Extraction method for lymphangial endothelial cell cyst fluid of lymphangial malformation

By directly collecting and simplifying the cystic fluid from lymphatic malformations, the problems of low cell purity and poor cell viability in traditional methods are solved, providing a high-quality cell model for lymphatic malformation research.

CN122012391APending Publication Date: 2026-05-12WEST CHINA HOSPITAL SICHUAN UNIV
0 Cites 0 Cited by

Patent Information

Application Number
CN202610033787.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-12
Publication Date
2026-05-12

AI Technical Summary

Technical Problem

Existing technologies for treating lymphatic malformations present challenges in obtaining samples through surgical tissue removal, resulting in low cell purity and poor viability. Furthermore, the procedures are cumbersome, making it difficult to provide high-quality cell models.

Method used

By collecting cystic fluid from patients with lymphatic malformations, performing low-speed centrifugation, erythrocyte lysis, and cell culture, combined with immunofluorescence staining for identification, the operation steps are simplified, and cell purity and activity are improved.

Benefits of technology

This method enables the low-invasive, simple, and efficient acquisition of high-purity lymphatic endothelial cells, making them suitable for basic research and clinical translation, and improving cell culture success rate and purity.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a lymphatic endothelial cell cyst fluid extraction method for lymphatic deformity, and belongs to the technical field of biological cell extraction. Comprising the following steps: S1, sample collection: collecting 5-10 mL of cystic fluid of a patient with macrocystic lymphangial malformation, and placing the collected cystic fluid in a sterile container; s2, primary centrifugation: centrifuging at a low speed of 1000rpm for 10 minutes, and retaining cell precipitates; s3, red blood cell lysis: adding a red blood cell lysis solution, placing at 4 DEG C for 3-5 minutes, centrifuging, and washing and purifying with PBS (Phosphate Buffer Solution); s4, cell culture: resuspending the cells in a culture medium containing an endothelial growth factor replenishing liquid, culturing at 37 DEG C in 5% CO2, and changing the liquid for 24 hours to remove non-adherent cells; and S5, cell identification: carrying out immunofluorescence staining identification 48-72 hours after cell adherence. The method is simple, convenient and efficient to operate, does not need complex digestion, can enrich high-purity and high-activity lymphatic endothelial cells, provides a stable cell model for related pathogenesis research and drug screening, and has important scientific research and clinical value.
Need to check novelty before this filing date? Find Prior Art