Whole intervertebral disc decellularized tissue engineering scaffold as well as preparation method and application thereof

By using whole-disc decellularization technology and biological factor guidance, intervertebral disc scaffolds with preserved physiological structures were prepared, solving the problems of overall intervertebral disc repair and interface healing, and achieving efficient intervertebral disc regeneration.

CN120860323APending Publication Date: 2025-10-31JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Application Number
CN202511273360.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-08
Publication Date
2025-10-31

AI Technical Summary

Technical Problem

Existing technologies struggle to achieve holistic repair of the intervertebral disc, and the survival of seed cells and interface healing are challenging under harsh pathological conditions. As a result, artificial intervertebral disc prostheses cannot fully replicate the structure and function of the intervertebral disc, and the cartilage-bone interface is difficult to heal.

Method used

Using whole intervertebral disc decellularization technology, a decellularized scaffold that retains the physiological and mechanical structure of the intervertebral disc is prepared through steps such as surface polishing, degreasing, cryopreservation, shaking, decellularization and nuclease treatment. Combined with osteogenic inducing factors and stem cells, the structural and functional gradient of the interface is simulated to achieve biointegration of the cartilage-bone interface.

Benefits of technology

It successfully removed 70% of the endogenous cells, while preserving the glycosaminoglycan content and collagen fiber structure of the intervertebral disc, providing suitable porosity, promoting cell seeding and proliferation, improving biocompatibility, and solving the problems of overall intervertebral disc repair and interface healing.

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Abstract

The invention relates to the technical field of biological tissue engineering scaffolds, in particular to a whole intervertebral disc decellularized tissue engineering scaffold as well as a preparation method and application thereof. The method comprises the following steps: taking an intervertebral disc-bone complex of a vertebrate as a sample, and sequentially carrying out surface polishing treatment, degreasing treatment and cryopreservation rewarming treatment on the sample to obtain a pretreated sample; placing the pretreated sample in a Tris-HCl buffer solution containing a surfactant and a protease inhibitor, and carrying out oscillation treatment; putting the sample subjected to oscillation treatment into a decellularization solution, and carrying out decellularization treatment; putting the sample subjected to the decellularization treatment into a nuclease buffer solution, and carrying out nuclease treatment; and sequentially washing and freeze-drying the nuclease treated sample to obtain the whole intervertebral disc decellularized tissue engineering scaffold. Most of cell components and genetic materials are removed from the scaffold, a relatively complete extracellular matrix structure and mechanical properties are reserved, and the scaffold can be applied to the field of intervertebral disc repair and regenerative medicine.
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