Composition with whitening effect and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a composition with a whitening effect and application thereof, and the composition is prepared from the following components in parts by weight: 0.005-1 part of tricholoma matsutake polysaccharide, 0.1-5 parts of panthenol and 1-12 parts of a whitening agent. The whitening agent is one or more of nicotinamide, tranexamic acid, ascorbic acid glucoside, undecylenoyl phenylalanine, phenylethyl resorcinol and arbutin. The whitening composition provided by the invention is not added with violating hormone components, richer intracellular and extracellular polysaccharides can be extracted from the tricholoma matsutake polysaccharides in a composite extraction mode (enzyme extraction and water extraction), the tricholoma matsutake polysaccharides with the molecular weight of 3KDa-60KDa are prepared after impurity removal and degradation treatment, and by combining panthenol and a whitening agent, the whitening effect is improved, and the whitening effect is improved. The synergistic effect shows unexpected whitening effect enhancement, the whitening speed is increased, and the composition is mild and non-irritant after being used.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a whitening composition and its application. Background Technology

[0002] Currently, some skin whitening products are slow to show results and have poor effects. This is because skin whitening is a complex physiological process, and using only a single whitening ingredient often cannot address all aspects. Therefore, some commercially available products may illegally add hormones to achieve good and rapid whitening effects. Although these products can bring significant whitening effects, long-term use may damage the skin barrier, and once discontinued, they can easily cause hormone-dependent dermatitis such as redness, swelling, itching, and peeling due to external stimuli.

[0003] Matsutake mushrooms, hailed as the king of mushrooms, are a natural delicacy rich in nutrients and possess significant medicinal value. They can promote skin cell regeneration and repair, helping to repair damaged skin barriers; participate in skin renewal, accelerate the metabolism of aging cells, promote collagen synthesis, and maintain skin elasticity and structure.

[0004] Panthenol, also known as provitamin B5, is a precursor to vitamin B5. It can effectively relieve symptoms such as skin erythema and itching, while also repairing the skin barrier and resisting external stimuli. Summary of the Invention

[0005] The purpose of this invention is to provide a whitening composition without the addition of illegal hormones and its application.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] This invention proposes a whitening composition comprising the following components in parts by weight: 0.005-1 parts of matsutake mushroom polysaccharide, 0.1-5 parts of panthenol, and 1-12 parts of whitening agent;

[0008] The whitening agent is one or more of nicotinamide, tranexamic acid, ascorbate glucoside, undecenoyl phenylalanine, phenylethyl resorcinol, and arbutin.

[0009] Furthermore, it is composed of the following components in parts by weight: 0.5 parts of matsutake mushroom polysaccharide, 1 part of panthenol, and 5 parts of whitening agent.

[0010] Furthermore, the molecular weight of the *Tricholoma matsutake* polysaccharide is 3 kDa-60 kDa.

[0011] Furthermore, the *Tricholoma matsutake* polysaccharide is prepared by the following steps:

[0012] S1, using cellulase to extract from pulverized matsutake mushroom powder, wherein the concentration of cellulase is 0.5%-5%, the reaction time is 30 min-150 min, and the reaction temperature is 30℃-70℃, to obtain a crude extract of matsutake mushroom.

[0013] S2, cellulase was used to extract the crude extract of Tricholoma matsutake, which was then subjected to water extraction. The reaction time was 30 min-150 min and the reaction temperature was 30℃-90℃. After water extraction, crude polysaccharide of Tricholoma matsutake was obtained.

[0014] S3, after removing impurities and degrading the prepared Tricholoma matsutake crude polysaccharide, Tricholoma matsutake polysaccharide with a molecular weight of 3KDa-60KDa was obtained.

[0015] Furthermore, in step S1, the cellulase concentration is 1.5%, the reaction time is 60 min, and the reaction temperature is 45°C.

[0016] Furthermore, the water extraction reaction time in step S2 is 90 min, and the reaction temperature is 80 °C.

[0017] Furthermore, the impurity removal and degradation treatment in step S3 includes one or more of the following: enzymatic hydrolysis, salting out, alcohol precipitation, chemical treatment, and membrane treatment.

[0018] The present invention also includes the use of any of the above-described whitening compositions in the preparation of cosmetics.

[0019] Furthermore, the cosmetic product is an aqueous solution, lotion, serum, face cream, eye cream, or mask.

[0020] Compared with existing technologies, this invention provides a highly effective whitening composition without the addition of illegal hormones. The matsutake mushroom polysaccharide of this invention can be extracted through a compound extraction method (enzyme extraction + water extraction) to obtain more abundant intracellular and extracellular polysaccharides. After impurity removal and degradation treatment, matsutake mushroom polysaccharides with a molecular weight of 3KDa-60KDa are obtained. Combined with panthenol and whitening agents, it exhibits an unexpectedly enhanced whitening effect, improves the speed of whitening, and is gentle and non-irritating after use. Detailed Implementation

[0021] The technical solution of the present invention will be further described below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanatory of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0022] Unless otherwise specified, all reagents involved in the embodiments of this invention are commercially available products.

[0023] Example 1

[0024] Prepare matsutake mushroom extract according to the following steps:

[0025] First, the powdered matsutake mushroom was extracted using cellulase at a concentration of 1.5%, a reaction time of 60 min, and a reaction temperature of 45℃ to obtain a crude extract of matsutake mushroom.

[0026] Secondly, the crude extract of *Tricholoma matsutake* obtained by cellulase extraction was then subjected to water extraction for 90 minutes at a temperature of 80°C. After water extraction, crude polysaccharide of *Tricholoma matsutake* with an average molecular weight of 400 kDa was obtained.

[0027] Finally, the prepared Tricholoma matsutake crude polysaccharide was hydrolyzed with 0.5% papain for 90 min at 45 °C to obtain Tricholoma matsutake polysaccharide with an average molecular weight of 10.8 kDa.

[0028] The test samples for Examples 2-5 below were prepared by adding water to each group in Table 1 to form a total of 100 parts of aqueous solution. Among them, the matsutake mushroom polysaccharide used in Examples 1-3 and Comparative Examples 4-15 was the matsutake mushroom polysaccharide with an average molecular weight of 10.8 kDa obtained above; the matsutake mushroom polysaccharide used in Comparative Example 16 was the matsutake mushroom crude polysaccharide with an average molecular weight of 400 kDa obtained after water extraction above.

[0029] Table 1. Components and weight parts of each group of compositions

[0030]

[0031] Example 2

[0032] Whitening efficacy evaluation

[0033] Tyrosinase is a key enzyme in the biosynthesis of melanin in the skin, determining not only the rate of melanin synthesis but also serving as a characteristic marker of melanocyte differentiation and maturation. In the process of melanin biosynthesis, tyrosine is hydroxylated to produce dopa, dopa is oxidized to produce dopaquinone, and dihydroxyindole is converted to indolequinone; all these reaction steps require the participation of tyrosinase. This experimental design used L-tyrosine as a substrate and adjusted the reaction times of L-tyrosine, tyrosinase, and the sample to determine the inhibitory effect and rate of the sample on in vitro tyrosinase activity, thus verifying the whitening efficacy of the composition. The inhibition rates of tyrosinase activity in each group are shown in Table 2 below.

[0034] Table 2 Tyrosinase Inhibition Rate

[0035]

[0036] Example 3

[0037] Repair efficacy evaluation

[0038] Tight junction protein (ZO-1) is a protein located beneath the cell membrane. Together with other tight junction proteins, it forms the structure of intercellular connections. The main function of ZO-1 is to maintain the stability and integrity of these connections, thereby preserving cell morphology and function. This experiment established a chemical damage model of keratinocytes (HaCaT cells) induced by SDS. The repair efficacy of the active ingredient was verified by detecting changes in tight junction protein (ZO-1) gene expression. The rate of change in ZO-1 gene expression in each group is shown in Table 3 below.

[0039] Table 3 Results of changes in tight junction protein expression rates

[0040] Group ZO-1 gene expression change rate Example 1 102.6％ Example 2 110.7％ Example 3 180.3％ Comparative Example 4 28.2％ Comparative Example 5 33.5％ Comparative Example 6 20.1％ Comparative Example 7 21.6％ Comparative Example 8 0 Comparative Example 9 9.1％ Comparative Example 10 0 Comparative Example 11 21.7％ Comparative Example 12 66.3％ Comparative Example 13 58.2％ Comparative Example 14 43.8％ Comparative Example 15 36.9％ Comparative Example 16 36.7％

[0041] Example 4

[0042] Firming effect evaluation

[0043] Type IV collagen is distributed on the basement membrane at the dermal-epidermal junction, and increased levels can improve the loosening of the dermal-epidermal junction caused by skin aging. This experiment established a chronic fibroblast injury model using UVA irradiation and verified the firming effect of the composition by detecting changes in the expression of the type IV collagen α1 chain gene (COL4A1). The COL4A1 expression change rates in each group are shown in Table 4 below.

[0044] Table 4. Type IV Collagen α1 Chain Gene

[0045]

[0046]

[0047] Example 5

[0048] Permeability evaluation

[0049] The skin's "brick wall structure" is the first line of defense against external substances; substances can only exert their activity better after penetrating into the skin. This experiment used Bama miniature pigs as a test model, verifying the penetration effect of the active ingredient by measuring the diffusion percentage of the receiving liquid and the sample loading amount after diffusion. The diffusion rates of each group are shown in Table 5 below.

[0050] Table 5 Diffusion rate results

[0051]

[0052]

[0053] While specific embodiments of the present invention have been described above, those skilled in the art should understand that the specific embodiments described are merely illustrative and not intended to limit the scope of the invention. Modifications and variations made by those skilled in the art in accordance with the spirit of the invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A whitening composition, characterized in that: It is composed of the following components in parts by weight: 0.005-1 part of matsutake mushroom polysaccharide, 0.1-5 parts of panthenol, and 1-12 parts of whitening agent; The whitening agent is one or more of nicotinamide, tranexamic acid, ascorbate glucoside, undecenoyl phenylalanine, phenylethyl resorcinol, and arbutin.

2. The whitening composition according to claim 1, characterized in that: It consists of the following components in parts by weight: 0.5 parts of matsutake mushroom polysaccharide, 1 part of panthenol, and 5 parts of nicotinamide.

3. The whitening composition according to claim 1, characterized in that: The molecular weight of the *Tricholoma matsutake* polysaccharide is 3 kDa-60 kDa.

4. The whitening composition according to claim 3, characterized in that: The *Tricholoma matsutake* polysaccharide was prepared by the following steps: S1, using cellulase to extract from pulverized matsutake mushroom powder, wherein the concentration of cellulase is 0.5%-5%, the reaction time is 30 min-150 min, and the reaction temperature is 30℃-70℃, to obtain a crude extract of matsutake mushroom. S2, cellulase was used to extract the crude extract of Tricholoma matsutake, which was then subjected to water extraction. The reaction time was 30 min-150 min and the reaction temperature was 30℃-90℃. After water extraction, crude polysaccharide of Tricholoma matsutake was obtained. S3, after removing impurities and degrading the prepared Tricholoma matsutake crude polysaccharide, Tricholoma matsutake polysaccharide with a molecular weight of 3KDa-60KDa was obtained.

5. The whitening composition according to claim 4, characterized in that: In step S1, the cellulase concentration is 1.5%, the reaction time is 60 min, and the reaction temperature is 45℃.

6. The whitening composition according to claim 4, characterized in that: The water extraction reaction time in step S2 is 90 min, and the reaction temperature is 80℃.

7. The whitening composition according to claim 4, characterized in that: The impurity removal and degradation treatment in step S3 includes one or more of the following: enzymatic hydrolysis, salting out, alcohol precipitation, chemical treatment, and membrane treatment.

8. The use of the whitening composition according to any one of claims 1-7 in the preparation of cosmetics.

9. The application according to claim 8, characterized in that: The cosmetics mentioned are liquids, lotions, serums, face creams, eye creams, or face masks.

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