above technical purpose, the present application adopts the following technical scheme: The first aspect of the present application provides an environmentally friendly and odorless mounting medium, comprising the following components in parts by mass: 15-20 parts of polyacrylate resin, 10-15 parts of dammar resin, 5-10 parts of fir resin, 3-5 parts of hydrogenated terpene resin, 2-5 parts of tributyl citrate, and 45-65 parts of n-decane.

[0007] Preferably, the polyacrylate resin comprises 80-85 wt% of n-octyl acrylate, 10-15 wt% of methyl methacrylate, and 5-10 wt% of hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0008] The third aspect of the present application provides a preparation method of the environmentally friendly and odorless mounting medium, comprising the following steps: S1. dispersing the dammar resin, the fir resin, the hydrogenated terpene resin, and the tributyl citrate in the n-decane to obtain a mixed solution; S2. adding n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate to the mixed solution, and then adding a initiator solution dropwise to perform a polymerization embedding reaction, thereby obtaining the environmentally friendly and odorless mounting medium.

[0009] Preferably, before step S1, the method further comprises the following step: purifying the dammar resin and the fir resin by recrystallization using a purification solvent, and then grinding and crushing.

[0010] Preferably, the initiator solution comprises a benzoyl peroxide solution in n-decane.

[0011] Preferably, the mass of the initiator is 0.3-0.5% of the total mass of the monomers (n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate).

[0012] Preferably, the purification solvent comprises a mixed solution of petroleum ether, ethyl acetate, and ethanol in a volume ratio of (2-3):(2-3):(3-6).

[0013] Preferably, the temperature for stirring and dispersing is 25-35°C, and the stirring and dispersing speed is 180-200 r / min.

[0014] Preferably, the temperature for the polymerization embedding reaction is 80-90°C, and the reaction time is 6-8 h.

[0015] The third aspect of the present application provides an application of the environmentally friendly and odorless mounting medium in the preparation of medical pathological sections. The beneficial effects of the present application are as follows: the present application is compounded by natural resin (dammar resin, fir resin, hydrogenated terpene resin) and synthetic resin (polyacrylate resin), and uses n-decane as a solvent, which not only effectively avoids the health risks and environmental pollution problems caused by the use of harmful solvents such as xylene in traditional mounting glue, realizes the non-toxic and odorless environmental protection characteristics of the product, but also improves the stability and adhesion of the mounting glue, enhances the optical transmittance and refractive index matching, and has fast drying performance, which is suitable for medical pathological sections. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical scheme and advantages of the present application clearer, the present application will be further described in detail below with examples. It should be understood that the specific examples described herein are only used to explain the present application and not to limit the present application.

[0017] The present application provides an environmentally friendly odorless mounting glue, which comprises the following components in mass fraction: 15-20 parts of polyacrylate resin, 10-15 parts of dammar resin, 5-10 parts of fir resin, 3-5 parts of hydrogenated terpene resin, 2-5 parts of tributyl citrate, and 45-65 parts of n-decane.

[0018] The natural resin (dammar resin, fir resin, hydrogenated terpene resin) used in the present application is compounded with synthetic resin (polyacrylate resin), which has better environmental protection and stability than using synthetic resin alone; the solvent used is n-decane, which is beneficial to the environmental protection of the mounting glue and improves its fast drying and non-foaming effect; the fir resin is used to improve the toughness of the mounting glue after drying and curing, and the hydrogenated terpene resin is used to adjust the viscosity of the mounting glue, which is convenient for coating, and achieves the effect of no glue flow, no glue leakage, initial adhesion of the glass slide, and no sliding; tributyl citrate, on the one hand, can effectively avoid the influence of resin crystallization on the transparency of the mounting glue during drying and curing, and is convenient for long-term storage; on the other hand, tributyl citrate can also speed up the dissolution rate of dammar resin in n-decane.

[0019] The mounting glue obtained by compounding the components is non-toxic, odorless, environmentally friendly, has excellent refractive index, fast drying, and strong adhesion.

[0020] In some embodiments, the environmentally friendly odorless mounting glue comprises the following components in mass fraction: 15 parts of polyacrylate resin, 10 parts of dammar resin, 6 parts of fir resin, 4 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, and 62 parts of n-decane.

[0021] In some embodiments, the environmentally friendly odorless mounting glue comprises the following components in mass fraction: 20 parts of polyacrylate resin, 12 parts of dammar resin, 8 parts of fir resin, 3 parts of hydrogenated terpene resin, 2 parts of tributyl citrate, and 55 parts of n-decane.

[0022] In some embodiments, the environment-friendly and odorless mounting medium comprises the following components in mass fraction: 18 parts of polyacrylate resin, 14 parts of dammar resin, 10 parts of fir resin, 5 parts of hydrogenated terpene resin, 5 parts of tributyl citrate, and 48 parts of n-decane.

[0023] In some embodiments, the polyacrylate resin comprises 80-85 wt% of n-octyl acrylate, 10-15 wt% of methyl methacrylate, and 5-10 wt% of hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0024] The polyacrylate resin of the present application is obtained by polymerization of n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate under the action of an initiator; preferably, the mass ratio of n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate is 80:15:5.

[0025] The polyacrylate resin of the present application is mainly composed of n-octyl acrylate monomer with a long alkyl chain, which reduces the rigidity and polarity of the molecular chain of the polyacrylate resin, increases its solubility in the environment-friendly solvent n-decane, and improves the initial adhesion of the composite glue. In addition, the functional monomer hydroxyethyl acrylate increases the bonding force between the polyacrylate resin and the natural resin, making the polyacrylate resin and the natural resin highly miscible, improving the adhesion of the dammar resin and the fir resin after drying, adjusting the refractive index, and increasing the observation clarity.

[0026] The present application provides a preparation method of an environment-friendly and odorless mounting medium, comprising the following steps: S1. Disperse dammar resin, fir resin, hydrogenated terpene resin, and tributyl citrate in n-decane to obtain a mixed solution; S2. Add n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate to the mixed solution, and then add an initiator solution dropwise to perform a polymerization embedding reaction, thereby obtaining the environment-friendly and odorless mounting medium.

[0027] The present application first dissolves the natural resin, monomer, and modifier to make them uniformly mixed into a homogeneous phase, and then adds an initiator to initiate the polymerization embedding of the monomer, thereby solving the problem that the polyacrylate resin is difficult to mix with dammar resin and fir resin in n-decane. The mounting medium prepared by the method has good miscibility, adjustable viscosity, can form a stable composite resin solution, and does not separate, precipitate, or discolor after long-term storage.

[0028] In some embodiments, before step S1, the method further comprises the step of: purifying and processing dammar resin and fir resin by recrystallization with a purification solvent, and then grinding and crushing.

[0029] In this embodiment, the purification treatment by mixed solvent recrystallization removes the insoluble impurities in the natural resin, facilitates the subsequent glue dissolution, improves the glue transparency, and reduces the color and luster; and the grinding and crushing are conducive to the rapid dissolution and solubility of the natural resin, and improve the dispersibility of the natural resin and the acrylate resin.

[0030] In some embodiments, the initiator solution comprises a n-decane solution of benzoyl peroxide.

[0031] In this embodiment, the volume ratio of n-decane for dissolving the initiator to n-decane in step S1 is 25-45:20.

[0032] In some embodiments, the mass of the initiator is 0.3%-0.5% of the total mass of the monomers (n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate).

[0033] In some embodiments, the purification solvent comprises a mixed solution of petroleum ether, ethyl acetate, and ethanol in a volume ratio of (2-3):(2-3):(3-6).

[0034] In some embodiments, the temperature for stirring and dispersing is 25-35℃, and the stirring and dispersing speed is 180-200r / min.

[0035] In some embodiments, the temperature for the polymerization embedding reaction is 80-90℃, and the reaction time is 6-8h.

[0036] In some embodiments, the specific operation of step S2 is: adding a compounded polymer monomer to the mixed solution, the compounded polymer monomer comprising n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate weighed in proportion, and then slowly adding the initiator solution at a uniform speed under 80-90℃, and continuing to react for 4-5h under 80-90℃ after the dropping is completed in 2-3h, to obtain the environmentally friendly odorless mounting medium.

[0037] The application provides an application of an environmentally friendly odorless mounting medium in medical pathological section preparation. The application is further described below through specific embodiments.

[0038] Embodiment 1 An environmentally friendly odorless mounting medium comprises the following components in mass fractions: 16 parts of polyacrylate resin, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, and 62 parts of n-decane; the polyacrylate resin comprises 80wt% n-octyl acrylate, 15wt% methyl methacrylate, and 5wt% hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0039] A preparation method of an environmentally friendly odorless mounting medium comprises the following steps: S1. The natural dammar resin raw material, natural fir resin raw material is recrystallized with mixed solvent (by volume ratio, petroleum ether: ethyl acetate: ethanol = 3:3:4) Purification treatment, remove insoluble impurities in natural resin, and then crushed into powder, get dammar resin and fir resin;Into a 500 mL three-necked flask, 42 parts of n-decane solvent, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, mechanical stirring at room temperature 25℃, speed 180 r / min, until completely dissolved, get mixed solution; S2. To the mixed solution, add 16 parts of compound polymer monomer (a mixture of n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate with a mass ratio of 85:15:5), stir evenly at room temperature, then continue to stir and heat to 80℃, slowly drop the n-decane solution of 0.4wt% benzoyl peroxide (n-decane usage is 20 parts) at 80℃, 2 hours drop, after the initiator is added, continue to react at 80℃ for 4 hours, so that the compound polymer monomer reaction is complete, cool to room temperature, get the environment-friendly odorless mounting medium.

[0040] Example 2 An environment-friendly odorless mounting medium, comprising the following components in mass fraction: 18 parts of polyacrylate resin, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, 60 parts of n-decane; the polyacrylate resin comprises 80wt% n-octyl acrylate, 15wt% methyl methacrylate and 5wt% hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0041] A preparation method of an environment-friendly odorless mounting medium, comprising the following steps: S1. The natural dammar resin raw material, natural fir resin raw material is recrystallized with mixed solvent (by volume ratio, petroleum ether: ethyl acetate: ethanol = 3:3:4) Purification treatment, remove insoluble impurities in natural resin, and then crushed into powder, get dammar resin and fir resin;Into a 500 mL three-necked flask, 42 parts of n-decane solvent, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, mechanical stirring at room temperature 25℃, speed 180 r / min, until completely dissolved, get mixed solution; S2. To the mixed solution, add 16 parts of compound polymer monomer (a mixture of n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate with a mass ratio of 85:15:5), stir evenly at room temperature, then continue to stir and heat to 80℃, slowly drop the n-decane solution of 0.4wt% benzoyl peroxide (n-decane usage is 20 parts) at 80℃, 2 hours drop, after the initiator is added, continue to react at 80℃ for 4 hours, so that the compound polymer monomer reaction is complete, cool to room temperature, get the environment-friendly odorless mounting medium.

[0042] Example 3 An environmentally friendly and odorless mounting adhesive, comprising components in mass fractions as follows: 20 parts of polyacrylate resin, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, 58 parts of n-decane; the polyacrylate resin comprises 80wt% n-octyl acrylate, 15wt% methyl methacrylate and 5wt% hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0043] A preparation method of an environmentally friendly and odorless mounting adhesive, comprising the following steps: S1. Recrystallize and purify natural dammar resin raw materials and natural fir resin raw materials with a mixed solvent (petroleum ether: ethyl acetate: ethanol = 3:3:4 by volume ratio) to remove insoluble impurities in the natural resin, and then crush into powder to obtain dammar resin and fir resin; add 38 parts of n-decane solvent, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, and 3 parts of tributyl citrate into a 500mL three-necked flask, and mechanically stir at a speed of 180 revolutions per minute at room temperature 25℃ until completely dissolved to obtain a mixed solution; S2. Add 20 parts of compounded polymer monomer (a mixture of n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate with a mass ratio of 85:15:5) to the mixed solution, stir uniformly at room temperature, and then continue to stir and heat to 80℃, slowly and uniformly drop 0.4wt% benzoyl peroxide n-decane solution (n-decane usage amount is 20 parts) at 80℃, drop for 2 hours, continue to react at 80℃ for 4 hours after the initiator is completely added, so that the compounded polymer monomer is completely reacted, and then cool to room temperature to obtain the environmentally friendly and odorless mounting adhesive.

[0044] Example 4 An environmentally friendly and odorless mounting adhesive, other contents are the same as example 1, the difference is that the compounded polymer monomer is a mixture of n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate with a mass ratio of 60:30:10.

[0045] Comparative example 1 A mounting adhesive, comprising components in mass fractions as follows: 16 parts of polyacrylate resin, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, 62 parts of xylene; the polyacrylate resin comprises 80wt% n-octyl acrylate, 15wt% methyl methacrylate and 5wt% hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0046] A preparation method of a mounting adhesive, comprising the following steps: S1. The natural dammar resin raw material and the natural fir resin raw material are purified by recrystallization with a mixed solvent (petroleum ether: ethyl acetate: ethanol = 3:3:4 by volume ratio) to remove insoluble impurities in the natural resin, and then are crushed into powder to obtain the dammar resin and the fir resin; 42 parts of xylene solvent, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, and 3 parts of tributyl citrate are added into a 500 mL three-necked flask, and are mechanically stirred at a speed of 180 revolutions per minute until completely dissolved at room temperature of 25℃ to obtain a mixed solution; S2. 16 parts of a compounded polymer monomer (a mixture of n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate with a mass ratio of 85:15:5) are added into the mixed solution, and are uniformly stirred at room temperature, and then are continuously stirred and heated to 80℃, and a xylene solution of benzoyl peroxide with a concentration of 0.4wt% (20 parts of xylene) is slowly and uniformly added dropwise at 80℃, and the dropping is completed in 2 hours, and after the initiator is completely added, the reaction is continued at 80℃ for 4 hours to completely react the compounded polymer monomer, and the reaction system is cooled to room temperature to obtain the environment-friendly and odorless mounting resin.

[0047] Comparative Example 2 A mounting resin comprises the following components in mass parts: 30 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, 3 parts of tributyl citrate, and 58 parts of n-decane.

[0048] A preparation method of a mounting resin comprises the following steps: The natural dammar resin raw material and the natural fir resin raw material are purified by recrystallization with a mixed solvent (petroleum ether: ethyl acetate: ethanol = 3:3:4 by volume ratio) to remove insoluble impurities in the natural resin, and then are crushed into powder to obtain the dammar resin and the fir resin; 42 parts of n-decane solvent, 10 parts of dammar resin, 6 parts of fir resin, 3 parts of hydrogenated terpene resin, and 3 parts of tributyl citrate are added into a 500 mL three-necked flask, and are mechanically stirred at a speed of 180 revolutions per minute until completely dissolved at room temperature of 25℃ to obtain a mixed solution, which is the mounting resin.

[0049] Comparative Example 3 A mounting resin comprises the following components in mass parts: 40 parts of polyacrylate resin and 60 parts of n-decane; the polyacrylate resin comprises 80wt% of n-octyl acrylate, 15wt% of methyl methacrylate, and 5wt% of hydroxyethyl acrylate, based on the total mass of the polyacrylate resin.

[0050] A preparation method of a mounting resin comprises the following steps: S1. 40 parts of n-decane solvent, 20 parts of complex polymerized monomer (a mixture of n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate with a mass ratio of 85:15:5) were added into a 500 mL three-necked flask, and stirred uniformly at a speed of 180 rpm to obtain a mixed solution; S2. The mixed solution was continuously stirred and heated to 80°C, and a 0.4wt% benzoyl peroxide n-decane solution (20 parts of n-decane were used) was slowly and uniformly added at 80°C. The dropping was completed in 2 hours. After the initiator was added, the reaction was continued at 80°C for 4 hours to make the complex polymerized monomer react completely, and then cooled to room temperature to obtain the mounting medium.

[0051] Comparative Example 4 A mounting medium, other contents are the same as Example 1, the difference is that the dammar resin is replaced by fir resin.

[0052] Comparative Example 5 A mounting medium, other contents are the same as Example 1, the difference is that the fir resin is replaced by dammar resin.

[0053] Comparative Example 6 A mounting medium, other contents are the same as Example 1, the difference is that the hydrogenated terpene resin is not included.

[0054] Comparative Example 7 A mounting medium, other contents are the same as Example 1, the difference is that the tributyl citrate is not included.

[0055] Test and evaluation The performance of the mounting medium was tested, and the specific content was as follows: 1. Initial adhesion test In a temperature of 25°C environment, the initial adhesion force was tested by using the tape initial adhesion force tester according to the national standard GB4852-2002 steel ball slope test method. The mounting medium was coated on a PET sheet with a width of 2 cm and a length of 10 cm (the glue layer was 20um thick), the tape was fixed on the inclined surface at an angle of 30°, and the steel balls with different diameters were placed at the front end of the glue 5 cm to make them freely roll. The number of steel balls remaining on the tape was recorded. The refractive index of the mounting medium and the cover glass is more similar, the better the clarity of the microscope image. The larger the ball number, the greater the initial adhesion 2. Adhesion test The holding adhesion force test was carried out according to GB4851-1998 suspension method at room temperature using a room temperature type adhesive tape holding force tester. The glue was made into a tape with a width of 2 cm and a length of 10 cm, which was adhered to the surface of a stainless steel plate and manually rolled back and forth three times using a roller. After standing for 20 min, it was hung on the tester and a 1000 g weight was hung below. The time for the pressure-sensitive adhesive tape to remain on the steel plate was recorded. The results are shown in Table 1.

[0056] The refractive index of the sample was measured using an Abbe refractometer. The instrument power was turned on, and the refractometer was calibrated using double-distilled water (the refractive index of distilled water at 20℃ is 1.3330), and the crosshair was adjusted to be clear. Sample testing: 1-2 drops of glue sample were dropped onto the prism surface (the sample was directly placed on the prism), the prism was closed, and the light source was adjusted so that the light-dark boundary was at the cross point. The refractive index value displayed by the instrument was recorded, and the average of three repeated measurements was taken. (Note: turbid samples cannot be measured).

[0057] Table 1 Test results

[0058] The environment-friendly and odorless mounting adhesive and its preparation method of the present application, by compounding natural resin (dammar resin, fir resin, hydrogenated terpene resin) with synthetic resin (polyacrylate resin) and using n-decane as a solvent, not only effectively avoids the health risks and environmental pollution problems caused by the use of harmful solvents such as xylene in traditional mounting adhesive, realizes the non-toxic and odorless environmental protection characteristics of the product, but also improves the stability and adhesion performance of the mounting adhesive. In particular, by optimizing the component ratio, the optical transmittance and refractive index matching of the mounting adhesive are enhanced, ensuring high definition during microscopic observation. The addition of tributyl citrate further prevents the decrease in transparency caused by resin crystallization, ensuring the long-term preservation quality of the sample. In addition, the addition of fir resin improves the toughness after drying and curing, and the hydrogenated terpene resin adjusts the viscosity of the mounting adhesive, making it easy to apply and not prone to glue overflow, with good initial adhesion and non-slip properties, especially suitable for machine automated mounting process. Overall, this new type of mounting adhesive not only meets the strict requirements of medical pathological sections on materials, but also solves many shortcomings in the prior art, exhibits excellent comprehensive performance, and provides safer and more efficient technical support for pathological research and clinical diagnosis.

[0059] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any changes or replacements within the technical scope disclosed by the present application can be easily thought of by those skilled in the art, and should be covered within the protection scope of the present application.​

Claims

1. An environmentally friendly odorless sealing adhesive, characterized in that: The invention comprises the following components in parts by mass: 15-20 parts of polyacrylate resin, 10-15 parts of dammar resin, 5-10 parts of fir resin, 3-5 parts of hydrogenated terpene resin, 2-5 parts of tributyl citrate and 45-65 parts of n-decane.

2. The environmentally friendly odorless sealing adhesive according to claim 1, characterized in that Based on the total mass of the polyacrylate resin, the polyacrylate resin includes 80-85 wt % of n-octyl acrylate, 10-15 wt % of methyl methacrylate, and 5-10 wt % of hydroxyethyl acrylate.

3. A method for preparing the environmentally friendly odorless sealing adhesive according to claim 1, characterized in that: The following steps are involved: S1. Damar resin, fir resin, hydrogenated terpene resin, and tributyl citrate were stirred and dispersed in n-decane to obtain a mixed solution; S2. Add n-octyl acrylate, methyl methacrylate, and hydroxyethyl acrylate to the mixed solution, and then dropwise add an initiator solution to carry out a polymerization and embedding reaction to obtain the environmentally friendly and odorless sealing adhesive.

4. The method for preparing the environmentally friendly odorless sealing adhesive according to claim 2, characterized in that: Before step S1, the method further includes the steps of: using a purification solvent to recrystallize and purify the dammar resin and the fir resin, and then grinding and crushing them.

5. The method for preparing the environmentally friendly odorless sealing adhesive according to claim 2, characterized in that: The initiator solution includes a solution of benzoyl peroxide in n-decane.

6. The method for preparing the environmentally friendly odorless sealing adhesive according to claim 2, characterized in that: The mass of the initiator is 0.3%-0.5% of the total mass of the n-octyl acrylate, methyl methacrylate and hydroxyethyl acrylate.

7. The method for preparing the environmentally friendly odorless sealing adhesive according to claim 4, characterized in that: The purification solvent includes a mixed solution of petroleum ether, ethyl acetate, and ethanol in a volume ratio of (2-3):(2-3):(3-6).

8. The method for preparing the environmentally friendly odorless sealing adhesive according to claim 2, characterized in that: The stirring and dispersing temperature is 25-35° C., and the stirring and dispersing speed is 180-200 r / min.

9. The method for preparing the environmentally friendly odorless sealing adhesive according to claim 2, characterized in that: The temperature of the polymerization embedding reaction is 80-90° C., and the duration of the dropwise addition is 6-8 hours.

10. Use of the environmentally friendly and odorless sealing adhesive according to claim 1 in the preparation of medical pathological sections.

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