Method for forming rhizopus arrhizus biofilm

By optimizing the culture medium composition and culture conditions and using calcium chloride to promote the secretion of extracellular polymers, the problems of stability and insufficient nutrient supply of Rhizopus arrhizus biofilm in industrial production were solved, and the formation and stability of efficient biofilm were achieved, which is suitable for industrial fermentation, wastewater treatment and biodegradation.

CN120796078APending Publication Date: 2025-10-17THE 980TH HOSPITAL OF THE CHINESE PEOPLES LIBERATION ARMY JOINT LOGISTICS SUPPORT FORCE
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Patent Information

Application Number
CN202510922718.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-04
Publication Date
2025-10-17

AI Technical Summary

Technical Problem

In the existing technology, the method for forming Rhizopus arrhizus biofilm has problems of low stability, insufficient nutrient supply and insufficient dissolved oxygen in industrial production, which leads to the disintegration of the biofilm and is difficult to meet the needs of industrial production.

Method used

By preparing a culture medium with specific ingredients, controlling the culture temperature and dissolved oxygen concentration, and using calcium chloride as an inducer, the secretion of extracellular polymers is promoted to form a stable and efficient biofilm.

Benefits of technology

A stable biofilm is formed in a short period of time, which improves the thickness and stability of the biofilm, adapts to various industrial application scenarios, and meets the needs of industrial production.

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Abstract

The invention provides a method for forming a rhizopus arrhizus biological membrane, and aims to realize efficient and stable formation of the biological membrane by optimizing a culture medium formula and controlling culture conditions. The method comprises the following steps: preparing a culture medium with specific components, adding a carbon source, a nitrogen source, monopotassium phosphate, magnesium sulfate and various trace elements, and culturing by inoculating rhizopus arrhizus strains. In the culture process, the secretion of extracellular polymeric substances is promoted and the formation of biological membranes is accelerated by continuously supplying oxygen and adding a calcium chloride inducer. And culturing in a constant-temperature environment of 28-32 DEG C for 48-72 hours to obtain a stable rhizopus arrhizus biological membrane. Compared with the prior art, the culture method provided by the invention can effectively improve the thickness and stability of the biological membrane and shorten the formation period, and is suitable for industrial application requirements.
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