Construction method and application of recombinant yarrowia lipolytica with high hydroxytyrosol yield
By constructing a recombinant Yersinia lipolytica that produces high levels of hydroxytyrosol, expressing specific enzymes and genes, and genetically modifying them, the problems of low production efficiency and high cost in existing technologies have been solved, and efficient industrial production of hydroxytyrosol has been achieved.
Patent Information
- Application Number
- CN202511540394.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-27
- Publication Date
- 2026-01-23
AI Technical Summary
Existing technologies lack microorganisms that can efficiently produce hydroxytyrosol. Traditional methods rely on plant resources and are costly, while chemical synthesis is not suitable for industrial production. Saccharomyces cerevisiae and Escherichia coli have limitations.
A recombinant Yersinia lipolytica with high hydroxytyrosol production was constructed, expressing a two-component riboflavin-dependent monooxygenase oxidase and reductase, a DAHP synthase mutant, and a bifunctional branched acid mutase/prebenzoic acid dehydrogenase mutant. Genes were modified to express phosphoketonease, phosphoacetyltransferase, aromatic aldehyde synthase, and hemoglobin, while prebenzoic acid dehydratase and anthranilic acid synthase were knocked out. Growth-dependent promoters were used to control gene expression.
The efficient synthesis of hydroxytyrosol has been achieved, solving the problems of low production efficiency and high cost in existing technologies, and making it suitable for industrial production.