A composition with the effect of tonifying qi and blood, a preparation method, application and related products

Abstract

The application discloses a composition with qi and blood tonifying effect, a preparation method, application and related products, and belongs to the technical field of traditional Chinese medicines. The technical problem to be solved is to provide a traditional Chinese medicine composition with better qi and blood tonifying effect. The technical solution is characterized in that the composition is prepared from 5-15 parts of angelica sinensis, 9-30 parts of astragalus root and 7.5-15 parts of reynoutria root. The angelica sinensis is a mixture of raw angelica sinensis and vinegar-fried angelica sinensis, the astragalus root is a mixture of raw astragalus root and honey-fried astragalus root, and the reynoutria root is a mixture of raw reynoutria root and prepared reynoutria root. Animal experiments show that the composition provided by the application can significantly improve the levels of RBC, HGB, HCT, WBC and PLT of blood deficiency mice, and improve the levels of EPO, G-CSF, M-CSF and sVCAM-1 in serum.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, and relates to a composition with qi and blood replenishing effects, its preparation method, application and related products. Background Technology

[0002] For understanding the technical content of this invention: Traditional Chinese medicine theory holds that "qi and blood share the same origin" and "qi generates blood, and blood carries qi." Insufficient qi and blood can lead to symptoms such as sallow complexion, fatigue, dizziness, and weakened immunity, especially common in postpartum recovery, the elderly with weak constitutions, and those undergoing chronic disease management. Rehmannia glutinosa, Angelica sinensis, and Astragalus membranaceus are a classic combination for tonifying qi and blood, first recorded in the *Synopsis of Prescriptions of the Golden Chamber*. Rehmannia glutinosa nourishes yin and replenishes blood, Angelica sinensis replenishes and invigorates blood, and Astragalus membranaceus tonifies qi and raises yang. The three work synergistically to achieve "dual replenishment of qi and blood," making it a commonly used compound herbal formula in the field of traditional Chinese medicine.

[0003] Modern pharmacological studies have confirmed that the combined effects of Rehmannia glutinosa, Astragalus membranaceus, and Angelica sinensis in replenishing qi and blood stem from the synergistic effects of multiple active ingredients: Astragaloside A, cycloastragaloyl alcohol, and other saponins and isoflavones in Astragalus membranaceus can promote hematopoietic stem cell proliferation and enhance immune function; ferulic acid in Angelica sinensis can improve blood circulation and increase iron utilization; and catalpol, Rehmannia glutinosa polysaccharides, and phenolic components in Rehmannia glutinosa can protect the bone marrow hematopoietic microenvironment and reduce oxidative stress damage. However, most of these active ingredients are highly lipid-soluble and have low solubility in water, resulting in low bioavailability. Furthermore, ferulic acid is easily destroyed by high-temperature heating, and catalpol is easily oxidized and reduced at room temperature, leading to low retention rates of active ingredients in traditional preparations, which severely limits their clinical application.

[0004] Relevant patent documents retrieved: This document, published in China (CN103355655A) on October 23, 2013, discloses a composition and its preparation method for improving nutritional anemia. The composition is prepared from Astragalus membranaceus, Angelica sinensis, Codonopsis pilosula, jujube, and Rehmannia glutinosa in a specific weight ratio. It can be formulated into any commonly used preparation, preferably an oral preparation. This composition has the function of improving nutritional anemia.

[0005] Relevant non-patent literature retrieved: The master's thesis from Shanxi University, titled "Study on the Material Basis of Rehmannia glutinosa's Efficacy Based on a Blood Deficiency Model" (by Zhou Jia), published in June 2023, discloses that Rehmannia glutinosa has blood-tonifying effects. Both raw and processed Rehmannia glutinosa can increase body weight, peripheral blood cell count, and improve bone marrow condition in mice. However, this thesis does not disclose the formulation of Rehmannia glutinosa, Astragalus membranaceus, and Angelica sinensis together, nor does it provide a traditional Chinese medicine combination with better compatibility.

[0006] The prior art represented by the aforementioned documents has at least the following unresolved technical problems or defects: To address issues of solubility, stability, and bioavailability, existing technologies often employ encapsulation of active ingredients using wall materials such as cyclodextrin, modified starch, and maltodextrin. However, these wall materials are not derived from the traditional Chinese medicine itself and merely function as carriers; the excipients themselves cannot synergistically enhance the effects of replenishing qi and blood. Furthermore, in traditional preparations, Rehmannia glutinosa, Angelica sinensis, and Astragalus membranaceus are decocted together, resulting in the simultaneous release and absorption of active ingredients after administration. In practice, astragaloside A needs to be slowly absorbed in the intestines for sustained hematopoiesis, while ferulic acid needs to be rapidly released to improve circulation. Existing technologies lack targeted design for this "sequential effect," preventing the precise implementation of the synergistic logic of "replenishing qi-generating blood-activating blood" through formulation technology. Summary of the Invention

[0007] The purpose of this invention is to provide: A composition with qi- and blood-tonifying effects, and related technologies, to address how to better tonify qi and generate blood, improve the levels of RBC (red blood cells), HGB (hemoglobin), and HCT (hematocrit) in the peripheral blood of blood-deficient mice, and improve the levels of EPO (erythropoietin), G-CSF (neutrophil colony-stimulating factor), M-CSF (macrophage colony-stimulating factor), and sVCAM (vascular cell adhesion molecule-1) in the serum of blood-deficient mice, or combinations thereof.

[0008] Terminology Explanation Unless otherwise defined, all technical terms in this document have the same meanings as commonly understood by one of ordinary skill in the art to which the subject matter of the claims pertains. Unless otherwise stated, all patents, patent inventions, and publications cited in this document are incorporated herein by reference in their entirety. If multiple definitions exist for terms in this document, the definitions in this chapter shall prevail.

[0009] It should be understood that the above brief description and the following detailed description are exemplary and for illustrative purposes only, and do not limit the subject matter of the invention in any way. In this invention, the singular is used in conjunction with the plural unless otherwise specifically stated. It should also be noted that, unless otherwise stated, the use of “or” or “or” means “and / or”. Furthermore, the use of the term “comprising” and other forms such as “including,” “containing,” and “contains” are not limiting.

[0010] Unless otherwise stated, conventional methods within the scope of the art, such as mixing and dissolving, shall be used.

[0011] Unless specifically defined herein, the use of all commercially available products herein employs standard techniques. For example, it may be carried out using the manufacturer's instructions for use with the kit, or in accordance with methods known in the art or the description of this invention. The techniques and methods described herein can generally be implemented according to conventional methods well known in the art, based on the descriptions in the various summary and more specific documents cited and discussed in this specification.

[0012] The terms “optional / arbitrary” or “optionally / arbitrarily” mean that the event or situation described below may or may not occur, including both the occurrence and non-occurrence of the event or situation.

[0013] According to the "Dictionary of Traditional Chinese Medicine" (Second Edition, compiled by Nanjing University of Traditional Chinese Medicine), the information of the Chinese medicinal materials used in this invention is as follows.

[0014] Astragalus:

Synonyms

[0015] [Origin] The roots of Astragalus mongholicus and Astragalus membranaceus, both belonging to the genus Astragalus in the legume family.

[0016]

Original Plant

[0017] 2. Astragalus membranaceus (Fisch.) Bunge.

[0018] [Harvesting and Processing] Harvest 2-3 years after sowing, in September-November or before the winter buds sprout in spring. Dig deep to prevent root breakage, cut off the rhizome, shake off the soil, and sun-dry until semi-dry. Pile up for 1-2 days and sun-dry again until completely dry. Trim off the lateral roots and fibrous roots, and tie them into small bundles to obtain raw Astragalus membranaceus.

[0019] [Medicinal Properties] Sweet, warm. Enters the lung and spleen meridians.

[0020]

Functions and Indications

[0021] [Preparation Method] 1. Raw Astragalus: Remove impurities from the raw material, wash it, soak it thoroughly, cut it into thick slices, and dry it.

[0022] 2. Honey-fried Astragalus: Dilute refined honey with an appropriate amount of boiling water, add astragalus slices and mix well. Let it sit for a while, then place it in a pot and heat over low heat until it turns a deep yellow color and is no longer sticky. Remove and let cool.

[0023] Angelica sinensis:

Synonyms

[0024] [Origin] The root of Angelica sinensis, a plant belonging to the genus Angelica in the family Apiaceae.

[0025] [Original plant] Angelica sinensis (Oliv.) Diels [A. polymorpha Maxim. var. sinensis Oliv.].

[0026] [Harvesting and Processing] Generally, it takes 2 years for the angelica to be harvested. The above-ground parts are cut off in early October, and the roots are dug up in late October. After the moisture has evaporated slightly, they are bundled, and a shed is built to dry them. First, they are smoked with wet firewood to color the angelica until the skin turns reddish-brown, and then they are dried with coal or firewood.

[0027] [Medicinal Properties] Sweet, pungent, bitter, warm. It enters the liver, heart, and spleen meridians.

[0028]

Functions and Indications

[0029] [Preparation Method] 1. Angelica sinensis: Take the raw medicinal material, remove impurities, wash it, slice it thinly, and sun-dry or dry it at low temperature.

[0030] 2. Vinegar-processed Angelica: Slice the Angelica. Add 10-20g of rice vinegar to 100g of Angelica slices, mix thoroughly, and let it soak until the herb softens and has no dry core. Pour the soaked Angelica slices into a hot pan, stir-fry quickly until the surface of the Angelica turns dark yellow (or old yellow), with slight charred spots. When removed, a strong aroma of vinegar should be present. Quickly remove the fried Angelica from the pan and place it on a clean bamboo tray or drying rack. Spread it thinly and let it cool quickly.

[0031] Rehmannia glutinosa

Synonyms

[0032]

Origin

[0033] [Original plant] Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch.

[0034] [Harvesting and Processing] Early-harvested Rehmannia glutinosa is harvested in early to mid-October; late-harvested Rehmannia glutinosa is harvested from late October to early November; wild varieties can also be harvested in spring. When harvesting, dig carefully and deeply, being careful not to break the roots. Remove the stems, leaves, rhizomes, and fibrous roots, and wash away the soil to obtain fresh Rehmannia glutinosa. Alternatively, after digging it up, bury it in dry sand without washing it and store it in a dry, cool place. It can be stored for 2-3 months when needed.

[0035] [Medicinal Properties] Sweet, bitter, cold. Enters the Heart, Liver, and Kidney meridians.

[0036]

Functions and Indications

[0037] Rehmannia glutinosa

Alternative name

[0038]

Origin

[0039] [Original plant] Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch.

[0040] [Harvesting and Processing] Take dried Rehmannia glutinosa and add 30% by weight of rice wine, mix well, place in a steamer, steam until black and glossy inside and out, take out and sun-dry. Alternatively, take Rehmannia glutinosa, place it in a steamer and steam for 8 hours, then let it sit overnight. Turn it over the next day, steam for another 4-8 hours, let it sit overnight again, take it out, sun-dry until 80% dry, slice it, and then sun-dry it completely.

[0041] [Medicinal Properties] Sweet, warm. Enters the liver and kidney meridians.

[0042]

Functions and Indications

[0043] In a first aspect, the present invention provides a composition having the effect of replenishing qi and blood, which is made from the following raw materials in parts by weight: 5-15 parts of Angelica sinensis, 9-30 parts of Astragalus membranaceus and 7.5-15 parts of Rehmannia glutinosa; wherein the Angelica sinensis is a mixture of raw Angelica sinensis and vinegar-processed Angelica sinensis, the Astragalus membranaceus is a mixture of raw Astragalus membranaceus and honey-processed Astragalus membranaceus, and the Rehmannia glutinosa is a mixture of raw Rehmannia glutinosa and processed Rehmannia glutinosa.

[0044] The weight of Angelica sinensis is selected from any value or sub-range of 5-15 parts, including but not limited to the following values ​​or any range between the two: 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, and 15 parts.

[0045] The weight of Astragalus membranaceus is selected from any value or sub-range of 9-30 parts, including but not limited to the following values ​​or any range between two: 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19 parts, 19.5 parts, 20 parts, 20.5 parts, 21 parts, 21.5 parts, 22 parts, 22.5 parts, 23 parts, 23.5 parts, 24 parts, 24.5 parts, 25 parts, 25.5 parts, 26 parts, 26.5 parts, 27 parts, 27.5 parts, 28 parts, 28.5 parts, 29 parts, 29.5 parts, and 30 parts.

[0046] The weight of Rehmannia glutinosa is selected from any value or sub-range of 7.5-15 parts, including but not limited to the following values ​​or any range between the two: 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, 10.5 parts, 11 parts, 11.5 parts, 12 parts, 12.5 parts, 13 parts, 13.5 parts, 14 parts, 14.5 parts, and 15 parts.

[0047] Furthermore, the composition is made from the following raw materials in parts by weight: 12 parts Angelica sinensis, 30 parts Astragalus membranaceus, and 15 parts Rehmannia glutinosa.

[0048] Furthermore, the weight ratio of raw Angelica sinensis to vinegar-processed Angelica sinensis is 3:1-1:3; the weight ratio of raw Astragalus membranaceus to honey-processed Astragalus membranaceus is 2:1-1:3; and the weight ratio of raw Rehmannia glutinosa to processed Rehmannia glutinosa is 2:1-1:3.

[0049] Furthermore, the weight ratio of raw Angelica sinensis to vinegar-processed Angelica sinensis is 1:3; the weight ratio of raw Astragalus membranaceus to honey-processed Astragalus membranaceus is 1:1; and the weight ratio of raw Rehmannia glutinosa to processed Rehmannia glutinosa is 1:2.

[0050] Secondly, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: (1) Angelica sinensis was crushed and extracted by ultrasonication to obtain extract and Angelica sinensis residue. The extract was concentrated, precipitated with alcohol, and separated to obtain crude polysaccharide of Angelica sinensis and supernatant of alcohol precipitation of Angelica sinensis. (2) Pulverize Rehmannia glutinosa, extract it by ultrasound, and obtain the extract and Rehmannia glutinosa residue. Concentrate the extract, precipitate it with alcohol, and separate it to obtain crude Rehmannia glutinosa polysaccharide and Rehmannia glutinosa alcohol precipitation supernatant. (3) Pulverize Astragalus membranaceus, reflux and extract with water to obtain Astragalus membranaceus aqueous extract and Astragalus membranaceus residue. Reflux Astragalus membranaceus residue and extract with alcohol to obtain Astragalus membranaceus alcohol extract. Combine Astragalus membranaceus aqueous extract and Astragalus membranaceus alcohol extract, concentrate to obtain Astragalus membranaceus concentrate. (4) Mix the Angelica dahurica residue obtained in step (1) and the Rehmannia glutinosa residue obtained in step (2) with an alcohol-water solution, reflux to extract, take the supernatant, concentrate, and obtain Rehmannia glutinosa-Angelica dahurica compound concentrate. (5) Mix the crude polysaccharide of Angelica sinensis obtained in step (1) with the concentrated extract of Astragalus membranaceus obtained in step (3), shear emulsify and homogenize under pressure to obtain Astragalus membranaceus embedding solution; (6) Mix the Rehmannia polysaccharide obtained in step (2), the Astragalus embedding solution obtained in step (5), and the Rehmannia and Angelica compound concentrate obtained in step (4), shear emulsify, pressurize and homogenize, and then process to obtain the composition.

[0051] Further, in step (1), the angelica is pulverized and then sieved; the ultrasonic extraction includes the following steps: adding water to the angelica at a material-to-liquid ratio of 1:20-1:30 g / mL, and extracting for 30-45 min under ultrasonic power of 200-300W and temperature of 50-60℃; the concentration is to concentrate until the weight of the concentrate is 0.9-1.1 times the weight of the angelica; the alcohol precipitation includes the following steps: after cooling, adding anhydrous ethanol to 60%vol-80%vol, mixing well, and letting stand for 8-18 h; In step (2), the Rehmannia glutinosa is pulverized and sieved; the ultrasonic extraction includes the following steps: adding water to the Rehmannia glutinosa at a material-to-liquid ratio of 1:20-1:30 g / mL, and extracting for 30-45 min under ultrasonic power of 200-300W and temperature of 50-60℃; the concentration is to concentrate until the weight of the concentrate is 0.9-1.1 times the weight of the Rehmannia glutinosa; the alcohol precipitation includes the following steps: after cooling, adding anhydrous ethanol to 60%vol-80%vol, mixing well, and letting stand for 8-18 h; In step (3), the Astragalus membranaceus is pulverized and then sieved; the material-to-liquid ratio of the reflux water extraction is 1:20-1:30 g / mL, the temperature is 70-80℃, the number of extractions is 1-2 times, and the time is 1-1.5h; the solvent used in the reflux alcohol extraction is a 60%vol-80%vol ethanol aqueous solution, the material-to-liquid ratio of the reflux alcohol extraction is 1:10-1:20 g / mL, the temperature is 70-80℃, the number of extractions is 1-2 times, and the time is 1-1.5h; the concentration is to concentrate until the weight of the concentrated liquid is 0.9-1.1 times the weight of the Astragalus membranaceus. In step (4), the alcohol-water solution is a 50% vol-70% vol alcohol-water solution, the material-to-liquid ratio of the reflux extraction is 1:20-1:30 g / mL, the reflux extraction temperature is 50-60℃, the extraction is performed 1-2 times, and each extraction lasts 1-1.5 h; the concentration is performed until the weight of the concentrate is 0.9-1.1 times the sum of the weights of the Angelica sinensis residue and Rehmannia glutinosa residue. In step (5), the frequency of shear emulsification is 3000-5000 rpm and the time of shear emulsification is 5-10 min; the pressure of pressurization homogenization is 35-45 MPa and the number of pressurization homogenization cycles is 1-2. In step (6), the frequency of shear emulsification is 3000-5000 rpm and the time of shear emulsification is 5-10 min; the pressure of pressurization homogenization is 40-60 MPa and the number of pressurization homogenizations is 1-2 times.

[0052] Thirdly, the present invention provides a qi-tonifying and blood-nourishing medicine, comprising the above-described composition or the composition prepared by the above-described preparation method.

[0053] Fourthly, the present invention provides the use of the above-described composition or the composition prepared by the above-described preparation method in the preparation of qi-tonifying and blood-nourishing drugs.

[0054] Fifthly, the present invention provides the use of the above-described composition or the composition prepared by the above-described method in the preparation of medicaments for treating, adjuvant treating or preventing anemia.

[0055] In a sixth aspect, the present invention provides the use of the above-described composition or the composition prepared by the above-described method in the preparation of a medicament for treating, adjuvant treating or preventing hematopoietic dysfunction.

[0056] Based on further solutions to the technical problems of the present invention, or simultaneous solutions to multiple technical problems, the preferred solution in the technical solution provided in the first aspect of the present invention includes: The first preferred formulation is prepared from the following raw materials by weight: 12 parts Angelica sinensis, 30 parts Astragalus membranaceus, and 15 parts Rehmannia glutinosa. This formulation, based on the previous formulation's work of "increasing the levels of RBC, HGB, HCT, WBC, and PLT in anemic mice, and increasing the levels of EPO, G-CSF, M-CSF, and sVCAM-1 in the serum of anemic mice," further addresses the technical problem of "increasing the RBC level in anemic mice to 4.95 × 10⁻⁶". 12 / L, increased HGB level in anemic mice to 118.11 g / L, increased HCT level in anemic mice to 36.61%, and increased WBC level in anemic mice to 4.98 × 10⁹ / L. 9 / L, increased PLT levels in anemic mice to 495.54×10 9 The level of EPO in the serum of anemic mice was increased to 22.49 IU / L, the level of G-CSF in the serum of anemic mice was increased to 29.55 ng / L, the level of M-CSF in the serum of anemic mice was increased to 113.69 ng / L, and the level of sVCAM-1 in the serum of anemic mice was increased to 235.77 µg / L.

[0057] The second preferred option: the weight ratio of raw Angelica sinensis to vinegar-processed Angelica sinensis is 3:1-1:3; the weight ratio of raw Astragalus membranaceus to honey-processed Astragalus membranaceus is 2:1-1:3; and the weight ratio of raw Rehmannia glutinosa to processed Rehmannia glutinosa is 2:1-1:3. This technical solution, based on "increasing the levels of RBC, HGB, HCT, WBC, and PLT in anemic mice, and increasing the levels of EPO, G-CSF, M-CSF, and sVCAM-1 in the serum of anemic mice," further solves the technical problem of "increasing the RBC level in anemic mice to 4.72-4.95 × 10⁻⁶". 12 / L, increased HGB level in anemic mice to 106.41-118.11 g / L, increased HCT level in anemic mice to 31.95%-36.61%, and increased WBC level in anemic mice to 4.82-4.98×10 9 / L, increased PLT levels in anemic mice to 453.78-495.54×10 9 The study increased the levels of EPO in the serum of anemic mice to 21.99-22.49 IU / L, G-CSF to 25.94-29.55 ng / L, M-CSF to 108.90-113.69 ng / L, and sVCAM-1 to 219.84-235.77 µg / L.

[0058] The third preferred option: the weight ratio of raw Angelica sinensis to vinegar-processed Angelica sinensis is 1:3; the weight ratio of raw Astragalus membranaceus to honey-processed Astragalus membranaceus is 1:1; and the weight ratio of raw Rehmannia glutinosa to processed Rehmannia glutinosa is 1:2. This technical solution, based on "increasing the levels of RBC, HGB, HCT, WBC, and PLT in anemic mice, and increasing the levels of EPO, G-CSF, M-CSF, and sVCAM-1 in the serum of anemic mice," further solves the technical problem of "increasing the RBC level in anemic mice to 4.95 × 10⁻⁶". 12 / L, increased HGB level in anemic mice to 118.11 g / L, increased HCT level in anemic mice to 36.61%, and increased WBC level in anemic mice to 4.98 × 10⁹ / L. 9 / L, increased PLT levels in anemic mice to 495.54×10 9 The level of EPO in the serum of anemic mice was increased to 22.49 IU / L, the level of G-CSF in the serum of anemic mice was increased to 29.55 ng / L, the level of M-CSF in the serum of anemic mice was increased to 113.69 ng / L, and the level of sVCAM-1 in the serum of anemic mice was increased to 235.77 µg / L.

[0059] The beneficial effects of this invention are as follows: The present invention has at least the following beneficial effects: Compared with existing technologies, this invention has better technical effects in increasing the levels of RBC, HGB, HCT, WBC, and PLT in anemic mice, and in increasing the levels of EPO, G-CSF, M-CSF, and sVCAM-1 in the serum of anemic mice. At least, according to experimental tests, this invention increases the RBC count in anemic mice from 3.73 (using traditional Chinese medicine decoctions) to over 4.72 (units per 10⁻⁶). 12 According to experimental tests, this invention increases the hematocrit (HGB) in blood-deficient mice from 87.79 in traditional Chinese medicine decoctions to over 106.41 (g / L); according to experimental tests, this invention increases the hematocrit (HCT) in blood-deficient mice from 26.14% in traditional Chinese medicine decoctions to over 31.95%; according to experimental tests, this invention increases the white blood cell count (WBC) in blood-deficient mice from 3.66 in traditional Chinese medicine decoctions to over 4.82 (g / L). 9 / L); According to experimental tests, this invention increases the PLT in blood-deficient mice from 408.15 in traditional Chinese medicine decoctions to over 453.78 (units per 10^6). 9According to experimental tests, this invention increases the EPO level in anemic mice from 17.84 in traditional Chinese medicine decoctions to over 21.99 (unit IU / L); according to experimental tests, this invention increases the G-CSF level in anemic mice from 17.68 in traditional Chinese medicine decoctions to over 25.94 (unit ng / L); according to experimental tests, this invention increases the M-CSF level in anemic mice from 88.88 in traditional Chinese medicine decoctions to over 108.90 (unit ng / L); according to experimental tests, this invention increases the sVCAM-1 level in anemic mice from 168.62 in traditional Chinese medicine decoctions to over 219.84 (unit µg / L). Detailed Implementation

[0060] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0061] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all instruments, devices, equipment, reagents, products, etc., used in the embodiments of the present invention are obtained through conventional commercial means.

[0062] Example 1 (1) Preparation of crude polysaccharide of Angelica sinensis / crude polysaccharide of Rehmannia glutinosa: 3 parts of raw Angelica sinensis, 9 parts of vinegar-processed Angelica sinensis, 5 parts of raw Rehmannia glutinosa, and 10 parts of processed Rehmannia glutinosa were pulverized and passed through a 30-60 mesh sieve, washed, and pure water with a material-to-liquid ratio of 1:30 was added. The mixture was extracted for 45 min under ultrasonic power of 200W and temperature of 50℃. The supernatant was filtered and concentrated to an equal weight of the raw materials by rotary evaporation. After cooling, anhydrous ethanol was added to 70%. After thorough mixing, the mixture was allowed to stand for 12 hours. The precipitate was collected by centrifugation to obtain crude polysaccharide of Angelica sinensis / crude polysaccharide of Rehmannia glutinosa. The alcohol and the residue were collected for later use.

[0063] (2) Preparation of Astragalus extract (polysaccharides, crude saponins, and isoflavones): 15 parts of raw Astragalus and 15 parts of honey-processed Astragalus were pulverized and passed through a 30-60 mesh sieve. Pure water with a material-to-liquid ratio of 1:30 was added, and the mixture was refluxed once at 70℃ for 1 hour. The aqueous extract was then filtered. 70% ethanol with a material-to-liquid ratio of 1:10 was added to the residue, and the mixture was refluxed twice at 80℃ for 1.5 hours each time. The alcoholic extract was then filtered. The aqueous and alcoholic extracts were combined and concentrated by rotary evaporation to a concentration equal in weight to the raw materials to obtain Astragalus concentrate. (3) Preparation of alcoholic extracts of Rehmannia glutinosa and Angelica sinensis (ferulic acid and calool): Combine the extract residues of Angelica sinensis and Astragalus membranaceus, add a 60% alcohol solution with a material-to-liquid ratio of 1:30 (first add the alcohol solution collected in step (1), and make up the rest with 95% alcohol and pure water to make the final alcohol concentration of the system 60%), and reflux extract twice at 50℃, 1 h each time. Filter to obtain the supernatant, and concentrate by rotary evaporation to obtain a concentrated solution equal in weight to the raw materials to obtain the Rehmannia glutinosa and Angelica sinensis compound concentrated solution.

[0064] (4) Preparation of Astragalus embedding solution: Mix Angelica sinensis crude polysaccharide and Astragalus membranaceus concentrate thoroughly, emulsify at 5000 rpm for 5 min, homogenize under high pressure at 45 MPa once, cool down, solidify, and form a gel matrix to obtain Astragalus embedding solution.

[0065] (5) Preparation of Astragalus-Rehmannia-Angelica encapsulation: The crude polysaccharide of Rehmannia, the encapsulation solution of Astragalus, and the concentrated solution of Rehmannia and Angelica were thoroughly mixed, emulsified at 5000 rpm for 5 min, and homogenized under high pressure at 60 MPa once to concentrate into a paste or spray dried to obtain the product.

[0066] Example 2 (1) Preparation of crude polysaccharide from Angelica sinensis / crude polysaccharide from Rehmannia glutinosa: 3.75 parts of raw Angelica sinensis, 1.25 parts of vinegar-processed Angelica sinensis, 5 parts of raw Rehmannia glutinosa, and 2.5 parts of processed Rehmannia glutinosa were pulverized and passed through a 30-60 mesh sieve, washed, and pure water with a material-to-liquid ratio of 1:20 was added. The mixture was extracted for 30 min under ultrasonic power of 300W and temperature of 60℃. The supernatant was filtered and concentrated to an equal weight of the raw materials by rotary evaporation. After cooling, anhydrous ethanol was added to 70%. After thorough mixing, the mixture was allowed to stand for 12 h. The precipitate was collected by centrifugation to obtain crude polysaccharide from Angelica sinensis / crude polysaccharide from Rehmannia glutinosa. The alcohol and residue were collected for later use.

[0067] (2) Preparation of Astragalus extract (polysaccharides, crude saponins, and isoflavones): 6 parts raw Astragalus and 3 parts honey-processed Astragalus were pulverized and passed through a 30-60 mesh sieve. Pure water with a material-to-liquid ratio of 1:20 was added, and the mixture was refluxed once at 80℃ for 1.5 hours. The aqueous extract was then filtered. 70% ethanol with a material-to-liquid ratio of 1:20 was added to the residue, and the mixture was refluxed twice at 70℃ for 1 hour each time. The alcohol extract was then filtered. The aqueous and alcohol extracts were combined and concentrated by rotary evaporation to a concentration equal in weight to the raw materials to obtain Astragalus concentrate.

[0068] (3) Preparation of alcohol extracts of Rehmannia glutinosa and Angelica sinensis (ferulic acid and catalpol): Combine the extract residues of Angelica sinensis and Astragalus membranaceus, add a 60% alcohol solution with a material-to-liquid ratio of 1:20 (first add the alcohol solution collected in step (1), and make up the rest with 95% alcohol and pure water to make the final alcohol concentration of the system 60%), and reflux extract twice at 60℃, each time for 1.5 h. Filter to obtain the supernatant, and concentrate by rotary evaporation to obtain a concentrated solution equal in weight to the raw materials to obtain the Rehmannia glutinosa and Angelica sinensis compound concentrated solution.

[0069] (4) Preparation of Astragalus embedding solution: The crude polysaccharide of Angelica sinensis and the concentrated Astragalus membranaceus were thoroughly mixed, emulsified at 3000 rpm for 10 min, homogenized under high pressure at 40 MPa twice, cooled and solidified to form a gel matrix, and the Astragalus embedding solution was obtained.

[0070] (5) Preparation of Astragalus-Rehmannia-Angelica encapsulation: The crude polysaccharide of Rehmannia, the encapsulation solution of Astragalus, and the concentrated solution of Rehmannia and Angelica were thoroughly mixed, emulsified at 3000 rpm for 10 min, and homogenized twice under high pressure at 40 MPa to concentrate into a paste or spray dried to obtain the product.

[0071] Example 3 (1) Preparation of crude polysaccharide from Angelica sinensis / crude polysaccharide from Rehmannia glutinosa: 3 parts of raw Angelica sinensis, 6 parts of vinegar-processed Angelica sinensis, 2.25 parts of raw Rehmannia glutinosa, and 6.75 parts of processed Rehmannia glutinosa were pulverized and passed through a 30-60 mesh sieve, washed, and pure water with a material-to-liquid ratio of 1:25 was added. The mixture was extracted for 40 min under ultrasonic power of 250W and temperature of 55℃. The supernatant was filtered and concentrated to an equal weight of the raw materials by rotary evaporation. After cooling, anhydrous ethanol was added to 70%. The mixture was thoroughly mixed and allowed to stand for 12 h. The precipitate was collected by centrifugation to obtain crude polysaccharide from Angelica sinensis / crude polysaccharide from Rehmannia glutinosa. The alcohol and residue were collected for later use.

[0072] (2) Preparation of Astragalus extract (polysaccharides, crude saponins, and isoflavones): 5 parts raw Astragalus and 15 parts honey-processed Astragalus were pulverized through a 30-60 mesh sieve, and pure water at a ratio of 1:25 was added. The mixture was refluxed once at 75℃ for 1.3 hours, and the aqueous extract was obtained by filtration. 70% ethanol at a ratio of 1:15 was added to the residue, and the mixture was refluxed twice at 75℃ for 1.3 hours each time. The ethanol extract was obtained by filtration. The aqueous and ethanol extracts were combined and concentrated by rotary evaporation to a concentration equal in weight to the raw materials to obtain Astragalus concentrate.

[0073] (3) Preparation of alcohol extracts of Rehmannia glutinosa and Angelica sinensis (ferulic acid and catalpol): Combine the extract residues of Angelica sinensis and Astragalus membranaceus, add a 60% alcohol solution with a material-to-liquid ratio of 1:25 (first add the alcohol solution collected in step (1), and make up the rest with 95% alcohol and pure water to make the final alcohol concentration of the system 60%), and reflux extract twice at 55℃, each time for 1.3 h. Filter to obtain the supernatant, and concentrate by rotary evaporation to obtain a concentrated solution equal in weight to the raw materials to obtain the Rehmannia glutinosa and Angelica sinensis compound concentrated solution.

[0074] (4) Preparation of Astragalus embedding solution: Mix Angelica sinensis crude polysaccharide and Astragalus concentrate thoroughly, emulsify at 4000 rpm for 8 min, homogenize under high pressure at 35 MPa twice, cool down, solidify, and form a gel matrix to obtain Astragalus embedding solution.

[0075] (5) Preparation of Astragalus-Rehmannia-Angelica encapsulation: The crude polysaccharide of Rehmannia, the encapsulation solution of Astragalus, and the concentrated solution of Rehmannia and Angelica were thoroughly mixed, emulsified at 4000 rpm for 8 min, and homogenized twice under high pressure at 50 MPa to concentrate into a paste or spray dried to obtain the product.

[0076] Example 4 (1) Preparation of crude polysaccharide of Angelica sinensis / crude polysaccharide of Rehmannia glutinosa: 10 parts of raw Angelica sinensis, 5 parts of vinegar-processed Angelica sinensis, 6 parts of raw Rehmannia glutinosa, and 6 parts of processed Rehmannia glutinosa were pulverized and passed through a 30-60 mesh sieve, washed, and pure water with a material-to-liquid ratio of 1:28 was added. The mixture was extracted for 35 min under ultrasonic power of 270W and temperature of 60℃. The supernatant was filtered and concentrated to an equal weight of the raw materials by rotary evaporation. After cooling, anhydrous ethanol was added to 70%. After thorough mixing, the mixture was allowed to stand for 12 hours. The precipitate was collected by centrifugation to obtain crude polysaccharide of Angelica sinensis / crude polysaccharide of Rehmannia glutinosa. The alcohol and the residue were collected for later use.

[0077] (2) Preparation of Astragalus extract (polysaccharides, crude saponins, and isoflavones): 15 parts of raw Astragalus and 10 parts of honey-processed Astragalus were pulverized and passed through a 30-60 mesh sieve. Pure water with a material-to-liquid ratio of 1:28 was added, and the mixture was refluxed once at 72℃ for 1.5 h. The aqueous extract was then filtered. 70% ethanol with a material-to-liquid ratio of 1:18 was added to the residue, and the mixture was refluxed twice at 80℃ for 1.5 h each time. The ethanol extract was then filtered. The aqueous and ethanol extracts were combined and concentrated by rotary evaporation to a concentration equal in weight to the raw materials to obtain Astragalus concentrate.

[0078] (4) Preparation of alcoholic extracts of Rehmannia glutinosa and Angelica sinensis (ferulic acid and catalpol): Combine the extract residues of Angelica sinensis and Astragalus membranaceus, add a 60% alcohol solution with a material-to-liquid ratio of 1:28 (first add the alcohol solution collected in step (1), and make up the rest with 95% alcohol and pure water to make the final alcohol concentration of the system 60%), and reflux extract twice at 60℃, each time for 1.5 h. Filter to obtain the supernatant, and concentrate by rotary evaporation to obtain a concentrated solution equal in weight to the raw materials to obtain the Rehmannia glutinosa and Angelica sinensis compound concentrated solution.

[0079] (4) Preparation of Astragalus embedding solution: The crude polysaccharide of Angelica sinensis and the concentrated Astragalus membranaceus were thoroughly mixed, emulsified at 4500 rpm for 9 min, homogenized under high pressure at 45 MPa for 2 times, cooled and solidified to form a gel matrix, and the Astragalus embedding solution was obtained.

[0080] (5) Preparation of Astragalus-Rehmannia-Angelica encapsulation: The crude polysaccharide of Rehmannia, the encapsulation solution of Astragalus, and the concentrated solution of Rehmannia and Angelica were thoroughly mixed, emulsified at 4500 rpm for 9 min, and homogenized twice under high pressure at 45 MPa to concentrate into a paste or spray dried to obtain the product.

[0081] Example 5 (1) Preparation of crude polysaccharide of Angelica sinensis / crude polysaccharide of Rehmannia glutinosa: 4 parts of raw Angelica sinensis, 4 parts of vinegar-processed Angelica sinensis, 7 parts of raw Rehmannia glutinosa, and 7 parts of processed Rehmannia glutinosa were pulverized and passed through a 30-60 mesh sieve, washed, and pure water with a material-to-liquid ratio of 1:20 was added. The mixture was extracted for 30 min under ultrasonic power of 300W and temperature of 60℃. The supernatant was filtered and concentrated to an equal weight of the raw materials by rotary evaporation. After cooling, anhydrous ethanol was added to 70%. After thorough mixing, the mixture was allowed to stand for 12 h. The precipitate was collected by centrifugation to obtain crude polysaccharide of Angelica sinensis / crude polysaccharide of Rehmannia glutinosa. The alcohol and the residue were collected for later use.

[0082] (2) Preparation of Astragalus extract (polysaccharides, crude saponins, and isoflavones): 8 parts raw Astragalus and 8 parts honey-processed Astragalus were pulverized and passed through a 30-60 mesh sieve. Pure water with a material-to-liquid ratio of 1:20 was added, and the mixture was refluxed once at 80℃ for 1.5 hours. The aqueous extract was then filtered. 70% ethanol with a material-to-liquid ratio of 1:20 was added to the residue, and the mixture was refluxed twice at 70℃ for 1 hour each time. The alcohol extract was then filtered. The aqueous and alcohol extracts were combined and concentrated by rotary evaporation to a concentration equal in weight to the raw materials to obtain Astragalus concentrate.

[0083] (3) Preparation of alcohol extracts of Rehmannia glutinosa and Angelica sinensis (ferulic acid and catalpol): Combine the extract residues of Angelica sinensis and Astragalus membranaceus, add a 60% alcohol solution with a material-to-liquid ratio of 1:20 (first add the alcohol solution collected in step (1), and make up the rest with 95% alcohol and pure water to make the final alcohol concentration of the system 60%), and reflux extract twice at 60℃, each time for 1.5 h. Filter to obtain the supernatant, and concentrate by rotary evaporation to obtain a concentrated solution equal in weight to the raw materials to obtain the Rehmannia glutinosa and Angelica sinensis compound concentrated solution.

[0084] (4) Preparation of Astragalus embedding solution: The crude polysaccharide of Angelica sinensis and the concentrated Astragalus membranaceus were thoroughly mixed, emulsified at 3000 rpm for 10 min, homogenized under high pressure at 40 MPa twice, cooled and solidified to form a gel matrix, and the Astragalus embedding solution was obtained.

[0085] (5) Preparation of Astragalus-Rehmannia-Angelica encapsulation: The crude polysaccharide of Rehmannia, the encapsulation solution of Astragalus, and the concentrated solution of Rehmannia and Angelica were thoroughly mixed, emulsified at 3000 rpm for 10 min, and homogenized twice under high pressure at 40 MPa to concentrate into a paste or spray dried to obtain the product.

[0086] The weight parts and weight ratios of Astragalus membranaceus, Rehmannia glutinosa, and Angelica sinensis used in Examples 1-5 are summarized in Table 1 below: Table 1

[0087] Comparative Example 1 Compared to Example 1, the formula composition is the same, processed according to existing traditional Chinese medicine decoction methods. Specifically, 3 parts raw Angelica sinensis, 9 parts vinegar-processed Angelica sinensis, 5 parts raw Rehmannia glutinosa, 10 parts prepared Rehmannia glutinosa, 15 parts raw Astragalus membranaceus, and 15 parts honey-processed Astragalus membranaceus are pulverized through a 30-60 mesh screen. Water is added to a level 2-3 cm above the surface of the herbs, and the herbs are soaked for 60 minutes. First decoction: After boiling, reduce to a simmer and cook for 30 minutes. Filter the liquid through a sieve, leaving the dregs in the pot. Second decoction: Add fresh water to the dregs until it reaches a level 1-2 cm above the surface of the herbs. Bring to a boil over high heat, then reduce to a simmer and cook for 20 minutes. Filter the liquid through a sieve. Combine the two filtrates, concentrate, and spray-dry to obtain a powder.

[0088] Comparative Example 2 Compared with Example 1, the only difference is that after completing steps 1-3, the crude polysaccharide of Angelica sinensis, crude polysaccharide of Rehmannia glutinosa, concentrated extract of Astragalus membranaceus, and concentrated extract of Rehmannia glutinosa and Angelica sinensis are combined, mixed evenly, and then directly spray-dried to obtain powder.

[0089] Comparative Example 3 Compared with Example 1, the only difference is that after completing steps 1-3, the crude polysaccharide of Angelica sinensis, crude polysaccharide of Rehmannia glutinosa, concentrated extract of Astragalus membranaceus, and concentrated extract of Rehmannia glutinosa and Angelica sinensis are combined and mixed, emulsified at 5000 rpm for 5 min, homogenized under high pressure at 60 MPa once, concentrated, and spray-dried to obtain powder.

[0090] Comparative Example 4 Compared with Example 1, the only difference is that all the Angelica sinensis, Rehmannia glutinosa, and Astragalus membranaceus are raw, i.e., 12 parts of raw Angelica sinensis, 15 parts of raw Rehmannia glutinosa, and 30 parts of raw Astragalus membranaceus. The rest of the process and parameters are the same as in Example 1.

[0091] Comparative Example 5 Compared with Example 1, the only difference is that all Angelica sinensis is processed with vinegar, all Rehmannia glutinosa is processed with prepared Rehmannia glutinosa, and all Astragalus membranaceus is processed with honey, that is, 12 parts of vinegar-processed Angelica sinensis, 15 parts of prepared Rehmannia glutinosa, and 30 parts of honey-processed Astragalus membranaceus. The remaining processes and parameters are the same as in Example 1.

[0092] Effect evaluation 1. Evaluation of hematopoietic function in mice 1.1 Experimental Methods One hundred and forty-four healthy 6-8 week old male ICR mice, weighing (20±2) g, were randomly divided into four groups of 12 mice each: control group, model group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group. After 5 days of acclimatization, except for the control group, the other mice were intraperitoneally injected with cyclophosphamide at a dose of 80 mg / kg for 2 consecutive days to establish an anemia model in experimental mice. Astragalus-Rehmannia-Angelica encapsulated powder prepared according to Examples 1-5 was administered by gavage at a dose of 0.4 g / kg mouse body weight, and Comparative Examples 1-5 were administered by gavage at a dose of 0.4 g / kg mouse body weight (calculated by powder measurement) once a day for 7 consecutive days.

[0093] Thirty minutes after the last gavage administration to mice, blood was collected from the circumferential vein. Immediately, 200 µL of blood was drawn and placed in an EP tube containing EDTA anticoagulant. The tube was gently shaken to mix thoroughly with the anticoagulant. Blood parameters including red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), white blood cells (WBC), and platelets (PLT) were measured using an automated blood analyzer. Three mL of blood was collected from the mouse abdominal aorta and placed in an EDTA vacuum blood collection tube. After standing at room temperature for 60 minutes, the blood was centrifuged at 3000 rpm at low temperature (4℃) for 15 minutes. The supernatant was collected, placed in a centrifuge tube, sealed, and stored at -20℃ for later analysis. An appropriate amount of mouse plasma was collected for the determination of erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF), and soluble vascular cell adhesion molecule-1 (sVCAM-1) indicators, following the ELISA kit instructions.

[0094] 1.2 Experimental Results Table 2 Results of peripheral blood parameters RBC, HGB, HCT, WBC and PLT in mice of each experimental group

[0095] Note: Compared with the model group, *P<0.05;**P<0.01;***P<0.001 Compared with the blank control group, the peripheral blood parameters RBC, HGB, HCT, WBC, and PLT in the model group, the example group, and each comparative group were significantly lower than those in the blank group, indicating that the cyclophosphamide-established blood deficiency mouse model was successful. Compared with the model group, the RBC, HGB, HCT, WBC, and PLT values ​​in the example group and each comparative group were all increased and showed significant differences from those in the model group, indicating that the combination of Angelica sinensis, Rehmannia glutinosa, and Astragalus membranaceus has a good qi- and blood-tonifying effect. Among them, the RBC, HGB, HCT, WBC, and PLT values ​​in the example groups 1-5 were all higher than those in the comparative groups, indicating that the combination of different raw material processing methods and double-layer embedding treatment can significantly improve the qi- and blood-tonifying effect of the composition. Among the groups of Examples 1-5, the group of Example 1 showed the best effect, indicating that the combination of 12 parts Angelica sinensis, 30 parts Astragalus membranaceus and 15 parts Rehmannia glutinosa, with the following weight ratios for replenishing qi and blood: raw Angelica sinensis and vinegar-processed Angelica sinensis in 1:3, raw Astragalus membranaceus and honey-processed Astragalus membranaceus in 1:1, and raw Rehmannia glutinosa and processed Rehmannia glutinosa in 1:2.

[0096] Table 3. Results of serum marker levels in mice of each experimental group

[0097] Note: Compared with the model group, *P<0.05;**P<0.01;***P<0.001 Erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF), and soluble vascular cell adhesion molecule-1 (sVCAM-1) are the main hematopoietic growth factors regulating hematopoietic function. Compared with the blank control group, the serum levels of erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF), and soluble vascular cell adhesion molecule-1 (sVCAM-1) in the model group, the example group, and each comparative proportion group were significantly reduced, suggesting that cyclophosphamide inhibits the synthesis and secretion of EPO and the synthesis of CSF, and can reduce the levels of M-CSF and sVCAM-1, thereby impairing hematopoietic function in mice. Compared to the model group, the EPO, G-CSF, M-CSF, and sVCAM-1 values ​​in the example groups and each comparative group were all increased and showed significant differences from the model group, indicating that the combination of Angelica sinensis, Rehmannia glutinosa, and Astragalus membranaceus has a significant promoting effect on the body's hematopoietic function. In particular, the EPO, G-CSF, M-CSF, and sVCAM-1 values ​​in Example 1-Example 5 were all higher than those in the comparative groups, indicating that combining different processing methods of raw materials with double-layer encapsulation can significantly improve the hematopoietic function of the composition.

[0098] Among the groups of Examples 1-5, the group of Example 1 showed the best effect, indicating that the combination of 12 parts Angelica sinensis, 30 parts Astragalus membranaceus and 15 parts Rehmannia glutinosa, with the following weight ratios for replenishing qi and blood: raw Angelica sinensis and vinegar-processed Angelica sinensis in 1:3, raw Astragalus membranaceus and honey-processed Astragalus membranaceus in 1:1, and raw Rehmannia glutinosa and processed Rehmannia glutinosa in 1:2.

[0099] In summary, the angelica, rehmannia and astragalus encapsulated composition prepared by the method of the present invention has a good enhancing effect on replenishing qi and blood.

[0100] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition having the effect of replenishing qi and blood, characterized in that, It is made from the following raw materials in parts by weight: 5-15 parts Angelica sinensis, 9-30 parts Astragalus membranaceus and 7.5-15 parts Rehmannia glutinosa; The Angelica sinensis is a mixture of raw Angelica sinensis and vinegar-processed Angelica sinensis, the Astragalus membranaceus is a mixture of raw Astragalus membranaceus and honey-processed Astragalus membranaceus, and the Rehmannia glutinosa is a mixture of raw Rehmannia glutinosa and processed Rehmannia glutinosa.

2. The composition according to claim 1, characterized in that, It is made from the following ingredients by weight: 12 parts Angelica sinensis, 30 parts Astragalus membranaceus, and 15 parts Rehmannia glutinosa.

3. The composition according to claim 1, characterized in that, The weight ratio of raw Angelica sinensis to vinegar-processed Angelica sinensis is 3:1-1:3; the weight ratio of raw Astragalus membranaceus to honey-processed Astragalus membranaceus is 2:1-1:3; and the weight ratio of raw Rehmannia glutinosa to processed Rehmannia glutinosa is 2:1-1:

3.

4. The composition according to claim 3, characterized in that, The weight ratio of raw Angelica sinensis to vinegar-processed Angelica sinensis is 1:3; the weight ratio of raw Astragalus membranaceus to honey-processed Astragalus membranaceus is 1:1; and the weight ratio of raw Rehmannia glutinosa to processed Rehmannia glutinosa is 1:

2.

5. A method for preparing the composition according to any one of claims 1-4, characterized in that, Includes the following steps: (1) Angelica sinensis was crushed and extracted by ultrasonication to obtain extract and Angelica sinensis residue. The extract was concentrated, precipitated with alcohol, and separated to obtain crude polysaccharide of Angelica sinensis and supernatant of alcohol precipitation of Angelica sinensis. (2) Pulverize Rehmannia glutinosa, extract it by ultrasound, and obtain the extract and Rehmannia glutinosa residue. Concentrate the extract, precipitate it with alcohol, and separate it to obtain crude Rehmannia glutinosa polysaccharide and Rehmannia glutinosa alcohol precipitation supernatant. (3) Pulverize Astragalus membranaceus, reflux and extract with water to obtain Astragalus membranaceus aqueous extract and Astragalus membranaceus residue. Reflux Astragalus membranaceus residue and extract with alcohol to obtain Astragalus membranaceus alcohol extract. Combine Astragalus membranaceus aqueous extract and Astragalus membranaceus alcohol extract, concentrate to obtain Astragalus membranaceus concentrate. (4) Mix the Angelica dahurica residue obtained in step (1) and the Rehmannia glutinosa residue obtained in step (2) with an alcoholic aqueous solution, reflux extract, take the supernatant, concentrate, and obtain Rehmannia glutinosa and Angelica dahurica compound concentrate. (5) Mix the crude polysaccharide of Angelica sinensis obtained in step (1) with the concentrated extract of Astragalus membranaceus obtained in step (3), shear emulsify and homogenize under pressure to obtain Astragalus membranaceus embedding solution; (6) Mix the Rehmannia polysaccharide obtained in step (2), the Astragalus embedding solution obtained in step (5), and the Rehmannia and Angelica compound concentrate obtained in step (4), shear emulsify, pressurize and homogenize, and then process to obtain the composition.

6. The preparation method according to claim 5, characterized in that, In step (1), the angelica is pulverized and sieved; the ultrasonic extraction includes the following steps: adding water to the angelica at a material-to-liquid ratio of 1:20-1:30 g / mL, and extracting for 30-45 min under ultrasonic power of 200-300W and temperature of 50-60℃; the concentration is to concentrate until the weight of the concentrate is 0.9-1.1 times the weight of the angelica; the alcohol precipitation includes the following steps: after cooling, adding anhydrous ethanol to 60%vol-80%vol, mixing well, and letting stand for 8-18 h; In step (2), the Rehmannia glutinosa is pulverized and sieved; the ultrasonic extraction includes the following steps: adding water to the Rehmannia glutinosa at a material-to-liquid ratio of 1:20-1:30 g / mL, and extracting for 30-45 min under ultrasonic power of 200-300W and temperature of 50-60℃; the concentration is to concentrate until the weight of the concentrate is 0.9-1.1 times the weight of the Rehmannia glutinosa; the alcohol precipitation includes the following steps: after cooling, adding anhydrous ethanol to 60%vol-80%vol, mixing well, and letting stand for 8-18 h; In step (3), the Astragalus membranaceus is pulverized and then sieved; the material-to-liquid ratio of the reflux water extraction is 1:20-1:30 g / mL, the temperature is 70-80℃, the number of extractions is 1-2 times, and the time is 1-1.5h; the solvent used in the reflux alcohol extraction is a 60%vol-80%vol ethanol aqueous solution, the material-to-liquid ratio of the reflux alcohol extraction is 1:10-1:20 g / mL, the temperature is 70-80℃, the number of extractions is 1-2 times, and the time is 1-1.5h; the concentration is to concentrate until the weight of the concentrated liquid is 0.9-1.1 times the weight of the Astragalus membranaceus. In step (4), the alcohol-water solution is a 50% vol-70% vol alcohol-water solution, the material-to-liquid ratio of the reflux extraction is 1:20-1:30 g / mL, the reflux extraction temperature is 50-60℃, the extraction is performed 1-2 times, and each extraction lasts 1-1.5 h; the concentration is performed until the weight of the concentrate is 0.9-1.1 times the sum of the weights of the Angelica sinensis residue and Rehmannia glutinosa residue. In step (5), the frequency of shear emulsification is 3000-5000 rpm and the time of shear emulsification is 5-10 min; the pressure of pressurization homogenization is 35-45 MPa and the number of pressurization homogenization cycles is 1-2. In step (6), the frequency of shear emulsification is 3000-5000 rpm and the time of shear emulsification is 5-10 min; the pressure of pressurization homogenization is 40-60 MPa and the number of pressurization homogenizations is 1-2 times.

7. A qi- and blood-tonifying medicine, characterized in that, This includes the composition according to any one of claims 1-4 or the composition prepared by the method according to any one of claims 5-6.

8. Use of the composition according to any one of claims 1-4 or the composition prepared by the preparation method according to any one of claims 5-6 in the preparation of qi-tonifying and blood-nourishing drugs.

9. Use of the composition according to any one of claims 1-4 or the composition prepared by the method according to any one of claims 5-6 in the preparation of a medicament for treating, adjunctive treating or preventing anemia.

10. Use of the composition according to any one of claims 1-4 or the composition prepared by any one of claims 5-6 in the preparation of a medicament for treating, adjuvant treating or preventing hematopoietic dysfunction.

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