Nano-material-mediated plant root exudate regulation and control method and salt and alkali resistance application

Abstract

The invention relates to the technical field of plant root secretion, in particular to a nano-material-mediated plant root secretion regulation and control method and salt and alkali resistance application, and the method comprises the following steps: preparation of a modified nano-material agent: adding a modified nano-titanium agent into a carbon nano tube solution with the amount 3-5 times of the total amount of the modified nano-titanium agent, and uniformly stirring, and adding an intervention material accounting for 10-15% of the total amount of the carbon nanotube liquid, and uniformly stirring to obtain the modified nano material agent. According to the nano-material mediated plant root exudate regulation and control method, the modified nano-material agent is prepared by matching the modified nano-material agent with the carbon nano-tube liquid and the intervention material, and meanwhile, soil mixing is adopted for regulation and control treatment; the prepared modified nano titanium agent can better regulate and control plant root exudates in a system.

Description

Technical Field

[0001] This invention relates to the field of plant root secretion technology, specifically to a method for regulating plant root secretions mediated by nanomaterials and its application in salt and alkali resistance. Background Technology

[0002] With the increasing severity of global soil salinization and the continuous expansion of saline-alkali land, agricultural production faces a significant threat. Under salt-alkali stress, plant growth is inhibited, manifesting as reduced germination rates, slow growth, and decreased yields. Plant root exudates are crucial mediators for material exchange and signal transduction between plants and their rhizosphere environment; changes in their composition and content can influence plant responses to salt-alkali stress. However, under natural conditions, plant root exudates are often insufficient to effectively alleviate the damage caused by salt-alkali stress. Existing methods for regulating plant root exudates are simple, have poor salt-alkali resistance, and offer limited improvement in plant growth quality, thus restricting the efficiency of these methods. Summary of the Invention

[0003] In view of the shortcomings of the prior art, the purpose of this invention is to provide a method for regulating plant root exudates mediated by nanomaterials and its application in salt and alkali resistance, so as to solve the problems mentioned in the background art.

[0004] The present invention solves the technical problem by adopting the following technical solution:

[0005] This invention provides a method for regulating plant root exudates mediated by nanomaterials, comprising the following steps:

[0006] Step 1: Preparation of modified nanomaterial agents:

[0007] Add the modified nano-titanium agent to a carbon nanotube liquid with a volume of 3-5 times the total volume of the modified nano-titanium agent and stir until homogeneous. Then add an intervening material with a volume of 10-15% of the total volume of the carbon nanotube liquid and stir until homogeneous to obtain the modified nanomaterial agent.

[0008] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 50-100 mg / kg, and sow cotton seeds in soil with a salt content of 0.5-0.8% and a pH value of 9.0-9.5 to complete the treatment of plant root exudate regulation.

[0009] Preferably, the modified nano-titanium agent is prepared by:

[0010] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0011] S2: Mix 5-8 parts of sodium lignosulfonate solution, 2-4 parts of phosphate buffer solution and 2-3 parts of calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0012] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1000-1500 r / min for 2 hours. After the ball milling was completed, the modified liquid was obtained.

[0013] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0014] Preferably, the sintering temperature for the sintering treatment is 350-400℃, and the sintering time is 1 hour.

[0015] Preferably, the pH of the phosphate buffer solution is 5.5-6.0; and the mass fraction of the sodium lignosulfonate solution is 5-8%.

[0016] Preferably, the stirring speed for the stirring modification treatment is 350-400 r / min, and the stirring time is 2 h; the ultrasonic power for the ultrasonic treatment is 350-400 W, and the ultrasonic treatment time is 1 h.

[0017] Preferably, the preparation method of the carbon nanotube liquid is as follows:

[0018] S11: Preheat carbon nanotubes at 55-60℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0019] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0020] Preferably, the silane coupling agent is silane coupling agent KH560.

[0021] Preferably, the interventional material is prepared by uniformly mixing 5-8 parts of sodium humate, 2-4 parts of urea, 3-5 parts of magnesium oxide, and 2-4 parts of ammonium bicarbonate to obtain the interventional material.

[0022] The present invention also provides an application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] This invention relates to a method for regulating plant root exudates mediated by nanomaterials. The modified nanomaterial agent is prepared by combining a modified nanomaterial agent with carbon nanotube liquid and an intervention material. Soil mixing is used for regulation. The modified nanomaterial agent, containing nano-carbon powder, shell powder, and nano-bentonite, undergoes sintering improvement and optimization using a modification liquid. Through the harmonization and optimization of the raw materials, the resulting modified nanomaterial agent better regulates plant root exudates within the system. The carbon nanotube liquid, containing preheated carbon nanotubes, is mixed with silane coupling agent KH560, ethanol solvent, and water, and further optimized with silane liquid. This results in a carbon nanotube liquid that better carries the intervention material. The intervention material, containing sodium humate, urea, magnesium oxide, and ammonium bicarbonate, is harmonized and optimized. Through the synergistic effect of the raw materials, they are better integrated into the system, thereby enhancing the regulation effect of plant root exudates. The product exhibits excellent salt and alkali resistance and significantly improves plant growth quality. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to specific examples. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] The nanomaterial-mediated method for regulating plant root exudates in this embodiment includes the following steps:

[0027] Step 1: Preparation of modified nanomaterial agents:

[0028] Add the modified nano-titanium agent to a carbon nanotube liquid with a volume of 3-5 times the total volume of the modified nano-titanium agent and stir until homogeneous. Then add an intervening material with a volume of 10-15% of the total volume of the carbon nanotube liquid and stir until homogeneous to obtain the modified nanomaterial agent.

[0029] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 50-100 mg / kg, and sow cotton seeds in soil with a salt content of 0.5-0.8% and a pH value of 9.0-9.5 to complete the treatment of plant root exudate regulation.

[0030] The preparation method of the modified nano-titanium agent in this embodiment is as follows:

[0031] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0032] S2: Mix 5-8 parts of sodium lignosulfonate solution, 2-4 parts of phosphate buffer solution and 2-3 parts of calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0033] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1000-1500 r / min for 2 hours. After the ball milling was completed, the modified liquid was obtained.

[0034] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0035] In this embodiment, the sintering temperature is 350-400℃, and the sintering time is 1 hour.

[0036] The pH of the phosphate buffer solution in this embodiment is 5.5-6.0; the mass fraction of the sodium lignosulfonate solution is 5-8%.

[0037] In this embodiment, the stirring speed for the stirring modification treatment is 350-400 r / min, and the stirring time is 2 h; the ultrasonic power for the ultrasonic treatment is 350-400 W, and the ultrasonic treatment time is 1 h.

[0038] The preparation method of the carbon nanotube liquid in this embodiment is as follows:

[0039] S11: Preheat carbon nanotubes at 55-60℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0040] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0041] The silane coupling agent in this embodiment is silane coupling agent KH560.

[0042] The preparation method of the interventional material in this embodiment is as follows: 5-8 parts of sodium humate, 2-4 parts of urea, 3-5 parts of magnesium oxide, and 2-4 parts of ammonium bicarbonate are mixed evenly to obtain the interventional material.

[0043] This embodiment describes the application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0044] Example 1

[0045] The nanomaterial-mediated method for regulating plant root exudates in this embodiment includes the following steps:

[0046] Step 1: Preparation of modified nanomaterial agents:

[0047] The modified nano-titanium agent was added to a carbon nanotube liquid with a volume of 3 times the total volume of the modified nano-titanium agent and stirred evenly. Then, an intervening material of 10% of the total volume of the carbon nanotube liquid was added and stirred evenly to obtain the modified nanomaterial agent.

[0048] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 50 mg / kg, and sow cotton seeds in soil with a salt content of 0.5% and a pH of 9.0 to complete the treatment of regulating plant root exudates.

[0049] The preparation method of the modified nano-titanium agent in this embodiment is as follows:

[0050] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0051] S2: Mix 5 parts sodium lignosulfonate solution, 2 parts phosphate buffer solution and 2 parts calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0052] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1000 r / min for 2 hours. After the ball milling was completed, the modified fluid was obtained.

[0053] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0054] In this embodiment, the sintering temperature is 350℃ and the sintering time is 1 hour.

[0055] The pH of the phosphate buffer solution in this embodiment is 5.5-6.0; the mass fraction of the sodium lignosulfonate solution is 5%.

[0056] In this embodiment, the stirring speed for the stirring modification treatment was 350 r / min, and the stirring time was 2 h; the ultrasonic power for the ultrasonic treatment was 350 W, and the ultrasonic treatment time was 1 h.

[0057] The preparation method of the carbon nanotube liquid in this embodiment is as follows:

[0058] S11: Preheat carbon nanotubes at 55℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0059] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0060] The silane coupling agent in this embodiment is silane coupling agent KH560.

[0061] The preparation method of the interventional material in this embodiment is as follows: 5 parts sodium humate, 2 parts urea, 3 parts magnesium oxide and 2 parts ammonium bicarbonate are mixed evenly to obtain the interventional material.

[0062] This embodiment describes the application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0063] Example 2

[0064] The nanomaterial-mediated method for regulating plant root exudates in this embodiment includes the following steps:

[0065] Step 1: Preparation of modified nanomaterial agents:

[0066] The modified nano-titanium agent was added to a carbon nanotube liquid with a volume of 5 times that of the modified nano-titanium agent and stirred evenly. Then, an intervening material with a volume of 15% of the carbon nanotube liquid was added and stirred evenly to obtain the modified nanomaterial agent.

[0067] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 100 mg / kg, and sow cotton seeds in soil with a salt content of 0.8% and a pH of 9.5 to complete the treatment of plant root exudate regulation.

[0068] The preparation method of the modified nano-titanium agent in this embodiment is as follows:

[0069] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0070] S2: Mix 8 parts of sodium lignosulfonate solution, 4 parts of phosphate buffer solution and 3 parts of calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0071] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1500 r / min for 2 hours. After the ball milling was completed, the modified fluid was obtained.

[0072] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0073] In this embodiment, the sintering temperature is 400℃ and the sintering time is 1 hour.

[0074] The pH of the phosphate buffer solution in this embodiment is 6.0; the mass fraction of the sodium lignosulfonate solution is 8%.

[0075] In this embodiment, the stirring speed for the stirring modification treatment was 400 r / min, and the stirring time was 2 h; the ultrasonic power for the ultrasonic treatment was 400 W, and the ultrasonic treatment time was 1 h.

[0076] The preparation method of the carbon nanotube liquid in this embodiment is as follows:

[0077] S11: Preheat carbon nanotubes at 60℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0078] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0079] The silane coupling agent in this embodiment is silane coupling agent KH560.

[0080] The preparation method of the interventional material in this embodiment is as follows: 8 parts sodium humate, 4 parts urea, 5 parts magnesium oxide and 4 parts ammonium bicarbonate are mixed evenly to obtain the interventional material.

[0081] This embodiment describes the application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0082] Example 3

[0083] The nanomaterial-mediated method for regulating plant root exudates in this embodiment includes the following steps:

[0084] Step 1: Preparation of modified nanomaterial agents:

[0085] The modified nano-titanium agent was added to a carbon nanotube liquid with a volume of 4 times the total volume of the modified nano-titanium agent and stirred evenly. Then, an intervening material of 12.5% ​​of the total volume of the carbon nanotube liquid was added and stirred evenly to obtain the modified nanomaterial agent.

[0086] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 75 mg / kg, and sow cotton seeds in soil with a salt content of 0.65% and a pH of 9.2 to complete the treatment of regulating plant root exudates.

[0087] The preparation method of the modified nano-titanium agent in this embodiment is as follows:

[0088] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0089] S2: Mix 6.5 parts of sodium lignosulfonate solution, 3 parts of phosphate buffer solution and 2.5 parts of calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0090] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1250 r / min for 2 hours. After the ball milling was completed, the modified liquid was obtained.

[0091] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0092] In this embodiment, the sintering temperature is 375℃ and the sintering time is 1 hour.

[0093] The pH of the phosphate buffer solution in this embodiment is 5.7; the mass fraction of the sodium lignosulfonate solution is 6.5%.

[0094] In this embodiment, the stirring speed for the stirring modification treatment was 375 r / min, and the stirring time was 2 h; the ultrasonic power for the ultrasonic treatment was 375 W, and the ultrasonic treatment time was 1 h.

[0095] The preparation method of the carbon nanotube liquid in this embodiment is as follows:

[0096] S11: Preheat carbon nanotubes at 57°C for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0097] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0098] The silane coupling agent in this embodiment is silane coupling agent KH560.

[0099] The preparation method of the interventional material in this embodiment is as follows: 6.5 parts of sodium humate, 3 parts of urea, 4 parts of magnesium oxide and 3 parts of ammonium bicarbonate are mixed evenly to obtain the interventional material.

[0100] This embodiment describes the application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0101] Example 4

[0102] The nanomaterial-mediated method for regulating plant root exudates in this embodiment includes the following steps:

[0103] Step 1: Preparation of modified nanomaterial agents:

[0104] The modified nano-titanium agent was added to a carbon nanotube liquid with a volume of 4 times the total volume of the modified nano-titanium agent and stirred evenly. Then, an intervening material of 12% of the total volume of the carbon nanotube liquid was added and stirred evenly to obtain the modified nanomaterial agent.

[0105] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 60 mg / kg, and sow cotton seeds in soil with a salt content of 0.6% and a pH of 9.2 to complete the treatment of plant root exudate regulation.

[0106] The preparation method of the modified nano-titanium agent in this embodiment is as follows:

[0107] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0108] S2: Mix 6 parts sodium lignosulfonate solution, 3 parts phosphate buffer solution and 2 parts calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0109] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1220 r / min for 2 hours. After the ball milling was completed, the modified liquid was obtained.

[0110] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0111] In this embodiment, the sintering temperature is 365°C and the sintering time is 1 hour.

[0112] The pH of the phosphate buffer solution in this embodiment is 5.6; the mass fraction of the sodium lignosulfonate solution is 6%.

[0113] In this embodiment, the stirring speed for the stirring modification treatment was 36 r / min, and the stirring time was 2 h; the ultrasonic power for the ultrasonic treatment was 370 W, and the ultrasonic treatment time was 1 h.

[0114] The preparation method of the carbon nanotube liquid in this embodiment is as follows:

[0115] S11: Preheat carbon nanotubes at 56℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0116] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0117] The silane coupling agent in this embodiment is silane coupling agent KH560.

[0118] The preparation method of the interventional material in this embodiment is as follows: 6 parts sodium humate, 3 parts urea, 4 parts magnesium oxide and 3 parts ammonium bicarbonate are mixed evenly to obtain the interventional material.

[0119] This embodiment describes the application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0120] Example 5

[0121] The nanomaterial-mediated method for regulating plant root exudates in this embodiment includes the following steps:

[0122] Step 1: Preparation of modified nanomaterial agents:

[0123] The modified nano-titanium agent was added to a carbon nanotube liquid with a volume of 4 times the total volume of the modified nano-titanium agent and stirred evenly. Then, an intervening material of 14% of the total volume of the carbon nanotube liquid was added and stirred evenly to obtain the modified nanomaterial agent.

[0124] Step 2: Mix the modified nanomaterial agent with the soil at a rate of 90 mg / kg, and sow cotton seeds in soil with a salt content of 0.7% and a pH of 9.2 to complete the treatment of regulating plant root exudates.

[0125] The preparation method of the modified nano-titanium agent in this embodiment is as follows:

[0126] S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained.

[0127] S2: Mix 7 parts sodium lignosulfonate solution, 3 parts phosphate buffer solution and 3 parts calcium sulfate whiskers evenly to obtain a modified liquid; stir the composite sintered body and the modified liquid at a weight ratio of 3:5 for modification treatment, and after stirring, obtain a ball milling liquid;

[0128] The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1450 r / min for 2 hours. After the ball milling was completed, the modified fluid was obtained.

[0129] S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

[0130] In this embodiment, the sintering temperature is 380℃ and the sintering time is 1 hour.

[0131] The pH of the phosphate buffer solution in this embodiment is 5.9; the mass fraction of the sodium lignosulfonate solution is 7%.

[0132] In this embodiment, the stirring speed for the stirring modification treatment was 390 r / min, and the stirring time was 2 h; the ultrasonic power for the ultrasonic treatment was 395 W, and the ultrasonic treatment time was 1 h.

[0133] The preparation method of the carbon nanotube liquid in this embodiment is as follows:

[0134] S11: Preheat carbon nanotubes at 58℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution.

[0135] S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

[0136] The silane coupling agent in this embodiment is silane coupling agent KH560.

[0137] The preparation method of the interventional material in this embodiment is as follows: 7 parts sodium humate, 3 parts urea, 4 parts magnesium oxide and 3 parts ammonium bicarbonate are mixed evenly to obtain the interventional material.

[0138] This embodiment describes the application of a nanomaterial-mediated method for regulating plant root exudates in salt and alkali resistance.

[0139] Comparative Example 1.

[0140] Unlike Example 3, no modified nano-titanium agent was added.

[0141] Comparative Example 2.

[0142] Unlike Example 3, no composite sintered body was added in the preparation of the modified nano-titanium agent.

[0143] Comparative Example 3.

[0144] Unlike Example 3, no modifying liquid was added during the preparation of the modified nano-titanium agent.

[0145] Comparative Example 4.

[0146] Unlike Example 3, no carbon nanotube liquid was added.

[0147] The processes of Examples 1-5 and Comparative Examples 1-6 were subjected to performance tests, and the test results are as follows.

[0148]

[0149]

[0150] Examples 1-5 and Comparative Examples 1-4 show that the process of the present invention can improve the growth quality of plants, and the process still has excellent salt and alkali resistance stability under saline and alkali conditions. The performance of the products without the addition of modified nano-titanium agent or carbon nanotube liquid is significantly worse. In addition, the performance of the products also tends to deteriorate when the composite sintered body and the modified liquid are not added in the preparation of modified nano-titanium agent. Only the process raw materials obtained by the method of the present invention have the most significant performance effect.

[0151] The above provides a detailed description of the nanomaterial-mediated regulation method for plant root exudates and its salt-alkali resistance application provided in this application. Specific examples have been used to illustrate the principles and implementation methods of this application. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this application. At the same time, for those skilled in the art, based on the ideas of this application, other variations can be made on the basis of the above description. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A method for regulating plant root exudates mediated by nanomaterials, characterized in that, Includes the following steps: Step 1: Preparation of modified nanomaterial agents: Add the modified nano-titanium agent to a carbon nanotube liquid with a volume of 3-5 times the total volume of the modified nano-titanium agent and stir until homogeneous. Then add an intervening material with a volume of 10-15% of the total volume of the carbon nanotube liquid and stir until homogeneous to obtain the modified nanomaterial agent. Step 2: Mix the modified nanomaterial agent with the soil at a rate of 50-100 mg / kg, and sow cotton seeds in soil with a salt content of 0.5-0.8% and a pH value of 9.0-9.5 to complete the treatment of plant root exudate regulation.

2. The method for regulating plant root exudates mediated by nanomaterials according to claim 1, characterized in that, The preparation method of the modified nano-titanium agent is as follows: S1: Nano carbon powder, shell powder and nano bentonite are mixed evenly in a weight ratio of 3:2:2, and then sintered. After sintering, a composite sintered body is obtained. S2: Mix 5-8 parts of sodium lignosulfonate solution, 2-4 parts of phosphate buffer solution and 2-3 parts of calcium sulfate whiskers evenly to obtain the modified solution; The composite sintered body and the modified liquid were stirred and modified at a weight ratio of 3:

5. After stirring, the ball milling liquid was obtained. The composite sintered body and the ball milling fluid were mixed at a weight ratio of 7:5 and ball milled at a speed of 1000-1500 r / min for 2 hours. After the ball milling was completed, the modified liquid was obtained. S3: Nano-titanium oxide and modified liquid are ultrasonically treated at a weight ratio of 6:

9. After stirring, the mixture is filtered and dried to obtain the modified nano-titanium oxide agent.

3. The method for regulating plant root exudates mediated by nanomaterials according to claim 2, characterized in that, The sintering temperature for the sintering treatment is 350-400℃, and the sintering time is 1 hour.

4. The method for regulating plant root exudates mediated by nanomaterials according to claim 2, characterized in that, The pH of the phosphate buffer solution is 5.5-6.0; the mass fraction of the sodium lignosulfonate solution is 5-8%.

5. The method for regulating plant root exudates mediated by nanomaterials according to claim 2, characterized in that, The stirring speed for the stirring modification treatment was 350-400 r / min, and the stirring time was 2 h; the ultrasonic power for the ultrasonic treatment was 350-400 W, and the ultrasonic treatment time was 1 h.

6. The method for regulating plant root exudates mediated by nanomaterials according to claim 1, characterized in that, The preparation method of carbon nanotube liquid is as follows: S11: Preheat carbon nanotubes at 55-60℃ for 1 hour to obtain preheated carbon nanotubes; mix silane coupling agent KH560, ethanol solvent and water in a weight ratio of 3:7:1 to obtain silane solution. S12: The preheated carbon nanotubes and silane liquid are stirred thoroughly at a weight ratio of 5:8 to obtain a carbon nanotube liquid.

7. The method for regulating plant root exudates mediated by nanomaterials according to claim 6, characterized in that, The silane coupling agent is silane coupling agent KH560.

8. The method for regulating plant root exudates mediated by nanomaterials according to claim 1, characterized in that, The preparation method of the interventional material is as follows: 5-8 parts of sodium humate, 2-4 parts of urea, 3-5 parts of magnesium oxide and 2-4 parts of ammonium bicarbonate are mixed evenly to obtain the interventional material.

9. The application of the nanomaterial-mediated plant root exudate regulation method as described in any one of claims 1-8 in salt and alkali resistance.

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